CN105724293B - A kind of method with medical stone bucket High Density Cultivation Perna viridis larva - Google Patents
A kind of method with medical stone bucket High Density Cultivation Perna viridis larva Download PDFInfo
- Publication number
- CN105724293B CN105724293B CN201610127343.3A CN201610127343A CN105724293B CN 105724293 B CN105724293 B CN 105724293B CN 201610127343 A CN201610127343 A CN 201610127343A CN 105724293 B CN105724293 B CN 105724293B
- Authority
- CN
- China
- Prior art keywords
- algae
- parts
- larva
- bucket
- algaes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000004575 stone Substances 0.000 title claims abstract description 39
- 241001245601 Perna viridis Species 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 27
- 241000195493 Cryptophyta Species 0.000 claims abstract description 116
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 55
- 239000013535 sea water Substances 0.000 claims abstract description 19
- 239000012153 distilled water Substances 0.000 claims abstract description 14
- 238000012258 culturing Methods 0.000 claims abstract description 8
- 230000009571 larval growth Effects 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 239000007789 gas Substances 0.000 claims description 17
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 12
- 230000012010 growth Effects 0.000 claims description 10
- 238000005286 illumination Methods 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 10
- -1 polyethylene Polymers 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 229920000573 polyethylene Polymers 0.000 claims description 8
- 239000004698 Polyethylene Substances 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 238000005273 aeration Methods 0.000 claims description 6
- 239000001569 carbon dioxide Substances 0.000 claims description 6
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 6
- 210000002700 urine Anatomy 0.000 claims description 6
- 241000237852 Mollusca Species 0.000 claims description 5
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims description 5
- 230000000366 juvenile effect Effects 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 239000010936 titanium Substances 0.000 claims description 5
- 229910052719 titanium Inorganic materials 0.000 claims description 5
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 4
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 claims description 4
- 239000007836 KH2PO4 Substances 0.000 claims description 4
- 229910021586 Nickel(II) chloride Inorganic materials 0.000 claims description 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 4
- 238000004378 air conditioning Methods 0.000 claims description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 4
- 238000009395 breeding Methods 0.000 claims description 4
- 230000001488 breeding effect Effects 0.000 claims description 4
- 239000001110 calcium chloride Substances 0.000 claims description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 4
- 239000004202 carbamide Substances 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 4
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 4
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 4
- QMMRZOWCJAIUJA-UHFFFAOYSA-L nickel dichloride Chemical compound Cl[Ni]Cl QMMRZOWCJAIUJA-UHFFFAOYSA-L 0.000 claims description 4
- 150000003839 salts Chemical class 0.000 claims description 4
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 229910052911 sodium silicate Inorganic materials 0.000 claims description 4
- 239000013589 supplement Substances 0.000 claims description 4
- 241000209140 Triticum Species 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims description 3
- 229910021529 ammonia Inorganic materials 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 238000003501 co-culture Methods 0.000 claims description 3
- 230000003750 conditioning effect Effects 0.000 claims description 3
- 239000000839 emulsion Substances 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 230000029052 metamorphosis Effects 0.000 claims description 3
- 238000009343 monoculture Methods 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 3
- 229960000344 thiamine hydrochloride Drugs 0.000 claims description 3
- 235000019190 thiamine hydrochloride Nutrition 0.000 claims description 3
- 239000011747 thiamine hydrochloride Substances 0.000 claims description 3
- 241001072261 Musculista senhousia Species 0.000 claims description 2
- NNBFNNNWANBMTI-UHFFFAOYSA-M brilliant green Chemical compound OS([O-])(=O)=O.C1=CC(N(CC)CC)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](CC)CC)C=C1 NNBFNNNWANBMTI-UHFFFAOYSA-M 0.000 claims description 2
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 2
- 235000012054 meals Nutrition 0.000 claims description 2
- 238000012549 training Methods 0.000 claims description 2
- 239000004744 fabric Substances 0.000 claims 1
- 210000003746 feather Anatomy 0.000 claims 1
- 238000011160 research Methods 0.000 abstract description 5
- 238000009472 formulation Methods 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 abstract description 3
- 238000009360 aquaculture Methods 0.000 abstract description 2
- 244000144974 aquaculture Species 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 13
- 241000237536 Mytilus edulis Species 0.000 description 11
- 235000020638 mussel Nutrition 0.000 description 11
- 235000015170 shellfish Nutrition 0.000 description 7
- 239000000645 desinfectant Substances 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 239000002131 composite material Substances 0.000 description 4
- 241000411851 herbal medicine Species 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000011435 rock Substances 0.000 description 4
- 239000000460 chlorine Substances 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 3
- 230000001418 larval effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 235000010755 mineral Nutrition 0.000 description 3
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 3
- 241001645380 Bassia scoparia Species 0.000 description 2
- 241000579895 Chlorostilbon Species 0.000 description 2
- 240000000774 Cunila origanoides Species 0.000 description 2
- 235000018274 Cunila origanoides Nutrition 0.000 description 2
- 235000014866 Dictamnus albus Nutrition 0.000 description 2
- 235000014043 Glechoma hederacea var parviflora Nutrition 0.000 description 2
- 206010018498 Goitre Diseases 0.000 description 2
- 240000002045 Guettarda speciosa Species 0.000 description 2
- 235000001287 Guettarda speciosa Nutrition 0.000 description 2
- 240000000950 Hippophae rhamnoides Species 0.000 description 2
- 235000003145 Hippophae rhamnoides Nutrition 0.000 description 2
- 235000013717 Houttuynia Nutrition 0.000 description 2
- 240000000691 Houttuynia cordata Species 0.000 description 2
- 244000246386 Mentha pulegium Species 0.000 description 2
- 235000016257 Mentha pulegium Nutrition 0.000 description 2
- 235000004357 Mentha x piperita Nutrition 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 244000215554 Nepeta hederacea Species 0.000 description 2
- 241000123107 Phellinus Species 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 230000005791 algae growth Effects 0.000 description 2
- 239000010231 banlangen Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 208000001848 dysentery Diseases 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229910052876 emerald Inorganic materials 0.000 description 2
- 239000010976 emerald Substances 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 201000003872 goiter Diseases 0.000 description 2
- 235000001050 hortel pimenta Nutrition 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 230000005415 magnetization Effects 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 230000004899 motility Effects 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241001501873 Isochrysis galbana Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- KKCBUQHMOMHUOY-UHFFFAOYSA-N Na2O Inorganic materials [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 description 1
- 241000159660 Nannochloropsis oculata Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- CSDREXVUYHZDNP-UHFFFAOYSA-N alumanylidynesilicon Chemical compound [Al].[Si] CSDREXVUYHZDNP-UHFFFAOYSA-N 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 239000005667 attractant Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000031902 chemoattractant activity Effects 0.000 description 1
- 239000003653 coastal water Substances 0.000 description 1
- 229910052681 coesite Inorganic materials 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229910052593 corundum Inorganic materials 0.000 description 1
- 229910052906 cristobalite Inorganic materials 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N iron oxide Inorganic materials [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 239000012633 leachable Substances 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 description 1
- 229940107698 malachite green Drugs 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- BBJSDUUHGVDNKL-UHFFFAOYSA-J oxalate;titanium(4+) Chemical compound [Ti+4].[O-]C(=O)C([O-])=O.[O-]C(=O)C([O-])=O BBJSDUUHGVDNKL-UHFFFAOYSA-J 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 229910052761 rare earth metal Inorganic materials 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 229910052682 stishovite Inorganic materials 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000000515 tooth Anatomy 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 229910052905 tridymite Inorganic materials 0.000 description 1
- 229910001845 yogo sapphire Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of method with medical stone bucket High Density Cultivation Perna viridis larva, belongs to aquaculture field.Technological core be first in Distilled water bucket with the bait China box-like algae of Magnetized Seawater culture Perna viridis larva, the double tail algaes of Bu Shi, wing root pipe algaes, after with Magnetized Seawater culture Perna viridis larva and feed the double tail algaes of Chinese box-like algae, Bu Shi, wing root pipe algaes in medical stone bucket.My research and test result indicates that, compared with conventional art, micro algae culturing liquid scientific formulation of the present invention is reasonable, and algal grown is fast, yield is high, not easy to pollute, easy to operate, equipment is simple, with medical stone bucket and Magnetized Seawater culture Perna viridis larva and feed the double tail algaes of Chinese box-like algae, Bu Shi, wing root pipe algae, larval growth is fast, physical strong, active, and survival rate improves 120%.
Description
Technical field
The invention belongs to aquaculture field, more particularly to it is a kind of with medical stone bucket High Density Cultivation Perna viridis larva
Method.
Background technology
Medical stone be it is a kind of to biological nontoxic, complex mineral that is harmless and having certain bioactivity and medicinal rock, mainly
Chemical composition is inorganic silicon-aluminum hydrochlorate, including SiO2、Al2O3、Fe2O3、FeO、MgO、CaO、K2O、Na2O、TiO2、P2O5、MnO
Deng, containing K, Na, Ca, Mg, Cu, Mo needed for animal etc. trace element and rare earth element, about 58 kinds.Medical stone main mine material is
Volcanic rock.Before 50000000-7,550 ten thousand years, the lava that volcanic eruption goes out is embedded in underground, acts on, melts by scabland high humidity is hot
Rock is changed into acidic materials, produces pressure effect by diastrophism, ultimately forms medical stone.Medical stone is a kind of special nonmetallic
Mineral resources, are similar to plaque-like, yellow or white, quite like wheat.Ming Dynasty's physician's Li Shizhen (1518-1593 A.D.) exists《Compendium of Materia Medica》Described in：" shape is such as
A poly- wheat meal is held, there is grain such as beans such as rice, its color is yellowish-white." medical stone is so gain the name.Medical stone powder enters Chinese medicine, the water soaked
It is drinkable, it can also take a shower, have body-building and a drug effect of promoting longevity.Medical stone passes to the Japanese good name for obtaining " healthy remedies ".Medical stone
Be soaked in water nearly 60 kinds of elements and mineral matter necessary to human body such as leachable potassium, sodium, calcium, magnesium, silicon, violent, titanium, phosphorus, up to ore deposit
Spring standard, body immunity can be strengthened for a long time by drinking；At the same time medical stone have to the harmful elements such as cadmium, lead and bacterial mass compared with
Strong suction-operated, to Escherichia coli adsorption rate more than 95%.My research shows that medical stone is to Perna viridis larva and China
The marine microalgae growths such as the double tail algaes of box-like algae, Bu Shi, wing root pipe algae are obviously promoted effect.
The green mussel clam of Penera viridis popular name, mussel, are bivalve, are distributed mainly on new add
Slope, Malaysia, Indonesia, South Korea, China's Mainland, Taiwan are coastal, coastal waters intertidal zone to shallow sea bottom are often lived in, except seashore rock reef
Outside, also it is found in floating log, hull bottom etc..Shell is about 100mm, the wide 36mm of shell.Hull shape approximation Trachyostracous mussel, but chitin is planted earlier above
It is thin, it is solid.Shell front end is tapering, the wide circle in rear end.Shell surface is smooth, is usually emerald green or green and brown color, and the young is bright in luster.Inner face
White, has gloss, unmatched adductor scar.Left housing has 2 hinge tooths, right 1, shell.This shellfish individual is big, and meat flavour is delicious, is important warp
Ji shellfish.Shellfish meat or a kind of medicinal material,《Medical Animals In China will》Referred to as " mussel ", beneficial the moon are enriched blood, and QI invigorating replenishing essence, the goitre that disappears dissipates
Knot, stop dysentery；Cure mainly insufficiency of vital energy and blood, consumptive disease is become thin, impotence caused by all empty, kidney deficiency of anaemia is not lifted, soreness and weakness of waist and knees, pain, goitre gas,
The disease of the sinking of qi of middle-jiao, protracted dysentery and diarrhea.
In Perna viridis production, provide the bait of amount foot of fine quality for larva and ensure that higher larva survival rate is whole
The key link of production procedure.On Perna viridis nursery and larvae cultivation method, past Lee discloses a kind of thick shell after Ji etc.
Mussel floating larvae foundation bait formula (application number CN201310569842), by part of water body containing Micro Algae of equal cell density
Number contains：65 to 72 parts of water body containing Isochrysis galbana, containing Nannochloropsis oculata or chlorella or the two arbitrary proportion mixed water body 8 to 16
Part, 16 to 24 parts of water body containing diatom.Tool nutrition is comprehensive, attractant is good, feed coefficient is low, of low cost, culture facilitates advantage, but
Easy to pollute, production is unstable.In addition care for loyal flag etc. and invent a kind of method (application number of Trachyostracous mussel artificial breeding
CN201110139046), the artificial nursery stock growing method for thick-shell mussel which provides, includes larval culture step, the step water temperature
For 14-21 DEG C, salinity 25-28, daily morning changes water 1 time with siphonage or bottom of pond drainage, and total quantity of exchanged water is gradual from 30%
Increase to 50%；Using algae as bait, day bait throwing in 2 times, i.e., feed 1 time after changing water morning, at dusk according to cultivation Measures of Algae in Water Body
Density, supplement feed 1 time, to keep algae density to require.The artificial nursery stock growing method for thick-shell mussel that the invention provides, to existing
Technology has made further research, makes the water temperature in wherein larval culture step require to decline, and by preferred other specification, make training
It is more reasonable to educate technique, compared with prior art, the invention energy consumption is low, the young seedling of gained is strong but troublesome in poeration, technical requirements
It is high.Chang Kangmei etc. discloses a kind of artificial nursery stock growing method for thick-shell mussel (application number CN200710156048).The invention includes parent
The nursery steps such as shellfish selects, close shellfish hot housing, the stimulation by running water that dries in the shade is hastened parturition, spawning and hatching, larval culture, juvenile mollusk are cultivated.Should
Invention can make Trachyostracous mussel realize natural spawning and obtain high-quality essence, ovum, and it is high with incubation rate to be fertilized, and can realize batch production
Full artificial culture Trachyostracous mussel seed, smoothly cultivates healthy, high-quality Trachyostracous mussel juvenile mollusk, and seedling raising process it is workable,
Consistent, shortcoming is technological process complexity, is spent more.
It is young with medical stone bucket High Density Cultivation Perna viridis the invention discloses one kind in order to overcome the shortcomings of current technology
The method of worm, technological core are first in Distilled water bucket with the bait of Magnetized Seawater culture Perna viridis larva -- Chinese box-like
The double tail algae of algae, Bu Shi, wing root pipe algaes, after with Magnetized Seawater culture Perna viridis larva and feed Chinese box-like in medical stone bucket
The double tail algaes of algae, Bu Shi, wing root pipe algae.My research and test result indicates that, compared with conventional art, micro algae culturing liquid of the present invention
Scientific formulation is reasonable, and algal grown is fast, and yield is high, and not easy to pollute, easy to operate, equipment is simple, with medical stone bucket and magnetization sea
Aquaponic Perna viridis larva simultaneously feeds the double tail algaes of Chinese box-like algae, Bu Shi, wing root pipe algae, and larval growth is fast, physical strong, into
Motility rate improves 120%.
The content of the invention
It is young with medical stone bucket High Density Cultivation Perna viridis the present invention provides one kind in order to overcome the shortcomings of current technology
The method of worm, method are as follows：
The preparation of three kinds of bait micro-algaes of step 1 (Chinese box-like algae, the double tail algaes of Bu Shi, wing root pipe algae) nutrient solution
Micro algae culturing liquid component (ratio of weight and number)：1-1.2 parts of human urine, NH40.1-0.2 parts of Cl, KH2PO4 0.02-
0.04 part, NaHCO30.8-1.2 parts, MgSO4·7H20.03-0.05 parts of O, (NH4)2SO40.04-0.08 parts, CaCl2·
2H20.8-1.2 parts of 0.05-0.08 parts of O, NaCl, Na2SiO30.04-0.06 parts, CH3COONa, 0.03-0.07 parts, FeSO4
0.06-0.09 parts, MnSO4·7H20.003-0.006 parts of O, CoCl2·6H20.007-0.009 parts of O, NiCl2·6H2O
0.003-0.006 parts, CH3CH20.03-0.06 parts of COONa, 0.2-0.4 parts of urea, 0.5-0.8 parts of titanium solution, thiamine hydrochloride
0.0001-0.002 parts, 0.2-0.5 parts of medical stone powder, 1000 parts of Magnetized Seawater.Three kinds of bait micro-algaes (Chinese box-like algae, Bu Shi
Double tail algaes, wing root pipe algae) nutrient solution component prescription is identical.
The culture of three kinds of microalgaes of step 2
3 10000 milliliters of Distilled water buckets washed are taken, each bottle adds nutrient solution 5000-6000 described in step 1
Milliliter, shakes up, take growth it is vigorous, in exponential phase of growth, pollution-free, purebred Chinese box-like algae, the double tail algaes of Bu Shi, wing root pipe
Algae is inoculated with respectively successively, carries out monoculture.Inoculum density is Chinese box-like algae ten thousand cells/mls of 2-3, the double tails of Bu Shi
Ten thousand cells/mls of algae 4-6, wing root pipe algae rotary strainer algae ten thousand cells/mls of 3.5-5, shake up, put this Distilled water bucket into microalgae
Culturing room is cultivated.Clogged at bung hole with a rubber stopper, 2 holes are stamped on plug, a diameter of 5 millimeters of hole, are inserted into length
2 glass tubes not waited, 5 millimeters of glass tube external diameter, a longer root long degree is 70-80 centimetres, to inflate, shorter one
For exhaust pipe, length is 8-10 centimetres, and glass is exposed at both ends.Long glass bottom of the tube insertion is more soft close to bucket bottom, upper end use
Soft plastics or emulsion tube are connected with air compressor machine, are filled with mixed gas, and each bucket aeration quantity is 3-5 liters/min, mixed gas into
It is divided into VAir∶VOzone∶VPure carbon dioxide∶VPure ammonia=98%: 1%: 0.8%: 0.2% (volume ratio), continuous uninterrupted inflation.Microdisk electrode
Room conditioning temperature control, CFL lighting, co-cultures 9 days, preceding 3 days temperature are set to 21-22 DEG C, intensity of illumination 3000-
4000lux, the 4-6 days temperature are set to 23-25 DEG C, intensity of illumination 6000-7000lux, and is set to 27-29 DEG C and 8000- in 7-9 days
10000lux, 800 milliliters of fresh medium described in the 6th day each bucket addition step 1, with the consumption of extra-nutrition salt, the 7th day
Each 500 milliliters of distilled water of bottle addition, to supplement the loss of water caused by inflation and dilute algae solution, by the 9th day, Chinese box-like
Algae density is up to 900,000-120 ten thousand cells/mls, and the double tail algae density of Bu Shi are up to 500,000-75 ten thousand cells/mls, wing root pipe algae
Up to 650,000-85 ten thousand cells/mls, stop culture, prepare to feed Perna viridis larva with this algae solution.
The culture of step 3 Perna viridis larva
1 cylindrical medical stone bucket is taken, 70-80 centimetres of diameter is 65-75 centimetres high, and 2-3 centimetres of bucket thickness, emerald is made a gift of
Shellfish D-shaped larva, which is placed in this bucket, to be cultivated, and opening culture (i.e. bucket is without capping), culture is Magnetized Seawater with water, adds Magnetized Seawater
To 0.40-45 meters of the depth of water, indoor culture, air-conditioning temperature control, CFL lighting, adjusts the temperature to 23-26 DEG C, illumination 2000-
3000lux, Perna viridis D-shaped larva breeding density are 5-7/milliliter, and Chinese box-like algae is fed daily by each larva
10000-15000,2000-3000, Bu Shi double tail algaes, the amount that wing root pipe algae is 6000-8000 feeds 3 kinds of algae solutions, divides in morning
Evening feeds three times, and when small per minor tick 8, later with larval growth, body length, which often increases, to be twice, and daily ration, feeding quantity, which also increases, to be twice, together
When uninterruptedly inflated with air compressor machine, be filled with mixed gas, mixed gas composition VAir∶VOzone∶VPure carbon dioxide∶VPurity oxygen=97%: 2%
: 0.5%: 0.5%；Inflate pipe end and connect a gas exhausting stone, aeration quantity is 8-12 liters/min.When paedomorphosis to period of metamorphosis,
There is 40% young eyespot occur, you can to launch attachment base, with the polyethylene mesh sheet 6-12 pieces of 20 mesh after disinfection, be divided into 3 beams and hang
Hang over and be used as attachment base in medical stone bucket, change water once with siphon tube method daily, quantity of exchanged water 1/4-1/3, with larval growth,
Quantity of exchanged water also gradually increases, to shell top larva later stage quantity of exchanged water up to 80%；Silk cover filtering, stop when changing water with 120 mesh, avoid
Larva escapes with current；Culture 28-32 days, when juvenile mollusk shell is high 0.25 centimetre reachable, the seedling in mesh sheet is scrubbed, you can move
Enter ocean culture or sale.
For the disinfectant that the polyethylene mesh sheet uses for Chinese herbal medicine composite disinfectant, sterilization method is immersion 20 minutes.
Wherein Chinese herbal medicine composite disinfectant constituent (ratio of weight and number)：Red 2-4 parts of dish of the back of the body, 6-9 parts of cordate houttuynia, radix bupleuri
5-8 parts, 7-12 parts of Longtube Ground Ivy Herb, 10-15 parts of the root bark of shaggy-fruited dittany, 7-12 parts of rhizoma atractylodis, 8-15 parts of the fruit of summer cypress is 5-7 parts windproof, 3-6 parts of peppermint,
0.5-0.9 parts of Radix Isatidis, 10-14 parts of the root of Dahurain angelica, 5-8 parts of Herba Andrographitis, 2-6 parts of sea-buckthorn, 1-3 parts of Phellinus.
Beneficial outcomes
It is young with medical stone bucket High Density Cultivation Perna viridis the present invention provides one kind in order to overcome the shortcomings of current technology
The method of worm, technological core are first in Distilled water bucket with the bait of Magnetized Seawater culture Perna viridis larva -- Chinese box-like
The double tail algae of algae, Bu Shi, wing root pipe algaes, after with Magnetized Seawater culture Perna viridis larva and feed Chinese box-like in medical stone bucket
The double tail algaes of algae, Bu Shi, wing root pipe algae.My research and test result indicates that, compared with conventional art, micro algae culturing liquid of the present invention
Scientific formulation is reasonable, and algal grown is fast, and yield is high, and not easy to pollute, easy to operate, equipment is simple, with medical stone bucket and magnetization sea
Aquaponic Perna viridis larva simultaneously feeds the double tail algaes of Chinese box-like algae, Bu Shi, wing root pipe algae, and larval growth is fast, physical strong, into
Motility rate improves 120%.
Brief description of the drawings
Fig. 1 Distilled water bucket culture bait micro-algae figures；
Wherein arrow is signified moves towards for charge air conditioning；The Chinese box-like algae of wherein A barrels of culture, the B barrels of double tail algaes of culture Bu Shi, C
Bucket culture wing root pipe algae；
Embodiment
A kind of method with medical stone bucket High Density Cultivation Perna viridis larva, method are as follows：
The preparation of three kinds of bait micro-algaes of step 1 (Chinese box-like algae, the double tail algaes of Bu Shi, wing root pipe algae) nutrient solution
Micro algae culturing liquid component (ratio of weight and number)：1-1.2 parts of human urine, NH40.1-0.2 parts of Cl, KH2PO4 0.02-
0.04 part, NaHCO30.8-1.2 parts, MgSO4·7H20.03-0.05 parts of O, (NH4)2SO40.04-0.08 parts, CaCl2·
2H20.8-1.2 parts of 0.05-0.08 parts of O, NaCl, Na2SiO30.04-0.06 parts, CH3COONa, 0.03-0.07 parts, FeSO4
0.06-0.09 parts, MnSO4·7H20.003-0.006 parts of O, CoCl2·6H20.007-0.009 parts of O, NiCl2·6H2O
0.003-0.006 parts, CH3CH20.03-0.06 parts of COONa, 0.2-0.4 parts of urea, 0.5-0.8 parts of titanium solution, thiamine hydrochloride
0.0001-0.002 parts, 0.2-0.5 parts of medical stone powder, 1000 parts of Magnetized Seawater.Three kinds of bait micro-algaes (Chinese box-like algae, Bu Shi
Double tail algaes, wing root pipe algae) nutrient solution component prescription is identical.
The culture of three kinds of microalgaes of step 2
3 10000 milliliters of Distilled water buckets washed are taken, each bottle adds nutrient solution 5000-6000 described in step 1
Milliliter, shakes up, take growth it is vigorous, in exponential phase of growth, pollution-free, purebred Chinese box-like algae, the double tail algaes of Bu Shi, wing root pipe
Algae is inoculated with respectively successively, carries out monoculture.Inoculum density is Chinese box-like algae ten thousand cells/mls of 2-3, the double tails of Bu Shi
Ten thousand cells/mls of algae 4-6, wing root pipe algae rotary strainer algae ten thousand cells/mls of 3.5-5, shake up, put this Distilled water bucket into microalgae
Culturing room is cultivated.Clogged at bung hole with a rubber stopper, 2 holes are stamped on plug, a diameter of 5 millimeters of hole, are inserted into length
2 glass tubes not waited, 5 millimeters of glass tube external diameter, a longer root long degree is 70-80 centimetres, to inflate, shorter one
For exhaust pipe, length is 8-10 centimetres, and glass is exposed at both ends.Long glass bottom of the tube insertion is more soft close to bucket bottom, upper end use
Soft plastics or emulsion tube are connected with air compressor machine, are filled with mixed gas, and each bucket aeration quantity is 3-5 liters/min, mixed gas into
It is divided into VAir∶VOzone∶VPure carbon dioxide∶VPure ammonia=98%: 1%: 0.8%: 0.2% (volume ratio), continuous uninterrupted inflation.Microdisk electrode
Room conditioning temperature control, CFL lighting, co-cultures 9 days, preceding 3 days temperature are set to 21-22 DEG C, intensity of illumination 3000-
4000lux, the 4-6 days temperature are set to 23-25 DEG C, intensity of illumination 6000-7000lux, and is set to 27-29 DEG C and 8000- in 7-9 days
10000lux, 800 milliliters of fresh medium described in the 6th day each bucket addition step 1, with the consumption of extra-nutrition salt, the 7th day
Each 500 milliliters of distilled water of bottle addition, to supplement the loss of water caused by inflation and dilute algae solution, by the 9th day, Chinese box-like
Algae density is up to 900,000-120 ten thousand cells/mls, and the double tail algae density of Bu Shi are up to 500,000-75 ten thousand cells/mls, wing root pipe algae
Up to 650,000-85 ten thousand cells/mls, stop culture, prepare to feed Perna viridis larva with this algae solution.
The culture of step 3 Perna viridis larva
1 cylindrical medical stone bucket is taken, 70-80 centimetres of diameter is 65-75 centimetres high, and 2-3 centimetres of bucket thickness, emerald is made a gift of
Shellfish D-shaped larva, which is placed in this bucket, to be cultivated, and opening culture (i.e. bucket is without capping), culture is Magnetized Seawater with water, adds Magnetized Seawater
To 0.40-45 meters of the depth of water, indoor culture, air-conditioning temperature control, CFL lighting, adjusts the temperature to 23-26 DEG C, illumination 2000-
3000lux, Perna viridis D-shaped larva breeding density are 5-7/milliliter, and Chinese box-like algae is fed daily by each larva
10000-15000,2000-3000, Bu Shi double tail algaes, the amount that wing root pipe algae is 6000-8000 feeds 3 kinds of algae solutions, divides in morning
Evening feeds three times, and when small per minor tick 8, later with larval growth, body length, which often increases, to be twice, and daily ration, feeding quantity, which also increases, to be twice, together
When uninterruptedly inflated with air compressor machine, be filled with mixed gas, mixed gas composition VAir∶VOzone∶VPure carbon dioxide∶VPurity oxygen=97%: 2%
: 0.5%: 0.5%；Inflate pipe end and connect a gas exhausting stone, aeration quantity is 8-12 liters/min.When paedomorphosis to period of metamorphosis,
There is 40% young eyespot occur, you can to launch attachment base, with the polyethylene mesh sheet 6-12 pieces of 20 mesh after disinfection, be divided into 3 beams and hang
Hang over and be used as attachment base in medical stone bucket, change water once with siphon tube method daily, quantity of exchanged water 1/4-1/3, with larval growth,
Quantity of exchanged water also gradually increases, to shell top larva later stage quantity of exchanged water up to 80%；Silk cover filtering, stop when changing water with 120 mesh, avoid
Larva escapes with current；Culture 28-32 days, when juvenile mollusk shell is high 0.25 centimetre reachable, the seedling in mesh sheet is scrubbed, you can move
Enter ocean culture or sale.
The disinfectant that the polyethylene mesh sheet uses is 0.4-0.7 parts of Chinese herbal medicine composite disinfectant.
Wherein Chinese herbal medicine composite disinfectant constituent (ratio of weight and number)：Red 2-4 parts of dish of the back of the body, 6-9 parts of cordate houttuynia, radix bupleuri
5-8 parts, 7-12 parts of Longtube Ground Ivy Herb, 10-15 parts of the root bark of shaggy-fruited dittany, 7-12 parts of rhizoma atractylodis, 8-15 parts of the fruit of summer cypress is 5-7 parts windproof, 3-6 parts of peppermint,
0.5-0.9 parts of Radix Isatidis, 10-14 parts of the root of Dahurain angelica, 5-8 parts of Herba Andrographitis, 2-6 parts of sea-buckthorn, 1-3 parts of Phellinus.
NH described in step 14Cl, KH2PO4, NaHCO3, MgSO4·7H2O, (NH4)2SO4, CaCl2·2H2O, NaCl,
Na2SiO3, CH3COONa, FeSO4, MnSO4·7H2O, CoCl2·6H2O, NiCl2·6H2O, CH3CH2COONa chemicals is pure
Degree is both needed to the pure rank of chemistry；But urea can be common agrochemical rank；
Human urine preparation method described in step 1：Urine is taken, when spontaneous fermentation 72 is small；One 3000 milliliters of conical flasks are taken, are added
Enter 1500 milliliters of the urine fermented, heat, boil 4-5 minutes, cool down, it is spare；
Titanium solution preparation method described in step 1 (weight ratio)：0.7366 part of titanium oxalate, 100 parts of distilled water are dissolved in
In, 5 parts of ammonium hydroxide are added, alkalescence is allowed into, precipitates, will be precipitated and concentrated with centrifugal process, with the sulphur of 20 parts of 0.05mol/L
Acid dissolves to obtain the final product；
Three kinds of microalgaes described in step 2 are Chinese box-like algaes, the double tail algaes of Bu Shi, wing root pipe algae；
Inoculation is purified (this area routine side in advance with Chinese box-like algae, the double tail algaes of Bu Shi, wing root pipe algae in step 2
Method, if realize purification, such as Michaelis extraction method), algae is purebred and be in eugonic finger during ensuring inoculation
Number growth period；
Step 1 and the 3 Magnetized Seawater preparation methods：Seawater is taken, absorbent cotton filtering, heating, boils 3-5 minutes, cools down,
It is magnetized water after flowing through water magnetizing apptss；Water magnetizing apptss magnetic induction intensity is 0.3T, flow 2-3t/h, flow velocity 0.1-
0.2m/s；
Various nutritive salt will be added by the order provided in formula in step 1, to avoid chemical reaction；
In step 2 rubber stopper used, glass tube be both needed to wash be placed on dried in 65-75 DEG C of baking oven after use；
Medical stone powder preparation method described in step 1：Medical stone is taken, pulverizer is crushed to floury, and particle can pass through 100-
120 mesh sieve thin,tough silk；
Seeded process described in step 2 need to carry out on aseptic operating platform；
Polyethylene mesh sheet sterilization method is used described in step 3：Mesh sheet is washed, after be placed in 5% malachite green solution
When (weight ratio) immersion 12 is small, after rinsed well with boiling refrigerated sea water；
Attachment base described in step 3, if not having polyethylene mesh sheet, can be replaced with coir rope, and usage is identical with dosage, but poly- second
Cultivating survival rate is high after the attachment of alkene mesh sheet and growth is fast, output of seedling higher, it is therefore proposed that most handy polythene net piece spatfall；
Water temperature must keep stable during larva culture described in step 3, and temperature difference per day is no more than 1 DEG C, otherwise easily causes the young
Sink to the bottom death.
Finally it should be noted that：The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
The present invention is described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that：It still may be used
To modify to the technical solution described in previous embodiment, or equivalent substitution is carried out to which part technical characteristic；And
These modifications are replaced, and the essence of appropriate technical solution is departed from the spirit and model of technical solution of the embodiment of the present invention
Enclose.
Claims (1)
- A kind of 1. method with medical stone bucket High Density Cultivation Perna viridis larva, it is characterised in that step is as follows：The preparation of three kinds of bait micro-algae nutrient solutions of step 1；Three kinds of bait micro-algaes are Chinese box-like algae and the double tail algaes of Bu Shi and wing root pipe algae；Micro algae culturing liquid component, it is as follows according to ratio of weight and number：1-1.2 parts of human urine, NH40.1-0.2 parts of Cl, KH2PO40.02- 0.04 part, NaHCO30.8-1.2 parts, MgSO4·7H20.03-0.05 parts of O, (NH4)2SO40.04-0.08 parts, CaCl2·2H2O 0.05-0.08 parts, 0.8-1.2 parts of NaCl, Na2SiO30.04-0.06 parts, CH3COONa, 0.03-0.07 parts, FeSO40.06- 0.09 part, MnSO4·7H20.003-0.006 parts of O, CoCl2·6H20.007-0.009 parts of O, NiCl2·6H2O 0.003- 0.006 part, CH3CH20.03-0.06 parts of COONa, 0.2-0.4 parts of urea, 0.5-0.8 parts of titanium solution, thiamine hydrochloride 0.0001-0.002 parts, 0.2-0.5 parts of medical stone powder, 1000 parts of Magnetized Seawater；Three kinds of bait micro-algaes " Chinese box-like algae, Bu Shi Double tail algaes, wing root pipe algae " nutrient solution component prescription is identical；The culture of three kinds of microalgaes of step 2；3 10000 milliliters of Distilled water buckets washed are taken, each bucket adds nutrient solution 5000-6000 millis described in step 1 Rise, shake up, take growth it is vigorous, in exponential phase of growth, pollution-free, purebred Chinese box-like algae, the double tail algaes of Bu Shi, wing root pipe algae It is inoculated with respectively successively, carries out monoculture；Inoculum density is Chinese box-like algae ten thousand cells/mls of 2-3, the double tail algaes of Bu Shi Ten thousand cells/mls of 4-6, wing root pipe algae rotary strainer algae ten thousand cells/mls of 3.5-5, shake up, and put this Distilled water bucket into microalgae training Cultivated foster room；Clogged at bung hole with a rubber stopper, 2 holes are stamped on plug, a diameter of 5 millimeters of hole, insertion length is not Deng 2 glass tubes, 5 millimeters of glass tube external diameter, a longer root long degree be 70-80 centimetres, to inflate, shorter one is Exhaust pipe, length are 8-10 centimetres；Long glass bottom of the tube is inserted into close to bucket bottom, upper end relatively soft plastics or emulsion tube and sky Press is connected, and is filled with mixed gas, and each bucket aeration quantity is 3-5 liters/min, mixed gas composition VAir∶VOzone∶VPure carbon dioxide∶ VPure ammonia=98%: 1%: 0.8%: 0.2% (volume ratio), continuous uninterrupted inflation；Microdisk electrode room conditioning temperature control, energy-saving lamp Illumination, co-cultures 9 days, preceding 3 days temperature are set to 21-22 DEG C, and intensity of illumination 3000-4000lux, temperature is set to 23- within the 4-6 days 25 DEG C, intensity of illumination 6000-7000lux, the is set to 27-29 DEG C and 8000-10000lux for 7-9 days, the 6th day each bucket addition step 800 milliliters of nutrient solution in rapid 1, with the consumption of extra-nutrition salt, the 7th day each 500 milliliters of distilled water of bucket addition, to supplement because filling Loss of water caused by gas simultaneously dilutes algae solution, and by the 9th day, Chinese box-like algae density was up to 900,000-120 ten thousand cells/mls, cloth The double tail algae density of family name stop culture, standard up to 500,000-75 ten thousand cells/mls, wing root pipe algae up to 650,000-85 ten thousand cells/mls This spare algae solution feeds Perna viridis larva；The culture of step 3 Perna viridis larva；1 cylindrical medical stone bucket is taken, 70-80 centimetres of diameter is 65-75 centimetres high, 2-3 centimetres of bucket thickness, by Perna viridis D-shaped Larva is placed in this bucket and cultivates, and opening culture (i.e. bucket is without capping), culture is Magnetized Seawater with water, adds Magnetized Seawater to the depth of water 0.40-45 meters, indoor culture, air-conditioning temperature control, CFL lighting, adjusts the temperature to 23-26 DEG C, illumination 2000-3000lux, bird with red feathers Emerald green mussel D-shaped larva breeding density is 5-7/milliliter, and Chinese box-like algae 10000-15000 are fed daily by each larva, 2000-3000, Bu Shi double tail algaes, the amount that wing root pipe algae is 6000-8000 feeds 3 kinds of algae solutions, divides and feeds three times in the morning, afternoon and evening, often When minor tick 8 is small, later with the growth of larva, body length, which often increases, to be twice, and daily ration, feeding quantity, which also increases, to be twice, while with air compressor machine not Interruption inflation, is filled with mixed gas, mixed gas composition VAir∶VOzone∶VPure carbon dioxide∶VPurity oxygen=97%: 2%: 0.5%: 0.5%；Inflate pipe end and connect a gas exhausting stone, aeration quantity is 8-12 liters/min；When paedomorphosis to period of metamorphosis, there is 40% There is eyespot in the young, you can launches attachment base, with the polyethylene mesh sheet 6-12 pieces of 20 mesh after disinfection, is divided into the hanging of 3 beams in wheat It is used as attachment base in meal stone bucket, changes water once with siphon tube method daily, quantity of exchanged water 1/4-1/3, with larval growth, quantity of exchanged water Also gradually increase, to shell top larva later stage quantity of exchanged water up to 80%；When changing water with the silk cover filterings of 100 mesh, stop, avoid larva with Current are escaped；Culture 28-32 days, when juvenile mollusk shell is high 0.25 centimetre reachable, the seedling in mesh sheet is scrubbed, can move into ocean Culture or sale.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610127343.3A CN105724293B (en) | 2016-03-08 | 2016-03-08 | A kind of method with medical stone bucket High Density Cultivation Perna viridis larva |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610127343.3A CN105724293B (en) | 2016-03-08 | 2016-03-08 | A kind of method with medical stone bucket High Density Cultivation Perna viridis larva |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105724293A CN105724293A (en) | 2016-07-06 |
| CN105724293B true CN105724293B (en) | 2018-04-17 |
Family
ID=56249216
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610127343.3A Active CN105724293B (en) | 2016-03-08 | 2016-03-08 | A kind of method with medical stone bucket High Density Cultivation Perna viridis larva |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105724293B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113243341B (en) * | 2021-06-22 | 2022-06-03 | 中国人民解放军国防科技大学 | Breeding device, breeding method, antifouling test system and antifouling test method |
| CN115968708B (en) * | 2022-12-19 | 2024-06-18 | 青岛市农业科学研究院 | Protective device for fruit tree seedlings |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1539269A (en) * | 2003-08-22 | 2004-10-27 | 中国科学院海洋研究所 | Method for cleansing shellfish lived and buried on tidal flat |
| CN1561705A (en) * | 2004-03-14 | 2005-01-12 | 中国水产科学研究院黄海水产研究所 | High density cultivating device and cultivating method for bivalve juvenile mollusk |
| CN101317549A (en) * | 2007-10-10 | 2008-12-10 | 浙江海洋学院 | Artificial nursery stock growing method for thick-shell mussel |
| CN103805515A (en) * | 2014-03-04 | 2014-05-21 | 临沂大学 | Culture medium and culture method for Dicrateria zhanjiangensis |
| CN103834570A (en) * | 2014-03-10 | 2014-06-04 | 临沂大学 | Culture medium and culture method for mixing culturing of phaeodactylum tricornutum bohlin and nitzschia closterium |
| CN103966102A (en) * | 2014-05-26 | 2014-08-06 | 临沂大学 | Culture medium for culturing Chlamydomonas reinhardtii by urea plant wastewater and culture method of Chlamydomonas reinhardtii |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004057206A (en) * | 2002-06-07 | 2004-02-26 | Ikeda Shokken Kk | Feed |
-
2016
- 2016-03-08 CN CN201610127343.3A patent/CN105724293B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1539269A (en) * | 2003-08-22 | 2004-10-27 | 中国科学院海洋研究所 | Method for cleansing shellfish lived and buried on tidal flat |
| CN1561705A (en) * | 2004-03-14 | 2005-01-12 | 中国水产科学研究院黄海水产研究所 | High density cultivating device and cultivating method for bivalve juvenile mollusk |
| CN101317549A (en) * | 2007-10-10 | 2008-12-10 | 浙江海洋学院 | Artificial nursery stock growing method for thick-shell mussel |
| CN103805515A (en) * | 2014-03-04 | 2014-05-21 | 临沂大学 | Culture medium and culture method for Dicrateria zhanjiangensis |
| CN103834570A (en) * | 2014-03-10 | 2014-06-04 | 临沂大学 | Culture medium and culture method for mixing culturing of phaeodactylum tricornutum bohlin and nitzschia closterium |
| CN103966102A (en) * | 2014-05-26 | 2014-08-06 | 临沂大学 | Culture medium for culturing Chlamydomonas reinhardtii by urea plant wastewater and culture method of Chlamydomonas reinhardtii |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105724293A (en) | 2016-07-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103430877A (en) | Comprehensive and ecological river crab pond culturing method | |
| CN104719669B (en) | A kind of making of Chlamys farreri young bait and feeding method | |
| CN103563803B (en) | A kind of cultural method reducing stichopus japonicus damage by disease and insect and occur | |
| CN103283641B (en) | A kind of hybridized prussian carp is at coastal saline-alkali water pond high-yield cultivation method | |
| CN103936174B (en) | Biological agent for preventing and treating mosses in water body and preparation method of biological agent | |
| CN105123563A (en) | Mixed culture method for penaeus vannamei and macrobrachium rosenbergii | |
| CN104593262A (en) | Series cultivation and rapid collection method for marine microalgae | |
| CN104212718A (en) | Culture medium and culture method for culturing chlorella pyrenoidosa by sheep manure leach liquor | |
| CN104799051B (en) | A kind of preparation method of razor clam ecological bait | |
| CN105010807A (en) | Living fly maggot liquid protein leech culturing feed and preparation method thereof | |
| CN102017905B (en) | Method for breeding pinaeus monodon fries | |
| CN105724293B (en) | A kind of method with medical stone bucket High Density Cultivation Perna viridis larva | |
| CN107691304A (en) | A kind of large yellow croaker good quality and high output cultural method | |
| CN106614171B (en) | A kind of integrated breeding method of efficient crab seedling rearing | |
| CN106819655A (en) | A kind of fermentation of Chinese herbal medicine additive for improving oyster seed yield and its preparation application method | |
| CN104719207B (en) | A kind of method of use waste or used plastics bucket culture brachionus plicatilis | |
| CN103563806B (en) | Cultivation method capable of reducing stichopus japonicus disease and pest injuries with corrosion inhibition nanometer materials | |
| CN105475195B (en) | A method of with the nuisanceless culture haliotis diversicolor Reeve juvenile mollusk of waste gasoline bucket | |
| CN102017910B (en) | Energy-saving and emission-reducing culture method of turbot | |
| CN107969357A (en) | A kind of high-efficient method for cultivating of fish fry | |
| CN109006605B (en) | Freshwater ecological breeding method for penaeus vannamei boone | |
| CN106665437A (en) | Chinese herbal medicine additive for improving cultivation survival rate of maternal oysters and its preparation method and use method | |
| CN106561507A (en) | Method for breeding tilapia in rice field | |
| CN1168381C (en) | Ecological breeding and ecological culturing method for Chinese fine hair chela crab | |
| CN110250047A (en) | A kind of method of industrial aquaculture threadfin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201209 Address after: 331300 salt chemical industry city, Xin Gang county, Ji'an, Jiangxi Patentee after: Jiangxi Xinzhen Technology Co.,Ltd. Address before: Room 1602, B1 # Building, Lijinxiu Jiangnan (West District), east of Quyun Road, north of Yinhe No. 2 Road, Suzhou City, Anhui Province Patentee before: Anhui Huiteng Intellectual Property Co.,Ltd. Effective date of registration: 20201209 Address after: Room 1602, B1 # Building, Lijinxiu Jiangnan (West District), east of Quyun Road, north of Yinhe No. 2 Road, Suzhou City, Anhui Province Patentee after: Anhui Huiteng Intellectual Property Co.,Ltd. Address before: 276000 Linyi University, middle section of Shuangling Road, Linyi City, Shandong Province Patentee before: LINYI University |
|
| TR01 | Transfer of patent right |