CN105724293A - Method for high-density culture of perna viridis larvae through medical stone bucket - Google Patents
Method for high-density culture of perna viridis larvae through medical stone bucket Download PDFInfo
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- CN105724293A CN105724293A CN201610127343.3A CN201610127343A CN105724293A CN 105724293 A CN105724293 A CN 105724293A CN 201610127343 A CN201610127343 A CN 201610127343A CN 105724293 A CN105724293 A CN 105724293A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
Abstract
The invention discloses a method for high-density culture of perna viridis larvae through a medical stone bucket and belongs to the field of aquaculture.The method is technically characterized in that bait, namely, biddulphia sinensis, ditylum brightwellii and rhizosolenia alata, for perna viridis larvae is cultured with magnetized seawater in a distilled water bucket, and then the perna viridis larvae are cultured with the magnetized seawater in the medical stone bucket and fed with biddulphia sinensis, ditylum brightwellii and rhizosolenia alata.The study and experiment result in China shows that compared with a traditional technology, the microalgae culture solution is scientific and reasonable in formula, the algae grow fast, and are high in yield and not prone to be contaminated, operation is convenient, equipment is simple, the perna viridis larvae are cultured with the magnetized seawater in the medical stone bucket and fed with biddulphia sinensis, ditylum brightwellii and rhizosolenia alata, the larvae grow fast, healthy and strong, and lively, and the survival rate is increased by 120%.
Description
Technical field
The invention belongs to aquaculture field, a kind of method particularly relating to Maifanitum bucket High Density Cultivation Perna uiridis (Linnaeus) larva.
Background technology
Maifanitum be a kind of to biological nontoxic, harmless and have certain bioactive complex mineral and medicinal rock, primary chemical becomes
Dividing is inorganic silicon-aluminum hydrochlorate, including SiO2、Al2O3、Fe2O3、FeO、MgO、CaO、K2O、Na2O、TiO2、P2O5, MnO etc.,
Containing trace element and rare earth elements such as K, Na, Ca, Mg, Cu, the Mo needed for animal, about 58 kinds.Maifanitum main mine material
It it is volcanic rock.Before 50000000-7,550 ten thousand years, the lava that volcanic eruption goes out is embedded in underground, through scabland high humidity sweltering heat effect,
Lava becomes acidic materials, produces pressure effect by diastrophism, ultimately forms Maifanitum.Maifanitum is a kind of special non-gold
Belong to mineral resources, be similar to plaque-like, yellow or white, quite like wheat grain.Ming Dynasty's physician's Li Shizhen (1518-1593 A.D.) is described in Compendium of Material Medica:
" shape such as holds a poly-wheat meal, has grain such as bean such as rice, and its yellow skin is white." Maifanitum is so gaining the name.Medical stone powder enters Chinese medicine, leaching
The water steeped is drinkable, also can take a shower, and has body-building and life lengthening drug effect.Maifanitum passes to the good of Japanese " healthy YAOSHI "
Name.Maifanitum be soaked in water nearly 60 kinds of elements necessary to the human bodies such as leachable potassium, sodium, calcium, magnesium, silicon, violent, titanium, phosphorus and
Mineral, up to mineral water standard, long-term drink can enhancing human body immunity ability；Maifanitum is to the harmful element such as cadmium, lead simultaneously
And bacterial mass has stronger adsorption, to escherichia coli adsorption rate more than 95%.My research shows, Maifanitum is to Aeschna melanictera
Mytilus Edulis Larva and China box-like algae, Bu Shi double marine microalgae such as tail algae, wing root Guan Zao growth are obviously promoted effect.
Penera viridis popular name green mussel clam, Mytilus, for bivalve, be distributed mainly on Singapore,
Malaysia, Indonesia, Korea S, China's Mainland, Taiwan are coastal, often live in Intertidal zone, coastal waters at the bottom of shallow sea, except seashore cay
Outward, also it is found at floating log, hull bottom etc..Shell is about 100mm, shell width 36mm.Hull shape approximation Mytilus crassitesta Lischke, but chitin is relatively
Front kind is thin, solid.Shell front end is tapering, rear end width circle.Shell face is smooth, and the most emerald green or green and brown color, germling color is fresh
Gorgeous.Inner face white, has gloss, unmatched adductor scar.Left housing has 2 hinge tooths, 1, right shell.This shellfish is individual big, meat flavour
Deliciousness, is Important Economic shellfish.Shellfish meat or a kind of medical material, " Medical Animals In China will " referred to as " Mytilus ", tonifying YIN is mended
Blood, QI invigorating replenishing essence, the wart that disappears eliminating stagnation, dysentery relieving；Cure mainly insufficiency of vital energy and blood, asthenia is become thin, all void of anemia, suffer from a deficiency of the kidney caused sexual impotence not
Act, soreness of the waist and knees, pain, goiter, sinking of QI of middle-JIAO, the disease of chronic diarrhea chronic dysentery.
In Perna uiridis (Linnaeus) produces, provide the bait of amount of fine quality foot for larva and ensure that higher larva survival rate is whole production stream
The key link of journey.About Perna uiridis (Linnaeus) nursery and larvae cultivation method, a past Li Ji Ji etc. discloses a kind of Mytilus crassitesta Lischke and swims
Larva foundation bait formula (application number CN201310569842), is contained by the water body number containing Micro Algae of equal cell density:
Containing 65 to 72 parts of Isochrysis galbana water body, containing Nannochloropsis oceanica or chlorella or the two arbitrary proportion mixed water body 8 to 16 parts, contain
16 to 24 parts of diatom water body.Tool comprehensive nutrition, attractant is good, feed coefficient is low, with low cost, cultivate facilitate advantage, but
Easily pollute, produce instability.Additionally turn round and look at loyal flag etc. and invent the method (application number of a kind of Mytilus crassitesta Lischke artificial breeding
CN201110139046), the artificial nursery stock growing method for thick-shell mussel that this invention provides, comprise larval culture step, this step water temperature
For 14-21 DEG C, salinity is 25-28, morning every day siphonage or pond at the bottom of drainage change water 1 time, total quantity of exchanged water is from 30% gradually
Increase to 50%；With algae as bait, day bait throwing in 2 times, throw something and feed after i.e. changing water morning 1 time, at dusk according to cultivating algae in water body
The density of class, supplements and throws something and feeds 1 time, to keep algae density requirement.The artificial nursery stock growing method for thick-shell mussel that this invention provides, right
Prior art has made further research so that it is the water temperature in middle larval culture step requires to decline, and by preferably other parameters,
Making cultivating technique more reasonable, compared with prior art, this invention energy consumption is low, the young Seedling of gained is strong, but troublesome poeration, technology
Require height.Chang Kangmei etc. disclose a kind of artificial nursery stock growing method for thick-shell mussel (application number CN200710156048).This invention bag
Include parent shellfish select, the nursery step such as parent shellfish hot housing, the stimulation by running water that dries in the shade are hastened parturition, spawning and hatching, larval culture, juvenile mollusk cultivation
Suddenly.This invention can make Mytilus crassitesta Lischke realize natural spawning and obtain high-quality essence, ovum, and it is high with incubation rate to be fertilized, it is possible to realizes
Batch production full artificial culture Mytilus crassitesta Lischke seed, cultivates health, high-quality Mytilus crassitesta Lischke juvenile mollusk smoothly, and seedling raising process is operable
Property strong, consistent, shortcoming is that technological process is complicated, spends many.
In order to overcome the deficiency of current technology, the invention discloses the side of a kind of Maifanitum bucket High Density Cultivation Perna uiridis (Linnaeus) larva
Method, technological core is the bait first cultivating Perna uiridis (Linnaeus) larva in Distilled water bucket with Magnetized Seawater--China box-like algae, Bu Shi are double
Tail algae, wing root pipe algae, after to cultivate Perna uiridis (Linnaeus) larva China box-like algae of throwing something and feeding, Bu Shi with Magnetized Seawater in Maifanitum bucket double
Tail algae, wing root pipe algae.My research and test result indicate that, compared with conventional art, micro algae culturing liquid scientific formulation of the present invention
Rationally, algal grown is fast, and yield is high, is difficult to pollute, and easy to operate, equipment is simple, cultivates with Maifanitum bucket and Magnetized Seawater
Perna uiridis (Linnaeus) larva China box-like algae of throwing something and feeding, Bu Shi double tail algae, wing root pipe algae, larval growth is fast, and body constitution is healthy and strong, survival rate
Improve 120%.
Summary of the invention
In order to overcome the deficiency of current technology, the invention provides the side of a kind of Maifanitum bucket High Density Cultivation Perna uiridis (Linnaeus) larva
Method, method is as follows:
The preparation of three kinds of bait micro-algaes of step 1 (China box-like algae, the double tail algae of Bu Shi, wing root pipe algae) culture fluid
Micro algae culturing liquid composition (ratio of weight and number): Urina Hominis 1-1.2 part, NH4Cl 0.1-0.2 part, KH2PO4 0.02-0.04
Part, NaHCO30.8-1.2 part, MgSO4·7H2O 0.03-0.05 part, (NH4)2SO40.04-0.08 part, CaCl2·2H2O 0.05-0.08
Part, NaCl 0.8-1.2 part, Na2SiO30.04-0.06 part, CH3COONa, 0.03-0.07 part, FeSO40.06-0.09 part,
MnSO4·7H2O 0.003-0.006 part, CoCl2·6H2O 0.007-0.009 part, NiCl2·6H2O 0.003-0.006 part,
CH3CH2COONa 0.03-0.06 part, carbamide 0.2-0.4 part, titanium solution 0.5-0.8 part, thiamine hydrochloride 0.0001-0.002
Part, medical stone powder 0.2-0.5 part, Magnetized Seawater 1000 parts.Three kinds of bait micro-algaes (China box-like algae, the double tail algae of Bu Shi, wings
Root pipe algae) culture fluid component prescription is identical.
The cultivation of three kinds of microalgae of step 2
Taking the Distilled water bucket of 3 10000 milliliters washed, each bottle adds culture fluid 5000-6000 milli described in step 1
Rise, shake up, take that growth is vigorous, be in exponential phase of growth, pollution-free, purebred Chinese box-like algae, the double tail algae of Bu Shi, wing root
Algae inoculates pipe the most respectively, carries out monoculture.Inoculum density is China's ten thousand cells/ml of box-like algae 2-3, and Bu Shi is double
Ten thousand cells/ml of tail algae 4-6, wing root pipe algae ten thousand cells/ml of rotary strainer algae 3.5-5, shake up, this Distilled water bucket is put into
Microdisk electrode room is cultivated.Clog with a rubber stopper at bung, stopper is stamped 2 holes, a diameter of 5 millimeters of hole, insert
Enter varying length 2 glass tubings, glass tube external diameter 5 millimeters, a length of 70-80 centimetre of longer one, in order to inflate,
Shorter one is exhaustor, and a length of 8-10 centimetre, glass is exposed at two ends.Insert bottom long glass tubing close to drum head,
Upper end relatively soft plastics or latex tubing are connected with air compressor machine, are filled with mixed gas, and each barrel of aeration quantity is 3-5 liter/min,
Mixed gas composition is VAir∶VOzone∶VPure carbon dioxide∶VPure ammonia=98%: 1%: 0.8%: 0.2% (volume ratio), the most uninterruptedly
Inflation.Microdisk electrode room conditioning temperature control, CFL lighting, co-culture 9 days, within first 3 days, temperature is set to 21-22 DEG C, and illumination is strong
Degree is for 3000-4000lux, and within the 4-6 days, temperature is set to 23-25 DEG C, intensity of illumination 6000-7000lux, and the is set to for 7-9 days
27-29 DEG C and 8000-10000lux, fresh medium 800 milliliters described in the 6th day each barrel of interpolation step 1, to supplement
The consumption of nutritive salt, the 7th day each bottle of interpolation distilled water 500 milliliters, to supplement the loss of water caused because of inflation and to dilute algae
Liquid, by the 9th day, China's box-like algae density was up to 900,000-120 ten thousand cells/ml, and the double tail algae density of Bu Shi reaches 500,000-75
Ten thousand cells/ml, wing root pipe algae reaches 650,000-85 ten thousand cells/ml, stops cultivating, and prepares to make a gift of with this algae solution Aeschna melanictera of throwing something and feeding
Shellfish larva.
The cultivation of step 3 Perna uiridis (Linnaeus) larva
Take 1 cylindrical Maifanitum bucket, diameter 70-80 centimetre, high 65-75 centimetre, bucket thickness 2-3 centimetre, Aeschna melanictera is made a gift of
Shellfish D-shaped larva is placed in this bucket cultivation, uncovered cultivation (i.e. bucket is without adding a cover), and cultivation water is Magnetized Seawater, adds magnetization sea
Water, to depth of water 0.40-45 rice, indoor cultivation, air-conditioning temperature control, CFL lighting, adjusts the temperature to 23-26 DEG C, illumination
2000-3000lux, Perna uiridis (Linnaeus) D-shaped larva breeding density is 5-7/milliliter, throws something and feeds every day China box-like algae by each larva
Double tail algae 2000-3000 of 10000-15000, Bu Shi, the amount of wing root pipe algae 6000-8000 throws something and feeds 3 kinds of algae solutions, point
Morning, noon and afternoon throw something and feed for three times, every minor tick 8 hours, and later along with larval growth, body length often increases and is twice, and daily ration, feeding quantity also increases one
Times, uninterruptedly to inflate with air compressor machine simultaneously, be filled with mixed gas, mixed gas composition is VAir∶VOzone∶VPure carbon dioxide∶VPurity oxygen
=97%: 2%: 0.5%: 0.5%；Gas tube end connects a gas exhausting stone, and aeration quantity is 8-12 liter/min.When paedomorphosis is to becoming
During the state phase, there is 40% germling that eyespot occurs, adherance can be thrown in, with the polyethylene mesh sheet 6-12 sheet of 20 mesh after sterilization,
Being divided into 3 bundle hangings and be used as adherance in Maifanitum bucket, change water once by siphon tube method every day, quantity of exchanged water is 1/4-1/3, with
Larval growth, quantity of exchanged water is also gradually increased, and reaches 80% to roof larva later stage quantity of exchanged water；By the bolting silk mistake of 120 mesh when changing water
Filter, stop, it is to avoid larva escapes with current；Cultivate 28-32 days, when juvenile mollusk shell height is up to 0.25 centimetre, by the Seedling in mesh sheet
Scrub, i.e. may move into ocean and cultivate or sell.
The disinfectant that described polyethylene mesh sheet uses is Chinese herbal medicine composite disinfectant, and sterilization method is for soaking 20 minutes.
Wherein Chinese herbal medicine composite disinfectant constituent (ratio of weight and number): Radix seu Folium Alchorneae trewioidis 2-4 part, Herba Houttuyniae 6-9 part, Radix Bupleuri
5-8 part, Herba Glechomae 7-12 part, Cortex Dictamni 10-15 part, Rhizoma Atractylodis 7-12 part, Fructus Kochiae 8-15 part, Radix Saposhnikoviae 5-7 part, thin
Lotus 3-6 part, Radix Isatidis 0.5-0.9 part, Radix Angelicae Dahuricae 10-14 part, Herba Andrographis 5-8 part, Fructus Hippophae 2-6 part, Phellinus igniarius (L. ex Fr.) Quel. 1-3 part.
Beneficial outcomes
In order to overcome the deficiency of current technology, the invention provides the side of a kind of Maifanitum bucket High Density Cultivation Perna uiridis (Linnaeus) larva
Method, technological core is the bait first cultivating Perna uiridis (Linnaeus) larva in Distilled water bucket with Magnetized Seawater--China box-like algae, Bu Shi are double
Tail algae, wing root pipe algae, after to cultivate Perna uiridis (Linnaeus) larva China box-like algae of throwing something and feeding, Bu Shi with Magnetized Seawater in Maifanitum bucket double
Tail algae, wing root pipe algae.My research and test result indicate that, compared with conventional art, micro algae culturing liquid scientific formulation of the present invention
Rationally, algal grown is fast, and yield is high, is difficult to pollute, and easy to operate, equipment is simple, cultivates with Maifanitum bucket and Magnetized Seawater
Perna uiridis (Linnaeus) larva China box-like algae of throwing something and feeding, Bu Shi double tail algae, wing root pipe algae, larval growth is fast, and body constitution is healthy and strong, survival rate
Improve 120%.
Accompanying drawing explanation
Fig. 1 Distilled water bucket cultivates bait micro-algae figure；
Wherein arrow indication is charge air conditioning trend；Wherein China box-like algae cultivated by A bucket, and the double tail algae of Bu Shi cultivated by B bucket, and C bucket is trained
Support wing root pipe algae；
Detailed description of the invention
A kind of method of Maifanitum bucket High Density Cultivation Perna uiridis (Linnaeus) larva, method is as follows:
The preparation of three kinds of bait micro-algaes of step 1 (China box-like algae, the double tail algae of Bu Shi, wing root pipe algae) culture fluid
Micro algae culturing liquid composition (ratio of weight and number): Urina Hominis 1-1.2 part, NH4Cl 0.1-0.2 part, KH2PO4 0.02-0.04
Part, NaHCO30.8-1.2 part, MgSO4·7H2O 0.03-0.05 part, (NH4)2SO40.04-0.08 part, CaCl2·2H2O 0.05-0.08
Part, NaCl 0.8-1.2 part, Na2SiO30.04-0.06 part, CH3COONa, 0.03-0.07 part, FeSO40.06-0.09 part,
MnSO4·7H2O 0.003-0.006 part, CoCl2·6H2O 0.007-0.009 part, NiCl2·6H2O 0.003-0.006 part,
CH3CH2COONa 0.03-0.06 part, carbamide 0.2-0.4 part, titanium solution 0.5-0.8 part, thiamine hydrochloride 0.0001-0.002
Part, medical stone powder 0.2-0.5 part, Magnetized Seawater 1000 parts.Three kinds of bait micro-algaes (China box-like algae, the double tail algae of Bu Shi, wings
Root pipe algae) culture fluid component prescription is identical.
The cultivation of three kinds of microalgae of step 2
Taking the Distilled water bucket of 3 10000 milliliters washed, each bottle adds culture fluid 5000-6000 milli described in step 1
Rise, shake up, take that growth is vigorous, be in exponential phase of growth, pollution-free, purebred Chinese box-like algae, the double tail algae of Bu Shi, wing root
Algae inoculates pipe the most respectively, carries out monoculture.Inoculum density is China's ten thousand cells/ml of box-like algae 2-3, and Bu Shi is double
Ten thousand cells/ml of tail algae 4-6, wing root pipe algae ten thousand cells/ml of rotary strainer algae 3.5-5, shake up, this Distilled water bucket is put into
Microdisk electrode room is cultivated.Clog with a rubber stopper at bung, stopper is stamped 2 holes, a diameter of 5 millimeters of hole, insert
Enter varying length 2 glass tubings, glass tube external diameter 5 millimeters, a length of 70-80 centimetre of longer one, in order to inflate,
Shorter one is exhaustor, and a length of 8-10 centimetre, glass is exposed at two ends.Insert bottom long glass tubing close to drum head,
Upper end relatively soft plastics or latex tubing are connected with air compressor machine, are filled with mixed gas, and each barrel of aeration quantity is 3-5 liter/min,
Mixed gas composition is VAir∶VOzone∶VPure carbon dioxide∶VPure ammonia=98%: 1%: 0.8%: 0.2% (volume ratio), the most uninterruptedly
Inflation.Microdisk electrode room conditioning temperature control, CFL lighting, co-culture 9 days, within first 3 days, temperature is set to 21-22 DEG C, and illumination is strong
Degree is for 3000-4000lux, and within the 4-6 days, temperature is set to 23-25 DEG C, intensity of illumination 6000-7000lux, and the is set to for 7-9 days
27-29 DEG C and 8000-10000lux, fresh medium 800 milliliters described in the 6th day each barrel of interpolation step 1, to supplement
The consumption of nutritive salt, the 7th day each bottle of interpolation distilled water 500 milliliters, to supplement the loss of water caused because of inflation and to dilute algae
Liquid, by the 9th day, China's box-like algae density was up to 900,000-120 ten thousand cells/ml, and the double tail algae density of Bu Shi reaches 500,000-75
Ten thousand cells/ml, wing root pipe algae reaches 650,000-85 ten thousand cells/ml, stops cultivating, and prepares to make a gift of with this algae solution Aeschna melanictera of throwing something and feeding
Shellfish larva.
The cultivation of step 3 Perna uiridis (Linnaeus) larva
Take 1 cylindrical Maifanitum bucket, diameter 70-80 centimetre, high 65-75 centimetre, bucket thickness 2-3 centimetre, Aeschna melanictera is made a gift of
Shellfish D-shaped larva is placed in this bucket cultivation, uncovered cultivation (i.e. bucket is without adding a cover), and cultivation water is Magnetized Seawater, adds magnetization sea
Water, to depth of water 0.40-45 rice, indoor cultivation, air-conditioning temperature control, CFL lighting, adjusts the temperature to 23-26 DEG C, illumination
2000-3000lux, Perna uiridis (Linnaeus) D-shaped larva breeding density is 5-7/milliliter, throws something and feeds every day China box-like algae by each larva
Double tail algae 2000-3000 of 10000-15000, Bu Shi, the amount of wing root pipe algae 6000-8000 throws something and feeds 3 kinds of algae solutions, point
Morning, noon and afternoon throw something and feed for three times, every minor tick 8 hours, and later along with larval growth, body length often increases and is twice, and daily ration, feeding quantity also increases one
Times, uninterruptedly to inflate with air compressor machine simultaneously, be filled with mixed gas, mixed gas composition is VAir∶VOzone∶VPure carbon dioxide∶VPurity oxygen
=97%: 2%: 0.5%: 0.5%；Gas tube end connects a gas exhausting stone, and aeration quantity is 8-12 liter/min.When paedomorphosis is to becoming
During the state phase, there is 40% germling that eyespot occurs, adherance can be thrown in, with the polyethylene mesh sheet 6-12 sheet of 20 mesh after sterilization,
Being divided into 3 bundle hangings and be used as adherance in Maifanitum bucket, change water once by siphon tube method every day, quantity of exchanged water is 1/4-1/3, with
Larval growth, quantity of exchanged water is also gradually increased, and reaches 80% to roof larva later stage quantity of exchanged water；By the bolting silk mistake of 120 mesh when changing water
Filter, stop, it is to avoid larva escapes with current；Cultivate 28-32 days, when juvenile mollusk shell height is up to 0.25 centimetre, by the Seedling in mesh sheet
Scrub, i.e. may move into ocean and cultivate or sell.
The disinfectant that described polyethylene mesh sheet uses is Chinese herbal medicine composite disinfectant 0.4-0.7 part.
Wherein Chinese herbal medicine composite disinfectant constituent (ratio of weight and number): Radix seu Folium Alchorneae trewioidis 2-4 part, Herba Houttuyniae 6-9 part, Radix Bupleuri
5-8 part, Herba Glechomae 7-12 part, Cortex Dictamni 10-15 part, Rhizoma Atractylodis 7-12 part, Fructus Kochiae 8-15 part, Radix Saposhnikoviae 5-7 part, thin
Lotus 3-6 part, Radix Isatidis 0.5-0.9 part, Radix Angelicae Dahuricae 10-14 part, Herba Andrographis 5-8 part, Fructus Hippophae 2-6 part, Phellinus igniarius (L. ex Fr.) Quel. 1-3 part.
NH described in step 14Cl, KH2PO4, NaHCO3, MgSO4·7H2O, (NH4)2SO4, CaCl2·2H2O, NaCl, Na2SiO3,
CH3COONa, FeSO4, MnSO4·7H2O, CoCl2·6H2O, NiCl2·6H2O, CH3CH2COONa chemical drugs being both needed to of purity
Learn pure rank；But carbamide can be common agrochemical rank；
Urina Hominis preparation method described in step 1: take urine, natural fermentation 72 hours；Take one 3000 milliliters of conical flasks, add
Enter the urine 1500 milliliters fermented, heating, boil 4-5 minute, cooling, standby；
Titanium solution preparation method described in step 1 (weight ratio): 0.7366 part of titanium oxalate, is dissolved in 100 parts of distilled water,
Add 5 parts of ammonium hydroxide, be allowed to into alkalescence, precipitate, by centrifuging, precipitation is concentrated, with the sulfur of 20 parts of 0.05mol/L
Acid is dissolved and get final product；
Described in step 2, three kinds of microalgae are China box-like algaes, the double tail algae of Bu Shi, wing root pipe algae；
In step 2, inoculation is with China box-like algae, the double tail algae of Bu Shi, wing root pipe algae carry out in advance purifying (this area conventional method,
As long as realizing purifying, such as Michaelis extraction method), during to guarantee inoculation, algae kind is purebred and is in the life of eugonic index
For a long time；
Magnetized Seawater preparation method described in step 1 and 3: take sea water, absorbent cotton filters, heating, boils 3-5 minute, cooling,
After flowing through water magnetizing appts, it is magnetic water；Water magnetizing appts magnetic induction is 0.3T, and flow is 2-3t/h, and flow velocity is 0.1-0.2
m/s；
In step 1, various nutritive salt to be added by the order provided in formula, to avoid chemical reaction；
In step 2, rubber stopper used, glass tubing are both needed to wash after being placed in 65-75 DEG C of baking oven drying and use；
Medical stone powder preparation method described in step 1: take Maifanitum, pulverizer is crushed to floury, and granule can pass through 100-120
Mesh sieve thin,tough silk；
Seeded process described in step 2 need to be carried out on aseptic operating platform；
With polyethylene mesh sheet sterilization method described in step 3: mesh sheet is washed, after be placed on (weight in 5% malachite green solution
Than) soak 12 hours, after rinse well with boiling refrigerated sea water；
Adherance described in step 3, without polyethylene mesh sheet, available coir rope replaces, and usage is identical with consumption, but polyethylene
After mesh sheet attachment, cultivating survival rate is high and growth is fast, and output of seedling is higher, it is therefore proposed that the most handy polythene net sheet spatfall；
During larva described in step 3 is cultivated, water temperature must keep stable, and temperature difference per day is less than 1 DEG C, otherwise easily causes germling and sinks to the bottom
Dead.
Last it is noted that above example is only in order to illustrate technical scheme, it is not intended to limit；Although reference
The present invention has been described in detail by previous embodiment, it will be understood by those within the art that: it still can be to front
State the technical scheme described in embodiment to modify, or wherein portion of techniques feature is carried out equivalent；And these amendments
Or replace, do not make the essence of appropriate technical solution depart from the spirit and scope of embodiment of the present invention technical scheme.
Claims (1)
1. the method with Maifanitum bucket High Density Cultivation Perna uiridis (Linnaeus) larva, it is characterised in that step is as follows:
The preparation of three kinds of bait micro-algae culture fluid of step 1；
Described three kinds of bait micro-algaes are China box-like algae tail algae double with Bu Shi and wing root pipe algae；
Micro algae culturing liquid composition, as follows according to ratio of weight and number: Urina Hominis 1-1.2 part, NH4Cl0.1-0.2 part, KH2PO40.02-0.04
Part, NaHCO30.8-1.2 part, MgSO4·7H2O0.03-0.05 part, (NH4)2SO40.04-0.08 part, CaCl2·2H2O0.05-0.08
Part, NaCl0.8-1.2 part, Na2SiO30.04-0.06 part, CH3COONa, 0.03-0.07 part, FeSO40.06-0.09 part,
MnSO4·7H2O0.003-0.006 part, CoCl2·6H2O0.007-0.009 part, NiCl2·6H2O0.003-0.006 part,
CH3CH2COONa0.03-0.06 part, carbamide 0.2-0.4 part, titanium solution 0.5-0.8 part, thiamine hydrochloride 0.0001-0.002
Part, medical stone powder 0.2-0.5 part, Magnetized Seawater 1000 parts；Three kinds of bait micro-algaes (China box-like algae, the double tail algae of Bu Shi, wings
Root pipe algae) culture fluid component prescription is identical；
The cultivation of three kinds of microalgae of step 2；
Taking the Distilled water bucket of 3 10000 milliliters washed, each bottle adds culture fluid 5000-6000 milli described in step 1
Rise, shake up, take that growth is vigorous, be in exponential phase of growth, pollution-free, purebred Chinese box-like algae, the double tail algae of Bu Shi, wing root
Algae inoculates pipe the most respectively, carries out monoculture；Inoculum density is China's ten thousand cells/ml of box-like algae 2-3, and Bu Shi is double
Ten thousand cells/ml of tail algae 4-6, wing root pipe algae ten thousand cells/ml of rotary strainer algae 3.5-5, shake up, this Distilled water bucket is put into
Microdisk electrode room is cultivated；Clog with a rubber stopper at bung, stopper is stamped 2 holes, a diameter of 5 millimeters of hole, insert
Enter varying length 2 glass tubings, glass tube external diameter 5 millimeters, a length of 70-80 centimetre of longer one, in order to inflate,
Shorter one is exhaustor, a length of 8-10 centimetre；Inserting close to drum head bottom long glass tubing, upper end is with relatively soft plastics
Or latex tubing is connected with air compressor machine, being filled with mixed gas, each barrel of aeration quantity is 3-5 liter/min, and mixed gas composition is VEmpty Gas∶VOzone∶VPure carbon dioxide∶VPure ammonia=98%: 1%: 0.8%: 0.2% (volume ratio), the most uninterrupted inflation；Microdisk electrode is indoor
Air-conditioning temperature control, CFL lighting, co-culture 9 days, within first 3 days, temperature is set to 21-22 DEG C, and intensity of illumination is 3000-4000lux,
Within the 4-6 days, temperature is set to 23-25 DEG C, intensity of illumination 6000-7000lux, and is set to 27-29 DEG C and 8000-10000 for 7-9 days
Lux, fresh medium 800 milliliters described in the 6th day each barrel of interpolation step 1, with the consumption of the salt that supplements the nutrients, the 7th day every
Individual bottle adds distilled water 500 milliliters, to supplement the loss of water caused because of inflation and to dilute algae solution, by the 9th day, and China's box-like
Algae density is up to 900,000-120 ten thousand cells/ml, and the double tail algae density of Bu Shi reaches 500,000-75 ten thousand cells/ml, wing root pipe
Algae reaches 650,000-85 ten thousand cells/ml, stops cultivating, and prepares to throw something and feed Perna uiridis (Linnaeus) larva by this algae solution；
The cultivation of step 3 Perna uiridis (Linnaeus) larva；
Take 1 cylindrical Maifanitum bucket, diameter 70-80 centimetre, high 65-75 centimetre, bucket thickness 2-3 centimetre, Aeschna melanictera is made a gift of
Shellfish D-shaped larva is placed in this bucket cultivation, and uncovered cultivation (i.e. bucket is without adding a cover), cultivation water is Magnetized Seawater, adds Magnetized Seawater
To depth of water 0.40-45 rice, indoor cultivation, air-conditioning temperature control, CFL lighting, adjust the temperature to 23-26 DEG C, illumination
2000-3000lux, Perna uiridis (Linnaeus) D-shaped larva breeding density is 5-7/milliliter, throws something and feeds every day China box-like algae by each larva
Double tail algae 2000-3000 of 10000-15000, Bu Shi, the amount of wing root pipe algae 6000-8000 throws something and feeds 3 kinds of algae solutions, point
Morning, noon and afternoon throw something and feed for three times, every minor tick 8 hours, and later along with the growth of larva, body length often increases and is twice, and daily ration, feeding quantity also increases
One times, uninterruptedly inflating with air compressor machine simultaneously, be filled with mixed gas, mixed gas composition is VAir∶VOzone∶VPure carbon dioxide∶VPure Oxygen=97%: 2%: 0.5%: 0.5%；Gas tube end connects a gas exhausting stone, and aeration quantity is 8-12 liter/min；When paedomorphosis extremely
During period of metamorphosis, there is 40% germling that eyespot occurs, adherance can be thrown in, with the polyethylene mesh sheet 6-12 sheet of 20 mesh after sterilization,
Being divided into 3 bundle hangings and be used as adherance in Maifanitum bucket, change water once by siphon tube method every day, quantity of exchanged water is 1/4-1/3, with
Larval growth, quantity of exchanged water is also gradually increased, and reaches 80% to roof larva later stage quantity of exchanged water；By the bolting silk mistake of 100 mesh when changing water
Filter, stop, it is to avoid larva escapes with current；Cultivate 28-32 days, when juvenile mollusk shell height is up to 0.25 centimetre, by the Seedling in mesh sheet
Scrub, ocean can be moved into and cultivate or sell, to sum up be present invention Maifanitum bucket High Density Cultivation Perna uiridis (Linnaeus) larva
Method.
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| CN113243341A (en) * | 2021-06-22 | 2021-08-13 | 中国人民解放军国防科技大学 | Barnacle larva breeding device, barnacle larva breeding method, antifouling test system, breeding-antifouling test system and antifouling test method |
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