CN105699147A - Multichannel slide glass mounting slot device - Google Patents
Multichannel slide glass mounting slot device Download PDFInfo
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- CN105699147A CN105699147A CN201610160720.3A CN201610160720A CN105699147A CN 105699147 A CN105699147 A CN 105699147A CN 201610160720 A CN201610160720 A CN 201610160720A CN 105699147 A CN105699147 A CN 105699147A
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- slide glass
- slot
- container
- multichannel
- plate
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
Abstract
The invention discloses a heatable multichannel slide glass mounting slot device. The multifunctional slide glass mounting slot device mainly comprises a slot mounting container, cover plates, slide glass, plate springs, a left-side bracket, a right-side bracket and a heating device, wherein the slot mounting container is made of a high-temperature-resistant material. Slots are arranged vertically or obliquely, and can hold a plurality of slide glass specimens to be detected compactly; the slide glass and the cover plates can be detached easily and conveniently from the slots; the specimens can be subjected to heating and heat preservation in the container in order that the specimens do not need to be transferred into another container which can be heated in a specimen treating process, the slide glass specimens are always treated in the same device, and manual operation steps are simplified or an automatic flow is integrated in conjunction with an instrument having a specimen adding function. By adopting the multichannel slide glass mounting slot device, sliced specimens are always covered with a reagent in the specimen treating process, the reliability and repeatability of a thermal treatment process are improved, the operation steps are simplified, the specimen treating quality is improved, and full automatic operation can be integrated conveniently.
Description
Technical field
The invention belongs to biological sample process field, particularly relate to a kind of multichannel slide glass and slot apparatus is installed。
Background technology
The whole process of immunohistochemistry needs nearly 30 steps, hatch including: a) dewaxing, aquation, cleaning, high-temperature hot reparation, cooling, cleaning, primary antibodie, clean, drain with wiping, two resist and hatch, clean, drain and hatch with wiping, synergist, clean, drain and the step such as wiping, colour developing, cleaning, lining dye, cleaning, dehydration, mounting。Impact by the technical merit between hospital and hospital and operating habit difference, it is difficult to standardization。The efficiency and the reliability that improve sample disposal become the task of top priority。
At present in checkout procedure, specimen section tiling is laid, and takes up space big；Reagent pipetting volume sprays application of sample above thin slice, and load position error is big, and reagent covering in section is with randomness, it is difficult to guaranteeing to cover whole section, some specimen do not obtain the covering of reagent and false negative occur。Needing high-temperature heating in sample disposal process, this is to make antigen or pathogen characteristic protein reply step necessary to original shape。It is maintain the whole vital factor of detection specificity that antigen or pathogen characteristic protein restore to the original state。Heating requirements 80-100 DEG C or higher temperature also maintain 5-40 minute。Most common method is that pressure cooker boils, generally boils and boil or the method such as hot water temperature bath。
But, the container of at present conventional slide glass sample disposal or carrier do not possess can heating function or possess the resistance to performance being heated。Need in the midway of slide glass sample disposal to transfer in heatable container slide glass specimen to carry out, then need again to transfer on the operating board of room temperature, add complexity and the operating error of operating process。
Realize slide glass specimen to be heated in position processing and can greatly simplify slide glass specimen processing operation step, improve the repeatability of slide glass sample disposal result and be easier to carry out full-automatic operation flow process, meet the simpler reliable requirement of slide glass sample disposal。
Summary of the invention
In order to solve the problems referred to above, the present invention proposes a kind of multichannel slide glass and installs slot apparatus, and between cover plate and slide glass, precision-fit forms the molectron of a relative closure, and the container of sample disposal arranges the installation slot of inclination, the compact placement of molectron can be made, take minimum space；Forming fine gap when slide glass cover plate and slide glass combination, this micro gap can produce capillary siphonage so that application of sample reagent passes through capillary siphonage uniform fold specimen on slide glass；The slot device of assigning of the present invention uses exotic material to make and possess Contiuum type or separate type heater。Meet that to save space, section preparation application of sample uniformly reliable simultaneously, can the requirement of heat treated etc. in position。
The purpose of the present invention realizes at least through one of following technical scheme。
Slot apparatus installed by a kind of multichannel slide glass, including by slot container, cover plate, slide glass, flat spring, left side brackets and right side support；Being mirror image between left side brackets and right side support, both collectively form the installation slot rest of slide glass and cover plate combination part, install slot rest and slide glass cover plate assembly is arranged in by slot container；Left side brackets includes the U-type groove in side plate and the more than one projecting plate face that is fixed on side plate, and more than one U-type groove is arranged along side plate length linear array, and U-type groove is made up of bracer of drawing money on credit, short bracer and bottom limited block；The short support block length of length ratio of described bracer of drawing money on credit, and both sides are parallel；U-type groove is obliquely installed and short bracer is positioned on bracer of drawing money on credit；Flat spring includes the waveform reed and the location-plate that link into an integrated entity；Waveform reed a side of location-plate and and location-plate between there is an opening so that flat spring can be enclosed within short bracer of specular on left side brackets and right side support；Location-plate is fixed on short bracer, produces support force, and waveform reed has the wavy bulge that can produce elastic deformation；The molectron that slide glass and cover plate coordinate, the U-type groove along support is inserted, the waveform reed of the surface compacting flat spring of cover plate, and the waveform reed generation spring pressure that is squeezed is applied on slide glass and cover plate, uses compression between slide glass and cover plate。
Further optimally, install bottom slot rest and be provided with runners so that slide glass and cover plate combination part with keep at a distance by slot container bottom。
Further optimally, the angle that U-type groove tilts is 1 ~ 90 degree。
Further optimally, the angle that U-type groove tilts is 15 ~ 90 degree。
Further optimally, the waveform reed of flat spring and location-plate adopt sheet copper, spring steel or other elastomeric material to make；Location-plate is fixed on short bracer by bonding way。
Further optimally, the upper ends of slide glass for adhesive label and bar code or identifies labelling；Leave spacing between the plurality of U-type groove, make the bar code of slide glass paste position and do not blocked by adjacent slide glass。
Further optimally, each by string or multiple row peace slot rest can be arranged in slot container, make slide glass specimen in by slot container with single row or multiple rows and single-row or multiple rows of close-packed arrays。
Further optimally, by the installation slot rest arranging more than string in slot container, and in adjacent two the installation slot rest being wherein parallel to each other, also there is between adjacent left side brackets and right side support more than one described U-type groove structure, between namely adjacent left side brackets and right side support, also form described installation slot rest。
Further optimally, every string is installed the inserted sheet quantity of U-type groove in slot rest and can be configured 1-100 as required and open, and is generally 2 ~ 50 (namely 2-50 arranges)。By slot container, installation slot rest described in multiple row laid parallel is set, or each by installation slot rest described in multiple row laid parallel can be arranged in slot container, define the arrangement of the slide glass specimen of the cloth configuration of multiple rows of multiple row。1-100 row and 1 ~ 100 row can be set as required, 2-50 row and 1-40 row normally can be set。
Further optimally, multichannel slide glass is installed slot apparatus and is adopted tolerance 80oThe material of C temperatures above is made。
Further optimally, water intaking valve, outlet valve are set by slot container bottom, for manually or automatically controlling feeding and the discharge opeing of container；
Further optimally, the described feeding by slot container and discharge opeing are controlled by external plug-in type feeding and drainage system。
Further optimally, described cover plate includes capillary plane, depth localization face, width locating piece, bottom locating piece, groove side and channel opening face；The both sides of described capillary plane are provided above two depth localization faces, and capillary plane is parallel with depth localization face；Described depth localization face has two, lays respectively at the left and right sides of capillary plane；The plane of the described slide glass part near both sides of the edge is fitted with depth localization face, makes one capillary gap of formation between slide surface and capillary plane；Each depth localization face is provided with more than one described width locating piece away from the outer ledge of capillary plane；Distance between two locating pieces in left and right mates to play position-limiting action with the width of slide glass；Bottom locating piece is arranged on the bottom in depth localization face；Described channel opening face is connected with capillary plane and forms 1-175 degree angle A1, is generally 30-105 degree；Both sides, channel opening face are respectively connected to a groove side；Described channel opening face and two groove sides and slide glass plane collectively form the loading slot of and described capillary gap area。
Relative to prior art, the present invention has the following advantages and technique effect:
The slot of the present invention often group can arrange by slotting multiple slide glass specimen, and what form that string can assign multiple rows of slide glass specimen assigns groove；Between the column and the column can individually or disjunctor arrange, form the setting of structuring the formation of single row or multiple rows and single-row or multiple row, can closely assign the slide glass specimen to be checked of volume；Cooperation between slide glass and cover plate is compact accurately, produces a capillary gap to guarantee that slide glass specimen obtains the covering of reagent all the time；Slide glass and cover plate load and unload simple and convenient from slot；By slot apparatus possess heat-resisting and can heating function, specimen can fully, uniformly heat and be incubated in by slot container, fundamentally ensures the reproducibility and reliability of hot repair process。Container can tolerate higher temperature, thus will need not separately process, at sheet cover plate and slide glass, the step that then there is a need to reconfigure when sample disposal process needs high-temperature to process, can be implemented in sheet specimen from start to finish to carry out in same device, simplify manual operations, with have the instrument of application of sample function in conjunction with time, just can be integrated into full-automatic operation flow process。
Accompanying drawing explanation
Fig. 1 is the structural representation that slot apparatus installed by a kind of multichannel slide glass in example。
Fig. 2 is the structural representation that slot apparatus (removing container) installed by a kind of multichannel slide glass in example。
Fig. 3 is the structural representation that slot apparatus (removing container and right side support) installed by a kind of multichannel slide glass in example。
Fig. 4 is the front projection view of Fig. 3。
Fig. 5 is the close-up schematic view of Fig. 3。
Fig. 6 is the structural representation of support。
Fig. 7 is the front projection view of Fig. 6。
Fig. 8 is the structural representation of flat spring。
Fig. 9 is slide glass and the structural representation of slide glass cover plate combination part。
Figure 10 is the exemplary construction schematic diagram of a kind of improvement of cover plate。
Figure 11 is the structural representation that the present invention is combined with heated base。
Figure 12 is the generalized section of Figure 11。
Figure 13 is the structural representation that the present invention arranges multiple rows of multiple row slide glass slot。
Detailed description of the invention
Although present invention encompasses various amendment and alternative method and device, but embodiments of the present invention showing in the accompanying drawings and will be described in more detail below。It is, however, to be understood that concrete description and accompanying drawing are not intended to limit the invention to disclosed concrete form。
As shown in Fig. 1 ~ Fig. 5, slot apparatus installed by a kind of multichannel slide glass, mainly includes by parts such as slot container 1, cover plate 2, slide glass 3, flat spring 4, left side brackets 5, right side supports 6,
Left side brackets 5 and right side support 6 belong to the character shape of specular, and both collectively form the installation slot rest of slide glass cover plate assembly, and both spacing can regulate as required；Such as Fig. 6, Fig. 7, left side brackets 5 is provided with the U-type groove in more than one projecting plate face, and this U-type groove includes draw money on credit bracer 51, short bracer 52 and bottom limited block 53；Short bracer 52 of the length ratio of described bracer 51 of drawing money on credit is long, and both sides are parallel；Described U-type groove is fixed on side plate 54, and erects or tilt to put, and angle is 1 ~ 90 degree, is generally 15-90 degree；Described U-type groove is arranged with single and multiple rows of and single-row and multiple row array, and the row of layout and columns can be arranged according to user's request。Fig. 5 is described in dotted line one group of slide glass and cover plate assembly is arranged in a U-type groove。
Such as Fig. 8, flat spring 4 includes waveform reed 41 and location-plate 42, adopts sheet copper, spring steel or other elastomeric material to make；There is between waveform reed 41 and location-plate 42 opening so that flat spring 4 can put on short bracer 52；Location-plate 42 is fixed on short bracer 52 by the mode such as bonding, produces support force；The wavy bulge of waveform reed 41, can form certain elastic deformation。
The molectron that slide glass 3 and cover plate 2 coordinate, the U-type groove along support is inserted, the waveform reed of the surface compacting flat spring 4 of cover plate 2, produces spring pressure and is applied on slide glass 3 and cover plate 2 with both compressions, in order to avoid in the dead of night。
By left side brackets 5 and right side support 6 and slide glass cover plate assembly thereon, it is arranged on by slot container 1, frame bottom is provided with runners 55, make slide glass 3 and cover plate 2 molectron and by keeping certain distance bottom slot container 1, it is simple to add hydrothermal solution and pass to and avoid bottom hot-spot phenomenon at bottom stream。
Implementing ground further, the upper ends of slide glass 3 is for adhesive label and bar code or identifies labelling etc.；Appropriately distance should be kept between the multiple U-type groove arranged in left side brackets 5 and right side support 6。
Further, left side brackets 5 and right side support 6 form string and install slot rest, and every string is installed the quantity of U-type groove in slot rest and can be configured as required being 1 ~ 100, and 1-100 can be assigned to open slide glass specimen, is generally 2-50。
Such as Figure 13, can be arranged as required to, by slot container 1, the installation slot rest that multiple row is parallel, it is possible to 1 ~ 100 row are set, be generally 1-40 row；Each group (row) are installed slot rest and are included left side brackets 5 and right side support 6；Adjacent two rows install adjacent left side brackets 5 and right side support 6 in slot rest and also can permeate a part, as long as having both left side brackets 5 and the function of right side support 6。
Further, can arranging water intaking valve and drain valve () at described container bottom, water valve can be manually or electrically valve, for manually or automatically controlling feeding and the discharge opeing of container in figure slightly；The feeding of described container and discharge opeing, it is also possible to undertaken by external plug-in type feeding and drainage system。
Such as Fig. 9, Figure 10, implement ground further, cover plate can also just being optimized and improve, for instance as a preferred example, described cover plate includes capillary plane 101, depth localization face 102, width locating piece 103, bottom locating piece 104, groove side 106 and channel opening face 107；
The both sides of described capillary plane 101 are provided above two depth localization faces 102, and capillary plane 101 is parallel with depth localization face；Described depth localization face 102 has two, lays respectively at the left and right sides of capillary plane 101；The plane of the described slide glass part near both sides of the edge is fitted with depth localization face 102, makes one capillary gap of formation between slide surface and capillary plane 101；Each depth localization face 102 is provided with more than one described width locating piece 103 away from the outer ledge of capillary plane 101；Distance between two locating pieces in left and right mates to play position-limiting action with the width of slide glass；Bottom locating piece 104 is arranged on the bottom in depth localization face 102；The bottom of capillary plane 101 is provided with bottom boss 105.
Described channel opening face 107 is connected with capillary plane 101 and forms 1 ~ 175 degree of Plane Angle angle A1；Both sides, channel opening face 107 are respectively connected to a groove side 106；Described channel opening face 107 and two groove sides 106 and slide glass plane collectively form the loading slot 109 of and described capillary gap area。
Such as Figure 11, Figure 12, this example also includes be positioned at the heater by slot container 1 bottom part down, heater be Contiuum type or separate type by slot container 1, such as adopt disjunctor or separate type heating rod, heating plate, Electromagnetic Heating induction system carries out electromagnetic induction heating or perfusion hot liquid carries out temperature bath and heats。Hot plate 122 is provided with and adds heat pipe () in figure slightly, be arranged in heater canister 123；Add heat pipe to be connected with external power supply, and controlled power and the time of heating in figure slightly by controller ()；Add heat produced by heat pipe and pass to hot plate 122。It is made of metal by slot container 1, is operationally placed on hot plate 122, accept the heat of hot plate 122 to heat by the liquid in slot container 1。The top of slot container 1 being provided with upper water inlet 124 and bottom is provided with lower discharge outlet 112, upper water inlet 124 is coaxial with lower discharge outlet 112；Upper water inlet and lower discharge outlet 124 are provided with valve () in figure slightly, can be controlled the opening and closing at the upper and lower mouth of a river 124 by manually or electrically controller () in figure slightly。Heater canister 123 is provided with a spike 111；Heater canister 123 is made up of insulation material, plays the effect of insulation。
Case study on implementation: slide glass cover plate is sized such that depth localization face 2 length 54mm, distance between inside two width locating pieces 3 is 20mm, D1=0.15mm, D2=0.5mm, the length of capillary plane is 40mm, width is 20mm, and the angle between channel opening face and depth localization face is 30 degree。The length 75mm of slide glass, width 25mm。U-shaped slot tilts 45 degree, and draw money on credit spacing 7mm, container depth 41mm between bracer and short bracer。Multichannel slide glass is installed slot apparatus and is adopted tolerance 80oThe material of C temperatures above makes (such as Merlon)。Experiments show that slot compact can insert multiple slide glass slide glass specimen to be checked；Cooperation between slide glass and cover plate is compact accurately；Slide glass and cover plate load and unload simple and convenient from slot；Specimen can fully, uniformly heat and be incubated in a reservoir, fundamentally ensure hot repair process reproducibility and reliability, ensure reagent to specimen being completely covered from start to finish, ensure uniformity and repeatability that reagent covers。
The slot of this example can be erect or be obliquely installed, can compact placement multiple slide glass specimen to be checked；Cooperation between slide glass and cover plate is compact accurately；Slide glass and cover plate load and unload simple and convenient from slot；Specimen can be heated in container and be incubated, make specimen need not be transferred in other heatable container by sample disposal process to carry out, be beneficial to simplify manual steps or reorganization automatic flow, and fundamentally ensure hot repair process required in sample disposal process reproducibility and reliability, ensure reagent to specimen being completely covered from start to finish, ensure uniformity and repeatability that reagent covers。Container can tolerate higher temperature and possess Contiuum type or separate type heating function, thus will need not separately process, at sheet cover plate and slide glass, the step that then there is a need to reconfigure when sample disposal process needs high-temperature to process, can be implemented in sheet specimen from start to finish to carry out in same device, it is simple to carry out full-automatic operation。
The announcement of book and instruction according to the above description, above-mentioned embodiment can also be modified and revise by those skilled in the art in the invention。Therefore, the invention is not limited in detailed description of the invention disclosed and described above, should also be as some modifications and changes of the present invention falling in the scope of the claims of the present invention。
Claims (10)
1. slot apparatus installed by a multichannel slide glass, it is characterised in that include by slot container (1), cover plate (2), slide glass (3), flat spring (4), left side brackets (5) and right side support (6)；
Being mirror image between left side brackets (5) and right side support (6), both collectively form slide glass (3) and the peace slot rest of cover plate (2) molectron, install slot rest and slide glass cover plate assembly is arranged in by slot container (1)；Left side brackets (5) includes the U-type groove in side plate (54) and the more than one projecting plate face that is fixed on side plate (54), more than one U-type groove is arranged along side plate (54) length linear array, and U-type groove is made up of bracer of drawing money on credit (51), short bracer (52) and bottom limited block (53)；Short bracer (52) of the length ratio of described bracer of drawing money on credit (51) is long, and both sides are parallel；U-type groove is obliquely installed and short bracer (52) is positioned on bracer of drawing money on credit (51), and the angle that U-type groove tilts is 1 ~ 90 degree；
Flat spring (4) includes the waveform reed (41) and the location-plate (42) that link into an integrated entity；Waveform reed (41) be positioned at location-plate (42) a side and and location-plate (42) between there is an opening so that flat spring (4) can be enclosed within short bracer (52) of the upper specular of left side brackets (5) and right side support (6)；Location-plate (42) is fixed on short bracer (52), produces support force, and waveform reed (41) has the wavy bulge that can produce elastic deformation；
The molectron that slide glass (3) and cover plate (2) coordinate, U-type groove along support is inserted, the waveform reed on surface compacting flat spring (4) of cover plate (2), waveform reed be squeezed generation spring pressure be applied on slide glass (3) and cover plate (2), use slide glass (3) and cover plate (2) between compression。
2. slot apparatus installed by a kind of multichannel slide glass according to claim 1, it is characterised in that: install and be provided with runners (55) bottom slot rest so that slide glass (3) and cover plate (2) molectron are kept at a distance with by slot container (1) bottom。
3. slot apparatus installed by a kind of multichannel slide glass according to claim 1, it is characterised in that: also include being positioned at the heater by slot container (1) bottom part down, heater be Contiuum type or separate type by slot container (1)。
4. slot apparatus installed by a kind of multichannel slide glass according to claim 1, it is characterised in that: the waveform reed (41) of flat spring (4) and location-plate (42) adopt sheet copper, spring steel or other elastomeric material to make；Location-plate (42) is fixed on short bracer (52) by bonding way。
5. slot apparatus installed by a kind of multichannel slide glass according to claim 1, it is characterised in that: left side brackets (5) and right side support (6) composition string install slot rest, and each column can assign the slide glass specimen of more than。
6. slot apparatus installed by a kind of multichannel slide glass according to claim 5, it is characterized in that: by the installation slot rest arranging more than string in slot container (1), and in adjacent two the installation slot rest being wherein parallel to each other, also there is between adjacent left side brackets (5) and right side support (6) more than one described U-type groove structure, between namely adjacent left side brackets (5) and right side support (6), also form described installation slot rest。
7. slot apparatus installed by a kind of multichannel slide glass according to claim 1, it is characterised in that: multichannel slide glass is installed slot apparatus and is adopted tolerance 80oThe material of C temperatures above is made。
8. slot apparatus installed by a kind of multichannel slide glass according to claim 1, it is characterised in that: multichannel slide glass installs slot apparatus employing disjunctor or separate type heating rod, heating plate, EM induction system carries out electromagnetic induction heating or perfusion hot liquid carries out temperature bath heating。
9. slot apparatus installed by a kind of multichannel slide glass according to claim 1, it is characterised in that: water intaking valve and outlet valve are set by slot container (1) bottom, for the feeding manually or automatically controlled to slot container and discharge opeing；Or the described feeding by slot container (1) and discharge opeing can also by external plug-in type feeding and drainage systems。
10. slot apparatus installed by a kind of multichannel slide glass according to claim 1, it is characterised in that: the both sides of described capillary plane are provided above two depth localization faces, and capillary plane is parallel with depth localization face；Described depth localization face has two, lays respectively at the left and right sides of capillary plane；The plane of the described slide glass part near both sides of the edge is fitted with depth localization face, makes one capillary gap of formation between slide surface and capillary plane；Each depth localization face is provided with more than one described width locating piece away from the outer ledge of capillary plane；Distance between two locating pieces in left and right mates to play position-limiting action with the width of slide glass；Bottom locating piece is arranged on the bottom in depth localization face；Described channel opening face is connected with capillary plane and forms 1 ~ 175 degree of plane angle (A1)；Both sides, channel opening face are respectively connected to a groove side；Described channel opening face and two groove sides and slide glass plane collectively form the loading slot of and described capillary gap area。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610160720.3A CN105699147B (en) | 2016-03-21 | 2016-03-21 | A kind of multichannel slide glass installation slot apparatus |
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| CN201610160720.3A CN105699147B (en) | 2016-03-21 | 2016-03-21 | A kind of multichannel slide glass installation slot apparatus |
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| CN105699147B CN105699147B (en) | 2019-04-12 |
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| CN106248470A (en) * | 2016-08-31 | 2016-12-21 | 广州深达生物制品技术有限公司 | A kind of slide mount device of slide glass sample disposal |
| CN108132172A (en) * | 2016-12-01 | 2018-06-08 | 辽东学院 | Multifunctional combination dye vat |
| CN109061233A (en) * | 2018-07-27 | 2018-12-21 | 广州华望汽车电子有限公司 | A kind of multipurpose drawer and its trolley |
| CN113039472A (en) * | 2018-11-02 | 2021-06-25 | 豪洛捷公司 | Digital imaging system and method |
| CN114354317A (en) * | 2022-01-14 | 2022-04-15 | 厦门通灵生物医药科技有限公司 | Convertible support and detection device thereof |
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|---|---|
| CN105699147B (en) | 2019-04-12 |
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