CN105688268A

CN105688268A - Novel composite hemostatic material and preparation method of novel composite hemostatic material

Info

Publication number: CN105688268A
Application number: CN201610043001.3A
Authority: CN
Inventors: 梁奕福
Original assignee: Dongguan Da Qing Medical Devices Co Ltd; GUANGXI XINYE BIOLOGICAL TECHNOLOGY Co Ltd; Guangxi Daqing Biotechnology Co Ltd
Current assignee: Dongguan Da Qing Medical Devices Co Ltd; GUANGXI XINYE BIOLOGICAL TECHNOLOGY Co Ltd; Guangxi Daqing Biotechnology Co Ltd
Priority date: 2016-01-22
Filing date: 2016-01-22
Publication date: 2016-06-22

Abstract

The invention relates to a biomedical hemostatic material and in particular relates to a novel composite hemostatic material and a preparation method of the novel composite hemostatic material. The novel composite hemostatic material is prepared from micro-pore starch, collagen, hyaluronic acid, puffball mushroom spore powder and hemostatic traditional Chinese medicine extract, wherein the micro-pore starch has the effect of a molecular sieve and forms instant gel for blocking a blood vessel wound; the collagen can be rapidly combined with hemoglobin in blood to form hemostatic clots; the hyaluronic acid can absorb water in the blood instantly to form hydrogel, so that blood oozing is blocked; the four substances form a physical barrier for separating the wound, and have the effects of preventing sticking and instantly stopping bleeding; the hemostatic traditional Chinese medicine extract has the effects of stopping bleeding, resisting inflammation, resisting bacteria, alleviating pains, cooling blood, blooding stasis and promoting detumescence, promoting convergence, activating a blood coagulation system and promoting the healing of the wound. The five substances are organically combined, have a remarkable hemostatic effect, are used for stopping bleeding of various wounds and fresh tissue wound blood oozing, small blood vessel hemorrhage and hemorrhage regions, and preventing sticking, and are particularly suitable for the hemorrhage in surgeries.

Description

A kind of NEW TYPE OF COMPOSITE hemostatic material and preparation method thereof

Technical field

The present invention relates to biological medicine hemostatic material technical field, be specifically related to a kind of NEW TYPE OF COMPOSITE hemostatic material and preparation method thereof。

Background technology

Hemostasis is an important step in the operation of each section office of hospital, plays sizable effect for the success of operation and the safety of patient。Normal hemostatic mechanism mainly includes 3 aspects: the startup of the contraction of thin vessels, hematoblastic activation and blood coagulation system。Wherein, the contraction of thin vessels is the reaction the earliest of hemostasis after wound, and after vascular injury, vascular smooth muscle makes vasoconstriction, blood flow slow down by axon reflex, is conducive to blood coagulation and hemostasis。Platelet has the physiological function adhering to wound and foreign body surface, significant at primary hemostasis。The platelet adhered to is activated, and is likely to cause more platelet aggregation, and from discharging substantial amounts of coagulant thing inside it, and then stop blooding being partially formed platelet thrombus。Due to environmental factors or human body oneself factor, normal haemostasis mechanism can not save, for personal injury's large-area hemorrhage, the life that the wounded are about to disappear。Therefore, the hemostatic material of research hemostasis rapidly is important research topic。

Traditional hemostatic material mainly includes first-aid kit, tourniquet, gauze etc.。It is single that wound surface is only played physical protection effect, function and effect by these adjuvants, and haemostatic effect is not good enough, and is easily adhered wound surface。

Development along with technology, occur in that starch hemostatic material, Chinese invention patent application if application number is 200810032631.6 discloses the modified starch material of a kind of biocompatible hemostatic, anti, promoting healing, surgery closing, and this hemostatic material is esterification starch or the mixture of one or more esterifications, crosslinked starch。The molecular weight of modified starch is 15,000 ~ 10,000,000, and particle diameter is 10 ~ 1000 μm, and water absorption rate is 1 ~ 100。

But these current technology haemostatic effects are undesirable, anthemorrhagic speed is fast not, limits it in clinical extensive use。

Summary of the invention

Present invention aims to the deficiencies in the prior art, it is provided that a kind of anthemorrhagic speed is fast, the NEW TYPE OF COMPOSITE hemostatic material that is particularly well-suited in operation。

To achieve these goals, the present invention adopts the following technical scheme that

A kind of NEW TYPE OF COMPOSITE hemostatic material, described NEW TYPE OF COMPOSITE hemostatic material is made up of the raw material of following percentage by weight:

Micropore starch 20-40%

Collagen protein 10-20%

Hyaluronic acid 10-20%

Lasiosphaera Seu Calvatia spore powder 20-40%

Hemostatic of traditional herb medicine extract 10-30%。

Preferably, described NEW TYPE OF COMPOSITE hemostatic material is made up of the raw material of following percentage by weight:

Micropore starch 20-35%

Collagen protein 10-15%

Hyaluronic acid 10-20%

Lasiosphaera Seu Calvatia spore powder 20-35%

Hemostatic of traditional herb medicine extract 15-30%。

Preferably, the surface micropore diameter of described micropore starch is 6-15 μm, and the porosity of described micropore starch is more than 90%。

Preferably, collagen protein is mean molecule quantity is the daltonian pharmaceutical grade collagen protein of 500-2000, and described hyaluronic acid is mean molecule quantity is the daltonian pharmaceutical grade powder of 500-2000。

Preferably, described Lasiosphaera Seu Calvatia spore powder is that Lasiosphaera Seu Calvatia is peelled off exoperidium, digs out content and sifts out spore, crosses the Lasiosphaera Seu Calvatia spore powder that 100 orders obtain with upper screen cloth at 110-130 degree Celsius after being dry, pulverize by spore。

Preferably, described Hemostatic of traditional herb medicine extract is one or more mixture of Herba seu Radix Cirsii Japonici, Herba Cirsii extract, Ilex purpurea Hassk.[I.chinensis Sims, Radix Sangusorbae extract, Radix Boehmeriae extract, Madder extract, Lignum Dalbergiae Odoriferae extract, Pseudobulbus Bletillae (Rhizoma Bletillae) extract, Receptaculum nelumbinis extract, Nodus Nelumbinis Rhizomatis extract, Rhizoma Corydalis extract, Rhizoma Imperatae extract, Flos Sophorae extract, Cacumen Platycladi extract, Radix Notoginseng extract, Flos Celosiae Cristatae extract, Pollen Tyjphae extract, Crinis Carbonisatus extract, Herba Clinopodii extract, Herba Artemisiae Anomalae extract, Herba Agrimoniae extract, Folium Callicarpae Formosanae extract。Described Hemostatic of traditional herb medicine extract is commercially available。

Comparatively preferred, described Hemostatic of traditional herb medicine extract includes the raw material of following weight portion:

Herba seu Radix Cirsii Japonici 10-30 part

Herba Cirsii extract 20-40 part

Radix Sangusorbae extract 20-40 part

Radix Boehmeriae extract 10-30 part

Madder extract 5-10 part

Pseudobulbus Bletillae (Rhizoma Bletillae) extract 10-30 part

Receptaculum nelumbinis extract 20-40 part

Nodus Nelumbinis Rhizomatis extract 20-40 part

Rhizoma Imperatae extract 20-40 part

Flos Sophorae extract 10-30 part

Cacumen Platycladi extract 10-30 part

Radix Notoginseng extract 10-30 part

Pollen Tyjphae extract 10-30 part

Crinis Carbonisatus extract 5-10 part

Herba Clinopodii extract 5-10 part。

Being more highly preferred to, described Hemostatic of traditional herb medicine extract also includes the raw material of following weight portion:

Ilex purpurea Hassk.[I.chinensis Sims 10-30 part

Lignum Dalbergiae Odoriferae extract 5-10 part

Rhizoma Corydalis extract 20-40 part

Flos Celosiae Cristatae extract 10-30 part

Herba Artemisiae Anomalae extract 10-30 part

Herba Agrimoniae extract 20-40 part

Folium Callicarpae Formosanae extract 10-30 part。

The preparation method of a kind of NEW TYPE OF COMPOSITE hemostatic material, is mixed to get a kind of NEW TYPE OF COMPOSITE hemostatic material by micropore starch, collagen protein, hyaluronic acid, Lasiosphaera Seu Calvatia spore powder and Hemostatic of traditional herb medicine extract。

Preferably, ultrasonic activation 1-5 hour is continued after micropore starch, collagen protein, hyaluronic acid, Lasiosphaera Seu Calvatia spore powder and Hemostatic of traditional herb medicine extract mix homogeneously, ultrasonic activation can make each raw material mix evenly, and fraction Hemostatic of traditional herb medicine extract can enter in the duct of micropore starch, play the effect of lasting medicine release。

Described micropore starch is one or more the mixture in micropore potato starch, microporous tapioca, micropore sweet potato starch, micropore corn starch, micropore barley starch, micropore wheaten starch, micropore rice starch。

Micropore starch derives from natural plant starch, good biocompatibility, and without animal sources or humanized's composition, therefore allergy risk is little。Micropore starch has hydrophilic molecule filter screen effect, by assembling the solid constituent in blood, can form a kind of colloidal mixture, thus accelerating nature coagulation process around granule。These granules by allowing blood shrink produce quick and strong blood clot, can shorten the time for stopping blooding in operation greatly。Owing to starch is natural high molecular substance, can be absorbed by human body natural, because leading to complications without leaving at field of operation。

Micropore starch has the effect of molecular sieve, can quickly absorb the moisture in blood, is formed " instant gel ", mechanicalness can block blood vessel cut, and this physical barriers can isolate wound simultaneously, plays the effect of anti。The surface micropore diameter of preferred microporous starch is 6-15 μm, and the porosity of described micropore starch is more than 90%。Because affecting in the factor of the hemostatic capability of micropore starch, the size of water absorbing capacity is wherein one of key factor。And water absorbing capacity comes from, and the hole of micropore starch granule is how many and size。The present invention selects the micropore starch of appropriate microporous diameter and voidage, more can quickly absorb the moisture in blood, play the effect of quick-acting haemostatic powder。

Collagen protein can quickly the hemoglobin in blood be combined, and forms hemostasis grumeleuse, the closure blood capillary oozing of blood of mechanical-physical, after additionally collagen protein and wound are combined closely, can penetrate in the middle of cambium, and support during as Growth of Cells；Preferred collagen protein adopts mean molecule quantity to be the daltonian pharmaceutical grade collagen protein of 500-2000；Owing to the rank of collagen protein, molecular weight can directly affect absorption and the haemostatic effect of collagen protein, the collagen protein of 500-2000 dalton molecule weight range is best suitable for skin absorption, so the present invention selects mean molecule quantity to be the daltonian pharmaceutical grade collagen protein of 500-2000。

Hyaluronic acid demonstrates multiple important physiological function with molecular structure and the physicochemical property of its uniqueness in body, and the hyaluronic acid of the present invention has the adjustment permeability of blood vessel wall, Water-Electrolyte diffusion and operating, promotes the effects such as wound healing。Preferably clear matter acid adopts mean molecule quantity to be the daltonian pharmaceutical grade powder of 500-2000；Owing to hyaluronic purity, molecular weight can directly affect hyaluronic effect, so the present invention selects mean molecule quantity to be the daltonian pharmaceutical grade powder of 500-2000。

Lasiosphaera Seu Calvatia, nature and flavor are pungent, flat, nontoxic, containing Argine Monohydrochloride, the acid of cheese base, carbamide, ergosterol, lipoids, gemmatein etc., have antiinflammatory, antibacterial, the effect stopped blooding。Preferred Lasiosphaera Seu Calvatia spore powder refers to peels off exoperidium by Lasiosphaera Seu Calvatia, digs out content and sifts out spore, crosses the Lasiosphaera Seu Calvatia spore powder that 100 orders obtain with upper screen cloth at 110-130 degree Celsius after being dry, pulverize by spore。Through the above Lasiosphaera Seu Calvatia spore powder processed, there is better antibacterial, haemostatic effect。Lasiosphaera Seu Calvatia spore powder stores a large amount of micro-porous fibre, can absorb rapidly the moisture in blood, mechanicalness can block blood vessel cut and blood capillary oozing of blood, and this physical barriers can isolate wound simultaneously, plays the effect of instant hemostasis。

Hemostatic of traditional herb medicine extract of the present invention has following effect:

Radix Cirsii Japonici in Herba seu Radix Cirsii Japonici: nature and flavor sweet, bitter, cold；Gui Jing: GUIXIN, Liver Channel；Effect: cooling blood for hemostasis, removing blood stasis to reduce swelling；Cure mainly: for epistaxis, spit blood, hematuria, have blood in stool, metrorrhagia, traumatic hemorrhage, carbuncle sore tumefacting virus。

Herba Cirsii in Herba Cirsii extract, sweet, bitter, cold。GUIXIN, Liver Channel。Function with cure mainly: cooling blood for hemostasis, removing blood stasis to reduce swelling。For epistaxis, spit blood, hematuria, have blood in stool, metrostaxis, traumatic hemorrhage, carbuncle sore tumefacting virus。

Ilex purpurea Hassk.[I.chinensis Sims in Ilex purpurea Hassk.[I.chinensis Sims, has heat-clearing and toxic substances removing；Promoting tissue regeneration and ulcer healing；Effect of promoting blood circulation and hemostasis。

Radix Sanguisorbae in Radix Sangusorbae extract: there is cooling blood for hemostasis, heat-clearing and toxic substances removing, effect of detumescence sore, cure mainly haematemesis, spitting of blood, epistaxis, hematuria, have blood in stool, hemorrhoidal bleeding, dysentery, metrorrhagia, leucorrhea with red and white discharge, sore, carbuncle and painful swelling, eczema, pudendal pruritus, burn due to hot liquid or fire, the disease such as snake bite and insect sting。

Radix Boehmeriae in Radix Boehmeriae extract, sweet in the mouth is flat。Control calentura extreme thirst, mad greatly, stranguria with blood, the difficulty in urination, spit blood, hematochezia, leucorrhea with red and white discharge, erysipelas, carbuncle, traumatic injury, snake bite and insect sting。

Radix Rubiae in Madder extract: bitter, cold, return Liver Channel。There is removing heat from blood, hemostasis, blood stasis dispelling, stimulate the menstrual flow, antitussive, the effect eliminated the phlegm。For spitting blood, epistaxis, metrorrhagia, traumatic hemorrhage, amenorrhea stasis blocking, arthralgia, tumbling and swelling。

Lignum Dalbergiae Odoriferae in Lignum Dalbergiae Odoriferae extract, i.e. Huanghua Pear root heartwood, hyoscine。For good analgesics, control again knife injury hemorrhage。

Pseudobulbus Bletillae (Rhizoma Bletillae) in Pseudobulbus Bletillae (Rhizoma Bletillae) extract: pungent, bitter, sweet, puckery, it is slightly cold。Return lung, liver, stomach warp。There is the effect of hemostasis tonifying the lung, granulation promoting pain relieving。

Receptaculum Nelumbinis in Receptaculum nelumbinis extract: pained, temperature。There is repercussive, hemostasis, the effect dried。Control metrorrhagia, menorrhagia, vaginal bleeding during pregnancy hematochezia, abdominal pain due to blood stasis, puerperal placenta retention, dysentery, stranguria with blood, hemorrhoid proctoptosis, skin eczema。

Nodus Nelumbinis Rhizomatis in Nodus Nelumbinis Rhizomatis extract: cool, sweet。Health product clearing away heat and promoting production of body fluid, cooling blood for hemostasis;Ripe use strengthens the spleen and stomach, benefit blood granulation promoting。

Rhizoma Corydalis in Rhizoma Corydalis extract has invigorates blood circulation, circulation of qi promoting, effect of pain relieving。For the breast side of body, epigastric pain, obstruction of qi in the chest and cardialgia, amenorrhea dysmenorrhea, postpartum stagnation, tumbling and swelling。

Rhizoma Imperatae in Rhizoma Imperatae extract: nature and flavor are sweet, cold；Gui Jing: return lung, stomach, bladder warp；Effect: cooling blood for hemostasis, clearing away heat and promoting diuresis；Cure mainly: spit blood for heat in blood, epistaxis, hematuria, calentura excessive thirst, cough due to lung-heat, gastropyretic vomiting, jaundice due to damp-heat, edema oliguria, the puckery pain of pyretic stranguria。

Flos Sophorae in Flos Sophorae extract: bitter in the mouth, cold nature, returns liver, large intestine channel；Effect: cooling blood for hemostasis, purging liver-fire；Curing mainly: discharging fresh blood stool, hemorrhoidal bleeding, dysentery, hematuria, stranguria with blood, metrorrhagia, spit blood, epistaxis, liver-fire is had a headache, conjunctival congestion and swelling pain, sore throat, aphonia, ulcer sores。

Cacumen Platycladi in Cacumen Platycladi extract: nature and flavor are bitter, puckery, cold；Gui Jing: return lung, liver, spleen channel；Effect: cooling blood for hemostasis, preventing phlegm from forming and stopping coughing, growing and blacking hair；Cure mainly: be used for spitting blood, epistaxis, spitting of blood, have blood in stool, metrostaxis, cough due to lung-heat, heat in blood alopecia, early whitening of beard and hair。

Radix Notoginseng in Radix Notoginseng extract, nature and flavor, hardship sweet, micro-, temperature。Gui Jing, returns liver, stomach warp。Effect, dissipating blood stasis stops blooding, subduing swelling and relieving pain。Cure mainly, be used for spitting blood, spit blood, epistaxis, have blood in stool, metrorrhagia, traumatic hemorrhage, chest and abdomen twinge, tumbling and swelling。

Flos Celosiae Cristatae in Flos Celosiae Cristatae extract, sweet, cool, nontoxic。Enter kidney channel, removing heat from blood, hemostasis。Control anal fistula hematochezia, red white dysentery, spit blood, hemoptysis, stranguria with blood, women's metrorrhagia, leucorrhea with red and white discharge。

Pollen Typhae in Pollen Tyjphae extract, sweet in the mouth, micro-pungent；Property is put down, and has blood-cooling hemostatic；Effect of blood circulation and stasis-eliminating medicine, cures mainly haematemesis；Spitting of blood；Epistaxis；Standby dysentery；Have blood in stool；Metrorrhagia；Traumatic hemorrhage；Trusted subordinate's pain；Amenorrhea is suffered from abdominal pain；Puerperal the stasis of blood pain；Dysmenorrhea；Tumbling and swelling；Blood strangury and dry pain；Leukorrhagia；Congenital deformity of the tongue；Aphtha；Ear；Uncontrolled urination is itched。

Crinis Carbonisatus in Crinis Carbonisatus extract, nature and flavor, bitter, flat。Gui Jing returns liver, stomach warp。Effect astringing to arrest bleeding, blood stasis dispelling, diuresis。Cure mainly for spitting blood, spitting of blood, epistaxis, stranguria with blood, hematuria, have blood in stool, metrorrhagia, traumatic hemorrhage, dysuria。

Herba Clinopodii in Herba Clinopodii extract, micro-hardship, puckery, cool。Return Liver Channel。Effect: astringing to arrest bleeding。For metrorrhagia, hematuria, epistaxis, gingival hemorrhage, wound hemorrhage。

The Herba Artemisiae Anomalae of Herba Artemisiae Anomalae extract, meridian distribution of property and flavor: bitter。Temperature, GUIXIN, liver, spleen channel。Removing blood stasis stimulates the menstrual flow, healing up sore and subduing swelling。Control amenorrhea lump in the abdomen, chest and abdomen swelling and pain, puerperal blood stasis, traumatic injury, incised wound is hemorrhage, and carbuncle poison swells。

Herba Agrimoniae in Herba Agrimoniae extract, bitter, puckery, tepor, flat。GUIXIN, Liver Channel。Have and hold back hemostasis, dysentery relieving, effect of parasite killing。Be widely used in various hemorrhage card, for instance: haematemesis, hematuria, have blood in stool, metrorrhagia, spitting of blood, epistaxis, dysentery, impairment caused by overstrain takes off the diseases such as power, carbuncle, traumatic injury, wound hemorrhage。

Folium Callicarpae Formosanae in Folium Callicarpae Formosanae extract, pungent, bitter, flat。Return liver, spleen, stomach warp。Function: dissipating blood stasis stops blooding, reducing swelling and alleviating pain。Leaf: be used for spitting blood, spitting of blood, epistaxis, have blood in stool；External curing traumatic hemorrhage。Root: treating swelling and pain by traumatic injury, rheumatic ostalgia。

Above Chinese medicine all has the effect of hemostasis, single use can also, after compatibility, result of use is more preferably。Hemostatic of traditional herb medicine extract is carried out suitable compatibility by the present invention, existing make local vasoconstriction and stop blooding Chinese medicine such as Radix Notoginseng, Folium Callicarpae Formosanae, Herba Cirsii, also act on coagulation process, shorten clotting time, increase the Chinese medicine of platelet count and coagulant, such as Herba Agrimoniae, Folium Callicarpae Formosanae;Also have and strengthen PF3A, shorten thromboplastin and generate the time, such as the Pseudobulbus Bletillae (Rhizoma Bletillae);Have and increase thrombin in blood, such as Radix Notoginseng, Pollen Typhae, more have and can improve blood vessel wall function, strengthen the blood capillary resistance to damage, reduce the Chinese medicine such as Flos Sophorae of vascular permeability。Suppress the activity of plasmin (fibrinolysin): such as Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Cirsii Japonici, Herba Cirsii, Radix Sanguisorbae, Herba Agrimoniae。The one side of the existing promotion blood clottings such as Radix Notoginseng, Radix Rubiae, Pollen Typhae, also has and promotes clot dissolution effect, and its function has hemostasis and blood circulation promoting and blood stasis dispelling function concurrently, is conducive to hemostasis not stay the stasis of blood, is particularly well-suited to hemostatic material。To sum up, various Chinese medicines are learnt from other's strong points to offset one's weaknesses, and have played maximum effect, have hemostasis, antiinflammatory, antibacterial, analgesia, removing heat from blood, dissipating blood stasis for subsidence of swelling, convergence, activation blood coagulation system, promote the effect of wound healing。

The present invention compared with prior art, has the beneficial effects that:

(1) micropore starch of the present invention has the effect of molecular sieve, can quickly absorb the moisture in blood, is formed " instant gel ", mechanicalness closure blood vessel cut；Collagen protein can quickly the hemoglobin in blood be combined, and forms hemostasis grumeleuse, the closure blood capillary oozing of blood of mechanical-physical, after additionally collagen protein and wound are combined closely, can penetrate in the middle of cambium, and support during as Growth of Cells；Hyaluronic acid can immediately absorb moisture in blood and form hydrogel, thus blocking oozing of blood；Lasiosphaera Seu Calvatia spore powder stores a large amount of micro-porous fibre, can absorb rapidly the moisture in blood, can mechanicalness closure blood vessel cut and blood capillary oozing of blood。Micropore starch, collagen protein, hyaluronic acid, Lasiosphaera Seu Calvatia spore powder this be collectively forming physical barriers, wound can be isolated, play anti and the effect of instant hemostasis。Hemostatic of traditional herb medicine extract then has hemostasis, antiinflammatory, antibacterial, analgesia, removing heat from blood, dissipating blood stasis for subsidence of swelling, convergence, activation blood coagulation system, promote the effect of wound healing。Five kinds of materials of the present invention organically combine, and are obviously enhanced haemostatic effect, and anthemorrhagic speed significantly improves, and for the hemostasis of various wounds and flesh tissue blood oozing from the wound surface, the hemorrhage and hemorrhage district of thin vessels, Film with Preventing Adhesion, are particularly well-suited to the hemostasis in operation。

(2) the NEW TYPE OF COMPOSITE hemostatic material biocompatibility of the present invention meets GB/T16886.11-2011 requirement, and NEW TYPE OF COMPOSITE hemostatic material microbiological indicator meets GB15979-2002 requirement。

(3) present invention can stop blooding rapidly, and the gel viscosity of composition is higher, can clog damaged tissues and blood vessel, and the present invention is easily swelling and cleaning in water, and residue greatly reduces；The present invention is stable and is not easily decomposed, long shelf-life, has storage advantage。

Detailed description of the invention

Below in conjunction with embodiment, the present invention is further illustrated。

Embodiment 1。

A kind of NEW TYPE OF COMPOSITE hemostatic material, is made up of the raw material of following percentage by weight:

Micropore starch 20%

Collagen protein 20%

Hyaluronic acid 20%

Lasiosphaera Seu Calvatia spore powder 20%

Hemostatic of traditional herb medicine extract 10%。

The surface micropore diameter of described micropore starch is 6-15 μm, and the porosity of described micropore starch is more than 90%。

Described collagen protein is mean molecule quantity is 1000 daltonian pharmaceutical grade collagen protein, and described hyaluronic acid is mean molecule quantity is 500 daltonian pharmaceutical grade powder。

Described Lasiosphaera Seu Calvatia spore powder is that Lasiosphaera Seu Calvatia is peelled off exoperidium, digs out content and sifts out spore, crosses the Lasiosphaera Seu Calvatia spore powder that 100 orders obtain with upper screen cloth at 110 degrees Celsius after being dry, pulverize by spore。

Described Hemostatic of traditional herb medicine extract is 10 parts of Herba seu Radix Cirsii Japonicis and the mixture of 20 parts of Herba Cirsii extract compositions。

Above-mentioned micropore starch, collagen protein, hyaluronic acid, Lasiosphaera Seu Calvatia spore powder and Hemostatic of traditional herb medicine extract are mixed to get a kind of NEW TYPE OF COMPOSITE hemostatic material。

Embodiment 2。

Micropore starch 40%

Collagen protein 10%

Hyaluronic acid 10%

Lasiosphaera Seu Calvatia spore powder 25%

Hemostatic of traditional herb medicine extract 15%。

Described collagen protein is mean molecule quantity is 500 daltonian pharmaceutical grade collagen protein, and described hyaluronic acid is mean molecule quantity is 2000 daltonian pharmaceutical grade powder。

Described Lasiosphaera Seu Calvatia spore powder is that Lasiosphaera Seu Calvatia is peelled off exoperidium, digs out content and sifts out spore, crosses the Lasiosphaera Seu Calvatia spore powder that 100 orders obtain with upper screen cloth at 120 degrees Celsius after being dry, pulverize by spore。

The mixture that described Hemostatic of traditional herb medicine extract is made up of 20 parts of Radix Sangusorbae extracts, 40 portions of Radix Boehmeriae extracts, 30 portions of Flos Sophorae extracts, 30 portions of Pollen Tyjphae extracts, 25 portions of Herba Agrimoniae extracts。

Micropore starch, collagen protein, hyaluronic acid, Lasiosphaera Seu Calvatia spore powder and Hemostatic of traditional herb medicine extract are mixed to get a kind of NEW TYPE OF COMPOSITE hemostatic material。

Ultrasonic activation is continued 2 hours after micropore starch, collagen protein, hyaluronic acid, Lasiosphaera Seu Calvatia spore powder and Hemostatic of traditional herb medicine extract mix homogeneously。

Embodiment 3。

Micropore starch 30%

Collagen protein 15%

Hyaluronic acid 10%

Lasiosphaera Seu Calvatia spore powder 20%

Hemostatic of traditional herb medicine extract 25%。

Described collagen protein is mean molecule quantity is 2000 daltonian pharmaceutical grade collagen protein, and described hyaluronic acid is mean molecule quantity is 1000 daltonian pharmaceutical grade powder。

Described Lasiosphaera Seu Calvatia spore powder is that Lasiosphaera Seu Calvatia is peelled off exoperidium, digs out content and sifts out spore, crosses the Lasiosphaera Seu Calvatia spore powder that 100 orders obtain with upper screen cloth at 130 degrees Celsius after being dry, pulverize by spore。

Described Hemostatic of traditional herb medicine extract is the mixture of 10 parts of Radix Sangusorbae extracts, 20 parts of Madder extracts, 20 parts of Rhizoma Imperatae extracts, 10 parts of Flos Celosiae Cristatae extracts, 20 parts of Herba Clinopodii extracts, 5 parts of Herba Artemisiae Anomalae extracts, 10 parts of Folium Callicarpae Formosanae extract compositions。

Ultrasonic activation is continued 5 hours after micropore starch, collagen protein, hyaluronic acid, Lasiosphaera Seu Calvatia spore powder and Hemostatic of traditional herb medicine extract mix homogeneously。

Embodiment 4。

Micropore starch 35%

Collagen protein 10%

Hyaluronic acid 15%

Lasiosphaera Seu Calvatia spore powder 20%

Hemostatic of traditional herb medicine extract 20%。

Described collagen protein is mean molecule quantity is 700 daltonian pharmaceutical grade collagen protein, and described hyaluronic acid is mean molecule quantity is 1300 daltonian pharmaceutical grade powder。

Described Hemostatic of traditional herb medicine extract is made up of the raw material of following weight portion:

Herba seu Radix Cirsii Japonici 10 parts

Herba Cirsii extract 40 parts

Radix Sangusorbae extract 20 parts

Radix Boehmeriae extract 30 parts

Madder extract 5 parts

Pseudobulbus Bletillae (Rhizoma Bletillae) extract 30 parts

Receptaculum nelumbinis extract 20 parts

Nodus Nelumbinis Rhizomatis extract 40 parts

Rhizoma Imperatae extract 20 parts

Flos Sophorae extract 30 parts

Cacumen Platycladi extract 10 parts

Radix Notoginseng extract 30 parts

Pollen Tyjphae extract 10 parts

Crinis Carbonisatus extract 10 parts

Herba Clinopodii extract 5 parts。

Ultrasonic activation is continued 3 hours after micropore starch, collagen protein, hyaluronic acid, Lasiosphaera Seu Calvatia spore powder and Hemostatic of traditional herb medicine extract mix homogeneously。

Embodiment 5。

Micropore starch 40%

Collagen protein 10%

Hyaluronic acid 10%

Lasiosphaera Seu Calvatia spore powder 30%

Hemostatic of traditional herb medicine extract 10%。

Described collagen protein is mean molecule quantity is 600 daltonian pharmaceutical grade collagen protein, and described hyaluronic acid is mean molecule quantity is 1700 daltonian pharmaceutical grade powder。

Described Hemostatic of traditional herb medicine extract includes the raw material of following weight portion:

Herba seu Radix Cirsii Japonici 30 parts

Herba Cirsii extract 20 parts

Radix Sangusorbae extract 40 parts

Radix Boehmeriae extract 10 parts

Madder extract 10 parts

Pseudobulbus Bletillae (Rhizoma Bletillae) extract 10 parts

Receptaculum nelumbinis extract 40 parts

Nodus Nelumbinis Rhizomatis extract 20 parts

Rhizoma Imperatae extract 40 parts

Flos Sophorae extract 10 parts

Cacumen Platycladi extract 30 parts

Radix Notoginseng extract 10 parts

Pollen Tyjphae extract 30 parts

Crinis Carbonisatus extract 5 parts

Herba Clinopodii extract 10 parts。

Ultrasonic activation is continued 4 hours after micropore starch, collagen protein, hyaluronic acid, Lasiosphaera Seu Calvatia spore powder and Hemostatic of traditional herb medicine extract mix homogeneously。

Embodiment 6。

Micropore starch 35%

Collagen protein 15%

Hyaluronic acid 15%

Lasiosphaera Seu Calvatia spore powder 20%

Hemostatic of traditional herb medicine extract 15%。

Described collagen protein is mean molecule quantity is 900 daltonian pharmaceutical grade collagen protein, and described hyaluronic acid is mean molecule quantity is 600 daltonian pharmaceutical grade powder。

Herba seu Radix Cirsii Japonici 30 parts

Herba Cirsii extract 20 parts

Radix Sangusorbae extract 20 parts

Radix Boehmeriae extract 30 parts

Madder extract 5 parts

Pseudobulbus Bletillae (Rhizoma Bletillae) extract 10 parts

Receptaculum nelumbinis extract 40 parts

Nodus Nelumbinis Rhizomatis extract 20 parts

Rhizoma Imperatae extract 20 parts

Flos Sophorae extract 30 parts

Cacumen Platycladi extract 10 parts

Radix Notoginseng extract 10 parts

Pollen Tyjphae extract 30 parts

Crinis Carbonisatus extract 5 parts

Herba Clinopodii extract 5 parts。

Ilex purpurea Hassk.[I.chinensis Sims 30 parts

Lignum Dalbergiae Odoriferae extract 5 parts

Rhizoma Corydalis extract 20 parts

Flos Celosiae Cristatae extract 30 parts

Herba Artemisiae Anomalae extract 10 parts

Herba Agrimoniae extract 20 parts

Folium Callicarpae Formosanae extract 30 parts。

Embodiment 7。

Micropore starch 30%

Collagen protein 10%

Hyaluronic acid 10%

Lasiosphaera Seu Calvatia spore powder 30%

Hemostatic of traditional herb medicine extract 20%。

Described collagen protein is mean molecule quantity is 800 daltonian pharmaceutical grade collagen protein, and described hyaluronic acid is mean molecule quantity is 1400 daltonian pharmaceutical grade powder。

Herba seu Radix Cirsii Japonici 20 parts

Herba Cirsii extract 30 parts

Radix Sangusorbae extract 30 parts

Radix Boehmeriae extract 20 parts

Madder extract 8 parts

Pseudobulbus Bletillae (Rhizoma Bletillae) extract 20 parts

Receptaculum nelumbinis extract 30 parts

Nodus Nelumbinis Rhizomatis extract 30 parts

Rhizoma Imperatae extract 30 parts

Flos Sophorae extract 20 parts

Cacumen Platycladi extract 20 parts

Radix Notoginseng extract 20 parts

Pollen Tyjphae extract 20 parts

Crinis Carbonisatus extract 8 parts

Herba Clinopodii extract 8 parts。

Ilex purpurea Hassk.[I.chinensis Sims 20 parts

Lignum Dalbergiae Odoriferae extract 8 parts

Rhizoma Corydalis extract 25 parts

Flos Celosiae Cristatae extract 15 parts

Herba Artemisiae Anomalae extract 25 parts

Herba Agrimoniae extract 30 parts

Folium Callicarpae Formosanae extract 25 parts。

Embodiment 8。

Micropore starch 25%

Collagen protein 20%

Hyaluronic acid 15%

Lasiosphaera Seu Calvatia spore powder 25%

Hemostatic of traditional herb medicine extract 15%。

Herba seu Radix Cirsii Japonici 10 parts

Herba Cirsii extract 40 parts

Radix Sangusorbae extract 40 parts

Radix Boehmeriae extract 10 parts

Madder extract 10 parts

Pseudobulbus Bletillae (Rhizoma Bletillae) extract 30 parts

Receptaculum nelumbinis extract 20 parts

Nodus Nelumbinis Rhizomatis extract 40 parts

Rhizoma Imperatae extract 40 parts

Flos Sophorae extract 10 parts

Cacumen Platycladi extract 30 parts

Radix Notoginseng extract 30 parts

Pollen Tyjphae extract 10 parts

Crinis Carbonisatus extract 10 parts

Herba Clinopodii extract 10 parts。

Ilex purpurea Hassk.[I.chinensis Sims 10 parts

Lignum Dalbergiae Odoriferae extract 10 parts

Rhizoma Corydalis extract 40 parts

Flos Celosiae Cristatae extract 10 parts

Herba Artemisiae Anomalae extract 30 parts

Herba Agrimoniae extract 40 parts

Folium Callicarpae Formosanae extract 10 parts。

Stop blooding potency assay and compare:

Laboratory animal and packet adult healthy rabbit 50 (Guangxi Medical University animal experimental center provide), body weight 2.5-3kg, male and female are not limit, experimental group and matched group, often organize each 25, in each pressing time section each 5。Every rabbit both sides kidney makes long 1cm, the deep hemorrhage wound surface of 0.5cm size, with the hemostatic material of embodiment of the present invention 1-8, hemorrhage wound surface is stopped blooding at random, with Arista styptic powder to the hemostasis of opposite side wound surface for comparison。Experimental group is the application of embodiment of the present invention 1-8 hemostatic material, and matched group is the application of Arista styptic powder。

Experimental technique and observation index

Hemorrhage Model makes: 3% pentobarbital sodium injection (30-40mg/kg) is anaesthetized along family's rabbit ear along intravenous injection, laboratory table fixes rabbit, sterile working successively opens abdomen, expose rabbit kidney, two longitudinally cutting wounds are made at kidney diverse location at random with knife blade, it is about 1cm, deep about 0.5cm, after cutting part blood blots with the dry gauze claiming weight, with the hemostatic material of 1g embodiment of the present invention 1-8, hemorrhage wound surface is stopped blooding at random, with 1gArista powder to the hemostasis of opposite side wound surface for comparison, use gauze pressing wound surface, oppress 10s respectively, 20s, 30s, 40s, 50s also records amount of bleeding and the bleeding stopping period of each time group。

Hemorrhage index observing

Bleeding stopping period kidney hemorrhagic disease wound surface is observed: calculates and starts to be applied to wound surface to wound surface blood from the present invention or Arista styptic powder and ooze out stopping or the red dye area of styptic powder granule no longer expands and is recorded as bleeding stopping period。

Dry gauze weight before amount of bleeding is stopped blooding with analytical balance accurate weighing experimental group in advance and matched group and after hemostasis, calculate blood volume (after amount of bleeding=hemostasis weight/blood specific gravity before weight-hemostasis) respectively。

Result: general character and ultrastructure: the hemostatic material of the present invention is white powder granule, perusal, granular size is homogeneous, grain structure relatively Arista styptic powder is fine and smooth, under scanning electron microscope, starch granules diameter is about 20 μm, and there is multiple pore passage structure inside, aperture is about 1.5 μm, and pore size is essentially identical。Arista styptic powder is white powder granule, and under scanning electron microscope, visible particle surface is made up of multiple cracks, and without pore passage structure, particle volume is about 1-2 times of the present invention。

Experimental result shows, when oppressing 10s, 20s, kidney hemorrhagic disease amount is big, and the bleeding time is long。Pressing time is more than after 30s, and amount of bleeding and bleeding time change are obvious, and both substantially shorten, and after compressing 40s, 50s, hemorrhage index amplitude of variation reduces further。Experimental data shows: experimental group and matched group are both needed to compressing more than 30s, and haemostatic effect could embody。Table 1 is in different pressing time, the hemostatic material of embodiment of the present invention 1-8 and the comparison of Arista styptic powder amount of bleeding and bleeding stopping period。

Table 1

。

Can be seen that haemostatic effect of the present invention is substantially better than Arista styptic powder by table 1。

Haemostatic effect gross examination of skeletal muscle

After exposing kidney, after otch is performed an operation on normal kidney surface, blood is gushed out rapidly, after blotting blood with dry gauze, quickly spills the styptic powder of the 1g present invention, and hemorrhage district starch is reddened immediately by white, and dry gauze continues to oppress。20s compressing group, removes gauze, and hemorrhage wound surface has a small amount of blood to ooze out, and the red dye area of styptic powder of the present invention slowly expands；30s compressing group, the hemorrhage basic stopping of kidney otch, without obvious oozing phenomenon；40s and 50s compressing group, after removing gauze, it is seen that the styptic powder of the present invention attaches to cut surface, stopped bleeding in the pasty state。

Finally should be noted that; above example is only in order to illustrate technical scheme; but not limiting the scope of the invention; although having made to explain to the present invention with reference to preferred embodiment; it will be understood by those within the art that; technical scheme can be modified or equivalent replacement, without deviating from the spirit and scope of technical solution of the present invention。

Claims

1. a NEW TYPE OF COMPOSITE hemostatic material, it is characterised in that: this NEW TYPE OF COMPOSITE hemostatic material is made up of the raw material of following percentage by weight:

Micropore starch 20-40%

Collagen protein 10-20%

Hyaluronic acid 10-20%

Lasiosphaera Seu Calvatia spore powder 20-40%

Hemostatic of traditional herb medicine extract 10-30%。

2. a kind of NEW TYPE OF COMPOSITE hemostatic material according to claim 1, it is characterised in that: this NEW TYPE OF COMPOSITE hemostatic material is made up of the raw material of following percentage by weight:

Micropore starch 20-35%

Collagen protein 10-15%

Hyaluronic acid 10-20%

Lasiosphaera Seu Calvatia spore powder 20-35%

Hemostatic of traditional herb medicine extract 15-30%。

3. a kind of NEW TYPE OF COMPOSITE hemostatic material according to claim 1, it is characterised in that: the surface micropore diameter of described micropore starch is 6-15 μm, and the porosity of described micropore starch is more than 90%。

4. a kind of NEW TYPE OF COMPOSITE hemostatic material according to claim 1, it is characterized in that: described collagen protein is mean molecule quantity is the daltonian pharmaceutical grade collagen protein of 500-2000, described hyaluronic acid is mean molecule quantity is the daltonian pharmaceutical grade powder of 500-2000。

5. a kind of NEW TYPE OF COMPOSITE hemostatic material according to claim 1, it is characterized in that: described Lasiosphaera Seu Calvatia spore powder is that Lasiosphaera Seu Calvatia is peelled off exoperidium, dig out content and sift out spore, after being dry, pulverize at 110-130 degree Celsius by spore, cross the Lasiosphaera Seu Calvatia spore powder that 100 orders obtain with upper screen cloth。

6. a kind of NEW TYPE OF COMPOSITE hemostatic material according to claim 1, it is characterized in that: described Hemostatic of traditional herb medicine extract is Herba seu Radix Cirsii Japonici, Herba Cirsii extract, Ilex purpurea Hassk.[I.chinensis Sims, Radix Sangusorbae extract, Radix Boehmeriae extract, Madder extract, Lignum Dalbergiae Odoriferae extract, Pseudobulbus Bletillae (Rhizoma Bletillae) extract, Receptaculum nelumbinis extract, Nodus Nelumbinis Rhizomatis extract, Rhizoma Corydalis extract, Rhizoma Imperatae extract, Flos Sophorae extract, Cacumen Platycladi extract, Radix Notoginseng extract, Flos Celosiae Cristatae extract, Pollen Tyjphae extract, Crinis Carbonisatus extract, Herba Clinopodii extract, Herba Artemisiae Anomalae extract, Herba Agrimoniae extract, one or more mixture of Folium Callicarpae Formosanae extract。

7. a kind of NEW TYPE OF COMPOSITE hemostatic material according to claim 6, it is characterised in that: described Hemostatic of traditional herb medicine extract includes the raw material of following weight portion:

Herba seu Radix Cirsii Japonici 10-30 part

Herba Cirsii extract 20-40 part

Radix Sangusorbae extract 20-40 part

Radix Boehmeriae extract 10-30 part

Madder extract 5-10 part

Pseudobulbus Bletillae (Rhizoma Bletillae) extract 10-30 part

Receptaculum nelumbinis extract 20-40 part

Nodus Nelumbinis Rhizomatis extract 20-40 part

Rhizoma Imperatae extract 20-40 part

Flos Sophorae extract 10-30 part

Cacumen Platycladi extract 10-30 part

Radix Notoginseng extract 10-30 part

Pollen Tyjphae extract 10-30 part

Crinis Carbonisatus extract 5-10 part

Herba Clinopodii extract 5-10 part。

8. a kind of NEW TYPE OF COMPOSITE hemostatic material according to claim 7, it is characterised in that: described Hemostatic of traditional herb medicine extract also includes the raw material of following weight portion:

Ilex purpurea Hassk.[I.chinensis Sims 10-30 part

Lignum Dalbergiae Odoriferae extract 5-10 part

Rhizoma Corydalis extract 20-40 part

Flos Celosiae Cristatae extract 10-30 part

Herba Artemisiae Anomalae extract 10-30 part

Herba Agrimoniae extract 20-40 part

Folium Callicarpae Formosanae extract 10-30 part。

9. the preparation method of a kind of NEW TYPE OF COMPOSITE hemostatic material described in claim 1, it is characterised in that: micropore starch, collagen protein, hyaluronic acid, Lasiosphaera Seu Calvatia spore powder and Hemostatic of traditional herb medicine extract are mixed to get a kind of NEW TYPE OF COMPOSITE hemostatic material。

10. the preparation method of a kind of NEW TYPE OF COMPOSITE hemostatic material according to claim 9, it is characterised in that: continue ultrasonic activation 1-5 hour after micropore starch, collagen protein, hyaluronic acid, Lasiosphaera Seu Calvatia spore powder and Hemostatic of traditional herb medicine extract mix homogeneously。