CN105664230A

CN105664230A - Porous fiber intraoperative composite hemostatic material and preparation method thereof

Info

Publication number: CN105664230A
Application number: CN201610042995.7A
Authority: CN
Inventors: 梁奕福
Original assignee: Dongguan Da Qing Medical Devices Co Ltd; GUANGXI XINYE BIOLOGICAL TECHNOLOGY Co Ltd; Guangxi Daqing Biotechnology Co Ltd
Current assignee: Dongguan Da Qing Medical Devices Co Ltd; GUANGXI XINYE BIOLOGICAL TECHNOLOGY Co Ltd; Guangxi Daqing Biotechnology Co Ltd
Priority date: 2016-01-22
Filing date: 2016-01-22
Publication date: 2016-06-15

Abstract

The invention relates to bio-medicine hemostatic materials, in particular to a porous fiber intraoperative composite hemostatic material and a preparation method thereof.The porous fiber intraoperative composite hemostatic material is composed of microporous starch, hyaluronic acid, puffball spore powder and hemostatic traditional Chinese medicine extract, the microporous starch has effect as a molecular sieve and can form instant gel to mechanically plug a blood vessel break, the hyaluronic acid can immediately absorb water in blood to form hydrogel so as to plug errhysis, and the puffball spore powder stores a lot of microporous fibers and can mechanically plug the blood vessel break.The three substances form a physical barrier to isolate a wound so as to play a role in adhesion preventing and immediate hemostasis, and the hemostatic traditional Chinese medicine extract has effect of stopping bleeding, resisting inflammation and bacteria, easing pain, cooling blood, dispersing stasis to diminish swelling, astringing, activating a coagulative system and promoting wound healing.The four substances are organically combined, so that hemostatic effect is enhanced remarkably.The porous fiber intraoperative composite hemostatic material is used for hemostasis of errhysis of various wounds and fresh tissue wounds, bleeding of small blood vessels and bleeding areas to prevent adhesion and is especially suitable for intraoperative hemostasis.

Description

Compound hemostatic material and its preparation method in a kind of multiporous fiber art

Technical field

The present invention relates to biological medicine hemostatic material technical field, it is specifically related to compound hemostatic material and its preparation method in a kind of multiporous fiber art.

Background technology

Hemostasis is an important step during each section office of hospital perform the operation, and plays sizable effect for the success of operation and the safety of patient. Normal hemostatic mechanism mainly comprises 3 aspects: the startup of the contraction of thin vessels, the activation of thrombocyte and blood coagulation system. Wherein, the contraction of thin vessels is the reaction the earliest of hemostasis after wound, and after vascular injury, vascular smooth muscle makes vasoconstriction, blood flow slow down by axon reflex, is conducive to blood coagulation and hemostasis. Thrombocyte has the physiological function adhering to wound and foreign matter surface, significant at primary hemostasis. The thrombocyte adhered to is activated, and may cause more platelet aggregation, and discharges a large amount of short solidifying thing from its inside, and then stops blooding being partially formed thrombocyte thrombus. Due to environmental factors or human body oneself factor, normal hemostatic mechanism can not save, for personal injury's large-area hemorrhage, the life that the wounded are about to disappearance. Therefore, the hemostatic material that research is stopped blooding rapidly is important research topic.

Traditional hemostatic material mainly comprises medicine bag, tourniquet, gauze etc. It is single that the surface of a wound is only played physical protection effect, function and effect by these auxiliary materials, and haemostatic effect is not good enough, and is easily adhered the surface of a wound.

Along with the development of technology, starch hemostatic material has occurred, such as the modified starch material that Chinese invention patent application discloses a kind of biocompatible hemostatic, anti, promoting healing, surgery are closed that application number is 200810032631.6, this hemostatic material is the mixture of esterification starch or one or more esterifications, cross-linking starch.The molecular weight of treated starch is 15,000 ~ 10,000,000, and particle diameter is 10 ~ 1000 μm, and water-intake rate is 1 ~ 100.

But these current technology haemostatic effects are undesirable, anthemorrhagic speed is fast not, limits it in clinical widespread use.

Summary of the invention

It is an object of the invention to for the deficiencies in the prior art, it is provided that a kind of anthemorrhagic speed is fast, haemostatic effect is specially adapted to well compound hemostatic material in the multiporous fiber art in performing the operation.

In order to realize above-mentioned purpose, the present invention adopts following technical scheme:

Compound hemostatic material in a kind of multiporous fiber art, in described multiporous fiber art, compound hemostatic material is made up of the raw material of following weight percent:

Micropore starch 20-40%

Hyaluronic acid 20-40%

Lasiosphaera fenzlii spore powder 20-40%

Hemostatic of traditional herb medicine extract 10-30%.

Preferably, in described multiporous fiber art, compound hemostatic material is made up of the raw material of following weight percent:

Micropore starch 20-35%

Hyaluronic acid 20-35%

Lasiosphaera fenzlii spore powder 20-35%

Hemostatic of traditional herb medicine extract 15-30%.

Preferably, the surface micropore diameter of described micropore starch is 6-15 μm, and the porosity of described micropore starch is more than 90%.

Preferably, the pharmaceutical grade powder of hyaluronic acid to be molecular-weight average be 500-2000 dalton.

Preferably, described Lasiosphaera fenzlii spore powder is that Lasiosphaera fenzlii is peelled off outer foreskin, digs out content and sifts out spore, by spore 110-130 degree Celsius of drying, crosses the Lasiosphaera fenzlii spore powder that 100 orders obtain with upper screen cloth after pulverizing.

Preferably, described Hemostatic of traditional herb medicine extract is one or more mixture of Herba seu Radix Cirsii Japonici, Herba Cirsii extract, SIJIQING extract, Radix Sangusorbae extract, Ramie Root extract, Madder extract, Lignum Dalbergiae Odoriferae extract, Pseudobulbus Bletillae (Rhizoma Bletillae) extract, Receptaculum nelumbinis extract, rhizoma nelumbinis extract, Rhizoma Corydalis extract, Rhizoma Imperatae extract, Flos Sophorae extract, Cacumen Platycladi extract, Radix Notoginseng extract, Flos Celosiae Cristatae extract, Pollen Tyjphae extract, Crinis Carbonisatus extract, Herba Clinopodii extract, Herba Artemisiae Anomalae extract, Herba Agrimoniae extract, Folium Callicarpae Formosanae extract. Described Hemostatic of traditional herb medicine extract is commercially available.

Comparatively preferred, described Hemostatic of traditional herb medicine extract comprises the raw material of following weight part:

Herba seu Radix Cirsii Japonici 10-30 part

Herba Cirsii extract 20-40 part

Radix Sangusorbae extract 20-40 part

Ramie Root extract 10-30 part

Madder extract 5-10 part

Pseudobulbus Bletillae (Rhizoma Bletillae) extract 10-30 part

Receptaculum nelumbinis extract 20-40 part

Rhizoma nelumbinis extract 20-40 part

Rhizoma Imperatae extract 20-40 part

Flos Sophorae extract 10-30 part

Cacumen Platycladi extract 10-30 part

Radix Notoginseng extract 10-30 part

Pollen Tyjphae extract 10-30 part

Crinis Carbonisatus extract 5-10 part

Herba Clinopodii extract 5-10 part.

More preferred, described Hemostatic of traditional herb medicine extract also comprises the raw material of following weight part:

SIJIQING extract 10-30 part

Lignum Dalbergiae Odoriferae extract 5-10 part

Rhizoma Corydalis extract 20-40 part

Flos Celosiae Cristatae extract 10-30 part

Herba Artemisiae Anomalae extract 10-30 part

Herba Agrimoniae extract 20-40 part

Folium Callicarpae Formosanae extract 10-30 part.

A preparation method for compound hemostatic material in multiporous fiber art, is mixed to get compound hemostatic material in a kind of multiporous fiber art by micropore starch, hyaluronic acid, Lasiosphaera fenzlii spore powder and Hemostatic of traditional herb medicine extract.

Preferably, micropore starch, hyaluronic acid, Lasiosphaera fenzlii spore powder and Hemostatic of traditional herb medicine extract continue ultrasonic activation 1-5 hour after mixing, ultrasonic activation can make that each raw material mixes evenly, and small portion Hemostatic of traditional herb medicine extract can enter into the duct of micropore starch, play the effect of lasting medicine release.

Described micropore starch is one or more the mixture in micropore yam starch, microporous tapioca, micropore sweet potato starch, micropore W-Gum, micropore barley starch, micropore wheat starch, micropore Starch rice.

Micropore starch derives from natural plant starch, good biocompatibility, and not containing animal source or humanized's composition, therefore irritated risk is little. Micropore starch has hydrophilic molecule filter screen effect, by the solids component assembled in blood, can form a kind of colloidal mixture, thus accelerate nature coagulation process around particle. These particles produce quick and strong blood clot by allowing blood shrink, and shorten the time for stopping blooding in operation greatly. Owing to starch is natural high molecular substance, can be absorbed by human body natural, thus can not leave in operation district and lead to complications.

Micropore starch has the effect of molecular sieve, can moisture in fast absorption blood, formed " instant gel ", can mechanicalness shutoff blood vessel cut, this physical barriers can isolate wound simultaneously, plays the effect of anti. The surface micropore diameter of preferred microporous starch is 6-15 μm, and the porosity of described micropore starch is more than 90%. Because affecting in the factor of the hemostatic capability of micropore starch, the size of water-retaining capacity is wherein one of important factor. And water-retaining capacity comes from, and the hole of micropore starch particle is how many and size. The present invention selects the micropore starch of suitable micro-pore diameter and voidage, more can moisture in fast absorption blood, play the effect of quick-acting haemostatic powder.

Hyaluronic acid demonstrates multiple important physiological function with the molecular structure of its uniqueness and physico-chemical property in body, and the hyaluronic acid of the present invention has the permeability, Water-Electrolyte diffusion and the effect such as running, promotion wound healing that regulate vessel wall. Preferably transparent matter acid employing molecular-weight average is the pharmaceutical grade powder of 500-2000 dalton; Owing to the purity of hyaluronic acid, molecular weight can directly affect the result of use of hyaluronic acid, so the present invention selects molecular-weight average to be the pharmaceutical grade powder of 500-2000 dalton.

Lasiosphaera fenzlii, nature and flavor are pungent, flat, nontoxic, containing Argine Monohydrochloride, the acid of cheese base, urea, ergosterol, lipoids, calvacin etc., have anti-inflammatory, antibacterial, the effect stopped blooding. Preferred Lasiosphaera fenzlii spore powder refers to peels off outer foreskin by Lasiosphaera fenzlii, digs out content and sifts out spore, by spore 110-130 degree Celsius of drying, crosses the Lasiosphaera fenzlii spore powder that 100 orders obtain with upper screen cloth after pulverizing. Lasiosphaera fenzlii spore powder through above process has better antibacterial, haemostatic effect. Lasiosphaera fenzlii spore powder stores a large amount of micro-multiporous fiber, can the moisture in absorbing blood rapidly, mechanicalness shutoff blood vessel cut and capillary vessel can ooze blood, this physical barriers can isolate wound simultaneously, plays the effect of instant hemostasis.

Hemostatic of traditional herb medicine extract of the present invention has following effect:

Setose thistle in Herba seu Radix Cirsii Japonici: nature and flavor are sweet, bitter, cool; Gui Jing: the thoughts of returning home, Liver Channel; Effect: cooling blood for hemostasis, removing blood stasis to reduce swelling; Curing mainly: for bleeding from five sense organs or subcutaneous tissue, spit blood, hematuria, has blood in stool, uterine bleeding, traumatic hemorrhage, carbuncle sore tumefacting virus.

Field thistle in Herba Cirsii extract, sweet, bitter, cool. The thoughts of returning home, Liver Channel. Function with cure mainly: cooling blood for hemostasis, removing blood stasis to reduce swelling. For bleeding from five sense organs or subcutaneous tissue, spitting blood, hematuria, has blood in stool, metrostaxis, traumatic hemorrhage, carbuncle sore tumefacting virus.

Folium ilicis chinensis in SIJIQING extract, has clearing heat and detoxicating; Promoting tissue regeneration and ulcer healing;Effect of promoting blood circulation and hemostasis.

Garden burnet in Radix Sangusorbae extract: have cooling blood for hemostasis, clearing heat and detoxicating, effect of detumescence sore, cures mainly haematemesis, and spitting of blood, bleeding from five sense organs or subcutaneous tissue, hematuria, has blood in stool, hemorrhoid blood, bloody flux, uterine bleeding, leucorrhea with red and white discharge, sore, carbuncle and painful swelling, eczema, pruritus vulvae, burn due to hot liquid or fire, the diseases such as snake bite and insect sting.

Ramie Root in Ramie Root extract, taste is sweet, flat. Control pyreticosis greatly yearningly, mad greatly, blood drench, the retention of urine, spit blood, lower blood, leucorrhea with red and white discharge, erysipelas, carbuncle swell, wound, snake bite and insect sting.

Madder in Madder extract: bitter, cold, return Liver Channel. Having cool blood, hemostasis, the stasis of blood of dispelling, stimulates the menstrual flow, antibechic, the effect eliminated the phlegm. For spitting blood, bleeding from five sense organs or subcutaneous tissue, uterine bleeding, traumatic hemorrhage, through closing stasis blocking, arthralgia, tumbling and swelling.

I.e. chrysanthemum pears root heartwood falls perfume (or spice), in Lignum Dalbergiae Odoriferae extract, for medicinal. For good analgesic agent, control again knife wound hemorrhage.

White in Pseudobulbus Bletillae (Rhizoma Bletillae) extract and: pungent, bitter, sweet, puckery, micro-cold. Return lung, liver, stomach warp. There is the effect of hemostasis tonifying lung, myogenic pain relieving.

Lotus pod in Receptaculum nelumbinis extract: pained, temperature. There is the stasis of blood that disappears, hemostasis, the effect dried. Controlling metrorrhagia, menorrhagia, blood under threatened abortion, hemostasis is suffered from abdominal pain, postpartum retention of placenta, bloody flux, blood drenches, hemorrhoid prolapse of the anus, and skin wets sore.

Rhizoma nelumbinis in rhizoma nelumbinis extract: cool, sweet. Raw product clearing heat and promoting fluid, cooling blood for hemostasis; Ripe help taste, benefit blood myogenic.

Yanhusuo in Rhizoma Corydalis extract has invigorates blood circulation, promoting the circulation of qi, effect of pain relieving. For chest the side of body, epigastric pain, Obstruction of qi in the chest and cardialgia, through closing dysmenorrhoea, postpartum stasis blocking, tumbling and swelling.

Rhizome of Lalang Grass in Rhizoma Imperatae extract: nature and flavor are sweet, cold; Gui Jing: return lung, stomach, bladder warp; Effect: cooling blood for hemostasis, clearing away heat and promoting diuresis; Curing mainly: spit blood for blood-head, bleeding from five sense organs or subcutaneous tissue, hematuria, pyreticosis polydipsia, cough due to lung-heat, gastropyretic vomiting, jaundice due to damp-heat, oedema urine is few, and heat drenches puckery pain.

Sophora flower in Flos Sophorae extract: bitter, cold nature, returns liver, large intestine channel; Effect: cooling blood for hemostasis, clearing liver-fire; Curing mainly: discharging fresh blood stool, hemorrhoid blood, bloody flux, hematuria, blood drenches, uterine bleeding, spits blood, bleeding from five sense organs or subcutaneous tissue, and irascibility is had a headache, conjunctival congestion with pain and swelling of the eye, larynx numbness, aphonia, ulcer sores.

Leafy twigs of Oriental Arborvitae in Cacumen Platycladi extract: nature and flavor are bitter, puckery, cold; Gui Jing: return lung, liver, the spleen channel; Effect: cooling blood for hemostasis, preventing phlegm from forming and stopping coughing, growing and blacking hair; Curing mainly: for spitting blood, bleeding from five sense organs or subcutaneous tissue, spitting of blood, has blood in stool, metrostaxis, cough due to lung-heat, blood-head alopecia, early whitening of beard and hair.

Pseudo-ginseng in Radix Notoginseng extract, nature and flavor, hardship sweet, micro-, temperature. Gui Jing, returns liver, stomach warp. Effect, loose stasis of blood hemostasis, subduing swelling and relieving pain. Curing mainly, for spitting of blood, spit blood, bleeding from five sense organs or subcutaneous tissue, has blood in stool, uterine bleeding, traumatic hemorrhage, chest and abdomen shouting pain, tumbling and swelling.

Flos Celosiae Cristatae in Flos Celosiae Cristatae extract, sweet, cool, nontoxic. Enter kidney channel, cool blood, hemostasis. Controlling blood under anal fistula, red white lower dysentery, spit blood, hemoptysis, blood drenches, women's metrorrhagia, leucorrhea with red and white discharge.

Cattail pollen in Pollen Tyjphae extract, taste is sweet, micro-pungent; Property is put down, and has blood-cooling hemostatic; Effect of blood circulation and stasis-eliminating medicine, cures mainly haematemesis; Spitting of blood; Bleeding from five sense organs or subcutaneous tissue; Standby dysentery; Have blood in stool; Uterine bleeding; Traumatic hemorrhage; Trusted subordinate's pain; Through closing stomachache; Postpartum the stasis of blood pain; Dysmenorrhoea; Tumbling and swelling; Blood strangury and dry pain; Under band; Double tongue; Aphtha; Ear; Uncontrolled urination is itched.

Crinis Carbonisatus in Crinis Carbonisatus extract, nature and flavor are bitter, flat. Gui Jing returns liver, stomach warp. Effect astringing to arrest bleeding, stagnation resolvation, diuresis. Curing mainly for spitting blood, spitting of blood, bleeding from five sense organs or subcutaneous tissue, blood drenches, and hematuria, has blood in stool, uterine bleeding, traumatic hemorrhage, dysuria.

Herba Clinopodii in Herba Clinopodii extract is micro-hardship, puckery, cool.Return Liver Channel. Effect: astringing to arrest bleeding. For uterine bleeding, hematuria, nosebleed epistaxis, gingival hemorrhage, wound hemorrhage.

The Herba Artemisiae Anomalae of Herba Artemisiae Anomalae extract, meridian distribution of property and flavor: bitter. Temperature, the thoughts of returning home, liver, the spleen channel. Broken blood stimulates the menstrual flow, healing up sore and subduing swelling. Control through closing abdominal mass, chest abdominal distention and pain, postpartum the blood stasis of blood, wound, metal-inflicted wound is hemorrhage, and carbuncle poison is swollen.

Herba Agrimoniae in Herba Agrimoniae extract, bitter, puckery, tepor, flat. The thoughts of returning home, Liver Channel. Having and hold back hemostasis, only dysentery, kills effect of worm. Extensively for various hemorrhage card, such as: haematemesiss, hematuria, have blood in stool, uterine bleeding, spitting of blood, bleeding from five sense organs or subcutaneous tissue, dysentery, internal lesion caused by overexertion take off power, and carbuncle is swollen, falls dozen, the diseases such as wound hemorrhage.

Folium Callicarpae Formosanae in Folium Callicarpae Formosanae extract is pungent, bitter, flat. Return liver, spleen, stomach warp. Function: loose stasis of blood hemostasis, swelling and pain relieving. Leaf: for spitting blood, spitting of blood, bleeding from five sense organs or subcutaneous tissue, has blood in stool; External curing traumatic hemorrhage. Root: treating swelling and pain by traumatic injury, treating rheumatic ostealgia.

Above Chinese medicine all has the effect of hemostasis, single use can also, after compatibility, result of use is better. Hemostatic of traditional herb medicine extract is carried out suitable compatibility by the present invention; the existing Chinese medicine that local vascular is shunk and stop blooding, such as pseudo-ginseng, Folium Callicarpae Formosanae, field thistle, also acts on coagulation process, shortens the clotting time; increase platelet count and short solidifying Chinese medicine, such as Herba Agrimoniae, Folium Callicarpae Formosanae; Also having and strengthen Platelet factorⅢ activity, the shortening thrombokinase generation time, such as the bletilla striata; Having and increase zymoplasm in blood, such as pseudo-ginseng, cattail pollen, more have and can improve vessel wall function, strengthen capillary vessel to the resistibility of damage, the Chinese medicine reducing vascular permeability is such as sophora flower. Suppress the activity of Tryptase (plasmin): as white and, setose thistle, field thistle, garden burnet, Herba Agrimoniae. The one side that the existing promotion blood such as pseudo-ginseng, madder, cattail pollen are solidifying, also has and impels clot dissolution effect, and its function has hemostasis and promoting blood circulation to remove blood stasis function concurrently, is conducive to hemostasis and does not stay the stasis of blood, is specially adapted to hemostatic material. To sum up, various Chinese medicine is learnt from other's strong points to offset one's weaknesses, and has played maximum effect, has hemostasis, anti-inflammation, antibacterial, analgesia, cool blood, dissipating blood stasis for subsidence of swelling, convergence, activation blood coagulation system, promotes the effect of wound healing.

Compared with prior art, useful effect is in the present invention:

(1) micropore starch of the present invention has the effect of molecular sieve, can moisture in fast absorption blood, formed " instant gel ", mechanicalness shutoff blood vessel cut; Hyaluronic acid can immediately in absorbing blood moisture form hydrogel, thus blood is oozed in shutoff; Lasiosphaera fenzlii spore powder stores a large amount of micro-multiporous fiber, can the moisture in absorbing blood rapidly, mechanicalness shutoff blood vessel cut and capillary vessel can ooze blood, micropore starch, hyaluronic acid, this three of Lasiosphaera fenzlii spore powder form physical barriers jointly, wound can be isolated, play anti and the effect of instant hemostasis. Hemostatic of traditional herb medicine extract then has hemostasis, anti-inflammation, antibacterial, analgesia, cool blood, dissipating blood stasis for subsidence of swelling, convergence, activation blood coagulation system, promotes the effect of wound healing. The present invention's four kinds of materials organically combine, and significantly strengthen haemostatic effect, and anthemorrhagic speed significantly improves, and oozes the hemostasis in blood, the hemorrhage and hemorrhage district of thin vessels for various wound and the flesh tissue surface of a wound, Film with Preventing Adhesion, the hemostasis being specially adapted in performing the operation.

(2) in the multiporous fiber art of the present invention, compound hemostatic material biocompatibility meets GB/T16886.11-2011 requirement, and in multiporous fiber art, compound hemostatic material microbiological indicator meets GB15979-2002 requirement.

(3) the present invention can stop blooding rapidly, and the gel viscosity of formation is higher, can clog damaged tissue and blood vessel, and the present invention is easily swelling and cleaning in water, and residue greatly reduces; The present invention is stable and not easily decomposes, long preservative period, has storage advantage.

Embodiment

Below in conjunction with embodiment, the present invention is further illustrated.

Embodiment 1.

Compound hemostatic material in a kind of multiporous fiber art, is made up of the raw material of following weight percent:

Micropore starch 20%

Hyaluronic acid 40%

Lasiosphaera fenzlii spore powder 20%

Hemostatic of traditional herb medicine extract 20%.

The surface micropore diameter of described micropore starch is 6-15 μm, and the porosity of described micropore starch is more than 90%.

The pharmaceutical grade powder of described hyaluronic acid to be molecular-weight average be 500 dalton.

Described Lasiosphaera fenzlii spore powder is that Lasiosphaera fenzlii is peelled off outer foreskin, digs out content and sifts out spore, by spore 110 degrees Celsius of dryings, crosses the Lasiosphaera fenzlii spore powder that 100 orders obtain with upper screen cloth after pulverizing.

Described Hemostatic of traditional herb medicine extract is 10 parts of Herba seu Radix Cirsii Japonicis and the mixture of 20 parts of Herba Cirsii extract compositions.

Above-mentioned micropore starch, hyaluronic acid, Lasiosphaera fenzlii spore powder and Hemostatic of traditional herb medicine extract are mixed to get compound hemostatic material in a kind of multiporous fiber art.

Embodiment 2.

Micropore starch 40%

Hyaluronic acid 20%

Lasiosphaera fenzlii spore powder 25%

Hemostatic of traditional herb medicine extract 15%.

The pharmaceutical grade powder of described hyaluronic acid to be molecular-weight average be 1000 dalton.

Described Lasiosphaera fenzlii spore powder is that Lasiosphaera fenzlii is peelled off outer foreskin, digs out content and sifts out spore, by spore 120 degrees Celsius of dryings, crosses the Lasiosphaera fenzlii spore powder that 100 orders obtain with upper screen cloth after pulverizing.

The mixture that described Hemostatic of traditional herb medicine extract is made up of 20 parts of Radix Sangusorbae extracts, 40 parts of Ramie Root extracts, 30 parts of Flos Sophorae extracts, 30 parts of Pollen Tyjphae extracts, 25 parts of Herba Agrimoniae extracts.

Micropore starch, hyaluronic acid, Lasiosphaera fenzlii spore powder and Hemostatic of traditional herb medicine extract are mixed to get compound hemostatic material in a kind of multiporous fiber art.

Micropore starch, hyaluronic acid, Lasiosphaera fenzlii spore powder and Hemostatic of traditional herb medicine extract continue ultrasonic activation 2 hours after mixing.

Embodiment 3.

Micropore starch 30%

Hyaluronic acid 25%

Lasiosphaera fenzlii spore powder 20%

Hemostatic of traditional herb medicine extract 25%.

The pharmaceutical grade powder of described hyaluronic acid to be molecular-weight average be 2000 dalton.

Described Lasiosphaera fenzlii spore powder is that Lasiosphaera fenzlii is peelled off outer foreskin, digs out content and sifts out spore, by spore 130 degrees Celsius of dryings, crosses the Lasiosphaera fenzlii spore powder that 100 orders obtain with upper screen cloth after pulverizing.

Described Hemostatic of traditional herb medicine extract is the mixture of 10 parts of Radix Sangusorbae extracts, 20 parts of Madder extracts, 20 parts of Rhizoma Imperatae extracts, 10 parts of Flos Celosiae Cristatae extracts, 20 parts of Herba Clinopodii extracts, 5 parts of Herba Artemisiae Anomalae extracts, 10 parts of Folium Callicarpae Formosanae extract compositions.

Micropore starch, hyaluronic acid, Lasiosphaera fenzlii spore powder and Hemostatic of traditional herb medicine extract continue ultrasonic activation 5 hours after mixing.

Embodiment 4.

Micropore starch 35%

Hyaluronic acid 25%

Lasiosphaera fenzlii spore powder 20%

Hemostatic of traditional herb medicine extract 20%.

The pharmaceutical grade powder of described hyaluronic acid to be molecular-weight average be 800 dalton.

Described Hemostatic of traditional herb medicine extract is made up of the raw material of following weight part:

Herba seu Radix Cirsii Japonici 10 parts

Herba Cirsii extract 40 parts

Radix Sangusorbae extract 20 parts

Ramie Root extract 30 parts

Madder extract 5 parts

Pseudobulbus Bletillae (Rhizoma Bletillae) extract 30 parts

Receptaculum nelumbinis extract 20 parts

Rhizoma nelumbinis extract 40 parts

Rhizoma Imperatae extract 20 parts

Flos Sophorae extract 30 parts

Cacumen Platycladi extract 10 parts

Radix Notoginseng extract 30 parts

Pollen Tyjphae extract 10 parts

Crinis Carbonisatus extract 10 parts

Herba Clinopodii extract 5 parts.

Micropore starch, hyaluronic acid, Lasiosphaera fenzlii spore powder and Hemostatic of traditional herb medicine extract continue ultrasonic activation 3 hours after mixing.

Embodiment 5.

Micropore starch 40%

Hyaluronic acid 20%

Lasiosphaera fenzlii spore powder 30%

Hemostatic of traditional herb medicine extract 10%.

The pharmaceutical grade powder of described hyaluronic acid to be molecular-weight average be 1200 dalton.

Described Hemostatic of traditional herb medicine extract comprises the raw material of following weight part:

Herba seu Radix Cirsii Japonici 30 parts

Herba Cirsii extract 20 parts

Radix Sangusorbae extract 40 parts

Ramie Root extract 10 parts

Madder extract 10 parts

Pseudobulbus Bletillae (Rhizoma Bletillae) extract 10 parts

Receptaculum nelumbinis extract 40 parts

Rhizoma nelumbinis extract 20 parts

Rhizoma Imperatae extract 40 parts

Flos Sophorae extract 10 parts

Cacumen Platycladi extract 30 parts

Radix Notoginseng extract 10 parts

Pollen Tyjphae extract 30 parts

Crinis Carbonisatus extract 5 parts

Herba Clinopodii extract 10 parts.

Micropore starch, hyaluronic acid, Lasiosphaera fenzlii spore powder and Hemostatic of traditional herb medicine extract continue ultrasonic activation 4 hours after mixing.

Embodiment 6.

Micropore starch 35%

Hyaluronic acid 30%

Lasiosphaera fenzlii spore powder 20%

Hemostatic of traditional herb medicine extract 15%.

The pharmaceutical grade powder of described hyaluronic acid to be molecular-weight average be 1800 dalton.

Herba seu Radix Cirsii Japonici 30 parts

Herba Cirsii extract 20 parts

Radix Sangusorbae extract 20 parts

Ramie Root extract 30 parts

Madder extract 5 parts

Pseudobulbus Bletillae (Rhizoma Bletillae) extract 10 parts

Receptaculum nelumbinis extract 40 parts

Rhizoma nelumbinis extract 20 parts

Rhizoma Imperatae extract 20 parts

Flos Sophorae extract 30 parts

Cacumen Platycladi extract 10 parts

Radix Notoginseng extract 10 parts

Pollen Tyjphae extract 30 parts

Crinis Carbonisatus extract 5 parts

Herba Clinopodii extract 5 parts.

SIJIQING extract 30 parts

Lignum Dalbergiae Odoriferae extract 5 parts

Rhizoma Corydalis extract 20 parts

Flos Celosiae Cristatae extract 30 parts

Herba Artemisiae Anomalae extract 10 parts

Herba Agrimoniae extract 20 parts

Folium Callicarpae Formosanae extract 30 parts.

Embodiment 7.

Micropore starch 30%

Hyaluronic acid 20%

Lasiosphaera fenzlii spore powder 30%

Hemostatic of traditional herb medicine extract 20%.

Herba seu Radix Cirsii Japonici 20 parts

Herba Cirsii extract 30 parts

Radix Sangusorbae extract 30 parts

Ramie Root extract 20 parts

Madder extract 8 parts

Pseudobulbus Bletillae (Rhizoma Bletillae) extract 20 parts

Receptaculum nelumbinis extract 30 parts

Rhizoma nelumbinis extract 30 parts

Rhizoma Imperatae extract 30 parts

Flos Sophorae extract 20 parts

Cacumen Platycladi extract 20 parts

Radix Notoginseng extract 20 parts

Pollen Tyjphae extract 20 parts

Crinis Carbonisatus extract 8 parts

Herba Clinopodii extract 8 parts.

SIJIQING extract 20 parts

Lignum Dalbergiae Odoriferae extract 8 parts

Rhizoma Corydalis extract 25 parts

Flos Celosiae Cristatae extract 15 parts

Herba Artemisiae Anomalae extract 25 parts

Herba Agrimoniae extract 30 parts

Folium Callicarpae Formosanae extract 25 parts.

Embodiment 8.

Micropore starch 25%

Hyaluronic acid 35%

Lasiosphaera fenzlii spore powder 25%

Hemostatic of traditional herb medicine extract 15%.

The pharmaceutical grade powder of described hyaluronic acid to be molecular-weight average be 900 dalton.

Herba seu Radix Cirsii Japonici 10 parts

Herba Cirsii extract 40 parts

Radix Sangusorbae extract 40 parts

Ramie Root extract 10 parts

Madder extract 10 parts

Pseudobulbus Bletillae (Rhizoma Bletillae) extract 30 parts

Receptaculum nelumbinis extract 20 parts

Rhizoma nelumbinis extract 40 parts

Rhizoma Imperatae extract 40 parts

Flos Sophorae extract 10 parts

Cacumen Platycladi extract 30 parts

Radix Notoginseng extract 30 parts

Pollen Tyjphae extract 10 parts

Crinis Carbonisatus extract 10 parts

Herba Clinopodii extract 10 parts.

SIJIQING extract 10 parts

Lignum Dalbergiae Odoriferae extract 10 parts

Rhizoma Corydalis extract 40 parts

Flos Celosiae Cristatae extract 10 parts

Herba Artemisiae Anomalae extract 30 parts

Herba Agrimoniae extract 40 parts

Folium Callicarpae Formosanae extract 10 parts.

Hemostasis usefulness measures and compares:

Laboratory animal and grouping are grown up healthy rabbits 50 (offer of Guangxi Medical University's experimentation on animals center), and body weight 2.5-3kg, male and female are not limit, experimental group and control group, often organize each 25, in each pressing time section each 5. Every rabbit both sides kidney makes long 1cm, and the dark hemorrhage surface of a wound of 0.5cm size, stops blooding to the hemorrhage surface of a wound with the hemostatic material of embodiment of the present invention 1-8 at random, taking Arista styptic powder to the hemostasis of another side surface of a wound as comparison. Experimental group is the application of embodiment of the present invention 1-8 hemostatic material, and control group is the application of Arista styptic powder.

Experimental technique and observation index

Hemorrhage Modelling: 3% pentobarbital sodium inj (30-40mg/kg) is anaesthetized along family's rabbit ear along intravenous injection, experiment table has been fixed rabbit, aseptic technique opens abdomen by layer, expose rabbit kidney, two longitudinal incisioies are made at kidney different positions at random with knife blade, long about 1cm, dark about 0.5cm, cutting part blood was with after claiming the dry gauze of weight to blot, with the hemostatic material of 1g embodiment of the present invention 1-8, the hemorrhage surface of a wound is stopped blooding at random, taking 1gArista powder to the hemostasis of another side surface of a wound as comparison, use the gauze pressing surface of a wound, oppress 10s respectively, 20s, 30s, 40s, 50s also records amount of bleeding and the bleeding stopping period of each time group.

Hemorrhage index observing

The bleeding stopping period kidney hemorrhagic disease surface of a wound is observed: calculates and is applied to the surface of a wound to surface of a wound blood from the present invention or Arista styptic powder and oozes out stopping or the red dye area of styptic powder particle no longer expands and is recorded as bleeding stopping period.

Amount of bleeding analytical balance accurate weighing experimental group and the front dry gauze weight rear with hemostasis of control group hemostasis in advance, calculates amount of bleeding (the front weight/blood proportion of the rear weight-hemostasis of amount of bleeding=hemostasis) respectively.

Result: general proterties and ultrastructure: the hemostatic material of the present invention is white powder particle, visual inspection, granular size equal one, relatively Arista styptic powder is fine and smooth for grain pattern, under scanning electron microscope, starch granules diameter is about 20 μm, and there is multiple pore passage structure inside, aperture is about 1.5 μm, and pore size is substantially identical. Arista styptic powder is white powder particle, and under scanning electron microscope, visible particle surface is made up of multiple crack, and without pore passage structure, particle volume is about 1-2 times of the present invention.

Experimental result shows, and when oppressing 10s, 20s, kidney hemorrhagic disease amount is big, and the bleeding time is long. Pressing time is more than, after 30s, amount of bleeding and bleeding time change are obvious, and both obviously shorten, and after compressing 40s, 50s, hemorrhage index rangeability reduces further. Experimental data shows: experimental group and control group all need compressing more than 30s, and haemostatic effect could embody. Table 1 is in different pressing time, the hemostatic material of embodiment of the present invention 1-8 and the comparison of Arista styptic powder amount of bleeding and bleeding stopping period.

Table 1

Can find out that haemostatic effect of the present invention is obviously better than Arista styptic powder by table 1.

Haemostatic effect gross examination of skeletal muscle

After exposing kidney, after otch is performed an operation on normal kidney surface, blood is gushed out rapidly, after blotting blood with dry gauze, spills the styptic powder of 1g the present invention fast, and hemorrhage district starch turns red immediately by white, and dry gauze continues compressing. 20s compressing group, removes gauze, and the hemorrhage surface of a wound has a small amount of blood to ooze out, and the red dye area of styptic powder of the present invention slowly expands;30s compressing group, the hemorrhage basic stopping of kidney otch, without obvious oozing phenomenon; 40s and 50s compressing group, after removing gauze, it is seen that the styptic powder of the present invention attaches to cut surface in the pasty state, stopped bleeding.

Finally should be noted that; above embodiment is only in order to illustrate the technical scheme of the present invention; but not limiting the scope of the invention; although having done to explain to the present invention with reference to better embodiment; it will be understood by those within the art that; the technical scheme of the present invention can be modified or equivalent replacement, and not depart from essence and the scope of technical solution of the present invention.

Claims

1. compound hemostatic material in a multiporous fiber art, it is characterised in that: in this multiporous fiber art, compound hemostatic material is made up of the raw material of following weight percent:

Micropore starch 20-40%

Hyaluronic acid 20-40%

Lasiosphaera fenzlii spore powder 20-40%

Hemostatic of traditional herb medicine extract 10-30%.

2. compound hemostatic material in a kind of multiporous fiber art according to claim 1, it is characterised in that: in this multiporous fiber art, compound hemostatic material is made up of the raw material of following weight percent:

Micropore starch 20-35%

Hyaluronic acid 20-35%

Lasiosphaera fenzlii spore powder 20-35%

Hemostatic of traditional herb medicine extract 15-30%.

3. compound hemostatic material in a kind of multiporous fiber art according to claim 1, it is characterised in that: the surface micropore diameter of described micropore starch is 6-15 μm, and the porosity of described micropore starch is more than 90%.

4. compound hemostatic material in a kind of multiporous fiber art according to claim 1, it is characterised in that: the pharmaceutical grade powder of described hyaluronic acid to be molecular-weight average be 500-2000 dalton.

5. compound hemostatic material in a kind of multiporous fiber art according to claim 1, it is characterized in that: described Lasiosphaera fenzlii spore powder is that Lasiosphaera fenzlii is peelled off outer foreskin, dig out content and sift out spore, by spore 110-130 degree Celsius of drying, after pulverizing, cross the Lasiosphaera fenzlii spore powder that 100 orders obtain with upper screen cloth.

6. compound hemostatic material in a kind of multiporous fiber art according to claim 1, it is characterized in that: described Hemostatic of traditional herb medicine extract is Herba seu Radix Cirsii Japonici, Herba Cirsii extract, SIJIQING extract, Radix Sangusorbae extract, Ramie Root extract, Madder extract, Lignum Dalbergiae Odoriferae extract, Pseudobulbus Bletillae (Rhizoma Bletillae) extract, Receptaculum nelumbinis extract, rhizoma nelumbinis extract, Rhizoma Corydalis extract, Rhizoma Imperatae extract, Flos Sophorae extract, Cacumen Platycladi extract, Radix Notoginseng extract, Flos Celosiae Cristatae extract, Pollen Tyjphae extract, Crinis Carbonisatus extract, Herba Clinopodii extract, Herba Artemisiae Anomalae extract, Herba Agrimoniae extract, one or more mixture of Folium Callicarpae Formosanae extract.

7. compound hemostatic material in a kind of multiporous fiber art according to claim 6, it is characterised in that: described Hemostatic of traditional herb medicine extract comprises the raw material of following weight part:

Herba seu Radix Cirsii Japonici 10-30 part

Herba Cirsii extract 20-40 part

Radix Sangusorbae extract 20-40 part

Ramie Root extract 10-30 part

Madder extract 5-10 part

Pseudobulbus Bletillae (Rhizoma Bletillae) extract 10-30 part

Receptaculum nelumbinis extract 20-40 part

Rhizoma nelumbinis extract 20-40 part

Rhizoma Imperatae extract 20-40 part

Flos Sophorae extract 10-30 part

Cacumen Platycladi extract 10-30 part

Radix Notoginseng extract 10-30 part

Pollen Tyjphae extract 10-30 part

Crinis Carbonisatus extract 5-10 part

Herba Clinopodii extract 5-10 part.

8. compound hemostatic material in a kind of multiporous fiber art according to claim 7, it is characterised in that: described Hemostatic of traditional herb medicine extract also comprises the raw material of following weight part:

SIJIQING extract 10-30 part

Lignum Dalbergiae Odoriferae extract 5-10 part

Rhizoma Corydalis extract 20-40 part

Flos Celosiae Cristatae extract 10-30 part

Herba Artemisiae Anomalae extract 10-30 part

Herba Agrimoniae extract 20-40 part

Folium Callicarpae Formosanae extract 10-30 part.

9. the preparation method of compound hemostatic material in a kind of multiporous fiber art according to claim 1, it is characterised in that: micropore starch, hyaluronic acid, Lasiosphaera fenzlii spore powder and Hemostatic of traditional herb medicine extract are mixed to get compound hemostatic material in a kind of multiporous fiber art.

10. the preparation method of compound hemostatic material in a kind of multiporous fiber art according to claim 9, it is characterised in that: micropore starch, hyaluronic acid, Lasiosphaera fenzlii spore powder and Hemostatic of traditional herb medicine extract continue ultrasonic activation 1-5 hour after mixing.