CN105669847A - 大豆蛋白GmVPS9a1在调控植物贮藏蛋白分选中的应用 - Google Patents
大豆蛋白GmVPS9a1在调控植物贮藏蛋白分选中的应用 Download PDFInfo
- Publication number
- CN105669847A CN105669847A CN201510941174.2A CN201510941174A CN105669847A CN 105669847 A CN105669847 A CN 105669847A CN 201510941174 A CN201510941174 A CN 201510941174A CN 105669847 A CN105669847 A CN 105669847A
- Authority
- CN
- China
- Prior art keywords
- protein
- gmvps9a1
- plant
- sorting
- storage protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000031787 nutrient reservoir activity Effects 0.000 title claims abstract description 64
- 230000033228 biological regulation Effects 0.000 title claims abstract description 17
- 108010073771 Soybean Proteins Proteins 0.000 title abstract description 6
- 229940001941 soy protein Drugs 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 102
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 49
- 230000009261 transgenic effect Effects 0.000 claims abstract description 34
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 5
- 241000196324 Embryophyta Species 0.000 claims description 125
- 235000018102 proteins Nutrition 0.000 claims description 44
- 230000014509 gene expression Effects 0.000 claims description 32
- 108020004414 DNA Proteins 0.000 claims description 25
- 239000002773 nucleotide Substances 0.000 claims description 24
- 125000003729 nucleotide group Chemical group 0.000 claims description 24
- 239000013598 vector Substances 0.000 claims description 23
- 108020004707 nucleic acids Proteins 0.000 claims description 22
- 102000039446 nucleic acids Human genes 0.000 claims description 22
- 150000007523 nucleic acids Chemical class 0.000 claims description 22
- 239000012620 biological material Substances 0.000 claims description 18
- 150000001413 amino acids Chemical class 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 13
- 235000001014 amino acid Nutrition 0.000 claims description 10
- 239000002299 complementary DNA Substances 0.000 claims description 9
- 244000005700 microbiome Species 0.000 claims description 9
- 210000000056 organ Anatomy 0.000 claims description 7
- 241000209510 Liliopsida Species 0.000 claims description 6
- 241001233957 eudicotyledons Species 0.000 claims description 6
- 102000053602 DNA Human genes 0.000 claims description 4
- 230000002159 abnormal effect Effects 0.000 claims description 4
- 125000000539 amino acid group Chemical group 0.000 claims description 3
- 238000009396 hybridization Methods 0.000 claims description 3
- 101710132614 Protein B4 Proteins 0.000 claims description 2
- 230000008034 disappearance Effects 0.000 claims description 2
- 238000009395 breeding Methods 0.000 abstract description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract 1
- 210000004899 c-terminal region Anatomy 0.000 abstract 1
- 240000007594 Oryza sativa Species 0.000 description 21
- 235000007164 Oryza sativa Nutrition 0.000 description 21
- 235000009566 rice Nutrition 0.000 description 17
- 238000012216 screening Methods 0.000 description 11
- 241000589158 Agrobacterium Species 0.000 description 10
- 108010068370 Glutens Proteins 0.000 description 10
- 230000009182 swimming Effects 0.000 description 10
- 239000001963 growth medium Substances 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 8
- 239000007787 solid Substances 0.000 description 8
- 239000002243 precursor Substances 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 6
- 102000014303 Amyloid beta-Protein Precursor Human genes 0.000 description 5
- 108010079054 Amyloid beta-Protein Precursor Proteins 0.000 description 5
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 5
- 206010020649 Hyperkeratosis Diseases 0.000 description 5
- 239000013604 expression vector Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 244000068988 Glycine max Species 0.000 description 4
- 235000010469 Glycine max Nutrition 0.000 description 4
- 230000002378 acidificating effect Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 239000003623 enhancer Substances 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000013642 negative control Substances 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 210000000582 semen Anatomy 0.000 description 4
- 235000019710 soybean protein Nutrition 0.000 description 4
- 238000013518 transcription Methods 0.000 description 4
- 230000035897 transcription Effects 0.000 description 4
- 108020004705 Codon Proteins 0.000 description 3
- 108010016634 Seed Storage Proteins Proteins 0.000 description 3
- 108091081024 Start codon Proteins 0.000 description 3
- 239000002585 base Substances 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 230000000050 nutritive effect Effects 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000013519 translation Methods 0.000 description 3
- 241000701489 Cauliflower mosaic virus Species 0.000 description 2
- LMKYZBGVKHTLTN-NKWVEPMBSA-N D-nopaline Chemical compound NC(=N)NCCC[C@@H](C(O)=O)N[C@@H](C(O)=O)CCC(O)=O LMKYZBGVKHTLTN-NKWVEPMBSA-N 0.000 description 2
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 2
- 240000008467 Oryza sativa Japonica Group Species 0.000 description 2
- 108010028230 Trp-Ser- His-Pro-Gln-Phe-Glu-Lys Proteins 0.000 description 2
- 108091023045 Untranslated Region Proteins 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000003501 co-culture Methods 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 239000004009 herbicide Substances 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 230000008488 polyadenylation Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 210000003934 vacuole Anatomy 0.000 description 2
- GEWDNTWNSAZUDX-WQMVXFAESA-N (-)-methyl jasmonate Chemical compound CC\C=C/C[C@@H]1[C@@H](CC(=O)OC)CCC1=O GEWDNTWNSAZUDX-WQMVXFAESA-N 0.000 description 1
- LWTDZKXXJRRKDG-KXBFYZLASA-N (-)-phaseollin Chemical compound C1OC2=CC(O)=CC=C2[C@H]2[C@@H]1C1=CC=C3OC(C)(C)C=CC3=C1O2 LWTDZKXXJRRKDG-KXBFYZLASA-N 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 101710102211 11S globulin Proteins 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 101001033883 Cenchritis muricatus Protease inhibitor 2 Proteins 0.000 description 1
- 208000003322 Coinfection Diseases 0.000 description 1
- 101710190853 Cruciferin Proteins 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 1
- 101150074155 DHFR gene Proteins 0.000 description 1
- 101150111720 EPSPS gene Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- XZWYTXMRWQJBGX-VXBMVYAYSA-N FLAG peptide Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@@H](N)CC(O)=O)CC1=CC=C(O)C=C1 XZWYTXMRWQJBGX-VXBMVYAYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000005562 Glyphosate Substances 0.000 description 1
- 108010093488 His-His-His-His-His-His Proteins 0.000 description 1
- 101100288095 Klebsiella pneumoniae neo gene Proteins 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
- 102000002704 Leucyl aminopeptidase Human genes 0.000 description 1
- 108010004098 Leucyl aminopeptidase Proteins 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 241000710118 Maize chlorotic mottle virus Species 0.000 description 1
- 108091022912 Mannose-6-Phosphate Isomerase Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000014171 Milk Proteins Human genes 0.000 description 1
- 108010011756 Milk Proteins Proteins 0.000 description 1
- 101710135898 Myc proto-oncogene protein Proteins 0.000 description 1
- 102100038895 Myc proto-oncogene protein Human genes 0.000 description 1
- 101710202365 Napin Proteins 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 101710089395 Oleosin Proteins 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N Phosphinothricin Natural products CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- 108091034057 RNA (poly(A)) Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 125000000066 S-methyl group Chemical group [H]C([H])([H])S* 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000592344 Spermatophyta Species 0.000 description 1
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 1
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 101710150448 Transcriptional regulator Myc Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 101150103518 bar gene Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229940027138 cambia Drugs 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- KXZOIWWTXOCYKR-UHFFFAOYSA-M diclofenac potassium Chemical compound [K+].[O-]C(=O)CC1=CC=CC=C1NC1=C(Cl)C=CC=C1Cl KXZOIWWTXOCYKR-UHFFFAOYSA-M 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 1
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 1
- 229940097068 glyphosate Drugs 0.000 description 1
- 101150054900 gus gene Proteins 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 101150029559 hph gene Proteins 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 229960000485 methotrexate Drugs 0.000 description 1
- GEWDNTWNSAZUDX-UHFFFAOYSA-N methyl 7-epi-jasmonate Natural products CCC=CCC1C(CC(=O)OC)CCC1=O GEWDNTWNSAZUDX-UHFFFAOYSA-N 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000021239 milk protein Nutrition 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- LWTDZKXXJRRKDG-UHFFFAOYSA-N phaseollin Natural products C1OC2=CC(O)=CC=C2C2C1C1=CC=C3OC(C)(C)C=CC3=C1O2 LWTDZKXXJRRKDG-UHFFFAOYSA-N 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229960004889 salicylic acid Drugs 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000012256 transgenic experiment Methods 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 108010073119 vacuolar processing enzyme Proteins 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Nutrition Science (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
标签 | 残基 | 序列 |
Poly-Arg | 5-6(通常为5个) | RRRRR |
Poly-His | 2-10(通常为6个) | HHHHHH |
FLAG | 8 | DYKDDDDK |
Strep-tag II | 8 | WSHPQFEK |
c-myc | 10 | EQKLISEEDL |
Claims (10)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510437013 | 2015-07-22 | ||
CN201510437013X | 2015-07-22 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105669847A true CN105669847A (zh) | 2016-06-15 |
CN105669847B CN105669847B (zh) | 2019-05-07 |
Family
ID=56189562
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510941174.2A Active CN105669847B (zh) | 2015-07-22 | 2015-12-16 | 大豆蛋白GmVPS9a1在调控植物贮藏蛋白分选中的应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105669847B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107022016A (zh) * | 2017-05-15 | 2017-08-08 | 中国农业科学院作物科学研究所 | 一种调控植物贮藏蛋白分选的蛋白质GmGPA3及其编码基因与应用 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101960013A (zh) * | 2008-01-30 | 2011-01-26 | 先正达参股股份有限公司 | 在植物组织中异源多肽的高水平表达和积累 |
-
2015
- 2015-12-16 CN CN201510941174.2A patent/CN105669847B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101960013A (zh) * | 2008-01-30 | 2011-01-26 | 先正达参股股份有限公司 | 在植物组织中异源多肽的高水平表达和积累 |
Non-Patent Citations (2)
Title |
---|
ACCESSION:XM_003526606.1: "PREDICTED: vacuolar protein sorting-associated protein 9A-like [Glycine max]", 《GENBANK》 * |
YULONG REN ET AL.: "GLUTELIN PRECURSOR ACCUMULATION3 Encodes a Regulator of Post-Golgi Vesicular Traffic Essential for Vacuolar Protein Sorting in Rice Endosperm", 《THE PLANT CELL》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107022016A (zh) * | 2017-05-15 | 2017-08-08 | 中国农业科学院作物科学研究所 | 一种调控植物贮藏蛋白分选的蛋白质GmGPA3及其编码基因与应用 |
CN107022016B (zh) * | 2017-05-15 | 2020-01-14 | 中国农业科学院作物科学研究所 | 一种调控植物贮藏蛋白分选的蛋白质GmGPA3及其编码基因与应用 |
Also Published As
Publication number | Publication date |
---|---|
CN105669847B (zh) | 2019-05-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA2366104C (en) | Ultra-fast transformation technique for monocotyledons | |
CN105037521B (zh) | 一种与植物抗逆性相关蛋白TaWrky48及其编码基因与应用 | |
CN109721648B (zh) | 一种水稻株型相关蛋白及其编码基因和应用 | |
CN106188257B (zh) | 大豆转录因子GmbZIP336及其编码基因在调控种子粒重中的应用 | |
CN105859860A (zh) | 抗病相关蛋白在调控植物抗病性中的应用 | |
AU759570B2 (en) | Transgenic lemnaceae | |
CN108070578A (zh) | 一种与植物耐逆性相关蛋白GmHAD1及其编码基因与应用 | |
US9803212B2 (en) | Regulation of translation of heterologously expressed genes | |
CN105061569B (zh) | 一种与植物抗逆性相关的SiMYB107蛋白及其相关生物材料与应用 | |
CN105713079B (zh) | 蛋白质及其相关生物材料在提高植物产量中的应用 | |
CN104744579B (zh) | 抗逆相关蛋白GmL16在调控植物抗逆性中的应用 | |
CN105820220B (zh) | 抗逆相关蛋白及其编码基因在调控植物耐碱性中的应用 | |
Streatfield et al. | Identification of maize embryo-preferred promoters suitable for high-level heterologous protein production | |
CN105985417B (zh) | 生长相关蛋白grp2在调控植物生长中的应用 | |
CN106279386B (zh) | 一种水稻穗顶部生长发育相关蛋白及其编码基因与应用 | |
CN105504031B (zh) | 来源于大豆的粒重相关蛋白及其相关生物材料与应用 | |
CN106047833A (zh) | OsCIPK31及其编码基因在调控植物除草剂抗性中的应用 | |
CN105669847A (zh) | 大豆蛋白GmVPS9a1在调控植物贮藏蛋白分选中的应用 | |
CN105693834B (zh) | 大豆蛋白GmVPS9a2在调控植物贮藏蛋白分选中的应用 | |
CN105985419B (zh) | 生长相关蛋白grp1在调控植物生长中的应用 | |
CN105985418B (zh) | 生长相关蛋白grp4在调控植物生长中的应用 | |
CN106674339A (zh) | 蛋白质在调控植物抗逆性中的应用 | |
CN114164212B (zh) | 玉米营养器官特异性表达启动子及其应用 | |
CN107022016B (zh) | 一种调控植物贮藏蛋白分选的蛋白质GmGPA3及其编码基因与应用 | |
CN105985420B (zh) | 生长相关蛋白grp3在调控植物生长中的应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240205 Address after: 102200, 7th floor, Building 4, No. 4 Life Park Road, Zhongguancun Life Science Park, Changping District, Beijing Patentee after: BEIJING COMPASS BIOTECHNOLOGY TECHNOLOGY Co.,Ltd. Country or region after: China Address before: 100081 No. 12 South Main Street, Haidian District, Beijing, Zhongguancun Patentee before: INSTITUTE OF CROP SCIENCES, CHINESE ACADEMY OF AGRICULTURAL SCIENCES Country or region before: China |