CN105661018A - Method for adding bacillus thuringiensis H1 to chicken feed to prevent breeding of fly maggots in chicken manure - Google Patents

Method for adding bacillus thuringiensis H1 to chicken feed to prevent breeding of fly maggots in chicken manure Download PDF

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Publication number
CN105661018A
CN105661018A CN201610217458.1A CN201610217458A CN105661018A CN 105661018 A CN105661018 A CN 105661018A CN 201610217458 A CN201610217458 A CN 201610217458A CN 105661018 A CN105661018 A CN 105661018A
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China
Prior art keywords
chicken
bacillus thuringiensis
feed
spore
feeding
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Pending
Application number
CN201610217458.1A
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Chinese (zh)
Inventor
张忠
张瑞玲
陈丹
刘婧
庄桂芬
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Taishan Medical University
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Taishan Medical University
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Publication date
Application filed by Taishan Medical University filed Critical Taishan Medical University
Priority to CN201610217458.1A priority Critical patent/CN105661018A/en
Publication of CN105661018A publication Critical patent/CN105661018A/en
Pending legal-status Critical Current

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Abstract

The invention discloses a method for adding bacillus thuringiensis H1 to chicken feed to prevent breeding of fly maggots in chicken manure.The method comprises the steps of 1, preparing spore powder of bacillus thuringiensis H1, wherein the spore content is 10<8>/mg; 2, mixing the powder prepared in the step 1 with chicken feed to make powder content 1 mg/kg; 3, feeding chicken with the feed obtained from the step 2 once every three days, and feeding the chicken with normal feed which does not contain bacillus thuringiensis spore at other times.Compared with the prior art, the method has the advantages that by adding bacillus thuringiensis to chicken feed to control breeding of fly maggots in chicken manure, safety is improved compared with traditional chemical control methods, environment pollution is avoided, no harm is caused to human body, and the chicken house fly control method is environmentally friendly and capable of saving time and labor.

Description

Chicken feed is added Bacillus thuringiensis H1 and prevents and treats the method that in chicken manure, fly larvae is multiplied
Technical field
The present invention relates to biotechnology, particularly relate to a kind of Bacillus thuringiensis H1 that adds in chicken feed and prevent and treat the method that in chicken manure, fly larvae is multiplied.
Background technology
Flies is important Vector factors, can the multiple disease such as mechanical transmission dysentery, typhoid fever, cholera, tuberculosis, trachoma, poliomyelitis, hepatitis and parasitic disease or pollute human foods, threaten human health, the life of interference people. Flies is multiplied a lot in the chicken manure of chicken farm, prevents and treats to raise the very difficult chemical agent of safety. In feed lot, Biological control flies is a kind of method preferably. Bacillus thuringiensis is a kind of eco-friendly insecticide, human body, Wildlife, insect pollinator and other most beneficial insects are almost harmless, the fly larvae of housefly is had good control action by its H1 bacterial strain, but utilize the method that fly larvae is substantially manually or machine sprays in H1 bacterial strain preventing and treating chicken manure to carry out, waste time and energy, and some corner is then difficult to process, effect is not notable. Accordingly, it would be desirable to explore the method that in plant, flies is carried out " innoxious control ".
Summary of the invention
The present invention is to solve above-mentioned deficiency, it is provided that a kind of Bacillus thuringiensis H1 that adds in chicken feed prevents and treats the method that in chicken manure, fly larvae is multiplied. The present invention namely by chicken feed in 1mg/kg ratio add spore content be 108The bacillus thuringiensis H1 of individual/mg, chicken eats after the spore of bacillus thuringiensis, the growth of chicken is had no effect, and in chicken manure, still suffer from the spore of substantial amounts of bacillus thuringiensis, when flies is laid eggs on chicken manure, hatching and the growth of flies ovum all can be produced significant inhibitory action by the spore of residual, thus reaching to eliminate the purpose of flies, the method saves time, laborsaving, and the chicken raised is nontoxic, harmless, environmentally safe, is a kind of good method multiplied in chicken house control flies.
The above-mentioned purpose of the present invention is realized by following technical scheme: a kind of Bacillus thuringiensis H1 that adds in chicken feed prevents and treats the method that in chicken manure, fly larvae is multiplied, it is characterised in that: comprise the following steps:
(1) preparing the spore powder of bacillus thuringiensis H1, making spore content is 108Individual/mg;
(2) powder of preparation in step (1) is mixed in chicken feed so that it is content reaches 1mg/kg;
(3) chicken that the feedstuff feeding in step (2) is raised, every 3d feeding 1 time, all the other time feeding chow diet without bacillus thuringiensis spore.
Present invention advantage compared with prior art is: the present invention adds the method that bacillus thuringiensis controls to multiply in the chicken manure of flies in chicken feed, with traditional chemical prevent and treat compared with safer, environmentally safe, harmless, it is a kind of environmental protection, time saving and energy saving chicken house flies control method.
Accompanying drawing explanation
Fig. 1 is Eggs of Musca Domestica Vicina percentage of pupation chart in different number of days chicken manure after feeding is containing bacillus thuringiensis feedstuff.
Fig. 2 is Eggs of Musca Domestica Vicina eclosion rate chart in different number of days chicken manure after feeding is containing bacillus thuringiensis feedstuff.
Fig. 3 is the prevention effect chart that housefly is pupated and sprouts wings by the feeding chicken manure containing bacillus thuringiensis chicken feed.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail.
A kind of Bacillus thuringiensis H1 that adds in chicken feed prevents and treats the method that in chicken manure, fly larvae is multiplied, it is characterised in that: comprise the following steps:
(1) preparing the spore powder of bacillus thuringiensis H1, making spore content is 108Individual/mg;
(2) powder of preparation in step (1) is mixed in chicken feed so that it is content reaches 1mg/kg;
(3) chicken that the feedstuff feeding in step (2) is raised, every 3d feeding 1 time, all the other time feeding chow diet without bacillus thuringiensis spore.
Following instance is further illustrating the present invention.
Adding spore content in chicken feed is 108The bacillus thuringiensis of individual/mg, its content in feedstuff is made to reach 1mg/kg, after feeding chicken, every day collects chicken manure, is placed in the plastic casing being sized to 20 × 10 × 5cm, and every box is about 500g, move into primiparity Eggs of Musca Domestica Vicina 50, pupate number and the emerging adult number of record housefly, and calculate percentage of pupation and eclosion rate, often process and repeat 3 times. With the chicken manure of feeding chow diet for comparison. It is shown that feeding is containing in the chicken manure of 1-3d after bacillus thuringiensis feedstuff, the percentage of pupation of Musca domestica larva, compared with the chicken manure of 4-6d after comparison and feeding, is all remarkably decreased (as shown in Figure 1); Meanwhile, housefly eclosion rate in the chicken manure of 1-6d after feeding is containing bacillus thuringiensis feedstuff is all substantially less than comparison, and wherein feeding 2-5d chicken manure is better to the inhibition of the emergence of housefly, with the best results (as shown in Figure 2) of 2-3d. Chicken feed is added bacillus thuringiensis and can effectively suppress multiplying of housefly in chicken manure, it is suppressed that the pupating and sprout wings of housefly, wherein to feed inhibition the best (as shown in Figure 3) of rear 2-4d chicken manure.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every equivalent structure utilizing description of the present invention and embodiment content to make or equivalence flow process conversion; or directly or indirectly it is used in other relevant technical fields, all in like manner include in the scope of patent protection of the present invention.

Claims (1)

1. one kind is added the method that in Bacillus thuringiensis H1 preventing and treating chicken manure, fly larvae is multiplied in chicken feed, it is characterised in that: comprise the following steps:
(1) preparing the spore powder of bacillus thuringiensis H1, making spore content is 108Individual/mg;
(2) powder of preparation in step (1) is mixed in chicken feed so that it is content reaches 1mg/kg;
(3) chicken that the feedstuff feeding in step (2) is raised, every 3d feeding 1 time, all the other time feeding chow diet without bacillus thuringiensis spore.
CN201610217458.1A 2016-04-08 2016-04-08 Method for adding bacillus thuringiensis H1 to chicken feed to prevent breeding of fly maggots in chicken manure Pending CN105661018A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610217458.1A CN105661018A (en) 2016-04-08 2016-04-08 Method for adding bacillus thuringiensis H1 to chicken feed to prevent breeding of fly maggots in chicken manure

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610217458.1A CN105661018A (en) 2016-04-08 2016-04-08 Method for adding bacillus thuringiensis H1 to chicken feed to prevent breeding of fly maggots in chicken manure

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CN105661018A true CN105661018A (en) 2016-06-15

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101481668A (en) * 2009-01-15 2009-07-15 山东省科学院中日友好生物技术研究中心 Bacillus thuringiensis suspending agent for killing cotton spider mites, preparation and use
CN102006784A (en) * 2008-04-30 2011-04-06 株式会社美你康 Livestock feed, method for production thereof, and use thereof
CN103125778A (en) * 2013-03-20 2013-06-05 邹平泰康生物饲料有限公司 Poultry feed additive and preparation method thereof
US20140072546A1 (en) * 2013-11-14 2014-03-13 Valent U.S.A. Corporation Compositions and methods for enhancing insecticidal seed treatment efficacy

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102006784A (en) * 2008-04-30 2011-04-06 株式会社美你康 Livestock feed, method for production thereof, and use thereof
CN101481668A (en) * 2009-01-15 2009-07-15 山东省科学院中日友好生物技术研究中心 Bacillus thuringiensis suspending agent for killing cotton spider mites, preparation and use
CN103125778A (en) * 2013-03-20 2013-06-05 邹平泰康生物饲料有限公司 Poultry feed additive and preparation method thereof
US20140072546A1 (en) * 2013-11-14 2014-03-13 Valent U.S.A. Corporation Compositions and methods for enhancing insecticidal seed treatment efficacy

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
冷培恩 等: "蝇类防制工作进展与发展展望", 《中国媒介生物学及控制杂志》 *

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Application publication date: 20160615