CN105647790B - The application method of microorganism multi-parameter comprehensive test platform - Google Patents
The application method of microorganism multi-parameter comprehensive test platform Download PDFInfo
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- CN105647790B CN105647790B CN201610127169.2A CN201610127169A CN105647790B CN 105647790 B CN105647790 B CN 105647790B CN 201610127169 A CN201610127169 A CN 201610127169A CN 105647790 B CN105647790 B CN 105647790B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
- G01N27/22—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance
- G01N27/221—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance by investigating the dielectric properties
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
Abstract
The present invention discloses the application method of microorganism multi-parameter comprehensive test platform, the sample introduction of microorganism test solution is accurately controlled using high-purity plunger piston type micro sampling pump, so that in whole test process stabilised microorganism sample liquid inlet amount, so that microorganism detection test solution enters from liquid inlet, by microchannel, from liquid outlet outflow；Camera aperture is connected with micro- bloom spectrometer, and opens background light source, bias light is provided through backlight hole for micro- bloom spectrometer, to realize the collection to the micro- high spectrum image information of microorganism；Capacitive dielectric electrode, plane wave conduction pectinate electrodes and parallel pole connect dielectric spectra test equipment respectively, realize and the dielectric property and quantity of microorganism are detected；Optical fiber through hole is connected with fiber spectrometer, realizes and the spectral characteristic of microorganism is detected.The present invention is applied to the qualitative and quantitative detection of microorganism in liquid, realizes microorganism multiple information synchronous acquisition, improves detection efficiency and accuracy of detection.
Description
The present patent application is the divisional application of parent application " microorganism multi-parameter comprehensive test platform ", the Shen of parent application
Please number be 2015100275920, applying date of parent application is on January 20th, 2015.
Technical field
The invention belongs to microorganism detection field, especially a kind of microorganism multi-parameter comprehensive test platform.
Background technology
The direct measurement of harmful microorganisms is always the research of Microbiological detection of foods in microorganism detection especially food
Focus.At present, high light spectrum image-forming technology has been widely used for studying quality, characteristic (pH value and viscosity) and the pollutant of food
(microorganism and melamine).In addition, high light spectrum image-forming technology also can be directly used for detection spore or endosporous survival ability.
Dielectric is a kind of the material of polarization phenomenon, including gas, liquid and solid material can be produced under outside electric field action
Material.Dielectric constant is a dielectric important performance parameter.It is the Microscopic of dielectric internal structure, including electric charge
Distribution, chemical constitution, microscopic pattern etc..And dielectric cure sensor is just used for the dielectric constant for measuring dielectric material.Therefore build
Microorganism dielectric spectra and EO-1 hyperion synchro measure platform, are to realize the collection of microorganism multiple information to realize its direct quantitative
With effective trial of qualitative detection.Existing microorganism detection technology, is only the single characteristic or several to microorganism mostly
Individual characteristic carries out analysis detection, it is impossible to realize and the comprehensive analysis of many characteristics of microorganism is detected so that detection efficiency is relatively low.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is used on the basis of microflow control technique there is provided one kind
The microorganism multi-parameter comprehensive test platform of microorganism micro image collection, dielectric property and spectral characteristic synchronism detection.This hair
Bright system is simple in construction, design science is reasonable, can the efficient many characteristics realized to microbiological specimens, the complex synchronous of multi-parameter
Analysis detection.
The present invention solves its technical problem and is achieved through the following technical solutions：
A kind of microorganism multi-parameter comprehensive test platform, including, high-purity plunger piston type micro sampling pump, micro-fluidic chip,
Microlens, micro- bloom spectrometer, computer, fiber spectrometer, dielectric spectrometer.
The micro-fluidic chip, including：Microchannel, capacitive dielectric electrode, plane wave conduction pectinate electrodes, parallel electricity
Pole, optical fiber through hole, backlight hole and camera aperture.Micro-fluidic chip is on the whole sealing cavity structure, and it is internally provided with miniflow and led to
Road, liquid inlet and liquid outlet are respectively arranged with the two ends of microchannel；Direction along liquid inlet to liquid outlet is disposed with
Camera aperture, backlight hole, parallel pole, plane wave conduction pectinate electrodes and capacitive dielectric electrode；Camera aperture and the backlight hole
The front and back of micro-fluidic chip is symmetricly set on, the figure of the microorganism in runner can be gathered by camera aperture microscopic system
As information, by backlight hole, background light source can shine into microchannel, so as to provide background light source, the camera lens for microscopic system
Hole and backlight hole are sealed using quartz plate；The parallel pole includes upper parallel pole and lower parallel pole, and it is symmetrical arranged
In the both sides of micro-fluidic chip, upper parallel pole is by the detecting electrode plate of upper parallel pole and the detection tabs group of upper parallel pole
Into lower parallel pole is made up of the detecting electrode plate of lower parallel pole and the detection tabs of lower parallel pole, upper parallel pole
The detecting electrode plate of detecting electrode plate and lower parallel pole is set in qually spaced on the bottom surface of runner, be parallel to each other and with flowing side
To vertical, the detection tabs of the detection tabs of upper parallel pole and lower parallel pole are arranged on outside runner, and respectively with it is upper parallel
The detecting electrode plate of electrode, the detecting electrode plate of lower parallel pole are connected；The plane wave conduction pectinate electrodes includes upper plane
Waveguide type pectinate electrodes and lower plane waveguide type pectinate electrodes, it is symmetricly set on the both sides of micro-fluidic chip, upper slab guide
Formula pectinate electrodes is by the detecting electrode plate of upper plane wave conduction pectinate electrodes and the detection tabs of upper plane wave conduction pectinate electrodes
Composition, lower plane waveguide type pectinate electrodes by lower plane waveguide type pectinate electrodes detecting electrode plate and lower plane waveguide type comb
The detection tabs composition of electrode, the inspection of the detecting electrode plate and lower plane waveguide type pectinate electrodes of upper plane wave conduction pectinate electrodes
Survey battery lead plate to be set in qually spaced on the bottom surface of runner, be parallel to each other and vertical with flow direction, upper plane wave conduction comb electricity
The detection tabs of pole and the detection tabs of lower plane waveguide type pectinate electrodes are arranged on outside runner, and respectively with upper plane wave conduction
The detecting electrode plate of pectinate electrodes is connected with the detecting electrode plate of lower plane waveguide type pectinate electrodes；The capacitive dielectric electrode
Including upper capacitive dielectric electrode and lower capacitive dielectric electrode, it is symmetricly set on the both sides of micro-fluidic chip, upper condenser type
Dielectric electrode is made up of the detecting electrode plate of upper capacitive dielectric electrode and the detection tabs of upper capacitive dielectric electrode, lower electric capacity
Formula dielectric electrode is made up of the detecting electrode plate of lower capacitive dielectric electrode and the detection tabs of lower capacitive dielectric electrode, and on
The detecting electrode plate of the detecting electrode plate of capacitive dielectric electrode and lower capacitive dielectric electrode is symmetricly set on runner inner surface
On, the detection tabs of the detection tabs of upper capacitive dielectric electrode and lower capacitive dielectric electrode are arranged on outside runner, and respectively
It is connected with the detecting electrode plate of upper capacitive dielectric electrode and the detecting electrode plate of lower capacitive dielectric electrode；The optical fiber through hole
The side of micro-fluidic chip is arranged on, for spectral detection.
The high-purity plunger piston type micro sampling pump, including：Guide rail, sliding support, plunger pump enters fluid catheter, goes out liquid and leads
Pipe, liquid outlet check valve, inlet check valve, spring, cam and motor.Wherein, guide rail be horizontally disposed with, and with sliding support
Bottom is connected, and is moved left and right so that sliding support is realized on guide rail；The top of sliding support is provided with plunger pump, in plunger
Inlet check valve and liquid outlet check valve, the plunger at one end insertion post of plunger pump are set respectively on the inlet and liquid outlet of pump
Fill in inside pump, the other end is provided with clutch plate；Spring coaxial sleeve on plunger, the two ends of spring respectively with clutch plate and plunger pump
It is in contact；Cam is fixed with the motor fixed placement, its drive shaft.When in use, control slide block support on guide rail to
One end distance is moved right, the clutch plate of plunger is in contact with cam, motor is then turned on, the drive shaft band moving cam of motor turns
It is dynamic, cam depresses plunger, and the tension of spring is combined, plunger is produced reciprocating motion inside plunger pump, so that post
The volume of plug pump interior sealing working volumes changes to produce suction, to realize from fluid catheter liquid draw body is entered, is led from liquid is gone out
Pipe discharges liquid.
The eluate conduit of the high-purity plunger piston type micro sampling pump with the liquid inlet of micro-fluidic chip is closed is connected, miniflow
The camera aperture of control chip is connected by microlens with micro- bloom spectrometer, is realized to the micro- high spectrum image information of microorganism
Collection；Capacitive dielectric electrode, plane wave conduction pectinate electrodes and the parallel pole of micro-fluidic chip connect with dielectric spectrometer respectively
Connect, realize and the dielectric property of microorganism is detected；The optical fiber through hole of micro-fluidic chip is connected with fiber spectrometer, realization pair
The spectral characteristic of microorganism is detected.Micro- bloom spectrometer, dielectric spectrometer and fiber spectrometer are connected with computer respectively, enter
Row data communication.
In the use of the present invention, adjust the sample introduction precision of high-purity plunger piston type micro sampling pump first, and open motor,
High-purity plunger piston type micro sampling pump is set to start working, high-purity plunger piston type micro sampling pump sucks microorganism sample from its liquid inlet
Liquid, and import micro-fluidic chip from liquid outlet by microorganism sample liquid.The background light source of micro-fluidic chip is then turned on, through backlight
Hole, provides background light source, and open micro- bloom spectrometer, and dielectric spectrometer and light for the micro- bloom spectrometer of micro-fluidic chip
Optical fiber spectrograph, makes it all start working, and completes the collection to each parameter of microorganism sample liquid, finally sends the parameter of collection to
Computer.
In the inventive solutions, it is embedded with force-measuring sensing in the clutch plate of the high-purity plunger piston type micro sampling pump
Device, preferably carries out embedded setting force cell in the center of clutch plate and the outer face of cam contact, and the dynamometry is passed
Sensor is connected with controller, and the controller is connected with motor, wherein：The force cell, it is convex for gathering motor drive
During wheel rotation, cam is in contact the pressure signal of generation with clutch plate, and by the pressure signal transmission of collection to controller；It is described
Controller, the pressure signal for receiving force cell conveying, and by this pressure signal, carried out with the pressure signal of setting
Compare, when two pressure signals are equal or the pressure signal of force cell conveying reaches 90-the 95% of setting pressure signal
When, control signal is sent to motor, so that motor is reversely rotated, clutch plate is left with band moving cam；When force cell conveying
Pressure signal when being zero, control signal is sent to motor, so that motor is rotated forward, to drive cam clutch plate.
In the inventive solutions, the motor model of the high-purity plunger piston type micro sampling pump
UTmotor35HB35 or star section 86BYG250A86.
In the inventive solutions, the camera aperture of the micro-fluidic chip and backlight hole are sealed using quartz plate.
In the inventive solutions, the dielectric spectrometer selects Novocontrol broadband dielectric spectroscopy instrument.
In the inventive solutions, the fiber spectrometer selects the fiber spectrometers of Maya 2000.
In the inventive solutions, the micro- bloom spectrometer selects the micro- bloom spectrometers of HScamera-ES.
In the inventive solutions, the quantity of the detecting electrode plate of the upper parallel pole is 1-3；Under described
The detecting electrode plate of parallel pole is 1-3.
In the inventive solutions, the quantity of the detecting electrode plate of the upper plane wave conduction pectinate electrodes is 2-3
It is individual；The quantity of the detecting electrode plate of the lower plane waveguide type pectinate electrodes is 2-3.
In the inventive solutions, the optical fiber through hole is arranged on the middle position of micro-fluidic chip side.
In the inventive solutions, camera aperture and the backlight hole is sealed using quartz plate.
Carrying out in use, accurately being controlled to the sample introduction of microorganism test solution using high-purity plunger piston type micro sampling pump
System so that in whole test process stabilised microorganism sample liquid inlet amount so that microorganism detection test solution from liquid inlet enter,
By microchannel, from liquid outlet outflow.Camera aperture is connected with micro- bloom spectrometer, and opens background light source, through backlight hole
Bias light is provided for micro- bloom spectrometer, to realize the collection to the micro- high spectrum image information of microorganism；Capacitive dielectric electricity
Pole, plane wave conduction pectinate electrodes and parallel pole connect dielectric spectra test equipment respectively, realize the dielectric property to microorganism
Detected with quantity；Optical fiber through hole is connected with fiber spectrometer, realizes and the spectral characteristic of microorganism is detected.
Compared with prior art, the present invention is carried out by high-purity plunger piston type micro sampling pump to the sample introduction of microorganism test solution
Accurate control, and realize and gathered to the microscopic image information of microorganism in liquid, and spectral characteristic information to microorganism and
The collection of dielectric property information, so as to realize microorganism multiple information synchronous acquisition, improves detection efficiency and accuracy of detection.
Brief description of the drawings
Fig. 1 is structural representation of the invention；
Fig. 2 for the present invention in micro-fluidic chip structural representation；
Fig. 3 is Fig. 2 top view；
Fig. 4 is Fig. 2 upward view；
Fig. 5 is Fig. 2 sectional view；
Fig. 6 for the present invention in high-purity plunger piston type micro sampling pump embodiment one structural representation；
Fig. 7 is Fig. 6 A to schematic diagram；
Fig. 8 for the present invention in high-purity plunger piston type micro sampling pump embodiment two structural representation；
Fig. 9 is Fig. 8 A to schematic diagram.
Wherein, 1 is liquid outlet, and 2-1 is upper capacitive dielectric electrode, and 2-2 is lower capacitive dielectric electrode, and 2-3 is upper electricity
The detecting electrode plate of appearance formula dielectric electrode, 2-4 is the detecting electrode plate of lower capacitive dielectric electrode, and 2-5 is upper capacitive dielectric
The detection tabs of electrode, 2-6 is the detection tabs of lower capacitive dielectric electrode, and 3-1 is upper plane wave conduction pectinate electrodes, 3-2
For lower plane waveguide type pectinate electrodes, 3-3 is the detecting electrode plate of upper plane wave conduction pectinate electrodes, and 3-4 is lower plane waveguide
The detecting electrode plate of formula pectinate electrodes, 3-5 is the detection tabs of upper plane wave conduction pectinate electrodes, and 3-6 is lower plane waveguide type
The detection tabs of pectinate electrodes, 4-1 is upper parallel pole, and 4-2 is lower parallel pole, and 4-3 is the detecting electrode of upper parallel pole
Plate, 4-4 is the detecting electrode plate of lower parallel pole, and 4-5 is the detection tabs of upper parallel pole, and 4-6 is the inspection of lower parallel pole
Joint is surveyed, 5 be liquid inlet, and 6 be backlight hole, and 7 be camera aperture, and 8 be optical fiber through hole, and 9 be guide rail, and 10 be sliding support, and 11 be post
Pump is filled in, 12 be liquid outlet check valve, and 13 be plunger, and 14 be spring, and 15 be cam, and 16 be motor, and 17 be inlet check valve, 18
It is that, into fluid catheter, 20 be clutch plate, and 21 be force cell for eluate conduit, 19.
Embodiment
Technical scheme is further illustrated with reference to specific embodiment.
Referring to accompanying drawing 1, a kind of microorganism multi-parameter comprehensive test platform, including, high-purity plunger piston type micro sampling pump is micro-
Fluidic chip, microlens, micro- bloom spectrometer, computer, fiber spectrometer, dielectric spectrometer.
Referring to accompanying drawing 2-5, the micro-fluidic chip, including：Microchannel, capacitive dielectric electrode, plane wave conduction comb
Formula electrode, parallel pole, optical fiber through hole, backlight hole and camera aperture.
The micro-fluidic chip is on the whole sealing cavity structure, and it is internally provided with microchannel, in microchannel
Two ends are respectively arranged with liquid inlet 5 and liquid outlet 1；Direction along liquid inlet to liquid outlet is disposed with camera aperture 7, backlight hole
6th, parallel pole, plane wave conduction pectinate electrodes and capacitive dielectric electrode；Camera aperture and the backlight hole is symmetricly set on micro-
The front and back of fluidic chip, the image information of the microorganism in runner can be gathered by camera aperture microscopic system, is passed through
Backlight hole, background light source can shine into microchannel, so as to provide background light source for microscopic system, camera aperture and the backlight hole is equal
Sealed using quartz plate；
The parallel pole includes upper parallel pole 4-1 and lower parallel pole 4-2, and it is symmetricly set on micro-fluidic chip
Both sides, upper parallel pole is made up of the detecting electrode plate 4-3 of upper parallel pole and the detection tabs 4-5 of upper parallel pole, lower flat
Row electrode is made up of the detecting electrode plate 4-4 of lower parallel pole and the detection tabs 4-6 of lower parallel pole, the inspection of upper parallel pole
The detecting electrode plate 4-4 for surveying battery lead plate 4-3 and lower parallel pole is set in qually spaced on the bottom surface of runner, be parallel to each other and with stream
Dynamic direction is vertical, and the detection tabs 4-6 of the detection tabs 4-5 of upper parallel pole and lower parallel pole is arranged on outside runner, and point
The detecting electrode plate 4-4 of detecting electrode plate 4-3, lower parallel pole not with upper parallel pole are connected；
The plane wave conduction pectinate electrodes includes upper plane wave conduction pectinate electrodes 3-1 and lower plane waveguide type comb electricity
Pole 3-2, it is symmetricly set on the both sides of micro-fluidic chip, and upper plane wave conduction pectinate electrodes is by upper plane wave conduction pectinate electrodes
Detecting electrode plate 3-3 and upper plane wave conduction pectinate electrodes detection tabs 3-5 composition, lower plane waveguide type pectinate electrodes by
The detecting electrode plate 3-4 of lower plane waveguide type pectinate electrodes and the detection tabs 3-6 compositions of lower plane waveguide type pectinate electrodes, on
The detecting electrode plate 3-3 of plane wave conduction pectinate electrodes and the detecting electrode plate 3-4 of lower plane waveguide type pectinate electrodes are equidistant
It is arranged on the bottom surface of runner, is parallel to each other and vertical with flow direction, the detection tabs 3-5 of upper plane wave conduction pectinate electrodes
Be arranged on the detection tabs 3-6 of lower plane waveguide type pectinate electrodes outside runner, and respectively with upper plane wave conduction pectinate electrodes
Detecting electrode plate 3-3 be connected with the detecting electrode plate 3-4 of lower plane waveguide type pectinate electrodes；
The capacitive dielectric electrode includes upper capacitive dielectric electrode 2-1 and lower capacitive dielectric electrode 2-2, and its is symmetrical
It is arranged on the both sides of micro-fluidic chip, upper capacitive dielectric electrode is by the detecting electrode plate 2-3 of upper capacitive dielectric electrode and upper
Capacitive dielectric electrode detection tabs 2-5 composition, lower capacitive dielectric electrode by lower capacitive dielectric electrode detecting electrode
Plate 2-4 and lower capacitive dielectric electrode detection tabs 2-6 composition, and the detecting electrode plate 2-3 of upper capacitive dielectric electrode and
The detecting electrode plate 2-4 of lower capacitive dielectric electrode is symmetricly set on runner inner surface, the detection of upper capacitive dielectric electrode
The detection tabs 2-6 of joint 2-5 and lower capacitive dielectric electrode is arranged on outside runner, and respectively with upper capacitive dielectric electrode
Detecting electrode plate 2-3 is connected with the detecting electrode plate 2-4 of lower capacitive dielectric electrode；
The optical fiber through hole 8 is arranged on the side of micro-fluidic chip, for spectral detection.
The quantity of the detecting electrode plate of the upper parallel pole is 1；The detecting electrode plate of the lower parallel pole is 1
It is individual.The quantity of the detecting electrode plate of the upper plane wave conduction pectinate electrodes is 2；The lower plane waveguide type pectinate electrodes
The quantity of detecting electrode plate is 3.The optical fiber through hole is arranged on the middle position of micro-fluidic chip side.
Referring to accompanying drawing 6-9, the high-purity plunger piston type micro sampling pump includes following two embodiments：
Embodiment one：
With reference to shown in accompanying drawing 6 and 7, high-purity plunger piston type micro sampling pump, including：Guide rail, sliding support, plunger pump enters
Fluid catheter, eluate conduit, liquid outlet check valve, inlet check valve, spring, cam and motor.
The guide rail 9 is horizontally disposed with, and is connected with the bottom of sliding support 10, so that sliding support realizes a left side on guide rail
Move right；It is provided with the top of sliding support and is provided with inlet on plunger pump 11, plunger pump and liquid outlet (is not marked in figure
Go out), connected respectively into fluid catheter 19 and eluate conduit 18, and on fluid catheter and eluate conduit is entered at inlet and liquid outlet
Inlet check valve 17 and liquid outlet check valve 12 are set respectively, and preferably inlet and liquid outlet is vertically set；Plunger
Inside one end insertion plunger pump of the plunger 13 of pump, the other end is provided with clutch plate 20；The coaxial sleeve of spring 14 is on plunger, spring
Two ends be in contact respectively with clutch plate and plunger pump；Cam 15, horse are fixed with the fixed placement of motor 16, its drive shaft
Up to model UTmotor35HB35 or star section 86BYG250A86, and the drive shaft of motor is vertical upward；Inlet and going out
Connected respectively into fluid catheter 19 and eluate conduit 18 at liquid mouthful, and inlet list is set on fluid catheter and eluate conduit is entered respectively
To valve and liquid outlet check valve, preferably inlet and liquid outlet is vertically set.
High-purity plunger piston type micro sampling pump carry out in use, control slide block support moves right on guide rail one end away from
From making clutch plate be in contact with cam, be then turned on motor, the drive shaft of motor drives cam rotation, and cam is pushed away when rotated
Detachment plywood and pressuring spring, while making clutch plate promote plunger, make plunger produce reciprocating motion inside plunger pump, so that
The volume of plunger pump inner sealing working volumes changes to produce suction, to realize from entering fluid catheter liquid draw body, from going out
Fluid catheter discharges liquid.Cam leaves clutch plate when rotated, due to the rebound effect of spring, and clutch plate is pushed back original by spring
Position, and drive plunger to move.Because motor and guide rail are fixedly installed, and plunger pump is fixed on and can moved along guide rail
Sliding support on, therefore by adjusting position of the sliding support on guide rail, you can realize clutch plate and motor-cam it
Between distance so that change plunger inside plunger pump stroke and reach the mesh that micro adjustment is carried out to sample introduction fluid flow
's.
Embodiment two：
With reference to shown in accompanying drawing 8 and 9, high-purity plunger piston type micro sampling pump, structure and embodiment one are basically identical, in clutch
Force cell is embedded with plate, embedded setting dynamometry is preferably carried out in the center of clutch plate and the outer face of cam contact passes
Sensor, the force cell is connected with controller, and the controller is connected with motor, wherein：
The force cell, when being rotated for gathering motor band moving cam, cam is in contact the pressure of generation with clutch plate
Force signal, and by the pressure signal transmission of collection to controller；
The controller, the pressure signal for receiving force cell conveying, and by this pressure signal, with setting
Pressure signal is compared, when two pressure signals are equal or the pressure signal of force cell conveying reaches setting pressure letter
Number 90-95% when, send control signal to motor so that motor reversely rotate, with band moving cam leave clutch plate；Work as survey
When the pressure signal of force snesor conveying is zero, control signal is sent to motor, so that motor is rotated forward, is squeezed with band moving cam
Press clutch plate.
High-purity plunger piston type micro sampling pump carry out in use, control slide block support moves right on guide rail one end away from
From and it is fixed, clutch plate is in contact with cam, be then turned on motor, the drive shaft drive cam rotation of motor, when rotated
Cam pushes clutch plate and pressuring spring, while making clutch plate promote plunger, plunger is produced reciprocating motion inside plunger pump,
So that the volume of plunger pump inner sealing working volumes changes to produce suction, to realize from entering fluid catheter liquid draw
Body, liquid is discharged from eluate conduit.Cam leaves clutch plate when rotated, and due to the rebound effect of spring, clutch plate is by spring
Home position is pushed back, and drives plunger to move.After cam completes a rotation round, you can pass through force cell
Acquisition function, when being informed in rotation round, the scope (i.e. maximum effect force value) of the intermolecular forces of cam and clutch plate, due to
Slider bracket position is fixed, and pressure signal is preset in the controller, and the pressure gathered constantly using force cell is believed
Number contrasted with preset value, when meeting control and requiring, i.e. control motor reversely or is rotated forward, band moving cam leave or
Person extrudes clutch plate.So just can be by adjusting slider bracket position and cam degree, to the flow of plunger pump
It is controlled.
With reference to accompanying drawing, the eluate conduit of the high-purity plunger piston type micro sampling pump and the liquid inlet of micro-fluidic chip are closed
It is connected, the camera aperture of micro-fluidic chip is connected by microlens with micro- bloom spectrometer, realizes to the micro- EO-1 hyperion of microorganism
The collection of image information；Capacitive dielectric electrode, plane wave conduction pectinate electrodes and the parallel pole of micro-fluidic chip respectively with
Dielectric spectrometer is connected, and realizes and the dielectric property of microorganism is detected；The optical fiber through hole and fiber spectrometer of micro-fluidic chip
It is connected, realizes and the spectral characteristic of microorganism is detected.Micro- bloom spectrometer, dielectric spectrometer and fiber spectrometer are respectively at meter
Calculation machine is connected, and enters row data communication.
The dielectric spectrometer model Novocontrol broadband dielectric spectroscopy instrument.
The fiber spectrometers of fiber spectrometer model Maya 2000.
The micro- bloom spectrometer model HScamera-ES.
In the use of the present invention, adjust the sample introduction precision of high-purity plunger piston type micro sampling pump first, and open motor,
Make high-purity plunger piston type micro sampling pump start working so that in whole test process stabilised microorganism sample liquid inlet amount,
High-purity plunger piston type micro sampling pump sucks microorganism sample liquid from its liquid inlet, and imports miniflow from liquid outlet by microorganism sample liquid
Control chip.The background light source of micro-fluidic chip is then turned on, is the micro- bloom spectrometer of micro-fluidic chip and aobvious through backlight hole
Micro lens provides background light source, and opens micro- bloom spectrometer, and dielectric spectrometer and fiber spectrometer, it is all started work
Make, complete the collection to each parameter of microorganism sample liquid, finally send the parameter of collection to computer.
Exemplary description is done to the present invention above, it should explanation, in the situation for the core for not departing from the present invention
Under, any simple deformation, modification or other skilled in the art can not spend the equivalent substitution of creative work equal
Fall into protection scope of the present invention.
Claims (10)
1. the application method of microorganism multi-parameter comprehensive test platform, it is characterised in that use high-purity plunger piston type micro sample introduction
Pump is accurately controlled the sample introduction of microorganism test solution so that in whole test process stabilised microorganism sample liquid inlet amount,
Microorganism detection test solution enters from the liquid inlet of microchannel, by microchannel, is flowed out from the liquid outlet of microchannel, at this
During one, while the collection to the micro- high spectrum image information of microorganism is realized using microlens and micro- bloom spectrometer,
It is connected using optical fiber through hole with fiber spectrometer, realizes and the spectral characteristic of microorganism is detected, is utilized with capacitive dielectric
Electrode, plane wave conduction pectinate electrodes and parallel pole connect dielectric spectra test equipment respectively, realize special to the dielectric of microorganism
Property and quantity detected, wherein microorganism multi-parameter comprehensive test platform, including, it is high-purity plunger piston type micro sampling pump, micro-
Fluidic chip, microlens, micro- bloom spectrometer, computer, fiber spectrometer, dielectric spectrometer：
Micro-fluidic chip, including：Microchannel, capacitive dielectric electrode, plane wave conduction pectinate electrodes, parallel pole, optical fiber
Through hole, backlight hole and camera aperture, micro-fluidic chip are on the whole sealing cavity structure, and it is internally provided with microchannel, micro-
The two ends of circulation road are respectively arranged with liquid inlet and liquid outlet；Direction along liquid inlet to liquid outlet be disposed with camera aperture,
Backlight hole, parallel pole, plane wave conduction pectinate electrodes and capacitive dielectric electrode；Camera aperture and the backlight hole is symmetrical arranged
In the front and back of micro-fluidic chip；The parallel pole includes upper parallel pole and lower parallel pole, and it is symmetricly set on
The both sides of micro-fluidic chip, upper parallel pole is by the detecting electrode plate of upper parallel pole and the detection tabs group of upper parallel pole
Into lower parallel pole is made up of the detecting electrode plate of lower parallel pole and the detection tabs of lower parallel pole, upper parallel pole
The detecting electrode plate of detecting electrode plate and lower parallel pole is set in qually spaced on the bottom surface of runner, be parallel to each other and with flowing side
To vertical, the detection tabs of the detection tabs of upper parallel pole and lower parallel pole are arranged on outside runner, and respectively with it is upper parallel
The detecting electrode plate of electrode, the detecting electrode plate of lower parallel pole are connected；The plane wave conduction pectinate electrodes includes upper plane
Waveguide type pectinate electrodes and lower plane waveguide type pectinate electrodes, it is symmetricly set on the both sides of micro-fluidic chip, upper slab guide
Formula pectinate electrodes is by the detecting electrode plate of upper plane wave conduction pectinate electrodes and the detection tabs of upper plane wave conduction pectinate electrodes
Composition, lower plane waveguide type pectinate electrodes by lower plane waveguide type pectinate electrodes detecting electrode plate and lower plane waveguide type comb
The detection tabs composition of electrode, the inspection of the detecting electrode plate and lower plane waveguide type pectinate electrodes of upper plane wave conduction pectinate electrodes
Survey battery lead plate to be set in qually spaced on the bottom surface of runner, be parallel to each other and vertical with flow direction, upper plane wave conduction comb electricity
The detection tabs of pole and the detection tabs of lower plane waveguide type pectinate electrodes are arranged on outside runner, and respectively with upper plane wave conduction
The detecting electrode plate of pectinate electrodes is connected with the detecting electrode plate of lower plane waveguide type pectinate electrodes；The capacitive dielectric electrode
Including upper capacitive dielectric electrode and lower capacitive dielectric electrode, it is symmetricly set on the both sides of micro-fluidic chip, upper condenser type
Dielectric electrode is made up of the detecting electrode plate of upper capacitive dielectric electrode and the detection tabs of upper capacitive dielectric electrode, lower electric capacity
Formula dielectric electrode is made up of the detecting electrode plate of lower capacitive dielectric electrode and the detection tabs of lower capacitive dielectric electrode, and on
The detecting electrode plate of the detecting electrode plate of capacitive dielectric electrode and lower capacitive dielectric electrode is symmetricly set on runner inner surface
On, the detection tabs of the detection tabs of upper capacitive dielectric electrode and lower capacitive dielectric electrode are arranged on outside runner, and respectively
It is connected with the detecting electrode plate of upper capacitive dielectric electrode and the detecting electrode plate of lower capacitive dielectric electrode；It is described to be used for spectrum
The optical fiber through hole of detection is arranged on the side of micro-fluidic chip；
High-purity plunger piston type micro sampling pump, including：Guide rail, sliding support, plunger pump, enter fluid catheter, eluate conduit, liquid outlet
Check valve, inlet check valve, spring, cam and motor, wherein, guide rail is horizontally disposed with, and is connected with the bottom of sliding support；
The top of sliding support is provided with plunger pump, set respectively on the inlet and liquid outlet of plunger pump inlet check valve and
Inside liquid outlet check valve, the plunger at one end insertion plunger pump of plunger pump, the other end is provided with clutch plate；Spring coaxial sleeve is in post
Beyond the Great Wall, the two ends of spring are in contact with clutch plate and plunger pump respectively；It is fixed with the motor fixed placement, its drive shaft convex
Wheel.
The eluate conduit of high-purity plunger piston type micro sampling pump with the liquid inlet of micro-fluidic chip is closed is connected, micro-fluidic chip
Camera aperture is connected by microlens with micro- bloom spectrometer；Capacitive dielectric electrode, the plane wave conduction comb of micro-fluidic chip
Formula electrode and parallel pole are connected with dielectric spectrometer respectively；The optical fiber through hole of micro-fluidic chip is connected with fiber spectrometer；It is micro-
Bloom spectrometer, dielectric spectrometer and fiber spectrometer are connected with computer respectively, enter row data communication.
2. the application method of microorganism multi-parameter comprehensive test platform according to claim 1, it is characterised in that high-precision
Force cell is embedded with the clutch plate for spending plunger type micro-sampling pump, the force cell is connected with controller, the control
Device processed is connected with motor, wherein：The force cell, when being rotated for gathering motor band moving cam, cam and clutch plate phase
The pressure signal produced is contacted, and by the pressure signal transmission of collection to controller；The controller, for receiving force-measuring sensing
The pressure signal of device conveying, and by this pressure signal, be compared with the pressure signal of setting, when two pressure signals are equal
Or force cell conveying pressure signal reach setting pressure signal 90-95% when, send control signal to motor,
So that motor is reversely rotated, clutch plate is left with band moving cam；When the pressure signal that force cell is conveyed is zero, to motor
Control signal is sent, so that motor is rotated forward, to drive cam clutch plate.
3. the application method of microorganism multi-parameter comprehensive test platform according to claim 1, it is characterised in that the height
The motor model UTmotor35HB35 or star section 86BYG250A86 of precision plunger type micro-sampling pump.
4. the application method of microorganism multi-parameter comprehensive test platform according to claim 1, it is characterised in that given an account of
Electric spectrometer is Novocontrol broadband dielectric spectroscopy instrument.
5. the application method of microorganism multi-parameter comprehensive test platform according to claim 1, it is characterised in that the light
Optical fiber spectrograph is the fiber spectrometers of Maya 2000.
6. the application method of microorganism multi-parameter comprehensive test platform according to claim 1, it is characterised in that described aobvious
Micro- bloom spectrometer model HScamera-ES.
7. the application method of microorganism multi-parameter comprehensive test platform according to claim 1, it is characterised in that described micro-
The camera aperture of fluidic chip and backlight hole are sealed using quartz plate.
8. the application method of microorganism multi-parameter comprehensive test platform according to claim 1, it is characterised in that the light
Fine through hole is arranged on the middle position of micro-fluidic chip side.
9. the application method of microorganism multi-parameter comprehensive test platform according to claim 1, it is characterised in that on described
The quantity of the detecting electrode plate of parallel pole is 1-3；The detecting electrode plate of the lower parallel pole is 1-3.
10. the application method of microorganism multi-parameter comprehensive test platform according to claim 1, it is characterised in that described
The quantity of the detecting electrode plate of upper plane wave conduction pectinate electrodes is 2-3；The detection of the lower plane waveguide type pectinate electrodes
The quantity of battery lead plate is 2-3.
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