CN105631245B - 一种用于鉴定美洲黑杨优良无性系的专用引物及其鉴定方法 - Google Patents
一种用于鉴定美洲黑杨优良无性系的专用引物及其鉴定方法 Download PDFInfo
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- CN105631245B CN105631245B CN201610011808.9A CN201610011808A CN105631245B CN 105631245 B CN105631245 B CN 105631245B CN 201610011808 A CN201610011808 A CN 201610011808A CN 105631245 B CN105631245 B CN 105631245B
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- 241000218978 Populus deltoides Species 0.000 title claims abstract description 36
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- 230000003321 amplification Effects 0.000 claims description 13
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 13
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- 238000012408 PCR amplification Methods 0.000 claims description 7
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- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 3
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
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- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
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- Bioinformatics & Computational Biology (AREA)
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- Spectroscopy & Molecular Physics (AREA)
- Theoretical Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
名称 | 上游引物(5’-3’) | 下游引物(5’-3’) |
NJFUP-poly01 | GACACCGTTTCTTTTCTGAG | ACCAGATCTTCATCTTCCAA |
NJFUP-poly02 | TCGAATAGCTGGGATACTTC | TATCACCCGAACAAAAACTC |
NJFUP-poly05 | CCTTAGCCTTCTCACACAAC | GGTCTCCATTTTAGCTGTCA |
NJFUP-poly06 | GCCCAAACTCTTATTTGATG | TGGTGGAGGCTAGGATAGTA |
NJFUP-poly07 | ATTGCTCTTGCAGAAATCAT | GGATACCAAGTCATCGGTAG |
NJFUP-poly10 | AGAACTTGTGAGGCAGGTAA | AAAGCTCTCCCCTAACAAAG |
Claims (2)
Priority Applications (1)
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CN201610011808.9A CN105631245B (zh) | 2016-01-08 | 2016-01-08 | 一种用于鉴定美洲黑杨优良无性系的专用引物及其鉴定方法 |
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CN201610011808.9A CN105631245B (zh) | 2016-01-08 | 2016-01-08 | 一种用于鉴定美洲黑杨优良无性系的专用引物及其鉴定方法 |
Publications (2)
Publication Number | Publication Date |
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CN105631245A CN105631245A (zh) | 2016-06-01 |
CN105631245B true CN105631245B (zh) | 2018-09-25 |
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CN201610011808.9A Expired - Fee Related CN105631245B (zh) | 2016-01-08 | 2016-01-08 | 一种用于鉴定美洲黑杨优良无性系的专用引物及其鉴定方法 |
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CN (1) | CN105631245B (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN113249509B (zh) * | 2020-02-07 | 2023-09-01 | 南京林业大学 | 用于美洲黑杨和小叶杨种间杂交子代的鉴别引物和鉴定方法 |
CN111534628B (zh) * | 2020-04-29 | 2022-05-31 | 南京林业大学 | 用于鉴定美洲黑杨性别的ssr分子标记引物组及其应用 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102533993A (zh) * | 2011-12-28 | 2012-07-04 | 南京林业大学 | 一种多倍体杨树的筛选方法及其专用引物 |
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- 2016-01-08 CN CN201610011808.9A patent/CN105631245B/zh not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102533993A (zh) * | 2011-12-28 | 2012-07-04 | 南京林业大学 | 一种多倍体杨树的筛选方法及其专用引物 |
Non-Patent Citations (1)
Title |
---|
美洲黑杨无性系AFLP指纹分析;尹佟明等;《植物学报》;19981231;第40卷(第8期);第778-780页 * |
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