CN105616184A - Hyaluronic acid-polypeptide bonded conjugate mask and preparation method thereof - Google Patents
Hyaluronic acid-polypeptide bonded conjugate mask and preparation method thereof Download PDFInfo
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- CN105616184A CN105616184A CN201410591484.1A CN201410591484A CN105616184A CN 105616184 A CN105616184 A CN 105616184A CN 201410591484 A CN201410591484 A CN 201410591484A CN 105616184 A CN105616184 A CN 105616184A
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- hyaluronic acid
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- peptide bond
- skin
- acid peptide
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- 238000002360 preparation method Methods 0.000 title claims description 14
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- 229960003160 hyaluronic acid Drugs 0.000 claims abstract description 73
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims abstract description 71
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 50
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- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 12
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Abstract
The invention discloses a hyaluronic acid-polypeptide bonded conjugate mask, which comprises the following ingredients (by weight): 0.1-20 parts of hyaluronic acid-polypeptide bonded conjugate, 0.1-10.0 parts of a skin whitening agent, 2.0-20.0 parts of an emulsifier, 0.5-20.0 parts of a cutin softener, 1.0-15.0 parts of a skin anti-wrinkling agent, 0.1-5.0 parts of a flavoring agent, 0.1-10.0 parts of glycerin and the rest of water. The mask composition contains polypeptide growth factors for preserving moisture, whitening, removing freckles, removing wrinkles and repairing scars, such as fibroblast growth factor, epithelial growth factor, vascular endothelial growth factor and the like. Those polypeptide growth factors and hyaluronic acid with low molecular weight are bonded and are released through a reversible chemical bond during the using process to be absorbed by face skin. Acting time is long, and cosmetic result is obvious.
Description
Technical field
The present invention relates to skin care field, be specially a kind of hyaluronic acid peptide bond object plane film and preparation method thereof.
Background technology
Facial film is a kind of cosmetology product conventional at present, principle is the of short duration time utilizing and covering face, by being temporarily isolating air and the pollution in the external world, improve skin temperature, the pore expansion of skin, promote sweat gland secretion and metabolism, the oxygen content making skin rises, skin is conducive to get rid of the oil substances of the metabolic product of epidermis cell and accumulation, moisture content in facial film penetrates into the horny layer of skin epidermis, skin becomes soft, and light is with elasticity naturally, reaches the effect of beautifying and anti-aging. Facial film kind on domestic market is very many, various compositions in facial film, impart the effects such as facial film whitening antioxygen, moisturizing moisturizing, Firm skin crease-resistant, sensitive, whitening and moisturizing, moisturizing isolation such as materials such as hyaluronic acid, superoxide dismutase, fibroblast growth factors, thus be subject to liking of numerous people seeking beauty.
Hyaluronic acid, has another name called hyaluronic acid, is a kind of acid mucopolysaccharide. It is the important foundation material that skin water is tender, itself is also a kind of composition of human body, and it has special water retention, 100 times of deal more up to itself weight, for instance: the pure hyaluronic acid aqueous solution of 2% can firmly hold 98% moisture. It is the material that in the nature having now been found that, moisture retention is best, is referred to as desirable nature moisturizing factor (Naturalmoisturizingfactor, NMF). With other nutritional labelings with the use of, it is possible to play promote alimentation better effect. Hyaluronic acid demonstrates multiple important physiological function with molecular structure and the physicochemical property of its uniqueness in body, such as lubricating joint, the permeability of adjustment blood vessel wall, adjustment protein, Water-Electrolyte diffusion and operating, promotion wound healing etc. Macromole hyaluronic acid (molecular weight ranges 1800000��2200000) can form thin film one layer ventilative at skin surface, makes skin smooth moisten, and can intercept alien bacteria, dust, ultraviolet intrusion, protects the skin from infringement; Middle molecule hyaluronic acid (molecular weight ranges 1000000��1800000) can compact skin, permanent moisturizing; Micromolecule hyaluronic acid (molecular weight ranges 400000��1000000) can penetrate into corium, there is mild dilation blood capillary, increase blood circulation, improve intermediate supersession, promote skin-nourishing Absorption, there is stronger wrinkle-chasing function, skin elasticity, delay skin aging can be increased.
Palmitoyl Tetrapeptide (Palmitoyltetrapeptide-3), it is a kind of peptide by 4 Amino acid synthesis, is widely used in cosmetics and cosmetics, it is possible to reduce the generation of interleukin, diminish inflammation, reach anti crease and anti senile prepared old purpose thereby through antiinflammatory edema.
Acetic acid M peptide/Palmic acid Wushengtai MatrixylAcetate/ (PalmitoylPentapeptide), for synthesis collagen protein predecessor, suppressing the generation of metalloprotein amylase, and promote collagen protein synthesis, the elastin laminin acting on skin corium recovers skin elastic. It is effectively improved wrinkle, reproduces smooth resilient skin quality. There is obvious anti-aging effects.
Acetyl group six peptides/Argireline Acetylhexapeptide-3, six amino acids the class botullnus factor combined, the class that is otherwise known as meat poisoning rhzomorph, it is possible to suppress nerve conduction, reduces muscle contraction, it is prevented that the formation of express one's feelings stricture of vagina, dynamic stricture of vagina. It is a kind of biologically active polypeptide, has moisturizing, reduces existing facial wrinkles, can effectively prevent again new wrinkle from producing, have obvious anti-aging effects.
Fibroblast growth factor (fi-broblastgrowthfactors, FGFs), fibroblast growth factor is a kind of multifunctional powerful cytokine, the formation promoting fibroblastic metabolism and collagen protein is played critical function, synthesis and the cellular metabolism of the protein of Skin Cell can be strengthened, there is skin-cell aging delaying, the reparation promoting epidermis cell and growth, make skin smooth plump. It is divided into acid fibroblast growth factor and basic fibroblast growth factor. Acid fibroblast growth factor (acidfibro-blastgrowthfactor, aFGF), i.e. aFGF, it is made up of 147 aminoacid, molecular weight is the active polypeptide of 15.3KD. Can not only promote that the generation of new cell is to substitute original cell, to reduce melanin and coloured corpuscle content in skin, improve microcirculation to accelerate the metabolism of pigment, thus reaching desalination pigment and ruddy skin effect. By skin renewal, promote epidermal elasticity, supplement skin-nourishing three approach and play the effect preventing and dispelling wrinkle. To acne of dispelling, mottle of dispelling, brighten, improve skin elasticity, damage skin repair and effectively reach 95%, to skin gloss, moisten, soft, subtract wrinkle etc. and effectively reach 93%. Basic fibroblast growth factor (basefibro-blastgrowthfactor, bFGF) bFGF is containing 155 amino acid whose polypeptide. Senile cell can being updated, thus alleviating the calmness of skin pigment, reaching the effect of whitening and speckle dispelling. The microenvironment of Growth of Cells can be improved, promote the synthesis of elastic fiber and collagen protein, make skin high resilience, make skin be in sliding tender state. Fibroblastic growth promoter can be promoted, constantly replace aged cells with new cell, have wrinkle resistant, wrinkle removal effect.
Epithelium growth factor (EGF), is a kind of active substance in human body, is made up of 53 amino, it can stimulate epithelial cell and endothelial cell growth, making new epidermis cell constantly grow up to, cortex of checkmating promotes and comes off gradually, keeps skin delicacy smooth. Promote collagen and collagenase synthesis, secretion Collagen material, hyaluronic acid and glycoprotein, regulate collagen fiber, there is skin care, strengthen skin elasticity, reduce wrinkle of skin and prevent the effect of skin aging.
VEGF (vascularendothelialgrowthfactor, VEGF), is the homodimer glycoprotein of high conservative. Article two, molecular weight respectively forms dimer for the strand of 24kDa with disulfide bond, early stage is also referred to as vascular permeability factor, vascular endothelial cell proliferation can be promoted by vasoactive endotheliocyte, increase vascular permeability, facial blood circulation can be accelerated, get rid of the toxin below skin, enable nutrient to enter skin, fully it is absorbed by the skin, makes skin become water tender, smooth. The blood Rapid Circulation of face can play the effect of wrinkle removing skin-active simultaneously, makes skin ruddy careful, except effect of pox speckle dispelling.
Superoxide dismutase (SuperoxideDismutase; SOD); it it is a kind of active substance coming from life entity; energy scavenging free radicals; epidermis cell, sebaceous gland and subcutaneous fat, big eccrine gland, elastin and fiber are played a very good protection; ensure that skin histology is metabolic is normally carried out, make skin smooth, exquisiteness, flexible, and reduce wrinkle. Thus can slow down aging not only crease-resistant, speckle dispelling, the effects such as element of discoloring, also have the effect of antiinflammatory, sun-proof, delay skin aging.
Along with the nutritional labeling contained in the progressive facial film of science and technology also gets more and more, these materials can bring the beauty functions of mystery on the one hand to facial film, but then, there is also the problem much allowing people worry. The natural collagen of the macromole added in such as facial film is difficult to be absorbed by the skin, and is not suitable for human body skin and absorbs, and water solublity and permeability are also poor; In facial film, some compositions are unstable, and cosmetic result, anti-aging effects are not good enough, and the beautification function time is short; Facial film cleaning effect is poor, the skin permeation that skin care ingredient can not go deep into, and infiltration capacity is also little, is not suitable for needing the people of the clean skin of deep layer; Even being added with a large amount of chemical preservative in some facial films, and mineral composition content is more, skin is irritant, and sensitivity skin is to be not suitable for using, it is therefore desirable to the facial film of a kind of safe, non-stimulated and broad spectrum activity.
Summary of the invention
The technical problem to be solved in the present invention is to overcome existing defect, it is provided that the preparation method of a kind of hyaluronic acid peptide bond object plane film, calculates by mass percentage, and its formula contains following components:
Hyaluronic acid peptide bond thing 0.1��20.0 part, skin whitening agent 0.1��10.0 part, emulsifying agent 2.0��20.0 parts, keratolytic 0.5��20.0 part, external skin wrinkling-preventing agent 1.0��15.0 parts, aromatic 0.1��5.0 part, glycerol 0.1��10.0 part, surplus is water.
Further, calculating by mass percentage, its formula contains following components:
Hyaluronic acid peptide bond thing 1.0��3.5 parts, skin whitening agent 2.5��5 parts, emulsifying agent 8��12 parts, keratolytic 1��4 part, external skin wrinkling-preventing agent 3��5 parts, aromatic 0.1��5 part, glycerol 2��4 parts, surplus is water.
Further; described hyaluronic acid peptide bond thing refers to that hyaluronic acid is bonded with the conjugation of one or more in fibroblast growth factor, epithelium growth factor, VEGF, superoxide dismutase, Palmitoyl Tetrapeptide, Palmic acid Wushengtai, acetyl group six peptide and obtains, and described hyaluronan molecule amount selection is prone to the micromolecule hyaluronic acid of the < 50KDa absorbed by human epidermal
Further, described a kind of hyaluronic acid peptide bond object plane film, described skin whitening agent is any one or a few in vitamin C, Oleum Hippophae, fruit acid, ascorbic acid, gallic acid, kojic acid, vitamin B3.
Further, described keratolytic lanoline, any one or a few in Oleum Helianthi, Oleum Vitis viniferae, safflower oil, Radix Oenotherae erythrosepalae oil, dissolving keratinase.
Further, described external skin wrinkling-preventing agent is any one or a few in nicotinic acid, nicotiamide, D��pantothenic acid, vitamin E.
Further, described aromatic is any one or a few in Salvia farinacea oil, Flos Rosae Rugosae quintessence oil, chamomile essential oil, Herba Lysimachiae foenum-graeci quintessence oil, Bergamot, Fructus Citri Limoniae quintessence oil, Peppermint essential oil.
Further, the preparation method of described hyaluronic acid peptide bond thing, for being 10 by 1.0��5.0g molecular weight, 000��30, the hyaluronic acid of 000 is dissolved in 80��100mlPBS buffer solution, and regulating pH is 7.0��8.0, adds the polypeptide of 100��400mg sodium metaperiodate open loop and 100��200mg, conjugation condensation reaction 8 hours, it is ensured that react completely; After gel chromatography purification, lyophilizing, nuclear magnetic resonance, NMR confirms.
The preparation method of this hyaluronic acid peptide bond object plane film, comprises the following steps:
By hyaluronic acid peptide bond thing 0.1��20 part, skin whitening agent 0.1��10 part, emulsifying agent 2��20 parts, keratolytic 0.5��20 part, adding in ultra-pure water, 40��50 DEG C stir, standby;
(2) external skin wrinkling-preventing agent 1��15 part, aromatic 0.1��5 part, glycerol 0.1��10 part, mix homogeneously, standby;
(3) join when the mixture of step (1) gained being cooled to 35 DEG C in the mixture that step (2) obtains, stir;
(4) mixture obtained to step (3) adds ultra-pure water, be 100 parts to mixture gross mass.
The invention has the beneficial effects as follows:
Comprising the polypeptide growth factor with moisturizing, whitening, speckle dispelling, reparation cicatrix in the mask composition of the present invention, these polypeptide growth factor, enough to anti-wrinkle, slow down aging. In order to make somatomedin well pass through skin absorption, polypeptide is bonded with micromolecule hyaluronic acid, while reaching skin Caring, hyaluronic water-retaining property and its can penetrate into corium, there is mild dilation blood capillary, increase blood circulation, improve intermediate supersession, promote skin-nourishing Absorption, it is possible to reach stronger wrinkle-chasing function, thus increasing skin elasticity, and then the beauty functions of delay skin aging. The facial film that hyaluronic acid makes with albumen or polypeptide peptide bond compound is relative stable, is in use discharged by reversible chemical bond and is absorbed by facial skin, and long action time, cosmetic result is obvious.
Accompanying drawing explanation
Accompanying drawing is for providing a further understanding of the present invention, and constitutes a part for description, is used for together with embodiments of the present invention explaining the present invention, is not intended that limitation of the present invention. In the accompanying drawings:
Fig. 1 is that in embodiments of the invention 4, the nuclear magnetic spectrogram of acid fibroblast growth factor and hyaluronic acid bonding product is tied;
Fig. 2 is the nuclear magnetic spectrogram of hyaluronic acid and Palmic acid Wushengtai key compound in embodiments of the invention 6.
Detailed description of the invention
Hereinafter the preferred embodiments of the present invention are illustrated, it will be appreciated that preferred embodiment described herein is merely to illustrate and explains the present invention, is not intended to limit the present invention.
Embodiment 1
A kind of hyaluronic acid peptide bond object plane film, calculates, by mass percentage containing following components:
Hyaluronic acid and superoxide dismutase key compound 0.1 part, vitamin C agent 10.0 parts, emulsifying agent 2.0 parts, lanoline 0.5 part, 1.0 parts of nicotinic acid, Salvia farinacea oil 5.0 parts, glycerol 10.0 parts, surplus is water.
Embodiment 2
A kind of hyaluronic acid peptide bond object plane film, calculates, by mass percentage containing following components:
Hyaluronic acid and Palmitoyl Tetrapeptide key compound 20.0 parts, Oleum Hippophae 0.1 part, emulsifying agent 20.0 parts, Oleum Helianthi 20.0 parts, nicotiamide 15.0 parts, Flos Rosae Rugosae quintessence oil 0.1 part, glycerol 0.1 part, surplus is water.
Embodiment 3
A kind of hyaluronic acid peptide bond object plane film, calculates, by mass percentage containing following components:
Hyaluronic acid and VEGF key compound 10.0 parts, fruit acid 5.0 parts, emulsifying agent 11.0 parts, Oleum Vitis viniferae 10.5 parts, 8.0 parts of D��pantothenic acid, chamomile essential oil 2.5 parts, glycerol 5.0 parts, surplus is water. This facial film has the function improving the anti-red blood streak of microcirculation.
Wherein, hyaluronic acid with the preparation process of the key compound of VEGF is:
The hyaluronic acid that 1.0g molecular weight is 30,000 is dissolved in 80mlPBS buffer solution (1.25%, w/v), regulating pH is 7.0, adds 200mg sodium metaperiodate open loop, adds the VEGF of 100mg, conjugation condensation reaction 8 hours, it is ensured that react completely; After gel chromatography purification, lyophilizing, nuclear magnetic resonance, NMR confirms.
The preparation method of this hyaluronic acid VEGF key compound facial film, comprises the following steps:
By hyaluronic acid and VEGF key compound 10.0 parts, fruit acid 5.0 parts, emulsifying agent 11.0 parts, Oleum Vitis viniferae 10.5 parts, adding in ultra-pure water, 40��50 DEG C stir, standby;
(2) 8.0 parts of D��pantothenic acid, chamomile essential oil 2.5 parts, glycerol 5.0 parts, mix homogeneously, standby;
(3) join when the mixture of step (1) gained being cooled to 35 DEG C in the mixture that step (2) obtains, stir;
(4) mixture obtained to step (3) adds ultra-pure water, be 100 parts to mixture gross mass.
Embodiment 4
A kind of hyaluronic acid peptide bond object plane film, calculates, by mass percentage containing following components:
Hyaluronic acid and acid fibroblast growth factor key compound 2.0 parts, 2.5 parts of ascorbic acid, emulsifying agent 8 parts, safflower oil 1 part, vitamin E 3 parts, Herba Lysimachiae foenum-graeci quintessence oil 0.1 part, glycerol 2 parts, surplus is water. This facial film is a kind of facial film with deep layer repair function.
Wherein, hyaluronic acid with the preparation process of acid fibroblast growth factor key compound is:
The hyaluronic acid that 3.5g molecular weight is 20,000 is dissolved in 90mlPBS buffer solution (3.9%, w/v), regulating pH is 7.2, adds 400mg sodium metaperiodate open loop, adds the acid fibroblast growth factor of 200mg, conjugation condensation reaction 8 hours, it is ensured that react completely; After gel chromatography purification, lyophilizing, nuclear magnetic resonance, NMR confirms. The nuclear magnetic spectrogram of acid fibroblast growth factor and hyaluronic acid bonding product is as shown in Figure 1.
Hyaluronic acid and the preparation method of acid fibroblast growth factor key compound facial film, comprise the following steps:
Hyaluronic acid and acid fibroblast growth factor key compound 2.0 parts, 2.5 parts of ascorbic acid, emulsifying agent 8 parts, safflower oil 1 part, add in ultra-pure water, 40��50 DEG C stir, standby;
(2) vitamin E 3 parts, Herba Lysimachiae foenum-graeci quintessence oil 0.1 part, glycerol 2 parts, mix homogeneously, standby;
(3) join when the mixture of step (1) gained being cooled to 35 DEG C in the mixture that step (2) obtains, stir;
(4) mixture obtained to step (3) adds ultra-pure water, be 100 parts to mixture gross mass.
Embodiment 5
A kind of hyaluronic acid peptide bond object plane film, calculates, by mass percentage containing following components:
Hyaluronic acid and epithelium growth factor key compound 3.5 parts, gallic acid 5 parts, emulsifying agent 12 parts, Radix Oenotherae erythrosepalae oil 4 parts, vitamin E 5 parts, Bergamot 5 parts, glycerol 4 parts, surplus is water. This facial film has anti-acne repair function.
Wherein, hyaluronic acid with the preparation process of epithelium growth factor key compound is:
Being dissolved in 100mlPBS buffer solution by the hyaluronic acid that 4g molecular weight is 10,000 (4%, w/v), regulating pH is 7.5, adds 400mg sodium metaperiodate open loop, adds the epithelium growth factor of 200mg, conjugation condensation reaction 8 hours, it is ensured that react completely; After gel chromatography purification, lyophilizing, nuclear magnetic resonance, NMR confirms.
Embodiment 6
A kind of hyaluronic acid peptide bond object plane film, calculates, by mass percentage containing following components:
Hyaluronic acid and Palmic acid Wushengtai key compound 3 parts, kojic acid 4 parts, emulsifying agent 8 parts, dissolve keratinase 1��4 part, nicotiamide 3 parts, Fructus Citri Limoniae quintessence oil 2 parts, glycerol 2 parts, surplus is water. The real a kind of anti-wrinkle face mask of this facial film.
Wherein, hyaluronic acid with the preparation process of Palmic acid Wushengtai key compound is:
The hyaluronic acid that 5.0g molecular weight is 25,000 is dissolved in 85mlPBS buffer solution (5.88%, w/v), regulating pH is 7.5, adds 200mg sodium metaperiodate open loop, adds the Palmic acid Wushengtai key compound of 175mg, conjugation condensation reaction 8 hours, it is ensured that react completely; After gel chromatography purification, lyophilizing, nuclear magnetic resonance, NMR confirms. The nuclear magnetic spectrogram of hyaluronic acid and Palmic acid Wushengtai key compound is as shown in Figure 2.
Embodiment 7
A kind of hyaluronic acid peptide bond object plane film, calculates, by mass percentage containing following components:
Hyaluronic acid and acetyl group six peptide bond compound 1.0 parts, vitamin B3 2.5 parts, emulsifying agent 9 parts dissolves keratinase 3 parts, and 4 parts of D��pantothenic acid, Peppermint essential oil 2 parts, glycerol 4 parts, surplus is water.
Embodiment 8
A kind of hyaluronic acid peptide bond object plane film, calculates, by mass percentage containing following components:
Hyaluronic acid and many Palmitoyl Tetrapeptide key compound 5.0 parts, Catergen .5 part, emulsifying agent 10 parts, keratolytic 2 parts, vitamin E 4 parts, Flos Rosae Rugosae quintessence oil 2 parts, glycerol 2 parts, surplus is water.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention, although the present invention being described in detail with reference to previous embodiment, for a person skilled in the art, technical scheme described in foregoing embodiments still can be modified by it, or wherein portion of techniques feature carries out equivalent replacement. All within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention.
Claims (9)
1. a hyaluronic acid peptide bond object plane film, it is characterised in that calculate by mass percentage, containing following components:
Hyaluronic acid peptide bond thing 0.1��20 part, skin whitening agent 0.1��10.0 part, emulsifying agent 2.0��20.0 parts, keratolytic 0.5��20.0 part, external skin wrinkling-preventing agent 1.0��15.0 parts, aromatic 0.1��5.0 part, glycerol 0.1��10.0 part, surplus is water.
2. a kind of hyaluronic acid peptide bond object plane film as claimed in claim 1, it is characterised in that calculate by mass percentage, containing following components:
Hyaluronic acid peptide bond thing 1.0��5.0 parts, skin whitening agent 2.5��5 parts, emulsifying agent 8��12 parts, keratolytic 1��4 part, external skin wrinkling-preventing agent 3��5 parts, aromatic 0.1��5 part, glycerol 2��4 parts, surplus is water.
3. a kind of hyaluronic acid peptide bond object plane film as claimed in claim 1 or 2; it is characterized in that; described hyaluronic acid peptide bond thing refers to that hyaluronic acid is bonded with one or more conjugation in fibroblast growth factor, epithelium growth factor, VEGF, superoxide dismutase, Palmitoyl Tetrapeptide, Palmic acid Wushengtai, acetyl group six peptide and obtains, and described hyaluronan molecule amount is less than 50KDa.
4. a kind of hyaluronic acid peptide bond object plane film as claimed in claim 1 or 2, it is characterised in that described skin whitening agent is any one or a few in vitamin C, Oleum Hippophae, fruit acid, ascorbic acid, gallic acid, kojic acid, vitamin B3.
5. hyaluronic acid peptide bond object plane film as claimed in claim 1 or 2 a kind of, it is characterised in that described keratolytic lanoline, any one or a few in Oleum Helianthi, Oleum Vitis viniferae, safflower oil, Radix Oenotherae erythrosepalae oil, dissolving keratinase.
6. a kind of hyaluronic acid peptide bond object plane film as claimed in claim 1 or 2, it is characterised in that described external skin wrinkling-preventing agent is any one or a few in nicotinic acid, nicotiamide, D��pantothenic acid, vitamin E.
7. a kind of hyaluronic acid peptide bond object plane film as claimed in claim 1 or 2, it is characterized in that, described aromatic is any one or a few in Salvia farinacea oil, Flos Rosae Rugosae quintessence oil, chamomile essential oil, Herba Lysimachiae foenum-graeci quintessence oil, Bergamot, Fructus Citri Limoniae quintessence oil, Peppermint essential oil.
8. a kind of hyaluronic acid peptide bond object plane film as claimed in claim 1, it is characterized in that, the preparation method of described hyaluronic acid peptide bond thing is, the hyaluronic acid that 1��5g molecular weight is 10,000��30,000 is dissolved in 80��100mlPBS buffer solution, regulating pH is 7.0��8.0, add the polypeptide of 100��400mg sodium metaperiodate open loop and 100��200mg, conjugation condensation reaction 8 hours, it is ensured that react completely; After gel chromatography purification, lyophilizing, nuclear magnetic resonance, NMR confirms.
9. the preparation method of a hyaluronic acid peptide bond object plane film, it is characterised in that comprise the following steps:
Hyaluronic acid peptide bond thing 0.1��20 part, skin whitening agent 0.1��10.0 part, emulsifying agent 2.0��20.0 parts, keratolytic 0.5��20.0 part, add in ultra-pure water, stir at 40��50 DEG C, standby;
(2) external skin wrinkling-preventing agent 1.0��15 parts, aromatic 0.1��5 part, glycerol 0.1��10 part, mix homogeneously, standby;
(3) mixture of step (1) gained is cooled to 35 DEG C, joins in the mixture that step (2) obtains, stir;
(4) mixture obtained to step (3) adds ultra-pure water, be 100 parts to mixture gross mass.
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| CN113041209A (en) * | 2021-04-12 | 2021-06-29 | 东晟源研究院(广州)有限公司 | Whitening mask cream |
| CN115215948A (en) * | 2022-06-02 | 2022-10-21 | 润辉生物技术（威海）有限公司 | Low-molecular hyaluronan decarboxylated carnosine derivative, and preparation method and application thereof |
| CN115215948B (en) * | 2022-06-02 | 2023-07-11 | 润辉生物技术（威海）有限公司 | Low-molecular hyaluronan decarboxylated carnosine derivative, preparation method and application |
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