CN105614750A - Making method of sea cucumber oral administration stock solution and sea cucumber buccal tablets - Google Patents
Making method of sea cucumber oral administration stock solution and sea cucumber buccal tablets Download PDFInfo
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- 241000251511 Holothuroidea Species 0.000 title claims abstract description 153
- 238000000034 method Methods 0.000 title claims abstract description 71
- 239000006189 buccal tablet Substances 0.000 title abstract description 5
- 239000011550 stock solution Substances 0.000 title abstract 3
- 239000007788 liquid Substances 0.000 claims abstract description 94
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 38
- 238000009835 boiling Methods 0.000 claims abstract description 34
- 239000000843 powder Substances 0.000 claims abstract description 32
- 238000010612 desalination reaction Methods 0.000 claims abstract description 28
- 239000000463 material Substances 0.000 claims abstract description 17
- 235000013305 food Nutrition 0.000 claims abstract description 15
- 238000004519 manufacturing process Methods 0.000 claims description 46
- 239000007937 lozenge Substances 0.000 claims description 26
- 241000965254 Apostichopus japonicus Species 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 19
- 235000013409 condiments Nutrition 0.000 claims description 13
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 11
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 229930195725 Mannitol Natural products 0.000 claims description 11
- 244000269722 Thea sinensis Species 0.000 claims description 11
- 235000009569 green tea Nutrition 0.000 claims description 11
- 235000010355 mannitol Nutrition 0.000 claims description 11
- 239000000594 mannitol Substances 0.000 claims description 11
- 230000007062 hydrolysis Effects 0.000 claims description 10
- 238000006460 hydrolysis reaction Methods 0.000 claims description 10
- 210000001835 viscera Anatomy 0.000 claims description 10
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- 238000010411 cooking Methods 0.000 claims description 9
- 238000011033 desalting Methods 0.000 claims description 9
- 238000001694 spray drying Methods 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 102000005158 Subtilisins Human genes 0.000 claims description 7
- 108010056079 Subtilisins Proteins 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 108090000623 proteins and genes Proteins 0.000 claims description 7
- 239000003826 tablet Substances 0.000 claims description 7
- 239000000758 substrate Substances 0.000 claims description 6
- 238000013019 agitation Methods 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000013467 fragmentation Methods 0.000 claims description 5
- 238000006062 fragmentation reaction Methods 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 238000005507 spraying Methods 0.000 claims description 5
- 239000008399 tap water Substances 0.000 claims description 5
- 235000020679 tap water Nutrition 0.000 claims description 5
- 238000005550 wet granulation Methods 0.000 claims description 5
- 235000020985 whole grains Nutrition 0.000 claims description 5
- 230000002378 acidificating effect Effects 0.000 claims description 4
- 239000000796 flavoring agent Substances 0.000 claims description 4
- 235000013355 food flavoring agent Nutrition 0.000 claims description 4
- 238000005342 ion exchange Methods 0.000 claims description 4
- 230000000844 anti-bacterial effect Effects 0.000 claims description 3
- 235000012907 honey Nutrition 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 230000002401 inhibitory effect Effects 0.000 abstract description 8
- 239000002994 raw material Substances 0.000 abstract description 6
- 150000004676 glycans Chemical class 0.000 abstract description 3
- 229920001282 polysaccharide Polymers 0.000 abstract description 3
- 239000005017 polysaccharide Substances 0.000 abstract description 3
- 102000008186 Collagen Human genes 0.000 abstract description 2
- 108010035532 Collagen Proteins 0.000 abstract description 2
- 239000000243 solution Substances 0.000 abstract 3
- 230000036772 blood pressure Effects 0.000 abstract 2
- 239000000413 hydrolysate Substances 0.000 abstract 2
- 239000000575 pesticide Substances 0.000 abstract 2
- 230000001603 reducing effect Effects 0.000 abstract 2
- 229920001436 collagen Polymers 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 235000014102 seafood Nutrition 0.000 abstract 1
- 102100030988 Angiotensin-converting enzyme Human genes 0.000 description 12
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 12
- 239000000523 sample Substances 0.000 description 6
- 230000031700 light absorption Effects 0.000 description 4
- AAXWBCKQYLBQKY-IRXDYDNUSA-N (2s)-2-[[(2s)-2-[(2-benzamidoacetyl)amino]-3-(1h-imidazol-5-yl)propanoyl]amino]-4-methylpentanoic acid Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(O)=O)NC(=O)CNC(=O)C=1C=CC=CC=1)C1=CN=CN1 AAXWBCKQYLBQKY-IRXDYDNUSA-N 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
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- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
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- 108010016268 hippuryl-histidyl-leucine Proteins 0.000 description 3
- 239000003112 inhibitor Substances 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- UUUHXMGGBIUAPW-UHFFFAOYSA-N 1-[1-[2-[[5-amino-2-[[1-[5-(diaminomethylideneamino)-2-[[1-[3-(1h-indol-3-yl)-2-[(5-oxopyrrolidine-2-carbonyl)amino]propanoyl]pyrrolidine-2-carbonyl]amino]pentanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]amino]-3-methylpentanoyl]pyrrolidine-2-carbon Chemical compound C1CCC(C(=O)N2C(CCC2)C(O)=O)N1C(=O)C(C(C)CC)NC(=O)C(CCC(N)=O)NC(=O)C1CCCN1C(=O)C(CCCN=C(N)N)NC(=O)C1CCCN1C(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)C1CCC(=O)N1 UUUHXMGGBIUAPW-UHFFFAOYSA-N 0.000 description 1
- BGNGWHSBYQYVRX-UHFFFAOYSA-N 4-(dimethylamino)benzaldehyde Chemical compound CN(C)C1=CC=C(C=O)C=C1 BGNGWHSBYQYVRX-UHFFFAOYSA-N 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 108010004032 Bromelains Proteins 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 108090000882 Peptidyl-Dipeptidase A Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 235000019835 bromelain Nutrition 0.000 description 1
- 229940046011 buccal tablet Drugs 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a making method of seafood health-care food with the blood pressure reducing function. Sea cucumbers serve as main raw materials, after the sea cucumber are boiled in water and subjected to desalination, and hydrolysate is obtained; boiled liquid obtained by boiling the sea cucumbers in boiling water is subjected to desalination, and desalinated boiled liquid is obtained; the hydrolysate and the desalinated boiled liquid are mixed into a sea cucumber extracting solution; the sea cucumber extracting solution is compounded with other auxiliary materials so that a sea cucumber oral administration stock solution and sea cucumber buccal tablets can be prepared. Compared with the prior art, original active components in the oral administration stock solution and the buccal tablets are reserved to the maximum extent, collagen low in nutritional value is converted into pesticide with blood pressure reducing activity by means of enzymolysis, the raw material utilizing rate is greatly increased, the ACE inhibiting rate of the prepared sea cucumber oral administration solution is 60-65%, the content of active pesticides is 900-1000 micrograms/mL, the polysaccharide content is 900-1000 micrograms/mL, and the prepared sea cucumber buccal tablets contain 18.0-21.0% sea cucumber powder.
Description
Technical field
The present invention relates to the preparation method of a kind of food, particularly the manufacture method of the oral stoste of a kind of sea cucumber. The present invention also relates to the manufacture method of a kind of sea cucumber lozenge.
Background technology
Sea cucumber is a kind of famous and precious nourishing food and medicinal material, the various active materials such as rich in proteins, mucopolysaccharide and selenka, and being preced with is first of " Hai Bazhen ". In recent years, the human consumer got more and more because of health care and the nutritive value of its uniqueness pays close attention to, and the consumer group constantly expands, and industry development is rapid.
In prior art, the open specification sheets CN1494914A of Chinese patent application discloses the production method of a kind of sea cucumber oral buccal tablet, it mainly sends out system, quick-frozen, vacuum hydro-extraction through cleaning sea cucumber, pulverize into powder, be processed into the step such as sheet, weather-proof dressing completes, but the method just whole sea cucumber is pulverized post-treatment to become tablet, it does not have sea cucumber is carried out deep processing process. The open specification sheets CN103610075A of Chinese patent application discloses the manufacture method of a kind of holothurian oral liquid, it is by sea cucumber lyophilize, through coarse reduction, micronizing and nanometer pulverizing, dry Stichopus japonicus is obtained Stichopus japonicus powder, Stichopus japonicus powder is dissolved in the water, add stomach en-and bromeline, it is hydrolyzed when microwave-assisted, separating liquid through the obtained sea cucumber extract of centrifugal, filter progress, sea cucumber extract coordinates with Chinese medicine extraction liquid, through the oral liquid that the operations such as filling, sterilization are made; The open specification sheets CN103610071A of Chinese patent application discloses the manufacture method of a kind of blood-pressure-reducsea sea cucumber oral liquid, sea cucumber lyophilize, dry Stichopus japonicus are pulverized through coarse reduction, micronizing and nanometer and are obtained Stichopus japonicus powder by the method, Stichopus japonicus powder is dissolved in the water, add stomach en-and bromeline, it is hydrolyzed when microwave-assisted, separate liquid through centrifugal, the obtained sea cucumber extract of filter progress, sea cucumber extract and Chinese medicine extract liquid and coordinate, and make the oral liquid with lipid-lowering effect through operations such as filling, sterilizations; Sea cucumber mainly pulverized by the manufacture method of above-mentioned 2 kinds of holothurian oral liquids, with stomach en-and bromelain hydrolyzate, the sea cucumber extract obtained again with other drug matching, make holothurian oral liquid, its weak point is, technique is comparatively complicated, and whether various effects that oral liquid has are still not clear by the offer of sea cucumber hydrolyzate.
Summary of the invention
Technical problem to be solved by this invention is for the deficiencies in the prior art, the manufacture method of the oral stoste of sea cucumber that a kind of technological design is reasonable, operation is more easy is provided, retain to greatest extent and make full use of the original activeconstituents of sea cucumber, it is to increase the utilization ratio of sea cucumber raw material.
Another technical problem to be solved by this invention is the manufacture method additionally providing a kind of sea cucumber lozenge.
Technical problem to be solved by this invention is realized by following technical scheme. The present invention is the manufacture method of the oral stoste of a kind of sea cucumber, is characterized in, its step is as follows:
(1) remove internal organ: cut off sea cucumber body cavity with scissors, remove the internal organ of sea cucumber, clean with tap water clean;
(2) boiling water boiling: cleaned sea cucumber is added water boil, agitation as appropriate in the process of boiling water boiling, sea cucumber is 1:3 ~ 4 with the mass ratio of the water added, and the water time of boiling is 20 ~ 30min, and after boiling, taking-up drains for subsequent use; Carry out desalting treatment to cooking liquid, obtain desalination and cook liquid;
(3) broken: in sea cucumber, to add water by solid liquid mass ratio 1:8 ~ 10, first with organizing crusher in crushing, and then make tissue fragmentation more abundant with colloidal mill; Obtained sea cucumber homogenate;
(4) enzymolysis: sea cucumber homogenate is warmed up to hydrolysis temperature in advance, adds Alcalase Sumizyme MP and is hydrolyzed, and boils 8 ~ 12min and carries out going out enzyme, and obtained hydrolyzed solution, is cooled to room temperature for subsequent use; Hydrolysis temperature 40 ~ 45 DEG C, pH=7.0 ~ 7.5, enzymolysis time 190 ~ 220min, enzyme concentration 40000 ~ 50000U/g substrate protein; Obtain hydrolyzed solution;
(5) allocate: hydrolyzed solution and desalination are cooked liquid and proportions, coordinate sea cucumber hydrolyzed solution in liquid to account for the 70 ~ 80% of total mass; Add suitable auxiliary and condiment again; Filtering, vacuum outgas, obtains original liquid of trepang food.
In the manufacture method of the oral stoste of a kind of sea cucumber of the present invention, preferred technical scheme or technology feature are further:
1., in step (2), liquid carries out desalting treatment to cooking to adopt ion exchange method, obtains desalination and cooks liquid.
2. in step (5), then the auxiliary and condiment added is acidic flavoring agent and/or sweeting agent.
3., in step (5), the auxiliary and condiment added is account for coordinate the citric acid of liquid mass percent 0.2% and the honey of 6.8%, and the pH of original liquid of trepang food is 4.01.
4. in step (5), the time of vacuum outgas is 40min, and temperature is 60 DEG C.
5. by original liquid of trepang food bottling obtained for step (5), sealing, sterilization, obtained holothurian oral liquid; Bactericidal formula in described sterilization process is: 10min-20min-10min/105 DEG C.
Technical problem to be solved by this invention can also be realized further by following technical scheme. Present invention also offers the manufacture method of a kind of sea cucumber lozenge, be characterized in, its step is as follows:
(1) sea cucumber hydrolyzed solution and desalination cook the preparation of liquid, with step (1) ~ (4) in the manufacture method of the above-mentioned oral stoste of a kind of sea cucumber;
(2) concentrating under reduced pressure: sea cucumber hydrolyzed solution and desalination are cooked liquid and proportions, coordinates sea cucumber hydrolyzed solution in liquid to account for the 70 ~ 80% of total mass; Cooperation liquid concentrating under reduced pressure is processed to the 1/2 ~ 1/3 of original volume, obtains concentrated solution;
(3) spraying dry: concentrated solution is carried out spray drying treatment, obtains Stichopus japonicus powder;
(4) mix: Stichopus japonicus powder is mixed with appropriate auxiliary material, obtains mixture;
(5) it is processed into sheet: the mixture that step (4) obtains is carried out wet granulation, drying, whole grain, total mixed, compressing tablet process, obtained sea cucumber lozenge.
In the manufacture method of a kind of sea cucumber lozenge of the present invention, described auxiliary material is any a kind of auxiliary material that can be used for making food tablet of the present invention disclosed in prior art or commercially available.
In the manufacture method of a kind of sea cucumber lozenge of the present invention, preferred technical scheme or technology feature are further:
1. in step (3), spray drying condition is: inlet temperature is 140 ~ 150 DEG C, and air outlet temperature is 80 ~ 85 DEG C, and mass flow is 800 ~ 900mL/h.
2. in step (4), described auxiliary material be selected from green tea powder, Walocel MT 20.000PV, W-Gum and N.F,USP MANNITOL one or more.
3. the auxiliary material described in is green tea powder, Walocel MT 20.000PV, W-Gum and N.F,USP MANNITOL, and the weight percent of mixture consists of: Stichopus japonicus powder 18.0 ~ 21.0%, green tea powder 3.0 ~ 6.0%, Walocel MT 20.000PV 4.0%, W-Gum 24.0%, N.F,USP MANNITOL 48.0%.
Compared with prior art, the present invention uses Alcalase enzymolysis after sea cucumber water is boiled desalination, obtaining hydrolyzed solution, described Alcalase is through what test of many times screened, and this enzyme can by the protein degradation in raw material for having the active small-molecular peptides of Angiotensin-converting enzyme inhibition (ACE); Adopt boiling water boiling sea cucumber, be conducive to extracting the water-soluble active ingredient in sea cucumber such as sea cucumber polysaccharide, selenka etc., namely obtain cooking liquid; Cook liquid through ion exchange method desalting treatment, reduce its salinity and reclaim its activeconstituents, namely obtain desalination and cook liquid; Hydrolyzed solution and desalination are cooked liquid and is mixed into sea cucumber extract, then make the oral stoste of sea cucumber and sea cucumber lozenge after composite to sea cucumber extract and other auxiliary materials. The inventive method remains the original activeconstituents of sea cucumber in oral stoste and lozenge to greatest extent, and by enzymolysis, collagen protein not high for nutritive value is also changed into the peptide class with hypotensive activity, the protein in raw material can be made full use of, greatly improve the utilization ratio of raw material. The ACE inhibiting rate of the holothurian oral liquid that the present invention obtains is 60 ~ 65%, and bioactive peptide content is 900 ~ 1000 �� g/mL, and polysaccharide content is 900 ~ 1000 �� g/mL; Containing the Stichopus japonicus powder of 18.0 ~ 21.0% in obtained sea cucumber lozenge.
Embodiment
Hereinafter further describe the concrete technical scheme of the present invention, so that the technician of this area understands the present invention further, and do not form the restriction to its right.
Embodiment 1, the manufacture method of the oral stoste of a kind of sea cucumber, its step is as follows:
(1) remove internal organ: cut off sea cucumber body cavity with scissors, remove the internal organ of sea cucumber, clean with tap water clean;
(2) boiling water boiling: cleaned sea cucumber is added water boil, agitation as appropriate in the process of boiling water boiling, sea cucumber is 1:3 with the mass ratio of the water added, and the water time of boiling is 20min, and after boiling, taking-up drains for subsequent use; Carry out desalting treatment to cooking liquid, obtain desalination and cook liquid;
(3) broken: in sea cucumber, to add water by solid liquid mass ratio 1:8, first with organizing crusher in crushing, and then make tissue fragmentation more abundant with colloidal mill; Obtained sea cucumber homogenate;
(4) enzymolysis: sea cucumber homogenate is warmed up to hydrolysis temperature in advance, adds Alcalase Sumizyme MP and is hydrolyzed, and boils 8min and carries out going out enzyme, and obtained hydrolyzed solution, is cooled to room temperature for subsequent use; Hydrolysis temperature 40 DEG C, pH=7.0, enzymolysis time 190min, enzyme concentration 40000U/g substrate protein; Obtain hydrolyzed solution;
(5) allocate: hydrolyzed solution and desalination are cooked liquid and proportions, coordinate sea cucumber hydrolyzed solution in liquid to account for the 70% of total mass; Add suitable auxiliary and condiment again; Filtering, vacuum outgas, obtains original liquid of trepang food.
Embodiment 2, the manufacture method of the oral stoste of a kind of sea cucumber, its step is as follows:
(1) remove internal organ: cut off sea cucumber body cavity with scissors, remove the internal organ of sea cucumber, clean with tap water clean;
(2) boiling water boiling: cleaned sea cucumber is added water boil, agitation as appropriate in the process of boiling water boiling, sea cucumber is 1:4 with the mass ratio of the water added, and the water time of boiling is 30min, and after boiling, taking-up drains for subsequent use; Carry out desalting treatment to cooking liquid, obtain desalination and cook liquid;
(3) broken: in sea cucumber, to add water by solid liquid mass ratio 1:10, first with organizing crusher in crushing, and then make tissue fragmentation more abundant with colloidal mill; Obtained sea cucumber homogenate;
(4) enzymolysis: sea cucumber homogenate is warmed up to hydrolysis temperature in advance, adds Alcalase Sumizyme MP and is hydrolyzed, and boils 12min and carries out going out enzyme, and obtained hydrolyzed solution, is cooled to room temperature for subsequent use; Hydrolysis temperature 45 DEG C, pH=7.5, enzymolysis time 220min, enzyme concentration 50000U/g substrate protein; Obtain hydrolyzed solution;
(5) allocate: hydrolyzed solution and desalination are cooked liquid and proportions, coordinate sea cucumber hydrolyzed solution in liquid to account for the 80% of total mass; Add suitable auxiliary and condiment again; Filtering, vacuum outgas, obtains original liquid of trepang food.
Embodiment 3, the manufacture method of the oral stoste of a kind of sea cucumber, its step is as follows:
(1) remove internal organ: cut off sea cucumber body cavity with scissors, remove the internal organ of sea cucumber, clean with tap water clean;
(2) boiling water boiling: cleaned sea cucumber is added water boil, agitation as appropriate in the process of boiling water boiling, sea cucumber is 1:4 with the mass ratio of the water added, and the water time of boiling is 25min, and after boiling, taking-up drains for subsequent use; Carry out desalting treatment to cooking liquid, obtain desalination and cook liquid;
(3) broken: in sea cucumber, to add water by solid liquid mass ratio 1:9, first with organizing crusher in crushing, and then make tissue fragmentation more abundant with colloidal mill; Obtained sea cucumber homogenate;
(4) enzymolysis: sea cucumber homogenate is warmed up to hydrolysis temperature in advance, adds Alcalase Sumizyme MP and is hydrolyzed, and boils 10min and carries out going out enzyme, and obtained hydrolyzed solution, is cooled to room temperature for subsequent use; Hydrolysis temperature 45 DEG C, pH=7.0, enzymolysis time 200min, enzyme concentration 45000U/g substrate protein; Obtain hydrolyzed solution;
(5) allocate: hydrolyzed solution and desalination are cooked liquid and proportions, coordinate sea cucumber hydrolyzed solution in liquid to account for the 75% of total mass; Add suitable auxiliary and condiment again; Filtering, vacuum outgas, obtains original liquid of trepang food.
Embodiment 4, in the step (2) of the manufacture method of the oral stoste of a kind of sea cucumber of embodiment 1-3 described in any one: liquid carries out desalting treatment to cooking to adopt ion exchange method, obtains desalination and cooks liquid.
Embodiment 5, in the step (5) of the manufacture method of the oral stoste of a kind of sea cucumber of embodiment 1-4 described in any one: the auxiliary and condiment added is acidic flavoring agent and sweeting agent.
Embodiment 6, in the step (5) of the manufacture method of the oral stoste of a kind of sea cucumber of embodiment 1-4 described in any one: the auxiliary and condiment added is acidic flavoring agent.
Embodiment 7, in the step (5) of the manufacture method of the oral stoste of a kind of sea cucumber of embodiment 1-4 described in any one: the auxiliary and condiment added is sweeting agent.
Embodiment 8, in the step (5) of the manufacture method of the oral stoste of a kind of sea cucumber of embodiment 1-5 described in any one: the auxiliary and condiment added is account for coordinate the citric acid of liquid mass percent 0.2% and the honey of 6.8%, and the pH of original liquid of trepang food is 4.01.
Embodiment 9, in the step (5) of the manufacture method of the oral stoste of a kind of sea cucumber of embodiment 1-8 described in any one: the time of vacuum outgas is 40min, and temperature is 60 DEG C.
Embodiment 10, in the manufacture method of the oral stoste of a kind of sea cucumber of embodiment 1-9 described in any one, by original liquid of trepang food bottling obtained for step (5), sealing, sterilization, obtained holothurian oral liquid; Bactericidal formula in described sterilization process is: 10min-20min-10min/105 DEG C.
Embodiment 11, the manufacture method of a kind of sea cucumber lozenge, its step is as follows:
(1) sea cucumber hydrolyzed solution and desalination cook the preparation of liquid, with step (1) ~ (4) in the manufacture method of the oral stoste of a kind of sea cucumber of embodiment 19 described in any one;
(2) concentrating under reduced pressure: sea cucumber hydrolyzed solution and desalination are cooked liquid and proportions, coordinates sea cucumber hydrolyzed solution in liquid to account for the 70% of total mass; Cooperation liquid concentrating under reduced pressure is processed to the 1/2 of original volume, obtains concentrated solution;
(3) spraying dry: concentrated solution is carried out spray drying treatment, obtains Stichopus japonicus powder;
(4) mix: Stichopus japonicus powder is mixed with appropriate auxiliary material, obtains mixture;
(5) it is processed into sheet: the mixture that step (4) obtains is carried out wet granulation, drying, whole grain, total mixed, compressing tablet process, obtained sea cucumber lozenge.
Embodiment 12, the manufacture method of a kind of sea cucumber lozenge, its step is as follows:
(1) sea cucumber hydrolyzed solution and desalination cook the preparation of liquid, with step (1) ~ (4) in the manufacture method of the oral stoste of a kind of sea cucumber of embodiment 19 described in any one;
(2) concentrating under reduced pressure: sea cucumber hydrolyzed solution and desalination are cooked liquid and proportions, coordinates sea cucumber hydrolyzed solution in liquid to account for the 80% of total mass; Cooperation liquid concentrating under reduced pressure is processed to the 1/3 of original volume, obtains concentrated solution;
(3) spraying dry: concentrated solution is carried out spray drying treatment, obtains Stichopus japonicus powder;
(4) mix: Stichopus japonicus powder is mixed with appropriate auxiliary material, obtains mixture;
(5) it is processed into sheet: the mixture that step (4) obtains is carried out wet granulation, drying, whole grain, total mixed, compressing tablet process, obtained sea cucumber lozenge.
Embodiment 13, the manufacture method of a kind of sea cucumber lozenge, its step is as follows:
(1) sea cucumber hydrolyzed solution and desalination cook the preparation of liquid, with step (1) ~ (4) in the manufacture method of the oral stoste of a kind of sea cucumber of embodiment 19 described in any one;
(2) concentrating under reduced pressure: sea cucumber hydrolyzed solution and desalination are cooked liquid and proportions, coordinates sea cucumber hydrolyzed solution in liquid to account for the 75% of total mass; Cooperation liquid concentrating under reduced pressure is processed to the 1/2 of original volume, obtains concentrated solution;
(3) spraying dry: concentrated solution is carried out spray drying treatment, obtains Stichopus japonicus powder;
(4) mix: Stichopus japonicus powder is mixed with appropriate auxiliary material, obtains mixture;
(5) it is processed into sheet: the mixture that step (4) obtains is carried out wet granulation, drying, whole grain, total mixed, compressing tablet process, obtained sea cucumber lozenge.
Embodiment 14, in the step (3) of the manufacture method of a kind of sea cucumber lozenge of embodiment 11-13 described in any one: spray drying condition is: inlet temperature is 140 DEG C, and air outlet temperature is 80 DEG C, and mass flow is 800mL/h.
Embodiment 15, in the step (3) of the manufacture method of a kind of sea cucumber lozenge of embodiment 11-13 described in any one: spray drying condition is: inlet temperature is 150 DEG C, and air outlet temperature is 85 DEG C, and mass flow is 900mL/h.
Embodiment 16, in the step (4) of the manufacture method of a kind of sea cucumber lozenge of embodiment 11-15 described in any one, described auxiliary material be selected from green tea powder, Walocel MT 20.000PV, W-Gum and N.F,USP MANNITOL one or more.
Embodiment 17, in the manufacture method of a kind of sea cucumber lozenge of embodiment 11-15 described in any one, described auxiliary material is green tea powder, Walocel MT 20.000PV, W-Gum and N.F,USP MANNITOL, and the weight percent of mixture consists of: Stichopus japonicus powder 21.0%, green tea powder 3.0%, Walocel MT 20.000PV 4.0%, W-Gum 24.0%, N.F,USP MANNITOL 48.0%.
Embodiment 18, in the manufacture method of a kind of sea cucumber lozenge of embodiment 11-15 described in any one, described auxiliary material is green tea powder, Walocel MT 20.000PV, W-Gum and N.F,USP MANNITOL, and the weight percent of mixture consists of: Stichopus japonicus powder 18.0%, green tea powder 6.0%, Walocel MT 20.000PV 4.0%, W-Gum 24.0%, N.F,USP MANNITOL 48.0%.
Embodiment 19, it may also be useful to the obtained holothurian oral liquid of embodiment 10 carries out ACE inhibitory activity determination experiment as sample, and recording its ACE inhibiting rate is 60 ~ 65%. The measuring method of ACE inhibitory activity is: DAB(3,3-tetra-diamine hydrochloride p-diaminodiphenyl) development process, concrete operation is as follows:
1. the preparation of reagent
(1) 1mol/LHCl gets the dense HCl of 8.3mL in the 100mL volumetric flask filling distilled water in advance, mixed even, is settled to scale.
(2) hippuryl histidyl-leucine (HHL) solution: 25mgHHL is dissolved in 7.02mL0.1mol/L borate buffer, is made into 8.3mmol/LHHL solution.
The DAB developer of (3) 0.5%: 0.5g paradimethy laminobenzaldehyde is dissolved in 100mL pyridine, mixed even, now with the current.
2. experimental procedure
Sample sets and blank group agents useful for same are as shown in table 1.
Table 1.ACE inhibit activities experimental establishment table.
(1) add the reactant of corresponding amount at each experiment tube by table 1, wherein substrate HHL, sample liquid (inhibitor), ACE need at 37 DEG C preheating 10min, each reactant needs to mix after adding;
(2) after adding, each pipe reacts the accurate timing of 60min(at 37 DEG C);
(3) after reaction terminates, each test tube adds the HCl termination reaction of 250 �� L1mol/L immediately, mixed even on whirlpool instrument;
(4) add 1.5mL ethyl acetate to extract, intense oscillations 1min on whirlpool instrument, at the centrifugal 5min of 3000r/min;
(5) 0.7mL upper strata ethyl acetate layer is got, the ethyl acetate of the group 0.7ml that directly adds in addition. Adding the DAB developer of 1ml diacetyl oxide and 2ml0.5% again, develop the color 30min in 40 DEG C of water-baths;
(6), after reaction terminates, its light absorption value is measured in 459nm place;
3.ACE inhibiting rate calculation formula is:
In formula: A-replaces, with water, the light absorption value that sample liquid (inhibitor) measures;
S-adds the light absorption value that sample liquid (inhibitor) measures;
The ginseng that C-adds ethyl acetate, diacetyl oxide and developer compares light absorption value.
4.IC50The mensuration of value
IC50The mass concentration (mg/mL) of holothurian oral liquid sample when the suppression being defined as ACE reaches 50%. Holothurian oral liquid diluted sample is become different multiples, then adopts its ACE inhibitory activity of DAB determination of color, make the dose-effect relationship figure of holothurian oral liquid mass concentration and ACE inhibiting rate, then adopt IC according to Logit model50Counter calculates its IC50Value.
Claims (10)
1. the manufacture method of the oral stoste of sea cucumber, it is characterised in that, its step is as follows:
(1) remove internal organ: cut off sea cucumber body cavity with scissors, remove the internal organ of sea cucumber, clean with tap water clean;
(2) boiling water boiling: cleaned sea cucumber is added water boil, agitation as appropriate in the process of boiling water boiling, sea cucumber is 1:3 ~ 4 with the mass ratio of the water added, and the water time of boiling is 20 ~ 30min, and after boiling, taking-up drains for subsequent use; Carry out desalting treatment to cooking liquid, obtain desalination and cook liquid;
(3) broken: in sea cucumber, to add water by solid liquid mass ratio 1:8 ~ 10, first with organizing crusher in crushing, and then make tissue fragmentation more abundant with colloidal mill; Obtained sea cucumber homogenate;
(4) enzymolysis: sea cucumber homogenate is warmed up to hydrolysis temperature in advance, adds Alcalase Sumizyme MP and is hydrolyzed, and boils 8 ~ 12min and carries out going out enzyme, and obtained hydrolyzed solution, is cooled to room temperature for subsequent use; Hydrolysis temperature 40 ~ 45 DEG C, pH=7.0 ~ 7.5, enzymolysis time 190 ~ 220min, enzyme concentration 40000 ~ 50000U/g substrate protein; Obtain hydrolyzed solution;
(5) allocate: hydrolyzed solution and desalination are cooked liquid and proportions, coordinate sea cucumber hydrolyzed solution in liquid to account for the 70 ~ 80% of total mass; Add suitable auxiliary and condiment again; Filtering, vacuum outgas, obtains original liquid of trepang food.
2. the manufacture method of the oral stoste of sea cucumber according to claim 1, it is characterised in that, in step (2), liquid carries out desalting treatment to cooking to adopt ion exchange method, obtains desalination and cooks liquid.
3. the manufacture method of the oral stoste of sea cucumber according to claim 1, it is characterised in that, in step (5), the auxiliary and condiment added is acidic flavoring agent and/or sweeting agent.
4. the manufacture method of the oral stoste of sea cucumber according to claim 1, it is characterised in that, in step (5), the auxiliary and condiment added is account for coordinate the citric acid of liquid mass percent 0.2% and the honey of 6.8%, and the pH of original liquid of trepang food is 4.01.
5. the manufacture method of the oral stoste of sea cucumber according to claim 1, it is characterised in that, in step (5), the time of vacuum outgas is 40min, and temperature is 60 DEG C.
6. the manufacture method of the oral stoste of sea cucumber according to claim 1, it is characterised in that, by original liquid of trepang food bottling obtained for step (5), sealing, sterilization, obtained holothurian oral liquid; Bactericidal formula in described sterilization process is: 10min-20min-10min/105 DEG C.
7. the manufacture method of a sea cucumber lozenge, it is characterised in that, its step is as follows:
(1) sea cucumber hydrolyzed solution and desalination cook the preparation of liquid, with step (1) ~ (4) of claim 1;
(2) concentrating under reduced pressure: sea cucumber hydrolyzed solution and desalination are cooked liquid and proportions, coordinates sea cucumber hydrolyzed solution in liquid to account for the 70 ~ 80% of total mass; Cooperation liquid concentrating under reduced pressure is processed to the 1/2 ~ 1/3 of original volume, obtains concentrated solution;
(3) spraying dry: concentrated solution is carried out spray drying treatment, obtains Stichopus japonicus powder;
(4) mix: Stichopus japonicus powder is mixed with appropriate auxiliary material, obtains mixture;
(5) it is processed into sheet: the mixture that step (4) obtains is carried out wet granulation, drying, whole grain, total mixed, compressing tablet process, obtained sea cucumber lozenge.
8. the manufacture method of a kind of sea cucumber lozenge according to claim 7, it is characterised in that, in step (3), spray drying condition is: inlet temperature is 140 ~ 150 DEG C, and air outlet temperature is 80 ~ 85 DEG C, and mass flow is 800 ~ 900mL/h.
9. the manufacture method of a kind of sea cucumber lozenge according to claim 7, it is characterised in that, in step (4), described auxiliary material be selected from green tea powder, Walocel MT 20.000PV, W-Gum and N.F,USP MANNITOL one or more.
10. the manufacture method of a kind of sea cucumber lozenge according to claim 9, it is characterized in that: described auxiliary material is green tea powder, Walocel MT 20.000PV, W-Gum and N.F,USP MANNITOL, and the weight percent of mixture consists of: Stichopus japonicus powder 18.0 ~ 21.0%, green tea powder 3.0 ~ 6.0%, Walocel MT 20.000PV 4.0%, W-Gum 24.0%, N.F,USP MANNITOL 48.0%.
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| CN106539075A (en) * | 2016-07-18 | 2017-03-29 | 陈伟宁 | A kind of preparation method of efficient absorption sea cucumber nutrient buccal tablet |
| CN106923240A (en) * | 2017-03-02 | 2017-07-07 | 烟台沃特福得水产科技有限公司 | A kind of preparation method of the oral stoste of petrel and petrel lozenge |
| CN107484984A (en) * | 2017-09-29 | 2017-12-19 | 孔令娟 | A kind of stichopus japonicus oral liquid and its manufacture method rich in sea cucumber active polysaccharide |
| CN107712692A (en) * | 2017-11-23 | 2018-02-23 | 黑龙江美丽石岛科技开发有限公司 | A kind of method that nutriment is extracted from unsalted dried sea cucumbers |
| CN108208631A (en) * | 2018-02-07 | 2018-06-29 | 青岛浩然生态海洋水产科技有限公司 | A kind of sea cucumber adds the production technology of calcium product |
| CN108308558A (en) * | 2018-02-07 | 2018-07-24 | 青岛浩然生态海洋水产科技有限公司 | A kind of production technology of instant holothurian |
| CN111213854A (en) * | 2020-02-27 | 2020-06-02 | 柳小露 | Production process of novel healthy puffed food |
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Application publication date: 20160601 |