CN105613383B - A kind of purple imitates the selection of stichopus japonicus - Google Patents
A kind of purple imitates the selection of stichopus japonicus Download PDFInfo
- Publication number
- CN105613383B CN105613383B CN201610067958.1A CN201610067958A CN105613383B CN 105613383 B CN105613383 B CN 105613383B CN 201610067958 A CN201610067958 A CN 201610067958A CN 105613383 B CN105613383 B CN 105613383B
- Authority
- CN
- China
- Prior art keywords
- purple
- stichopus japonicus
- selection
- feed
- pond
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000965254 Apostichopus japonicus Species 0.000 title claims abstract description 50
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 35
- 238000000034 method Methods 0.000 claims abstract description 33
- 210000001015 abdomen Anatomy 0.000 claims abstract description 20
- 238000009395 breeding Methods 0.000 claims abstract description 13
- 230000001488 breeding effect Effects 0.000 claims abstract description 13
- 238000004040 coloring Methods 0.000 claims abstract description 11
- 230000012447 hatching Effects 0.000 claims abstract description 7
- 230000008569 process Effects 0.000 claims abstract description 7
- 230000007850 degeneration Effects 0.000 claims abstract description 6
- 238000002955 isolation Methods 0.000 claims abstract description 5
- 230000000737 periodic effect Effects 0.000 claims abstract description 5
- 238000004321 preservation Methods 0.000 claims abstract description 5
- 210000003128 head Anatomy 0.000 claims description 20
- 241001237732 Microtia Species 0.000 claims description 12
- 210000004027 cell Anatomy 0.000 claims description 12
- 239000004568 cement Substances 0.000 claims description 12
- 230000037396 body weight Effects 0.000 claims description 11
- 238000012216 screening Methods 0.000 claims description 11
- 241000208340 Araliaceae Species 0.000 claims description 10
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 10
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 10
- 235000008434 ginseng Nutrition 0.000 claims description 10
- 241000195493 Cryptophyta Species 0.000 claims description 9
- 102000002322 Egg Proteins Human genes 0.000 claims description 8
- 108010000912 Egg Proteins Proteins 0.000 claims description 8
- 210000004681 ovum Anatomy 0.000 claims description 8
- 241001465805 Nymphalidae Species 0.000 claims description 7
- 210000000959 ear middle Anatomy 0.000 claims description 7
- 235000013601 eggs Nutrition 0.000 claims description 7
- 239000003337 fertilizer Substances 0.000 claims description 7
- 238000000926 separation method Methods 0.000 claims description 7
- 230000006641 stabilisation Effects 0.000 claims description 6
- 238000011105 stabilization Methods 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 claims description 5
- 230000000638 stimulation Effects 0.000 claims description 5
- 241000227752 Chaetoceros Species 0.000 claims description 4
- 244000017020 Ipomoea batatas Species 0.000 claims description 4
- 235000002678 Ipomoea batatas Nutrition 0.000 claims description 4
- 241000223252 Rhodotorula Species 0.000 claims description 4
- 230000004075 alteration Effects 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- 238000012505 colouration Methods 0.000 claims description 4
- 210000004907 gland Anatomy 0.000 claims description 4
- 230000035800 maturation Effects 0.000 claims description 4
- 238000000386 microscopy Methods 0.000 claims description 4
- 230000003278 mimic effect Effects 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- 239000003973 paint Substances 0.000 claims description 4
- 230000032696 parturition Effects 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 238000013139 quantization Methods 0.000 claims description 4
- 239000013535 sea water Substances 0.000 claims description 4
- 230000001568 sexual effect Effects 0.000 claims description 4
- 238000003860 storage Methods 0.000 claims description 4
- 230000001360 synchronised effect Effects 0.000 claims description 4
- 230000007704 transition Effects 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 3
- 241001632422 Radiola linoides Species 0.000 claims 1
- 239000000203 mixture Substances 0.000 abstract description 2
- 239000000049 pigment Substances 0.000 abstract 1
- 239000003086 colorant Substances 0.000 description 5
- 241001632427 Radiola Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 3
- 238000013475 authorization Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 235000005686 eating Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000009313 farming Methods 0.000 description 2
- 238000012214 genetic breeding Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000001149 cognitive effect Effects 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000006694 eating habits Nutrition 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 238000009364 mariculture Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000002020 sage Nutrition 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention provides the selection that a kind of purple imitates stichopus japonicus, is characterized in：The back of the body, the pure wild imitative stichopus japonicus of abdomen entire body purple are collected, temporary support of independent isolation in cultivating pool is moved to and preserves；Filter out seed selection basic population mid-May, carry out artificial induced spawning, hatching and seed selection；When there is 30% floating larva to be developed to doliolaria, attachment base is launched, water is changed, launches feed；Its body colour of periodic logging and situation of growing, and screen the individual for eliminating difference；Cultivate overall process to carry out in purple environment, and be aided with and feed the feed enhancement seedling coloring containing purple natural pigment composition；The F1 generation ZICISHEN of seed selection is selfed and hybridized with the basic population of preservation respectively as parent, forms F2 filial generations, breeding technique technique and the same F1 generation of breeding condition of F2 filial generations.By through 3 high intensity standard seed selections, obtaining F3 for purple stichopus japonicus new lines, rate of selecting and remain is more than 90%, and the speed of growth and degeneration-resistant character and original seed stichopus japonicus are without significant difference.
Description
Technical field
The present invention relates to the selection of mariculture technology, the specifically a kind of imitative stichopus japonicus of purple.
Background technology
Since two thousand three, being continuously increased with consumption demand, China imitate apostichopus japonicus culture scale and rapidly expanded, forms
" the 5th time " sea-farming industry tide after algae, shellfish, shrimp, fish, cultivate output value highest as China's single variety
Sea-farming leading industry, existing imitative about 214945 hectares of the apostichopus japonicus culture area in the whole nation, 193705 tons of annual production, annual value of production reaches
More than 35000000000 yuan.
Imitative stichopus japonicus is also known as the stichopus japonicus in grey stichopus japonicus, ash ginseng, extra large mouse, that is, people's common saying, and its body colour is with growing environment
Change and change, more in yellow, brown, cyan, also white, the stichopus japonicus of entire body purple are extremely rare once in a while.
At present, in terms of imitative stichopus japonicus genetic breeding, only China is combined with selection and use with molecular biology in the world
Method carried out research, the objective trait of seed selection is mainly that the growth such as fast-growth, resistance to less salt, high temperature resistant, disease-resistant is related
Quantitative character, there is not breakthrough always for the seed selection of the qualitative characters such as apparent and body colour.In recent years, excavate apparent only
The new lines special, character is stable are as one of the important research direction in aquatic biological genetic breeding field.
China is the country for eating imitative stichopus japonicus earliest and at most in the world, its unique health care and the immune work of enhancing
With just enjoying people to favor since ancient times, be referred to as first of " marine products eight delicacies ", have in traditional eight big cuisines using imitative stichopus japonicus as
The advanced famous dish of main food materials, vegetable delicious and fragrant taste, mouthfeel are soft, have fully demonstrated in China's cooking culture " fragrance meaning shape is supported "
Top 5 factor, but in terms of " color " word key element that vegetable is judged, but due to imitating the different and not reasonable always of stichopus japonicus body colour
That thinks sentences opinion.Further, since consumption threshold is reduced in recent years and the change of people's eating habit, fresh jelly, instant, lyophilized etc. are protected
The converted products of stichopus japonicus primary colors is stayed to be increasingly becoming main flow, culinary art and edible way are also more with Baoshang（Congee）And dip in food, mix food etc.
Based on shortcut, this also proposes higher requirement to the apparent body colour for imitating stichopus japonicus.
China is the country for eating imitative stichopus japonicus earliest and at most in the world, and in China's tradition, purple is honorable face
Color, the gas of sage, emperor are represented, while be also splendid stimulation color.Therefore, the ZICISHEN of bright is relative to traditional
Grey, blue or green, brown or even white stichopus japonicus will have more preferable condition and orectic effect, and its market value must be far above existing product
Kind, Developing Prospects on Industrialized Exploitation is very wide.
How by the seed selection of high intensity, constantly collect, seed selection and growth fraction compared with etc. investigative test analyze, create system
Unite and perfect purple stichopus japonicus breeding technique route and technique, obtain Purpled traits genetic stability up to more than 90% new lines
Colony.This is technical problem urgently to be resolved hurrily at present.
The content of the invention
The present invention is to solve the problems, such as that prior art is present, there is provided a kind of purple imitates the selection of stichopus japonicus, by high-strength
Spend seed selection and obtain new lines colony of the Purpled traits genetic stability up to more than 90%, and stabilization characteristics of genetics, reach China in advance
《Aquatic products is former, breeding authorization procedure》Approving standard, purple imitate stichopus japonicus commercial value will be far above common variety.
The purpose of the present invention is achieved by the following technical solution：A kind of purple imitates the selection of stichopus japonicus, its feature
It is, the selection comprises the following steps：
A kind of purple imitates the selection of stichopus japonicus, it is characterised in that the selection comprises the following steps：
（1）The wild imitative stichopus japonicus of pure purples is collected extensively as seed selection basic population from natural marine site, moves to culturing pool
In individually isolation temporarily bring up fertilizer, storage pond, holding pond water keep pH value 7.8~8.5, more than dissolved oxygen 5mg/L, within 16 DEG C of temperature, salinity
30~35, temporarily feed mixed feed by the amount of stichopus japonicus body weight 1%~3% daily foster early stage, stop within 4~6 days to before hastening parturition；
（2）Annual the first tenday period of a month in May or the middle ten days, screen growth of more than individual weight 250g/ heads normally ingesting, energetic
Body is as fertilizer is temporarily brought up in seed selection basic population cement pit disposed within, using+the side of stimulation by running water of drying in the shade after sexual gland maturation
Method carries out artificial induced spawning, 19~21 DEG C of water temperature of laying eggs, and is taken out when having 3~5 sperms around each ovum of microscopy and by hero ginseng,
Adopt after ovum density reaches 7~10/mL and take out all seed ginsengs, and pond water temperature is risen to 22~23 DEG C and hatched, hatch
Period keeps micro- inflation, embryonated egg is evenly distributed；
（3）Hatching is arranged into water to selecting method to carry out seed selection with dragging during the microtia young by 0.24~0.26/mL density
Cultivated in the cement pit of 22~24 DEG C of temperature, the mimic colouration principle changed according to stichopus japonicus body colour, the wall of cement pit four brushes purple paint with bottom of pond
To build purple environment, feed based on Chaetoceros, salt algae, collocation feeds the alternative bait of ocean rhodotorula, and feeding volume is microtia
The cell/mL of the young 10,000, the cell/mL of the middle ear young 20,000, the big cell/mL of the ear young 30,000, quantity of exchanged water be the microtia young 1/4,
The middle ear young 1/3, the big ear young 1/2；
（4）When at least 30% floating larva is developed to doliolaria, the purple ripple sheet frame disinfected is launched
Do attachment base, attachment base is laid in bottom of pond, on place the attachment base of 1/2 quantity again and be uniformly distributed；Cultivate pond water temperature drop
It is extremely synchronous with nature seawater temperature, water is at least changed daily 1 time, each quantity of exchanged water is 45%~55% total range, launches attachment base
Afterwards 25~27 days for the first time fall pond, every 5~7 days afterwards fall pond once；Feed that progressively transition is by Micro Algae during floating larva
Artifical compound feed, 15%~20% purple sweet potato powder is added in mixed feed to strengthen seedling coloring, the total feeding volume of feed is seedling body
The 1%~3% of weight, and suitably adjusted according to the situation of ingesting；
（5）Seedling is cultivated and cultivation overall process is carried out in purple environment, its coloring of periodic logging and feelings of growing
Condition, and quantization screening is carried out using color difference meter, eliminate and body colour separation, slow-growing, morbidity, the individual of state difference occurs；Finally
Obtain F1 generation seed selection ZICISHEN；
（6）The F1 generation ZICISHEN of seed selection is reached maturity after cultivation in 2 years, is selfed respectively as parent and the base with preservation
Plinth colony hybridization, form the F2 filial generations of ZICISHEN, breeding technique technique and the same F1 generation of breeding condition of F2 filial generations；
（7）By continuous 3 generation high intensity standard seed selection, the F3 of stabilization characteristics of genetics is obtained for pure purples stichopus japonicus new product
System, rate is selected and remain up to more than 90%, and the speed of growth and degeneration-resistant character and original seed stichopus japonicus are without significant difference.
Improvement to above-mentioned technical proposal：The step（5）In, carry out quantifying screening generation body colour separation using color difference meter
Individual when, specific screening 4 times：
Carried out for the first time in body weight 2~3g/ heads, back L=25.4, a=5.9, b=1.4, belly colour index is：
L=30.9, a=6.1, b=2.1, rate are selected and remain as 30%；
Carried out for the second time in 45~55g/ heads, back colour index is：L=23.5, a=6.1, b=1.4, belly color refer to
It is designated as：
L=28.6, a=5.8, b=1.6, rate are selected and remain as 30%；
Third time is carried out in 90~110g/ heads, and back colour index is：L=21.2, a=6.2, b=1.3, belly color
Index is：
L=28.3, a=5.4, b=1.5, rate are selected and remain as 50%；
4th time it is long to 190~210g/ heads when carry out, back colour index is：L=19.0, a=6.4, b=1.5, belly
Colour index is：L=27.8,a=4.9,b=1.）, the rate of selecting and remain is at least 90%；
It is qualified to be judged with aberration △ E values≤5.
The present invention has many advantages and good effect compared with prior art：
1st, selection of the present invention is using purple body colour as objective trait, introduces biological mimic colouration principle and colorimeter measurement side
Method is quantified and screened to it, while takes into account the other quantity character advantage such as growth, degeneration-resistant, and seed selection purpose is strong, efficiency high
And be easy to standardization, large-scale promotion, usual seed selection to F3 be can reach more than 90% rate of selecting and remain, stabilization characteristics of genetics,
Reach China in advance《Aquatic products is former, breeding authorization procedure》Approving standard；
2nd, it is high, bright in luster beautiful by the ZICISHEN body colour purity of the inventive method seed selection, people can be met to new simultaneously
Strange affair thing and the consumer psychology demand of honorable experience, in addition people to imitate stichopus japonicus uniqueness nutrition health-care functions traditional cognitive,
The commercial value of product will be far above common variety, with the continuous expansion of industrialized scale, it is contemplated that unit price can be improved at least
More than 500%~50%, so as to which industrial economy benefit, increase fisherfolk income be substantially improved.
Embodiment
The present invention is described in further detail with reference to specific embodiment：
A kind of purple of the present invention imitates the embodiment of the selection of stichopus japonicus, and the selection comprises the following steps：
A kind of purple imitates the selection of stichopus japonicus, it is characterised in that the selection comprises the following steps：
（1）The wild imitative stichopus japonicus of pure purples is collected extensively as seed selection basic population from natural marine site, moves to culturing pool
In individually isolation temporarily bring up fertilizer, storage pond, holding pond water keep pH value 7.8~8.5, more than dissolved oxygen 5mg/L, within 16 DEG C of temperature, salinity
30~35, temporarily feed mixed feed by the amount of stichopus japonicus body weight 1%~3% daily foster early stage, stop within 4~6 days to before hastening parturition；
（2）Annual the first tenday period of a month in May or the middle ten days, screen growth of more than individual weight 250g/ heads normally ingesting, energetic
Body is as fertilizer is temporarily brought up in seed selection basic population cement pit disposed within, using+the side of stimulation by running water of drying in the shade after sexual gland maturation
Method carries out artificial induced spawning, 19~21 DEG C of water temperature of laying eggs, and is taken out when having 3~5 sperms around each ovum of microscopy and by hero ginseng,
Adopt after ovum density reaches 7~10/mL and take out all seed ginsengs, and pond water temperature is risen to 22~23 DEG C and hatched, hatch
Period keeps micro- inflation, embryonated egg is evenly distributed；
（3）Hatching is arranged into water to selecting method to carry out seed selection with dragging during the microtia young by 0.24~0.26/mL density
Cultivated in the cement pit of 22~24 DEG C of temperature, the mimic colouration principle changed according to stichopus japonicus body colour, the wall of cement pit four brushes purple paint with bottom of pond
To build purple environment, feed based on Chaetoceros, salt algae, collocation feeds the alternative bait of ocean rhodotorula, and feeding volume is microtia
The cell/mL of the young 10,000, the cell/mL of the middle ear young 20,000, the big cell/ml of the ear young 30,000, quantity of exchanged water be the microtia young 1/4, in
The ear young 1/3, the big ear young 1/2；
（4）When at least 30% floating larva is developed to doliolaria, the purple ripple sheet frame disinfected is launched
Do attachment base, attachment base is laid in bottom of pond, on place the attachment base of 1/2 quantity again and be uniformly distributed；Cultivate pond water temperature drop
It is extremely synchronous with nature seawater temperature, water is at least changed daily 1 time, each quantity of exchanged water is 45%~55% total range, launches attachment base
Afterwards 25~27 days for the first time fall pond, every 5~7 days afterwards fall pond once；Feed that progressively transition is by Micro Algae during floating larva
Artifical compound feed, 15%~20% purple sweet potato powder is added in mixed feed to strengthen seedling coloring, the total feeding volume of feed is seedling body
The 1%~3% of weight, and suitably adjusted according to the situation of ingesting；
（5）Seedling is cultivated and cultivation overall process is carried out in purple environment, its coloring of periodic logging and feelings of growing
Condition, and quantization screening is carried out using color difference meter, eliminate and body colour separation, slow-growing, morbidity, the individual of state difference occurs；Finally
Obtain F1 generation seed selection ZICISHEN；
（6）The F1 generation ZICISHEN of seed selection is reached maturity after cultivation in 2 years, is selfed respectively as parent and the base with preservation
Plinth colony hybridization, form the F2 filial generations of ZICISHEN, breeding technique technique and the same F1 generation of breeding condition of F2 filial generations；
（7）By continuous 3 generation high intensity standard seed selection, the F3 of stabilization characteristics of genetics is obtained for pure purples stichopus japonicus new product
System, rate is selected and remain up to more than 90%, and the speed of growth and degeneration-resistant character and original seed stichopus japonicus are without significant difference.
Above-mentioned steps（5）In, the specific method for using color difference meter quantify screening generation body colour separation individual is：Specifically
Screening 4 times, is carried out, back L=25.4, a=5.9, b=1.4, belly colour index is in body weight 2~3g/ heads for the first time：L=
30.9, a=6.1, b=2.1, rate is selected and remain as 30%；Carried out for the second time in 45~55g/ heads, back colour index is：L=23.5,a
=6.1, b=1.4, belly colour index are：L=28.6, a=5.8, b=1.6, rate are selected and remain as 30%；Third time is in 90~110g/ heads
Shi Jinhang, back colour index are：L=21.2, a=6.2, b=1.3, belly colour index are：L=28.3, a=5.4, b=1.5, choosing
It is 50% to stay rate；4th time it is long to 190~210g/ heads when carry out, back colour index is：
L=19.0, a=6.4, b=1.5, belly colour index are：L=27.8,a=4.9,b=1.）, the rate of selecting and remain is at least 90%；
It is qualified to be judged with aberration △ E values≤5.
Purple of the present invention imitates the specific embodiment of the selection of stichopus japonicus：
（1）From 2008, the pure wild imitative thorn of the back of the body, abdomen entire body purple is collected on Shandong Yantai, Weihai, Qingdao and other places respectively
Ginseng individual, moves in cultivating pool individually isolation and temporarily supports and preserve, storage pond, holding pond water keep pH value 7.8~8.5, dissolved oxygen 5mg/L with
Within upper, 16 DEG C of temperature, salinity 33, temporarily mixed feed is fed by the amount of stichopus japonicus body weight 1%~3% daily foster early stage, to before hastening parturition 5
It stops.
（2）May 12, growth of more than individual weight 250g/ heads normally ingesting, energetic individual are screened as seed selection basis
Fertilizer is temporarily brought up in colony's cement pit disposed within, after sexual gland maturation using dry in the shade+method of stimulation by running water carries out artificial induced spawning,
20 DEG C of water temperature of spawning, taken out when there are 3~5 sperms around each ovum of microscopy and by hero ginseng, adopt ovum density and reach 7~10
All seed ginsengs are taken out after individual/mL, and pond water temperature is risen to 22~23 DEG C and hatched, keep micro- inflation to make during hatching
Embryonated egg is evenly distributed.
（3）Hatching is arranged into 23 DEG C of temperature to selecting method to carry out seed selection with dragging during the microtia young by 0.25/mL density
Cement pit in cultivate, the wall of cement pit four and bottom of pond brush purple paint to build purple environment, feed based on Chaetoceros, salt algae,
Collocation feeds the alternative bait such as ocean rhodotorula, feeding volume is the cell/mL of the microtia young 10,000, the cell/mL of the middle ear young 20,000,
The big cell/mL of the ear young 30,000, quantity of exchanged water is the microtia young 1/4, the middle ear young 1/3, the big ear young 1/2.
（4）When there is 30% floating larva to be developed to doliolaria, launch the purple ripple sheet frame disinfected do it is attached
Base, attachment base is laid in bottom of pond, on place the attachment base of 1/2 quantity again and be uniformly distributed.Cultivate pond water temperature be down to
Nature seawater temperature is synchronous, changes water daily 1 time, every time 1/2 range, launches and falls within 25~27 days pond after attachment base for the first time, afterwards
Pond fall within every 5~7 days once.Feed that progressively transition is artifical compound feed by Micro Algae during floating larva, add in mixed feed
Add 15%~20% purple sweet potato powder to strengthen seedling coloring, the total feeding volume of feed is fitted for 1%~3% and the basis situation of ingesting of seedling body weight
Work as adjustment.
（5）Seedling is cultivated and cultivation overall process is carried out in purple environment, its coloring of periodic logging and feelings of growing
Condition, and quantization screening is carried out using color difference meter, eliminate and body colour separation, slow-growing, morbidity, the individual of state difference occurs, specifically
Screening 4 times：
Carried out for the first time in body weight 2~3g/ heads, colour index is：Back（L=25.4,a=5.9,b=1.4）,
Belly（L=30.9,a=6.1,b=2.1）, rate is selected and remain as 30%；
Carried out for the second time in 45~55g/ heads, colour index is：Back（L=23.5,a=6.1,b=1.4）,
Belly（L=28.6,a=5.8,b=1.6）, rate is selected and remain as 30%；
Third time is carried out in 90~110g/ heads, and colour index is：Back（L=21.2,a=6.2,b=1.3）,
Belly（L=28.3,a=5.4,b=1.5）, rate is selected and remain as 50%；
4th time it is long to 190~210g/ heads when carry out, colour index is：Back（L=19.0,a=6.4,b=1.5）,
Belly（L=27.8,a=4.9,b=1.3）, rate is selected and remain as 90%；
It is qualified to be judged with aberration △ E values≤5, final to obtain F1 generation seed selection ZICISHEN.
（6）The F1 generation ZICISHEN of seed selection is reached maturity after cultivation in 2 years, is selfed respectively as parent and the base with preservation
Plinth colony hybridization, form the F2 filial generations of ZICISHEN, breeding technique technique and the same F1 generation of breeding condition of F2 filial generations.
（7）By continuous 3 generation high intensity standard seed selection, the F3 of stabilization characteristics of genetics has been obtained at present for pure purples stichopus japonicus
New lines, rate is selected and remain up to 92.7%, and the speed of growth and degeneration-resistant character and original seed stichopus japonicus are without significant difference.ZICISHEN seed colours
Process data is shown in Table 1.
The ZICISHEN seed coloring process of table 1
| Tinting stage | Body grows (cm) | Body weight (g) |
| It is colourless | 0~0.12 | 0~0.007 |
| Back starts to colour | 0.12~0.15 | 0.007~0.0085 |
| Back colours successively | 0.15~0.5 | 0.0075~0.015 |
| Belly starts to colour | 0.4~0.5 | 0.0125~0.015 |
| Belly colours successively | 0.5~1.2 | 0.015~0.045 |
| Basic coloring, enteron aisle are visible | 1.2~2.5 | 0.045~0.35 |
| Body surface colours completely | 2.5~3.4 | 0.35~1.5 |
Certainly, described above is not limitation of the present invention, and the present invention is also not limited to the example above, this technology neck
The those of ordinary skill in domain, in the essential scope of the present invention, the variations, modifications, additions or substitutions made should all belong to this hair
Bright protection domain.
Claims (2)
1. a kind of purple imitates the selection of stichopus japonicus, it is characterised in that the selection comprises the following steps：
（1）The wild imitative stichopus japonicus of pure purples is collected extensively as seed selection basic population from natural marine site, is moved to single in culturing pool
Fertilizer is temporarily brought up in solely isolation, storage pond, holding pond water keep pH value 7.8~8.5, more than dissolved oxygen 5mg/L, within 16 DEG C of temperature, salinity 30~
35, temporarily feed mixed feed by the amount of stichopus japonicus body weight 1%~3% daily foster early stage, stop within 4~6 days to before hastening parturition；
（2）Annual the first tenday period of a month in May or the middle ten days, screen growth of more than individual weight 250g/ heads normally ingesting, energetic individual work
Temporarily to bring up fertilizer in seed selection basic population cement pit disposed within, after sexual gland maturation using dry in the shade+method of stimulation by running water enters
Row artificial induced spawning, 19~21 DEG C of water temperature of laying eggs, hero ginseng is taken out when there are 3~5 sperms around each ovum of microscopy, it is close to adopt ovum
Degree takes out all seed ginsengs after reaching 7~10/mL, and pond water temperature is risen into 22~23 DEG C and hatched, and is protected during hatching
Micro- inflation is held, embryonated egg is evenly distributed；
（3）Hatching to selecting method to carry out seed selection with dragging during the microtia young, by 0.24~0.26/ML density be arranged into water temperature 22~
Cultivated in 24 DEG C of cement pit, the mimic colouration principle changed according to stichopus japonicus body colour, the wall of cement pit four brushes purple paint with bottom of pond to build
Purple environment, feed based on Chaetoceros, salt algae, collocation feeds the alternative bait of ocean rhodotorula, and feeding volume is the microtia young 10,000
Cell/mL, the cell/mL of the middle ear young 20,000, the big cell/mL of the ear young 30,000, quantity of exchanged water are the microtia young 1/4, the middle ear young
1/ 3, the big ear young 1/2；
（4）When at least 30% floating larva is developed to doliolaria, launch the purple ripple sheet frame disinfected do it is attached
Base, attachment base is laid in bottom of pond, on place the attachment base of 1/2 quantity again and be uniformly distributed；Cultivate pond water temperature be down to
Nature seawater temperature is synchronous, at least changes water daily 1 time, and each quantity of exchanged water is 45%~55% total range, is launched 25 after attachment base
~27 days for the first time fall pond, every 5~7 days afterwards fall pond once；Feed that progressively transition is artificial by Micro Algae during floating larva
Mixed feed, 15%~20% purple sweet potato powder is added in mixed feed to strengthen seedling coloring, the total feeding volume of feed is seedling body weight
1%~3%, and suitably adjusted according to the situation of ingesting；
（5）Seedling is cultivated and cultivation overall process is carried out in purple environment, its coloring of periodic logging and situation of growing, and
Quantization screening is carried out using color difference meter, eliminates and body colour separation, slow-growing, morbidity, the individual of state difference occurs；It is final to obtain F1
For seed selection ZICISHEN；
（6）The F1 generation ZICISHEN of seed selection is reached maturity after cultivation in 2 years, is selfed respectively as parent and the underlying group with preservation
Body hybridizes, and forms the F2 filial generations of ZICISHEN, breeding technique technique and the same F1 generation of breeding condition of F2 filial generations；
（7）By continuous 3 generation high intensity standard seed selection, the F3 of stabilization characteristics of genetics is obtained for pure purples stichopus japonicus new lines, choosing
Rate is stayed up to more than 90%, and the speed of growth and degeneration-resistant character and original seed stichopus japonicus are without significant difference.
2. the selection of stichopus japonicus is imitated according to the purple described in claim 1, it is characterised in that the step（5）In, use color
When poor instrument quantify the individual of screening generation body colour separation, specifically screening 4 times：
Carried out for the first time in body weight 2~3g/ heads, back L=25.4, a=5.9, b=1.4, belly colour index is：
L=30.9, a=6.1, b=2.1, rate are selected and remain as 30%；
Carried out for the second time in 45~55g/ heads, back colour index is：L=23.5, a=6.1, b=1.4, belly colour index
For：
L=28.6, a=5.8, b=1.6, rate are selected and remain as 30%；
Third time is carried out in 90~110g/ heads, and back colour index is：L=21.2, a=6.2, b=1.3, belly colour index
For：
L=28.3, a=5.4, b=1.5, rate are selected and remain as 50%；
4th time it is long to 190~210g/ heads when carry out, back colour index is：L=19.0, a=6.4, b=1.5, belly color
Index is：L=27.8, a=4.9, b=1.3, the rate of selecting and remain are at least 90%；
It is qualified to be judged with aberration △ E values≤5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610067958.1A CN105613383B (en) | 2016-02-01 | 2016-02-01 | A kind of purple imitates the selection of stichopus japonicus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610067958.1A CN105613383B (en) | 2016-02-01 | 2016-02-01 | A kind of purple imitates the selection of stichopus japonicus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105613383A CN105613383A (en) | 2016-06-01 |
| CN105613383B true CN105613383B (en) | 2018-01-30 |
Family
ID=56029096
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610067958.1A Active CN105613383B (en) | 2016-02-01 | 2016-02-01 | A kind of purple imitates the selection of stichopus japonicus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105613383B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109744171A (en) * | 2019-03-04 | 2019-05-14 | 山东省海洋生物研究院 | A method of promoting spurs development and coloring |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110063280A (en) * | 2018-01-22 | 2019-07-30 | 大连鑫玉龙海洋生物种业科技股份有限公司 | Xia Fangfa is got in a kind of imitative stichopus japonicus kind interior |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2174749C1 (en) * | 2000-05-19 | 2001-10-20 | Государственное унитарное предприятие Тихоокеанский научно-исследовательский рыбохозяйственный центр | Trepang reproduction method |
| CN1720790B (en) * | 2004-07-14 | 2010-04-28 | 中国科学院海洋研究所 | An ecology breeding method for shallow sea seashell |
| CN101151964A (en) * | 2006-09-30 | 2008-04-02 | 山东好当家海洋发展股份有限公司 | Ecological cultivation method of biological bacterium sea cucumber |
| KR100915037B1 (en) * | 2007-05-15 | 2009-09-02 | 영어조합법인보전수산 | the combinated farming method and apparatus of the fish, the trepang |
| CN101322479B (en) * | 2008-07-17 | 2013-04-17 | 山东省海水养殖研究所 | Trepang intermediate nursing method |
| CN101606498A (en) * | 2009-07-06 | 2009-12-23 | 山东好当家海洋发展股份有限公司 | A kind of hybrid breeding method for stichopus japonicus |
-
2016
- 2016-02-01 CN CN201610067958.1A patent/CN105613383B/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109744171A (en) * | 2019-03-04 | 2019-05-14 | 山东省海洋生物研究院 | A method of promoting spurs development and coloring |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105613383A (en) | 2016-06-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101077063B (en) | Method for breeding triploid monomer oyster in scale | |
| CN105360035B (en) | The mating system of fancy carp | |
| WO2015021785A1 (en) | Method for breeding new-variety crassostrea gigas with orange left and right shells | |
| CN108308076A (en) | A kind of Chlamys nobilis " Nan'ao gold shellfish " new culturing technique | |
| CN106577415B (en) | A kind of producing method for seed of the long oyster new lines of black shell | |
| CN103858745B (en) | Tawny daylily algae artificial seedling rearing technology | |
| CN105613383B (en) | A kind of purple imitates the selection of stichopus japonicus | |
| CN109105338A (en) | A kind of producing method for seed of red plumage white shell egg chicken breed system | |
| CN104605171B (en) | A kind of method that manually alternative bait carries out bay scallop incubation | |
| CN109392782A (en) | One kind is like catfish Triplophysa industrialized cultivation method | |
| CN104705234A (en) | Artificial breeding method of botia superciliaris | |
| CN104663549B (en) | A kind of Marsupenaeus japonicus family feed coefficient marks the method slightly cultivated with pond | |
| CN104957055B (en) | A kind of selection of low-salt resistant juvenile stichopus | |
| CN108012964A (en) | A kind of breeding method of the long oyster new lines of cup type | |
| CN103461224B (en) | The system of selection of seed ginseng in stichopus japonicus crossbreeding | |
| CN105409884B (en) | A method of yellow plumage Gallus domesticlus brisson is cultivated in hybridization | |
| CN102100194A (en) | Fry rearing method for sinonovacula constricta | |
| CN107295997A (en) | A kind of method of construction of biological feed high density mixed breed culturing pool | |
| CN105706969B (en) | A kind of pure white imitates the selection of stichopus japonicus | |
| CN106135079A (en) | A kind of method of cultchless oyster pond intermediate rearing | |
| CN110012852A (en) | A kind of method for improving of offshore floating cage cultured large yellow croaker adult fish quality | |
| CN103999816A (en) | Method for value-added raising of high-quality free-range chickens | |
| KR100692378B1 (en) | Tintinnid, Undella sp. Chuuk-04 Available For Hatched Fry Feed And Culturing Method Thereof | |
| CN106538436A (en) | A kind of adhesive substrate cultivated suitable for cultchless oyster Seedling and preparation method thereof | |
| CN105660494A (en) | Shallow sea cage apostichopus japonicus recirculating aquacultural facility and aquacultural method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |