CN105601642A - Method for extracting artemisinin by decoction - Google Patents
Method for extracting artemisinin by decoction Download PDFInfo
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- CN105601642A CN105601642A CN201610155481.2A CN201610155481A CN105601642A CN 105601642 A CN105601642 A CN 105601642A CN 201610155481 A CN201610155481 A CN 201610155481A CN 105601642 A CN105601642 A CN 105601642A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
- C07D493/12—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains three hetero rings
- C07D493/20—Spiro-condensed systems
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Abstract
The invention discloses a method for extracting artemisinin by decoction, and belongs to the technical field of artemisinin preparation. The method comprises the following steps: soaking artemisia annua seeds, and leaching the artemisia annua seeds by utilizing an artemisinin solution which is extracted from artemisia apiacea with diethyl ether to sprout the artemisia annua seeds; then, planting the sprouted artemisinin seeds into soil, and irrigating the artemisinin seeds with an artemisinin solution which is extracted from artemisia apiacea with ethanol to enable the artemisinin seeds to come into leaves and seedlings; next, spraying the seedlings with the artemisinin solution which is extracted from artemisia apiacea with microwave to ripen the seedlings; and finally, transplanting ripe artemisia annua seedlings into desert to grow, picking up leaves and decocting to obtain artemisinin extracted by decoction. Embodiments prove that the method can avoid addition of organic solvent in the operating process, and has an operation temperature close to normal temperature, so that artemisinin cannot generate pyrolysis and other chemical changes, and the finally obtained artemisinin has a yield of more than or equal to 85 percent and a purity of more than or equal to 95 percent.
Description
Technical field
The invention discloses a kind of method that is suitable for decocting extraction qinghaosu, belong to qinghaosu preparing technical field.
Background technology
Qinghaosu is a kind of colourless acicular crystal, and molecular formula is C15H2205. Within 1976, prove that by chemical reaction, spectroscopic data and X ray single crystal diffraction method it is a kind of sesquiterpene lactone that contains peroxy. Because it has special peroxy-radical, it is to thermally labile, is subject to wet, the impact of heat and reducing substances and decomposing. Qinghaosu is to extract and obtain from compound inflorescence plant Artemisia annua grass, be used as medicine and see be the earliest the unearthed book copied on silk " 52 Bingfang " of (B.C. about 168 years) of No. three, Mawangdui tomb of Han dynasty, after all on the books in the book such as Shennong's Herbal, Compendium of Material Medica. Within 1972, China scientist isolates this active material on the basis that arranges folk remedy and practice from sweet wormwood, be a kind of brand-new antimalarial effective monomer that the distinctive plant resources artemisia annua of China is succeeded in developing, its discovery becomes another important milestone after quinine in world's antimalarial history. Due to its structure uniqueness, antimalarial mechanism is special, and malignant malaria and the brain malaria of antagonism chloroquine have special efficacy, and have the feature of high-efficiency low-toxicity, and is called " in the world at present unique effective malaria treatment medicine " by WHO.
The preparation method of qinghaosu mainly comprises chemical synthesis, biosynthesis, Plant Tissue Breeding synthetic artemisinin and from sweet wormwood plant, extracts qinghaosu. At present, natural extraction remains most widely used qinghaosu preparation method. The method of natural extraction mainly includes machine solvent extraction, supercritical CO2The methods such as extraction, microwave abstracting.
(1) technique of extracting qinghaosu in current domestic artemisia annua is mainly traditional organic solvent extraction separation, adopt organic solvent as ethanol, acetone, ether etc. extract and obtain after extract medicinal extract artemisia annua, obtain qinghaosu sterling through macroreticular resin or silicagel column fractional crystallization again, this technical matters is simply ripe, and domestic most qinghaosu manufacturing enterprise all continues to use the extracting method of this classics;
(2) supercritical carbon dioxide extraction method also claims green extraction method, and this method is widely used for the extraction of spices, has that environment is good, handling safety, does not have that pest is residual, product quality is high and can keep the features such as intrinsic smell;
(3) microwave abstracting is to utilize the effect of electromagnetic field that some organic components in solid or semi-solid material is separated with matrix is effective, and can keep a kind of separation method of the script compound state of analytic target. Microwave refers to the electromagnetic wave of frequency in 300 megahertz to 300 gigahertzs.
Traditional organic solvent extraction method, recovery rate is low, and the time is long, and organic solvent use amount is large, and impurity is more; Supercritical CO2Abstraction technique also has its shortcoming, is applicable to the extraction of lipophilicity, molecular weight material; Large to polarity, when the separation and Extraction of the material that molecular weight is too large, to add entrainer, and carry out under very high pressure, to industrialization bring certain difficulty and cost higher; Microwave Extraction can be saved a large amount of solvents, and the time is short, but the shortcoming of its existence is to need polar solvent, equipment comparatively expensive.
Therefore, finding the qinghaosu extracting method that a kind of extraction efficiency is high, artemislnin content is high, solvent use amount is low, drug effect retentivity is good, be beneficial to commercial Application, is that the problem solving is endeavoured in this area all the time.
Summary of the invention
The technical problem that the present invention mainly solves: for current conventional method extracting in the process of qinghaosu, often adopt organic solvent as ether, acetone extraction, not only energy resource consumption is large, recovery rate is low, and do not destroying the property of medicine, do not destroy in the situation of structure, be merely able to carry out under the condition of low temperature, cause long present situation of extraction time, providing a kind of passes through artemisia annua seed after immersion, utilize ether from sweet wormwood, to extract the qinghaosu solution obtaining it is carried out to drip washing, make its germination, subsequently the qinghaosu seed after germinateing is implanted in soil, from sweet wormwood, extract the qinghaosu solution obtaining waters it with ethanol, it is come into leaves, become seedling, next the qinghaosu solution obtaining from sweet wormwood by Microwave Extraction is again sprayed to seedling, make its maturation, finally by ripe artemisia annua seedling is transferred in desert and is grown, and win leaf and decoct, thereby obtain being suitable for the method for decocting extraction qinghaosu. the method is unique novel, raw material is natural to be easy to get, not only in operating process, avoid adding of organic solvent, natural safety, and also the cycle is short, operating temperature approaches normal temperature, can there is not the chemical changes such as cracking in qinghaosu, make the qinghaosu yield finally obtaining up to more than 85%, and purity reaches more than 95%, there is good prospects for commercial application, be applicable to large-scale promotion application.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:
(1) take 80～100g artemisia annua grass seed, be placed in the container that fills clear water, constantly stir, soak 45～55min, wait to soak and finish, remove the seed that swims in the water surface, leach residue seed, after repeatedly rinsing with clear water, put into the beaker that 30～35 DEG C of warm water are housed, soak 4～5h, wait to soak and finish, artemisia annua grass seed after soaking is taken out, be evenly laid in the culture dish that is lined with the rear gauze of sterilization;
(2) put into drip washing tank by extract the qinghaosu solution obtaining from sweet wormwood by ether, after regulating pH value to be 5.5～6.2, every other day the artemisia annua grass seed in above-mentioned culture dish is carried out to drip washing, drip washing 5～10 days, make artemisia annua grass seed plumule reach 2～3cm, again regulating subsequently leacheate pH value is 5.8～6.3, every 5h, the artemisia annua seed having sprouted is carried out to drip washing, make it continue to germinate, treat artemisia annua seed complete germination, when plumule reaches 5～6cm, stop drip washing, with clear water sprinkling chitting piece surface, sprayed once every 1～2 day, altogether spray 2～4 times,
(3) treat that above-mentioned sprinkling finishes, the artemisia annua seed that grows plumule is adopted to plastic film mulching cultivation, evenly move in seedling canopy, between every seed, be spaced apart 30～35cm, after transplanting completes, on seedling canopy, add lid layer shading rate and be 75% sunshade net, controlling seedling canopy indoor temperature is 25～28 DEG C, keeping soil moisture is 75～85%, and use and from sweet wormwood, extract the qinghaosu solution pouring artemisia annua seed plumule obtaining by ethanol, 8 pourings every morning once, are watered once it at 5 in afternoon again;
(4) treat that above-mentioned artemisia annua seed plumule grows blue or green tender leaf, while becoming seedling, stop pouring, after self-sow 3～5 days, use the qinghaosu solution obtaining from sweet wormwood by Microwave Extraction to spray to seedling, spray every day 1 time, spray continuously 10～15 days, make the rejuvenation of artemisia annua seedling, grow dark green ripe leaf;
(5) centered by above-mentioned mature artemisia annua young plant, taking a radius is 10～15cm, be the circular groove of 15～20cm deeply, artemisia annua young plant is connected to root band mud have been dug, move into subsequently in desert, root soil compaction, and water with clear water, continue pouring and after 5～8 days, stop pouring, make its self-sow;
(6) artemisia annua in desert grows to while reaching 3～4cm up to 100～150cm, base diameter, can win artemisia annua leaf, put into saucepan, and add wherein clear water, make it soak artemisia annua leaf completely, at 80～90 DEG C, decoct 1～3h, can under the condition of decocting, obtain qinghaosu.
Application process of the present invention is: the qinghaosu that the present invention is made is put into baking oven, at 100～105 DEG C of temperature, dry to constant weight, obtain qinghaosu powder, subsequently the qinghaosu powder obtaining is made to qinghaosu capsule by capsule machine, wherein in each capsule, contain 0.1～0.3g qinghaosu, while taking, be equipped with 200～250mL warm water, morning and evening every day one after each meal once, each serving with 1～3, adhere to taking 7～14 days, can eliminate rapidly plasmodium in human body, pernicious malaria is had to good result for the treatment of.
The invention has the beneficial effects as follows:
(1) the present invention is easy and simple to handle, avoids adding of organic solvent in preparation process, and safety and sanitation are environment-friendly and green production technologies;
(2) operation cycle of the present invention short, operating temperature approaches normal temperature, can there is not the chemical changes such as cracking in qinghaosu, kept original drug effect, and without any impurity;
(3) the present invention finally makes qinghaosu yield up to more than 85%, and purity reaches more than 95%, has good prospects for commercial application, is applicable to large-scale promotion application.
Detailed description of the invention
First take 80～100g artemisia annua grass seed, be placed in the container that fills clear water, constantly stir, soak 45～55min, wait to soak and finish, remove the seed that swims in the water surface, leach residue seed, after repeatedly rinsing with clear water, put into the beaker that 30～35 DEG C of warm water are housed, soak 4～5h, wait to soak and finish, artemisia annua grass seed after soaking is taken out, be evenly laid in the culture dish that is lined with the rear gauze of sterilization, then put into drip washing tank by extract the qinghaosu solution obtaining from sweet wormwood by ether, after regulating pH value to be 5.5～6.2, every other day the artemisia annua grass seed in above-mentioned culture dish is carried out to drip washing, drip washing 5～10 days, make artemisia annua grass seed plumule reach 2～3cm, again regulating subsequently leacheate pH value is 5.8～6.3, every 5h, the artemisia annua seed having sprouted is carried out to drip washing, make it continue to germinate, treat artemisia annua seed complete germination, when plumule reaches 5～6cm, stop drip washing, with clear water sprinkling chitting piece surface, sprayed once every 1～2 day, altogether spray 2～4 times, treat that above-mentioned sprinkling finishes, the artemisia annua seed that grows plumule is adopted to plastic film mulching cultivation, evenly move in seedling canopy, between every seed, be spaced apart 30～35cm, after having transplanted, on seedling canopy, add lid layer shading rate and be 75% sunshade net, controlling seedling canopy indoor temperature is 25～28 DEG C, and keeping soil moisture is 75～85%, and uses and from sweet wormwood, extract the qinghaosu solution pouring artemisia annua seed plumule obtaining by ethanol, 8 pourings every morning once, are watered once it at 5 in afternoon again, treat that above-mentioned artemisia annua seed plumule grows blue or green tender leaf, while becoming seedling, stop pouring, after self-sow 3～5 days, use the qinghaosu solution obtaining from sweet wormwood by Microwave Extraction to spray to seedling, spray every day 1 time, spray continuously 10～15 days, make the rejuvenation of artemisia annua seedling, grow dark green ripe leaf, next centered by above-mentioned mature artemisia annua young plant, taking a radius is 10～15cm, be the circular groove of 15～20cm deeply, artemisia annua young plant is connected to root band mud have been dug, move into subsequently in desert, root soil compaction, and water with clear water, continue pouring and after 5～8 days, stop pouring, make its self-sow, finally the artemisia annua in desert grows to while reaching 3～4cm up to 100～150cm, base diameter, can win artemisia annua leaf, put into saucepan, and add wherein clear water, make it soak artemisia annua leaf completely, at 80～90 DEG C, decoct 1～3h, can under the condition of decocting, obtain qinghaosu.
Example 1
First take 80g artemisia annua grass seed, be placed in the container that fills clear water, constantly stir, soak 45min, wait to soak and finish, remove the seed that swims in the water surface, leach residue seed, after repeatedly rinsing with clear water, put into the beaker that 30 DEG C of warm water are housed, soak 4h, wait to soak and finish, artemisia annua grass seed after soaking is taken out, be evenly laid in the culture dish that is lined with the rear gauze of sterilization; Then put into drip washing tank by extract the qinghaosu solution obtaining from sweet wormwood by ether, after regulating pH value to be 5.5, every other day the artemisia annua grass seed in above-mentioned culture dish is carried out to drip washing, drip washing 5 days, make artemisia annua grass seed plumule reach 2cm, again regulating subsequently leacheate pH value is 5.8, every 5h, the artemisia annua seed having sprouted is carried out to drip washing, make it continue to germinate, treat artemisia annua seed complete germination, when plumule reaches 5cm, stop drip washing, with clear water sprinkling chitting piece surface, sprayed once every 1 day, altogether spray 2 times; Treat that above-mentioned sprinkling finishes, the artemisia annua seed that grows plumule is adopted to plastic film mulching cultivation, evenly move in seedling canopy, between every seed, be spaced apart 30cm, after having transplanted, on seedling canopy, add lid layer shading rate and be 75% sunshade net, controlling seedling canopy indoor temperature is 25 DEG C, and keeping soil moisture is 75%, and uses and from sweet wormwood, extract the qinghaosu solution pouring artemisia annua seed plumule obtaining by ethanol, 8 pourings every morning once, are watered once it at 5 in afternoon again; Treat that above-mentioned artemisia annua seed plumule grows blue or green tender leaf, while becoming seedling, stop pouring, after self-sow 3 days, use the qinghaosu solution obtaining from sweet wormwood by Microwave Extraction to spray to seedling, spray every day 1 time, spray continuously 10 days, make the rejuvenation of artemisia annua seedling, grow dark green ripe leaf; Next centered by above-mentioned mature artemisia annua young plant, taking a radius is 10cm, is the circular groove of 15cm deeply, artemisia annua young plant is connected to root band mud and dug, move into subsequently in desert root soil compaction, and with clear water pouring, continue pouring and after 5 days, stop pouring, make its self-sow; Finally the artemisia annua in desert grows to while reaching 3cm up to 100cm, base diameter, can win artemisia annua leaf, puts into saucepan, and add wherein clear water, make it soak artemisia annua leaf completely, at 80 DEG C, decoct 1h, can under the condition of decocting, obtain qinghaosu.
This example operation is simple, and when use, the qinghaosu that the present invention is made is put into baking oven, at 100 DEG C of temperature, dry to constant weight, obtain qinghaosu powder, subsequently the qinghaosu powder obtaining is made to qinghaosu capsule by capsule machine, wherein in each capsule, contain 0.1g qinghaosu, while taking, be equipped with 200mL warm water, morning and evening every day one after each meal once, each serving with 1, adhere to taking 7 days, can eliminate rapidly plasmodium in human body, pernicious malaria is had to good result for the treatment of.
Example 2
First take 90g artemisia annua grass seed, be placed in the container that fills clear water, constantly stir, soak 50min, wait to soak and finish, remove the seed that swims in the water surface, leach residue seed, after repeatedly rinsing with clear water, put into the beaker that 33 DEG C of warm water are housed, soak 4.5h, wait to soak and finish, artemisia annua grass seed after soaking is taken out, be evenly laid in the culture dish that is lined with the rear gauze of sterilization; Then put into drip washing tank by extract the qinghaosu solution obtaining from sweet wormwood by ether, after regulating pH value to be 5.8, every other day the artemisia annua grass seed in above-mentioned culture dish is carried out to drip washing, drip washing 8 days, make artemisia annua grass seed plumule reach 2.5cm, again regulating subsequently leacheate pH value is 6.2, every 5h, the artemisia annua seed having sprouted is carried out to drip washing, make it continue to germinate, treat artemisia annua seed complete germination, when plumule reaches 5.5cm, stop drip washing, with clear water sprinkling chitting piece surface, sprayed once every 1.5 days, altogether spray 3 times; Treat that above-mentioned sprinkling finishes, the artemisia annua seed that grows plumule is adopted to plastic film mulching cultivation, evenly move in seedling canopy, between every seed, be spaced apart 33cm, after having transplanted, on seedling canopy, add lid layer shading rate and be 75% sunshade net, controlling seedling canopy indoor temperature is 27 DEG C, and keeping soil moisture is 80%, and uses and from sweet wormwood, extract the qinghaosu solution pouring artemisia annua seed plumule obtaining by ethanol, 8 pourings every morning once, are watered once it at 5 in afternoon again; Treat that above-mentioned artemisia annua seed plumule grows blue or green tender leaf, while becoming seedling, stop pouring, after self-sow 4 days, use the qinghaosu solution obtaining from sweet wormwood by Microwave Extraction to spray to seedling, spray every day 1 time, spray continuously 13 days, make the rejuvenation of artemisia annua seedling, grow dark green ripe leaf; Next centered by above-mentioned mature artemisia annua young plant, taking a radius is 13cm, is the circular groove of 18cm deeply, artemisia annua young plant is connected to root band mud and dug, move into subsequently in desert root soil compaction, and with clear water pouring, continue pouring and after 7 days, stop pouring, make its self-sow; Finally the artemisia annua in desert grows to while reaching 3.5cm up to 130cm, base diameter, can win artemisia annua leaf, puts into saucepan, and add wherein clear water, make it soak artemisia annua leaf completely, at 85 DEG C, decoct 2h, can under the condition of decocting, obtain qinghaosu.
This example operation is simple, and when use, the qinghaosu that the present invention is made is put into baking oven, at 103 DEG C of temperature, dry to constant weight, obtain qinghaosu powder, subsequently the qinghaosu powder obtaining is made to qinghaosu capsule by capsule machine, wherein in each capsule, contain 0.2g qinghaosu, while taking, be equipped with 230mL warm water, morning and evening every day one after each meal once, each serving with 2, adhere to taking 10 days, can eliminate rapidly plasmodium in human body, pernicious malaria is had to good result for the treatment of.
Example 3
First take 100g artemisia annua grass seed, be placed in the container that fills clear water, constantly stir, soak 55min, wait to soak and finish, remove the seed that swims in the water surface, leach residue seed, after repeatedly rinsing with clear water, put into the beaker that 35 DEG C of warm water are housed, soak 5h, wait to soak and finish, artemisia annua grass seed after soaking is taken out, be evenly laid in the culture dish that is lined with the rear gauze of sterilization; Then put into drip washing tank by extract the qinghaosu solution obtaining from sweet wormwood by ether, after regulating pH value to be 6.2, every other day the artemisia annua grass seed in above-mentioned culture dish is carried out to drip washing, drip washing 10 days, make artemisia annua grass seed plumule reach 3cm, again regulating subsequently leacheate pH value is 6.3, every 5h, the artemisia annua seed having sprouted is carried out to drip washing, make it continue to germinate, treat artemisia annua seed complete germination, when plumule reaches 6cm, stop drip washing, with clear water sprinkling chitting piece surface, sprayed once every 2 days, altogether spray 4 times; Treat that above-mentioned sprinkling finishes, the artemisia annua seed that grows plumule is adopted to plastic film mulching cultivation, evenly move in seedling canopy, between every seed, be spaced apart 35cm, after having transplanted, on seedling canopy, add lid layer shading rate and be 75% sunshade net, controlling seedling canopy indoor temperature is 28 DEG C, and keeping soil moisture is 85%, and uses and from sweet wormwood, extract the qinghaosu solution pouring artemisia annua seed plumule obtaining by ethanol, 8 pourings every morning once, are watered once it at 5 in afternoon again; Treat that above-mentioned artemisia annua seed plumule grows blue or green tender leaf, while becoming seedling, stop pouring, after self-sow 5 days, use the qinghaosu solution obtaining from sweet wormwood by Microwave Extraction to spray to seedling, spray every day 1 time, spray continuously 15 days, make the rejuvenation of artemisia annua seedling, grow dark green ripe leaf; Next centered by above-mentioned mature artemisia annua young plant, taking a radius is 15cm, is the circular groove of 20cm deeply, artemisia annua young plant is connected to root band mud and dug, move into subsequently in desert root soil compaction, and with clear water pouring, continue pouring and after 8 days, stop pouring, make its self-sow; Finally the artemisia annua in desert grows to while reaching 4cm up to 150cm, base diameter, can win artemisia annua leaf, puts into saucepan, and add wherein clear water, make it soak artemisia annua leaf completely, at 90 DEG C, decoct 3h, can under the condition of decocting, obtain qinghaosu.
This example operation is simple, and when use, the qinghaosu that the present invention is made is put into baking oven, at 105 DEG C of temperature, dry to constant weight, obtain qinghaosu powder, subsequently the qinghaosu powder obtaining is made to qinghaosu capsule by capsule machine, wherein in each capsule, contain 0.3g qinghaosu, while taking, be equipped with 250mL warm water, morning and evening every day one after each meal once, each serving with 3, adhere to taking 14 days, can eliminate rapidly plasmodium in human body, pernicious malaria is had to good result for the treatment of.
Claims (1)
1. be suitable for the method that decocting extracts qinghaosu, it is characterized in that concrete operation step is:
(1) take 80～100g artemisia annua grass seed, be placed in the container that fills clear water, constantly stir, soak 45～55min, wait to soak and finish, remove the seed that swims in the water surface, leach residue seed, after repeatedly rinsing with clear water, put into the beaker that 30～35 DEG C of warm water are housed, soak 4～5h, wait to soak and finish, artemisia annua grass seed after soaking is taken out, be evenly laid in the culture dish that is lined with the rear gauze of sterilization;
(2) put into drip washing tank by extract the qinghaosu solution obtaining from sweet wormwood by ether, after regulating pH value to be 5.5～6.2, every other day the artemisia annua grass seed in above-mentioned culture dish is carried out to drip washing, drip washing 5～10 days, make artemisia annua grass seed plumule reach 2～3cm, again regulating subsequently leacheate pH value is 5.8～6.3, every 5h, the artemisia annua seed having sprouted is carried out to drip washing, make it continue to germinate, treat artemisia annua seed complete germination, when plumule reaches 5～6cm, stop drip washing, with clear water sprinkling chitting piece surface, sprayed once every 1～2 day, altogether spray 2～4 times,
(3) treat that above-mentioned sprinkling finishes, the artemisia annua seed that grows plumule is adopted to plastic film mulching cultivation, evenly move in seedling canopy, between every seed, be spaced apart 30～35cm, after transplanting completes, on seedling canopy, add lid layer shading rate and be 75% sunshade net, controlling seedling canopy indoor temperature is 25～28 DEG C, keeping soil moisture is 75～85%, and use and from sweet wormwood, extract the qinghaosu solution pouring artemisia annua seed plumule obtaining by ethanol, 8 pourings every morning once, are watered once it at 5 in afternoon again;
(4) treat that above-mentioned artemisia annua seed plumule grows blue or green tender leaf, while becoming seedling, stop pouring, after self-sow 3～5 days, use the qinghaosu solution obtaining from sweet wormwood by Microwave Extraction to spray to seedling, spray every day 1 time, spray continuously 10～15 days, make the rejuvenation of artemisia annua seedling, grow dark green ripe leaf;
(5) centered by above-mentioned mature artemisia annua young plant, taking a radius is 10～15cm, be the circular groove of 15～20cm deeply, artemisia annua young plant is connected to root band mud have been dug, move into subsequently in desert, root soil compaction, and water with clear water, continue pouring and after 5～8 days, stop pouring, make its self-sow;
(6) artemisia annua in desert grows to while reaching 3～4cm up to 100～150cm, base diameter, can win artemisia annua leaf, put into saucepan, and add wherein clear water, make it soak artemisia annua leaf completely, at 80～90 DEG C, decoct 1～3h, can under the condition of decocting, obtain qinghaosu.
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| CN201610155481.2A CN105601642A (en) | 2016-03-18 | 2016-03-18 | Method for extracting artemisinin by decoction |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107383051A (en) * | 2017-06-21 | 2017-11-24 | 禹州市天源生物科技有限公司 | A kind of technique of decocting extraction qinghaosu |
| CN107912200A (en) * | 2017-12-13 | 2018-04-17 | 曾少兰 | A kind of control method of guava pest and disease damage |
-
2016
- 2016-03-18 CN CN201610155481.2A patent/CN105601642A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107383051A (en) * | 2017-06-21 | 2017-11-24 | 禹州市天源生物科技有限公司 | A kind of technique of decocting extraction qinghaosu |
| CN107912200A (en) * | 2017-12-13 | 2018-04-17 | 曾少兰 | A kind of control method of guava pest and disease damage |
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