CN105494453B - Preparation method of radix pseudostellariae seed soaking solution - Google Patents
Preparation method of radix pseudostellariae seed soaking solution Download PDFInfo
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- CN105494453B CN105494453B CN201610062532.7A CN201610062532A CN105494453B CN 105494453 B CN105494453 B CN 105494453B CN 201610062532 A CN201610062532 A CN 201610062532A CN 105494453 B CN105494453 B CN 105494453B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Soil Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
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Abstract
The invention discloses a radix pseudostellariae seed soaking solution and a preparation method and a use method thereof, and relates to the technical field of biological cultivation. The invention relates to a liquid medicine with both fertilizer and medicine prepared by using ginseng, honeysuckle and sheep manure as main raw materials. The preparation method of the soaking solution comprises six steps of radix pseudostellariae powder preparation, honeysuckle powder preparation, sheep manure powder preparation, mixing, fermentation and soaking solution preparation, and the soaking solution is used by three steps of seed pretreatment, soaking and sowing. The invention has obvious detoxification effect, is simple and feasible, and is suitable for popularization in broad farmers.
Description
Technical field
The present invention relates to biological technical field of cultivation, in particular to a kind of preparation method of radix pseudostellariae seed soak.
Background technique
Radix pseudostellariae comes from " new compilation of materia medica ": radix pseudostellariae, though very thin, as joined item, short tight knot is real, and has Lu Wen, and power is not lower big
Ginseng.According to the books such as " new compilation of materia medica ", " detailed outline is picked up any lost article from the road ", " medicine materical crude slice is newly joined ", radix pseudostellariae original refers to the small person of Araliaceae ginseng.Now
Commodity then generally use the root tuber of pinkwort pseudostellaria heterophylla, though there is nourishing function, its power is relatively thin.Its property is sweet, mild-natured, micro-
It is bitter;Enter the warp of spleen and lung two, the effect of replenishing qi to invigorate the spleen, is promoting production of body fluid and nourishing the lung, for treating tired spleen deficiency body, loss of appetite, after being ill void
The illnesss such as weak, deficiency of both vital energy and Yin, spontaneous perspiration dry, dryness of the lung dry cough.It clinically can be used alone or be used cooperatively with other Chinese medicines, or use
In prescribed preparation.Clinic shows in recent years, and radix pseudostellariae is in treatment hepatitis, diabetes, coronary heart diseases and angina pectoris, secondary regeneration barrier
It is achieved in terms of the illnesss such as impenetrability anaemia, leukopenia, hyperthyroidism, scrofula and a variety of epidemic infectious diseases of prevention new
Progress, now again useful radix pseudostellariae for health food, cosmetics develop, dosage speed increase.Radix pseudostellariae main product in Jiangsu,
Shandong, Anhui and Fujian etc. save, and have the cultivation history of last 100 years, especially preferable with Fujian Zherong radix pseudostellariae quality, crown prince in China
Ginseng has become Zherong pillar industry, and 3.2 ten thousand mu of whole county radix pseudostellariae cultivated area, more than 4900 tons of annual output, volume of production and marketing accounts for three points of the whole nation
Two.Due to long-term vegetative propagation, a variety of viruses, aggrieved plant leaf shrinkage and floral leaf are disseminated and had accumulated in radix pseudostellariae body,
This disease of plant occurs generally in producing region, and disease incidence is up to 70%, seriously endangers, influences the yield and quality of radix pseudostellariae.It is reported that main
To contain tobacco mosaic virus (TMV), 4 kinds of Brassica 2 et 4, cucumber mosaic virus and broad bean wilt virus viruses.At present for too
There are no effective drugs for the prevention and treatment of son ginseng virosis, and obtaining virus-free plant by induction radix pseudostellariae shoot apical meristem is
The most effective approach for removing radix pseudostellariae virosis, having done this respect if the patent of invention application No. is 201110434688.0
Effort, however tissue culture method needs to put into a large amount of equipment and fund, and the period is long, technical requirements are high, is not easy domestication popularization, because
It is unpractical that this tissue cultures for the vast peasant household in economic low layer, which obtains detoxification radix pseudostellariae plant,.In addition,
Radix pseudostellariae prevention and treatment virosis mostly uses soil crop rotation or the reclamation of wasteland newly to plant, but can not all eradicate virosis.Thus study one kind
It is simple and easy, it is suitable for being promoted in wide farmers, the significant radix pseudostellariae seed treatment of detoxification efficiency and preparation method will have huge
Big market popularization value.
Summary of the invention
The present invention provides a kind of preparation methods of radix pseudostellariae seed soak, overcome existing tissue culture to obtain detoxic seedling and deposit
In equipment and capital investment is big, the period is long, technical requirements are high, is not easy the defect of domestication popularization, and traditional soil crop rotation or the reclamation of wasteland
The defect that can not eradicate virosis newly is planted, provides a kind of simple and easy, is suitable for being promoted in wide farmers, detoxification efficiency
The preparation method of significant radix pseudostellariae seed soak.
The present invention is achieved through the following technical solutions: a kind of radix pseudostellariae seed soak is with radix pseudostellariae, gold and silver
Flower, sheep dung are the medical fluid that the fertile medicine of primary raw material preparation has both.
The preparation method of above-mentioned radix pseudostellariae seed soak, includes the following steps:
(1) prepared by radix pseudostellariae powder: fresh radix pseudostellariae being cleaned and is dried to dehydration rate and reaches 90% or more, is put into and is pulverized
It is pulverized in machine, and screens out exceeded product with 200 mesh screens and obtain radix pseudostellariae powder；
(2) prepared by honeysuckle powder: dried honeysuckle product of the water content lower than 10% will be chosen, is put into crusher and is pulverized,
And exceeded product is screened out with 120 ~ 150 mesh screens and obtains honeysuckle powder；
(3) prepared by sheep dung powder: the concentration of 2 times of its weight is added for 75% alcohol, with sterile after 8 ~ 10h of immersion in sheep dung
Water rinses 2-3 times, is dried to that dehydration rate reaches between 90 ~ 95%, is put into crusher and pulverized, and with 80 ~ 100 meshes
Mesh screen obtains sheep dung powder except exceeded product；
(4) it mixes: the sheep of honeysuckle powder, step (3) acquisition that radix pseudostellariae powder, the step (2) that step (1) is obtained obtain
Excrement powder is mixed evenly according to the ratio of 2:1:7, and the alcohol that the concentration for being added 2 times of mixture weight is 98% impregnates 4 ~ 6h,
40 DEG C are heated to, is played completely to alcohol, 1 times of remaining weight percentage of sterile water is added and obtains soak；
(5) it ferments: by the microbe leaven of total weight 1/100 is added in soak obtained by step (4), putting to temperature and be
Ferment 20h in 25 ~ 28 ° of room, and impurity screening extracts fermentation liquid；
(6) soak is prepared: being prepared according to the ratio of 1 part of fermentation liquid obtained by step (5), 9 parts of water up to soak.
The application method of the radix pseudostellariae seed soak, includes the following steps:
(1) Seeds preprocess: before sowing, radix pseudostellariae root tuber or seed that seed-plot has just been dug out place shine 2 under the sun ~
3h is down to 80% ± 5% to water content；
(2) it impregnates: root tuber obtained by step (1) or seed being put into the cement pit or bucket for having configured soak in advance, institute
The ratio for stating soak and root tuber or seed is 2:1, soaking time 6-8h；
(3) it sows: step (2) soaked seed being seeded in soil, is preferably seeded in continuous cropping 3 years or more soils.
Compared with prior art, the present invention has the advantages that following prominent and effect: (1) water extract of radix pseudostellariae, alcohol extracting
Object can be by removing oxygen anion free radical (o2 -), hydroxy radical (OH), improve superoxide dismutase and glutathione mistake
Oxygen object enzyme activity and play antioxidant activity and anti-inflammatory bactericidal effect, the alcohol extract main component of honeysuckle is chlorogenic acid, is also had
There are antibacterial and anti-inflammation functions, Chinese medicine of the present invention using both the above with anti-inflammatory bactericidal effect, which combines to have as main component, improves soil
Matter, prevent the hardening soil act on sheep dung, carry out alcohol extracting after three is mixed, after ethyl alcohol is volatilized add microbe leaven into
It goes and ferments, further adding immersion made of human urine is the medical fluid that liquid fertilizer has both, and anti-inflammatory sterilization component therein can penetrate into
Impregnate radix pseudostellariae in include virus including all microorganisms, destroy microorganism in enzyme activity, inhibit its protein synthesize etc.
Step, to have the function that antiviral, sterilization；And the fertilizer efficiency ingredient in soak can for impregnate radix pseudostellariae provide nutrition at
Point, it plays strengthening root, raising vigor, effectively resist pest and disease damage, thus detoxification efficiency is significant；(2) the invention is simple and feasible, be suitable for
It is promoted in wide farmers.
Specific embodiment
Following Examples further illustrate the present invention, but should not be taken as limitation of the invention:
Embodiment 1: a kind of radix pseudostellariae seed soak is the fertilizer prepared using radix pseudostellariae, honeysuckle, sheep dung as primary raw material
The medical fluid that medicine has both.
The preparation method of above-mentioned radix pseudostellariae seed soak, includes the following steps:
(1) prepared by radix pseudostellariae powder: fresh radix pseudostellariae being cleaned and is dried to dehydration rate and reaches 90% or more, is put into and is pulverized
It is pulverized in machine, and screens out exceeded product with 200 mesh screens and obtain radix pseudostellariae powder；
(2) prepared by honeysuckle powder: dried honeysuckle product of the water content lower than 10% will be chosen, is put into crusher and is pulverized,
And exceeded product is screened out with 120 ~ 150 mesh screens and obtains honeysuckle powder；
(3) prepared by sheep dung powder: the alcohol that the concentration of 2 times of its weight is 75% is added in sheep dung, is rushed after impregnating 8h with sterile water
It washes 2 times, is dried to that dehydration rate reaches between 90 ~ 95%, is put into crusher and pulverized, and screened out with 80 ~ 100 mesh screens
Exceeded product obtains sheep dung powder；
(4) it mixes: the sheep of honeysuckle powder, step (3) acquisition that radix pseudostellariae powder, the step (2) that step (1) is obtained obtain
Excrement powder is mixed evenly according to the ratio of 2:1:7, and the alcohol that the concentration for being added 2 times of mixture weight is 98% impregnates 6h, adds
Heat plays completely to 40 DEG C to alcohol, and 1 times of remaining weight percentage of sterile water is added and obtains soak；
(5) it ferments: by the microbe leaven of total weight 1/100 is added in soak obtained by step (4), putting to temperature and be
Ferment 20h in 25 ° of room, and impurity screening extracts fermentation liquid；
(6) soak is prepared: being prepared according to the ratio of 1 part of fermentation liquid obtained by step (5), 9 parts of water up to soak.
The application method of the radix pseudostellariae seed soak, includes the following steps:
(1) Seeds preprocess: before sowing, radix pseudostellariae root tuber or seed that seed-plot has just been dug out place shine 2 under the sun ~
3h is down to 80% ± 5% to water content；
(2) it impregnates: root tuber obtained by step (1) or seed being put into the cement pit or bucket for having configured soak in advance, institute
The ratio for stating soak and root tuber or seed is 2:1, soaking time 6-8h；
(3) it sows: step (2) soaked seed is seeded in the soil of continuous cropping 4 years.
Embodiment 2: a kind of radix pseudostellariae seed soak is the fertilizer prepared using radix pseudostellariae, honeysuckle, sheep dung as primary raw material
The medical fluid that medicine has both.
The preparation method of above-mentioned radix pseudostellariae seed soak, includes the following steps:
(1) prepared by radix pseudostellariae powder: fresh radix pseudostellariae being cleaned and is dried to dehydration rate and reaches 90% or more, is put into and is pulverized
It is pulverized in machine, and screens out exceeded product with 200 mesh screens and obtain radix pseudostellariae powder；
(2) prepared by honeysuckle powder: dried honeysuckle product of the water content lower than 10% will be chosen, is put into crusher and is pulverized,
And exceeded product is screened out with 120 ~ 150 mesh screens and obtains honeysuckle powder；
(3) prepared by sheep dung powder: the alcohol that the concentration of 2 times of its weight is 75% is added in sheep dung, uses sterile water after impregnating 10h
It rinses 3 times, is dried to that dehydration rate reaches between 90 ~ 95%, is put into crusher and pulverized, and with 80 ~ 100 mesh mesh screens
Except exceeded product obtains sheep dung powder；
(4) it mixes: the sheep of honeysuckle powder, step (3) acquisition that radix pseudostellariae powder, the step (2) that step (1) is obtained obtain
Excrement powder is mixed evenly according to the ratio of 2:1:7, and the alcohol that the concentration for being added 2 times of mixture weight is 98% impregnates 4h, adds
Heat plays completely to 40 DEG C to alcohol, and 1 times of remaining weight percentage of sterile water is added and obtains soak；
(5) it ferments: by the microbe leaven of total weight 1/100 is added in soak obtained by step (4), putting to temperature and be
Ferment 20h in 28 ° of room, and impurity screening extracts fermentation liquid；
(6) soak is prepared: being prepared according to the ratio of 1 part of fermentation liquid obtained by step (5), 9 parts of water up to soak.
The application method of the radix pseudostellariae seed soak, includes the following steps:
(1) Seeds preprocess: before sowing, radix pseudostellariae root tuber or seed that seed-plot has just been dug out is placed and shone under the sun
3h is down to 80% ± 5% to water content；
(2) it impregnates: root tuber obtained by step (1) or seed being put into the cement pit or bucket for having configured soak in advance, institute
The ratio for stating soak and root tuber or seed is 2:1, soaking time 6-8h；
(3) it sows: step (2) soaked seed is seeded in the soil of continuous cropping 3 years.
Embodiment 3: a kind of radix pseudostellariae seed soak is the fertilizer prepared using radix pseudostellariae, honeysuckle, sheep dung as primary raw material
The medical fluid that medicine has both.
The preparation method of above-mentioned radix pseudostellariae seed soak, includes the following steps:
(1) prepared by radix pseudostellariae powder: fresh radix pseudostellariae being cleaned and is dried to dehydration rate and reaches 90% or more, is put into and is pulverized
It is pulverized in machine, and screens out exceeded product with 200 mesh screens and obtain radix pseudostellariae powder；
(2) prepared by honeysuckle powder: dried honeysuckle product of the water content lower than 10% will be chosen, is put into crusher and is pulverized,
And exceeded product is screened out with 120 ~ 150 mesh screens and obtains honeysuckle powder；
(3) prepared by sheep dung powder: the alcohol that the concentration of 2 times of its weight is 75% is added in sheep dung, is rushed after impregnating 9h with sterile water
It washes 3 times, is dried to that dehydration rate reaches between 90 ~ 95%, is put into crusher and pulverized, and screened out with 80 ~ 100 mesh screens
Exceeded product obtains sheep dung powder；
(4) it mixes: the sheep of honeysuckle powder, step (3) acquisition that radix pseudostellariae powder, the step (2) that step (1) is obtained obtain
Excrement powder is mixed evenly according to the ratio of 2:1:7, and the alcohol that the concentration for being added 2 times of mixture weight is 98% impregnates 5h, adds
Heat plays completely to 40 DEG C to alcohol, and 1 times of remaining weight percentage of sterile water is added and obtains soak；
(5) it ferments: by the microbe leaven of total weight 1/100 is added in soak obtained by step (4), putting to temperature and be
Ferment 20h in 26 ° of room, and impurity screening extracts fermentation liquid；
(6) soak is prepared: being prepared according to the ratio of 1 part of fermentation liquid obtained by step (5), 9 parts of water up to soak.
The application method of the radix pseudostellariae seed soak, includes the following steps:
(1) Seeds preprocess: before sowing, radix pseudostellariae root tuber or seed that seed-plot has just been dug out is placed and shone under the sun
3h is down to 80% ± 5% to water content；
(2) it impregnates: root tuber obtained by step (1) or seed being put into the cement pit or bucket for having configured soak in advance, institute
The ratio for stating soak and root tuber or seed is 2:1, soaking time 6-8h；
(3) it sows: step (2) soaked seed is seeded in the soil of continuous cropping 5 years.
Embodiment 4: effect compares
It is comparison with the radix pseudostellariae of unused soak immersion treatment prepared by the present invention, other planting conditions keep one
It causes, compares the virus evil incidence of radix pseudostellariae.
| Experimental group | Virus evil incidence (%) |
| It soaks out to come without soak | 75 |
| Use soak made of embodiment 1 | 24 |
| Use soak made of embodiment 2 | 22 |
| Use soak made of embodiment 3 | 23 |
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those skilled in the art within the technical scope disclosed by the invention, can without the variation that creative work is expected or
Replacement, should be covered by the protection scope of the present invention.
Claims (1)
1. a kind of preparation method of radix pseudostellariae seed soak, it is characterised in that include the following steps:
(1) prepared by radix pseudostellariae powder: fresh radix pseudostellariae being cleaned and is dried to dehydration rate and reaches 90% or more, is put into crusher
It pulverizes, and screens out exceeded product with 200 mesh screens and obtain radix pseudostellariae powder；
(2) prepared by honeysuckle powder: dried honeysuckle product of the water content lower than 10% will be chosen, is put into crusher and is pulverized, and
Exceeded product, which is screened out, with 120 ~ 150 mesh screens obtains honeysuckle powder；
(3) prepared by sheep dung powder: the alcohol that the concentration of 2 times of its weight is 75% is added in sheep dung, is rushed after impregnating 8 ~ 10h with sterile water
It washes 2-3 times, is dried to that dehydration rate reaches between 90 ~ 95%, is put into crusher and pulverized, and with 80 ~ 100 mesh mesh screens
Except exceeded product obtains sheep dung powder；
(4) it mixes: the sheep dung powder of honeysuckle powder, step (3) acquisition that radix pseudostellariae powder, the step (2) that step (1) is obtained obtain
It is mixed evenly according to the ratio of 2:1:7, the alcohol that the concentration for being added 2 times of mixture weight is 98% impregnates 4 ~ 6h, heating
It to 40 DEG C, is played completely to alcohol, 1 times of remaining weight percentage of sterile water is added and obtains soak；
(5) ferment: the microbe leaven of total weight 1/100 will be added in soak obtained by step (4), put to temperature be 25 ~
Ferment 20h in 28 ° of room, and impurity screening extracts fermentation liquid；
(6) soak is prepared: being prepared according to the ratio of 1 part of fermentation liquid obtained by step (5), 9 parts of water up to soak.
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| CN105265141A (en) * | 2015-09-28 | 2016-01-27 | 叶胜佳 | Method for artificially planting pseudostellaria heterophylla |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105265141A (en) * | 2015-09-28 | 2016-01-27 | 叶胜佳 | Method for artificially planting pseudostellaria heterophylla |
Non-Patent Citations (3)
| Title |
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| 太子参及其无公害栽培技术;吴丽华;《北京农业》;20080331;第15-16页 * |
| 太子参水浸液对其种子萌发和幼苗生长的影响;任永权等;《北方园艺》;20121231(第6期);第172-174页 * |
| 太子参种子特性及种胚离体诱导培养技术研究;叶祖云等;《种子》;20101130;第29卷(第11期);第6-13页 * |
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Address after: 352100 No. 63, Fengyang village, Fengyang village, Yingshan Township, Zherong County, Ningde City, Fujian Province Patentee after: Fujian Zheshan Biotechnology Research Co.,Ltd. Address before: 352100 No. 63, Fengyang village, Fengyang village, Yingshan Township, Zherong County, Ningde City, Fujian Province Patentee before: FUJIAN ZHESHEN BIOTECHNOLOGY CO.,LTD. |
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