CN105596257B - Antioxidant activity object, extracting method and the application in cosmetics of shiny-leaved yellowhorn leaf - Google Patents
Antioxidant activity object, extracting method and the application in cosmetics of shiny-leaved yellowhorn leaf Download PDFInfo
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- CN105596257B CN105596257B CN201610034210.1A CN201610034210A CN105596257B CN 105596257 B CN105596257 B CN 105596257B CN 201610034210 A CN201610034210 A CN 201610034210A CN 105596257 B CN105596257 B CN 105596257B
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- shiny
- leaved yellowhorn
- yellowhorn leaf
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
Abstract
The invention belongs to technical field of plant extraction, it is related to a kind of antioxidant activity object of shiny-leaved yellowhorn leaf, extracting method and the application in cosmetics.Solve the prior art technical problem low to shiny-leaved yellowhorn leaf utilization ratio.It includes the following steps：A, fresh shiny-leaved yellowhorn leaf is taken, dry shiny-leaved yellowhorn leaf is obtained after dry；B, the shiny-leaved yellowhorn leaf obtained in step A is extracted with organic solvent, extracting solution is obtained after extract liquor filtering；C, the extracting solution in step B is concentrated to give concentrate, obtains the antioxidant activity object of shiny-leaved yellowhorn leaf after concentrate drying, can be used in cosmetics.The active matter of the present invention has antioxidation, can be used in cosmetics.
Description
Technical field
The invention belongs to technical field of plant extraction, are related to a kind of shiny-leaved yellowhorn more particularly to a kind of antioxygen of shiny-leaved yellowhorn leaf
Change active matter, extracting method and the application in cosmetics.
Background technology
Shiny-leaved yellowhorn (scientific name：Xanthoceras sorbifolium Bunge), Sapindaceae, shiny-leaved yellowhorn category machaka
Or dungarunga, it is high up to 5 meters；Sprig maroon is sturdy, and leaf connecting handle length is up to 30 centimetres；For leaflet to life, both sides are slightly asymmetric, top
Hold tapering, base portion wedge shape, there is sharp keen sawtooth at edge, and the inflorescence basidixed of hermaphrodite flower, male inflorescence axillary, uprightly, total bennet are short, petal
The horn shape appendage of white, base portion aubergine or yellow, floral disc is orange-yellow, and filigree is hairless；Capsule is up to 6 centimetres；Seed black
And it is glossy.Spring blooms, autumn firstfruits.
Shiny-leaved yellowhorn the Northwest originating in China, is mainly distributed on the provinces such as Shaanxi, Shanxi, Hebei, Inner Mongol, there is higher food
With, economic values such as medicinal, ornamental.There are wind-damp dispelling, swelling and pain relieving and other effects, is clinically mainly used for treating rheumatism joint
The diseases such as heat, skin wind-heat, infantile enuresis in scorching, rheumatism, and it has been included in as medicinal part the Chinese Pharmacopoeia of version in 1977.Separately
Outside, shiny-leaved yellowhorn adaptability is very strong, and drought-resistant, barren, well developed root system, sprout tiller strength is strong, grows fast, solid morning, yield height, service life
Length, economic value are big, are the ornamental and important woody oil tree species of preciousness.Shiny-leaved yellowhorn is classified as woody fuel oil by country at present
Energy chief species, Breeding and popularization are paid much attention to by country, and it is imperative to greatly develop shiny-leaved yellowhorn.
The existing shiny-leaved yellowhorn cultivated area in the whole nation is up to 5.35 ten thousand hm2, it is developed and used at present based on being extracted oil with benevolence.Wen Guan
Fruit leaf contain there are many chemical composition (Ma Yangmin, king wear, research [J] Chinese patent drugs of shiny-leaved yellowhorn study on chemical compositions of leaves, 2010,32
(10), 1750-1753) and have the function of anti-inflammatory analgetic, hemostasia and detumescence.Currently, to the utilization of shiny-leaved yellowhorn leaf with tealeaves
Based on, as Chinese patent literature discloses a kind of shiny-leaved yellowhorn leaf tea and its applies [application number：201310217440.8], it is a kind of
Using shiny-leaved yellowhorn leaf as primary raw material, tea [application number is supported using the drink that black tea processing technology makes：201110238087.2], it is a kind of
Tealeaves [the patent No. containing bunkankasaponin bioactive substance:200710018943.7].
Invention content
Regarding the issue above, the present invention provides a kind of antioxidant activity object of shiny-leaved yellowhorn leaf easy to implement carries
Take method.
It is a further object of the present invention to provide a kind of antioxidant activity objects of shiny-leaved yellowhorn leaf.
Another object of the present invention is to provide a kind of antioxidant activity object for the shiny-leaved yellowhorn leaf that can be used for cosmetics.
In order to achieve the above objectives, present invention employs following technical proposals：A kind of antioxidant activity object of shiny-leaved yellowhorn leaf carries
Method is taken, is included the following steps：
A, fresh shiny-leaved yellowhorn leaf is taken, dry shiny-leaved yellowhorn leaf is obtained after dry；
B, the shiny-leaved yellowhorn leaf obtained in step A is extracted with organic solvent, extracting solution is obtained after extract liquor filtering；It is organic molten
Agent can be alcohol, ether, petroleum ether, ethyl acetate of 1-4 carbon atoms etc..
C, the extracting solution in step B is concentrated to give concentrate, the antioxygen of shiny-leaved yellowhorn leaf is obtained after concentrate drying
Change active matter.
In the antioxidant activity object extracting method of above-mentioned shiny-leaved yellowhorn leaf, in step, the drying shiny-leaved yellowhorn leaf
It is made using the method that interior is dried in the shade, in stepb, the organic solvent is ethanol solution, and in step C, concentration uses
It is concentrated in vacuo, it is dry using freeze-drying.
In the antioxidant activity object extracting method of above-mentioned shiny-leaved yellowhorn leaf, in stepb, the ethanol solution is
The ethanol water of 30-95V/V%.
In the antioxidant activity object extracting method of above-mentioned shiny-leaved yellowhorn leaf, in stepb, using the method being heated to reflux
It is extracted, ethanol solution is the ethanol water of 70-95V/V%.
In the antioxidant activity object extracting method of above-mentioned shiny-leaved yellowhorn leaf, in stepb, ethanol solution 95V/V%
Ethanol water.
In the antioxidant activity object extracting method of above-mentioned shiny-leaved yellowhorn leaf, in stepb, the heating time being heated to reflux
For 1-3h, the weight ratio of dry shiny-leaved yellowhorn leaf and organic solvent is 1:1-1.5.
In the antioxidant activity object extracting method of above-mentioned shiny-leaved yellowhorn leaf, extraction times are 1-3 times, and extract liquor merges
After obtain extracting solution.
In the antioxidant activity object extracting method of above-mentioned shiny-leaved yellowhorn leaf, in step, dry shiny-leaved yellowhorn leaf is crushed to
40-60 mesh.
According to the antioxidant activity object of shiny-leaved yellowhorn leaf made from above-mentioned extracting method.
According to application of the antioxidant activity object of shiny-leaved yellowhorn leaf made from above-mentioned extracting method in cosmetics, cosmetics
Can be facial cleanser, facial mask, washing cream etc..
Compared with prior art, the advantage of the invention is that：The resisting from shiny-leaved yellowhorn leaf screened through the invention
Oxidation activity position can be applied to prepare the cosmetics such as facial cleanser, facial mask, washing cream etc. with antioxidant activity.
Description of the drawings
Fig. 1 is the curve graph of active matter and the DPPH clearance rates of VC reference substances that comparative example provides；
Fig. 2 is the curve graph of active matter and the DPPH clearance rates of VC reference substances that embodiment 1 provides；
Fig. 3 is the curve graph of active matter and the DPPH clearance rates of VC reference substances that embodiment 2 provides；
Fig. 4 is the curve graph of active matter and the DPPH clearance rates of VC reference substances that embodiment 3 provides；
Fig. 5 is the curve graph of active matter and the DPPH clearance rates of VC reference substances that embodiment 4 provides.
Specific implementation mode
Embodiment 1
Fresh shiny-leaved yellowhorn leaf 500g is taken, dries in the shade under normal temperature laboratory, be crushed to spare, addition 30% bodies of 500g after 60 mesh
The ethanol water of product ratio, is heated to reflux, and obtains extracting solution after phegma filtering, flows back 3 times, each 3h, flow back feed liquid every time
Than identical, merging extracting solution, extracting solution is concentrated in vacuo, and vacuum degree 0.09MPa is freeze-dried after concentration, obtains shiny-leaved yellowhorn leaf
Antioxidant activity object.
Embodiment 2
Fresh shiny-leaved yellowhorn leaf 500g is taken, dries in the shade under normal temperature laboratory, be crushed to spare, addition 50% bodies of 500g after 60 mesh
The ethanol water of product ratio, is heated to reflux, and obtains extracting solution after phegma filtering, flows back 3 times, each 3h, flow back feed liquid every time
Than identical, merging extracting solution, extracting solution is concentrated in vacuo, and vacuum degree 0.09MPa is freeze-dried after concentration, obtains shiny-leaved yellowhorn leaf
Antioxidant activity object.
Embodiment 3
Fresh shiny-leaved yellowhorn leaf 500g is taken, dries in the shade under normal temperature laboratory, be crushed to spare, addition 70% bodies of 500g after 60 mesh
The ethanol water of product ratio, is heated to reflux, and obtains extracting solution after phegma filtering, flows back 3 times, each 3h, flow back feed liquid every time
Than identical, merging extracting solution, extracting solution is concentrated in vacuo, and vacuum degree 0.09MPa is freeze-dried after concentration, obtains shiny-leaved yellowhorn leaf
Antioxidant activity object.
Embodiment 4
Fresh shiny-leaved yellowhorn leaf 500g is taken, dries in the shade under normal temperature laboratory, be crushed to spare, addition 95% bodies of 500g after 60 mesh
The ethanol water of product ratio, is heated to reflux, and obtains extracting solution after phegma filtering, flows back 3 times, each 3h, flow back feed liquid every time
Than identical, merging extracting solution, extracting solution is concentrated in vacuo, and vacuum degree 0.09MPa is freeze-dried after concentration, obtains shiny-leaved yellowhorn leaf
Antioxidant activity object.
Embodiment 5
Fresh shiny-leaved yellowhorn leaf 500g is taken, dries in the shade under normal temperature laboratory, be crushed to spare, addition 750g acetic acid second after 40 mesh
Ester is heated to reflux, and flow back 1h, and extracting solution is obtained after phegma filtering, and extracting solution is concentrated in vacuo, vacuum degree 0.09MPa, concentration
After be freeze-dried, obtain the antioxidant activity object of shiny-leaved yellowhorn leaf.
Embodiment 6
Fresh shiny-leaved yellowhorn leaf 500g is taken, dries in the shade under normal temperature laboratory, be crushed to spare, addition 600g petroleum ethers after 50 mesh,
It being heated to reflux, obtains extracting solution after phegma filtering, flow back 2 times, each 2h, the solid-liquid ratio that flows back every time is identical, merges extracting solution,
Extracting solution is concentrated in vacuo, and vacuum degree 0.09MPa is freeze-dried after concentration, obtains the antioxidant activity object of shiny-leaved yellowhorn leaf.
Embodiment 7
Fresh shiny-leaved yellowhorn leaf 500g is taken, dries in the shade under normal temperature laboratory, be crushed to spare, addition 600g petroleum ethers after 50 mesh,
It being heated to reflux, obtains extracting solution after phegma filtering, flow back 2 times, each 2h, the solid-liquid ratio that flows back every time is identical, merges extracting solution,
Extracting solution is concentrated in vacuo, and vacuum degree 0.09MPa is freeze-dried after concentration, obtains the antioxidant activity object of shiny-leaved yellowhorn leaf.
Comparative example
Fresh shiny-leaved yellowhorn leaf 500g is taken, dries in the shade under normal temperature laboratory, be crushed to spare, addition 500g pure water after 60 mesh,
It being heated to reflux, obtains extracting solution after phegma filtering, flow back 3 times, each 3h, the solid-liquid ratio that flows back every time is identical, merges extracting solution,
Extracting solution is concentrated in vacuo, and vacuum degree 0.09MPa is freeze-dried after concentration, obtains the antioxidant activity object of shiny-leaved yellowhorn leaf.
External DPPH radicals scavengings experiment
(1) preparation of VC reference substances and sample solution：Precision weighs prepared by VC reference substances, embodiment 1-4 and comparative example
In 25ml volumetric flasks, methanol dissolves and is settled to 25ml each 25mg of antioxidant activity object of shiny-leaved yellowhorn leaf.It takes respectively wherein
2ml is dissolved in 10ml volumetric flasks, and methanol dilution to scale obtains the solution for standby of 0.01mg/ml；
0.04mg/ml, 0.08mg/ml, 0.12mg/ml, 0.16mg/ml, 0.20mg/ are prepared respectively by same procedure again
The reference substance and sample working solution of ml concentration gradients.
(2) preparation of DPPH standard solutions：Precision weighs DPPH standard items 12mg, is placed in 250ml volumetric flasks,
Methanol dissolving and constant volume, obtain DPPH titers, keep in dark place at low temperature spare.
(3) the different extracted parts scavenging activity on DPPH measures：2ml DPPH titers are taken to be prepared by step (1) with 2ml
The VC reference substances of same concentrations value, embodiment 1-4 and comparative example solution mixing, be protected from light 15min, the places 517nm measurement
Absorbance A sample.To 0.01mg/ml, 0.04mg/ml, 0.08mg/ml, 0.12mg/ml, 0.16mg/ml, 0.20mg/ml
Concentration gradient sequentially determining.
DPPH clearance rate computational methods：
DPPH clearance rates=A blank-A samples/A blank × 100%
Wherein blank is the DPPH solution of 4ml
Experimental result is shown：Referring to Fig.1 shown in -5, the anti-oxidant work of the shiny-leaved yellowhorn leaf of embodiment 1-4 and comparative example preparation
Property object have certain scavenging capacity to DPPH free radicals, the active matter that wherein embodiment 4 provides is free to removing DPPH
Base activity is most strong, in a concentration of 0.2mg/ml, to the clearance rates of DPPH free radicals up to 85.78%, and under same concentrations
The clearance rate of VC reference substances is 90.21%.
Application examples
The antioxidant activity object of shiny-leaved yellowhorn leaf prepared by embodiment 1-7 all has antioxidant activity, is applied in cosmetics.
Especially, the antioxidant activity object for the shiny-leaved yellowhorn leaf that embodiment 4 provides has good antioxidant activity, in concentration
For 0.2mg/l when, to the clearance rates of DPPH free radicals up to 85.78%.The active matter derives from natural plant anti-oxidation
Agent, and the extractant used in extraction process is water and ethanol water solvent, it is non-toxic to humans, it is excellent cosmetics addition
Agent is applied to prepare the cosmetics such as facial cleanser, facial mask, washing cream etc. with antioxidant activity.
As for additive amount of the antioxidant activity object in cosmetics of shiny-leaved yellowhorn leaf, meeting relevant industries standard or enterprise
When standard, it can be formulated according to the different type of cosmetics, formula, performance.Also the additive amount that can refer to VC gives
To formulate.
Specific embodiment described herein is only an example for the spirit of the invention.Technology belonging to the present invention is led
The technical staff in domain can make various modifications or additions to the described embodiments or replace by a similar method
In generation, however, it does not deviate from the spirit of the invention or beyond the scope of the appended claims.
Claims (3)
1. a kind of antioxidant activity object extracting method of shiny-leaved yellowhorn leaf, which is characterized in that include the following steps：
A, fresh shiny-leaved yellowhorn leaf is taken, dry shiny-leaved yellowhorn leaf is obtained after dry；
B, the shiny-leaved yellowhorn leaf obtained in step A is extracted with organic solvent, extracting solution is obtained after extract liquor filtering；
C, the extracting solution in step B is concentrated to give concentrate, the anti-oxidant work of shiny-leaved yellowhorn leaf is obtained after concentrate drying
Property object,
In step, the drying shiny-leaved yellowhorn leaf is made using the indoor method dried in the shade, and dry shiny-leaved yellowhorn leaf is crushed to 60
Mesh, in stepb, the organic solvent are ethanol solution, are extracted using the method being heated to reflux, ethanol solution is
The ethanol water of 95V/V%, the heating time being heated to reflux are 3h, and the weight ratio of dry shiny-leaved yellowhorn leaf and organic solvent is 1:
1, extraction times are 3 times, and extract liquor obtains extracting solution after merging, and in step C, concentration is dry using cold using vacuum concentration
It is lyophilized dry.
2. shiny-leaved yellowhorn leaf is anti-oxidant made from the antioxidant activity object extracting method of shiny-leaved yellowhorn leaf according to claim 1
Active matter.
3. antioxidant activity object the answering in cosmetics of shiny-leaved yellowhorn leaf made from extracting method according to claim 1
With.
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| CN101242850A (en) * | 2003-10-09 | 2008-08-13 | 太平洋艾瑞有限公司 | Composition, function and use of xanthoceras sorbifolia extract and compound isolated from same, method for preparing same |
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| CN101242850A (en) * | 2003-10-09 | 2008-08-13 | 太平洋艾瑞有限公司 | Composition, function and use of xanthoceras sorbifolia extract and compound isolated from same, method for preparing same |
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