CN106309261B - A kind of Chinese chestnut flower extract and its application - Google Patents
A kind of Chinese chestnut flower extract and its application Download PDFInfo
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- CN106309261B CN106309261B CN201611040476.3A CN201611040476A CN106309261B CN 106309261 B CN106309261 B CN 106309261B CN 201611040476 A CN201611040476 A CN 201611040476A CN 106309261 B CN106309261 B CN 106309261B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/81—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions involving only carbon-to-carbon unsaturated bonds
- A61K8/8141—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides or nitriles thereof; Compositions of derivatives of such polymers
- A61K8/8147—Homopolymers or copolymers of acids; Metal or ammonium salts thereof, e.g. crotonic acid, (meth)acrylic acid; Compositions of derivatives of such polymers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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Abstract
The invention discloses a kind of Chinese chestnut flower extract and its applications.The present invention protects a kind of preparation method of Chinese chestnut flower extract first; include the following steps: that the water by chestnut flower male flower and 1 mass parts that fresh weight is 1 mass parts is added in reaction kettle; 110 DEG C of circulation distillations under conditions of steam pressure 0.1Mpa; circulation starts persistent collection distillate after 3 hours of distillation; distillate carries out water-oil separating and collects water phase; 1 mass parts water phase, as Chinese chestnut flower extract are obtained until collecting；The kind of the Chinese chestnut is " Yanshan Mountain is early rich ".The present invention also protects a kind of nutrient solution for skin care, including the Chinese chestnut flower extract.In the case where Chinese Chestnut does not have industrial value, the largely status that wastes, the present inventor is used for extract preparation, and pass through a large amount of process parameter optimizings, a kind of extract that may be used as skin care item and work well is obtained, there is very great value for world chestnut industry, there is the huge market space.
Description
Technical field
The present invention relates to a kind of Chinese chestnut flower extract and its applications.
Background technique
Chestnut (Castanea mollissima Bl.), nickname Chinese chestnut are Dicotyledoneae Fagales Fagaceae Castaneas
The general name of chestnut kind plant.See " Book of Songs " book earliest in ancient book, it is known that the cultivation history of chestnut is in China at least 2,500
The history in remaining year.The botanical character of Chinese chestnut: up to 20 meters of arbor, 80 centimetres of the diameter of a cross-section of a tree trunk 1.3 meters above the ground, hibernaculum is about 5 millimeters, and sprig ash is brown
Color, stipule oblong is 10-15 millimeters long, is become mildewed and squama gland by dredging；Elliptic leaf is to oblong, 11-17 centimetres long, wide dilute up to 7 lis
Rice, petiole are 1-2 centimetres long；Male inflorescence is 10-20 centimetres long, rachis coat, spends 3-5 consor cluster, female flower 1-3 (- 5) hair
Educate solid, style lower part coat；Nut is 1.5-3 centimetres high, 1.8-3.5 centimetres wide；The month at florescence 4-6, the fruiting period 8-10 month.Chinese medicine is recognized
There is kidney and spleen invigorating, physique enhancing and bone strengthening, beneficial stomach calming the liver and other effects for Chinese chestnut, therefore chestnut has the good name of " fruit of kidney " again.Chinese chestnut makees
It is with a long history for the fruit tree of Chinese tradition, it is resourceful, it is widely distributed, it is important exported product.The Chinese chestnut plantation of China
Area is distributed in the ground such as Liaoning, Beijing, Hebei, Shandong, Henan, Shaanxi Huanglong County.
The variety type of Chinese chestnut up to more than 300 or more.By its distributed areas, two big Breeds can be substantially divided into,
I.e. northern Breeds (North China local varieties group) and southern Breeds (Yangtze river basin local varieties group).It is divided from geographic area,
Chinese chestnut is broadly divided into: 1. Qianxi Chinese chestnut (Chinese chestnut that Yanshan mountain range is produced to the east of Beijing, main producing region in Xinglong, Hebei, Zunhua, move
A western band, also known as " Yanshan Mountain Chinese chestnut ", " prosperous Chinese chestnut " etc.)；2. Dandong Chinese chestnut (originates in Dandong City, Liaoning Province)；3. Zhenan County Chinese chestnut
(originating in Shan south Qin_Ba mountain areas)；4. Luotian Chinese chestnut (originates in the Luotian E Dong)；5. Tancheng Chinese chestnut (originates in Shandong Province Tancheng County)；6. believing
Positive Chinese chestnut (originating in Xinyang City, Henan Province)；7. Shao shop Chinese chestnut (originating in Jiangsu Shao Dian).Existing Cultivars of Chinese Chestnut mainly has: 1. magnificent
It is rich: Shandong Fruit-tree Inst.'s breeding from the filial generation of wild miscellaneous 12 (wild Chinese chestnut × Chinese chestnut) × Chinese chestnut；2. Yanshan Mountain chief's chestnut: river
The Qianxi County Bei Sheng is selected from chestnut of growing directly from seeds, and primary number is 107；3. red chestnut 1: Shandong Fruit-tree Inst. is from Hong Li × Tai'an
It is screened in shell filial generation；4. the Shandong Fruit-tree Inst. of Tancheng 3 selects from chestnut of growing directly from seeds；5. stone is rich: Shandong Province sea
Yang Xian is selected from the chestnut that grows directly from seeds；6. feux rouges chestnut: Shandong Province, the township Dian Bu, Laixi City is selected from chestnut of growing directly from seeds；7. the Yanshan Mountain is early rich: river
Northern province's agricultural and forest science institute Changli fruit tree research is selected from the Qianxi County village Yang Jiayu seedling tree；8. swallow Kui: Hebei province Changli fruit
Tree reason, which is grown directly from seeds, to be selected in chestnut；9. silver-colored rich: Beijing woods fruit is selected from the chestnut that grows directly from seeds；10. sharp item oil chestnut: Jiangsu Province plant institute
It is selected from the chestnut that grows directly from seeds.The market of world chestnut industry focuses primarily upon food, i.e. Fruits in Chinese Chestnut and its related processed food at present.It is right
For Chinese chestnut plant, fruit is its seldom a part, if it is possible to research and develop, open to the other parts of Chinese chestnut plant
The industrial value for sending out other parts, will generate huge economic benefit for world chestnut industry.
Summary of the invention
The object of the present invention is to provide a kind of Chinese chestnut flower extract and its applications.
The present invention protects a kind of preparation method of Chinese chestnut flower extract first, includes the following steps:
The water of chestnut flower male flower and 1 mass parts that fresh weight is 1 mass parts is added in reaction kettle, in steam pressure 0.1Mpa
Under conditions of the distillation of 110 DEG C of circulations, start persistent collection distillate after circulation 3 hours of distillation, distillate carries out water-oil separating
And water phase is collected, 1 mass parts water phase, as Chinese chestnut flower extract are obtained until collecting；The kind of the Chinese chestnut is that " Yanshan Mountain is early
It is rich ".
The temperature for the cooling water that " the collecting distillate " uses is 20-25 DEG C, and concretely 25 DEG C.
The chestnut flower is proposed with chestnut flower within picking 24 hours.
The chestnut flower be proposed with picking 7 days in and always 4 DEG C refrigeration chestnut flower.
The Chinese chestnut flower extract that any description above method is prepared also belongs to protection scope of the present invention.
The present invention also protects a kind of nutrient solution for skin care, including any description above Chinese chestnut flower extract.
The nutrient solution further includes following ingredient: Sodium Hyaluronate, 1,3-PD, carbomer SF-1, D-pantothenyl aleohol, Xin Gan
Pure and mild Sensiva SC50.In the nutrient solution, Chinese chestnut flower extract, Sodium Hyaluronate, 1,3-PD, carbomer SF-1,
The quality proportioning of D-pantothenyl aleohol, pungent glycol and Sensiva SC50 is as follows: 88.9:2.0:4.0:0.5:1.5:1.5:1.5.The battalion
Nutrient solution can be by Chinese chestnut flower extract, Sodium Hyaluronate, 1,3-PD, carbomer SF-1, D-pantothenyl aleohol, pungent glycol and ethylhexyl
Glycerol composition.
The nutrient solution further includes following ingredient: Sodium Hyaluronate, 1,3-PD, carbomer SF-1, D-pantothenyl aleohol, Xin Gan
Alcohol, Sensiva SC50 and essence (essence can arbitrarily be chosen, such as flavoring rose essence as needed for adjusting fragrance).It is described
In nutrient solution, Chinese chestnut flower extract, Sodium Hyaluronate, 1,3-PD, carbomer SF-1, D-pantothenyl aleohol, pungent glycol, ethylhexyl
The quality proportioning of glycerol and essence is as follows: 88.9:2.0:4.0:0.5:1.5:1.5:1.5:0.1.The nutrient solution can be by Chinese chestnut
Flower extract, Sodium Hyaluronate, 1,3-PD, carbomer SF-1, D-pantothenyl aleohol, pungent glycol, Sensiva SC50 and essence group
At.
The present invention also protects a kind of facial mask, be loaded on face mask substrate material any description above nutrient solution obtain it is (specific
For, every face mask substrate material can load 15-25ml nutrient solution).
In the case where Chinese Chestnut does not have industrial value, the largely status that wastes, the present inventor is used for extract
Preparation, and by a large amount of process parameter optimizings, a kind of extract that may be used as skin care item and work well is obtained, for plate
Chestnut industry has very great value, has the huge market space.
Specific embodiment
Embodiment below facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method is unless otherwise specified conventional method.Test material as used in the following examples is unless otherwise specified certainly
What routine biochemistry reagent shop was commercially available.Quantitative test in following embodiment is respectively provided with three repeated experiments, as a result makes even
Mean value.
Sodium Hyaluronate: No. CAS: 9067-32-7.D-pantothenyl aleohol: No. CAS: 81-13-0.Sensiva SC50: CAS:
70445-33-9.Carbomer SF-1 (English name: Carbomer SF-1): the joyful beauty Biotechnology Co., Ltd in Wuhan.Rose
Essence: Shanghai Yi Kesaiting fragrance Co., Ltd.Face mask substrate material in embodiment is Huizhou City's U.S.A, that is, limited public affairs of bonded fabric product
Department, article No. are the silk invisible face pack paper of MG 813029.
Embodiment 1, the preparation of Chinese chestnut flower extract and the preparation of reference material
One, the preparation of Chinese chestnut flower extract
In by the end of May, 2016 at the beginning of 6 months, 5 points to 9 points of every morning, from the Chestnut Orchard of Hebei province Qianxi County, (Cultivars of Chinese Chestnut is
" Yanshan Mountain is early rich ") in acquire chestnut flower male flower.
It is that 300kg chestnut flower male flower (within picking 24 hours) and 300kg water are added in reaction kettle by fresh weight, in vapour pressure
110 DEG C of circulation distillations under conditions of power 0.1Mpa, circulation start persistent collection distillate (cooling water temperature after 3 hours of distillation
It is 25 DEG C), distillate carries out water-oil separating and collects water phase, obtains 300kg water phase, as Chinese chestnut flower extract until collecting.
It collects distillate and distillate progress water-oil separating is that continuous linking carries out.
The production for carrying out five batches, obtained Chinese chestnut flower extract is successively named as Chinese chestnut flower extract I, chestnut flower mentions
Take object II, Chinese chestnut flower extract III, Chinese chestnut flower extract IV and Chinese chestnut flower extract V.
Two, the component parsing of Chinese chestnut flower extract
The Chinese chestnut flower extract for taking step 1 to prepare, using gas chromatography-mass spectrography (Agilent SB-308) to wherein
Organic matter carry out component resolving.
Organic matter in Chinese chestnut flower extract I is counted as 100%, wherein (% is that quality percentage contains containing following substance
Amount):
In organic matter in organic matter in Chinese chestnut flower extract II, in Chinese chestnut flower extract III, Chinese chestnut flower extract IV
In organic matter in and Chinese chestnut flower extract V in organic matter in, the content of above-mentioned each substance and its in Chinese chestnut flower extract
The content in organic matter in I is not significantly different.
Three, the preparation of reference material A
In by the end of May, 2016 at the beginning of 6 months, 5 points to 9 points of every morning, from the Chestnut Orchard of Hebei province Qianxi County, (Cultivars of Chinese Chestnut is
" Yanshan Mountain is early rich ") in acquire chestnut flower male flower.
1, the preparation of reference material A- I
It is that 300kg chestnut flower male flower (within picking 24 hours) and 400kg water are added in reaction kettle by fresh weight, in vapour pressure
110 DEG C of circulation distillations under conditions of power 0.1Mpa, circulation start persistent collection distillate (cooling water temperature after 3 hours of distillation
It is 25 DEG C), distillate carries out water-oil separating and collects water phase, obtains 350kg water phase, as reference material A- I until collecting.It carries out
The production of two batches, obtained reference material A- I are successively named as reference material A- I -1, reference material A- I -2.
2, the preparation of reference material A- II
It is that 300kg chestnut flower male flower (within picking 24 hours) and 300kg water are added in reaction kettle by fresh weight, in vapour pressure
110 DEG C of circulation distillations under conditions of power 0.2Mpa, circulation start persistent collection distillate (cooling water temperature after 3 hours of distillation
It is 25 DEG C), distillate carries out water-oil separating and collects water phase, obtains 300kg water phase, as reference material A- II until collecting.Into
The production of two batches of row, obtained reference material A- II are successively named as reference material A- II -1, reference material A- II -2.
3, the preparation of reference material A- III
It is that 300kg chestnut flower male flower (within picking 24 hours) and 300kg water are added in reaction kettle by fresh weight, in vapour pressure
105 DEG C of circulation distillations under conditions of power 0.1Mpa, circulation start persistent collection distillate (cooling water temperature after 3 hours of distillation
It is 25 DEG C), distillate carries out water-oil separating and collects water phase, obtains 300kg water phase, as reference material A- III until collecting.Into
The production of two batches of row, obtained reference material A- III are successively named as reference material A- III -1, reference material A- III -2.
4, the preparation of reference material A- IV
It is that 300kg chestnut flower male flower (within picking 24 hours) and 300kg water are added in reaction kettle by fresh weight, in vapour pressure
110 DEG C of circulation distillations under conditions of power 0.1Mpa, circulation start persistent collection distillate (cooling water temperature after 2 hours of distillation
It is 25 DEG C), distillate carries out water-oil separating and collects water phase, obtains 300kg water phase, as reference material A- IV until collecting.Into
The production of two batches of row, obtained reference material A- IV are successively named as reference material A- IV -1, reference material A- IV -2.
5, the preparation of reference material A- V
It is that 300kg chestnut flower male flower (within picking 24 hours) and 300kg water are added in reaction kettle by fresh weight, in vapour pressure
110 DEG C of circulation distillations under conditions of power 0.1Mpa, circulation start persistent collection distillate (cooling water temperature after 3 hours of distillation
It is 25 DEG C), distillate carries out water-oil separating and collects water phase, obtains 200kg water phase, as reference material A- V until collecting.Into
The production of two batches of row, obtained reference material A- V are successively named as reference material A- V -1, reference material A- V -2.
6, the preparation of reference material A- VI
It is that 300kg chestnut flower male flower (within picking 24 hours) and 300kg water are added in reaction kettle by fresh weight, in vapour pressure
110 DEG C of circulation distillations under conditions of power 0.1Mpa, circulation start persistent collection distillate (cooling water temperature after 3 hours of distillation
It is 30 DEG C), distillate carries out water-oil separating and collects water phase, obtains 300kg water phase, as reference material A- VI until collecting.Into
The production of two batches of row, obtained reference material A- VI are successively named as reference material A- VI -1, reference material A- VI -2.
Four, the preparation of reference material B
In by the end of May, 2016 at the beginning of 6 months, 5 points to 9 points of every morning, from the Chestnut Orchard of Hebei province Qianxi County, (Cultivars of Chinese Chestnut is
" Yanshan Mountain chief's chestnut ") in acquire chestnut flower male flower.
It is that 300kg chestnut flower male flower (within picking 24 hours) and 300kg water are added in reaction kettle by fresh weight, in vapour pressure
110 DEG C of circulation distillations under conditions of power 0.1Mpa, circulation start persistent collection distillate (cooling water temperature after 3 hours of distillation
It is 25 DEG C), distillate carries out water-oil separating and collects water phase, obtains 300kg water phase, as reference material B until collecting.Carry out two
The production of a batch, obtained reference material B are successively named as reference material B-1, reference material B-2.
The preparation of embodiment 2, nutrient solution
Following each raw material is mixed into (% representation quality percentage composition):
Chinese chestnut flower extract prepared by embodiment 1: 88.9%
Sodium Hyaluronate: 2.0%
1,3-PD: 4.0%
Carbomer SF-1:0.5%
D-pantothenyl aleohol: 1.5%
Pungent glycol: 1.5%
Sensiva SC50: 1.5%
Flavoring rose essence: 0.1%.
Nutrient solution prepared by Chinese chestnut flower extract I is named as nutrient solution I.
Nutrient solution prepared by Chinese chestnut flower extract II is named as nutrient solution II.
Nutrient solution prepared by Chinese chestnut flower extract III is named as nutrient solution III.
Nutrient solution prepared by Chinese chestnut flower extract IV is named as nutrient solution IV.
Nutrient solution prepared by Chinese chestnut flower extract V is named as nutrient solution V.
The preparation of embodiment 3, facial mask
Face mask substrate material is taken, nutrient solution (every face mask substrate material loads 15-25ml nutrient solution) prepared by embodiment 2 is added dropwise, obtains
To facial mask.
The facial mask that nutrient solution I obtains is added dropwise and is named as facial mask I.
The facial mask that nutrient solution II obtains is added dropwise and is named as facial mask II.
The facial mask that nutrient solution III obtains is added dropwise and is named as facial mask III.
The facial mask that nutrient solution IV obtains is added dropwise and is named as facial mask IV.
The facial mask that nutrient solution V obtains is added dropwise and is named as facial mask V.
The detection of harmful substance in embodiment 4, each product
Determinand are as follows: the nutrient solution I to V or real of Chinese chestnut flower extract I to V, the preparation of embodiment 2 prepared by embodiment 1
Apply the facial mask I to V of the preparation of example 3.
1, according in cosmetics health specification (version in 2007) correlation technique detection determinand in antiseptic content and
Hormone-content.The result shows that each determinand does not contain following various preservatives: the bromo- 2- nitre of methylchloroisothiazandnone, 2-
Base propane -1,3- glycol, methylisothiazolinone, benzyl alcohol, Phenoxyethanol, 4-HBA ester, benzoic acid, 4- hydroxy benzenes
Ethyl formate, 4-HBA isopropyl ester, 4-HBA propyl ester, 4-HBA isobutyl ester, 4-HBA
Butyl ester.The result shows that each determinand does not contain following various hormones: oestrone, estriol, diethylstilbestrol, estradiol, testis
Ketone, Methyltestosterone, progesterone.
2, permitted [2011] No. 96 attachmentes 1 " detection method of acrylamide in cosmetics " according to state's food medicine prison and detect determinand
In acrylamide content.The result shows that each determinand does not contain acrylamide.
3, according in GB/T5009.78-2003 " analysis method of base paper for foodstuff packaging sanitary standard " detection determinand
Fluorescent material content.The result shows that each determinand does not contain fluorescent material.
The effect detection of embodiment 5, Chinese chestnut flower extract
One, one is tested
Chinese chestnut flower extract I, Chinese chestnut flower extract II, the chestnut flower that Chinese chestnut flower extract refers to prepared by embodiment 1 mention
Object III, Chinese chestnut flower extract IV or Chinese chestnut flower extract V are taken, is tested respectively.
1, a potato is taken, one slices into halves, and takes half, and one of trace is cut out among cross section as line of demarcation.
2, half of potato for taking step 1, the 1ml Chinese chestnut flower extract of smearing on one side in line of demarcation, another side smear 1ml without
Then bacterium water is used the wind of hair dryer high fever 5 minutes, is then observed as control.Smear the not assorted on one side of Chinese chestnut flower extract
Variation.There is fold drying on one side in that smears sterile water, or even white particles occurs.The Chinese chestnut flower extract of each batch
It carries out five repetitions to test, as a result unanimously.The result shows that each Chinese chestnut flower extract all has water lock moistening effect.
3, half of potato for taking step 1, the 1ml Chinese chestnut flower extract of smearing on one side in line of demarcation, another side smear 1ml without
Then bacterium water is respectively smeared 5% tincture of iodine of 1ml on both sides, is observed after five minutes as control.Being oxidized on one side for sterile water is smeared,
Atropurpureus is presented, cannot be wiped with face tissue.The one side for smearing Chinese chestnut flower extract, is presented the brown color of the tincture of iodine, uses face-cloth
Paper can wipe.The Chinese chestnut flower extract of each batch carries out five repetitions and tests, as a result unanimously.The result shows that each plate
Chestnut flower extract all has antioxidant effect.
Two, two are tested
Under the premise of informed consent, 1400 volunteers are recruited, as subject.
Before test, subject cleans the inside of both hands and two forearms with clear water.On the inside of left forearm and on the inside of right forearm
A test area (5 × 5cm) is respectively marked, the test area on the inside of left forearm is named as left area, the trial zone on the inside of right forearm
Domain is named as right area.
First group of subject (100): first contained with the moisture of skin that moisture of skin tester measures Zuo Qu and right area respectively
Measure (MMV0), Chinese chestnut flower extract I prepared by 1ml embodiment 1 then is smeared in left area and smears 1ml embodiment 1 in right area
The reference material A- I -1 of preparation, measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
Second group of subject (100): first contained with the moisture of skin that moisture of skin tester measures Zuo Qu and right area respectively
Measure (MMV0), Chinese chestnut flower extract I prepared by 1ml embodiment 1 then is smeared in left area and smears 1ml embodiment 1 in right area
The reference material A- I -2 of preparation, measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
Third group subject (100): first contained with the moisture of skin that moisture of skin tester measures Zuo Qu and right area respectively
Measure (MMV0), Chinese chestnut flower extract II prepared by 1ml embodiment 1 then is smeared in left area and smears 1ml embodiment 1 in right area
The reference material A- II -1 of preparation, measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
4th group of subject (100): first contained with the moisture of skin that moisture of skin tester measures Zuo Qu and right area respectively
Measure (MMV0), Chinese chestnut flower extract II prepared by 1ml embodiment 1 then is smeared in left area and smears 1ml embodiment 1 in right area
The reference material A- II -2 of preparation, measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
5th group of subject (100): first contained with the moisture of skin that moisture of skin tester measures Zuo Qu and right area respectively
Measure (MMV0), Chinese chestnut flower extract III prepared by 1ml embodiment 1 then is smeared in left area and smears 1ml embodiment 1 in right area
The reference material A- III -1 of preparation, measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
6th group of subject (100): first contained with the moisture of skin that moisture of skin tester measures Zuo Qu and right area respectively
Measure (MMV0), Chinese chestnut flower extract III prepared by 1ml embodiment 1 then is smeared in left area and smears 1ml embodiment 1 in right area
The reference material A- III -2 of preparation, measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
7th group of subject (100): first contained with the moisture of skin that moisture of skin tester measures Zuo Qu and right area respectively
Measure (MMV0), Chinese chestnut flower extract IV prepared by 1ml embodiment 1 then is smeared in left area and smears 1ml embodiment 1 in right area
The reference material A- IV -1 of preparation, measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
8th group of subject (100): first contained with the moisture of skin that moisture of skin tester measures Zuo Qu and right area respectively
Measure (MMV0), Chinese chestnut flower extract IV prepared by 1ml embodiment 1 then is smeared in left area and smears 1ml embodiment 1 in right area
The reference material A- IV -2 of preparation, measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
9th group of subject (100): first contained with the moisture of skin that moisture of skin tester measures Zuo Qu and right area respectively
Measure (MMV0), Chinese chestnut flower extract V prepared by 1ml embodiment 1 then is smeared in left area and smears 1ml embodiment 1 in right area
The reference material A- V -1 of preparation, measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
Tenth group of subject (100): first contained with the moisture of skin that moisture of skin tester measures Zuo Qu and right area respectively
Measure (MMV0), Chinese chestnut flower extract V prepared by 1ml embodiment 1 then is smeared in left area and smears 1ml embodiment 1 in right area
The reference material A- V -2 of preparation, measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
11st group of subject (100): the moisture of skin of Zuo Qu and right area are first measured respectively with moisture of skin tester
Content (MMV0), Chinese chestnut flower extract I prepared by 1ml embodiment 1 then is smeared in left area and smears 1ml embodiment in right area
The reference material A- VI -1 of 1 preparation, measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
12nd group of subject (100): the moisture of skin of Zuo Qu and right area are first measured respectively with moisture of skin tester
Content (MMV0), Chinese chestnut flower extract I prepared by 1ml embodiment 1 then is smeared in left area and smears 1ml embodiment in right area
The reference material A- VI -2 of 1 preparation, measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
13rd group of subject (100): the moisture of skin of Zuo Qu and right area are first measured respectively with moisture of skin tester
Content (MMV0), Chinese chestnut flower extract I prepared by 1ml embodiment 1 then is smeared in left area and smears 1ml embodiment in right area
The reference material B-1 of 1 preparation, measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
14th group of subject (100): the moisture of skin of Zuo Qu and right area are first measured respectively with moisture of skin tester
Content (MMV0), Chinese chestnut flower extract I prepared by 1ml embodiment 1 then is smeared in left area and smears 1ml embodiment in right area
The reference material B-2 of 1 preparation, measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
Skin moisture content growth rate=(MMVt-MMV0)/MMV0× 100%.
As a result the average value in the left area of each group, the average value in the area Huo Quyou are taken.
In first group, the left area's skin moisture content growth rate for smearing Chinese chestnut flower extract I is 40%, smears reference material A-
I -1 right area's skin moisture content growth rate is 15%.
In second group, the left area's skin moisture content growth rate for smearing Chinese chestnut flower extract I is 42%, smears reference material A-
I -2 right area's skin moisture content growth rate is 16%.
In third group, the left area's skin moisture content growth rate for smearing Chinese chestnut flower extract II is 41%, smears reference material
Right area's skin moisture content growth rate of A- II -1 is 13%.
In 4th group, the left area's skin moisture content growth rate for smearing Chinese chestnut flower extract II is 40%, smears reference material
Right area's skin moisture content growth rate of A- II -2 is 12%.
In 5th group, the left area's skin moisture content growth rate for smearing Chinese chestnut flower extract III is 41%, smears reference material
Right area's skin moisture content growth rate of A- III -1 is 15%.
In 6th group, the left area's skin moisture content growth rate for smearing Chinese chestnut flower extract III is 42%, smears reference material
Right area's skin moisture content growth rate of A- III -2 is 14%.
In 7th group, the left area's skin moisture content growth rate for smearing Chinese chestnut flower extract IV is 43%, smears reference material
Right area's skin moisture content growth rate of A- IV -1 is 14%.
In 8th group, the left area's skin moisture content growth rate for smearing Chinese chestnut flower extract IV is 41%, smears reference material
Right area's skin moisture content growth rate of A- IV -2 is 16%.
In 9th group, the left area's skin moisture content growth rate for smearing Chinese chestnut flower extract V is 42%, smears reference material
Right area's skin moisture content growth rate of A- V -1 is 12%.
In tenth group, the left area's skin moisture content growth rate for smearing Chinese chestnut flower extract V is 41%, smears reference material
Right area's skin moisture content growth rate of A- V -2 is 11%.
In 11st group, the left area's skin moisture content growth rate for smearing Chinese chestnut flower extract I is 43%, smears reference material
Right area's skin moisture content growth rate of A- VI -1 is 13%.
In 12nd group, the left area's skin moisture content growth rate for smearing Chinese chestnut flower extract I is 42%, smears reference material
Right area's skin moisture content growth rate of A- VI -2 is 10%.
In 13rd group, the left area's skin moisture content growth rate for smearing Chinese chestnut flower extract I is 41%, smears reference material
Right area's skin moisture content growth rate of B-1 is 13%.
In 14th group, the left area's skin moisture content growth rate for smearing Chinese chestnut flower extract I is 40%, smears reference material
Right area's skin moisture content growth rate of B-2 is 15%.
Three, three are tested
Under the premise of informed consent, 100 volunteers are recruited, as subject, are divided into five groups.
First group of subject (20): the Chinese chestnut flower extract I prepared after daily morning and evening face cleaning using embodiment 1, continuously
Using 30 days, without generation facial anaphylaxis redness phenomenon, and reflection skin-tightening was flexible, moisturizing repairing effect is obvious.
Second group of subject (20): the Chinese chestnut flower extract II prepared after daily morning and evening face cleaning using embodiment 1, continuously
Using 30 days, without generation facial anaphylaxis redness phenomenon, and reflection skin-tightening was flexible, moisturizing repairing effect is obvious.
Third group subject (20): the Chinese chestnut flower extract III prepared after daily morning and evening face cleaning using embodiment 1, continuously
Using 30 days, without generation facial anaphylaxis redness phenomenon, and reflection skin-tightening was flexible, moisturizing repairing effect is obvious.
4th group of subject (20): the Chinese chestnut flower extract IV prepared after daily morning and evening face cleaning using embodiment 1, continuously
Using 30 days, without generation facial anaphylaxis redness phenomenon, and reflection skin-tightening was flexible, moisturizing repairing effect is obvious.
5th group of subject (20): the Chinese chestnut flower extract V prepared after daily morning and evening face cleaning using embodiment 1, continuously
Using 30 days, without generation facial anaphylaxis redness phenomenon, and reflection skin-tightening was flexible, moisturizing repairing effect is obvious.
Embodiment 6, the effect detection of nutrient solution
One, one is tested
Nutrient solution refers to nutrient solution I, nutrient solution II, nutrient solution III, nutrient solution IV or nutrient solution prepared by embodiment 2
V, it is tested respectively.
1, a potato is taken, one slices into halves, and takes half, and one of trace is cut out among cross section as line of demarcation.
2, half of potato for taking step 1, the 1ml nutrient solution of smearing on one side in line of demarcation, another side are smeared 1ml sterile water and are made
For control, then uses the wind of hair dryer high fever 5 minutes, then observe.Smear changing on one side without what for nutrient solution.Smear nothing
There is fold drying on one side in bacterium water, or even white particles occurs.Every kind of nutrient solution carries out five repetitions and tests, and as a result one
It causes.The result shows that each nutrient solution all has water lock moistening effect.
3, half of potato for taking step 1, the 1ml nutrient solution of smearing on one side in line of demarcation, another side are smeared 1ml sterile water and are made
For control, 5% tincture of iodine of 1ml is respectively then smeared on both sides, is observed after five minutes.Being oxidized on one side for sterile water is smeared, is presented purple
Black cannot be wiped with face tissue.The one side for smearing nutrient solution, is presented the brown color of the tincture of iodine, can be wiped with face tissue.
Every kind of nutrient solution carries out five repetitions and tests, as a result unanimously.The result shows that each nutrient solution all has antioxidant effect.
Two, two are tested
Under the premise of informed consent, 500 volunteers are recruited, as subject.
Before test, subject cleans the inside of both hands and two forearms with clear water.On the inside of left forearm and on the inside of right forearm
A test area (5 × 5cm) is respectively marked, the test area on the inside of left forearm is named as left area, the trial zone on the inside of right forearm
Domain is named as right area.
First group of subject (100): first contained with the moisture of skin that moisture of skin tester measures Zuo Qu and right area respectively
Measure (MMV0), nutrient solution I prepared by 1ml embodiment 2 then is smeared in left area and smears prepared by 1ml embodiment 1 in right area
Chinese chestnut flower extract I measures the skin moisture content (MMV in Zuo Qu and right area respectively with moisture of skin tester after 30 minutest)。
Second group of subject (100): first contained with the moisture of skin that moisture of skin tester measures Zuo Qu and right area respectively
Measure (MMV0), nutrient solution II prepared by 1ml embodiment 2 then is smeared in left area and smears prepared by 1ml embodiment 1 in right area
Chinese chestnut flower extract II measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
Third group subject (100): first contained with the moisture of skin that moisture of skin tester measures Zuo Qu and right area respectively
Measure (MMV0), nutrient solution III prepared by 1ml embodiment 2 then is smeared in left area and smears prepared by 1ml embodiment 1 in right area
Chinese chestnut flower extract III measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
4th group of subject (100): first contained with the moisture of skin that moisture of skin tester measures Zuo Qu and right area respectively
Measure (MMV0), nutrient solution IV prepared by 1ml embodiment 2 then is smeared in left area and smears prepared by 1ml embodiment 1 in right area
Chinese chestnut flower extract IV measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
5th group of subject (100): first contained with the moisture of skin that moisture of skin tester measures Zuo Qu and right area respectively
Measure (MMV0), nutrient solution V prepared by 1ml embodiment 2 then is smeared in left area and smears prepared by 1ml embodiment 1 in right area
Chinese chestnut flower extract V measures the skin moisture content of Zuo Qu and right area respectively with moisture of skin tester after 30 minutes
(MMVt)。
Skin moisture content growth rate=(MMVt-MMV0)/MMV0× 100%.
As a result the average value in the left area of each group, the average value in the area Huo Quyou are taken.
In first group, the left area's skin moisture content growth rate for smearing nutrient solution I is 48%, smears Chinese chestnut flower extract I
Right area's skin moisture content growth rate be 41%.
In second group, the left area's skin moisture content growth rate for smearing nutrient solution II is 49%, smears Chinese chestnut flower extract
II right area's skin moisture content growth rate is 40%.
In third group, the left area's skin moisture content growth rate for smearing nutrient solution III is 48%, smears Chinese chestnut flower extract
III right area's skin moisture content growth rate is 42%.
In 4th group, the left area's skin moisture content growth rate for smearing nutrient solution IV is 47%, smears Chinese chestnut flower extract
IV right area's skin moisture content growth rate is 40%.
In 5th group, the left area's skin moisture content growth rate for smearing nutrient solution V is 49%, smears Chinese chestnut flower extract
V right area's skin moisture content growth rate is 41%.
Three, three are tested
Under the premise of informed consent, 100 volunteers are recruited, as subject, are divided into five groups.
First group of subject (20): the nutrient solution I prepared after daily morning and evening face cleaning using embodiment 2, continuous use 30
It, without generation facial anaphylaxis redness phenomenon, and reflection skin-tightening is flexible, moisturizing repairing effect is obvious.
Second group of subject (20): the nutrient solution II prepared after daily morning and evening face cleaning using embodiment 2, continuous use 30
It, without generation facial anaphylaxis redness phenomenon, and reflection skin-tightening is flexible, moisturizing repairing effect is obvious.
Third group subject (20): the nutrient solution III prepared after daily morning and evening face cleaning using embodiment 2, continuous use 30
It, without generation facial anaphylaxis redness phenomenon, and reflection skin-tightening is flexible, moisturizing repairing effect is obvious.
4th group of subject (20): the nutrient solution IV prepared after daily morning and evening face cleaning using embodiment 2, continuous use 30
It, without generation facial anaphylaxis redness phenomenon, and reflection skin-tightening is flexible, moisturizing repairing effect is obvious.
5th group of subject (20): the nutrient solution V prepared after daily morning and evening face cleaning using embodiment 2, continuous use 30
It, without generation facial anaphylaxis redness phenomenon, and reflection skin-tightening is flexible, moisturizing repairing effect is obvious.
Embodiment 7, the effect detection of facial mask
Under the premise of informed consent, 100 volunteers are recruited, as subject, are divided into five groups.
First group of subject (20): facial mask I prepared with embodiment 3 is made (to stick facial 15-20 points before sleeping every night
Then clock is washed one's face with clear water), it is used continuously 30 days, without generation facial anaphylaxis redness phenomenon, and reflection skin-tightening has bullet
Property, moisturizing repairing effect are obvious.
Second group of subject (20): facial mask II prepared with embodiment 3 is made (to stick facial 15-20 before sleeping every night
Minute, then washed one's face with clear water), it is used continuously 30 days, without generation facial anaphylaxis redness phenomenon, and reflection skin-tightening has
Elasticity, moisturizing repairing effect are obvious.
Third group subject (20): facial mask III prepared with embodiment 3 is made (to stick facial 15-20 before sleeping every night
Minute, then washed one's face with clear water), it is used continuously 30 days, without generation facial anaphylaxis redness phenomenon, and reflection skin-tightening has
Elasticity, moisturizing repairing effect are obvious.
4th group of subject (20): facial mask IV prepared with embodiment 3 is made (to stick facial 15-20 before sleeping every night
Minute, then washed one's face with clear water), it is used continuously 30 days, without generation facial anaphylaxis redness phenomenon, and reflection skin-tightening has
Elasticity, moisturizing repairing effect are obvious.
5th group of subject (20): facial mask V prepared with embodiment 3 is made (to stick facial 15-20 before sleeping every night
Minute, then washed one's face with clear water), it is used continuously 30 days, without generation facial anaphylaxis redness phenomenon, and reflection skin-tightening has
Elasticity, moisturizing repairing effect are obvious.
Claims (8)
1. a kind of preparation method of Chinese chestnut flower extract includes the following steps: the chestnut flower male flower and 1 by fresh weight for 1 mass parts
The water of mass parts is added in reaction kettle, 110 DEG C of circulation distillations under conditions of steam pressure 0.1Mpa, circulation 3 hours of distillation
After start persistent collection distillate, distillate carries out water-oil separating and simultaneously collects water phase, obtains 1 mass parts water phase until collecting, i.e.,
For Chinese chestnut flower extract；The kind of the Chinese chestnut is " Yanshan Mountain is early rich "；The temperature for the cooling water that " the collecting distillate " uses
It is 25 DEG C.
2. the Chinese chestnut flower extract that claim 1 the method is prepared.
3. a kind of nutrient solution for skin care, including Chinese chestnut flower extract described in claim 2.
4. nutrient solution as claimed in claim 3, it is characterised in that: the nutrient solution further includes following ingredient: Sodium Hyaluronate,
1,3-PD, carbomer SF-1, D-pantothenyl aleohol, pungent glycol and Sensiva SC50.
5. nutrient solution as claimed in claim 4, it is characterised in that: in the nutrient solution, Chinese chestnut flower extract, hyaluronic acid
Sodium, 1,3-PD, carbomer SF-1, D-pantothenyl aleohol, the quality proportioning of pungent glycol and Sensiva SC50 are as follows: 88.9:2.0:
4.0:0.5:1.5:1.5:1.5.
6. nutrient solution as claimed in claim 3, it is characterised in that: the nutrient solution further includes following ingredient: Sodium Hyaluronate,
1,3-PD, carbomer SF-1, D-pantothenyl aleohol, pungent glycol, Sensiva SC50 and essence.
7. nutrient solution as claimed in claim 6, it is characterised in that: in the nutrient solution, Chinese chestnut flower extract, hyaluronic acid
Sodium, 1,3-PD, carbomer SF-1, D-pantothenyl aleohol, pungent glycol, the quality proportioning of Sensiva SC50 and essence are as follows: 88.9:
2.0:4.0:0.5:1.5:1.5:1.5:0.1.
8. a kind of facial mask is to load any nutrient solution in claim 3 to 7 on face mask substrate material to obtain.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102787019A (en) * | 2012-09-12 | 2012-11-21 | 迁西县板栗产业研究发展中心 | Extracting method of chestnut flower essential oil and chestnut flower hydrolat |
| KR20150047354A (en) * | 2013-10-24 | 2015-05-04 | 최성철 | A cosmetic composition containing chestnut, chestnut inner-shell and chestnut flower, and the method of preparing it |
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2016
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102787019A (en) * | 2012-09-12 | 2012-11-21 | 迁西县板栗产业研究发展中心 | Extracting method of chestnut flower essential oil and chestnut flower hydrolat |
| KR20150047354A (en) * | 2013-10-24 | 2015-05-04 | 최성철 | A cosmetic composition containing chestnut, chestnut inner-shell and chestnut flower, and the method of preparing it |
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