CN110243821A - Amur honeysuckle fruit pigment acid-base indicator and preparation method thereof - Google Patents
Amur honeysuckle fruit pigment acid-base indicator and preparation method thereof Download PDFInfo
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- CN110243821A CN110243821A CN201910426067.4A CN201910426067A CN110243821A CN 110243821 A CN110243821 A CN 110243821A CN 201910426067 A CN201910426067 A CN 201910426067A CN 110243821 A CN110243821 A CN 110243821A
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- amur honeysuckle
- acid
- base indicator
- honeysuckle fruit
- value
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
- G01N21/80—Indicating pH value
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- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Plasma & Fusion (AREA)
- Analytical Chemistry (AREA)
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- Engineering & Computer Science (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention provides a kind of Amur honeysuckle fruit pigment acid-base indicators and preparation method thereof.The Amur honeysuckle fruit pigment acid-base indicator shows different colours in different acidity condition;PH value is displayed in red for 1-4, and pH value is that 5-6 shows that yellow, pH value are 7-8 display green, and pH value is that 9-12 shows that yellow green, pH value are that 13-14 shows yellowish-brown.Make full use of Amur honeysuckle fruit, preparation method is environmentally protective, and preparation process is simple, raw material easily obtains, the color change of acid-base indicator is obvious, it is easy to differentiate, to solve the technical issues of acid-base indicator color change in the prior art is not abundant enough and color change differs smaller, is unfavorable for judgement and observes.
Description
Technical field
The invention belongs to acid-base indicator preparation technical fields, and in particular to a kind of Amur honeysuckle fruit pigment acid-base indicator
And preparation method thereof.
Background technique
Acid-base indicator is a kind of highly useful chemical reagent, for testing solution acid alkalinity, in acid base titration process
In, with the variation of solution concentration, structure changes, so as to cause the variation of color.Used soda acid currently on the market
Indicator, such as " wide pH value test paper ", usually organic synthesis product, to environment, there are qualitatively pollution and a side effects.
And generally only there are two types of colors for synthesis indicator, and color change is not abundant enough and color change difference is smaller, not sensitive enough, no
Conducive to judging and observe.
Amur honeysuckle also known as Flos Lonicerae maackii, cuckold bone, rhizoma cibotii bone, are Caprifoliaceae woodbines, and winter can bear circle
Kermesinus fruit, diameter are 5-6 millimeters, referred to as Amur honeysuckle fruit.Amur honeysuckle is a kind of machaka class plant, generally up to 6-
8 meters, rhizome diameter is up to 10 centimetres;On all sprouts, leaf two sides arteries and veins, petiole, bract, squamella and bract surface be all coated with short soft
Hair and micro- glandular hairs.Amur honeysuckle meeting fast-growth under strong sunlight irradiation, needs the time of direct sunlight generally not daily
Can be lower than 4 hours, it is slight drought-enduring, but still be grown in slightly moist environment it is best.Amur honeysuckle likes warm moderate ring
Border, and it is extremely cold-resistant, it outdoor can pass the winter in north of China overwhelming majority area.Amur honeysuckle has very healthy and strong trunk, energy
It is enough to survive under the conditions ofs different illumination, moisture, temperature, soil property etc..In the northeast in China, the north, east and the northwestward
Garden can plant, for ornamental.The fruit of Amur honeysuckle generally all raises up seed as seed, is not intended as fruit food
With.Very common wild fruit up to more than 50 in life, wherein such as wild jujube, hawthorn, persimmon, fructus hippophae, Chinese chestnut, hazel
Fruit, V. amurensis etc. is all edible, but such as fructus lycii including Amur honeysuckle fruit, Flos micheliae Albae fruit mouthfeel are bitter,
The mankind are inedible, and are easy to supervene the adverse reactions such as dizziness, nausea, diarrhea.Due to containing certain point in birds body
Enzyme is solved, birds are edible, can't generate adverse reaction.In addition to this, it Amur honeysuckle fruit and is underutilized.
Therefore, develop it is a kind of all there is different colors under different acid and alkaline conditions, color clear is persistently and easily
The environmentally protective acid-base indicator distinguished is highly important.
Summary of the invention
The main purpose of the present invention is to provide a kind of Amur honeysuckle fruit pigment acid-base indicator and preparation method thereof, the gold
Silver-colored wood fruit pigment acid-base indicator, makes full use of Amur honeysuckle fruit, and preparation method is environmentally protective, and preparation process is simple, former
Material easily obtains, and the color change of acid-base indicator is obvious, is easy to differentiate, and is become with solving acid-base indicator color in the prior art
It is smaller to change not abundant enough and color change difference, is unfavorable for the technical issues of judging and observing.
To achieve the goals above, according to the first aspect of the invention, a kind of Amur honeysuckle fruit pigment soda acid is provided to refer to
Show agent.
The Amur honeysuckle fruit pigment acid-base indicator shows different colours in different acidity condition;PH value shows red for 1-4
Color, pH value are that 5-6 shows that yellow, pH value are 7-8 display green, and pH value is that 9-12 shows that yellow green, pH value are that 13-14 display is yellow
Brown.
To achieve the goals above, according to the second aspect of the invention, a kind of Amur honeysuckle fruit pigment soda acid is provided to refer to
Show the preparation method of agent.
The preparation method of the Amur honeysuckle fruit pigment acid-base indicator the following steps are included:
S1: Amur honeysuckle fruit pigment extracting solution is prepared;
S2: it prepares acid-base indicator: the Amur honeysuckle fruit pigment extracting solution being subjected to centrifugal treating, supernatant is taken, obtains
To Amur honeysuckle fruit pigment acid-base indicator.
Further, the preparation method of the Amur honeysuckle fruit pigment extracting solution includes:
S1-1: Amur honeysuckle fruit being cleaned after draining, and is ground into the homogenate of Amur honeysuckle fruit;
S1-2: the Amur honeysuckle fruit homogenate in dipping solution is added, normal temperature and pressure under confined conditions, impregnate 2-3
Hour, Amur honeysuckle fruit pigment extracting solution is made.
Further, in S2 step, during the centrifugal treating, centrifugal rotational speed 6000r/min, centrifugation time is
15min。
Further, the Amur honeysuckle fruit homogenate and the weight ratio of the dipping solution are 1: 1-2.
Further, the dipping solution is ethanol water, concentration 75-95%.
Amur honeysuckle erythrocarpus is a kind of natural materials being extremely easy to get, and is had for the selection of extractant universal
Property, wherein dehydrated alcohol can serve as the optimum extraction agent of Amur honeysuckle erythrocarpus, it can be seen that, in the selection of extractant
On, Amur honeysuckle erythrocarpus compares other natural products also with greater advantage.In addition the soda acid that Amur honeysuckle fruit is prepared refers to
It is environmentally protective to show that agent has the advantages that, and the haematochrome that it is extracted all has not under different acid and alkaline conditions
Same color, color clear is lasting, is easy to distinguish.
In the present invention, acid-base indicator is prepared using natural products, wherein Amur honeysuckle fruit is mainly selected to carry out preparation acid
Neutralization indicator can reach effect identical with already present indicator on the market for the acid-base property for substance of testing.
The beneficial effects of the present invention are:
(1) preparation method is simple, and raw material easily obtains and preparation process green non-pollution;
(2) the Amur honeysuckle fruit pigment acid-base indicator prepared by is with a wide range of applications in analytical chemistry,
Color change is obvious, is easy to observe;
(3) pH value be in 1-4 Acidity Range it is red in stablizing, pH value is in 5-6 Acidity Range in stablizing yellow, pH value
For, in green is stablized, pH value is in yellow green is stablized in 9-12 Acidity Range, and pH value is 13-14 acidity model in 7-8 Acidity Range
It is in stablize yellowish-brown in enclosing;
(4) Amur honeysuckle fruit pigment acid-base indicator is pure natural product, and used organic reagent is recyclable, no environment
Pollution, has the advantages that chemical synthesis reagent does not have.
Specific embodiment
The illustrative embodiments of the disclosure are described in more detail below.Although showing the exemplary implementation of the disclosure
Mode, it being understood, however, that may be realized in various forms the disclosure without that should be limited by the embodiments set forth herein.Phase
Instead, it provides these embodiments to be to be able to thoroughly understand the disclosure, and can be complete by the scope of the present disclosure
It is communicated to those skilled in the art.
The invention discloses a kind of Amur honeysuckle fruit pigment acid-base indicator, which exists
Different acidity condition shows different colours;PH value is displayed in red for 1-4, and pH value is that 5-6 shows that yellow, pH value show green for 7-8
Color, pH value are that 9-12 shows that yellow green, pH value are that 13-14 shows yellowish-brown.
In the present embodiment, the raw material composition of Amur honeysuckle fruit pigment acid-base indicator includes that Amur honeysuckle fruit pigment extracts
Liquid is in different colours under different acid and alkaline conditions since Amur honeysuckle fruit pigment extracting solution color is by Effect of Acidity On Absorption, so
Amur honeysuckle fruit pigment acid-base indicator shows different colours in different acidity range, and is in 1-4 Acidity Range in pH value
It takes on a red color, pH value is in yellow in 5-6 Acidity Range, and pH value is in green in 7-8 Acidity Range, and pH value is 9-12 Acidity Range
Interior is in yellow green, and it is in yellowish-brown that pH value, which is in 13-14 Acidity Range, and above-mentioned colour developing is more stable, and color clear is lasting, is easy to distinguish
Not.In addition, it is prepared using Amur honeysuckle fruit, which has the advantages that environmentally protective.
The invention discloses the preparation methods of the Amur honeysuckle fruit pigment acid-base indicator, specifically includes the following steps:
S1: Amur honeysuckle fruit being cleaned after draining, and is ground into the homogenate of Amur honeysuckle fruit;
S2: it is 75-95% ethanol water that concentration is added in the homogenate of Amur honeysuckle fruit, and the two weight ratio is 1: 1-2,
Normal temperature and pressure under confined conditions, impregnates 2-3 hours, and Amur honeysuckle fruit pigment extracting solution is made.
S3: Amur honeysuckle fruit pigment extracting solution is subjected to centrifugal treating, centrifugal rotational speed 6000r/min, centrifugation time is
Then 15min takes supernatant, obtain Amur honeysuckle fruit pigment acid-base indicator.
Technical solution in order to better illustrate the present invention, the invention will be further described with reference to embodiments.
Embodiment 1:
The preparation method of Amur honeysuckle fruit pigment acid-base indicator in the present invention, comprising the following steps:
S1: fresh Amur honeysuckle fruit being cleaned after draining, and is ground into the homogenate of Amur honeysuckle fruit.
S2: 75% ethanol water, the homogenate of Amur honeysuckle fruit and 75% ethyl alcohol are added in the homogenate of above-mentioned Amur honeysuckle fruit
Aqueous solution weight ratio be 1: 1, then normal temperature and pressure under confined conditions, impregnate 2 hours, obtain Amur honeysuckle fruit pigment extraction
Liquid.
S3: above-mentioned Amur honeysuckle fruit extracting solution being centrifuged, revolving speed 6000r/min, is centrifuged 15min, is then taken
Clear liquid obtains Amur honeysuckle fruit acid-base indicator.
The color for the Amur honeysuckle fruit pigment acid-base indicator being prepared in embodiment 1 is in orange-yellow, using Accurate pH
Instrument detects its pH value, and the pH value that inspection side obtains is 5.35.
Embodiment 2:
The preparation method of Amur honeysuckle fruit pigment acid-base indicator in the present invention, comprising the following steps:
S1: fresh Amur honeysuckle fruit being cleaned after draining, and is ground into the homogenate of Amur honeysuckle fruit.
S2: 95% ethanol water, the homogenate of Amur honeysuckle fruit and 95% ethyl alcohol are added in the homogenate of above-mentioned Amur honeysuckle fruit
Aqueous solution weight ratio be 1: 1, then normal temperature and pressure under confined conditions, impregnate 2 hours, obtain Amur honeysuckle fruit pigment extracting solution.
S3: above-mentioned Amur honeysuckle fruit extracting solution being centrifuged, revolving speed 6000r/min, is centrifuged 15min, is then taken
Clear liquid obtains Amur honeysuckle fruit acid-base indicator.
The color for the Amur honeysuckle fruit pigment acid-base indicator being prepared in embodiment 2 is in orange-yellow, using Accurate pH
Instrument detects its pH value, and the pH value that inspection side obtains is 5.35.
Embodiment 3:
The preparation method of Amur honeysuckle fruit pigment acid-base indicator in the present invention, comprising the following steps:
S1: fresh Amur honeysuckle fruit being cleaned after draining, and is ground into the homogenate of Amur honeysuckle fruit.
S2: 80% ethanol water, the homogenate of Amur honeysuckle fruit and 80% ethyl alcohol are added in the homogenate of above-mentioned Amur honeysuckle fruit
Aqueous solution weight ratio be 1: 2, then normal temperature and pressure under confined conditions, impregnate 3 hours, obtain Amur honeysuckle fruit pigment extraction
Liquid.
S3: above-mentioned Amur honeysuckle fruit extracting solution being centrifuged, revolving speed 6000r/min, is centrifuged 15min, is then taken
Clear liquid obtains Amur honeysuckle fruit acid-base indicator.
The color for the Amur honeysuckle fruit pigment acid-base indicator being prepared in embodiment 3 is in orange-yellow, using Accurate pH
Instrument detects its pH value, and the pH value that inspection side obtains is 5.35.
Embodiment 4:
The preparation method of Amur honeysuckle fruit pigment acid-base indicator in the present invention, comprising the following steps:
S1: fresh Amur honeysuckle fruit being cleaned after draining, and is ground into the homogenate of Amur honeysuckle fruit.
S2: 85% ethanol water, the homogenate of Amur honeysuckle fruit and 85% ethyl alcohol are added in the homogenate of above-mentioned Amur honeysuckle fruit
Aqueous solution weight ratio be 1: 2, then normal temperature and pressure under confined conditions, impregnate 3 hours, obtain Amur honeysuckle fruit pigment extraction
Liquid.
S3: above-mentioned Amur honeysuckle fruit extracting solution being centrifuged, revolving speed 6000r/min, is centrifuged 15min, is then taken
Clear liquid obtains Amur honeysuckle fruit acid-base indicator.
The color for the Amur honeysuckle fruit pigment acid-base indicator being prepared in embodiment 4 is in orange-yellow, using Accurate pH
Instrument detects its pH value, and the pH value that inspection side obtains is 5.35.
The Amur honeysuckle fruit pigment acid-base indicator being prepared in embodiment 1-4 will be carried out by experiment below real
It tests.
One, experimental subjects
Experimental group is using the Amur honeysuckle fruit pigment acid-base indicator being prepared in embodiment 1-4;Control group is using existing
There are common methyl orange indicator and phenolphthalein indicator in technology.
Two, experimental method
Using common detection methods in the prior art to the Amur honeysuckle fruit pigment soda acid being prepared in embodiment 1-4
Phenolphthalein indicator in indicator and methyl orange indicator and comparative example 2 in comparative example 1, under different ph values into
The display experiment of row color.
Three, experimental result
The color of embodiment 1-4 is shown and is listed in the table below in 1 with pH value relationship.
The color of comparative example 1 and comparative example 2 is shown and is listed in the table below in 2 with pH value relationship.
The color characteristic table of Amur honeysuckle fruit pigment acid-base indicator under the different pH value of table 1
The color characteristic table of acid-base indicator in 2 comparative example 1 of table and comparative example 2
It analyzes known to Tables 1 and 2: being 5-6 Acidity Range in pH value in pH value to be red in stablizing in 1-4 Acidity Range
Interior pH value is in 7-8 Acidity Range in stablizing green in stablizing yellow, and pH value is in 9-12 Acidity Range in stablizing yellow green,
PH value is to develop the color more stable in 13-14 Acidity Range in yellowish-brown is stablized, and color clear is lasting, is easy to distinguish.It compares
In acid-base indicator in the prior art, Amur honeysuckle fruit pigment acid-base indicator is pure natural product, used organic examination
Agent is recyclable, and non-environmental-pollution has the advantages that chemical synthesis reagent does not have.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
In the technical scope disclosed by the present invention, any changes or substitutions that can be easily thought of by anyone skilled in the art,
It should be covered by the protection scope of the present invention.Therefore, protection scope of the present invention should be with the protection model of the claim
Subject to enclosing.
Claims (6)
1. a kind of Amur honeysuckle fruit pigment acid-base indicator, which is characterized in that the acid-base indicator is in different acidity condition
Show different colours;PH value is displayed in red for 1-4, and pH value is that 5-6 shows that yellow, pH value are 7-8 display green, pH value 9-12
Show that yellow green, pH value are that 13-14 shows yellowish-brown.
2. the preparation method of Amur honeysuckle fruit pigment acid-base indicator as described in claim 1, which is characterized in that including following
Step:
S1: Amur honeysuckle fruit pigment extracting solution is prepared;
S2: it prepares acid-base indicator: the Amur honeysuckle fruit pigment extracting solution being subjected to centrifugal treating, supernatant is taken, obtains gold
Silver-colored wood fruit pigment acid-base indicator.
3. the preparation method of Amur honeysuckle fruit pigment acid-base indicator according to claim 2, which is characterized in that the gold
The preparation method of silver-colored wood fruit pigment extracting solution includes:
S1-1: Amur honeysuckle fruit being cleaned after draining, and is ground into the homogenate of Amur honeysuckle fruit;
S1-2: the Amur honeysuckle fruit homogenate in dipping solution is added, normal temperature and pressure under confined conditions, impregnate 2-3 hours,
Amur honeysuckle fruit pigment extracting solution is made.
4. the preparation method of Amur honeysuckle fruit pigment acid-base indicator according to claim 2, which is characterized in that S2 step
In, during the centrifugal treating, centrifugal rotational speed 6000r/min, centrifugation time 15min.
5. the preparation method of Amur honeysuckle fruit pigment acid-base indicator according to claim 3, which is characterized in that the gold
Silver-colored wood fruit homogenate and the weight ratio of the dipping solution are 1: 1-2.
6. the preparation method of Amur honeysuckle fruit pigment acid-base indicator according to claim 3, which is characterized in that the leaching
Stain solution is ethanol water, concentration 75-95%.
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Cited By (1)
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CN112931492A (en) * | 2021-01-29 | 2021-06-11 | 天津农学院 | Crude extract of lonicera japonica fruit plant material ionized by strong acid H + ions and application of crude extract |
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2019
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CN101162219A (en) * | 2006-10-13 | 2008-04-16 | 广东海洋大学 | Pigment from mangrove kenaelia candel ph indicator |
CN108051541A (en) * | 2017-12-25 | 2018-05-18 | 战凯歌 | It is a kind of by the use of morning glory pigment as the method for acid base titration indicator |
Non-Patent Citations (1)
Title |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN112931492A (en) * | 2021-01-29 | 2021-06-11 | 天津农学院 | Crude extract of lonicera japonica fruit plant material ionized by strong acid H + ions and application of crude extract |
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