CN105594634A - 一种提高鲶鱼受精率的催产方法 - Google Patents
一种提高鲶鱼受精率的催产方法 Download PDFInfo
- Publication number
- CN105594634A CN105594634A CN201510950649.4A CN201510950649A CN105594634A CN 105594634 A CN105594634 A CN 105594634A CN 201510950649 A CN201510950649 A CN 201510950649A CN 105594634 A CN105594634 A CN 105594634A
- Authority
- CN
- China
- Prior art keywords
- parts
- catfish
- parent population
- spawning
- pond
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001233037 catfish Species 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 17
- 230000006698 induction Effects 0.000 title abstract 3
- 230000035558 fertility Effects 0.000 title abstract 2
- 241000251468 Actinopterygii Species 0.000 claims abstract description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 29
- 235000013601 eggs Nutrition 0.000 claims description 15
- 230000004720 fertilization Effects 0.000 claims description 14
- 230000037396 body weight Effects 0.000 claims description 12
- 239000000654 additive Substances 0.000 claims description 11
- 230000000996 additive effect Effects 0.000 claims description 11
- 241000143060 Americamysis bahia Species 0.000 claims description 9
- 150000004676 glycans Chemical class 0.000 claims description 9
- 229920001282 polysaccharide Polymers 0.000 claims description 9
- 239000005017 polysaccharide Substances 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 7
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 6
- 241000202807 Glycyrrhiza Species 0.000 claims description 6
- 240000004922 Vigna radiata Species 0.000 claims description 6
- 235000010721 Vigna radiata var radiata Nutrition 0.000 claims description 6
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 239000010231 banlangen Substances 0.000 claims description 6
- 230000002051 biphasic effect Effects 0.000 claims description 6
- 238000002347 injection Methods 0.000 claims description 6
- 239000007924 injection Substances 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 4
- 244000060020 Chamaerops excelsa Species 0.000 claims description 3
- 235000013164 Chamaerops excelsa Nutrition 0.000 claims description 3
- 241000252233 Cyprinus carpio Species 0.000 claims description 3
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 3
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims description 3
- 235000011613 Pinus brutia Nutrition 0.000 claims description 3
- 241000018646 Pinus brutia Species 0.000 claims description 3
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims description 3
- 229930003451 Vitamin B1 Natural products 0.000 claims description 3
- 229930003268 Vitamin C Natural products 0.000 claims description 3
- 150000001413 amino acids Chemical class 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 3
- 210000004556 brain Anatomy 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 claims description 3
- 239000011976 maleic acid Substances 0.000 claims description 3
- 230000032696 parturition Effects 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 229960003495 thiamine Drugs 0.000 claims description 3
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 3
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 3
- 235000010374 vitamin B1 Nutrition 0.000 claims description 3
- 239000011691 vitamin B1 Substances 0.000 claims description 3
- 235000019154 vitamin C Nutrition 0.000 claims description 3
- 239000011718 vitamin C Substances 0.000 claims description 3
- DBTMGCOVALSLOR-DEVYUCJPSA-N (2s,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-6-(hydroxymethyl)oxane-2,3,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](CO)O[C@H](O)[C@@H]2O)O)O[C@H](CO)[C@H]1O DBTMGCOVALSLOR-DEVYUCJPSA-N 0.000 claims description 2
- 229920001543 Laminarin Polymers 0.000 claims description 2
- 239000005717 Laminarin Substances 0.000 claims description 2
- 240000008866 Ziziphus nummularia Species 0.000 claims 2
- 230000002180 anti-stress Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 2
- 238000009395 breeding Methods 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 abstract 1
- 238000010276 construction Methods 0.000 abstract 1
- 230000012447 hatching Effects 0.000 abstract 1
- 230000009027 insemination Effects 0.000 abstract 1
- 230000017448 oviposition Effects 0.000 abstract 1
- 238000009344 polyculture Methods 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 6
- 241001247821 Ziziphus Species 0.000 description 4
- 241000251730 Chondrichthyes Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241001482311 Trionychidae Species 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/365—Lactones
- A61K31/375—Ascorbic acid, i.e. vitamin C; Salts thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
- A61K31/506—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim not condensed and containing further heterocyclic rings
- A61K31/51—Thiamines, e.g. vitamin B1
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
- A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/254—Acanthopanax or Eleutherococcus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
- A61K36/725—Ziziphus, e.g. jujube
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Zoology (AREA)
- Developmental Biology & Embryology (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Biomedical Technology (AREA)
- Endocrinology (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Reproductive Health (AREA)
- Gastroenterology & Hepatology (AREA)
- Animal Husbandry (AREA)
- Marine Sciences & Fisheries (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Neurology (AREA)
- Neurosurgery (AREA)
- Ophthalmology & Optometry (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
本发明公开了一种提高鲶鱼受精率的催产方法，包括如下步骤：（1）鱼池搭建、（2）亲鱼饲养、（3）亲鱼催产、（4）产卵和收集。本发明不同成分和操作的综合作用提高了鲶鱼的抗应激能力和生长活力，采用雌、雄亲鱼共同混养、自然受精的方式降低了人工劳动强度和对鱼的刺激，提高了受精卵的产量和质量，提升了受精卵的孵化率以及幼苗的成活率，有很好的推广价值。
Description
技术领域
本发明属于水产养殖领域，具体涉及一种提高鲶鱼受精率的催产方法。
背景技术
鲶鱼为底层凶猛性鱼类，喜欢生活在江河近岸的石隙、深坑、树根底部的土洞或石洞里，以及流速缓慢的水域。其营养丰富，每100克鱼肉中含水分64.1克、蛋白质14.4克，并含有多种矿物质和微量元素。除鲶鱼的鱼子有杂味不宜食用以外，全身是宝，鲶鱼是名贵的营养佳品，早在史书中就有记载，可以和鱼翅、野生甲鱼相媲美，它的食疗作用和药用价值是其他鱼类所不具备的，独特的强精壮骨和益寿作用是它独具的亮点。
因鲶鱼的营养价值较高，越来越多的人饲养鲶鱼，为了提高饲养效益，通常采用人工催情促进其产卵繁殖，目前多数采用的方案是对雄、雌鱼人工催情后，再捞出人工取精、取卵，促进其受精，此方式不仅费工费时，且会对鱼造成较大的应激反应，受精卵的产量和质量均会受影响。
发明内容
为了解决上述问题，本发明提供了一种提高鲶鱼受精率的催产方法，可提高鲶鱼的产卵数量和质量。
本发明通过以下技术方案来实现：
一种提高鲶鱼受精率的催产方法，包括如下步骤：
（1）鱼池搭建：搭建三个鱼池，分别为雄亲鱼池、雌亲鱼池和产卵鱼池，三个鱼池的四周侧壁和底部均设有LED绿光灯，雄亲鱼池和雌亲鱼池鱼的水深均为90~110cm、水温为18~20℃、溶氧量为5~10mg/L、PH值为6.5~7.5，产卵鱼池的水深为50~70cm、水温为23~26℃、溶氧量为8~12mg/L、PH值为6.5~7.5、底部设有棕榈皮制成的鱼巢；
（2）亲鱼饲养：选择体质健壮、无病无伤、成熟度好的亲鱼，雌亲鱼体重大于1.2kg，雄亲鱼体重大于0.8kg，分别放于雌亲鱼池和雄亲鱼池中饲养，同时向两鱼池水中按15~20g/L的量混入添加剂；
（3）亲鱼催产：向雌亲鱼背鳍基部注入其体重2.0~2.5%的催产剂，向雄亲鱼背鳍基部注入其体重1.0~1.2%的催产剂，隔8~10h后用相同剂量分别对其进行二次注射；
（4）产卵和收集：向产卵鱼池水中按20~30g/L的量混入添加剂，在二次注射4~6h后，将雌亲鱼和雄亲鱼按数量比1:1.1~1.3的量混合放入产卵鱼池中，待亲鱼产卵完毕后，迅速捕走亲鱼，随后对受精卵进行收集、孵化操作。
进一步的，步骤（1）所述LED绿光灯的光照强度为200~300lx。
进一步的，步骤（2）、（4）所述的添加剂由如下重量份的物质制成：5~10份钩藤、4~6份枣仁、3~5份板蓝根、2~4份刺五加、4~8份甘草、2~3份绿豆、1~3份丰年虾、0.2~0.5份活性多糖、0.1~0.3份氨基酸；
其制备方法包括如下步骤：
（1）将5~10份钩藤、4~6份枣仁、3~5份板蓝根、2~4份刺五加、4~8份甘草、2~3份绿豆放入其质量3~5倍的清水中，武火煮沸后再文火煎煮15~20min，滤出滤液A备用；
（2）将1~3份丰年虾与等质量的清水混合后高速搅拌，将丰年虾绞碎成糜状后静置2~3h，再过滤得滤液B备用；
（3）将上述物质同剩余成分充分混拌均匀后即可。
进一步的，所述活性多糖由灵芝多糖、枸杞多糖、海带多糖和松花粉多糖的至少一种组成。
进一步的，步骤（3）所述的催产剂由如下重量份的物质组成：2~3份绒毛膜促性腺激素（HCG）、1.5~2.5份马来酸地欧酮（DOM）、5~8份鲤鱼脑下垂体(PG)、1~2份复合维生素、5~10份水。
进一步的，所述复合维生素含有维生素B1、维生素B9和维生素C。
本发明具有如下有益效果：
（1）本发明在池水中施加的添加剂可提高鲶鱼的抗应激能力和机体体质，保证了鲶鱼正常产卵受精。
（2）利用光源刺激鱼类生长和增产的报道屡见不鲜，而利用光源促进鲶鱼产卵却鲜少见到，本发明通过实践发现低强度绿光可提高鲶鱼受精卵的产量和质量，且可增强鲶鱼的生长活力。
（3）本发明催产剂中添加的复合维生素亦可增强鲶鱼的抗应激能力，并可增强其他成分的作用效果。
（4）本发明不同成分和操作的综合作用提高了鲶鱼的抗应激能力和生长活力，采用雌、雄亲鱼共同混养、自然受精的方式降低了人工劳动强度和对鱼的刺激，提高了受精卵的产量和质量，提升了受精卵的孵化率以及幼苗的成活率，有很好的推广价值。
具体实施方式
实施例1
一种提高鲶鱼受精率的催产方法，包括如下步骤：
（1）鱼池搭建：搭建三个鱼池，分别为雄亲鱼池、雌亲鱼池和产卵鱼池，三个鱼池的四周侧壁和底部均设有LED绿光灯，雄亲鱼池和雌亲鱼池鱼的水深均为90~100cm、水温为18~20℃、溶氧量为5~10mg/L、PH值为6.5~7.5，产卵鱼池的水深为60~70cm、水温为23~26℃、溶氧量为8~12mg/L、PH值为6.5~7.5、底部设有棕榈皮制成的鱼巢；
（2）亲鱼饲养：选择体质健壮、无病无伤、成熟度好的亲鱼，雌亲鱼体重大于1.2kg，雄亲鱼体重大于0.8kg，分别放于雌亲鱼池和雄亲鱼池中饲养，同时向两鱼池水中按16g/L的量混入添加剂；
（3）亲鱼催产：向雌亲鱼背鳍基部注入其体重2.4%的催产剂，向雄亲鱼背鳍基部注入其体重1.2%的催产剂，隔8h后用相同剂量分别对其进行二次注射；
（4）产卵和收集：向产卵鱼池水中按25g/L的量混入添加剂，在二次注射6h后，将雌亲鱼和雄亲鱼按数量比1:1.2的量混合放入产卵鱼池中，待亲鱼产卵完毕后，迅速捕走亲鱼，随后对受精卵进行收集、孵化操作。
进一步的，步骤（1）所述LED绿光灯的光照强度为200~300lx。
进一步的，步骤（2）、（4）所述的添加剂由如下重量份的物质制成：8份钩藤、4份枣仁、3份板蓝根、2份刺五加、5份甘草、3份绿豆、2份丰年虾、0.3份活性多糖、0.2份氨基酸；
其制备方法包括如下步骤：
（1）将8份钩藤、4份枣仁、3份板蓝根、2份刺五加、5份甘草、3份绿豆放入其质量3倍的清水中，武火煮沸后再文火煎煮15~20min，滤出滤液A备用；
（2）将2份丰年虾与等质量的清水混合后高速搅拌，将丰年虾绞碎成糜状后静置2h，再过滤得滤液B备用；
（3）将上述物质同剩余成分充分混拌均匀后即可。
进一步的，所述活性多糖由灵芝多糖和松花粉多糖组成。
进一步的，步骤（3）所述的催产剂由如下重量份的物质组成：3份绒毛膜促性腺激素（HCG）、2份马来酸地欧酮（DOM）、6份鲤鱼脑下垂体(PG)、2份复合维生素、8份水。
进一步的，所述复合维生素含有维生素B1、维生素B9和维生素C。
对比例1
本对比例在水池内不设LED绿光灯，其余方法步骤与实施例1完全相同。
对比例2
本对比例在池水中不施加添加剂，其余方法步骤与实施例1完全相同。
以土鲶作为养殖对象，现有普通催产方法作为空白对照组，每组雌亲鱼80尾、雄亲鱼96尾，下表为相应的数据对比：
| 实施例1 | 对比例1 | 对比例2 | 空白对照组 | |
| 受精卵粒数（万个） | 61.2 | 55.3 | 54.7 | 50.8 |
| 受精卵孵化率（%） | 96.8 | 94.1 | 93.2 | 88.7 |
| 幼苗成活率（%） | 92.6 | 85.8 | 84.6 | 80.5 |
Claims (6)
1.一种提高鲶鱼受精率的催产方法，其特征在于，包括如下步骤：
（1）鱼池搭建：搭建三个鱼池，分别为雄亲鱼池、雌亲鱼池和产卵鱼池，三个鱼池的四周侧壁和底部均设有LED绿光灯，雄亲鱼池和雌亲鱼池鱼的水深均为90~110cm、水温为18~20℃、溶氧量为5~10mg/L、PH值为6.5~7.5，产卵鱼池的水深为50~70cm、水温为23~26℃、溶氧量为8~12mg/L、PH值为6.5~7.5、底部设有棕榈皮制成的鱼巢；
（2）亲鱼饲养：选择体质健壮、无病无伤、成熟度好的亲鱼，雌亲鱼体重大于1.2kg，雄亲鱼体重大于0.8kg，分别放于雌亲鱼池和雄亲鱼池中饲养，同时向两鱼池水中按15~20g/L的量混入添加剂；
（3）亲鱼催产：向雌亲鱼背鳍基部注入其体重2.0~2.5%的催产剂，向雄亲鱼背鳍基部注入其体重1.0~1.2%的催产剂，隔8~10h后用相同剂量分别对其进行二次注射；
（4）产卵和收集：向产卵鱼池水中按20~30g/L的量混入添加剂，在二次注射4~6h后，将雌亲鱼和雄亲鱼按数量比1:1.1~1.3的量混合放入产卵鱼池中，待亲鱼产卵完毕后，迅速捕走亲鱼，随后对受精卵进行收集、孵化操作。
2.根据权利要求1所述的一种提高鲶鱼受精率的催产方法，其特征在于，步骤（1）所述LED绿光灯的光照强度为200~300lx。
3.根据权利要求1所述的一种提高鲶鱼受精率的催产方法，其特征在于，步骤（2）、（4）所述的添加剂由如下重量份的物质制成：5~10份钩藤、4~6份枣仁、3~5份板蓝根、2~4份刺五加、4~8份甘草、2~3份绿豆、1~3份丰年虾、0.2~0.5份活性多糖、0.1~0.3份氨基酸；
其制备方法包括如下步骤：
（1）将5~10份钩藤、4~6份枣仁、3~5份板蓝根、2~4份刺五加、4~8份甘草、2~3份绿豆放入其质量3~5倍的清水中，武火煮沸后再文火煎煮15~20min，滤出滤液A备用；
（2）将1~3份丰年虾与等质量的清水混合后高速搅拌，将丰年虾绞碎成糜状后静置2~3h，再过滤得滤液B备用；
（3）将上述物质同剩余成分充分混拌均匀后即可。
4.根据权利要求3所述的一种提高鲶鱼受精率的催产方法，其特征在于，所述活性多糖由灵芝多糖、枸杞多糖、海带多糖和松花粉多糖的至少一种组成。
5.根据权利要求1所述的一种提高鲶鱼受精率的催产方法，其特征在于，步骤（3）所述的催产剂由如下重量份的物质组成：2~3份绒毛膜促性腺激素（HCG）、1.5~2.5份马来酸地欧酮（DOM）、5~8份鲤鱼脑下垂体(PG)、1~2份复合维生素、5~10份水。
6.根据权利要求5所述的一种提高鲶鱼受精率的催产方法，其特征在于，所述复合维生素含有维生素B1、维生素B9和维生素C。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510950649.4A CN105594634A (zh) | 2015-12-19 | 2015-12-19 | 一种提高鲶鱼受精率的催产方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510950649.4A CN105594634A (zh) | 2015-12-19 | 2015-12-19 | 一种提高鲶鱼受精率的催产方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105594634A true CN105594634A (zh) | 2016-05-25 |
Family
ID=55975455
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510950649.4A Pending CN105594634A (zh) | 2015-12-19 | 2015-12-19 | 一种提高鲶鱼受精率的催产方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105594634A (zh) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004080165A1 (fr) * | 2003-03-10 | 2004-09-23 | Institut Francais De Recherche Pour L'exploitation De La Mer (Ifremer) | Procede d'aquaculture et produits obtenus selon ce procede |
| CN101779748A (zh) * | 2010-03-16 | 2010-07-21 | 苏兴 | 一种水产养殖虾蟹鱼用活力增强剂及其制备方法 |
| CN102488753A (zh) * | 2011-12-23 | 2012-06-13 | 天津康莱森生物科技集团有限公司 | 一种具有抗菌消炎作用的渔用中药组合物及其制备方法 |
| CN103960170A (zh) * | 2013-01-29 | 2014-08-06 | 易湘开 | 湘鲶的人工繁育和养殖方法 |
| US8919744B1 (en) * | 2011-08-10 | 2014-12-30 | The United States Of America As Represented By The Secretary Of Agriculture | Water aeration system and method |
| CN104705225A (zh) * | 2014-12-15 | 2015-06-17 | 天津市宽余水产养殖专业合作社 | 一种大口鲶人工繁殖方法 |
-
2015
- 2015-12-19 CN CN201510950649.4A patent/CN105594634A/zh active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004080165A1 (fr) * | 2003-03-10 | 2004-09-23 | Institut Francais De Recherche Pour L'exploitation De La Mer (Ifremer) | Procede d'aquaculture et produits obtenus selon ce procede |
| CN101779748A (zh) * | 2010-03-16 | 2010-07-21 | 苏兴 | 一种水产养殖虾蟹鱼用活力增强剂及其制备方法 |
| US8919744B1 (en) * | 2011-08-10 | 2014-12-30 | The United States Of America As Represented By The Secretary Of Agriculture | Water aeration system and method |
| CN102488753A (zh) * | 2011-12-23 | 2012-06-13 | 天津康莱森生物科技集团有限公司 | 一种具有抗菌消炎作用的渔用中药组合物及其制备方法 |
| CN103960170A (zh) * | 2013-01-29 | 2014-08-06 | 易湘开 | 湘鲶的人工繁育和养殖方法 |
| CN104705225A (zh) * | 2014-12-15 | 2015-06-17 | 天津市宽余水产养殖专业合作社 | 一种大口鲶人工繁殖方法 |
Non-Patent Citations (1)
| Title |
|---|
| 李传武: "南方大口鲶生物学及其养殖技术概述", 《现代渔业信息》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Dunham et al. | Production of hybrid catfish | |
| CN101940184B (zh) | 一种改良鲫的育种与养殖方法 | |
| CN105594635A (zh) | 一种优质高产鲶鱼的养殖方法 | |
| CN109511579B (zh) | 尖裸鲤的人工繁殖技术 | |
| CN101755699B (zh) | 细鳞鲑苗种培育方法 | |
| CN109819913A (zh) | 一种密斑刺鲀的人工繁育方法 | |
| CN100388880C (zh) | 获得大菱鲆优质受精卵的方法 | |
| CN101647412B (zh) | 纹缟虾虎鱼人工催产授精繁育方法 | |
| CN103651220A (zh) | 抚仙四须鲃人工繁殖技术 | |
| CN107711621A (zh) | 一种金钱鱼的繁育方法 | |
| CN101658146A (zh) | 罗非鱼耐盐育种技术 | |
| CN102369907A (zh) | 采用人工受精选育特种野鸡的方法 | |
| CN106879502A (zh) | 雌黄颡鱼与雄瓦氏黄颡鱼的杂交方法、复合饲料添加剂 | |
| CN101011038A (zh) | 一种鲮鱼作为鳜鱼活饵料鱼的高产养殖方法 | |
| CN102792910B (zh) | 一种金鳟全雌杂交苗种的培育方法 | |
| CN107439424A (zh) | 一种津新乌鲫的制种和养殖方法 | |
| CN102812913A (zh) | 一种杂交全雌金鳟鱼苗种的培育方法 | |
| CN105746398A (zh) | 一种多鳞铲颌鱼的鱼苗孵化方法 | |
| CN115735810A (zh) | 培育生长速度快的全雄马口鱼的方法 | |
| CN108165522A (zh) | 一种提高肉雏鸡质量和促进早期生长的胚期补剂及其应用 | |
| CN107926770A (zh) | 赤点石斑鱼与鞍带石斑鱼远缘杂交育种方法 | |
| CN105210950B (zh) | 一种鲟鱼的人工培育方法 | |
| CN105594634A (zh) | 一种提高鲶鱼受精率的催产方法 | |
| CN106172095B (zh) | 一种大规格长江水系河蟹良种的选育方法 | |
| Jayasankar et al. | Freshwater aquaculture in India |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160525 |
|
| RJ01 | Rejection of invention patent application after publication |