CN105582530A - Compound biological protease - Google Patents

Abstract

The invention discloses a compound biological protease which is prepared by adding syrup into grain raw materials, adding syrup into traditional Chinese medicine raw materials, and carrying out natural fermentation. The compound biological protease is characterized in that the grain raw materials respectively consist of rice, millet, wheat, buckwheat, sorghum, soybean, mung bean, black sesame and corn; and the traditional Chinese medicine raw materials respectively consist of fleece flower root, Mongolian dandelion herb, notoginseng, tangerine peel, tuckahoe, Chinese yam, eucommia bark, barbary wolfberry fruit, Chinese angelica, lily and tea leaf. The compound biological protease can be directly processed into an oral liquid dosage form for preventing and treating gastropathy. The compound biological protease has the effects of removing toxicity for eliminating carbuncles, loosening the bowels to relieve constipation, and strengthening the spleen and stomach, can enhance the immunologic function of organisms, and has obvious effects of preventing and treating gastropathy and duodenal ulcer.

Description

A kind of composite biological protein enzyme

Technical field

The present invention relates to composite biological protein enzyme oral liquid field. This composite biological protein enzyme can directly be processed into prevention and the treatment of oral liquid formulation for stomach trouble.

Background technology

Protease is most important a kind of industrial enzyme preparation, can catalytic proteins and polypeptide peptide bond hydrolysis. It is extensively present in pluck, plant stem-leaf, fruit and microorganism. Various organisms can be synthesized it, have productive value but have microbial protease only. Up to the present, on international market, commercially available protein enzyme class is more. China Patent No. 2004100212976 discloses a kind of nanometer hepatocuprein and preparation method thereof, diameter 10-200nm, antioxidation activity improves more than 10 times, skin, mucosa absorption rate are more than 90%, two of Increased Plasma Half-lifes are more than the order of magnitude, can enter the effect that in cell, directly free radical is removed in performance, there is stronger lipoid peroxidization resistant; With the purifying SOD extracting in plant or animal origin be raw material, first adopt the homogeneous technology of nm super high, be processed into nanometer SOD molecule, apply again microemulsified or liposome technology and make the dispersion of nanometer SOD molecule, emulsification, form nanometer SOD droplet, or nanometer SOD liquid is made nanometer SOD micro-capsule by application supersonic jet technology. Nanometer SOD droplet/micro-capsule can directly be processed into soft capsule, capsule or other formulation for prevention and treatment of diseases; Also can be used as intermediate for fields such as food, health products, medicine, cosmetics, daily-use chemical industries.

Summary of the invention

The object of the present invention is to provide a kind of composite biological protein enzyme, can directly be processed into prevention and the treatment of oral liquid formulation for stomach trouble. There is removing toxicity for eliminating carbuncles, relax bowel, strengthening the spleen and stomach effect, can enhanced machine body immunity function, stomach trouble, duodenal ulcer are had to obvious preventive and therapeutic action.

For achieving the above object, the present invention has adopted such technical scheme: described composite biological protein enzyme, is characterized in that: be made up through spontaneous fermentation of cereal materials sweet water adding and traditional Chinese medicinal material raw materials sweet water adding; Cereal materials is made up of rice, millet, wheat, buckwheat, Chinese sorghum, soya bean, mung bean, Semen sesami nigrum, corn respectively; Traditional Chinese medicinal material raw materials is made up of the fleece-flower root, dandelion, pseudo-ginseng, dried orange peel, Poria cocos, Chinese yam, the bark of eucommia, matrimony vine, Radix Angelicae Sinensis, lily, tealeaves respectively.

The preparation method of described composite biological protein enzyme is following steps: step 1, according to rice, millet, wheat, buckwheat, Chinese sorghum, soya bean, mung bean, Semen sesami nigrum, the corn of weight portion selection high-quality, and it is fully mixed, obtain cereal materials for subsequent use; Step 2, according to weight portion edible sugar and edible brown sugar or wherein a kind of, with drinking water, fully stir, obtain syrup, for subsequent use; Step 3, the cereal materials that step 1 and step 2 are obtained fully mix with syrup, are placed in airtight container spontaneous fermentation under normal temperature environment; After filtration, obtain cereal materials fermented liquid, for subsequent use;

Step 4, according to the fleece-flower root, dandelion, pseudo-ginseng, dried orange peel, Poria cocos, Chinese yam, the bark of eucommia, matrimony vine, Radix Angelicae Sinensis, lily, the tealeaves of weight portion selection high-quality, and it is fully mixed, obtain traditional Chinese medicinal material raw materials, for subsequent use; Step 5, according to weight portion edible sugar and edible brown sugar or wherein a kind of, with drinking water, fully stir, obtain syrup, for subsequent use; Step 6, the traditional Chinese medicinal material raw materials that step 4 and step 5 are obtained fully mix with syrup, are placed in airtight container spontaneous fermentation under normal temperature environment; After filtration, obtain traditional Chinese medicinal material raw materials fermented liquid, for subsequent use;

Step 7, the cereal materials fermented liquid that step 3 and step 6 are obtained fully mix with traditional Chinese medicinal material raw materials fermented liquid, are placed in airtight container spontaneous fermentation under normal temperature environment; After filtration, obtain composite biological protein enzyme; Obtain composite biological protein enzyme carry out canned, obtain oral liquid formulations.

In traditional Chinese medicinal material raw materials, its effect respectively:

The fleece-flower root: effect removing toxic substances of Radix Polygoni Multiflori, the carbuncle that disappears, relaxes bowel. For scrofula sore carbuncle, rubella itch, the dry constipation of intestines, hyperlipidemia. RADIX POLYGONI MULTIFLORI PREPARATA: filling liver kidney, benefiting essence-blood, black beard and hair, strengthening the bones and muscles; For under dizzy tinnitus, poliosis, soreness and weakness of waist and knees, extremity numbness, uterine bleeding band, hyperlipidemia. Pharmacological evaluation proves, prepared fleece flower root can promote red blood cell to generate, and promotes hematopoiesis function, strengthen immunologic function, reduce blood sugar, cardiac muscle is had to excitation, energy reducing heart rate, increase coronary blood flow, can reduce cholesterol, alleviate atherosclerotic, protect the liver, delay senility, and have the effect that promotes that intestinal tube is wriggled.

Dandelion: belong to composite family herbaceos perennial. In dandelion plant, contain the multiple health-nutrition compositions such as taraxol, taraxacin, choline, organic acid, synanthrin; Contain protein, fat, carbohydrate, trace element and vitamin etc. simultaneously, have abundant nutritive value. [nature,taste and action]: sweet, micro-hardship, cold. Clearing heat and detoxicating, dispersing swelling and dissipating binds. [curing mainly]: the infection of the upper respiratory tract, eye conjunctivitis, mumps, hyperglycaemia, acute mastitis swells and ache, gastritis, dysentery, hepatitis, courage is assisted inflammation, acute appendicitis, urinary infection, pelvic infecton, carbuncle furuncle furunculosis, pharyngitis, controls acute mastitis, adenolymphitis, scrofula, malignant boil carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, urinary tract infections.

Pseudo-ginseng: Compendium of Material Medica is described, pseudo-ginseng " taste is micro-sweet and bitter, quite like the taste of ginseng ", has the title of " invaluable ". Raw with there being hemostasis cardiac stimulant, the loose stasis of blood is raw new, the remarkable function of detumescence ding-tong, ripe use have invigorate blood circulation, enrich blood, effect of strong qi-restoratives. Its root, stem, leaf, Hua Junke are used as medicine. Conventionally the pseudo-ginseng of saying, refers to the dry root of pseudo-ginseng plant. Saponin is the main component of ginseng, to strengthening muscle power, improves Myocardial Oxygen Metabolism, and the tolerance that improves Animal Anoxia has effect very much. Topmost medicinal ingredient tetracyclic triterpene saponin in ginseng, pseudo-ginseng not only contains equally, and higher than ginseng, the contained flavone compound of pseudo-ginseng, has adjusting blood pressure, coronary artery dilator, increase CF, reduce heart oxygen demand, the effect that alleviates myocardium work load. Clinical Practice proves, uncomfortable in chest, angina cordis that pseudo-ginseng is caused by coronary heart disease in treatment have satisfied curative effect, also very remarkable to reducing the function of cholesterol and blood fat. Therefore pseudo-ginseng is except falling as treatment traditional famous medicine of beating blood stasis, also increasingly extensively as prevention and cure of cardiovascular disease and the nutrient health improving the health.

Dried orange peel: the appetizing of regulating the flow of vital energy, eliminating dampness and eliminating phlegm, control the spleen-stomach diseases. Containing volatile oil 1.5%(milling process) to the 2%(way of distillation), in oil, main component is D-citrene, also, containing beta-myrcene and nopinene (pinene) etc., separately remove first dihydro Nobiletin containing flavones ingredient hesperidine, neohesperidin, naringenin, dihydro Nobiletin and 5-; Report that again river dried orange peel is containing hesperidine approximately 8.4%, the about 0.15%. of Nobiletin is separately containing synephrine (oxedrine) 0.28%-2.54%. [attending effectiveness]: regulating qi-flowing for strengthening spleen, in tune, eliminating dampness, reduces phlegm. Cure mainly the abdominal fullness and distention of the taste stagnation of the circulation of vital energy or pain, indigestion. Abdominal distension uncomfortable in chest, indigestion and loss of appetite loose stool in wet turbid resistance. The wet cough and asthma of stopping up lung of phlegm. For chest gastral cavity turgor, food is few vomits and diarrhoea, coughing with a lot of sputum.

Poria cocos: the dry sclerotia of On Polyporaceae Poria cocos. Because its effect is very extensive, regardless of the four seasons, by it and various compatibility of drugs, no matter cold, temperature, wind, all diseases that wets can be brought into play its unique effects. Poria cocos taste is sweet, light, property is flat, be used as medicine there is clearing damp and promoting diuresis, the function of beneficial spleen and stomach, antitoxic heart-soothing and sedative. Modern medicine study: Poria cocos energy enhanced machine body immunity function, pachymaran has significantly antitumor and protect the liver dirty effect. Poria cocos slightly sweet flavor is flat, enters the heart, lung, the spleen channel. There is eliminating dampness and diuresis, strengthening the spleen and stomach, effect of antitoxic heart-soothing and sedative. Can control difficult urination, oedema turgor, phlegm and retained fluid is coughed contrary, vomits contrary, and vomiting during early pregnancy, has loose bowels, seminal emission, stranguria with turbid discharge, palpitation with fear, forgetful disease such as grade.

Chinese yam: [source] these product are the dry rhizome of Dioscoreaceae plant Chinese yam. [nature and flavor with return through] taste is sweet, and property is flat. Return spleen, lung, kidney channel. [function with cure mainly] tonifying spleen nourishing the stomach, the beneficial lung that promotes the production of body fluid, the puckery essence of kidney tonifying. Modern pharmacological research confirms, RHIIZOMA DIOSCOREAE from Henan of China has nutritious tonifying, induce disturb element, strengthen immunity of organisms, excretion in scheduling, tonifying Qi are promoted blood circulation, kobadrin, relieving asthma etc. and to help to change, coronary artery and micro-samsara blood flow can be improved, chronic bronchitis, hat unpleasantness, angina pectoris etc. can be treated.

The bark of eucommia: the medicinal bark of eucommia, be the dry bark of the Eucommiaceae plant bark of eucommia, be Chinese famous and precious tonic herb. Its taste is sweet, warm in nature. Have tonify the liver and kidney, strengthen muscles and bones, coordinating Chong and Conception Vessels, solid through antiabortive effect. Can treat pain in waist and lower extremities that kidney-yang deficiency causes or aching and limp unable, born of the same parents' tire that irritability void causes is solid, scrotum the is wet disease such as itch. In Shennong's Herbal, be listed in top grade.

Matrimony vine: be the dry mature fruit of plant of Solanaceae lycium barbarum. Its taste is sweet, the flat effect with liver-kidney tonifying of property, says and " take for a long time hard muscles and bones, make light of one's life by commiting suicide not old, cold-resistant heat in Compendium of Material Medica. " traditional Chinese medical science conventional it treat liver-kidney Yin deficiency, soreness and weakness of waist and knees, dizziness, forgetful, dizzy, the many tear of blurred vision, quench one's thirst, the illness such as seminal emission. Modern pharmacology studies confirm that the adjustable body's immunity of the fruit of Chinese wolfberry, can effectively suppress tumor growth and cell mutation, have delay senility, the effect of AFL, adjusting blood fat and the aspect such as blood sugar, promotion hematopoiesis function.

Radix Angelicae Sinensis: [chemical composition] contains basic lactone, n-butene acyl lactone, forulic acid, nicotinic acid, sucrose and several amino acids, and sesquiterpenoids etc. [meridian distribution of property and flavor] is warm in nature, hides sweet, pungent. Return liver, the heart, the spleen channel. [function cures mainly] enriched blood and invigorated blood circulation, and menstruction regulating and pain relieving relaxes bowel. For sallow, the dizzy palpitaition of the deficiency of blood, irregular menstruation, through closing dysmenorrhoea, asthenia cold abdominalgia, the dry constipation of intestines, arthralgia due to wind-dampness, injury from falling down, ulcer sores.

Lily: the traditional Chinese medical science thinks that lily has Yin nourishing and lung moistening, clearing away the heart fire and tranquillizing effect. The main deficiency of Yin is coughed for a long time; Blood-stained sputum; The pyreticosis later stage; Waste heat is unclear, or dysphoria palpitation with fear, the insomnia and dreamful sleep, absent-minded of feelings will due to unsuccessful; Carbuncle is swollen; Wet sore.

Tealeaves: the Main Ingredients and Appearance that tealeaves has pharmacological action is Tea Polyphenols, caffeine, lipopolysaccharides, theanine etc. Contribute to suppress and resist viral bacterium Tea Polyphenols have stronger astriction, pathogen, virus are had to obvious inhibition and killing action, anti-inflammatory antidiarrheal is had to positive effect.

In the present invention, the abundant nutrition material and the protein that in cereal materials, contain, by above-mentioned steps, the cereal materials fermented liquid that its fermentation is become contain abundant nutrition material and microbial protease; By fully mixing, ferment with traditional Chinese medicinal material raw materials fermented liquid, acquisition has the composite biological protein enzyme of therapeutic efficiency to stomach trouble and duodenal ulcer; And ensure efficacy of drugs and cereal materials fermented liquid in nutriment fully warm, not only make drug effect gentle, the removing toxicity for eliminating carbuncles of strengthening traditional Chinese medicinal material raw materials, relax bowel, strengthening the spleen and stomach effect, and be easily absorbed by the body, obviously reduce and eliminate the toxic and side effect of Chinese medicine.

The present invention confirms that through concrete application example chronic, superficial gastritis, atrophic gastritis, the routed soup of gastroduodenal are all had to obvious result for the treatment of; The outpatient service of First People's Hospital of Dengfeng City accepts for medical treatment and utilizes painless gastroscope to carry out digestive system detection to patient, is diagnosed as according to check result: chronic, superficial gastritis, atrophic gastritis, the gastroduodenal soup of bursting. Utilize oral liquid of the present invention to treat, its using method: oral each 10-20 milliliter, every day 3-4 time.

1. 36 of chronic, superficial gastritis patients,, every day 4 times, serve on 7 by oral each 15 milliliters. Cure 31 examples, all the other significant effective;

2. 32 of patients with atrophic gastritis,, every day 6 times, serve on 9 by oral each 15 milliliters. Cure 30 examples, all the other significant effective;

3. 29 of the routed soup of gastroduodenal,, every day 6 times, serve on 14 by oral each 20 milliliters. Cure 23 examples, all the other significant effective.

Detailed description of the invention

Composite biological protein enzyme of the present invention, is made up through spontaneous fermentation of cereal materials sweet water adding and traditional Chinese medicinal material raw materials sweet water adding. It is characterized in that, cereal materials is made up of rice, millet, wheat, buckwheat, Chinese sorghum, soya bean, mung bean, Semen sesami nigrum, corn respectively; Traditional Chinese medicinal material raw materials is made up of the fleece-flower root, dandelion, pseudo-ginseng, dried orange peel, Poria cocos, Chinese yam, the bark of eucommia, matrimony vine, Radix Angelicae Sinensis, lily, tealeaves respectively.

The preparation method of described composite biological protein enzyme is following steps:

Step 1, according to rice, millet, wheat, buckwheat, Chinese sorghum, soya bean, mung bean, Semen sesami nigrum, the corn of weight portion selection high-quality, and it is fully mixed, obtain cereal materials for subsequent use;

Step 2, according to weight portion edible sugar and edible brown sugar or wherein a kind of, with drinking water, fully stir, obtain syrup, for subsequent use;

Step 3, the cereal materials that step 1 and step 2 are obtained fully mix with syrup, are placed in airtight container spontaneous fermentation under normal temperature environment; After filtration, obtain cereal materials fermented liquid, for subsequent use;

Step 4, according to the fleece-flower root, dandelion, pseudo-ginseng, dried orange peel, Poria cocos, Chinese yam, the bark of eucommia, matrimony vine, Radix Angelicae Sinensis, lily, the tealeaves of weight portion selection high-quality, and it is fully mixed, obtain traditional Chinese medicinal material raw materials, for subsequent use;

Step 5, according to weight portion edible sugar and edible brown sugar or wherein a kind of, with drinking water, fully stir, obtain syrup, for subsequent use;

Step 6, the traditional Chinese medicinal material raw materials that step 4 and step 5 are obtained fully mix with syrup, are placed in airtight container spontaneous fermentation under normal temperature environment; After filtration, obtain traditional Chinese medicinal material raw materials fermented liquid, for subsequent use;

Step 7, the cereal materials fermented liquid that step 3 and step 6 are obtained fully mix with traditional Chinese medicinal material raw materials fermented liquid, are placed in airtight container spontaneous fermentation under normal temperature environment; After filtration, obtain composite biological protein enzyme; Obtain composite biological protein enzyme carry out canned, obtain oral liquid formulations.

Claims (2)

1. a composite biological protein enzyme, is made up through spontaneous fermentation of cereal materials sweet water adding and traditional Chinese medicinal material raw materials sweet water adding; It is characterized in that, cereal materials is made up of rice, millet, wheat, buckwheat, Chinese sorghum, soya bean, mung bean, Semen sesami nigrum, corn respectively; Traditional Chinese medicinal material raw materials is made up of the fleece-flower root, dandelion, pseudo-ginseng, dried orange peel, Poria cocos, Chinese yam, the bark of eucommia, matrimony vine, Radix Angelicae Sinensis, lily, tealeaves respectively.

2. composite biological protein enzyme according to claim 1, is characterized in that, the preparation method of described composite biological protein enzyme is following steps:

Step 1, according to rice, millet, wheat, buckwheat, Chinese sorghum, soya bean, mung bean, Semen sesami nigrum, the corn of weight portion selection high-quality, and it is fully mixed, obtain cereal materials for subsequent use;

Step 2, according to weight portion edible sugar and edible brown sugar or wherein a kind of, with drinking water, fully stir, obtain syrup, for subsequent use;

Step 3, the cereal materials that step 1 and step 2 are obtained fully mix with syrup, are placed in airtight container spontaneous fermentation under normal temperature environment; After filtration, obtain cereal materials fermented liquid, for subsequent use;

Step 4, according to the fleece-flower root, dandelion, pseudo-ginseng, dried orange peel, Poria cocos, Chinese yam, the bark of eucommia, matrimony vine, Radix Angelicae Sinensis, lily, the tealeaves of weight portion selection high-quality, and it is fully mixed, obtain traditional Chinese medicinal material raw materials, for subsequent use;

Step 5, according to weight portion edible sugar and edible brown sugar or wherein a kind of, with drinking water, fully stir, obtain syrup, for subsequent use;

Step 6, the traditional Chinese medicinal material raw materials that step 4 and step 5 are obtained fully mix with syrup, are placed in airtight container spontaneous fermentation under normal temperature environment; After filtration, obtain traditional Chinese medicinal material raw materials fermented liquid, for subsequent use;

Step 7, the cereal materials fermented liquid that step 3 and step 6 are obtained fully mix with traditional Chinese medicinal material raw materials fermented liquid, are placed in airtight container spontaneous fermentation under normal temperature environment; After filtration, obtain composite biological protein enzyme; Obtain composite biological protein enzyme carry out canned, obtain oral liquid formulations.