CN105582261A - Traditional Chinese medicine composition with antioxidant function and preparation method thereof - Google Patents

Traditional Chinese medicine composition with antioxidant function and preparation method thereof Download PDF

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Publication number
CN105582261A
CN105582261A CN201610097203.6A CN201610097203A CN105582261A CN 105582261 A CN105582261 A CN 105582261A CN 201610097203 A CN201610097203 A CN 201610097203A CN 105582261 A CN105582261 A CN 105582261A
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root
chinese medicine
medicine composition
traditional chinese
parts
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吴赛春
林艳英
钟杰敏
邓裕彦
杨良
刘冠萍
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Guangxi Wuzhou Pharmaceutical Group Co Ltd
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Guangxi Wuzhou Pharmaceutical Group Co Ltd
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/57—Magnoliaceae (Magnolia family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/21—Amaranthaceae (Amaranth family), e.g. pigweed, rockwort or globe amaranth
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
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    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/1605—Excipients; Inactive ingredients
    • A61K9/1629—Organic macromolecular compounds
    • A61K9/1652—Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
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    • A61K9/2022—Organic macromolecular compounds
    • A61K9/205—Polysaccharides, e.g. alginate, gums; Cyclodextrin
    • A61K9/2059—Starch, including chemically or physically modified derivatives; Amylose; Amylopectin; Dextrin
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    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps

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Abstract

The invention discloses a traditional Chinese medicine composition with an antioxidant function and a preparation method thereof. The composition is prepared from the raw materials radix kadsurea coccineae, achyranthes bidentata, herba artemisiae anomalae, smilax riparia, radix cudramiae, cassia twig and fruit of largeflower gardenia. The results of animal pharmacodynamic experiments indicate that in an antioxidant function experiment, the traditional Chinese medicine composition can effectively improve the activity of GSH-Px in mice suffering hepatotoxicity caused by bromobenzene and reduce the content of lipid peroxidation end product MDA; and the effect of the embodiment group in clearing superoxide anions is obviously better than that of the control group (P<0.01). The traditional Chinese medicine composition disclosed by the invention can be regarded to have an antioxidant effect on the mice suffering hepatotoxicity, and the results of in-vitro experiments show an in-vitro antioxidant effect of the traditional Chinese medicine composition disclosed by the invention.

Description

A kind of Chinese medicine composition with anti-oxidation function and preparation method thereof
Technical field
The present invention relates to pharmaceutical sanitary field, be specifically related to a kind of Chinese medicine composition with anti-oxidation function andPreparation method.
Background technology
Oxidation is the biggest threat of skin aging, and diet is unhealthy, and Exposure to Sunlight, pressure, environmental pollution etc. can allowSkin free radical spreads unchecked, thereby produces the oxidative phenomenas such as complexion dimness, lack of water. It is all " the crime that health produces oxidationChief chief culprit ". So no matter from healthy aspect or from skin care aspect, we need to note in daily lifeAnti-oxidant. The polyphenoils of human body has self synthetic, also has by food supply. Enzyme and non-enzyme are anti-oxidantMaterial plays vital effect in protecting due to kinetic peroxide injury. Supplementary polyphenoils hasBe beneficial to Exercise Mechanics and reduce the generation of free radical or accelerate it and remove, with the side effect to Green Tea Extract, thereby rightCommon people and athletic health are all useful, and possible delayed motion fatigue occurs and quickening physical efficiency recovery.
In the physiological metabolism process of human normal, can produce free radical, under normal circumstances, the generation of interior free ylWith remove in poised state. With advancing age, antioxidant reductase is as superoxide dismutase (SOD) etc.Activity decline, the ability of removing free radical in body is declined, in body, excessive free radical will cause lipid mistakeOxidation, damage biomembrane, affect cell function, cause metabolic disorder, accelerate old and feeble with cause numerous diseaseOccur. Known free radical is one of major reason causing aging and many diseases, according to estimates about 80%-90%Aging, degenerative disorders all relevant with free radical, and for example common cancer, artery sclerosis, diabetes,Cataract, cardiovascular disease, arthritis etc., it is relevant to free radical that these diseases are all considered to. So eliminate harmfulRadical pair most important in health and the young physique of health. Therefore, by supplementing anti-oxidant nutrimentContribute to the prophylactic treatment to these diseases. Although the kind of listing is more at present, the mechanism of action is also had any different,Still can not meet all kinds of crowds' daily life needs.
Chinese patent application CN201110034083.2 disclose a kind of American Ginseng, glossy ganoderma, grape pip, rhodiola root,The Chinese medicine composition of auxiliary material composition, its proportioning is American Ginseng 0%～30%, glossy ganoderma 0%～30%, grape pip0%～30%, rhodiola root 0%～30%, auxiliary material 0%～50%. There is invigorating qi for tranquilization, qi and activate blood circulation, gasCloudy giving young employees remedial-courses in general knowledge and vocational skills, several compositions in formula share the effect that can jointly reach anti-oxidant, delay senility.
Relevant Chinese medicine composition is less for report anti-oxidant, that remove free radical at present, for this situation, sends outA person of good sense is through a large amount of tests, finally determines and proposes the present invention.
Summary of the invention
The object of this invention is to provide a kind of Chinese medicine composition with anti-oxidation function;
Another object of the present invention is to provide a kind of preparation method of the Chinese medicine composition with anti-oxidation function.
The object of the invention is to realize by following technical scheme:
The Chinese medicine composition with anti-oxidation function of the present invention is by the raw material preparation of following weight portionBecome: kadsura root 135-170 part, root of bidentate achyranthes 70-82 part, Artemisia anomala 70-82 part, riparian greenbrier root and rhizome 70-82 part,Cudrania root 70-82 part, cassia twig 13-18 part, build Cape jasmine 70-82 part.
Preferably, be to be prepared from by the raw material of following weight portion: 153 parts of kadsura roots, 76 parts of the roots of bidentate achyranthes,Artemisia anomala 76,76 parts of riparian greenbrier root and rhizomes, 76 parts of cudrania roots, 15 parts of cassia twig, build 76 parts of Cape jasmines.
Below crude drug source of the present invention:
Kadsura root: be the thick leaf fruit of Chinese magnoliavine of Magnoliacea plant Kadsuracoccinea (Lem.) A.C.SmithDry root. Record in " Chinese pharmacopoeia " version in 1977. Or caulis piperis futokadsurae, i.e. Kadsura heteroclita KadsuraThe dry rattan of heteroclita (Roxb.) Craib, records in " Guangxi Chinese medicine standard ". [nature and flavor] sweet,Micro-pungent, temperature. [function with cure mainly] expelling wind and clearing away cold, promoting qi circulation and relieving pain, stimulates the circulation of the blood and cause the muscles and joints to relax. For rheumatic arthritis,Lumbar muscle strain, flu, postpartum paralysis.
The root of bidentate achyranthes: be the dry root of amaranthaceous plant root of bidentate achyranthes AchyranthesbidentataBl.. When winter, cauline leaf was witheredExcavate, remove fibrous root and silt, be bundled into wisp, shine as for after wrinkle, top is trimmed, dry. [nature and flavor withGui Jing] bitter, sweet, sour, flat. Return liver, kidney channel. [function with cure mainly] stimulates the menstrual flow by the stasis of blood, filling liver kidney, strong muscleBone, inducing diuresis for treating strangurtia, ensurings proper downward flow of the blood. Be used for through closing, dysmenorrhoea, soreness of waist and knee joint, muscles and bones is unable, stranguria, oedema,Headache, dizzy, toothache, day sore, spits blood, bleeding from five sense organs or subcutaneous tissue.
Artemisia anomala: this product is feverfew artemisia anomalas ArtemisiaanomalaS.Moore or white luxuriant wormwood artemisia ArtemisiaActifloraWall.ex.DC. dry aerial parts. When summer, fall flowering, tap, remove impurity, dry.[nature and flavor] micro-hardship, pungent, temperature. [function with cure mainly] promoting blood circulation to remove blood stasis, inducing meastruation to relieve menalgia, inducing diuresis and reducing edema, disappear long-pendingDe-swelling. For abdominalgia with blood stasis in postpartum, irregular menstruation, amenorrhoea, dyspeptic abdominal distention, traumatic injury.
Riparian greenbrier root and rhizome: the dry root machine rhizome of Smilacaceae plant riparian greenbrier root and rhizome SmilaxripariaA.DC.. Summer, autumnExcavate, remove rattan and silt, dry. [nature and flavor] are sweet, bitter, flat. [function with cure mainly] channels sootheing and network vessel quickening,Inrigorating qi and promoting blood circulation, expelling phlegm and arresting coughing. For arthralgia and myalgia, deficiency of vital energy edema, traumatic injury, cough is spitted blood.
Cudrania root: this product is moraceae plants cudrania cochinchinensis Cudraniacochinchinensis (Lour.) KudoetMasam.Dry root. All can excavate the whole year, removes fibrous root, clean, section or section, dry. [nature and flavor] micro-hardship,Cool. [function with cure mainly] cough-relieving, removing jaundice, invigorates blood circulation, dredging collateral. For pulmonary tuberculosis, jaundice with damp-heat pathogen, side of body rib painBitterly, fall and flutter stasis of blood pain, arthralgia due to wind-dampness.
Cassia twig: be the dry spray of canella Chinese cassia tree CinnamomumcassiaPresl. Spring, Xia ErjiGather, except defoliation, dry, or section is dried. [nature and flavor] acrid, sweet, warm. The thoughts of returning home, lung, urinary bladder channel.[function with cure mainly] sweating expelling pathogenic factors from muscles and skin, warming meridian, helps yang transforming qi, the flat gas that spins. For anemofrigid cold, gastral cavityAbdomen crymodynia, blood is cold through closing, joint pain, phlegm and retained fluid, oedema, palpitaition, globefish runs quickly.
Build Cape jasmine: be madder wort great Hua mast GardeniajasminoidesEllisvar.grandillora (Lour.)The dry mature fruit of Nakai. Collecting fruit about October, cuts off the calyx head of depositing place, after drying or boilingDry or dry. [nature and flavor] hardship, cold. [function with cure mainly] subsides a swelling active. Be used for controlling injury from falling down bruise,Skin bruise pain.
Another aspect of the present invention has been to provide the preparation method of active component of the present invention, and the method is to adopt waterCarry or the alcohol extract of 40-80% concentration expressed in percentage by volume. Concrete preparation method is as follows:
Get kadsura root, the root of bidentate achyranthes, Artemisia anomala, riparian greenbrier root and rhizome, cudrania root, cassia twig, build Cape jasmine, extracting in water 2-3Inferior, each amount of water is equivalent to 6-12 times of medicinal material gross weight, and each extraction time is 1-3 hour, mergesExtract, filters, and the clear cream that when filtrate is concentrated into 60-80 DEG C, relative density is 1.05-1.20, to obtain final product.
The Chinese medicine composition with anti-oxidation function of the present invention, can with pharmaceutically acceptable carrierCoordinate, make various common dosage forms, as tablet, granule, capsule, chewable tablets, pill etc.
Pharmaceutically acceptable carrier of the present invention includes but not limited to following:
Diluent: starch, Icing Sugar, lactose, dextrin, microcrystalline cellulose, inorganic salts, glycitols etc.
Wetting agent and adhesive: purified water, ethanol, gelatin, polyethylene glycol, cellulose derivative etc.
Disintegrant: starch, sodium carboxymethyl starch, cellulose derivative, PVPP etc.
Lubricant: dolomol, superfine silica gel powder, talcum powder, polyethylene glycol etc.
Cosolvent: water, ethanol, glycerine, propane diols, liquid paraffin, plant wet goods.
Flavouring: sucrose, monose, aromatic etc.
Anticorrisive agent: benzoic acid, sorbic acid, methyl esters, ethyl ester, propyl ester etc.
Method 1: granule
The active component of getting scheme one to three gained, is dried to dried cream powder, mixes, and adds granule conventionalAuxiliary material, production method is prepared into granule of the present invention routinely.
The conventional auxiliary material of above-mentioned granule comprises one of diluent, wetting agent, adhesive or whole.
Method 2: tablet
The active component of getting scheme one to three gained, is dried to dried cream powder, mixes, and adds tablet conventional auxiliaryMaterial, production method is prepared into tablet of the present invention routinely.
The conventional auxiliary material of above-mentioned tablet comprises one of diluent, wetting agent, adhesive, disintegrant, lubricant or completePortion.
Method 3: capsule
The active component of getting scheme one to three gained, is dried to dried cream powder, mixes, and adds capsule conventionalAuxiliary material, production method is prepared into capsule of the present invention routinely.
The conventional auxiliary material of above-mentioned capsule comprises one of diluent, wetting agent, adhesive, lubricant or whole.
Method 4: chewable tablets
The active component of getting scheme one to three gained, is dried to dried cream powder, mixes, and adds chewable tablets conventionalAuxiliary material, production method is prepared into chewable tablets of the present invention routinely.
The conventional auxiliary material of above-mentioned chewable tablets comprise one of diluent, wetting agent, adhesive, disintegrant, lubricant orAll.
Method 5: pill
The active component of getting scheme one to three gained, is dried to dried cream powder, mixes, and adds pill conventional auxiliaryMaterial, production method is prepared into pill of the present invention routinely.
The conventional auxiliary material of above-mentioned pill comprises one of diluent, wetting agent, adhesive, lubricant or whole.
Fang Xie: body blockage of main and collateral channels, stagnation of QI-blood, causes function impaired, and oxygen radical is produced and increase,And then damage again internal organs channels and collaterals, accelerate human senescence. We are with promoting blood circulation and removing blood stasis, and promoting the circulation of qi is the main rules for the treatment of. Fang ZhongTo reuse kadsura root as monarch, kadsura root taste is pungent, warm in nature, and benefaction meridian qi and blood and temperature are supported passages through which vital energy circulates, and research is sent outNow, in kadsura root, there is the oxidation-resistant active ingredient of methyl shikimate, syringaldehyde; We's minister is with the root of bidentate achyranthes, LiuPost slave, riparian greenbrier root and rhizome, root of bidentate achyranthes merit is kind stimulates the menstrual flow by the stasis of blood, and can principal drug assistance promoting the circulation of qi blood stasis stagnant, research be found, InokopolyoseThere is significant antioxidation activity with total saponin(e; Artemisia anomala fragrant odour, kind broken blood stimulates the menstrual flow, and blood stasis removing analgesic, helpsThe capable medicine gesture of monarch drug in a prescription; Riparian greenbrier root and rhizome nature and flavor are sweet, and flat, the tonifying Qi of can invigorating blood circulation, can regulate channels and activate collateral again, and two arrogate to oneself its merit, grindStudy carefully discovery, the hydroxycinnamic acid that contains in riparian greenbrier root and rhizome, hydroxycinnamic acid methyl esters, Ferulic acid methylester etc. haveSignificant antioxidation activity. Fang Zhongzuo is with cudrania root and build Cape jasmine, and cudrania root energy promoting blood circulation by removing wind, regulates channels and activates collateral, canCleanse blood vessels and qi and blood circulation promotion; Cassia twig merit is apt to warming meridian, can the vigorous passages through which vital energy circulates qi and blood of principal drug assistance, and cassia twig contains transThe oxidation-resistant active ingredient such as cinnamic acid, o-methoxy cinnamic aldehyde, the alcohol extract of cassia twig has good removing freelyBase activity; Build Cape jasmine nature and flavor bitter cold, kind detumescence is active, and Determination of Gardenoside that research finds to build Cape jasmine is about cape jasmine2.14 times, Gardenoside has good anti-oxidant, anti-aging effects. All medicines share above, and current qi and blood, canFunction is improved, reduce the generation of body free radical, in side, many flavour of a drug have antioxidation activity,Can directly reduce the damage of free radical or Green Tea Extract.
A kind of Chinese medicine composition with anti-oxidation function provided by the invention has the following advantages:
1, animal pharmacodynamics experimental result shows: in anti-oxidation function experiment, the present invention can effectively improve bromineBenzene causes the GSH-Px activity in hepatotoxicity Mice Body, reduces the content of end product of lipid hyperoxygen MDA;The present invention removes superoxide anion has effect to be significantly better than comparative example (P < 0.01).
2, the present invention, in vitro in anti-oxidant experiment, has obvious scavenging action to superoxide anion. In addition originallyInvention has stronger inside and outside antioxidation, and effect is better than prior art.
3, present composition formula is rationally innovated, and production cost is low, technique is simple.
Detailed description of the invention
Further illustrate the present invention below by embodiment. It should be understood that embodiments of the invention be forThe present invention instead of limitation of the present invention are described. The simple modifications that essence according to the present invention is carried out the present inventionAll belong to the scope of protection of present invention.
Embodiment 1
Get kadsura root 135g, root of bidentate achyranthes 70g, Artemisia anomala 70, riparian greenbrier root and rhizome 70g, cudrania root 70g, cassia twig 13g,Build Cape jasmine 70g, extracting in water 3 times, each amount of water is equivalent to 6 times of medicinal material gross weight, each extraction timeBe 1 hour, merge extract, filter, the clear cream that when filtrate is concentrated into 60 DEG C, relative density is 1.20, dryDry one-tenth dried cream powder, pulverizes, and adds supplementary product starch, cane sugar powder, talcum powder, dolomol, makes granule.
Embodiment 2
Get kadsura root 170g, root of bidentate achyranthes 82g, Artemisia anomala 82, riparian greenbrier root and rhizome 82g, cudrania root 82g, cassia twig 18g,Build Cape jasmine 82g, extracting in water 2 times, each amount of water is equivalent to 10 times of medicinal material gross weight, while extraction at every turnBetween be 1.5 hours, merge extract, filter, the clear cream that when filtrate is concentrated into 70 DEG C, relative density is 1.15,Be dried to dried cream powder, pulverize, then add microcrystalline cellulose excipients, hydroxypropyl cellulose, sodium carboxymethyl starch,Talcum powder, dolomol are made tablet.
Embodiment 3
Get kadsura root 153g, root of bidentate achyranthes 76g, Artemisia anomala 76, riparian greenbrier root and rhizome 76g, cudrania root 76g, cassia twig 15g,Build Cape jasmine 76g, extracting in water 3 times, each amount of water is equivalent to 12 times of medicinal material gross weight, while extraction at every turnBetween be 2 hours, merge extract, filter, the clear cream that when filtrate is concentrated into 80 DEG C, relative density is 1.10,Be dried to dried cream powder, pulverize,, then add auxiliary material honey, beeswax to make pill.
Pharmacodynamics test:
One, interior antioxidation action
1. experiment material
1.1 animal
SPF level Kunming mouse, male, body weight 18～22g, in Guangxi Medical University medical experiment animalThe heart provides, animal production licence SCXK osmanthus 2003-0003.
1.2 medicines and reagent
Embodiment of the present invention 1-3. Vitamin E soft capsule, lot number 141016, Qingdao Double Whale Pharmaceutical Co., Ltd..MDA kit, lot number 150227, bio tech ltd is built up in Nanjing. GSH-Px kit, batchNumbers 150124, bio tech ltd is built up in Nanjing.
2. experimental technique
Select 60 of healthy kunming mices, male, be divided at random 6 groups, 10 every group, be respectively blank rightAccording to group, model group, embodiment group (50mg/kg), comparative example group (vitamin E 45mg/kg). Each group is littleEqual gastric infusion after mouse adaptability raising 5d, 1 time/d, administration volume is 10ml/kg, successive administration 14d,Mouse overnight fasting before last administration is respectively organized equal gavage except blank group after last administration 0.5～1h0.3mg/kg bromobenzene oil solution, 10ml/kg, puts to death animal after 20h, get hepatic tissue and make 5% LH, 4 DEG CThe centrifugal 15min of lower 4000rpm, separates liver tissue homogenate liquid supernatant, by kit description measure MDA withThe content of GSH-Px. All experimental datas all adopt SPSS13.0 statistical software to carry out variance analysis and groupBetween inspection.
3. experimental result is in table 1.
MDA in the each group of table 1 murine liver tissue, the content of GSH-Px
Note: with the comparison of blank group, * P < 0.05, * * P < 0.01; With model control group comparison, ☆ P < 0.05,☆ ☆ P < 0.01; With the comparison of comparative example group, △ P < 0.05, △ △ P < 0.01;
4. discuss
Table 1 result shows: compare with blank group, the MDA content in model group mouse liver is remarkableRising (P < 0.01), GSH-Px vigor significantly reduce (P < 0.01), and modeling success is described; With model groupRelatively, comparative example group, 1,2,3 groups of MDA content of embodiment all significantly reduce (P < 0.01), comparative example1 group of GSH-Px vigor of group and embodiment all reduces (P < 0.05), the GSH-Px of embodiment 2 and embodiment 3Vigor reduces significantly (P < 0.01); Compare with comparative example, 1 group of MDA of embodiment, the equal nothing of GSH-Px valueSignificant difference (P > 0.05), 2 groups of MDA content of embodiment significantly reduce (P < 0.05), and GSH-Px containsAmount significantly raises (P < 0.05), and wherein 3 groups of MDA content of embodiment significantly reduce (P < 0.01), GSH-PxContent significantly raise (P < 0.01).
Experimental result shows: the present invention can effectively improve bromobenzene and cause the GSH-Px activity in hepatotoxicity Mice Body,The content that reduces end product of lipid hyperoxygen MDA, has certain antioxidation, and effect is better than existing skillArt.
Two, Antioxidation in vitro
1. experiment material
1.1 medicine
Embodiment of the present invention 1-3, natural complex C, lot number 141203, the limited public affairs of Hainan health hall health productsDepartment.
1.2 reagent and instrument
Xanthine, xanthine oxidase, NBT (NBT), manufacturer is sigma.Potassium dihydrogen phosphate, potassium hydroxide, be domestic AR.
Ultraviolet specrophotometer, model UV2310 II, Shanghai Techcomp Instrument Ltd..
2. experiment grouping
Blank group: add distilled water
1 group of embodiment: add embodiment 1 liquid, by crude drug amount, 10mg/ml.
2 groups of embodiment: add embodiment 2 liquids, by crude drug amount, 10mg/ml.
3 groups of embodiment: add embodiment 3 liquids, by crude drug amount, 10mg/ml.
Contrast groups: give vitamin C liquid, 10mg/ml.
Below respectively organize liquid all with distilled water preparation, every group arranges 5 repetitions.
3. experimental technique:
At the KH of the 10mmol/l of 100 μ l2PO4In-KOH buffer solution (PH=7.4), add respective concentrationMedicine 50 μ l, 100 μ l100mol/l xanthine and 100 μ l0.02U/ml xanthine oxidases (through high pressureSterilizing), add 100mol/l NBT 100 μ l, under 25 DEG C of conditions, hatch 5min, in 550nm placeMeasure absorbance, calculate clearance rate=(△ OD blank-△ OD sample)/△ OD blank × 100%,△ OD=OD0min-OD5min. Obtained experimental data all adopts SPSS13.0 statistical software to carry out varianceBetween analysis and group, check.
4. experimental result is in table 2
The scavenging action of the each embodiment of table 2 to superoxide anion
Note: with the comparison of blank group, * P < 0.05, * * P < 0.01; With the comparison of comparative example group, △ P < 0.05,△△P＜0.01。
5. discuss
As seen from the results in Table 2, compare with blank group, comparative example and each embodiment group are all to super oxygen the moonIon has obvious scavenging action (P < 0.01). Compare with comparative example group, embodiment 1 is without significant difference,2 groups of significant differences of embodiment (P < 0.05), 3 groups of differences of embodiment are (P < 0.01) very significantly. Embodiment2 and embodiment 3 clearance rates all higher than comparative example group, illustrate that the embodiment of the present invention is better than prior art.
Conclusion: the present invention shows certain antioxidation in antioxidation in vitro experiment in vivo,
Although, above use general explanation, detailed description of the invention and test, the present invention is done detailedDescription, but on basis of the present invention, can make some modifications or improvements it, this is to those skilled in the artApparent. Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention,All belong to the scope of protection of present invention.

Claims (5)

1. there is a Chinese medicine composition for anti-oxidation function, it is characterized in that it is to be prepared from by the raw material of following weight portion: kadsura root 135-170 part, root of bidentate achyranthes 70-82 part, Artemisia anomala 70-82 part, riparian greenbrier root and rhizome 70-82 part, cudrania root 70-82 part, cassia twig 13-18 part, build Cape jasmine 70-82 part.
2. the Chinese medicine composition with anti-oxidation function as claimed in claim 1, is characterized in that it is to be prepared from by the raw material of following weight portion: 153 parts of kadsura roots, 76 parts of the roots of bidentate achyranthes, Artemisia anomala 76,76 parts of riparian greenbrier root and rhizomes, 76 parts of cudrania roots, 15 parts of cassia twig, build 76 parts of Cape jasmines.
3. the Chinese medicine composition with anti-oxidation function as claimed in claim 1 or 2, is characterized in that its active component is to adopt the alcohol extract of water extraction or 40-80% concentration expressed in percentage by volume to be prepared from.
4. the Chinese medicine composition with anti-oxidation function as claimed in claim 3, it is characterized in that its active component is prepared from by the following method: get kadsura root, the root of bidentate achyranthes, Artemisia anomala, riparian greenbrier root and rhizome, cudrania root, cassia twig, build Cape jasmine, extracting in water 2-3 time, each amount of water is equivalent to 6-12 times of medicinal material gross weight, each extraction time is 1-3 hour, merges extract, filters, the clear cream that when filtrate is concentrated into 60-80 DEG C, relative density is 1.05-1.20, to obtain final product.
5. there is a Chinese medicine preparation for anti-oxidation function, prepare and form by acceptable carrier in the Chinese medicine composition with anti-oxidation function described in claim 1 or 2 and pharmaceuticals industry.
CN201610097203.6A 2016-02-22 2016-02-22 Traditional Chinese medicine composition with antioxidant function and preparation method thereof Pending CN105582261A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101496603A (en) * 2009-02-19 2009-08-05 南方李锦记有限公司 Health-care food with enhancing immunity and antioxidation functions
CN102100810A (en) * 2011-02-01 2011-06-22 完美（中国）有限公司 Traditional Chinese medicine composition with antioxidant function and preparation method thereof
CN102198195A (en) * 2011-05-25 2011-09-28 遵义陆圣康源科技开发有限责任公司 Antioxidative medicinal composition

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101496603A (en) * 2009-02-19 2009-08-05 南方李锦记有限公司 Health-care food with enhancing immunity and antioxidation functions
CN102100810A (en) * 2011-02-01 2011-06-22 完美（中国）有限公司 Traditional Chinese medicine composition with antioxidant function and preparation method thereof
CN102198195A (en) * 2011-05-25 2011-09-28 遵义陆圣康源科技开发有限责任公司 Antioxidative medicinal composition

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