CN105581031A - Feed for treating fatty liver disease of turtles and preparation method thereof - Google Patents

Feed for treating fatty liver disease of turtles and preparation method thereof Download PDF

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CN105581031A
CN105581031A CN201510960315.5A CN201510960315A CN105581031A CN 105581031 A CN105581031 A CN 105581031A CN 201510960315 A CN201510960315 A CN 201510960315A CN 105581031 A CN105581031 A CN 105581031A
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parts
feed
fatty liver
liver disease
testudinate
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张莘蔓
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Abstract

The invention discloses a feed for treating a fatty liver disease of turtles and a preparation method thereof, and belongs to the technical field of turtle feed processing. The feed is prepared from, by weight, 30-50 parts of silkworm pupa meal, 50-70 parts of manihot utilissima powder, 8-16 parts of table salt, 6-14 parts of dimethyl-beta-propiothetin, 5-9 parts of betaine, 4-8 parts of glycine, 4-8 parts of cytidylic acid, 0.2-0.6 part of Tomatin polypeptide, 1-3 parts of citronellal and 15-42 parts of traditional Chinese medicine preparation. The feed is prepared through the steps of drying, smashing, screening, liquid medicine extracting, concentrating, mixing, pelletizing, sterilizing and the like. According to the feed for treating the fatty liver disease of the turtles, the effects of multiple traditional Chinese medicines are synthesized, the effects of resisting bacteria, diminishing inflammation, enhancing the body immune function and the like can be achieved, the cure rate of the fatty liver disease of the turtles can be effectively increased, and the death rate of the fatty liver disease of the turtles can be effectively decreased; the feed is reasonable in preparation, rich in nutrition and capable of increasing the feed intake of the turtles suffering from the fatty liver disease and increasing the growth speed.

Description

A kind of feed for the treatment of testudinate fatty liver disease and preparation method thereof
Technical field
The invention belongs to Terrapin feed processing technique field, be specifically related to a kind of feed and preparation thereof for the treatment of testudinate fatty liver diseaseMethod.
Background technology
It is edible, medicinal and the value such as view and admire that terrapin has. Terrapin is always considered to senior delicacies, is China's aquacultureImportant Economic kind is again excellent tonic product simultaneously, has certain effect to cancer and to improving human motion function. Along with peopleThe raising of life and the level of consumption, people are more and more higher to the demand of testudinate, only depend on wild testudinate resource, can not meet day far awayBenefit increase consumer need. Under the driving of the market demand and radical economic benefit, increasing raiser starts to send out at homeTestudinate is propagated in exhibition artificially.
Under normal circumstances, liver constantly synthesizes triacylglycerol (TC) by free aliphatic acid, more defeated with the form of lipoproteinIn blood. If hyper acid for blood Free Fat, in liver, in the synthetic increase of triacylglycerol or liver, lipoprotein discharge reduces, this non-Dynamic equilibrium is controlled in time and is reversed as can not get, and As time goes on will form fat hepatopathy. So cause testudinate fat liver propertyThe main cause of liver pathological changes be throw something and feed for a long time lack methionine, cystine in high fat, high cholesterol or albumen feed institute extremely. TortoiseOnce soft-shelled turtle suffers from fatty liver disease, easily causes the testudinate death rate high, causes raiser to suffer huge loss. Therefore, having very much mustResearch and develop a kind of feed that is used for the treatment of testudinate fatty liver disease.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of feed for the treatment of testudinate fatty liver disease and preparation method thereof, to solveTestudinate suffers from fatty liver disease and causes the problems such as the speed of growth is slow, the death rate is high, cultivation has a big risk, economic benefit is low. The present invention combinesClose plurality of Chinese effect, can play the effect such as anti-inflammation, enhanced machine body immunity function, can effectively improve testudinate and suffer from fatThe cure rate of hepatopathy and the reduction death rate; Feed preparation of the present invention is reasonable, nutritious, can increase and suffer from fatty liver disease tortoiseSoft-shelled turtle feed intake, improves the speed of growth.
For solving above technical problem, the present invention by the following technical solutions:
Treat a feed for testudinate fatty liver disease, taking weight portion as unit, comprise following raw material: dried silkworm chrysalis meal 30-50 part,Tapioca starch 50-70 part, salt 8-16 part, dimethyl-β-DMPT 6-14 part, betaine 5-9 part, glycine 4-8Part, cytidylic acid 4-8 part, holder Martin polypeptide 0.2-0.6 part, citronellal 1-3 part, Chinese medicine preparation 15-42 part, instituteState the raw material that Chinese medicine preparation comprises following weight portion: Sophora alopecuroide 1-3 part, mistletoe 1-3 part, bark of ash 1-3 part, Radix Isatidis 1-3Part, wild marjoram 1-3 part, plantain seed 0.8-2 part, bitter dish 0.8-2 part, herba aristolochiae 0.8-2 part, Radix Astragali 0.8-2 part, Patrinia scaniosaefoliaGrass 0.8-2 part, root of Dahurain angelica 0.6-1.6 part, root of kudzu vine 0.6-1.6 part, dandelion 0.6-1.6 part, Chinese lobelia 0.6-1.6 part,Herba Siegesbeckiae 0.6-1.6 part, honeysuckle 0.4-1.2 part, bark of eucommia 0.4-1.2 part, radix bardanae 0.4-1.2 part, lily 0.4-1.2Part, earthworm 0.4-1.2 part, cordate houttuynia 0.2-0.6 part, achene of Siberian cocklebur 0.2-0.6 part, wild aconite root 0.2-0.6 part, sweet wormwood 0.2-0.6Part, asafoetide 0.2-0.6 part.
Preferably, the feed of described treatment testudinate fatty liver disease, taking weight portion as unit, comprises following raw material: dried silkworm chrysalis meal35-45 part, tapioca starch 55-65 part, salt 10-14 part, dimethyl-β-DMPT 8-12 part, betaine 6-8 part,Glycine 5-7 part, cytidylic acid 5-7 part, holder Martin polypeptide 0.3-0.5 part, citronellal 1.5-2.5 part, Chinese medicine systemAgent 21-36 part, described Chinese medicine preparation comprises the raw material of following weight portion: Sophora alopecuroide 1.5-2.5 part, mistletoe 1.5-2.5 part,Bark of ash 1.5-2.5 part, Radix Isatidis 1.5-2.5 part, wild marjoram 1.5-2.5 part, plantain seed 1-1.8 part, bitter dish 1-1.8 part,Herba aristolochiae 1-1.8 part, Radix Astragali 1-1.8 part, field pennycress 1-1.8 part, root of Dahurain angelica 0.8-1.4 part, root of kudzu vine 0.8-1.4 part, PuPublic English 0.8-1.4 part, Chinese lobelia 0.8-1.4 part, Herba Siegesbeckiae 0.8-1.4 part, honeysuckle 0.6-1 part, bark of eucommia 0.6-1 part,Radix bardanae 0.6-1 part, lily 0.6-1 part, earthworm 0.6-1 part, cordate houttuynia 0.3-0.5 part, achene of Siberian cocklebur 0.3-0.5 part,Wild aconite root 0.3-0.5 part, sweet wormwood 0.3-0.5 part, asafoetide 0.3-0.5 part.
Preferably, the feed of described treatment testudinate fatty liver disease, taking weight portion as unit, comprises following raw material: dried silkworm chrysalis meal40 parts, 60 parts of tapioca starch, 12 parts of salt, 12 parts of dimethyl-β-DMPTs, 8 parts of betaines, 6 parts of glycine,6 parts of cytidylic acids, 0.4 part of holder Martin polypeptide, 2 parts of citronellals, 28.5 parts of Chinese medicine preparations, described Chinese medicine preparation comprisesThe raw material of following weight portion: 2 parts of Sophora alopecuroides, 2 parts of mistletoes, 2 parts of the barks of ash, 2 parts of Radix Isatidis, 2 parts of wild marjorams, plantain seed1.5 parts, 1.5 parts of bitter dishes, 1.5 parts of herba aristolochiaes, 1.5 parts of the Radixs Astragali, 1.5 parts of field pennycress, 1 part of the root of Dahurain angelica, 1 part of the root of kudzu vine,1 part of dandelion, 1 part of Chinese lobelia, 1 part of Herba Siegesbeckiae, 0.8 part of honeysuckle, 0.8 part of the bark of eucommia, 0.8 part of radix bardanae, lily0.8 part, 0.8 part of earthworm, 0.4 part of cordate houttuynia, 0.4 part of the achene of Siberian cocklebur, 0.4 part of wild aconite root, 0.4 part of sweet wormwood, asafoetide 0.4Part.
The present invention also provides a kind of preparation method of feed for the treatment of testudinate fatty liver disease, comprises the following steps:
S1: respectively silkworm chrysalis, cassava are dried and pulverized at 45-60 DEG C, excessively mix system after 150-250 object sieveObtain mixture A;
S2: by Sophora alopecuroide, mistletoe, the bark of ash, Radix Isatidis, wild marjoram, plantain seed, bitter dish, herba aristolochiae, the Radix Astragali, field pennycress,The root of Dahurain angelica, the root of kudzu vine, dandelion, Chinese lobelia, Herba Siegesbeckiae, honeysuckle, the bark of eucommia, radix bardanae, lily, earthworm, cordate houttuynia, greyThe ears or side handles of a utensil, wild aconite root, sweet wormwood, asafoetide co-grinding, obtain mixture B, mixture B joined in organic solvent, superSound wave assisted Extraction is followed the example of lower extraction liquid, and the medical filtration of gained, concentrated, obtains mixture C, the mixture C of gained is dry,Pulverize, sieve, sterilizing, make Chinese medicine preparation;
S3: the mixture A that step S1 is made, the Chinese medicine preparation that step S2 makes, salt, dimethyl-β-DMPT,Betaine, glycine, cytidylic acid, holder Martin polypeptide, citronellal, and 300-700 part water, mix again,Make feed crude product;
S4: the feed crude product that step S3 is made make diameter be the particle of 0.05-0.2cm and temperature be at 90-100 DEG C dryDoing to water content≤6%, is 135-136 DEG C in temperature, and pressure is under 220-230KPa, and sterilizing 2-3min makes treatment tortoiseThe feed of soft-shelled turtle fatty liver disease.
Preferably, organic solvent described in step S2 comprises one or more in ethanol, butanone, trichloroethanes, carbon disulfide.
Preferably, the condition that the assisted Extraction of ultrasonic wave described in step S2 is followed the example of extraction liquid is: ultrasonic power is 150-170W,Organic solvent concentration is 50%-80%, and extraction temperature is 50-70 DEG C, and extraction time is 30-50min.
Preferably, step S2 herb liquid filters, and is concentrated into water content 10%-22%.
Preferably, in step S2, mixture B is dried to water content 4%-6% at 95-105 DEG C.
The condition of preferably, sieving in step S2 is: cross 120-320 mesh sieve.
Preferably, in step S2, the condition of sterilizing is: temperature is 135-136 DEG C, and pressure is 220-230KPa, and the time is 1-2min.
It is as follows that component of the present invention has effect:
Dried silkworm chrysalis meal is the fine component of balanced ration amino acid composition, and its another feature is that arginine content is low, especially with relyingThe ratio of histidine content is very low, is suitable for and other feed compatibility, and calcium, the phosphorus content of dried silkworm chrysalis meal are lower, but B family vitamin containsAmount is abundant, and especially riboflavin content is higher.
Tapioca starch can be used as supplying raw material of Dietary Energy, can reduce the cost of feed, can improve the digestion of testudinate simultaneously and inhaleYield.
Salt is mineral matter important in feed, is the requisite material of the activity of sustaining life. Sodium can maintain blood and tissue fluidAcid-base balance, regulate the stable of normal osmotic pressure, secretion that can also saliva stimulating, promotes the appetite of testudinate. Sodium can impel fatThe organic matter such as fat and protein synthesizing in metabolic process.
Dimethyl-β-DMPT is a kind of phagostimulant, has methyl donor function, is effectively utilized by testudinate, promotes testudinateThe secretion of digestive ferment in body, promotes the digestion of testudinate and the absorption to nutriment, improves efficiency of feed utilization.
Betaine is a kind of desirable feeding additive aquatic animal, can improve the survival rate of young testudinate, promotes testudinate growth, can also be comparativelyStimulate well sense of smell and the sense of taste of testudinate, improve the palatability of feed, reduce feed waste, reduce feed coefficient, betaine alsoCan improve the meat of testudinate, reduce the fat content of testudinate.
Glycine has compared with strong stimulation effect the sense of taste and sense of smell, improves feed intake, mixed with cytidylic acid, produces collaborative luringFood benefit.
Holder Martin polypeptide is a kind of sweetener, can strengthen the sweet taste of feed, covers original bad taste in feed, improves testudinateFeed intake.
Citronellal is a kind of flavouring agent, can improve feed palatability, promotes testudinate appetite, the secreting function of the body of gland that stimulates digestion,Promote the growth of testudinate.
The total alkaloid of sophora alopecuroide extracting in Sophora alopecuroide has anti-inflammatory, anticancer, antiallergic action, anti-arrhythmia cordis, antiulcer, literHigh leukocytic, relieving asthma, antipyretic, desinsection, calmness, analgesia, the effect such as antiviral; The external application of Sophora alopecuroide powder to staphylococcus,Escherichia coli, streptococcus, fungi, bacterium protein of Gardnerella vaginalis and trichomonad have inhibition or killing action.
Mistletoe has wines used as antirheumatic, filling liver kidney, strengthening the bones and muscles, the effect such as antiabortive, can control headache, arthralgia due to wind-dampness, soreness and weakness of waist and knees,Fetal irritability, uterine bleeding are through the illnesss such as many.
The effects such as the bark of ash has heat-clearing and damp-drying drug, clears liver and improve vision, antiasthmatic-antitussive, can cure cold, the disease such as acute bronchitis, pneumoniaShape.
Radix Isatidis (conventional another name: indigo-blue, indigo root, manyflower glorybower root) is a kind of Chinese medicine, is that 1 year or biennial herb are plantedThe root of thing woaded blue, has anti-bacteria and anti-virus, anti-Leptospira, removing toxic substances, raising immunologic function, the effect such as antitumor.
Wild marjoram has another name called only dysentery grass, Elsholtzia patrini garcke, south Sinkiang ziziphora herb, another name: Ao Legang grass, E Ligangye, Pizza grass, mushroom grass.It is the strongest to staphylococcus aureus, typhoid bacillus, proteus antibacterial action, to Escherichia coli, bacterium sonnei and pairTyphoid bacillus also has good antibacterial action; Wild marjoram energy promotion health people and acute nephritis patient's lymph transformation, and haveImmunoregulation effect.
Plantain seed has another name called Asiatic plantain, five grass, wheel dish, cold in nature, taste is sweet, nontoxic, have clearing heat and detoxicating, diuresis excreting dampness,The effects such as improving eyesight of eliminating the phlegm, are used for the treatment of oedema, phlegm-heat cough, have loose bowels, heat drenches the diseases such as puckery pain, red eye, swell pain.
In bitter dish, contain the composition such as taraxasterol, choline, staphylococcus aureus resistance bacterial strain, hemolytic streptococcus are hadStrong bactericidal action, also has certain to Diplococcus pneumopniae, meningococcus, corynebacterium diphtheriae, Pseudomonas aeruginosa, shigella dysenteriae etc.Lethal effect, therefore equal to icterepatitis, sphagitis, bacillary dysentery, cold, fever and chronic bronchitis, tonsillitis etc.There is certain curative effect.
Herba aristolochiae hardship, temperature, have and declare the tunnel that stimulates the menstrual flow, dodar stasis, and soothing the liver promoting the circulation of qi, stops the effects such as heart stomachache.
The root of large-flowered skullcap has the effects such as heat-clearing and damp-drying drug, cool blood are antiabortive, removing toxic substances, cure mainly warm heat disease, the infection of the upper respiratory tract, cough with lung heat,The symptoms such as damp and hot jaundice, pneumonia, dysentery, hemoptysis, hot eyes, fetal irritability, hypertension, the swollen furuncle sore of carbuncle.
Field pennycress, with root-like stock and root, all herbal medicine, belongs to antipyretic and antidotal type medicine in Chinese medicine, cool in nature, taste is pungent, bitter. Return liver,Stomach, large intestine channel, have effect clearing heat and detoxicating, removing blood stasis and expelling pus. Modern pharmacy thinks, it containing volatile oil, Patrinia scaniosaefolia saponin and β-Sterol glucose formula, have anti-inflammatory, antibacterial, calm, protect the liver and the effect of cardiac stimulant diuresis. Field pennycress to staphylococcus aureus,Shigella dysenteriae, typhoid bacillus, Pseudomonas aeruginosa have inhibition or killing action.
The effects such as the root of Dahurain angelica has wines used as antirheumatic, the apocenosis of invigorating blood circulation, myogenic pain relieving, can control headache, toothache, nasosinusitis, intestines wind anal fistula, redUnder leukorrhea, the illness such as ulcer sores, pruitus.
The root of kudzu vine has relieving muscles to expel heat, delivers and examine, promote the production of body fluid to quench thirst, rise the effects such as positive antidiarrheal, can control fever caused by exogenous pathogens, rigidity of nape and headache,Measles goes out the symptoms such as smooth, warm disease is thirsty, diabete from the beginning of, rash.
The effects such as that dandelion has is clearing heat and detoxicating, the carbuncle that disappears dissipating bind, anti-inflammatory, cool blood, diuresis, cholagogic, can control the hot red eye, swell pain of liver,The symptoms such as purulent disease.
Chinese lobelia has inducing diuresis to reduce edema, the effect such as clearing heat and detoxicating, can control difficult urination, the edema of the face, snake bite and insect sting, cancer of the stomach intestinesThe illnesss such as cancer, the cancer of the esophagus, liver cancer.
Herba Siegesbeckiae has the effect such as wines used as antirheumatic, relieving rigidity of muscles and joints, can control quadriplegia, arthralgia and myalgia, waist-leg weakness, malaria, acuteThe illnesss such as hepatitis, high blood pressure, furuncle swelling toxin.
The effects such as that honeysuckle has is clearing heat and detoxicating, dispelling wind and heat from the body, cure mainly red and swollen heat pain, affection of exogenous wind-heat, warm disease from the beginning of, hot malicious bloodThe symptoms such as dysentery, hot summer weather polydipsia, abscess of throat.
The bark of eucommia have tonify the liver and kidney, strengthen muscles and bones, coordinating Chong and Conception Vessels, solid through the effect such as antiabortive, can control cough, soreness of waist and knee joint, sunThe symptoms such as impotence, seminal emission, frequent micturition.
Radix bardanae has the effects such as the heat of dispeling the wind, detumescence poison, can control that wind poison edema of the face, dizziness, throat heat are swollen, dentalgia, cough, disappearsThe symptom such as thirsty.
Lily has removing toxic substances, reason spleen stomach invigorating, dampness removing disappear long-pending, antitoxic heart-soothing and sedative, the effect such as stimulate circulation, and cures mainly labor and coughs, coughsThe symptoms such as blood, dysphoria palpitation with fear.
Earthworm has the effects such as heat-clearing, flat liver, Zhichuan, dredging collateral, cures mainly irritated, the frightened epilepsy tic of high heat, dyspnea and cough due to lung-heat, thermojunctionThe symptoms such as renal shutdown.
The effects such as that cordate houttuynia has is clearing heat and detoxicating, inducing diuresis to reduce edema, can cure cold, pneumonia, lung abscess, hot dysentery, malaria, oedema,The symptom such as gonorrhoea, leukorrhea.
The achene of Siberian cocklebur has the effects such as expelling cold, eliminating, clearing the nasal passage, wines used as antirheumatic, pain relieving, can control the diseases such as pain in waist and lower extremities, mumps, malariaDisease.
Wild aconite root have dispel the wind, dehumidify, the effect such as loose cold, pain relieving, can preventing and curing malaria disease, wind-cold-dampness arthralgia, arthralgia, Xin FulengBitterly, cold hernia have a pain, the illness such as narcotic analgesic.
Sweet wormwood has heat-clearing, relieving summer-heat, except effects such as steamings, can control warm disease, hot summer weather, hectic fever due to yin labor heat, malaria, dysentery, jaundice,The illness such as scabies, itch.
The effects such as that having of asafoetide disease disappears is long-pending, desinsection, preventing malaria, can control the diseases such as dyspepsia, fullness and oppression of chest and abdomen, crymodynia, malaria, dysenteryDisease.
The present invention has following beneficial effect:
(1) feed of the present invention combines Sophora alopecuroide, mistletoe, the bark of ash, Radix Isatidis, wild marjoram, plantain seed, bitter dish, beautyRattan, the Radix Astragali, field pennycress, the root of Dahurain angelica, the root of kudzu vine, dandelion, Chinese lobelia, Herba Siegesbeckiae, honeysuckle, the bark of eucommia, radix bardanae, lily,Earthworm, cordate houttuynia, the achene of Siberian cocklebur, wild aconite root, sweet wormwood, asafoetide plurality of Chinese effect, can play anti-inflammation, strengthen bodyThe effects such as immunologic function, it is good that testudinate absorbs this feed efficiency, can effectively reduce the death rate and the raising of testudinate trouble fatty liver disease and controlMore rate;
(2) feed of the present invention sterilizing under HTHP, short, effect of time, holding time are long;
(3) feed preparation of the present invention is reasonable, nutritious, can increase and suffer from fatty liver disease testudinate feed intake, improves growth speedDegree;
(4) feed producing method of the present invention is simple to operate, and production cost is low, is conducive to apply.
Detailed description of the invention
Be specifically described below in conjunction with specific embodiment.
Embodiment 1
Treat a feed for testudinate fatty liver disease, taking weight portion as unit, comprise following raw material: 30 parts of dried silkworm chrysalis meals, cassava50 parts, powder, 8 parts of salt, 6 parts of dimethyl-β-DMPTs, 5 parts of betaines, 4 parts of glycine, cytidylic acid 4Part, 0.2 part of holder Martin polypeptide, 1 part of citronellal, 15 parts of Chinese medicine preparations, described Chinese medicine preparation comprises the raw material of following weight portion:1 part of Sophora alopecuroide, 1 part of mistletoe, 1 part of the bark of ash, 1 part of Radix Isatidis, 1 part of wild marjoram, 0.8 part of plantain seed, 0.8 part of bitter dish,0.8 part of herba aristolochiae, 0.8 part of the Radix Astragali, 0.8 part of field pennycress, 0.6 part of the root of Dahurain angelica, 0.6 part of the root of kudzu vine, 0.6 part of dandelion, half0.6 part, limit lotus, 0.6 part of Herba Siegesbeckiae, 0.4 part of honeysuckle, 0.4 part of the bark of eucommia, 0.4 part of radix bardanae, 0.4 part of lily,0.4 part of dragon, 0.2 part of cordate houttuynia, 0.2 part of the achene of Siberian cocklebur, 0.2 part of wild aconite root, 0.2 part of sweet wormwood, 0.2 part of asafoetide.
The preparation method of the feed of described treatment testudinate fatty liver disease, comprises the following steps:
S1: respectively silkworm chrysalis, cassava are dried and pulverized at 45 DEG C, excessively mix after 150 object sieves, make mixtureA;
S2: by Sophora alopecuroide, mistletoe, the bark of ash, Radix Isatidis, wild marjoram, plantain seed, bitter dish, herba aristolochiae, the Radix Astragali, field pennycress,The root of Dahurain angelica, the root of kudzu vine, dandelion, Chinese lobelia, Herba Siegesbeckiae, honeysuckle, the bark of eucommia, radix bardanae, lily, earthworm, cordate houttuynia, greyThe ears or side handles of a utensil, wild aconite root, sweet wormwood, asafoetide co-grinding, obtain mixture B, mixture B joined in ethanol, at ultrasonic waveAssisted Extraction is followed the example of lower extraction liquid, and condition is: ultrasonic power is 150W, and concentration of alcohol is 50%, and extracting temperature is 50 DEG C,Extraction time is 50min; The medical filtration of gained, is concentrated into water content 10%, obtains mixture C; The mixture C of gained is at 95 DEG CUnder be dried to water content 4%, pulverize, 120 mesh sieve that sieve, powder is 135 DEG C in temperature, pressure is under 220KPa, sterilizing2min, makes Chinese medicine preparation;
S3: the mixture A that step S1 is made, the Chinese medicine preparation that step S2 makes, salt, dimethyl-β-DMPT,Betaine, glycine, cytidylic acid, holder Martin polypeptide, citronellal, and 300 parts of water, mix again, systemObtain feed crude product;
S4: the feed crude product that step S3 is made make diameter be the particle of 0.05-0.2cm and temperature be at 90 DEG C dry extremelyAfter water content is 6%, be 135 DEG C in temperature, pressure is under 220KPa, and sterilizing 3min makes treatment testudinate fatty liver diseaseFeed.
Embodiment 2
Treat a feed for testudinate fatty liver disease, taking weight portion as unit, comprise following raw material: 50 parts of dried silkworm chrysalis meals, cassava70 parts, powder, 16 parts of salt, 14 parts of dimethyl-β-DMPTs, 9 parts of betaines, 8 parts of glycine, cytidylic acid8 parts, 0.6 part of holder Martin polypeptide, 3 parts of citronellals, 42 parts of Chinese medicine preparations, described Chinese medicine preparation comprises the former of following weight portionMaterial: 3 parts of Sophora alopecuroides, 3 parts of mistletoes, 3 parts of the barks of ash, 3 parts of Radix Isatidis, 3 parts of wild marjorams, 2 parts of plantain seeds, 2 parts of bitter dishes,2 parts of herba aristolochiaes, 2 parts of the Radixs Astragali, 2 parts of field pennycress, 1.6 parts of the roots of Dahurain angelica, 1.6 parts of the roots of kudzu vine, 1.6 parts of dandelions, Chinese lobelia 1.6Part, 1.6 parts of Herba Siegesbeckiaes, 1.2 parts of honeysuckles, 1.2 parts of the barks of eucommia, 1.2 parts of radix bardanaes, 1.2 parts of lilies, 1.2 parts of earthworms,0.6 part of cordate houttuynia, 0.6 part of the achene of Siberian cocklebur, 0.6 part of wild aconite root, 0.6 part of sweet wormwood, 0.6 part of asafoetide.
The preparation method of the feed of described treatment testudinate fatty liver disease, comprises the following steps:
S1: respectively silkworm chrysalis, cassava are dried and pulverized at 60 DEG C, excessively mix after 250 object sieves, make mixtureA;
S2: by Sophora alopecuroide, mistletoe, the bark of ash, Radix Isatidis, wild marjoram, plantain seed, bitter dish, herba aristolochiae, the Radix Astragali, field pennycress,The root of Dahurain angelica, the root of kudzu vine, dandelion, Chinese lobelia, Herba Siegesbeckiae, honeysuckle, the bark of eucommia, radix bardanae, lily, earthworm, cordate houttuynia, greyThe ears or side handles of a utensil, wild aconite root, sweet wormwood, asafoetide co-grinding, obtain mixture B, mixture B joined in butanone, at ultrasonic waveAssisted Extraction is followed the example of lower extraction liquid, and condition is: ultrasonic power is 170W, and butanone concentration is 80%, and extracting temperature is 70 DEG C,Extraction time is 30min; The medical filtration of gained, is concentrated into water content 22%, obtains mixture C; The mixture C of gained is at 105 DEG CUnder be dried to water content 6%, pulverize, 320 mesh sieve that sieve, powder is 136 DEG C in temperature, pressure is under 230KPa, sterilizing1min, makes Chinese medicine preparation;
S3: the mixture A that step S1 is made, the Chinese medicine preparation that step S2 makes, salt, dimethyl-β-DMPT,Betaine, glycine, cytidylic acid, holder Martin polypeptide, citronellal, and 700 parts of water, mix again, systemObtain feed crude product;
S4: the feed crude product that step S3 is made make diameter be the particle of 0.05-0.2cm and temperature be at 100 DEG C dryAfter being 4% to water content, be 136 DEG C in temperature, pressure is under 230KPa, and sterilizing 3min makes treatment testudinate fatty liverSick feed.
Embodiment 3
Treat a feed for testudinate fatty liver disease, taking weight portion as unit, comprise following raw material: 40 parts of dried silkworm chrysalis meals, cassava60 parts, powder, 12 parts of salt, 12 parts of dimethyl-β-DMPTs, 8 parts of betaines, 6 parts of glycine, cytidylic acid6 parts, 0.4 part of holder Martin polypeptide, 2 parts of citronellals, 28.5 parts of Chinese medicine preparations, described Chinese medicine preparation comprises following weight portionRaw material: 2 parts of Sophora alopecuroides, 2 parts of mistletoes, 2 parts of the barks of ash, 2 parts of Radix Isatidis, 2 parts of wild marjorams, 1.5 parts of plantain seeds, bitter dish1.5 parts, 1.5 parts of herba aristolochiaes, 1.5 parts of the Radixs Astragali, 1.5 parts of field pennycress, 1 part of the root of Dahurain angelica, 1 part of the root of kudzu vine, 1 part of dandelion,1 part of Chinese lobelia, 1 part of Herba Siegesbeckiae, 0.8 part of honeysuckle, 0.8 part of the bark of eucommia, 0.8 part of radix bardanae, 0.8 part of lily, earthworm0.8 part, 0.4 part of cordate houttuynia, 0.4 part of the achene of Siberian cocklebur, 0.4 part of wild aconite root, 0.4 part of sweet wormwood, 0.4 part of asafoetide.
The preparation method of the feed of described treatment testudinate fatty liver disease, comprises the following steps:
S1: respectively silkworm chrysalis, cassava are dried and pulverized at 55 DEG C, excessively mix after 200 object sieves, make mixtureA;
S2: by Sophora alopecuroide, mistletoe, the bark of ash, Radix Isatidis, wild marjoram, plantain seed, bitter dish, herba aristolochiae, the Radix Astragali, field pennycress,The root of Dahurain angelica, the root of kudzu vine, dandelion, Chinese lobelia, Herba Siegesbeckiae, honeysuckle, the bark of eucommia, radix bardanae, lily, earthworm, cordate houttuynia, greyThe ears or side handles of a utensil, wild aconite root, sweet wormwood, asafoetide co-grinding, obtain mixture B, mixture B joined in trichloroethanes, superSound wave assisted Extraction is followed the example of lower extraction liquid, and condition is: ultrasonic power is 160W, and trichloroethanes concentration is 65%, extracts temperatureBe 60 DEG C, extraction time is 40min; The medical filtration of gained, is concentrated into water content 15%, obtains mixture C; The mixing of gainedThing C is dried to water content 5% at 100 DEG C, pulverizes, and 200 mesh sieve that sieve, powder is 136 DEG C in temperature, pressure is 230KPaUnder, sterilizing 1min, makes Chinese medicine preparation;
S3: the mixture A that step S1 is made, the Chinese medicine preparation that step S2 makes, salt, dimethyl-β-DMPT,Betaine, glycine, cytidylic acid, holder Martin polypeptide, citronellal, and 500 parts of water, mix again, systemObtain feed crude product;
S4: the feed crude product that step S3 is made make diameter be the particle of 0.05-0.2cm and temperature be at 95 DEG C dry extremelyAfter water content is 5%, be 136 DEG C in temperature, pressure is under 230KPa, and sterilizing 2min makes treatment testudinate fatty liver diseaseFeed.
Embodiment 4
Treat a feed for testudinate fatty liver disease, taking weight portion as unit, comprise following raw material: 35 parts of dried silkworm chrysalis meals, cassava55 parts, powder, 10 parts of salt, 8 parts of dimethyl-β-DMPTs, 6 parts of betaines, 5 parts of glycine, cytidylic acid5 parts, 0.3 part of holder Martin polypeptide, 1.5 parts of citronellals, 21 parts of Chinese medicine preparations, described Chinese medicine preparation comprises following weight portionRaw material: 1.5 parts of Sophora alopecuroides, 1.5 parts of mistletoes, 1.5 parts of the barks of ash, 1.5 parts of Radix Isatidis, 1.5 parts of wild marjorams, plantain seed 1Part, 1 part of bitter dish, 1 part of herba aristolochiae, 1 part of the Radix Astragali, 1 part of field pennycress, 0.8 part of the root of Dahurain angelica, 0.8 part of the root of kudzu vine, dandelion 0.8Part, 0.8 part of Chinese lobelia, 0.8 part of Herba Siegesbeckiae, 0.6 part of honeysuckle, 0.6 part of the bark of eucommia, 0.6 part of radix bardanae, lily 0.6Part, 0.6 part of earthworm, 0.3 part of cordate houttuynia, 0.3 part of the achene of Siberian cocklebur, 0.3 part of wild aconite root, 0.3 part of sweet wormwood, 0.3 part of asafoetide.
The preparation method of the feed of described treatment testudinate fatty liver disease, comprises the following steps:
S1: respectively silkworm chrysalis, cassava are dried and pulverized at 50 DEG C, excessively mix after 250 object sieves, make mixtureA;
S2: by Sophora alopecuroide, mistletoe, the bark of ash, Radix Isatidis, wild marjoram, plantain seed, bitter dish, herba aristolochiae, the Radix Astragali, field pennycress,The root of Dahurain angelica, the root of kudzu vine, dandelion, Chinese lobelia, Herba Siegesbeckiae, honeysuckle, the bark of eucommia, radix bardanae, lily, earthworm, cordate houttuynia, greyThe ears or side handles of a utensil, wild aconite root, sweet wormwood, asafoetide co-grinding, obtain mixture B, mixture B joined in carbon disulfide, superSound wave assisted Extraction is followed the example of lower extraction liquid, and condition is: ultrasonic power is 155W, and Carbon Disulfide is 55%, extracts temperatureBe 55 DEG C, extraction time is 45min; The medical filtration of gained, is concentrated into water content 14%, obtains mixture C; The mixing of gainedThing C is dried to water content 6% at 95 DEG C, pulverizes, and 150 mesh sieve that sieve, powder is 135 DEG C in temperature, pressure is 220KPaUnder, sterilizing 2min, makes Chinese medicine preparation;
S3: the mixture A that step S1 is made, the Chinese medicine preparation that step S2 makes, salt, dimethyl-β-DMPT,Betaine, glycine, cytidylic acid, holder Martin polypeptide, citronellal, and 400 parts of water, mix again, systemObtain feed crude product;
S4: the feed crude product that step S3 is made make diameter be the particle of 0.05-0.2cm and temperature be at 100 DEG C dryAfter being 6% to water content, be 135 DEG C in temperature, pressure is under 220KPa, and sterilizing 3min makes treatment testudinate fatty liverSick feed.
Embodiment 5
Treat a feed for testudinate fatty liver disease, taking weight portion as unit, comprise following raw material: 45 parts of dried silkworm chrysalis meals, cassava65 parts, powder, 14 parts of salt, 12 parts of dimethyl-β-DMPTs, 8 parts of betaines, 7 parts of glycine, cytidylic acid7 parts, 0.5 part of holder Martin polypeptide, 2.5 parts of citronellals, 36 parts of Chinese medicine preparations, described Chinese medicine preparation comprises following weight portionRaw material: 2.5 parts of Sophora alopecuroides, 2.5 parts of mistletoes, 2.5 parts of the barks of ash, 2.5 parts of Radix Isatidis, 2.5 parts of wild marjorams, plantain seed 1.8Part, 1.8 parts of bitter dishes, 1.8 parts of herba aristolochiaes, 1.8 parts of the Radixs Astragali, 1.8 parts of field pennycress, 1.4 parts of the roots of Dahurain angelica, 1.4 parts of the roots of kudzu vine,1.4 parts of dandelions, 1.4 parts of Chinese lobelia, 1.4 parts of Herba Siegesbeckiaes, 1 part of honeysuckle, 1 part of the bark of eucommia, 1 part of radix bardanae, lily1 part, 1 part of earthworm, 0.5 part of cordate houttuynia, 0.5 part of the achene of Siberian cocklebur, 0.5 part of wild aconite root, 0.5 part of sweet wormwood, 0.5 part of asafoetide.
The preparation method of the feed of described treatment testudinate fatty liver disease, comprises the following steps:
S1: respectively silkworm chrysalis, cassava are dried and pulverized at 50 DEG C, excessively mix after 200 object sieves, make mixtureA;
S2: by Sophora alopecuroide, mistletoe, the bark of ash, Radix Isatidis, wild marjoram, plantain seed, bitter dish, herba aristolochiae, the Radix Astragali, field pennycress,The root of Dahurain angelica, the root of kudzu vine, dandelion, Chinese lobelia, Herba Siegesbeckiae, honeysuckle, the bark of eucommia, radix bardanae, lily, earthworm, cordate houttuynia, greyThe ears or side handles of a utensil, wild aconite root, sweet wormwood, asafoetide co-grinding, obtain mixture B, and mixture B is joined to trichloroethanes and curingIn carbon mixed liquor, follow the example of lower extraction liquid at ultrasonic wave assisted Extraction, condition is: ultrasonic power is 165W, trichloroethanes and twoNitric sulfid mixed liquid concentration is 75%, and extracting temperature is 65 DEG C, and extraction time is 35min; The medical filtration of gained, is concentrated intoWater content 20%, obtains mixture C; The mixture C of gained is dried to water content 4% at 100 DEG C, pulverizes, and 250 mesh sieves sieveSon, powder is 136 DEG C in temperature, and pressure is under 230KPa, and sterilizing 1min, makes Chinese medicine preparation;
S3: the mixture A that step S1 is made, the Chinese medicine preparation that step S2 makes, salt, dimethyl-β-DMPT,Betaine, glycine, cytidylic acid, holder Martin polypeptide, citronellal, and 600 parts of water, mix again, systemObtain feed crude product;
S4: the feed crude product that step S3 is made make diameter be the particle of 0.05-0.2cm and temperature be at 100 DEG C dryAfter being 6% to water content, be 136 DEG C in temperature, pressure is under 230KPa, and sterilizing 2min makes treatment testudinate fatty liverSick feed.
For the effect of checking feed of the present invention, by following experimental verification.
Experiment one
Select almost 540 of small water turtles consistent and that make a definite diagnosis fatty liver disease of 0.21-0.32kg, specification, be divided at random three groups,Be control group, test group 1, test group 2, test group 3, test group 4, test group 5, every group is done 3 parallel laboratory tests, every30 of group small water turtles. Control group small water turtle feed with dried silkworm chrysalis meal, tapioca starch, salt, dimethyl-β-DMPT, betaine,The basic feed that glycine, cytidylic acid, holder Martin polypeptide, citronellal are mixed with; The small water turtle of test group 1 is fed thisThe feed of inventive embodiments 1; The feed feed of the embodiment of the present invention 2 of the small water turtle of test group 2; The small water turtle of test group 3 is raisedFeed the feed of the embodiment of the present invention 3; The feed feed of the embodiment of the present invention 4 of the small water turtle of test group 4; The money of test group 5The feed feed of the embodiment of the present invention 5 of tortoise. Feed continuously 10 days, feed intake every day 2 times, experimental period 10 days, finishes rear meterCalculate the average daily gain amount of small water turtle, average day food ration, feed coefficient, the death rate and cure rate, the results are shown in following table.
As seen from the above table, test group and control group contrast, average daily gain amount 0.807-0.888g, average day food ration increases0.336-0.351g, feed coefficient reduces 0.275-0.3, and the death rate reduces 31.12%-32.23%, and cure rate improves50%-52.22%, illustrates that feed of the present invention can improve feed intake and the speed of growth of suffering from fatty liver disease small water turtle, strengthens goldThe immunity of money tortoise and anti-stress ability, reduce the death rate that small water turtle is suffered from fatty liver disease, improves cure rate.
Experiment two
Select almost 540 of yellow sand soft-shelled turtles consistent and that make a definite diagnosis fatty liver disease of 0.22-0.31kg, specification, be divided at random three groups,Be control group, test group 1, test group 2, test group 3, test group 4, test group 5, every group is done 3 parallel laboratory tests, every30 of group yellow sand soft-shelled turtles. Control group yellow sand soft-shelled turtle feed with dried silkworm chrysalis meal, tapioca starch, salt, dimethyl-β-DMPT, betaine,The basic feed that glycine, cytidylic acid, holder Martin polypeptide, citronellal are mixed with; The yellow sand soft-shelled turtle of test group 1 is fed thisThe feed of inventive embodiments 1; The feed feed of the embodiment of the present invention 2 of the yellow sand soft-shelled turtle of test group 2; The yellow sand soft-shelled turtle of test group 3 is raisedFeed the feed of the embodiment of the present invention 3; The feed feed of the embodiment of the present invention 4 of the yellow sand soft-shelled turtle of test group 4; The yellow sand of test group 5The feed feed of the embodiment of the present invention 5 of soft-shelled turtle. Feed continuously 10 days, feed intake every day 2 times, experimental period 10 days, finishes rear meterCalculate the average daily gain amount of yellow sand soft-shelled turtle, average day food ration, feed coefficient, the death rate and cure rate, the results are shown in following table.
As seen from the above table, test group and control group contrast, average daily gain amount 0.802-0.884g, average day food ration increases0.33-0.348g, feed coefficient reduces 0.274-0.298, and the death rate reduces 32.22%-34.45%, and cure rate improves50%-51.11%, illustrates that feed of the present invention can improve feed intake and the speed of growth of suffering from fatty liver disease yellow sand soft-shelled turtle, strengthens yellowImmunity and the anti-stress ability of husky soft-shelled turtle, reduce the death rate that yellow sand soft-shelled turtle suffers from fatty liver disease, improves cure rate.
Above content can not assert that the present invention is concrete and implement to be confined to these explanations, common for the technical field of the inventionTechnical staff, conceives under prerequisite not departing from the present invention, can also make some simple deduction or replace, all should be considered asBelong to the present invention by the definite scope of patent protection of submitted to claims.

Claims (10)

1. a feed for the treatment of testudinate fatty liver disease, is characterized in that, taking weight portion as unit, comprises following raw material: dried silkworm chrysalis meal 30-50 part, tapioca starch 50-70 part, salt 8-16 part, dimethyl-β-DMPT 6-14 part, betaine 5-9 part, glycine 4-8 part, cytidylic acid 4-8 part, holder Martin polypeptide 0.2-0.6 part, citronellal 1-3 part, Chinese medicine preparation 15-42 part, described Chinese medicine preparation comprises the raw material of following weight portion: Sophora alopecuroide 1-3 part, mistletoe 1-3 part, bark of ash 1-3 part, Radix Isatidis 1-3 part, wild marjoram 1-3 part, plantain seed 0.8-2 part, bitter dish 0.8-2 part, herba aristolochiae 0.8-2 part, Radix Astragali 0.8-2 part, field pennycress 0.8-2 part, root of Dahurain angelica 0.6-1.6 part, root of kudzu vine 0.6-1.6 part, dandelion 0.6-1.6 part, Chinese lobelia 0.6-1.6 part, Herba Siegesbeckiae 0.6-1.6 part, honeysuckle 0.4-1.2 part, bark of eucommia 0.4-1.2 part, radix bardanae 0.4-1.2 part, lily 0.4-1.2 part, earthworm 0.4-1.2 part, cordate houttuynia 0.2-0.6 part, achene of Siberian cocklebur 0.2-0.6 part, wild aconite root 0.2-0.6 part, sweet wormwood 0.2-0.6 part, asafoetide 0.2-0.6 part.
2. the feed for the treatment of testudinate fatty liver disease according to claim 1, is characterized in that, taking weight portion as unit, comprises following raw material: dried silkworm chrysalis meal 35-45 part, tapioca starch 55-65 part, salt 10-14 part, dimethyl-β-DMPT 8-12 part, betaine 6-8 part, glycine 5-7 part, cytidylic acid 5-7 part, holder Martin polypeptide 0.3-0.5 part, citronellal 1.5-2.5 part, Chinese medicine preparation 21-36 part, described Chinese medicine preparation comprises the raw material of following weight portion: Sophora alopecuroide 1.5-2.5 part, mistletoe 1.5-2.5 part, bark of ash 1.5-2.5 part, Radix Isatidis 1.5-2.5 part, wild marjoram 1.5-2.5 part, plantain seed 1-1.8 part, bitter dish 1-1.8 part, herba aristolochiae 1-1.8 part, Radix Astragali 1-1.8 part, field pennycress 1-1.8 part, root of Dahurain angelica 0.8-1.4 part, root of kudzu vine 0.8-1.4 part, dandelion 0.8-1.4 part, Chinese lobelia 0.8-1.4 part, Herba Siegesbeckiae 0.8-1.4 part, honeysuckle 0.6-1 part, bark of eucommia 0.6-1 part, radix bardanae 0.6-1 part, lily 0.6-1 part, earthworm 0.6-1 part, cordate houttuynia 0.3-0.5 part, achene of Siberian cocklebur 0.3-0.5 part, wild aconite root 0.3-0.5 part, sweet wormwood 0.3-0.5 part, asafoetide 0.3-0.5 part.
3. the feed for the treatment of testudinate fatty liver disease according to claim 2, it is characterized in that, taking weight portion as unit, comprise following raw material: 40 parts of dried silkworm chrysalis meals, 60 parts of tapioca starch, 12 parts of salt, 12 parts of dimethyl-β-DMPTs, 8 parts of betaines, 6 parts of glycine, 6 parts of cytidylic acids, 0.4 part of holder Martin polypeptide, 2 parts of citronellals, 28.5 parts of Chinese medicine preparations, described Chinese medicine preparation comprises the raw material of following weight portion: 2 parts of Sophora alopecuroides, 2 parts of mistletoes, 2 parts of the barks of ash, 2 parts of Radix Isatidis, 2 parts of wild marjorams, 1.5 parts of plantain seeds, 1.5 parts of bitter dishes, 1.5 parts of herba aristolochiaes, 1.5 parts of the Radixs Astragali, 1.5 parts of field pennycress, 1 part of the root of Dahurain angelica, 1 part of the root of kudzu vine, 1 part of dandelion, 1 part of Chinese lobelia, 1 part of Herba Siegesbeckiae, 0.8 part of honeysuckle, 0.8 part of the bark of eucommia, 0.8 part of radix bardanae, 0.8 part of lily, 0.8 part of earthworm, 0.4 part of cordate houttuynia, 0.4 part of the achene of Siberian cocklebur, 0.4 part of wild aconite root, 0.4 part of sweet wormwood, 0.4 part of asafoetide.
4. according to a preparation method for the feed of the treatment testudinate fatty liver disease described in claim 1-3 any one, it is characterized in that, comprise the following steps:
S1: respectively silkworm chrysalis, cassava are dried and pulverized at 45-60 DEG C, excessively mix after 150-250 object sieve, make mixture A;
S2: by Sophora alopecuroide, mistletoe, the bark of ash, Radix Isatidis, wild marjoram, plantain seed, bitter dish, herba aristolochiae, the Radix Astragali, field pennycress, the root of Dahurain angelica, the root of kudzu vine, dandelion, Chinese lobelia, Herba Siegesbeckiae, honeysuckle, the bark of eucommia, radix bardanae, lily, earthworm, cordate houttuynia, the achene of Siberian cocklebur, wild aconite root, sweet wormwood, asafoetide co-grinding, obtain mixture B, mixture B is joined in organic solvent, follow the example of lower extraction liquid at ultrasonic wave assisted Extraction, the medical filtration of gained, concentrated, obtain mixture C, the mixture C of gained is dried, pulverizes, sieves, sterilizing, makes Chinese medicine preparation;
S3: the mixture A that step S1 is made, the Chinese medicine preparation that step S2 makes, salt, dimethyl-β-DMPT, betaine, glycine, cytidylic acid, holder Martin polypeptide, citronellal, and 300-700 part water, again mix, make feed crude product;
S4: the feed crude product that step S3 is made is made diameter to be the particle of 0.05-0.2cm and to be to dry to water content≤6% at 90-100 DEG C in temperature, be 135-136 DEG C in temperature, pressure is under 220-230KPa, and sterilizing 2-3min makes the feed for the treatment of testudinate fatty liver disease.
5. the preparation method of the feed for the treatment of testudinate fatty liver disease according to claim 4, is characterized in that, organic solvent described in step S2 comprises one or more in ethanol, butanone, trichloroethanes, carbon disulfide.
6. the preparation method of the feed for the treatment of testudinate fatty liver disease according to claim 4, it is characterized in that, the condition that the assisted Extraction of ultrasonic wave described in step S2 is followed the example of extraction liquid is: ultrasonic power is 150-170W, organic solvent concentration is 50%-80%, extraction temperature is 50-70 DEG C, and extraction time is 30-50min.
7. the preparation method of the feed for the treatment of testudinate fatty liver disease according to claim 4, is characterized in that, step S2 herb liquid filters, and is concentrated into water content 10%-22%.
8. the preparation method of the feed for the treatment of testudinate fatty liver disease according to claim 4, is characterized in that, in step S2, mixture B is dried to water content 4%-6% at 95-105 DEG C.
9. the preparation method of the feed for the treatment of testudinate fatty liver disease according to claim 4, is characterized in that, the condition of sieving in step S2 is: cross 120-320 mesh sieve.
10. the preparation method of the feed for the treatment of testudinate fatty liver disease according to claim 4, is characterized in that, in step S2, the condition of sterilizing is: temperature is 135-136 DEG C, and pressure is 220-230KPa, and the time is 1-2min.
CN201510960315.5A 2015-12-21 2015-12-21 Feed for treating fatty liver disease of turtles and preparation method thereof Pending CN105581031A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107348117A (en) * 2017-08-14 2017-11-17 马鞍山中粮生物化学有限公司 A kind of breeding soft-shell turtle feed and its preparation technology
CN107439845A (en) * 2017-08-15 2017-12-08 兰溪市酉泽饲料技术服务有限公司 Add the preparation method of the black carp health-care feed of Chinese herbal medicine

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CN104381645A (en) * 2014-11-04 2015-03-04 界首市张珉土元养殖专业合作社 Eupolyphaga feed with good palatability and high nutrient content
CN104799048A (en) * 2015-01-15 2015-07-29 南宁市春华农业科技有限公司 Feed capable of improving Truogx sinensis disease resistance, and preparation method thereof
CN104905049A (en) * 2015-05-27 2015-09-16 石东秀 Feed for treating Cyclemys trifasciata bacterial enterorrhagia, and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104381645A (en) * 2014-11-04 2015-03-04 界首市张珉土元养殖专业合作社 Eupolyphaga feed with good palatability and high nutrient content
CN104799048A (en) * 2015-01-15 2015-07-29 南宁市春华农业科技有限公司 Feed capable of improving Truogx sinensis disease resistance, and preparation method thereof
CN104905049A (en) * 2015-05-27 2015-09-16 石东秀 Feed for treating Cyclemys trifasciata bacterial enterorrhagia, and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107348117A (en) * 2017-08-14 2017-11-17 马鞍山中粮生物化学有限公司 A kind of breeding soft-shell turtle feed and its preparation technology
CN107439845A (en) * 2017-08-15 2017-12-08 兰溪市酉泽饲料技术服务有限公司 Add the preparation method of the black carp health-care feed of Chinese herbal medicine

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