CN105567745A - Method for improving utilization of xylose in lignocellulose hydrolysate - Google Patents
Method for improving utilization of xylose in lignocellulose hydrolysate Download PDFInfo
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- CN105567745A CN105567745A CN201610046770.9A CN201610046770A CN105567745A CN 105567745 A CN105567745 A CN 105567745A CN 201610046770 A CN201610046770 A CN 201610046770A CN 105567745 A CN105567745 A CN 105567745A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention discloses a method for improving utilization of xylose in lignocellulose hydrolysate. The method includes the following steps of 1, raw material pretreatment, wherein pretreated raw materials are divided into the part A and the part B; 2, pretreated raw material washing, wherein the part A of the pretreated raw materials is taken, water is added, pH is adjusted, separating is carried out, and liquid and solid are obtained; 3, liquid fermentation, the pH of the liquid is adjusted, and recombined saccharomyces cerevisiae is inoculated and cultured; 4, enzymolysis, wherein the part B of the pretreated raw materials is added into the solid obtained in the step 2, mixing is carried out, pH is adjusted, cellulose and glucosidase are added, and enzymolysis is carried out; 5, mixing and fermentation, wherein the fermentation liquid and the enzymolysis liquid are merged and mixed to be even, pH is adjusted, culturing is carried out, and the fermentation liquid rich in ethyl alcohol is obtained. By means of the method, inhibition of an inhibitor in the pretreated lignocellulose hydrolysate to recombined saccharomyces is effectively reduced, the tolerance of the recombined saccharomyces and the utilization of the xylose in the hydrolysate are improved, the use rate of the xylose is increased, and the yield of ethyl alcohol is increased.
Description
Technical field
The present invention relates to cellulosic ethanol technical field, relate to a kind of method improving xylose utilization in ligno-cellulose hydrolysate particularly.
Background technology
Conveniently transport because of liquid fuel and be convenient to utilize, becoming important industry and daily energy derive, oil is that at present the whole world uses maximum liquid fuels, but, the non-renewable and CO of petroleum resources
2the deterioration of environment can be caused with the discharge of other dusty gass.Develop the most important thing that reproducible clean energy becomes the energy development strategy of China.Ethanol is paid close attention to widely as the alternative cleaning liq energy and is paid attention to, the method of traditional mode of production ethanol take grain as raw material, adopt the method for yeast direct fermentation to produce ethanol, but this kind of method needs to consume a large amount of grain, face the problem of " strive ground with people, strive grain with people ".People turn one's attention to agricultural wastes gradually, as maize straw, broomcorn straw, wheat stalks etc., because agricultural wastes are also carbohydrate, and annual output is huge, maize straw, broomcorn straw, wheat stalk etc. just have 700,000,000 tons annual, these wastes can not get effective utilization and just can be burnt by peasant, not only waste energy but also contaminate environment.The glucose and xylose utilizing agricultural wastes and lignocellulose to obtain through enzymolysis after pre-treatment can utilize by yeast generation cellulosic ethanol; adopt and namely protect environment in this way; effectively make use of resource; having got back clean energy, is very promising Energy utilization patterns.
Lignocellulose is primarily of Mierocrystalline cellulose, hemicellulose, xylogen composition, and in agricultural wastes, the content of three is approximately: 30% ~ 40% Mierocrystalline cellulose, 20% ~ 30% hemicellulose, 10% ~ 25% xylogen.Wherein, glucose is cellulosic component units, and wood sugar is the main component units of hemicellulose.In sponge hydrolyzed solution, wood sugar accounts for about 30%, is the sugar being only second to glucose, and therefore, the common utilization of glucose and xylose is one of gordian technique of exploitation lignocellulose ethanol.
At present, although the yeast saccharomyces cerevisiae transformed by gene engineering can utilize wood sugar, because metabolism own is not inclined to wood sugar, the utilization of wood sugar is easily subject to the suppression of by product in hydrolyzed solution.Improve zymotechnique, improve the utilization of wood sugar in hydrolyzed solution, be improve output, reduce the effective way of the wasting of resources.
Summary of the invention
Utilize wood sugar to be subject to inhibitor impact for recombination yeast, the invention provides a kind of method improving xylose utilization in ligno-cellulose hydrolysate.
Technical scheme of the present invention is summarized as follows:
Improve the method for xylose utilization in ligno-cellulose hydrolysate, comprise the steps:
(1) raw materials pretreatment: working concentration is that the aqueous acid of 5g/L ~ 50g/L soaks lignocellulosic material 10 ~ 24h, passes into water vapor, after making temperature rise to 180 ~ 220 DEG C, maintains 3 ~ 15 minutes, venting, cooling; Obtain pretreating raw material; The weight ratio of described lignocellulosic material dry weight and aqueous acid is 1:0.5 ~ 2; Pretreating raw material is divided into A, B two portions by weight the ratio for 1:0.2-1;
(2) water-washing pre-treatment raw material: get pretreating raw material part A and add water, makes the dry matter content of pretreating raw material part A be 10% ~ 20%, regulates pH to 4.0 ~ 6.0, and mixing is separated, obtains liquid and solid;
(3) liquid fermenting: pH to 5.0 ~ 7.0 of the liquid that regulating step (2) obtains, access deposit number is the recombinant Saccharomyces cerevisiae (Saccharomycescerevisiae) of CGMCCNo.6634; Inoculum size is 1 ~ 15g/L; 26 ~ 37 DEG C, under rotating speed is the condition of 100 ~ 400rpm, or at 26 ~ 37 DEG C, air flow is under the condition of 0.005vvm ~ 0.08vvm, cultivates 12-48h; ;
(4) enzymolysis: add pretreating raw material part B in the solid that step (2) obtains, mixing, regulate pH to 4.0 ~ 6.0, add cellulase and glucuroide, cellulase is made to be 10 ~ 30FPU/g, glucuroide is made to be 20 ~ 50CBU/g, at 30 ~ 60 DEG C of enzymolysis 12 ~ 48h;
(5) mixed fermentation: the enzymolysis solution that the fermented liquid obtain step (3) and step (4) obtain merges, mixing, regulates pH to 5.0 ~ 7.0; Temperature 26 ~ 37 DEG C, rotating speed is under the condition of 100 ~ 400rpm, or at 26 ~ 37 DEG C, air flow is under the condition of 0.005vvm ~ 0.08vvm, cultivates 60 ~ 120h, obtains the fermented liquid being rich in ethanol.
Step (1) described acid is sulfuric acid, hydrochloric acid, oxalic acid or formic acid.
Lignocellulose is maize straw, broomcorn straw, bagasse, at least one in corn cob and wheat stalk.
The alkali regulating pH can be the material such as ammoniacal liquor, NaOH, KOH.
Advantage of the present invention:
Method of the present invention to effectively reduce in pretreated ligno-cellulose hydrolysate inhibitor to the restraining effect of recombination yeast, improves the utilization of wood sugar in the tolerance of recombination yeast and hydrolyzed solution.Improve xylose utilization rate, strengthen alcohol yied.
Embodiment
Below by specific embodiment, the present invention is further illustrated.
The yeast saccharomyces cerevisiae called after SyBE005 that the present invention uses, be deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) on September 27th, 2012, its deposit number is CGMCCNo6634, and the classification of suggestion is called: Saccharomyces Cerevisiae in S accharomycescerevisiae.And survive.Preservation address is Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101, phone 010-64807355.
Embodiment 1
Improve the method for xylose utilization in ligno-cellulose hydrolysate, comprise the steps:
(1) raw materials pretreatment: working concentration is that 25g/L aqueous hydrochloric acid soaks lignocellulose (maize straw) raw material 17h, passes into water vapor, after making temperature rise to 200 DEG C, maintains 9 minutes, venting, cooling; Obtain pretreating raw material; The weight ratio of described lignocellulosic material dry weight and aqueous acid is 1:1; Pretreating raw material is divided into A, B two portions by weight the ratio for 1:0.5;
(2) water-washing pre-treatment raw material: get pretreating raw material part A and add water, makes the dry matter content of pretreating raw material part A be 15%, regulates pH to 5.0 with the KOH aqueous solution, and mixing is separated, obtains liquid and solid;
(3) liquid fermenting: the pH to 6.0 of the liquid that regulating step (2) obtains, access deposit number is the recombinant Saccharomyces cerevisiae (Saccharomycescerevisiae) of CGMCCNo.6634; Inoculum size is 10g/L; 30 DEG C, under rotating speed is the condition of 200rpm, cultivate 30h;
(4) enzymolysis: add pretreating raw material part B in the solid that step (2) obtains, mixing, regulates pH to 5.0, add cellulase and glucuroide, make cellulase be 20FPU/g, make glucuroide be 35CBU/g, at 45 DEG C of enzymolysis 36h;
(5) mixed fermentation: the enzymolysis solution that the fermented liquid obtain step (3) and step (4) obtain merges, mixing, regulates pH to 6.0; Temperature 30 DEG C, rotating speed is under the condition of 200rpm, cultivates 90h, obtains the fermented liquid being rich in ethanol.Alcohol yied is 65.1%.
Embodiment 2
Improve the method for xylose utilization in ligno-cellulose hydrolysate, comprise the steps:
(1) raw materials pretreatment: working concentration is that the aqueous sulfuric acid of 5g/L soaks lignocellulose (broomcorn straw) raw material 24h, passes into water vapor, after making temperature rise to 180 DEG C, maintains 15 minutes, venting, cooling; Obtain pretreating raw material; The weight ratio of described lignocellulosic material dry weight and aqueous acid is 1:0.5; Pretreating raw material is divided into A, B two portions by weight the ratio for 1:0.2;
(2) water-washing pre-treatment raw material: get pretreating raw material part A and add water, makes the dry matter content of pretreating raw material part A be 10%, regulates pH to 4.0 with the NaOH aqueous solution, and mixing is separated, obtains liquid and solid;
(3) liquid fermenting: the pH to 5.0 of the liquid that regulating step (2) obtains, access deposit number is the recombinant Saccharomyces cerevisiae (Saccharomycescerevisiae) of CGMCCNo.6634; Inoculum size is 1g/L; 37 DEG C, under rotating speed is the condition of 100rpm, cultivate 48h;
(4) enzymolysis: add pretreating raw material part B in the solid that step (2) obtains, mixing, regulates pH to 4.0, add cellulase and glucuroide, make cellulase be 10FPU/g, make glucuroide be 50CBU/g, at 30 DEG C of enzymolysis 48h;
(5) mixed fermentation: the enzymolysis solution that the fermented liquid obtain step (3) and step (4) obtain merges, mixing, regulates pH to 5.0; Temperature 26 DEG C, rotating speed is under the condition of 100rpm, cultivates 60h, obtains the fermented liquid being rich in ethanol.Alcohol yied is 58.7%.
Embodiment 3
Improve the method for xylose utilization in ligno-cellulose hydrolysate, comprise the steps:
(1) raw materials pretreatment: working concentration is that the oxalic acid aqueous solution of 50g/L soaks lignocellulosic material (weight ratio is the bagasse of 1:1:1, corn cob and wheat stalk) 10h, passes into water vapor, after making temperature rise to 220 DEG C, maintain 3 minutes, venting, cooling; Obtain pretreating raw material; The weight ratio of described lignocellulosic material dry weight and aqueous acid is 1:2; Pretreating raw material is divided into A, B two portions by weight the ratio for 1:1;
(2) water-washing pre-treatment raw material: get pretreating raw material part A and add water, makes the dry matter content of pretreating raw material part A be 20%, regulates pH to 6.0 with ammoniacal liquor, and mixing is separated, obtains liquid and solid;
(3) liquid fermenting: the pH to 7.0 of the liquid that regulating step (2) obtains, access deposit number is the recombinant Saccharomyces cerevisiae (Saccharomycescerevisiae) of CGMCCNo.6634; Inoculum size is 15g/L; 26 DEG C, under rotating speed is the condition of 400rpm, cultivate 12h;
(4) enzymolysis: add pretreating raw material part B in the solid that step (2) obtains, mixing, regulates pH to 6.0, add cellulase and glucuroide, make cellulase be 30FPU/g, make glucuroide be 20CBU/g, at 60 DEG C of enzymolysis 12h;
(5) mixed fermentation: the enzymolysis solution that the fermented liquid obtain step (3) and step (4) obtain merges, mixing, regulates pH to 7.0; Temperature 37 DEG C, rotating speed is under the condition of 400rpm, cultivates 120h, obtains the fermented liquid being rich in ethanol.Alcohol yied is 69.8%.
The acid of the present embodiment step (1) is formic acid, and other same the present embodiment, obtains the fermented liquid being rich in ethanol.
Embodiment 4
Improve the method for xylose utilization in ligno-cellulose hydrolysate, comprise the steps:
(1)-(2) are with embodiment 1 (1)-(2);
(3) liquid fermenting: the pH to 6.0 of the liquid that regulating step (2) obtains, access deposit number is the recombinant Saccharomyces cerevisiae (Saccharomycescerevisiae) of CGMCCNo.6634; Inoculum size is 10g/L; At 30 DEG C, air flow is under the condition of 0.01vvm, cultivates 48h;
(4) with embodiment 1 (4);
(5) mixed fermentation: the enzymolysis solution that the fermented liquid obtain step (3) and step (4) obtain merges, mixing, regulates pH to 6.0; At 30 DEG C, air flow is under the condition of 0.01vvm, cultivates 90h, obtains the fermented liquid being rich in ethanol.Alcohol yied is 71.2%.
Embodiment 5
Improve the method for xylose utilization in ligno-cellulose hydrolysate, comprise the steps:
(1)-(2) are with embodiment 2 (1)-(2);
(3) liquid fermenting: the pH to 5.0 of the liquid that regulating step (2) obtains, access deposit number is the recombinant Saccharomyces cerevisiae (Saccharomycescerevisiae) of CGMCCNo.6634; Inoculum size is 1g/L; At 26 DEG C, air flow is under the condition of 0.005vvm, cultivates 48h;
(4) with embodiment 2 (4);
(5) mixed fermentation: the enzymolysis solution that the fermented liquid obtain step (3) and step (4) obtain merges, mixing, regulates pH to 5.0; At 26 DEG C, air flow is under the condition of 0.005vvm, cultivates 60h, obtains the fermented liquid being rich in ethanol.Alcohol yied is 66.8%.
Embodiment 6
Improve the method for xylose utilization in ligno-cellulose hydrolysate, comprise the steps:
(1)-(2) are with embodiment 2 (1)-(2);
(3) liquid fermenting: the pH to 7.0 of the liquid that regulating step (2) obtains, access deposit number is the recombinant Saccharomyces cerevisiae (Saccharomycescerevisiae) of CGMCCNo.6634; Inoculum size is 15g/L; At 37 DEG C, air flow is under the condition of 0.08vvm, cultivates 12h;
(4) with embodiment 3 (4);
(5) mixed fermentation: the enzymolysis solution that the fermented liquid obtain step (3) and step (4) obtain merges, mixing, regulates pH to 7.0; At 37 DEG C, air flow is under the condition of 0.08vvm, cultivates 120h, obtains the fermented liquid being rich in ethanol.Alcohol yied is 69.4%.
Claims (3)
1. improve the method for xylose utilization in ligno-cellulose hydrolysate, it is characterized in that comprising the steps:
(1) raw materials pretreatment: working concentration is that the aqueous acid of 5g/L ~ 50g/L soaks lignocellulosic material 10 ~ 24h, passes into water vapor, after making temperature rise to 180 ~ 220 DEG C, maintains 3 ~ 15 minutes, venting, cooling; Obtain pretreating raw material; The weight ratio of described lignocellulosic material dry weight and aqueous acid is 1:0.5 ~ 2; Pretreating raw material is divided into A, B two portions by weight the ratio for 1:0.2-1;
(2) water-washing pre-treatment raw material: get pretreating raw material part A and add water, makes the dry matter content of pretreating raw material part A be 10% ~ 20%, regulates pH to 4.0 ~ 6.0, and mixing is separated, obtains liquid and solid;
(3) liquid fermenting: pH to 5.0 ~ 7.0 of the liquid that regulating step (2) obtains, access deposit number is the recombinant Saccharomyces cerevisiae (Saccharomycescerevisiae) of CGMCCNo.6634; Inoculum size is 1 ~ 15g/L; 26 ~ 37 DEG C, under rotating speed is the condition of 100 ~ 400rpm, or at 26 ~ 37 DEG C, air flow is under the condition of 0.005vvm ~ 0.08vvm, cultivates 12-48h; ;
(4) enzymolysis: add pretreating raw material part B in the solid that step (2) obtains, mixing, regulate pH to 4.0 ~ 6.0, add cellulase and glucuroide, cellulase is made to be 10 ~ 30FPU/g, glucuroide is made to be 20 ~ 50CBU/g, at 30 ~ 60 DEG C of enzymolysis 12 ~ 48h;
(5) mixed fermentation: the enzymolysis solution that the fermented liquid obtain step (3) and step (4) obtain merges, mixing, regulates pH to 5.0 ~ 7.0; Temperature 26 ~ 37 DEG C, rotating speed is under the condition of 100 ~ 400rpm, or at 26 ~ 37 DEG C, air flow is under the condition of 0.005vvm ~ 0.08vvm, cultivates 60 ~ 120h, obtains the fermented liquid being rich in ethanol.
2. method according to claim 1, is characterized in that step (1) described acid is sulfuric acid, hydrochloric acid, oxalic acid or formic acid.
3. method according to claim 1, is characterized in that described lignocellulose is maize straw, broomcorn straw, bagasse, at least one in corn cob and wheat stalk.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107868802A (en) * | 2017-12-21 | 2018-04-03 | 叶芳 | A kind of method that bio-ethanol is prepared using agriculture and forestry organic waste material as raw material |
| CN107937446A (en) * | 2017-12-22 | 2018-04-20 | 天津大学 | A kind of technique using maize straw as waste ethanol |
| CN111057724A (en) * | 2019-12-10 | 2020-04-24 | 河北经贸大学 | Method for producing ethanol by fermentation of lignocellulose |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006128304A1 (en) * | 2005-06-03 | 2006-12-07 | Iogen Energy Corporation | Method of continuous processing of lignocellulosic feedstocks |
| CN101821398A (en) * | 2007-08-27 | 2010-09-01 | 埃欧金能源公司 | Method for the production of fermentation product from pretreated lignocellulosic feedstock |
| CN103060217A (en) * | 2012-11-29 | 2013-04-24 | 天津大学 | Recombinant yeast strain capable of efficiently metabolizing xylose and application thereof |
| CN104073525A (en) * | 2014-07-04 | 2014-10-01 | 天津大学 | Method for producing ethanol by virtue of simultaneous saccharification and co-fermentation |
| CN104789605A (en) * | 2015-02-10 | 2015-07-22 | 天津大学 | Method for fermentation production of ethanol in presence of inhibitor |
-
2016
- 2016-01-21 CN CN201610046770.9A patent/CN105567745A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006128304A1 (en) * | 2005-06-03 | 2006-12-07 | Iogen Energy Corporation | Method of continuous processing of lignocellulosic feedstocks |
| CN101821398A (en) * | 2007-08-27 | 2010-09-01 | 埃欧金能源公司 | Method for the production of fermentation product from pretreated lignocellulosic feedstock |
| CN103060217A (en) * | 2012-11-29 | 2013-04-24 | 天津大学 | Recombinant yeast strain capable of efficiently metabolizing xylose and application thereof |
| CN104073525A (en) * | 2014-07-04 | 2014-10-01 | 天津大学 | Method for producing ethanol by virtue of simultaneous saccharification and co-fermentation |
| CN104789605A (en) * | 2015-02-10 | 2015-07-22 | 天津大学 | Method for fermentation production of ethanol in presence of inhibitor |
Non-Patent Citations (3)
| Title |
|---|
| XUEBING ZHAO: "Organosolv pretreatment of lignocellulosic biomass", 《APPL MICROBIOL BIOTECHNOL》 * |
| 张秀男等: "木质纤维素原料糖化的预处理及脱毒方法研究进展", 《特产研究》 * |
| 罗鹏等: "酸催化的蒸汽爆破预处理强度对麦草酶水解影响的研究", 《林产化学与工业》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107868802A (en) * | 2017-12-21 | 2018-04-03 | 叶芳 | A kind of method that bio-ethanol is prepared using agriculture and forestry organic waste material as raw material |
| CN107937446A (en) * | 2017-12-22 | 2018-04-20 | 天津大学 | A kind of technique using maize straw as waste ethanol |
| CN111057724A (en) * | 2019-12-10 | 2020-04-24 | 河北经贸大学 | Method for producing ethanol by fermentation of lignocellulose |
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