CN103305556B - Method for producing ethanol by fermenting steam explosion quercus acutissima carr - Google Patents
Method for producing ethanol by fermenting steam explosion quercus acutissima carr Download PDFInfo
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- CN103305556B CN103305556B CN201310276378.XA CN201310276378A CN103305556B CN 103305556 B CN103305556 B CN 103305556B CN 201310276378 A CN201310276378 A CN 201310276378A CN 103305556 B CN103305556 B CN 103305556B
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- ethanol
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- acorn cup
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
The invention discloses a method for producing ethanol by fermenting steam explosion quercus acutissima carr, and the method comprises the following steps of: carrying out steam explosion pretreatment by taking quercus acutissima carr as a raw material; removing tannin contained in the quercus acutissima carr; hydrolyzing and liquifying cellulase; and fermenting to obtain the ethanol. The method disclosed by the invention can be used for producing the fuel ethanol by taking the forestry waste quercus acutissima carr as the raw material, carrying out raw material pretreatment and cellulase saccharification and then utilizing saccharomyces cerevisiae fermentation, thereby reducing the cost of the production process of the fuel ethanol and enhancing the economy of the production process of the fuel ethanol; and the method disclosed by the invention changes the wastes into valuables by producing the fuel ethanol by utilizing agriculture and forestry wastes, thereby solving the problem that fuel competes with human for grains, sufficiently utilizing the biomass resources, protecting the ecological environment and being favorable to realizing the sustainable development.
Description
Technical field
The present invention relates to the method for the quick-fried acorn cup producing fuel ethyl alcohol by ferment of the production method of alcohol fuel, particularly vapour.
Background technology
For many years, a large amount of uses of traditional energy result in energy dilemma and environmental degradation.In order to realize the Sustainable development of economy and environment, renewable, free of contamination biomass energy has become current study hotspot.
Lignocellulose is the renewable energy source material that on the earth, quantity is maximum, and whole world annual output is approximately 10,000,000,000 t according to the preliminary statistics.Utilize agriculture and forestry organic waste material as raw material production ethanol, realize the higher value application of biomass resource, be conducive to improving the ecological environment, meet the requirement of recycling economy development, the income of peasant can also be increased.
Acorn nut is the fruit of Fagaceae Quercus acutissima genus and evergreen sweet oak evergreen chinquapin class wild plant Oak Tree.The whole world has 8 genus more than 900 to plant, and be distributed in temperate zone, subtropics and the torrid zone, China accounts for 6 genus 301 kinds.There is abundant acorn nut resource in China, and acorn nut woods about has (1.3 ~ 1.67) × 10
7hm
2, 60 ~ 7,000,000,000 kilograms of acorn nuts can be produced every year.Acorn kernel both can be used as animal-feed, industrial raw material, also can be used to processing health food, take acorn starch as raw material production bio-fuel ethanol was also a good utilization ways, became the focus of research at present.And consequent acorn cup, output is large, source is wide, price is low, but cannot well be utilized, not only contaminate environment but also cause energy dissipation.Therefore, can consider to utilize cellulase hydrolysis saccharification acorn cup, thus make full use of biomass resource, preserve the ecological environment, realize Sustainable development.
Steam explosion adopts saturated vapor heating raw to certain pressure, high pressure steam infiltrated fiber is inner, discharge from blind hole gap in the mode of air-flow, make fiber that certain mechanical breaking occur, then sudden pressure reduction is to atmospheric biomass preprocessing means, is one of pretreated effective ways of lignocellulosic material.
Summary of the invention
The object of this invention is to provide the method for the quick-fried acorn cup fermentative production of ethanol of a kind of vapour, acorn cup is turned waste into wealth, reduce the production cost of alcohol fuel, improve the economy of the production process of alcohol fuel.
The present invention is by the following technical solutions:
A method for the quick-fried acorn cup fermentative production of ethanol of vapour, comprises the steps:
Step one, acorn cup to be dried, steam explosion is utilized to carry out pre-treatment, wherein, the ratio of acorn cup quality and volume of water is 1:10-1:20 (g:ml), the pressure of steam explosion is 1.0-2.0MPa, and the time length is 6-10min, is washed to neutrality afterwards, dry, pulverize, cross 40-~ 60 mesh sieve, obtained bursting charge powder;
Step 2, by bursting charge powder adopt ultrasonic cleaning remove tannin, bursting charge powder quality: the ratio of volume of water is 1:15-1:25 (g:20ml), ultrasonic temperature is 40-60 DEG C, ultrasonic time is 20-40min, ultrasonic power is 300W, filter afterwards, dry powder, obtain and remove tannin acorn cup powder;
Step 3, title remove tannin acorn cup powder, add citrate buffer solution, wherein, remove tannin acorn cup powder quality: the ratio of citrate buffer solution volume is 1g:10ml, be heated to 50 DEG C of preheating 20min, add cellulase according to 20FPU/g powder, concussion hydrolysis 48h at 50 DEG C, wherein citrate buffer solution is 0.1M, pH4.8, filters afterwards, collects hydrolyzed solution, being concentrated into sugared concentration is 60g/L, obtained cellulase hydrolysis saccharified liquid;
Step 4, by cellulase hydrolysis saccharified liquid, yeast extract 2g/L, ammonium sulfate 10g/L, potassium primary phosphate 2g/L, magnesium sulfate 2g/L is mixed with fermentation culture, inoculation 6-10%(v/v) fermentation by saccharomyces cerevisiae, afterwards filter obtain ethanol, wherein leavening temperature is 32 DEG C, fermentation rotating speed is 120r/min, and fermentation time is 68h.
Further, step 3 cellulase vigor is 15.6FPU/g.
Further, in step 4, yeast saccharomyces cerevisiae is the yeast culture liquid of the logarithmic phase 72h of PDA culture medium culturing.
Beneficial effect of the present invention: the inventive method with forestry waste acorn cup for raw material, after raw materials pretreatment and cellulase saccharification, utilize fermentation by saccharomyces cerevisiae to produce alcohol fuel, reduce the cost of fuel ethanol production process, improve the economy of the production process of alcohol fuel.The inventive method utilizes agriculture and forestry organic waste material to produce alcohol fuel, turns waste into wealth, and solves the problem that fuel and people strive grain, while can make full use of biomass resource, preserve the ecological environment, be conducive to realizing Sustainable development.
embodiment:
Below in conjunction with embodiment the present invention done and further explain.Should be appreciated that following examples are only intended to illustrate, should not be regarded as limitation of the scope of the invention.
Embodiment 1
By air-dry for acorn nut (picking up from Anhui Chuzhou) rear shelling, acorn cup 105 DEG C of dry for standby.Use steam explosion machine to carry out pre-treatment to acorn cup, the volume ratio of acorn cup quality and water is 1:20 (g:ml), and burstpressures is 1.0 MPa, 6 minutes time length.After process, with distilled water, raw material is washed till neutrality, 105 DEG C of oven dry, pulverizes with pulverizer, cross 40 ~ 60 mesh sieves.
Get quantitative bursting charge powder, add water by mass volume ratio 1:20 (g:ml), at 60 DEG C, power is 300W, ultrasonic cleaning 20min, then filters, 105 DEG C of oven dry, obtains and removes tannin acorn cup powder.
Take the tannin acorn cup powder that goes that above-mentioned steps obtains and add 0.1M, the citrate buffer solution of pH 4.8, solid-liquid ratio is 1:10 (g:ml), preheating 20 minutes in 50 DEG C of water-baths, then cellulase is added according to the enzyme amount of 20FPU/g powder, in 50 DEG C, 150 r/min are hydrolyzed 48h.After 48 hours, stop enzymic hydrolysis with ice cube immediately, filter, collect hydrolyzed solution.Hydrolyzed solution to be concentrated into sugared concentration be 60g/L. according to yeast saccharomyces cerevisiae kind liquid amass account for fermentation culture volume 6% inoculum size yeast saccharomyces cerevisiae kind liquid is inoculated in fermentation culture.Yeast saccharomyces cerevisiae kind liquid culture medium is PDA substratum, consists of potato 200g/L, glucose 20g/L, 121 DEG C of sterilizing 30min, and culture condition is 28 DEG C, incubation time 72h, obtains yeast saccharomyces cerevisiae kind liquid (planting liquid concentration is 1.5g/L).The formula of fermentation culture is: the cellulase hydrolysis saccharified liquid (sugared concentration is 60g/L) that above-mentioned steps obtains, yeast extract 2g/L, ammonium sulfate 10g/L, potassium primary phosphate 2g/L, magnesium sulfate 2g/L; Culture condition is 32 DEG C, rotating speed 120r/min, and fermentation time is 68h, and after fermentation ends, filtering fermentating liquid, utilizes gas-chromatography (analysis condition: AT capillary chromatographic column (0.53 mm × 18 m), column temperature 70 DEG C, column flow rate 6.36 mL/min.Fid detector, injection compartment temperature 180 DEG C, detector temperature 120 DEG C, sample size 1.0 μ L, carrier gas: nitrogen.) measure alcohol concn.In fermented liquid, alcohol concn is 25.63g/L after measured, and the sugar alcohol transformation efficiency in fermented liquid reaches 42.72%.
Embodiment 2
Getting oven dry acorn cup uses steam explosion machine to carry out pre-treatment, and the volume ratio of acorn cup quality and water is 1:10 (g:ml), and burstpressures is 2.0 MPa, 10 minutes time length.After process, with distilled water, raw material is washed till neutrality, 105 DEG C of oven dry, pulverizes with pulverizer, cross 40 ~ 60 mesh sieves, obtained bursting charge powder is stand-by.
Get quantitative bursting charge powder, add water by mass volume ratio 1:25 (g:ml), at 60 DEG C, power is 300W, ultrasonic cleaning 40min, then filters, 105 DEG C of oven dry, obtains and removes tannin acorn cup powder.
Take the tannin acorn cup powder that goes that above-mentioned steps obtains and add 0.1M, the citrate buffer solution of pH 4.8, the mass volume ratio of tannin acorn cup powder and citrate buffer solution is gone to be 1:10 (g:ml), preheating 20 minutes in 50 DEG C of water-baths, then cellulase is added according to the enzyme amount of 20FPU/g powder, in 50 DEG C, 150 r/min are hydrolyzed 48h.After 48 hours, stop enzymic hydrolysis with ice cube immediately, filter, collect hydrolyzed solution.Hydrolyzed solution being concentrated into sugared concentration is 60g/L, obtained cellulase hydrolysis saccharified liquid.
Yeast saccharomyces cerevisiae kind liquid is inoculated in fermentation culture by the inoculum size (v/v) by 10%.Yeast saccharomyces cerevisiae kind liquid and fermented liquid culture medium prescription are with embodiment 1.Culture condition is 32 DEG C, rotating speed 120r/min, and fermentation time is 68h, and after fermentation ends, filtering fermentating liquid, utilizes gas Chromatographic Determination alcohol concn.In fermented liquid, alcohol concn is 22.73g/L after measured, and the sugar alcohol transformation efficiency in fermented liquid reaches 37.89%.
Embodiment 3
Getting oven dry acorn cup uses steam explosion machine to carry out pre-treatment, and the volume ratio of acorn cup quality and water is 1:10 (g:ml), and burstpressures is 1.5 MPa, 8 minutes time length.After process, with distilled water, raw material is washed till neutrality, 105 DEG C of oven dry, pulverizes with pulverizer, cross 40 ~ 60 mesh sieves, obtained bursting charge powder is stand-by.
Get quantitative bursting charge powder, add water by mass volume ratio 1:20 (g:ml), at 50 DEG C, power is 300W, and cleaning 30min, then filters, 105 DEG C of oven dry, obtains and removes tannin acorn cup powder.
Take the tannin acorn cup powder that goes that above-mentioned steps obtains and add 0.1M, the citrate buffer solution of pH 4.8, the mass volume ratio of tannin acorn cup powder and citrate buffer solution is gone to be 1:10 (g:ml), preheating 20 minutes in 50 DEG C of water-baths, then cellulase is added according to the enzyme amount of 20FPU/g powder, in 50 DEG C, 150 r/min are hydrolyzed 48h.After 48 hours, stop enzymic hydrolysis with ice cube immediately, filter, collect hydrolyzed solution.Hydrolyzed solution being concentrated into sugared concentration is 60g/L, obtained cellulase hydrolysis saccharified liquid.
Yeast saccharomyces cerevisiae kind liquid is inoculated in fermentation culture by the inoculum size (v/v) by 8%.Yeast saccharomyces cerevisiae kind liquid and fermented liquid culture medium prescription are with embodiment 1.Culture condition is 32 DEG C, rotating speed 120r/min, and fermentation time is 68h, and after fermentation ends, filtering fermentating liquid, utilizes gas Chromatographic Determination alcohol concn.In fermented liquid, alcohol concn is 19.76g/L after measured, and the sugar alcohol transformation efficiency in fermented liquid reaches 32.94%.
Claims (3)
1. a method for the quick-fried acorn cup fermentative production of ethanol of vapour, is characterized in that, comprise the steps:
Step one, acorn cup to be dried, steam explosion is utilized to carry out pre-treatment, wherein, the ratio of acorn cup quality and volume of water is 1g:10-20ml, the pressure of steam explosion is 1.0-2.0MPa, and the time length is 6-10min, is washed to neutrality afterwards, dry, pulverize, cross 40-60 mesh sieve, obtained bursting charge powder;
Step 2, by bursting charge powder adopt ultrasonic cleaning remove tannin, wherein, the ratio of bursting charge powder quality and volume of water is 1g:20-25ml, ultrasonic temperature is 40-60 DEG C, and ultrasonic time is 20-40min, and ultrasonic power is 300W, filter afterwards, dry powder, obtain and remove tannin acorn cup powder;
Step 3, title remove tannin acorn cup powder, add citrate buffer solution, wherein, the ratio removing tannin acorn cup powder quality and citrate buffer solution volume is 1g:10ml, is heated to 50 DEG C of preheating 20min, cellulase is added according to 20FPU/g powder, at 50 DEG C, stop enzymic hydrolysis after concussion hydrolysis 48h, filter afterwards, collect hydrolyzed solution, being concentrated into sugared concentration is 60g/L, obtained cellulase hydrolysis saccharified liquid; Wherein citrate buffer solution is 0.1M, pH4.8;
Step 4, by cellulase hydrolysis saccharified liquid, yeast extract 2g/L, ammonium sulfate 10g/L, potassium primary phosphate 2g/L, magnesium sulfate 2g/L is mixed with fermentation culture, and the yeast saccharomyces cerevisiae kind liquid fermentation of inoculation 6-10 % v/v, filters afterwards and obtain ethanol; Wherein leavening temperature is 32 DEG C, and fermentation rotating speed is 120r/min, and fermentation time is 68h.
2. the method for the quick-fried acorn cup fermentative production of ethanol of vapour according to claim 1, is characterized in that, step 3 cellulase vigor is 15.6FPU/g.
3. the method for the quick-fried acorn cup fermentative production of ethanol of vapour according to claim 1, is characterized in that, in step 4, yeast saccharomyces cerevisiae is the yeast saccharomyces cerevisiae kind liquid of the logarithmic phase 72h of PDA culture medium culturing.
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| CN104404088A (en) * | 2014-12-26 | 2015-03-11 | 中国林业科学研究院林产化学工业研究所 | Method for preparing ethanol by performing alkaline pretreatment, enzymolysis and fermentation on forestry waste acorn shells |
| CN110964752A (en) * | 2019-12-30 | 2020-04-07 | 张静传 | Method for comprehensively utilizing acorns |
| CN110938661A (en) * | 2019-12-30 | 2020-03-31 | 张静传 | Method for producing ethanol from acorns |
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| CN101381744B (en) * | 2007-09-05 | 2012-05-23 | 中国科学院过程工程研究所 | Steam-explosion treatment of fermentation fuel ethanol by acorn fruit and comprehensive utilization method thereof |
| CN103103217B (en) * | 2011-11-10 | 2015-02-18 | 中国石油化工股份有限公司 | Production method for fuel ethanol |
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