CN102321671B - Method for biologic pretreatment of lignocellulose and hydrogen production through simultaneous saccharification and fermentation - Google Patents
Method for biologic pretreatment of lignocellulose and hydrogen production through simultaneous saccharification and fermentation Download PDFInfo
- Publication number
- CN102321671B CN102321671B CN 201110278172 CN201110278172A CN102321671B CN 102321671 B CN102321671 B CN 102321671B CN 201110278172 CN201110278172 CN 201110278172 CN 201110278172 A CN201110278172 A CN 201110278172A CN 102321671 B CN102321671 B CN 102321671B
- Authority
- CN
- China
- Prior art keywords
- fermentation
- lignocellulose
- liquid
- hydrogen
- simultaneous saccharification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for biologic pretreatment of lignocellulose and hydrogen production through simultaneous saccharification and fermentation, relates to a method for pre-treating lignocellulose and producing hydrogen through fermentation, and solves the problems of high energy requirement, environmental pollution and production of a fermentation inhibitor existing in the pretreatment of the lignocellulose during the conventional fermentative hydrogen production. The method comprises the following steps of: 1, inoculating white rot fungi in lignocellulose liquid culture medium to culture, washing and drying to obtain pretreated cellulose; and 2, mixing hydrogenogens nutritive salt solution and environmentally-friendly trichoderma rough enzyme solution, and adding into the pretreated cellulose to obtain simultaneous saccharification and fermentation hydrogen production culture medium; then introducing nitrogen; and inoculating seed liquid of hydrogenogens to perform anaerobic fermentation to produce hydrogen. By adopting the method, the energy consumption during lignocellulose hydrogen production is reduced, equipment investment is reduced, the fermentation inhibitor is not produced, damage probably caused to environment during hydrogen production is reduced to the minimum; the relative removal rate of lignin reaches 55.7 percent; and the hydrogen production capacity is 72.6ml/g.
Description
Technical field
The present invention relates to the method for a kind of preprocessing lignocellulose and fermentation and hydrogen production.
Background technology
At present, the world energy sources demand is mainly satisfied by fossil oil.Yet a large amount of scientific evidence show that the Global climate change that this random use fossil oil causes has potential devastating impact.Therefore, it is imperative to develop a kind of novel energy, and hydrogen has recoverable as a kind of novel energy carrier, cleanliness without any pollution and fuel value advantages of higher.The method of production hydrogen has a variety of, and wherein Fermentative Biohydrogen Production Process is paid close attention to widely with its higher hydrogen-producing speed and fermenting process energy-conserving and environment-protective.In order to make Hydrogen Bio-production By Anaerobic Fermentation have more economic competitiveness, it is crucial seeking a kind of renewable and cheap matrix.Lignocellulosic material is agricultural wastes for example, and waste paper and wood chip are one of renewable resourcess cheap, the abundantest on the earth.Asian countries has the huge potentiality of utilizing the agricultural wastes biological hydrogen production, and only the output of the annual agricultural wastes of China (maize straw, straw and wheat straw etc.) can reach about 700,000,000 tons, and energy equivalence is in 500,000,000 tons of mark coals.According to statistics, wherein, the stalk utilization ratio is 33%, but only accounts for 2.6% through what utilize after certain technical finesse, and all the other major parts just act as a fuel and wait directly utilization.Therefore, select straw-like materials as fermentation and hydrogen production matrix, DEVELOPMENT PROSPECT is boundless.
Adopt simultaneous saccharification and fermentation (Simultaneous Saccharification and Fermentation by lignocellulose to the conversion of hydrogen, SSF) technique is about to cellulosic Enzymatic hydrolysis process and hydrogen production through anaerobic fermentation and carries out synchronously saving time and improving space availability ratio.Because the spatial obstacle effect of provide protection, xylogen and the hemicellulose of stalk top layer wax and cellulosic high-crystallinity and the polymerization degree affect the biotransformation efficiency of stalk.Therefore, to fully effectively utilize agricultural straw resource, just must carry out pre-treatment to stalk, destroy the top layer wax of stalk, the covalent attachment of xylogen-hemicellulose, cellulosic crystalline texture etc., Mierocrystalline cellulose and xylogen and hemicellulose are separated from each other, increase the contact probability of cellulosic molecule and microorganism or enzyme, realize improving the purpose of straw biological transformation efficiency.Traditional physical/chemical pretreatment process is (such as acid, the pre-treatment of alkali High Temperature High Pressure, steam explosion, the explosion of ammonia fiber etc.) need expensive instrument or equipment, has higher energy demand, what generation was a large amount of in preprocessing process has inhibiting furfural class material to follow-up fermentation, it is high to lose simultaneously a large amount of Mierocrystalline celluloses and hemicellulose and whole preprocessing process cost, and environment is caused very big pollution.
Summary of the invention
Lignocellulose pre-treatment energy demand height in the existing fermentation and hydrogen production process, contaminate environment and the problem that produces fermentation inhibitor, and the method that provides a kind of Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation to produce hydrogen will be provided in the present invention.
The method that Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation produce hydrogen realizes according to the following steps:
One, white-rot fungi is inoculated in the lignocellulose liquid nutrient medium, cultivated 10～20 days at 29 ℃ aerobic conditions low suspensions, then washing places 105 ℃ of baking ovens to dry to constant weight again, Mierocrystalline cellulose after the acquisition pre-treatment; Two, 3L hydrogenogens nutrient salt solution is mixed with 7L viride crude enzyme liquid, Mierocrystalline cellulose after the pre-treatment of adding 150～350g, obtain simultaneous saccharification and fermentation and produce the hydrogen substratum, then pass into purity and be 99.99% nitrogen, inoculate the seed liquor of hydrogenogens, be 6.5 in initial pH value, temperature is that 60 ℃, stirring velocity are to carry out hydrogen production through anaerobic fermentation 24～72h under the condition of 150r/min; Namely finish Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation and produce hydrogen;
Wherein white-rot fungi is Phanerochaete chrysosporium5.776 in the step 1, and depositary institution is Chinese common micro-organisms culture presevation administrative center;
The every L of lignocellulose liquid nutrient medium is by the FeSO of 0.008g in the step 1
4, the yeast powder of 7.5g, the KH of 3g
2PO
4, 0.034g CaCl
2, 20g lignocellulose, the VITAMIN liquid of 1ml and the distilled water of surplus of glucose, 20g form; Described lignocellulose straw, wheat straw or corn stalk powder; Described VITAMIN liquid is dissolved in the 1000ml distilled water by the vitamin H of the PABA of the thiamine salt hydrochlorate of the pyridoxine hydrochloride of the pantothenic acid of the niacin of the inositol of 25g, 1g, 1g, 1g, 1g, 0.2g and 0.05g to be made;
The every L of hydrogenogens nutrient salt solution is by the ammonium chloride of 1.0g, the sodium-chlor of 1.0g, the dipotassium hydrogen phosphate of 3g, the potassium primary phosphate of 1.5g, the halfcystine of 0.5g, the MgCl of 0.5g in the step 2
26H
2The peptone of the Repone K of O, 0.2g, the yeast powder of 2g, 2g, the micro-metals of 1ml storage liquid, the VITAMIN storage liquid of 1ml and 0.1 ‰ (w/v) resazurin of 1ml form; The every L of described micro-metals storage liquid is by the iron protochloride of 1.5g, the zinc chloride of 70mg, the boric acid of 6mg, the MnCl of 0.1g
24H
2The CuCl of O, 2mg
22H
2The CoCl of O, 0.19g
26H
2The NiCl of O, 24mg
26H
2The Na of O, 36mg
2MO
4H
2The sodium wolframate of O, 15mg and the Na of 15mg
2SeO
45H
2O forms; The every L of described VITAMIN storage liquid is comprised of the Thioctic Acid of 50.0mg, the vitamin H of 20.0mg, the nicotinic acid of 0.35g, the vitamin of 5.0mg, the para-amino benzoic acid of 50.0mg, the folic acid of 20.0mg, the calcium pantothenate of 50.0mg, the vitamin B12 of 1.0mg and the pyridoxine hydrochloride of 100.0mg;
The preparation of the mould crude enzyme liquid of step 2 Green wood: viride is in the liquid culture medium, cultivated 4 days in 29 ℃, the aerobic conditions low suspension of 150r/min, then centrifugal 10min under 4 ℃, 8000r/min condition separates obtaining supernatant liquor and be the viride crude enzyme liquid; The spore inoculating amount of described viride is 1 * 10
12～1 * 10
16Individual/L, viride is Trichoderma viride3.2876, and separatrix is that sugar grinds 37, derives from Guangdong Province sugarcane Science Institute; The every L of described liquid culture medium is by the KH of 2.0g
2PO
4, 1.4g (NH
4)
2SO
4, 0.3g MgSO
47H
2The CaCl of O, 0.3g
2, 20g maize straw, the soybean cake powder of 5g, the micro-metals storage liquid of 1ml and the distilled water of surplus of wheat bran, 10g form; The every L of described micro-metals storage liquid is by the FeSO of 5.0g
47H
2The MgSO of O, 1.6g
4, 1.4g ZnSO
47H
2The CoCl of O, 2.0g
2Form with the distilled water of surplus;
The seed liquor of hydrogenogens is that inoculum size is 4% the liquid suspension liquid that grows to logarithmic phase in the step 2.
Advantage of the present invention: 1. use completely biological method to finish by the conversion process of lignocellulose to hydrogen, reduced the energy consumption in the lignocellulose product hydrogen process, reduce equipment investment, do not produce fermentation inhibitor, may be reduced to the destruction that environment causes minimum with producing the hydrogen process; 2. simultaneous saccharification and fermentation produces in the hydrogen substratum and has used the viride crude enzyme liquid that fiber is have higher degradation capability, it to pre-treatment after the Mierocrystalline cellulose saccharification that is hydrolyzed, conversion coefficient (conversion coefficient is the ratio of Mierocrystalline cellulose and hemicellulose in output of sugar and the Mierocrystalline cellulose) can reach 60-75%, do not adopt commercial enzyme, made the cost that produces hydrogen 60%; 3. the present invention adopts the white-rot fungi processing to carry out the pre-lignocellulose of biology and obtains preferably effect, and the relative clearance of xylogen reaches 55.7%, and hydrogen output is 72.6ml/g.
Description of drawings
Fig. 1 is the scanning electron microscope (SEM) photograph of the maize straw of white-rot fungi pre-treatment after 15 days among the present invention, and Fig. 2 is the scanning electron microscope (SEM) photograph of untreated maize straw.
Embodiment
Embodiment one: the method that present embodiment Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation produce hydrogen realizes according to the following steps:
One, white-rot fungi is inoculated in the lignocellulose liquid nutrient medium, cultivated 10～20 days at 29 ℃ aerobic conditions low suspensions, then washing places 105 ℃ of baking ovens to dry to constant weight again, Mierocrystalline cellulose after the acquisition pre-treatment; Two, 3L hydrogenogens nutrient salt solution is mixed with 7L viride crude enzyme liquid, Mierocrystalline cellulose after the pre-treatment of adding 150～350g, obtain simultaneous saccharification and fermentation and produce the hydrogen substratum, then pass into purity and be 99.99% nitrogen, inoculate the seed liquor of hydrogenogens, be 6.5 in initial pH value, temperature is that 60 ℃, stirring velocity are to carry out hydrogen production through anaerobic fermentation 24～72h under the condition of 150r/min; Namely finish Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation and produce hydrogen;
Wherein white-rot fungi is Phanerochaete chrysosporium5.776 in the step 1, and depositary institution is Chinese common micro-organisms culture presevation administrative center;
The every L of lignocellulose liquid nutrient medium is by the FeSO of 0.008g in the step 1
4, the yeast powder of 7.5g, the KH of 3g
2PO
4, 0.034g CaCl
2, 20g lignocellulose, the VITAMIN liquid of 1ml and the distilled water of surplus of glucose, 20g form; Described lignocellulose straw, wheat straw or corn stalk powder; Described VITAMIN liquid is dissolved in the 1000ml distilled water by the vitamin H of the PABA of the thiamine salt hydrochlorate of the pyridoxine hydrochloride of the pantothenic acid of the niacin of the inositol of 25g, 1g, 1g, 1g, 1g, 0.2g and 0.05g to be made;
The every L of hydrogenogens nutrient salt solution is by the ammonium chloride of 1.0g, the sodium-chlor of 1.0g, the dipotassium hydrogen phosphate of 3g, the potassium primary phosphate of 1.5g, the halfcystine of 0.5g, the MgCl of 0.5g in the step 2
26H
2The peptone of the Repone K of O, 0.2g, the yeast powder of 2g, 2g, the micro-metals of 1ml storage liquid, the VITAMIN storage liquid of 1ml and 0.1 ‰ (w/v) resazurin of 1ml form; The every L of described micro-metals storage liquid is by the iron protochloride of 1.5g, the zinc chloride of 70mg, the boric acid of 6mg, the MnCl of 0.1g
24H
2The CuCl of O, 2mg
22H
2The CoCl of O, 0.19g
26H
2The NiCl of O, 24mg
26H
2The Na of O, 36mg
2MO
4H
2The sodium wolframate of O, 15mg and the Na of 15mg
2SeO
45H
2O forms; The every L of described VITAMIN storage liquid is by the Thioctic Acid of 50.0mg, the vitamin H of 20.0mg, the nicotinic acid of 0.35g, the vitamin of 5.0mg, the para-amino benzoic acid of 50.0mg, the folic acid of 20.0mg, the calcium pantothenate of 50.0mg, the vitamins B of 1.0mg
12Form with the pyridoxine hydrochloride of 100.0mg;
The preparation of the mould crude enzyme liquid of step 2 Green wood: viride is in the liquid culture medium, cultivated 4 days in 29 ℃, the aerobic conditions low suspension of 150r/min, then centrifugal 10min under 4 ℃, 8000r/min condition separates obtaining supernatant liquor and be the viride crude enzyme liquid; The spore inoculating amount of described viride is 1 * 10
12～1 * 10
16Individual/L, viride is Trichoderma viride3.2876, and separatrix is that sugar grinds 37, derives from Guangdong Province sugarcane Science Institute; The every L of described liquid culture medium is by the KH of 2.0g
2PO
4, 1.4g (NH
4)
2SO
4, 0.3g MgSO
47H
2The CaCl of O, 0.3g
2, 20g maize straw, the soybean cake powder of 5g, the micro-metals storage liquid of 1ml and the distilled water of surplus of wheat bran, 10g form; The every L of described micro-metals storage liquid is by the FeSO of 5.0g
47H
2The MgSO of O, 1.6g
4, 1.4g ZnSO
47H
2The CoCl of O, 2.0g
2Form with the distilled water of surplus;
The seed liquor of hydrogenogens is that inoculum size is 4% the liquid suspension liquid that grows to logarithmic phase in the step 2.
In the present embodiment step 1 corn stalk powder described in the lignocellulose liquid nutrient medium be maize straw air-dry after, use pulverizer to pulverize 60 mesh sieves, and then dry to constant weight under 105 ℃.
Experiment:
One, white-rot fungi is inoculated in the lignocellulose liquid nutrient medium (employing corn stalk powder), cultivated 15 days at 29 ℃ aerobic conditions low suspensions, then washing places 105 ℃ of baking ovens to dry to constant weight again, Mierocrystalline cellulose after the acquisition pre-treatment; Two, 3L hydrogenogens nutrient salt solution is mixed with 7L viride crude enzyme liquid, Mierocrystalline cellulose after the pre-treatment of adding 150g, obtain simultaneous saccharification and fermentation and produce the hydrogen substratum, then pass into purity and be 99.99% nitrogen, inoculate the seed liquor of hydrogenogens, be 6.5 in initial pH value, temperature is that 60 ℃, stirring velocity are to carry out hydrogen production through anaerobic fermentation 24～72h under the condition of 150r/min; Namely finish Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation and produce hydrogen;
The result is, the maize straw of white-rot fungi pre-treatment after 15 days compares with untreated maize straw (seeing Fig. 2) as described in Figure 1, and as seen, untreated maize straw apparent structure is closely regular; And the maize straw lignocellulose structure of processing after 15 days is suffered serious destruction, there are a lot of cracks and cavity, this the explanation white rot fungus degrading a part of lignocellulose, infer according to figure, its degraded mainly be hemicellulose and xylogen, simultaneously can see that also cellulosic basic framework is not ruined, this explanation white-rot fungi is degraded to lignocellulose, but the cellulose amount of degraded seldom.Use the pretreated corn stalk fiber element of white-rot fungi to be separated from each other with xylogen and hemicellulose, increased the contact probability of cellulosic molecule and enzyme, realized improving the purpose of stalk enzymolysis efficiency.
Begin to have the gas output in the step 2 behind the 24h, the gas of output is detected through SC II type gas chromatograph (Shanghai analytical instrument) prove hydrogen; Gas stops output behind the 72h, is 72.6ml/g by calculating last as can be known hydrogen output, and the relative clearance of xylogen reaches 61.3%.
Embodiment two: present embodiment and embodiment one difference are: what the white-rot fungi inoculation was adopted in the step 1 is that concentration is 2 * 10
6The spore suspension of individual spore/ml, every bottle of inoculum size is 2 * 10
7Individual spore.Other step and parameter are identical with embodiment one.
Specifically third embodiment: The present embodiment differs from the specific first embodiment are: Step II seed liquid hydrogen producing bacteria in a hot solution of hydrogen producing bacteria Bacillus anaerobic glucose W16 (Thermoanaerobacterium? ThermosaccharolyticumW16), Thermoanaerobacterium? Thermosaccharolyticum? W16 in 2008 the first 33 pages of 6124-6132 "International? Journal? of? Hydrogen? Energy" in the name of the publication "Dark? fermentation? of? xylose? and? glucose? mix? using? isolated? Thermoanaerobacterium? thermosaccharolyticum ? W16 "article published.Other step and parameter are identical with embodiment one.
Claims (3)
1. a Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation produce the method for hydrogen, it is characterized in that the method that Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation produce hydrogen realizes according to the following steps:
One, white-rot fungi is inoculated in the lignocellulose liquid nutrient medium, cultivated 10～20 days at 29 ℃ aerobic conditions low suspensions, then washing places 105 ℃ of baking ovens to dry to constant weight again, Mierocrystalline cellulose after the acquisition pre-treatment; Two, 3L hydrogenogens nutrient salt solution is mixed with 7L viride crude enzyme liquid, Mierocrystalline cellulose after the pre-treatment of adding 150～350g, obtain simultaneous saccharification and fermentation and produce the hydrogen substratum, then pass into purity and be 99.99% nitrogen, inoculate the seed liquor of hydrogenogens, be 6.5 in initial pH value, temperature is that 60 ℃, stirring velocity are to carry out hydrogen production through anaerobic fermentation 24～72h under the condition of 150r/min; Namely finish Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation and produce hydrogen;
Wherein white-rot fungi is Phanerochaete chrysosporium 5.776 in the step 1;
The every L of lignocellulose liquid nutrient medium is by the FeSO of 0.008g in the step 1
4, the yeast powder of 7.5g, the KH of 3g
2PO
4, 0.034g CaCl
2, 20g lignocellulose, the VITAMIN liquid of 1ml and the distilled water of surplus of glucose, 20g form; Described lignocellulose is straw, wheat straw or corn stalk powder; Described VITAMIN liquid is dissolved in the 1000ml distilled water by the vitamin H of the PABA of the thiamine salt hydrochlorate of the pyridoxine hydrochloride of the niacin of the inositol of 25g, 1g, 1g pantothenic acid, 1g, 1g, 0.2g and 0.05g to be made;
The every L of hydrogenogens nutrient salt solution is by the ammonium chloride of 1.0g, the sodium-chlor of 1.0g, the dipotassium hydrogen phosphate of 3g, the potassium primary phosphate of 1.5g, the halfcystine of 0.5g, the MgCl of 0.5g in the step 2
26H
2The VITAMIN storage liquid of the peptone of the Repone K of O, 0.2g, the yeast powder of 2g, 2g, the micro-metals of 1ml storage liquid, 1ml and the mass body volume concentrations of 1ml are that 0.1 ‰ resazurins form; The every L of described micro-metals storage liquid is by the iron protochloride of 1.5g, the zinc chloride of 70mg, the boric acid of 6mg, the MnCl of 0.1g
24H
2The CuCl of O, 2mg
22H
2The CoCl of O, 0.19g
26H
2The NiCl of O, 24mg
26H
2The Na of O, 36mg
2MO
4H
2The sodium wolframate of O, 15mg and the Na of 15mg
2SeO
45H
2O forms; The every L of described VITAMIN storage liquid is by the Thioctic Acid of 50.0mg, the vitamin H of 20.0mg, the nicotinic acid of 0.35g, the vitamin of 5.0mg, the para-amino benzoic acid of 50.0mg, the folic acid of 20.0mg, the calcium pantothenate of 50.0mg, the vitamins B of 1.0mg
12Form with the pyridoxine hydrochloride of 100.0mg;
The preparation of the mould crude enzyme liquid of step 2 Green wood: viride is in the liquid culture medium, cultivated 4 days in 29 ℃, the aerobic conditions low suspension of 150r/min, then centrifugal 10min under 4 ℃, 8000r/min condition separates obtaining supernatant liquor and be the viride crude enzyme liquid; The spore inoculating amount of described viride is 1 * 10
12～1 * 10
16Individual/L, viride is Trichoderma viride 3.2876, and separatrix is that sugar grinds 37, derives from Guangdong Province sugarcane Science Institute; The every L of described liquid culture medium is by the KH of 2.0g
2PO
4, 1.4g (NH
4)
2SO
4, 0.3g MgSO
47H
2The CaCl of O, 0.3g
2, 20g maize straw, the soybean cake powder of 5g, the micro-metals storage liquid of 1ml and the distilled water of surplus of wheat bran, 10g form; The every L of described micro-metals storage liquid is by the FeSO of 5.0g
47H
2The MgSO of O, 1.6g
4, 1.4g ZnSO
47H
2The CoCl of O, 2.0g
2Form with the distilled water of surplus;
The seed liquor of hydrogenogens is that inoculum size is 4% the liquid suspension liquid that grows to logarithmic phase in the step 2.
2. a kind of Biological Pretreatment lignocellulose according to claim 1 and simultaneous saccharification and fermentation produce the method for hydrogen, and what it is characterized in that the white-rot fungi inoculation is adopted in the step 1 is that concentration is 2 * 10
6The spore suspension of individual spore/ml, every bottle of inoculum size is 2 * 10
7Individual spore.
3 according to claim 1, wherein a biological pretreatment and simultaneous saccharification and fermentation of lignocellulosic hydrogen production method, wherein the step two seeds in liquid hydrogen producing bacteria in the hydrogen producing bacteria is anaerobic bacillus pyrolysis sugar W16 (Thermoanaerobacterium? thermosaccharolyticum? W16), Thermoanaerobacterium? thermosaccharolyticum? W16 in 2008 the first 33 pages of 6124-6132 "International? Journal? of? Hydrogen? Energy", published in the name "Dark? fermentation? of? xylose ? and? glucose? mix? using? isolated? Thermoanaerobacterium? thermosaccharolyticum? W16 "article published.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110278172 CN102321671B (en) | 2011-09-19 | 2011-09-19 | Method for biologic pretreatment of lignocellulose and hydrogen production through simultaneous saccharification and fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110278172 CN102321671B (en) | 2011-09-19 | 2011-09-19 | Method for biologic pretreatment of lignocellulose and hydrogen production through simultaneous saccharification and fermentation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102321671A CN102321671A (en) | 2012-01-18 |
| CN102321671B true CN102321671B (en) | 2013-03-13 |
Family
ID=45449504
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110278172 Active CN102321671B (en) | 2011-09-19 | 2011-09-19 | Method for biologic pretreatment of lignocellulose and hydrogen production through simultaneous saccharification and fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102321671B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106609294B (en) * | 2015-10-22 | 2020-08-28 | 中国科学院过程工程研究所 | Method for producing hydrogen by strengthening double-bacterium fermentation of cellulose |
| CN106635887B (en) * | 2016-11-21 | 2019-10-18 | 华南理工大学 | A kind of thermophilic solution sugar anaerobic bacillus(cillus anaerobicus) and its application in biological hydrogen production |
| CN106554974B (en) * | 2016-11-28 | 2021-02-19 | 东北大学 | Method for producing hydrogen by fermentation by using modified peanut shells as supplementary substrate |
| CN108531515A (en) * | 2018-04-27 | 2018-09-14 | 哈尔滨工业大学 | A kind of method of fungi preprocessing lignocellulose and direct microorganism conversion fermentation and hydrogen production |
| CN109362813A (en) * | 2018-11-05 | 2019-02-22 | 贵州好菇粮农业科技有限公司 | A kind of mushroom growth regulator and preparation method thereof |
| CN109355315B (en) * | 2018-11-19 | 2022-07-26 | 华南理工大学 | Method for producing ethanol and succinic acid by taking bagasse as raw material and synchronous fermentation and application |
| CN110484571B (en) * | 2019-09-29 | 2023-04-25 | 哈尔滨工业大学 | Method for semi-continuously producing hydrogen and grease by using corn straw |
| CN112852683B (en) * | 2021-03-24 | 2022-10-25 | 华南理工大学 | Thermosaccharophilus anaerobacter and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101008018A (en) * | 2007-01-26 | 2007-08-01 | 哈尔滨工业大学 | Method for mixed bacterial strain to degrade and ferment cellulose for producing hydrogen |
| CN101402972A (en) * | 2008-11-18 | 2009-04-08 | 哈尔滨工业大学 | Method for decomposing cellulose to produce hydrogen with cellulose clostridium |
-
2011
- 2011-09-19 CN CN 201110278172 patent/CN102321671B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101008018A (en) * | 2007-01-26 | 2007-08-01 | 哈尔滨工业大学 | Method for mixed bacterial strain to degrade and ferment cellulose for producing hydrogen |
| CN101402972A (en) * | 2008-11-18 | 2009-04-08 | 哈尔滨工业大学 | Method for decomposing cellulose to produce hydrogen with cellulose clostridium |
Non-Patent Citations (2)
| Title |
|---|
| 曹广丽.高效利用玉米秸秆的产氢菌种及其产氢性能研究.《中国博士学位论文全文数据库》.2011,(第4期),全文. |
| 高效利用玉米秸秆的产氢菌种及其产氢性能研究;曹广丽;《中国博士学位论文全文数据库》;20110415(第4期);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102321671A (en) | 2012-01-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102321671B (en) | Method for biologic pretreatment of lignocellulose and hydrogen production through simultaneous saccharification and fermentation | |
| Maslova et al. | Production of various organic acids from different renewable sources by immobilized cells in the regimes of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SFF) | |
| Chen et al. | Enzymatic hydrolysis of corncob and ethanol production from cellulosic hydrolysate | |
| CN103667110B (en) | One bacillus coagulans and use this bacterium synchronous saccharification altogether fermenting lignocellulose to produce the integrated technique of lactic acid | |
| Srivastava et al. | Application of cellulases in biofuels industries: an overview | |
| Castro et al. | Selection of a thermotolerant Kluyveromyces marxianus strain with potential application for cellulosic ethanol production by simultaneous saccharification and fermentation | |
| CN102719371B (en) | Clostridium beijerinckii and method for preparing biological butanol through fermentation of xylose residue serving as raw material thereof | |
| CN102199554B (en) | Saccharomyces cerevisiae strain with multiple-stress resistance, and application thereof in cellulose alcohol fermentation | |
| CN104774877B (en) | A kind of method of lignocellulose biomass co-producing ethanol, acetone and butanol | |
| CN102174433B (en) | Clostridium beijerinckii with high stress resistance and application thereof | |
| Lin et al. | Evaluation of spent mushroom compost as a lignocellulosic substrate for hydrogen production by Clostridium thermocellum | |
| CN102660519B (en) | Method for preparing biological enzyme by utilizing fermentation waste liquid | |
| CN102154371B (en) | Method for producing hydrogen by straw | |
| Liu et al. | Consolidated bioprocess for bioethanol production with alkali-pretreated sugarcane bagasse | |
| CN105200094B (en) | A method of utilizing microbial fermentation lignocellulosic material producing and ethanol | |
| CN106636226A (en) | Method for fermentation preparation of butanol by using lignocellulose as raw material | |
| CN103571772B (en) | The method of the product butanols bacterial strain that one strain is new and production butanols thereof | |
| CN103993042A (en) | Method for combined production of bioethanol and pullulan from lignocellulose substances | |
| CN101671645B (en) | Method for preparing beta-mannase and special strain thereof | |
| CN102925365B (en) | Trichoderma atroviride strain and application thereof in preparation of cellulase | |
| CN106957876A (en) | A kind of method that utilization lignocellulosic material fermentation prepares butanol | |
| CN104805133B (en) | A method of ethyl alcohol is produced using microorganism common fermentation C5 and C6 | |
| CN103060418A (en) | Method of constructing mixed bacteria system for fermenting straw stalks to produce ethanol | |
| CN105713851B (en) | One plant of Clostridium beijerinckii and its application | |
| Tang et al. | Evaluation of hydrogen production from corn cob with the mesophilic bacterium Clostridium hydrogeniproducens HR-1 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |