CN102321671A - Method for biologic pretreatment of lignocellulose and hydrogen production through simultaneous saccharification and fermentation - Google Patents
Method for biologic pretreatment of lignocellulose and hydrogen production through simultaneous saccharification and fermentation Download PDFInfo
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Abstract
The invention discloses a method for biologic pretreatment of lignocellulose and hydrogen production through simultaneous saccharification and fermentation, relates to a method for pre-treating lignocellulose and producing hydrogen through fermentation, and solves the problems of high energy requirement, environmental pollution and production of a fermentation inhibitor existing in the pretreatment of the lignocellulose during the conventional fermentative hydrogen production. The method comprises the following steps of: 1, inoculating white rot fungi in lignocellulose liquid culture medium to culture, washing and drying to obtain pretreated cellulose; and 2, mixing hydrogenogens nutritive salt solution and environmentally-friendly trichoderma rough enzyme solution, and adding into the pretreated cellulose to obtain simultaneous saccharification and fermentation hydrogen production culture medium; then introducing nitrogen; and inoculating seed liquid of hydrogenogens to perform anaerobic fermentation to produce hydrogen. By adopting the method, the energy consumption during lignocellulose hydrogen production is reduced, equipment investment is reduced, the fermentation inhibitor is not produced, damage probably caused to environment during hydrogen production is reduced to the minimum; the relative removal rate of lignin reaches 55.7 percent; and the hydrogen production capacity is 72.6ml/g.
Description
Technical field
The present invention relates to the method for a kind of preprocessing lignocellulose and fermentation and hydrogen production.
Background technology
At present, the world energy sources demand is mainly satisfied by fossil oil.Yet a large amount of scientific evidence show that the Global climate change that this random use fossil oil causes has the potential devastating impact.Therefore, it is imperative to develop a kind of novel energy, and hydrogen has recyclable utilization as a kind of novel energy carrier, cleanliness without any pollution and fuel value advantages of higher.The method of production hydrogen has a variety of, and wherein the fermentation method bio-hydrogen production technology receives widely and paying close attention to its higher hydrogen-producing speed and fermenting process energy-conserving and environment-protective.In order to make the fermentation method biological hydrogen production have more economic competitiveness, it is crucial seeking a kind of renewable and cheap matrix.Lignocellulosic material is agricultural wastes for example, and waste paper and wood chip are one of renewable resourcess the most cheap, the abundantest on the earth.Asian countries has the huge potentiality of utilizing the agricultural wastes biological hydrogen production, and only the output of the annual agricultural wastes of China (corn straw, straw and wheat straw etc.) can reach about 700,000,000 tons, and energy is equivalent to 500,000,000 tons of mark coals.According to statistics, wherein, stalk class utilization ratio is 33%, but only accounts for 2.6% through what utilize after certain technical finesse, and all the other major parts just act as a fuel and wait directly utilization.Therefore, select for use straw-like materials as fermentation and hydrogen production matrix, DEVELOPMENT PROSPECT is boundless.
Adopt simultaneous saccharification and fermentation (Simultaneous Saccharification and Fermentation by lignocellulose to the conversion of hydrogen; SSF) technology is about to cellulosic enzymic hydrolysis saccharifying and hydrogen production through anaerobic fermentation and carries out synchronously to save time and to improve space availability ratio.Because the spatial obstacle effect of provide protection, xylogen and the semicellulose of stalk top layer wax and cellulosic high-crystallinity and the polymerization degree influence the bio-transformation efficient of stalk.Therefore; To fully effectively utilize agricultural straw resource; Just must carry out pre-treatment, destroy the top layer wax of stalk, the covalent attachment of xylogen-semicellulose, cellulosic crystalline texture etc., Mierocrystalline cellulose and xylogen and semicellulose are separated from each other stalk; Increase the contact probability of cellulosic molecule and mikrobe or enzyme, realize improving the purpose of straw biological transformation efficiency.Traditional physical/chemical pretreatment process is (like acid, the pre-treatment of alkali HTHP; Steam explosion, the explosion of ammonia fiber etc.) need expensive instrument or equipment; Has higher energy demand; What generation was a large amount of in preprocessing process has inhibiting furfural class material to follow-up fermentation, and it is high to lose a large amount of Mierocrystalline celluloses and semicellulose and whole preprocessing process cost simultaneously, and environment is caused very big pollution.
Summary of the invention
The present invention will solve lignocellulose pre-treatment energy demand height in the existing fermentation and hydrogen production process, contaminate environment and the problem that produces fermentation inhibitor, and the method that provides a kind of Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation to produce hydrogen.
The method that Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation produce hydrogen realizes according to the following steps:
One, white-rot fungi is inoculated in the lignocellulose liquid nutrient medium, cultivated 10～20 days at 29 ℃ aerobic conditions low suspensions, washing places 105 ℃ of baking ovens to dry to constant weight more then, Mierocrystalline cellulose after the acquisition pre-treatment; Two, 3L hydrogenogens nutrient salt solution is mixed with 7L viride crude enzyme liquid; Mierocrystalline cellulose after the pre-treatment of adding 150～350g; Obtain simultaneous saccharification and fermentation and produce the hydrogen substratum; Feed purity then and be 99.99% nitrogen, inoculate the seed liquor of hydrogenogens, be 6.5 in original ph, temperature is that 60 ℃, stirring velocity are to carry out hydrogen production through anaerobic fermentation 24～72h under the condition of 150r/min; Promptly accomplish Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation and produce hydrogen;
Wherein white-rot fungi is Phanerochaete chrysosporium5.776 in the step 1, and depositary institution is a Chinese common micro-organisms culture presevation administrative center;
The every L of lignocellulose liquid nutrient medium is by the FeSO of 0.008g in the step 1
4, the yeast powder of 7.5g, the KH of 3g
2PO
4, 0.034g CaCl
2, 20g lignocellulose, the VITAMINs liquid of 1ml and the zero(ppm) water of surplus of glucose, 20g form; Said lignocellulose straw, wheat straw or corn stalk powder; Said VITAMINs liquid is dissolved in the 1000ml zero(ppm) water by the vitamin H of the 4-benzaminic acid of the thiamine salt hydrochlorate of the pyridoxine hydrochloride of the pantothenic acid of the niacin of the inositol of 25g, 1g, 1g, 1g, 1g, 0.2g and 0.05g to be processed;
The every L of hydrogenogens nutrient salt solution is by the ammonium chloride of 1.0g, the sodium-chlor of 1.0g, the potassium hydrogenphosphate of 3g, the potassium primary phosphate of 1.5g, the halfcystine of 0.5g, the MgCl of 0.5g in the step 2
26H
2The peptone of the Repone K of O, 0.2g, the yeast powder of 2g, 2g, the micro-metals of 1ml storage liquid, the VITAMINs storage liquid of 1ml and 0.1 ‰ (w/v) resazurin of 1ml are formed; The every L of said micro-metals storage liquid is by the iron protochloride of 1.5g, the zinc chloride of 70mg, the boric acid of 6mg, the MnCl of 0.1g
24H
2The CuCl of O, 2mg
22H
2The CoCl of O, 0.19g
26H
2The NiCl of O, 24mg
26H
2The Na of O, 36mg
2MO
4H
2The sodium wolframate of O, 15mg and the Na of 15mg
2SeO
45H
2O forms; The every L of said VITAMINs storage liquid is made up of the Thioctic Acid of 50.0mg, the vitamin H of 20.0mg, the nicotinic acid of 0.35g, the vitamin of 5.0mg, the para-amino benzoic acid of 50.0mg, the folic acid of 20.0mg, the VA of 50.0mg, the cobalamin of 1.0mg and the pyridoxine hydrochloride of 100.0mg;
The preparation of viride crude enzyme liquid in the step 2: viride produces in the enzyme substratum at liquid; Cultivated 4 days in 29 ℃, the aerobic conditions low suspension of 150r/min; Centrifugal 10min under 4 ℃, 8000r/min condition then separates obtaining supernatant and be the viride crude enzyme liquid; The spore inoculating amount of said viride is 1 * 10
12～1 * 10
16Individual/L, viride is Trichoderma viride3.2876, and separatrix is that sugar grinds 37, derives from Guangdong Province sugarcane Science Institute; Described liquid produces the KH of the every L of enzyme substratum by 2.0g
2PO
4, 1.4g (NH
4)
2SO
4, 0.3g MgSO
47H
2The CaCl of O, 0.3g
2, 20g corn straw, the soybean cake powder of 5g, the micro-metals storage liquid of 1ml and the zero(ppm) water of surplus of wheat bran, 10g form; The every L of described micro-metals storage liquid is by the FeSO of 5.0g
47H
2The MgSO of O, 1.6g
4, 1.4g ZnSO
47H
2The CoCl of O, 2.0g
2Form with the zero(ppm) water of surplus;
The seed liquor of hydrogenogens is that inoculum size is 4% the liquid suspension liquid that grows to logarithmic phase in the step 2.
Advantage of the present invention: 1. use biological method completion completely by the conversion process of lignocellulose to hydrogen; Reduce lignocellulose and produced the energy consumption in the hydrogen process; Minimizing equipment drops into, and does not produce fermentation inhibitor, possibly be reduced to minimum to the destruction that environment causes with producing the hydrogen process; 2. simultaneous saccharification and fermentation produces in the hydrogen substratum and has used the viride crude enzyme liquid that fiber is have higher degradation capability; It to pre-treatment after the Mierocrystalline cellulose saccharification that is hydrolyzed; Conversion coefficient (conversion coefficient is the ratio of Mierocrystalline cellulose and semicellulose in output of sugar and the Mierocrystalline cellulose) can reach 60-75%; Do not adopt commercial enzyme, make the cost that produces hydrogen reduce by 60%; 3. the present invention adopts the preparatory lignocellulose of white-rot fungi processing carrying out biology to obtain effect preferably, and the relative clearance of xylogen reaches 55.7%, and hydrogen output is 72.6ml/g.
Description of drawings
Fig. 1 is the sem photograph of the corn straw of white-rot fungi pre-treatment after 15 days among the present invention, and Fig. 2 is the sem photograph of untreated corn straw.
Embodiment
Embodiment one: the method that this embodiment Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation produce hydrogen realizes according to the following steps:
One, white-rot fungi is inoculated in the lignocellulose liquid nutrient medium, cultivated 10～20 days at 29 ℃ aerobic conditions low suspensions, washing places 105 ℃ of baking ovens to dry to constant weight more then, Mierocrystalline cellulose after the acquisition pre-treatment; Two, 3L hydrogenogens nutrient salt solution is mixed with 7L viride crude enzyme liquid; Mierocrystalline cellulose after the pre-treatment of adding 150～350g; Obtain simultaneous saccharification and fermentation and produce the hydrogen substratum; Feed purity then and be 99.99% nitrogen, inoculate the seed liquor of hydrogenogens, be 6.5 in original ph, temperature is that 60 ℃, stirring velocity are to carry out hydrogen production through anaerobic fermentation 24～72h under the condition of 150r/min; Promptly accomplish Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation and produce hydrogen;
Wherein white-rot fungi is Phanerochaete chrysosporium5.776 in the step 1, and depositary institution is a Chinese common micro-organisms culture presevation administrative center;
The every L of lignocellulose liquid nutrient medium is by the FeSO of 0.008g in the step 1
4, the yeast powder of 7.5g, the KH of 3g
2PO
4, 0.034g CaCl
2, 20g lignocellulose, the VITAMINs liquid of 1ml and the zero(ppm) water of surplus of glucose, 20g form; Said lignocellulose straw, wheat straw or corn stalk powder; Said VITAMINs liquid is dissolved in the 1000ml zero(ppm) water by the vitamin H of the 4-benzaminic acid of the thiamine salt hydrochlorate of the pyridoxine hydrochloride of the pantothenic acid of the niacin of the inositol of 25g, 1g, 1g, 1g, 1g, 0.2g and 0.05g to be processed;
The every L of hydrogenogens nutrient salt solution is by the ammonium chloride of 1.0g, the sodium-chlor of 1.0g, the potassium hydrogenphosphate of 3g, the potassium primary phosphate of 1.5g, the halfcystine of 0.5g, the MgCl of 0.5g in the step 2
26H
2The peptone of the Repone K of O, 0.2g, the yeast powder of 2g, 2g, the micro-metals of 1ml storage liquid, the VITAMINs storage liquid of 1ml and 0.1 ‰ (w/v) resazurin of 1ml are formed; The every L of said micro-metals storage liquid is by the iron protochloride of 1.5g, the zinc chloride of 70mg, the boric acid of 6mg, the MnCl of 0.1g
24H
2The CuCl of O, 2mg
22H
2The CoCl of O, 0.19g
26H
2The NiCl of O, 24mg
26H
2The Na of O, 36mg
2MO
4H
2The sodium wolframate of O, 15mg and the Na of 15mg
2SeO
45H
2O forms; The every L of said VITAMINs storage liquid is by the Thioctic Acid of 50.0mg, the vitamin H of 20.0mg, the nicotinic acid of 0.35g, the vitamin of 5.0mg, the para-amino benzoic acid of 50.0mg, the folic acid of 20.0mg, the VA of 50.0mg, the vitamins B of 1.0mg
12Form with the pyridoxine hydrochloride of 100.0mg;
The preparation of viride crude enzyme liquid in the step 2: viride produces in the enzyme substratum at liquid; Cultivated 4 days in 29 ℃, the aerobic conditions low suspension of 150r/min; Centrifugal 10min under 4 ℃, 8000r/min condition then separates obtaining supernatant and be the viride crude enzyme liquid; The spore inoculating amount of said viride is 1 * 10
12～1 * 10
16Individual/L, viride is Trichoderma viride3.2876, and separatrix is that sugar grinds 37, derives from Guangdong Province sugarcane Science Institute; Described liquid produces the KH of the every L of enzyme substratum by 2.0g
2PO
4, 1.4g (NH
4)
2SO
4, 0.3g MgSO
47H
2The CaCl of O, 0.3g
2, 20g corn straw, the soybean cake powder of 5g, the micro-metals storage liquid of 1ml and the zero(ppm) water of surplus of wheat bran, 10g form; The every L of described micro-metals storage liquid is by the FeSO of 5.0g
47H
2The MgSO of O, 1.6g
4, 1.4g ZnSO
47H
2The CoCl of O, 2.0g
2Form with the zero(ppm) water of surplus;
The seed liquor of hydrogenogens is that inoculum size is 4% the liquid suspension liquid that grows to logarithmic phase in the step 2.
In this embodiment step 1 the corn stalk powder described in the lignocellulose liquid nutrient medium be corn straw air-dry after, use kibbler to pulverize 60 mesh sieves, and then dry to constant weight under 105 ℃.
Experiment:
One, white-rot fungi is inoculated in the lignocellulose liquid nutrient medium (employing corn stalk powder), cultivated 15 days at 29 ℃ aerobic conditions low suspensions, washing places 105 ℃ of baking ovens to dry to constant weight more then, Mierocrystalline cellulose after the acquisition pre-treatment; Two, 3L hydrogenogens nutrient salt solution is mixed with 7L viride crude enzyme liquid; Mierocrystalline cellulose after the pre-treatment of adding 150g; Obtain simultaneous saccharification and fermentation and produce the hydrogen substratum; Feed purity then and be 99.99% nitrogen, inoculate the seed liquor of hydrogenogens, be 6.5 in original ph, temperature is that 60 ℃, stirring velocity are to carry out hydrogen production through anaerobic fermentation 24～72h under the condition of 150r/min; Promptly accomplish Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation and produce hydrogen;
The result is that corn straw such as the Fig. 1 after 15 days is said in the white-rot fungi pre-treatment, compares with untreated corn straw (see figure 2), it is thus clear that untreated corn straw apparent structure is closely regular; And the corn straw lignocellulose structure of handling after 15 days is suffered serious destruction, has a lot of cracks and cavity, this explanation white rot fungus degrading a part of lignocellulose; Infer according to figure; Its degraded mainly be semicellulose and xylogen, simultaneously can see that also cellulosic basic framework is not ruined; This explanation white-rot fungi is degraded to lignocellulose, but the cellulose amount of degraded seldom.Use pretreated corn straw Mierocrystalline cellulose of white-rot fungi and xylogen and semicellulose to be separated from each other, increased the contact probability of cellulosic molecule and enzyme, realized improving the purpose of stalk enzymolysis efficiency.
Begin to have the gas output in the step 2 behind the 24h, the gas of output is detected through SC II type gas chromatograph (Shanghai analytical instrument) prove hydrogen; Gas stops output behind the 72h, can know that through calculating last hydrogen output is 72.6ml/g, and the relative clearance of xylogen reaches 61.3%.
Embodiment two: this embodiment with embodiment one difference is: what the white-rot fungi inoculation was adopted in the step 1 is that concentration is 2 * 10
6The spore suspension of individual spore/ml, every bottle of inoculum size is 2 * 10
7Individual spore.Other step and parameter are identical with embodiment one.
Embodiment three: this embodiment and embodiment one difference are: the hydrogenogens in the step 2 in the seed liquor of hydrogenogens is pyrolysis sugar anaerobic spore-bearing bacilli W16 (Thermoanaerobacterium thermosaccharolyticumW16), discloses in the article that is called " Dark fermentation of xylose and glucose mix using isolated Thermoanaerobacterium thermosaccharolyticum W16 " that Thermoanaerobacterium thermosaccharolyticum W16 publish in " the International Journal of Hydrogen Energy " of 2008 the 33rd phase 6124-6132 pages or leaves.Other step and parameter are identical with embodiment one.
Claims (3)
1. Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation produce the method for hydrogen, it is characterized in that the method that Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation produce hydrogen realizes according to the following steps:
One, white-rot fungi is inoculated in the lignocellulose liquid nutrient medium, cultivated 10～20 days at 29 ℃ aerobic conditions low suspensions, washing places 105 ℃ of baking ovens to dry to constant weight more then, Mierocrystalline cellulose after the acquisition pre-treatment; Two, 3L hydrogenogens nutrient salt solution is mixed with 7L viride crude enzyme liquid; Mierocrystalline cellulose after the pre-treatment of adding 150～350g; Obtain simultaneous saccharification and fermentation and produce the hydrogen substratum; Feed purity then and be 99.99% nitrogen, inoculate the seed liquor of hydrogenogens, be 6.5 in original ph, temperature is that 60 ℃, stirring velocity are to carry out hydrogen production through anaerobic fermentation 24～72h under the condition of 150r/min; Promptly accomplish Biological Pretreatment lignocellulose and simultaneous saccharification and fermentation and produce hydrogen;
Wherein white-rot fungi is Phanerochaete chrysosporium5.776 in the step 1;
The every L of lignocellulose liquid nutrient medium is by the FeSO of 0.008g in the step 1
4, the yeast powder of 7.5g, the KH of 3g
2PO
4, 0.034g CaCl
2, 20g lignocellulose, the VITAMINs liquid of 1ml and the zero(ppm) water of surplus of glucose, 20g form; Said lignocellulose straw, wheat straw or corn stalk powder; Said VITAMINs liquid is dissolved in the 1000ml zero(ppm) water by the vitamin H of the 4-benzaminic acid of the thiamine salt hydrochlorate of the pyridoxine hydrochloride of the pantothenic acid of the niacin of the inositol of 25g, 1g, 1g, 1g, 1g, 0.2g and 0.05g to be processed;
The every L of hydrogenogens nutrient salt solution is by the ammonium chloride of 1.0g, the sodium-chlor of 1.0g, the potassium hydrogenphosphate of 3g, the potassium primary phosphate of 1.5g, the halfcystine of 0.5g, the MgCl of 0.5g in the step 2
26H
2The peptone of the Repone K of O, 0.2g, the yeast powder of 2g, 2g, the micro-metals of 1ml storage liquid, the VITAMINs storage liquid of 1ml and 0.1 ‰ (w/v) resazurin of 1ml are formed; The every L of said micro-metals storage liquid is by the iron protochloride of 1.5g, the zinc chloride of 70mg, the boric acid of 6mg, the MnCl of 0.1g
24H
2The CuCl of O, 2mg
22H
2The CoCl of O, 0.19g
26H
2The NiCl of O, 24mg
26H
2The Na of O, 36mg
2MO
4H
2The sodium wolframate of O, 15mg and the Na of 15mg
2SeO
45H
2O forms; The every L of said VITAMINs storage liquid is by the Thioctic Acid of 50.0mg, the vitamin H of 20.0mg, the nicotinic acid of 0.35g, the vitamin of 5.0mg, the para-amino benzoic acid of 50.0mg, the folic acid of 20.0mg, the VA of 50.0mg, the vitamins B of 1.0mg
12Form with the pyridoxine hydrochloride of 100.0mg;
The preparation of viride crude enzyme liquid in the step 2: viride produces in the enzyme substratum at liquid; Cultivated 4 days in 29 ℃, the aerobic conditions low suspension of 150r/min; Centrifugal 10min under 4 ℃, 8000r/min condition then separates obtaining supernatant and be the viride crude enzyme liquid; The spore inoculating amount of said viride is 1 * 10
12～1 * 10
16Individual/L, viride is Trichoderma viride3.2876, and separatrix is that sugar grinds 37, derives from Guangdong Province sugarcane Science Institute; Described liquid produces the KH of the every L of enzyme substratum by 2.0g
2PO
4, 1.4g (NH
4)
2SO
4, 0.3g MgSO
47H
2The CaCl of O, 0.3g
2, 20g corn straw, the soybean cake powder of 5g, the micro-metals storage liquid of 1ml and the zero(ppm) water of surplus of wheat bran, 10g form; The every L of described micro-metals storage liquid is by the FeSO of 5.0g
47H
2The MgSO of O, 1.6g
4, 1.4g ZnSO
47H
2The CoCl of O, 2.0g
2Form with the zero(ppm) water of surplus;
The seed liquor of hydrogenogens is that inoculum size is 4% the liquid suspension liquid that grows to logarithmic phase in the step 2.
2. a kind of Biological Pretreatment lignocellulose according to claim 1 and simultaneous saccharification and fermentation produce the method for hydrogen, and what it is characterized in that the white-rot fungi inoculation is adopted in the step 1 is that concentration is 2 * 10
6The spore suspension of individual spore/ml, every bottle of inoculum size is 2 * 10
7Individual spore.
3. a kind of Biological Pretreatment lignocellulose according to claim 1 and simultaneous saccharification and fermentation produce the method for hydrogen; It is characterized in that the hydrogenogens in the seed liquor of hydrogenogens in the step 2 is pyrolysis sugar anaerobic spore-bearing bacilli W16 (Thermoanaerobacterium thermosaccharolyticum W 16), disclose in the article that is called " Dark fermentation of xylose and glucose mix using isolated Thermoanaerobacterium thermosaccharolyticum W16 " that Thermoanaerobacterium thermosaccharolyticum W16 publish in " the International Journal of Hydrogen Energy " of 2008 the 33rd phase 6124-6132 pages or leaves.
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| CN106635887A (en) * | 2016-11-21 | 2017-05-10 | 华南理工大学 | Thermoanaerobacterium thermosaccharolyticum and application thereof to biological hydrogen production |
| CN108531515A (en) * | 2018-04-27 | 2018-09-14 | 哈尔滨工业大学 | A kind of method of fungi preprocessing lignocellulose and direct microorganism conversion fermentation and hydrogen production |
| CN109355315A (en) * | 2018-11-19 | 2019-02-19 | 华南理工大学 | The method and application of ethyl alcohol and succinic acid are produced using bagasse as raw material synchronous fermentation |
| CN109362813A (en) * | 2018-11-05 | 2019-02-22 | 贵州好菇粮农业科技有限公司 | A kind of mushroom growth regulator and preparation method thereof |
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| CN112852683A (en) * | 2021-03-24 | 2021-05-28 | 华南理工大学 | Thermosaccharophilus anaerobacter and application thereof |
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| CN106609294A (en) * | 2015-10-22 | 2017-05-03 | 中国科学院过程工程研究所 | Method of intensifying hydrogen production by double-bacterium fermented cellulose |
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| CN106635887B (en) * | 2016-11-21 | 2019-10-18 | 华南理工大学 | A kind of thermophilic solution sugar anaerobic bacillus(cillus anaerobicus) and its application in biological hydrogen production |
| CN106554974A (en) * | 2016-11-28 | 2017-04-05 | 东北大学 | A kind of method by the use of modified peanut as substrate fermentation hydrogen manufacturing is supplemented |
| CN108531515A (en) * | 2018-04-27 | 2018-09-14 | 哈尔滨工业大学 | A kind of method of fungi preprocessing lignocellulose and direct microorganism conversion fermentation and hydrogen production |
| CN109362813A (en) * | 2018-11-05 | 2019-02-22 | 贵州好菇粮农业科技有限公司 | A kind of mushroom growth regulator and preparation method thereof |
| CN109355315A (en) * | 2018-11-19 | 2019-02-19 | 华南理工大学 | The method and application of ethyl alcohol and succinic acid are produced using bagasse as raw material synchronous fermentation |
| CN110484571A (en) * | 2019-09-29 | 2019-11-22 | 哈尔滨工业大学 | Utilize the method for corn stover semi-successive cultivation hydrogen and grease |
| CN110484571B (en) * | 2019-09-29 | 2023-04-25 | 哈尔滨工业大学 | Method for semi-continuously producing hydrogen and grease by using corn straw |
| CN112852683A (en) * | 2021-03-24 | 2021-05-28 | 华南理工大学 | Thermosaccharophilus anaerobacter and application thereof |
| CN117185270A (en) * | 2023-09-05 | 2023-12-08 | 同济大学 | Method for recycling phosphorus from garden garbage |
| CN117185270B (en) * | 2023-09-05 | 2024-10-15 | 同济大学 | Method for recycling phosphorus from garden garbage |
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