CN106554974A - A kind of method by the use of modified peanut as substrate fermentation hydrogen manufacturing is supplemented - Google Patents
A kind of method by the use of modified peanut as substrate fermentation hydrogen manufacturing is supplemented Download PDFInfo
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- CN106554974A CN106554974A CN201611062715.5A CN201611062715A CN106554974A CN 106554974 A CN106554974 A CN 106554974A CN 201611062715 A CN201611062715 A CN 201611062715A CN 106554974 A CN106554974 A CN 106554974A
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- arachidis hypogaeae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
Abstract
The invention belongs to bio-hydrogen production technology field, more particularly to a kind of method by the use of modified peanut as substrate fermentation hydrogen manufacturing is supplemented.The present invention carries out pulverization process to Pericarppium arachidis hypogaeae first, and selectivity carries out pretreatment, configuration glucose fermentation produces hydrogen culture medium, pretreated Pericarppium arachidis hypogaeae is added to obtain anaerobic fermentation culture medium, it is seeded in the anaerobic fermentation culture medium containing modified peanut after the activation of hydrogenogen kind, at 30 35 DEG C, anaerobic hydrogen-generating is carried out under the conditions of 120 180rpm and is fermented 24 days, the biogas including hydrogen for producing are collected using drainage.Present invention uses substrate of the organic waste Pericarppium arachidis hypogaeae as fermentation and hydrogen production, reduces the cost of fermentation and hydrogen production, improve hydrogen generation efficiency, Pericarppium arachidis hypogaeae waste resource recycling is realized, the environmental effect brought because Pericarppium arachidis hypogaeae is burned is reduced.
Description
Technical field
The invention belongs to bio-hydrogen production technology field, more particularly to a kind of by the use of modified peanut as supplementary substrate fermentation
The method of hydrogen manufacturing.
Background technology
The problems such as life-time service of fossil energy will certainly cause lack of energy and air environmental pollution, therefore develop one
Plant environmentally friendly sustainable new forms of energy imperative.Hydrogen is cleaned as one kind, efficient, reproducible new forms of energy,
Increasingly paid attention to both at home and abroad.Compared with the hydrogen manufacturing modes such as methane cracking method, water gas conversion method, water electrolysis method, fermentation method
Biological hydrogen production has energy consumption low, renewable, pollution-free and using advantages such as cheap substrate.At present both at home and abroad to biological hydrogen production
Research is in the laboratory research stage, it is impossible to realize industrialization, industrialized development, the species of substrate, property and its can be sharp
The efficiency and operating cost for producing hydrogen is directly affected with rate, is to limit one of industrialized bottleneck sex chromosome mosaicism of biological hydrogen production.Therefore, it is honest and clean
Valency, resource, innoxious substrate are one of emphasis of research.
At present, the research of cheap substrates is mostly focused on changing food waste, industrial wastewater, stalk fibrid element bio-matrix
Deng both can realizing the resource of garbage, the production of clean energy resource can be realized simultaneously again.Pericarppium arachidis hypogaeae adds as a kind of agricultural product
Work residual fiber element class garbage, mostly as the direct burning disposal of rubbish, or as the adjuvant of fermented feed, causes greatly
The wasting of resources while, also bring certain environmental problem.And Pericarppium arachidis hypogaeae is similar to straw composition, containing substantial amounts of fibre
The natural high molecular substances such as dimension element, hemicellulose, lignin.Therefore, Pericarppium arachidis hypogaeae is had as the substrate of ferment for hydrogen production stronger
Operability, realize its resource, mass-energy, reached the purpose of clean manufacturing.
But the cellulose series biomass such as Pericarppium arachidis hypogaeae, straw, Caulis et Folium Oryzae are because of its complicated constituent and structure, it is difficult to by micro- life
Thing is directly utilized, and in order to accelerate the process of lignocellulose-like biomass bioenergy, needs to carry out raw material certain pre-
Process.Common preprocess method is divided into Physical（Such as crushing, high-energy ray etc.）, chemical method（Acid, alkali, oxidation processes etc.）、
Combination method（Acidifying steam explosion method, CO2Blasting procedure etc.）And bioanalysises（Whiterot fungi, brown rot fungus etc.）.Saha is equal to 2005 in temperature
140 DEG C, under conditions of sulfuric acid concentration is 0.75%, after processing wheat straw 30-60 min, can effectively improve follow-up enzymatic saccharification
Effect, monosaccharide yield is up to 55%；Dias etc. is in 2010 in research basidiomycetes Euc-1（Basidiomycetes Euc-1）With
Irpex lacteus（Irpexlacteus）Find when processing rice straw, in rice straw, cellulose and lignin ratio are by 2.7
Rise to 5.9 and 4.6.
Have much by the research that substrate carries out fermentation and hydrogen production of the fiber-like such as straw bio-matrix at present, but with Pericarppium arachidis hypogaeae and
There is not been reported as the substrate of fermentation and hydrogen production for modified peanut.China is used as peanut production big country, about 4,500,000 tons of annual output
Pericarppium arachidis hypogaeae, Pericarppium arachidis hypogaeae waste more serious as a kind of lignocellulose.
The content of the invention
For problem above, the present invention provides a kind of method by the use of modified peanut as substrate fermentation hydrogen manufacturing is supplemented,
Purpose is that the clean manufacturing of hydrogen is realized while using Semen arachidis hypogaeae shell resource, raising Pericarppium arachidis hypogaeae surcharge.
Realize that the technical scheme of the object of the invention is followed the steps below：
（1）Pericarppium arachidis hypogaeae pretreatment：Carry out to Pericarppium arachidis hypogaeae that pulverization process is standby, or ultrasound wave to be carried out to the Pericarppium arachidis hypogaeae after crushing pre-
Process or thermokalite method pretreatment；
（2）Configuration anaerobic fermentation culture medium：First configure glucose fermentation and produce hydrogen culture medium, then according to sending out per 50 milliliters of glucoses
Ferment adds the ratio of Pericarppium arachidis hypogaeae 0.2-1g to add pretreated Pericarppium arachidis hypogaeae in producing hydrogen culture medium, obtain anaerobic fermentation culture medium；
（3）Hydrogenogen kind is activated：By hydrogenogen kind under the conditions of 35 DEG C, 120 rpm, cultivate 2-3 days in activation medium, enter
Row activation；
（4）Fermentation and hydrogen production：In step（2）Anaerobic fermentation inoculation of medium activation after hydrogenogen kind, at 30-35 DEG C,
Carry out anaerobic hydrogen-generating to ferment 2-4 days under the conditions of 120-180rpm, the biogas including hydrogen for producing are collected using drainage.
Wherein, described pulverization process is that Pericarppium arachidis hypogaeae Jing clear water is washed away surface dirt, is placed in 60 DEG C of drying in oven 18-
24h, after granularity 0.2-0.5mm is milled to by plant pulverizer.
Described ultrasonic pretreatment is that the Pericarppium arachidis hypogaeae after crushing is added in centrifuge tube, is subsequently adding distilled water, Semen arachidis hypogaeae
Shell is 1 with the mass ratio of distilled water:10, being placed in ultrasonic cleaner carries out supersound process, dry for standby.
Described thermokalite method pretreatment is that Pericarppium arachidis hypogaeae submerges in the NaOH solution that mass concentration is 10%, is held at 70 DEG C
After 3 hours of continuous immersion, rinsed after being neutralized in being added to dilute hydrochloric acid solution repeatedly, dry for standby.
It is PYG culture medium that described glucose fermentation produces hydrogen culture medium, into being grouped into：Glucose 10g/L, peptone 4
G/L, yeast powder 1 g/L, KH2PO4 3.6 g/L、MgCl2•6H2O 0.2 g/L、FeSO4•7H2O 0.1 g/L、L-cysteine
0.5g/L。
Described activation medium is xylose media, into being grouped into：Xylose 10g/L, 4 g/L of peptone, yeast powder 1
g/L、KH2PO4 3.6g/L、MgCl2•6H2O 0.2 g/L、FeSO4•7H2O 0.1 g/L、L-cysteine 0.5g/L。
Compared with prior art, the characteristics of of the invention and beneficial effect are：
（1）Present invention uses substrate of the organic waste Pericarppium arachidis hypogaeae as fermentation and hydrogen production, realizes Pericarppium arachidis hypogaeae waste resource again
Utilize；
（2）The invention provides a kind of enrich the substrate of fermentation and hydrogen production by the use of Pericarppium arachidis hypogaeae as the hydrogen production process for supplementing substrate,
The usage amount of the conventional carbon sources such as glucose is reduced, this reduces the cost of fermentation and hydrogen production to a certain extent；
（3）The present invention carries out physics and chemical modification pretreatment to supplementing substrate Pericarppium arachidis hypogaeae, increases the surface area of Pericarppium arachidis hypogaeae, reduces
The degree of crystallinity of cellulose, improves the utilization rate of Pericarppium arachidis hypogaeae, improves hydrogen generation efficiency；
（4）The invention provides a kind of new way of Pericarppium arachidis hypogaeae resource, reduces the environment shadow brought because Pericarppium arachidis hypogaeae is burned
Ring.
Description of the drawings
Fig. 1 is the batch-type hydrogen production bioreactor figure that the embodiment of the present invention 1 ~ 3 is adopted；
Wherein：1：Simple anaerobic hydrogen-generating generator；2：Stirring in water bath device；3：Diving heating rod；4：Draining gas collecting apparatus；
Fig. 2 is the continuous hydrogen production bioreactor figure that the embodiment of the present invention 4 is adopted；
Wherein：5：Water outlet；6：Water inlet；7：Recirculated water；8：Thermometer；9：CSTR reactors（Full mixed anaerobic reaction device）；10：Stir
Mix device；11：Thermostat water bath.
Specific embodiment
The following examples can make those skilled in the art that the present invention is more completely understood, but limit never in any form
The present invention.
In the embodiment of the present invention, involved Pericarppium arachidis hypogaeae is the Pericarppium arachidis hypogaeae that market buys that Jing manually shells.
In the embodiment of the present invention 1 ~ 4, involved hydrogenogen kind ZGM211 is Guangxi clostridium ZGM211T
（Clostridium guangxiense ZGM211T）, the type strain of the strain was deposited in Chinese work on 2 1st, 2016
Industry Microbiological Culture Collection administrative center（CICC）, deposit number：CICC No. 24070.
Embodiment 1
The method by the use of crushing modified peanut as substrate fermentation hydrogen manufacturing is supplemented of the present embodiment, using the device shown in Fig. 1,
Follow the steps below：
（1）Pericarppium arachidis hypogaeae pretreatment：Pulverization process is carried out to Pericarppium arachidis hypogaeae, Pericarppium arachidis hypogaeae Jing clear water is washed away into surface dirt, 60 DEG C are placed in
Drying in oven 24h, after granularity 0.2-0.5mm is milled to by plant pulverizer；
（2）Configuration anaerobic fermentation culture medium：First configure glucose fermentation and produce hydrogen culture medium, then according to sending out per 50 milliliters of glucoses
Ferment adds the ratio of crushing peanuts shell 1g to add the Pericarppium arachidis hypogaeae after comminution pretreatment in producing hydrogen culture medium, obtain anaerobic fermentation culture
Base, it is PYG culture medium that described glucose fermentation produces hydrogen culture medium, into being grouped into：Glucose 10g/L, 4 g/L of peptone,
Yeast powder 1 g/L, KH2PO4 3.6 g/L、MgCl2•6H2O 0.2 g/L、FeSO4•7H2O 0.1 g/L、L-cysteine
0.5g/L；
（3）Hydrogenogen kind is activated：By hydrogenogen kind ZGM211 under the conditions of 35 DEG C, 120 rpm, 2- is cultivated in activation medium
3 days, activated, described activation medium is xylose media, into being grouped into：Xylose 10g/L, 4 g/L of peptone, ferment
Female powder 1 g/L, KH2PO4 3.6g/L、MgCl2•6H2O 0.2 g/L、FeSO4•7H2O 0.1 g/L、L-cysteine 0.5g/
L；
（4）Fermentation and hydrogen production：Above-mentioned anaerobic fermentation culture medium 50mL is added in 100mL cillin bottles, is added in separately taking 100mL cillin bottles
Enter the PYG culture fluid 50mL for having configured as a comparison, oxygen is discharged using high pure nitrogen stripping, and after sterilizing, using aseptic
Syringe accesses ZGM211 bacterium solutions 1mL in activation culture 2-3 days, the concussion and cultivate in 35 DEG C, 120rpm shaking tables；Using Fig. 1
Shown draining gas collecting apparatus, collects the biogas that hydrogenogen kind is produced with drainage, using Agilent 7890A gas chromatograies
Instrument is analyzed to biogas composition and content, and the maximum hydrogen output for calculating PYG culture medium is 46.4 mL, adds and supplements bottom
After thing, maximum hydrogen output is improved to 52mL, and its hydrogen yield is up to 5.6mL-H2/g-peanut shell。
Embodiment 2
The method by the use of crushing modified peanut as substrate fermentation hydrogen manufacturing is supplemented of the present embodiment, using dress as shown in Figure 1
Put, follow the steps below：
（1）Pericarppium arachidis hypogaeae pretreatment：Pulverization process is carried out to Pericarppium arachidis hypogaeae, Pericarppium arachidis hypogaeae Jing clear water is washed away into surface dirt, 60 DEG C are placed in
Drying in oven 18h, after granularity 0.2-0.5mm is milled to by plant pulverizer；
（2）Configuration anaerobic fermentation culture medium：First configure glucose fermentation and produce hydrogen culture medium, according to glucose and Pericarppium arachidis hypogaeae（mL/
mg）Proportioning parameter 1:2、1:4、1:6、1:8、1:10, the Pericarppium arachidis hypogaeae sample of pulverization process is added, anaerobic fermentation culture is obtained
Base, it is PYG culture medium that described glucose fermentation produces hydrogen culture medium, into being grouped into：Glucose 10g/L, 4 g/L of peptone,
Yeast powder 1 g/L, KH2PO4 3.6 g/L、MgCl2•6H2O 0.2 g/L、FeSO4•7H2O 0.1 g/L、L-cysteine
0.5g/L；
（3）Hydrogenogen kind is activated：By hydrogenogen kind ZGM211 under the conditions of 35 DEG C, 120 rpm, 2- is cultivated in activation medium
3 days, activated, described activation medium is xylose media, into being grouped into：Xylose 10g/L, 4 g/L of peptone, ferment
Female powder 1 g/L, KH2PO4 3.6g/L、MgCl2•6H2O 0.2 g/L、FeSO4•7H2O 0.1 g/L、L-cysteine 0.5g/
L；
（4）Fermentation and hydrogen production：The each 50mL of above-mentioned different ratio anaerobic fermentation culture medium is added in each 100mL cillin bottles, is separately taken
The PYG culture fluid 50mL for having configured is added in 100mL cillin bottles as a comparison, oxygen is discharged using high pure nitrogen stripping, and
After sterilizing, ZGM211 bacterium solutions 1mL in activation culture 2-3 days are accessed using asepsis injector, shake in 35 DEG C, 120rpm shaking tables
Swing culture；The biogas for producing are collected using gas collecting bottle, using Agilent 7890A gas chromatograpies are to biogas composition and contain
Amount is analyzed.Optimum proportioning is calculated for 1:10, maximum hydrogen output now is 75.2mL, and maximum hydrogen yield is
13.67mL-H2/ g- substrates, Pericarppium arachidis hypogaeae highest hydrogen yield is up to 5.76mL-H2/g- peanut shell。
Embodiment 3
The method by the use of supersonic modifying Pericarppium arachidis hypogaeae as substrate fermentation hydrogen manufacturing is supplemented of the present embodiment, using dress as shown in Figure 1
Put, follow the steps below：
（1）Pericarppium arachidis hypogaeae pretreatment：Pulverization process is carried out to Pericarppium arachidis hypogaeae, Pericarppium arachidis hypogaeae Jing clear water is washed away into surface dirt, 60 DEG C are placed in
Drying in oven 22h, after granularity 0.2-0.5mm is milled to by plant pulverizer, ultrasound is carried out to the Pericarppium arachidis hypogaeae after crushing then
Ripple pretreatment, described ultrasonic pretreatment are that the Pericarppium arachidis hypogaeae after crushing is added in centrifuge tube, are subsequently adding distilled water, Semen arachidis hypogaeae
Shell is 1 with the mass ratio of distilled water:10, being placed in ultrasonic cleaner carries out supersound process, ultrasonic pretreatment parameter such as 1 institute of table
Show, 16 pretreated groups and 1 blank control group, dry for standby are set altogether.
（2）Configuration anaerobic fermentation culture medium：First configure glucose fermentation and produce hydrogen culture medium, then according to sending out per 50 milliliters of glucoses
Ferment adds the ratio of ultrasonic Pericarppium arachidis hypogaeae 1g to add ultrasonic pretreated Pericarppium arachidis hypogaeae in producing hydrogen culture medium, obtain anaerobic fermentation culture
Base, it is PYG culture medium that described glucose fermentation produces hydrogen culture medium, into being grouped into：Glucose 10g/L, 4 g/L of peptone,
Yeast powder 1 g/L, KH2PO4 3.6 g/L、MgCl2•6H2O 0.2 g/L、FeSO4•7H2O 0.1 g/L、L-cysteine
0.5g/L；
（3）Hydrogenogen kind is activated：By hydrogenogen kind ZGM211 under the conditions of 35 DEG C, 120 rpm, 2- is cultivated in activation medium
3 days, activated, described activation medium is xylose media, into being grouped into：Xylose 10g/L, 4 g/L of peptone, ferment
Female powder 1 g/L, KH2PO4 3.6g/L、MgCl2•6H2O 0.2 g/L、FeSO4•7H2O 0.1 g/L、L-cysteine 0.5g/
L；
（4）Fermentation and hydrogen production：Above-mentioned anaerobic fermentation culture medium 50mL is added in 100mL cillin bottles, is added in separately taking 100mL cillin bottles
Enter the PYG culture fluid 50mL for having configured as a comparison, oxygen is discharged using high pure nitrogen stripping, and after sterilizing, using aseptic
Syringe accesses ZGM211 bacterium solutions 1mL in activation culture 2-3 days, the concussion and cultivate in 35 DEG C, 120rpm shaking tables；Use
Agilent 7890A gas chromatograpies are analyzed to biogas composition and content.Calculate the maximum hydrogen output of PYG culture fluid
For 46.4 mL, maximum hydrogen output after supplementing substrate is added to improve to 61.7mL its hydrogen yield up to 15.3mL-H2/g-
Peanut shell, optimal ultrasound condition are 400W-20 min.
Embodiment 4
The method by the use of thermokalite method modified peanut as substrate fermentation hydrogen manufacturing is supplemented of the present embodiment, using the dress described in Fig. 2
Put, follow the steps below：
（1）Pericarppium arachidis hypogaeae pretreatment：Pulverization process is carried out to Pericarppium arachidis hypogaeae, Pericarppium arachidis hypogaeae Jing clear water is washed away into surface dirt, 60 DEG C are placed in
Drying in oven 18h, after granularity 0.2-0.5mm is milled to by plant pulverizer, thermokalite is carried out to the Pericarppium arachidis hypogaeae after crushing then
Method pretreatment, described thermokalite method pretreatment are that Pericarppium arachidis hypogaeae submerges in the NaOH solution that mass concentration is 10%, are held at 70 DEG C
After 3 hours of continuous immersion, rinsed after being neutralized in being added to dilute hydrochloric acid solution repeatedly, dry for standby；
（2）Configuration anaerobic fermentation culture medium：First configure glucose fermentation and produce hydrogen culture medium, according to glucose and Pericarppium arachidis hypogaeae（mL/
mg）Proportioning parameter 1:2、1:4、1:6、1:8、1:10, the Pericarppium arachidis hypogaeae sample for adding thermokalite method to process obtains anaerobic fermentation culture
Base, it is PYG culture medium that described glucose fermentation produces hydrogen culture medium, into being grouped into：Glucose 10g/L, 4 g/L of peptone,
Yeast powder 1 g/L, KH2PO4 3.6 g/L、MgCl2•6H2O 0.2 g/L、FeSO4•7H2O 0.1 g/L、L-cysteine
0.5g/L；
（3）Hydrogenogen kind is activated：By hydrogenogen kind ZGM211 under the conditions of 35 DEG C, 120 rpm, 2- is cultivated in activation medium
3 days, activated, described activation medium is xylose media, into being grouped into：Xylose 10g/L, 4 g/L of peptone, ferment
Female powder 1 g/L, KH2PO4 3.6g/L、MgCl2•6H2O 0.2 g/L、FeSO4•7H2O 0.1 g/L、L-cysteine 0.5g/
L；
（4）Fermentation and hydrogen production：The each 50mL of above-mentioned different ratio anaerobic fermentation culture medium is added in each 100mL cillin bottles, is separately taken
The PYG culture fluid 50mL for having configured is added in 100mL cillin bottles as a comparison, oxygen is discharged using high pure nitrogen stripping, and
After sterilizing, ZGM211 bacterium solutions 1mL in activation culture 2-3 days are accessed using asepsis injector, shake in 35 DEG C, 120rpm shaking tables
Swing culture；The biogas for producing are collected using drainage, using Agilent 7890A gas chromatograpies are to biogas composition and contain
Amount is analyzed.Optimum proportioning is calculated for 1:10, maximum hydrogen output now is 96.2mL, and maximum hydrogen yield is
17.49mL-H2/ g- substrates, Pericarppium arachidis hypogaeae hydrogen yield is up to 9.96mL-H2/g-peanut shell。
Embodiment 5
The present embodiment is to carry out continuous fermentation hydrogen manufacturing by the use of crushing peanuts shell as substrate is supplemented, and is comprised the following steps that：
（1）Using being made up of lucite, dischargeable capacity is the continuous stream stirred-tank reactor of 5L（CSTR）, inside set solution-air-
Gu three-phase separating device, with 1:The Pericarppium arachidis hypogaeae of the Mel waste water and pulverization process of 10 proportionings is substrate, and adds a small amount of carbamide, phosphorus
Acid dihydride potassium causes COD:N:P=500:5:1；
（2）Plant mud and take from and carry out Shenyang City Liaozhong County secondary sedimentation tank of sewage treatment work, Jing after filtering, precipitate, eluriate, exposed with molasses containing waste water
Air culture is seeded in reactor after supporting 7d, and sludge seeding amount MLVSS is 8.52g/L, is 8h, water inlet in 35 ± 1 DEG C of temperature, HRT
It is to start CSTR under the conditions of 7 etc. that COD concentration is 2000 mg/L, inlet flow-patterm；
（3）The parameters such as pH, ORP, temperature, HRT are adjusted progressively, after the operation of question response device reaches steady statue, using gas collection
Bottle collects the biogas for producing, and biogas composition and content is analyzed using Agilent 7890A gas chromatograpies；
（4）System hydrogen-producing speed under relatively low OLR is larger, and during operation 20d, system reaches steady statue, calculates sludge highest
Hydrogen generation efficiency up to 8.6mmol/L/h, ethanol and acetic acid content account for total terminal aqueous production mass ratio be 83.4%
Illustrate that technical scheme is applicable to continuous industry production.
Claims (6)
1. a kind of method by the use of modified peanut as substrate fermentation hydrogen manufacturing is supplemented, it is characterised in that enter according to following steps
OK：
（1）Pericarppium arachidis hypogaeae pretreatment：Carry out to Pericarppium arachidis hypogaeae that pulverization process is standby, or ultrasound wave to be carried out to the Pericarppium arachidis hypogaeae after crushing pre-
Process or thermokalite method pretreatment；
（2）Configuration anaerobic fermentation culture medium：First configure glucose fermentation and produce hydrogen culture medium, then according to sending out per 50 milliliters of glucoses
Ferment adds the ratio of Pericarppium arachidis hypogaeae 0.2-1g to add pretreated Pericarppium arachidis hypogaeae in producing hydrogen culture medium, obtain anaerobic fermentation culture medium；
（3）Hydrogenogen kind is activated：By hydrogenogen kind under the conditions of 35 DEG C, 120 rpm, cultivate 2-3 days in activation medium, enter
Row activation；
（4）Fermentation and hydrogen production：In step（2）Anaerobic fermentation inoculation of medium activation after hydrogenogen kind, at 30-35 DEG C,
Carry out anaerobic hydrogen-generating to ferment 2-4 days under the conditions of 120-180rpm, the biogas including hydrogen for producing are collected using drainage.
2. a kind of method by the use of modified peanut as substrate fermentation hydrogen manufacturing is supplemented according to claim 1, its feature
Be described pulverization process be that Pericarppium arachidis hypogaeae Jing clear water is washed away into surface dirt, be placed in 60 DEG C of drying in oven 18-24h, after by
Plant pulverizer is milled to granularity 0.2-0.5mm.
3. a kind of method by the use of modified peanut as substrate fermentation hydrogen manufacturing is supplemented according to claim 1, its feature
Be described ultrasonic pretreatment be that the Pericarppium arachidis hypogaeae after crushing is added in centrifuge tube, be subsequently adding distilled water, Pericarppium arachidis hypogaeae with
The mass ratio of distilled water is 1:10, being placed in ultrasonic cleaner carries out supersound process, dry for standby.
4. a kind of method by the use of modified peanut as substrate fermentation hydrogen manufacturing is supplemented according to claim 1, its feature
Thermokalite method pretreatment described in being is that crushing peanuts shell submerges in the NaOH solution that mass concentration is 10%, is held at 70 DEG C
After 3 hours of continuous immersion, rinsed after being neutralized in being added to dilute hydrochloric acid solution repeatedly, dry for standby.
5. a kind of method by the use of modified peanut as substrate fermentation hydrogen manufacturing is supplemented according to claim 1, its feature
It is that to produce hydrogen culture medium be PYG culture medium to described glucose fermentation, into being grouped into：Glucose 10g/L, 4 g/L of peptone,
Yeast powder 1 g/L, KH2PO4 3.6 g/L、MgCl2•6H2O 0.2 g/L、FeSO4•7H2O 0.1 g/L、L-cysteine
0.5g/L。
6. a kind of method by the use of modified peanut as substrate fermentation hydrogen manufacturing is supplemented according to claim 1, its feature
Be described activation medium be xylose media, into being grouped into：Xylose 10g/L, 4 g/L of peptone, 1 g/ of yeast powder
L、KH2PO4 3.6g/L、MgCl2•6H2O 0.2 g/L、FeSO4•7H2O 0.1 g/L、L-cysteine 0.5g/L。
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| CN102031276A (en) * | 2009-09-28 | 2011-04-27 | 中国科学院过程工程研究所 | Method for producing hydrogen by fermenting organism and alkali mat composite pretreated straw fiber raw erial at two steps |
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| CN102321671A (en) * | 2011-09-19 | 2012-01-18 | 哈尔滨工业大学 | Method for biologic pretreatment of lignocellulose and hydrogen production through simultaneous saccharification and fermentation |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116287014A (en) * | 2023-02-16 | 2023-06-23 | 中国矿业大学 | Preparation method and application of fermentation substrate for hydrogen production by sulfate reducing bacteria |
| CN116287014B (en) * | 2023-02-16 | 2023-12-01 | 中国矿业大学 | Preparation method and application of fermentation substrate for hydrogen production by sulfate reducing bacteria |
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