CN116287014B - Preparation method and application of fermentation substrate for hydrogen production by sulfate reducing bacteria - Google Patents
Preparation method and application of fermentation substrate for hydrogen production by sulfate reducing bacteria Download PDFInfo
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/22—Klebsiella
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Abstract
The invention provides a preparation method and application of a fermentation substrate for preparing hydrogen by sulfate reducing bacteria, and belongs to the technical field of biological hydrogen production. Treating a substrate, and firstly crushing the crushed powder with the granularity of less than 5 mm; mixing the substrate fines with an organic solvent; ultrasonic extraction is carried out on the mixture of the organic solvent and the substrate for 2 hours under the power of 350W, the extraction of the organic solvent damages the physical morphology of the substrate fragments, and partial inorganic micromolecules are extracted, so that the pores of the substrate fragments become larger and the structure is more loose; filtering and separating the organic solvent liquid and the substrate, volatilizing residual organic solvent from the substrate after the heating and drying treatment to homogenize the particle size distribution of the substrate fragments, and utilizing the substrate fragments by exogenous bacteria more easily, wherein the final product is a fermentation substrate. The method activates the nondegradable lignite through pretreatment of the organic solvent, changes the physical properties of the lignite, and can obviously improve the hydrogen production efficiency; the lignite is recycled and utilized cleanly.
Description
Technical Field
The invention relates to a preparation method and application of a fermentation substrate for preparing hydrogen by sulfate reducing bacteria, and belongs to the technical field of biological hydrogen production.
Background
Hydrogen fuel has a high unit energy content (2.75 times higher than hydrocarbon fuel) and does not generate greenhouse gases when burned, and is therefore considered as an ideal energy carrier in the future. However, the technology of producing hydrogen still relies on energy intensive processes, which are not sustainable. Thus, there is an urgent need for a process for producing hydrogen in a renewable, sustainable and environmentally friendly manner. Biological hydrogen production is oneA very effective alternative is because it requires a low energy input and is sustainable if waste or renewable substrates are used. Coal is a widely used resource, but a large part of it exists in the form of brown coal of poor quality. It is very important to break through and develop key technologies for bioconversion of coal. Whereas sulfate-reducing bacteria are a major class of environmental anaerobic bacteria characterized by very high levels of hydrogenase responsible for the production or consumption of H 2 Often there are multiple copies in the genome, so sulfate-reducing bacteria have a high potential for hydrogen production.
In the prior art: the invention patent number 201410309486.7 relates to a strain for producing hydrogen by fermenting xylose and a hydrogen production method. The strain is bacillus cereus S1 strain and Brevundimonas slimichiganensis Z1 strain, is obtained based on xylose degradation screening, and has higher hydrogen production capacity. The hydrogen production method takes xylose as a substrate, adopts bacillus cereus S1 strain and/or Brevundimonas slimichizome Z1 strain to ferment and cultivate to produce hydrogen, needs to use xylose as the substrate, has high cost, and is not suitable for large-scale application in industry.
The patent numbers are as follows: 201710173666.0A method for pretreating peanut shells by combining ultralow temperature and crushing is disclosed, wherein the dried peanut shells are placed in an ultralow temperature environment for a period of time, and are taken out and crushed in a physical way rapidly; placing the treated peanut shells and a glucose anaerobic fermentation culture medium into a culture flask, and sterilizing at high temperature after filling nitrogen; and inoculating the activated anaerobic hydrogen-producing strain into the mixed glucose anaerobic fermentation culture medium, and placing the mixed glucose anaerobic fermentation culture medium under a proper condition to perform anaerobic biological fermentation to produce hydrogen. The method has severe requirements on the environmental temperature, so that the whole scheme is severely limited by the environmental conditions, and the production cost is obviously increased by manually simulating the low-temperature environment, so that the method is not suitable for industrial large-scale production.
Disclosure of Invention
Aiming at the defects of the prior art, the preparation method and the application of the fermentation substrate for preparing the hydrogen by using the sulfate reducing bacteria are provided, the lignite is used as a substrate, the organic solvent is firstly used for activating the lignite to increase the bioavailability of the lignite, then the sulfate reducing bacteria are used for carrying out biological fermentation to produce hydrogen, the physical and chemical properties of the substrate after the hydrogen production are not obviously changed, the substrate can be continuously used, and the preparation method and the application of the fermentation substrate have the advantages of environmental protection, high stability, short period, high hydrogen production efficiency and the like. Has good economic benefit and is beneficial to realizing commercial application.
In order to achieve the technical aim, the preparation method of the fermentation substrate for producing hydrogen by using the sulfate reducing bacteria comprises the following steps:
treating a substrate, and firstly crushing the crushed powder with the granularity of less than 5 mm;
mixing the substrate fines with an organic solvent;
ultrasonic extraction is carried out on the mixture of the organic solvent and the substrate for 2 hours under the power of 350W, the extraction of the organic solvent damages the physical morphology of the substrate fragments, and partial inorganic micromolecules are extracted, so that the pores of the substrate fragments become larger and the structure is more loose;
filtering and separating the organic solvent liquid and the substrate, volatilizing residual organic solvent from the substrate after the heating and drying treatment to homogenize the particle size distribution of the substrate fragments, and utilizing the substrate fragments by exogenous bacteria more easily, wherein the final product is a fermentation substrate.
Further, the organic solvent is one of alcohol, ester, hydrocarbon and ether, and 1L of organic solvent is added to 100g of coal dust.
Further, the substrate is pre-treated lignite powder.
A fermentation medium prepared by a fermentation medium matrix preparation method for preparing hydrogen by using sulfate reducing bacteria.
Use of a fermentation substrate characterized in that: mixing the pretreated lignite powder with activated sulfate reducing bacteria seed liquid, and carrying out anaerobic fermentation in a fermentation medium to prepare hydrogen.
Further, the strain in the seed liquid of the sulfate reducing bacteria is Klebsiella aerogenes Klebsiella aerogenes, the strain is Brevibacterium, the strain utilizes sulfate as a respiratory chain terminal electron acceptor to reduce the sulfate into sulfide, logarithmic growth log phase is carried out for 10-36 hours, the optimal growth pH is 7.0, the optimal temperature is 35 ℃, and the strain is commercially available.
Further, the activated sulfate reducing bacteria seed solution consists of sulfate reducing bacteria and a seed culture medium, wherein the seed culture medium comprises the following components in percentage by mass: dipotassium hydrogen phosphate 0.005%, yeast extract 0.01%, sodium sulfate 0.005%, magnesium sulfate heptahydrate 0.02%, cysteine hydrochloride 0.01%, sodium lactate 0.07% at 50%, ammonium chloride 0.01%, calcium chloride dihydrate 0.01%, resazurin 0.01% at 0.1% and water 99.85%; glucose, buffer solution, trace elements and components beneficial to bacterial production are added into the culture medium according to the requirement, and the culture process of the bacteria is also suitable for.
Further, a fermentation medium used for anaerobic fermentation comprises pretreated lignite powder, basic inorganic salt and water; wherein the basic inorganic salt of the fermentation medium comprises the following components in percentage by mass: dipotassium hydrogen phosphate 0.005%, sodium sulfate 0.005%, magnesium sulfate heptahydrate 0.02%, ammonium chloride 0.01%, calcium chloride dihydrate 0.01%, substrate 10% and water 89.95%; wherein the substrate of the fermentation medium is organic matter sediment; 1.0g of cysteine hydrochloride is additionally added into each liter of the prepared fermentation medium to maintain the reducibility in the fermentation process.
Further, the fermentation temperature of the substrate in the fermentation culture is 35 ℃, and the anaerobic fermentation period is 7 days.
A method for producing hydrogen by anaerobic fermentation of hydrogen-producing microorganisms.
The generated hydrogen can be sucked from the anaerobic fermentation reactor by a disposable needle tube, and the content of the hydrogen is detected by adopting gas chromatography.
The beneficial effects are that:
firstly, the lignite is pretreated, the lignite is converted into a state which is easy to be converted and utilized by microorganisms, and sulfate reducing bacteria are further utilized for fermentation to produce hydrogen. Experiments prove that compared with raw coal, the efficiency of hydrogen production by fermentation of the pretreated lignite is obviously improved, and the efficiency of the lignite group pretreated by the alcohol organic solvent is most obvious and is twice that of the raw coal gas production group, and the efficiency reaches 19.13 mu mol/g coal. Lignite after gas production can also be normally used as other production activities. The invention realizes the clean utilization of the lignite resource.
The invention uses sulfate reducing bacteria to carry out anaerobic fermentation on lignite pretreated by organic solvent to generate hydrogen. Compared with the existing hydrogen production technology, the technology aims at the substrate which is a macromolecule (organic matter sediment) difficult to degrade; the organic matters which are difficult to degrade are activated through pretreatment of the organic solvent, so that the hydrogen production efficiency can be obviously improved; can realize the resource utilization of macromolecule degradation-resistant organic solid waste; the hydrogen production energy consumption can be obviously reduced by microbial fermentation for hydrogen production.
Drawings
FIG. 1 is an SEM photograph of four types of lignite before and after pretreatment, wherein a is raw coal, b is alcohol-type raffinate coal, c is ester-type raffinate coal, d is ether-type raffinate coal, and e is hydrocarbon-type raffinate coal;
FIG. 2 is an XRD pattern of raw coal and four types of organic solvent raffinate coal;
FIG. 3 is a graph showing the hydrogen production of different organic solvent treated coals.
Detailed Description
Embodiments of the invention are further described below with reference to the accompanying drawings:
the hydrogen-producing strain used in the invention is a sulfate reduction strain Klebsiella SKA1 separated from a coal biogas production system, the strain is Brevibacterium, the size of the strain is about (1.5-2.2) mu m x (0.4-0.8) mu m, the strain can reduce sulfate into sulfide by using sulfate as a respiratory chain terminal electron acceptor, the sulfate enters a growth logarithmic phase after 10-36 hours, the optimal growth pH is 7.0, and the optimal temperature is 35 ℃.
The invention provides a method for producing hydrogen, which comprises anaerobic fermentation by using hydrogen-producing microorganisms, wherein the hydrogen-producing microorganisms are sulfate reducing bacteria-Klebsiella aerogenes (Klebsiella aerogenes), and further preferably the Klebsiella aerogenes is in logarithmic growth phase as hydrogen-producing microorganisms.
The method for producing hydrogen provided by the invention comprises the following steps:
the scheme for preprocessing the substrate is as follows: firstly, crushing a substrate by using a crusher, crushing the substrate into powder with the granularity smaller than 5mm, mixing an organic solvent with the substrate, performing ultrasonic treatment, filtering to separate the treated organic solvent liquid from the substrate, and heating and drying the treated substrate to volatilize residual treatment liquid, thereby completing pretreatment.
Activating the hydrogen-producing microorganism, and then inoculating the hydrogen-producing microorganism into a seed culture medium for anaerobic culture to obtain seed liquid;
inoculating the seed liquid into a fermentation medium containing a pretreated substrate for anaerobic fermentation culture to obtain hydrogen.
In a specific embodiment, the substrate is pretreated lignite, shale, oil shale, tar sands, bitumen, peat, and similar organic-containing deposits. The pretreatment may include pulverization by a pulverizer (the substrate having a large particle size is subjected to the operation), treatment with an organic solvent, filtration to separate the treatment liquid from the substrate, and heating to volatilize the residual treatment liquid from the treated substrate.
In a specific embodiment, the processing step is comminution by a pulverizer. The substrate with larger particle size is crushed into powder with particle size smaller than 5 mm.
In a specific embodiment, the processing step is an organic solvent pretreatment.
In a specific embodiment, the organic solvent used for the organic solvent treatment is an organic solvent of the alcohol, ester, hydrocarbon, ether or the like type. Mixing the substrate powder with an organic solvent, performing ultrasonic extraction on the organic solvent and substrate mixture for 2 hours under the power of 350W, and adding 1L of the organic solvent into 100g of coal dust. The present method proposes four types of organic solvents, but is not limited to the four types of organic solvents proposed herein.
In a specific embodiment, the processing step is solid-liquid separation. Filtering and separating the solid-liquid mixture after the organic solvent treatment, wherein the treated substrate can be used as a substrate for anaerobic fermentation of hydrogen-producing microorganisms, and the mixed liquid of the treated organic solvent treatment can be collected and recycled for the next substrate treatment.
In a specific embodiment, the processing step is heating the treatment fluid remaining on the substrate after evaporation filtration. The filtered substrate was heated to 50 ℃ to volatilize the treatment fluid until the substrate was completely dried.
Wherein, in a specific embodiment, the seed culture medium consists of dipotassium hydrogen phosphate, yeast extract, sodium sulfate, magnesium sulfate, heptawater, cysteine hydrochloride, 50% concentration sodium lactate, ammonium chloride, calcium chloride, dihydrate, 0.1% concentration resazurin and water, and the mass percentages in the seed culture medium are respectively 0.005%, 0.01%, 0.005%, 0.02%, 0.01%, 0.07%, 0.01% and 99.85%. Anaerobic culture.
It is worth to say that the most common culture medium of the strain seed culture medium is only given in the description, and the components beneficial to bacterial production, such as glucose, buffer solution, other trace elements and the like, are added into the culture medium and are also suitable for the bacterial culture process.
Wherein, the basic inorganic salt of the fermentation culture medium consists of dipotassium hydrogen phosphate, sodium sulfate, magnesium sulfate, heptahydrate, ammonium chloride and calcium chloride, and the mass percentages of the basic inorganic salt are respectively 0.005%, 0.02%, 0.01% and 0.01%. Wherein the substrate of the fermentation medium is organic matter sediment, accounting for 10% of the whole fermentation medium. Water represents 89.95% of the whole fermentation medium. 1.0g of cysteine hydrochloride is additionally added into each liter of the prepared fermentation medium to maintain the reducibility in the fermentation process.
In a specific embodiment, the substrate is present in the anaerobic fermentation medium in an amount of 80 to 100g/L.
In the fermentation culture, the fermentation temperature is 35 ℃ and the fermentation time is 7 days.
Examples
EXAMPLE 1 isolation of species
The Klebsiella aerogenes (Klebsiella aerogenes) strain used in the experiment was isolated from a coal biogas production system.
After multiple roller tube separation and purification experiments, a single black colony appearing in an anaerobic test tube can be obtained, the single black colony is picked out to a liquid seed culture medium for expansion culture, and the obtained bacterial liquid is subjected to molecular biological assay. Uploading the sequencing result to NCBI comparison, searching strains with similar homology to the test strains, selecting 15 bacteria with higher homology, and drawing a phylogenetic tree of the strains. As a result, the test strain was found to have relatively close homology to Klebsiella aerogenes (Klebsiella aerogenes), and thus the strain obtained in this study was considered to belong to Proteus, klebsiella aerogenes. The strain belongs to common klebsiella, and is a commercially available strain.
EXAMPLE 2 pretreatment of substrates
The substrate selected by the invention is similar organic matter-containing sediments such as lignite, shale, oil shale, tar sands, asphalt, peat and the like. Because these substances are all hydrocarbon compounds and have similar chemical properties, they can be utilized by sulfate reducing bacteria to produce hydrogen. In this embodiment, lignite is taken as an example, and a lignite pretreatment flow is described.
Firstly, taking a coal sample of the lignite No. 5 of victory, grinding the coal sample by a grinder until the granularity is less than 5mm, then sub-packaging the coal sample, and uniformly mixing the coal sample with organic solvents of alcohol, ester, hydrocarbon and ether respectively, wherein each liter of organic solvent is required to contain 100g of lignite powder. And then placing the uniformly mixed lignite organic solvent mixed solution into an ultrasonic cleaner for ultrasonic extraction for 2 hours under the power of 350W. And then taking out the pulverized coal organic solvent mixture after ultrasonic extraction, standing and precipitating for 1 hour, and filtering to separate solid from liquid. The filtered coal powder is dried for 2 hours at 50 ℃ in an electrothermal blowing drying box. The dried coal powder can be used as a substrate of a fermentation medium. The filtered organic solvent can be collected for the next extraction of lignite powder.
Scanning Electron Microscope (SEM) observation is carried out on raw coal and pretreated coal samples, and the results are shown in the accompanying figure 1, a: raw coal, b: alcohol raffinate coal, c: ester raffinate coal, d: ether raffinate coal, e: hydrocarbon raffinate coal. Under the scanning electron microscope, the raw coal particles are non-uniform, but have a compact structure, wherein small particles are piled up or adhered in gaps among large particles, and the surface is relatively flat. After pretreatment, the particle size distribution becomes uniform gradually, and a clear gap appears between the particles. At the same time, it can be seen that the compact overall sheet structure in the raw coal becomes an open block and grain structure. It is known that the extraction effect can destroy the morphology of the coal, and can extract part of small molecules so that the pores become large. The Matlab analysis software is used for analyzing the porosity and the porosity ratio of raw coal and the pretreated coal sample, and the result shows that the porosity and the porosity ratio of the coal sample are increased to different degrees after pretreatment by four types of organic solvents, and the porosity of the coal is increased and the structure is looser after pretreatment by the organic solvents, so that the coal is easier to be utilized by exogenous bacteria.
The raw coal and the pretreated coal sample are subjected to X-ray diffraction (XRD) characterization, and the result is shown in figure 2. The result shows that diffraction characteristic peaks in the raffinate coal are increased or disappeared compared with the raw coal, but the characteristic peaks are smaller, the content of corresponding mineral matters is smaller, the extraction effect of the solvent is shown, so that the content of partial inorganic mineral matters in the coal sample is reduced, the quality is reduced, the intermolecular pores in the coal sample are increased, the coal sample is easier to be degraded and utilized by microorganisms, and the conclusion is mutually proved with the industrial analysis result of the coal sample, as shown in the table 1;
TABLE 1 results of porosity and void ratio of raw coal and four types of organic solvent pretreated coal samples
Coal is an amorphous material, but there is a small amount of graphite-like structure in the coal, so the method of researching graphite can be used for researching the coal. The 002 peak and the 100 peak in the XRD spectrum of the coal sample are taken as characteristic peaks for analysis and calculation, the 002 peak can reflect the stacking among the aromatic ring layers, and corresponds to the aromatic microcrystal formed by the polycondensation aromatic nucleus, and the 100 peak can reflect the condensation degree of the aromatic ring, namely the size of the aromatic carbon network sheet. Calculating microcrystalline structure parameters of the coal sample by using Bragg and Scherrer equations, wherein lambda is 0.15405nm, and the results are shown in Table 2;
TABLE 2 microcrystalline structure parameters for raw coal and four types of organic solvent raffinate coal
Among the four kinds of raffinate coals, the parameter La reflecting the extensibility is also quite different, the extensibility of the ester and hydrocarbon raffinate coals is smaller than that of the raw coals, and the extensibility of the alcohol and ether raffinate coals is larger than that of the raw coals, which is probably because the alcohols and ethers have different polarities and hydrophilicities with the esters and hydrocarbons, the penetrating power on the internal structure of the coals is different, the effect on the aromatic layers is also different, the microcrystalline structure of the coals has certain elasticity, and the difference of the swelling capacity of the organic solvents also leads to different changes of La. The N value fluctuates at about 2.5, which shows that the extraction effect of the organic solvent has no great influence on the average stacking layer number, and among four kinds of raffinate coals, the ester raffinate coals and the ether raffinate coals are larger than raw coals, and the alcohol and hydrocarbon raffinate coals are smaller than raw coals, so that the structure of the coal sample is unstable after the extraction of the alcohol and the hydrocarbon, and the reaction is easy to occur.
Example 3 microbiological treatment of lignite to hydrogen production
Inoculating activated Klebsiella aerogenes (Klebsiella aerogenes SKA 1) into a seed culture medium for anaerobic culture, and culturing for 15h at 35 ℃ to obtain Klebsiella aerogenes (Klebsiella aerogenes SKA) seed liquid.
Inoculating the seed solution and the seed culture medium with the volume ratio of 10% (V/V) to the seed culture medium, and shake culturing at 35 ℃ for 15h. Then the cultured seed culture medium, sterilized water and pretreated lignite powder No. 5 winning (4 parts of lignite powder No. 5 winning are respectively treated by alcohols, esters, ethers and hydrocarbon organic solvents) are used as fermentation culture media, respectively sealed and anaerobic fermented for 7 days at 35 ℃. In addition, pulverized coal which is only crushed and not treated by any organic solvent is used as a substrate to be used as a raw coal gas generating set. Pulverized coal, which had been crushed only and not treated with any organic solvent, was used again as a substrate, and no microorganism was inoculated as a blank. Each group ofThe experiment was repeated three times. Anaerobic fermentation gas production is carried out for 7 days, and H is detected once a day 2 Yield.
H between different pretreated victory No. 5 brown coals 2 The generated curve has the same trend, and is between 1 and 4d, H 2 The yield rises rapidly, and the rising speed becomes slow and gradually stabilizes around 5 d. Wherein the pulverized coal (raw coal) H which is not treated by any organic solvent 2 The cumulative yield was 8.91. Mu. Mol/g coal, and the blank group produced little H 2 . Lignite coal sample H treated by different organic solvents 2 The yield is increased to different degrees compared with the raw coal, wherein the H of the lignite coal sample treated by the alcohol organic solvent 2 The yield was highest, reaching 19.13. Mu. Mol/g coal, followed by hydrocarbon organic solvent treatment of the coal (16.02. Mu. Mol/g)>Treatment of coal with an organic solvent of the ester type (14.96. Mu. Mol/g)>The ether organic solvent is used for treating coal (13.06 mu mol/g). Wherein, the lignite coal sample treated by the alcohol organic solvent with the highest hydrogen production amount can produce 0.425 cubic meters of hydrogen per ton of coal.
Wherein the content of lignite powder in the fermentation medium is 80g/L.
The measurement result of hydrogen is shown in fig. 3, and thus it can be seen that the effect of producing hydrogen is optimal when the completely pretreated lignite powder is subjected to anaerobic fermentation by klebsiella aerogenes (Klebsiella aerogenes SKA 1).
Industrial analysis (Table 3) and elemental analysis (Table 4) are performed on the lignite powder before, after and after pretreatment and after gas production, and the results show that the lignite powder is subjected to microbial fermentation and gas production and has no obvious physical and chemical properties, and can be continuously used after subsequent recovery. And part of inorganic sulfur in lignite powder is utilized by microorganisms in the anaerobic fermentation process, so that the problem of environmental pollution of sulfur in the subsequent utilization process can be reduced.
TABLE 3 Industrial analysis of coal before and after gas production
TABLE 4 elemental analysis of coal before and after gas production
Claims (5)
1. A preparation method of a fermentation substrate for producing hydrogen by sulfate reducing bacteria is characterized by comprising the following steps:
the substrate is treated, the substrate is firstly crushed into pieces with granularity smaller than 5mm, and the substrate is pretreated lignite pieces;
mixing substrate scraps with an organic solvent, wherein 1L of the organic solvent is added into every 100g of lignite scraps, and the organic solvent is one of alcohol, ester, hydrocarbon and ether;
ultrasonic extraction is carried out on the mixture of the organic solvent and the substrate for 2 hours under the power of 350W, the extraction of the organic solvent damages the physical morphology of the substrate fragments, and partial inorganic micromolecules are extracted, so that the pores of the substrate fragments become larger and the structure is more loose;
filtering to separate organic solvent liquid and substrate, and heating and drying the substrate to obtain fermentation substrate.
2. Use of a fermentation substrate prepared by the method of claim 1, wherein: mixing the pretreated lignite powder with activated sulfate reducing bacteria seed liquid, and performing anaerobic fermentation in a fermentation substrate to prepare hydrogen; the strain in the sulfate reducing bacteria seed liquid is Klebsiella aerogenesKlebsiella aerogenesThe strain is Brevibacterium, the strain utilizes sulfate as a respiratory chain terminal electron acceptor to reduce the sulfate into sulfide, the logarithmic growth log phase is carried out by 10-36h, the optimal growth pH is 7.0, the optimal temperature is 35 ℃, and the strain is commercially available.
3. The use according to claim 2, characterized in that: the activated sulfate reducing bacteria seed solution consists of sulfate reducing bacteria and a seed culture medium, wherein the seed culture medium comprises the following components in percentage by mass: dipotassium hydrogen phosphate 0.005%, yeast extract 0.01%, sodium sulfate 0.005%, magnesium sulfate heptahydrate 0.02%, cysteine hydrochloride 0.01%, sodium lactate 0.07% at 50%, ammonium chloride 0.01%, calcium chloride dihydrate 0.01%, resazurin 0.01% at 0.1% and water 99.85%.
4. The use according to claim 2, characterized in that: the fermentation medium used for anaerobic fermentation comprises pretreated lignite powder, basic inorganic salt and water; wherein the basic inorganic salt of the fermentation medium comprises the following components in percentage by mass: dipotassium hydrogen phosphate 0.005%, sodium sulfate 0.005%, magnesium sulfate heptahydrate 0.02%, ammonium chloride 0.01%, calcium chloride dihydrate 0.01%, substrate 10% and water 89.95%; the content of lignite powder is 80g/L, and 1.0g of cysteine hydrochloride is additionally added into each liter of the prepared fermentation medium to keep the reducibility in the fermentation process.
5. The use according to claim 2, characterized in that: the fermentation temperature of the substrate in the fermentation culture is 35 ℃, the anaerobic fermentation is carried out, and the fermentation period is 7 days.
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