CN107868802A - A kind of method that bio-ethanol is prepared using agriculture and forestry organic waste material as raw material - Google Patents
A kind of method that bio-ethanol is prepared using agriculture and forestry organic waste material as raw material Download PDFInfo
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- CN107868802A CN107868802A CN201711389975.8A CN201711389975A CN107868802A CN 107868802 A CN107868802 A CN 107868802A CN 201711389975 A CN201711389975 A CN 201711389975A CN 107868802 A CN107868802 A CN 107868802A
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- ethanol
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- organic waste
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention discloses a kind of method that bio-ethanol is prepared using agriculture and forestry organic waste material as raw material, comprise the following steps：Stalk and white wine groove are subjected to rinsing and remove impurity, drying, it is 0.5 4mm to be then crushed to particle diameter；Above-mentioned material is added in 10% aqueous formic acid；Temperature is raised to 120 140 DEG C of 30 120min of reaction；Adjustment gained hydrolyzate pH value is 5.5 6.5；It is standby that filtrate is left after filtering；Add 0.05 0.1% diammonium hydrogen phosphate, 0.3 0.6% magnesium carbonate, 0.5 1.0% urea, 0.3 0.7% peptone, 6 10% high activity saccharomyces cerevisiaes and 0.01 0.03% cellulase, it is 28 38 DEG C to adjust temperature, ferment 30 60h, you can obtains the bio-ethanol.
Description
Technical field
It is more particularly to a kind of to prepare bio-ethanol by raw material of agriculture and forestry organic waste material the invention belongs to new energy materialses field
Method.
Background technology
Stalk is the general name of ripe crops cauline leaf (fringe) part.It is often referred to wheat, rice, corn, potato class, rape, cotton
The remainder of flower, sugarcane and other crops (being usually coarse food grain) after seed is harvested.The photosynthetic product of crops has
More than half is present in stalk, and stalk is rich in nitrogen, phosphorus, potassium, calcium, magnesium and organic matter etc., be it is a kind of have it is multiduty can be again
Raw living resources, but the problems such as under-utilized, efficiency is relatively low at present still be present；In energy shortage instantly, fully application
The waste material development new energy of stalk has realistic meaning.
The content of the invention
For above-mentioned deficiency above-mentioned in the prior art, it is an object of the invention to provide one kind using agriculture and forestry organic waste material as raw material system
The method of standby bio-ethanol, on the one hand can make full use of agriculture and forestry organic waste material, turn waste into wealth, and reduce latency environment pollution；It is another
Aspect is also to open up the important channel of new energy.
To realize that the object of the invention technical scheme is as follows：
A kind of method that bio-ethanol is prepared using agriculture and forestry organic waste material as raw material, comprises the following steps：
S1：50-60 parts stalk and 20-30 part white wine grooves are subjected to rinsing and remove impurity, drying, being then crushed to particle diameter is
0.5-4mm；
S2：Resulting material in step S1 is added in 10% aqueous formic acid, solid-to-liquid ratio 1:10-20；Raise temperature
To 120-140 DEG C of reaction 30-120min；
S3：Hydrolyzate pH value obtained by set-up procedure S2 is 5.5-6.5；It is standby that filtrate is left after filtering；
S4：Added into step S3 0.05-0.1% diammonium hydrogen phosphates, 0.3-0.6% magnesium carbonate, 0.5-1.0% urea,
0.3-0.7% peptones, 6-10% high activities saccharomyces cerevisiae and 0.01-0.03% cellulases, adjustment temperature are 28-38 DEG C,
Ferment 30-60h, you can obtains the bio-ethanol.
Preferably, also include in step S4 after fermentation ends, reaction solution is distilled to obtain high-purity bio-ethanol.
Preferably, 55 parts of stalk described in step S1,25 parts of white wine groove；It is 1-3mm to be crushed to particle diameter.
Preferably, solid-to-liquid ratio described in step S2 is 1:15；Temperature is raised to 125 DEG C of reaction 80min.
Preferably, hydrolyzate pH value described in step S3 is 6.2.
Preferably, 0.07% diammonium hydrogen phosphate, 0.5% magnesium carbonate, 0.8% urea, 0.5% albumen are added in step S4
Peptone, 8% high activity saccharomyces cerevisiae and 0.02% cellulase；It is 35 DEG C to adjust temperature, and ferment 45h.
Preferably, the temperature of the distillation is 90-100 DEG C.
Compared with prior art, its advantage is the present invention：
A kind of method that bio-ethanol is prepared using agriculture and forestry organic waste material as raw material of the present invention, with agriculture and forestry organic waste material stalk and
The waste materials such as white wine groove are main body, the step such as hydrolyze, ferment by cellulose and bio-ethanol is prepared；In preparation technology of the present invention
Under optimal conditions, by adjusting hydrolysis solid-to-liquid ratio, fermented ingredient, conversion and the utilization rate of stalk and white wine groove are improved；And normal temperature
Fermentation, production cost are high；The bio-ethanol of high-purity is can obtain after distilation, meets market different demands.
Embodiment
The present invention is further illustrated with reference to embodiments.
Embodiment 1
S1：50 parts of stalks and 30 parts of white wine grooves are subjected to rinsing and remove impurity, drying, it is 0.5mm to be then crushed to particle diameter；
S2：Resulting material in step S1 is added in 10% aqueous formic acid, solid-to-liquid ratio 1:20；Raise temperature extremely
120 DEG C of reaction 30min；
S3：Hydrolyzate pH value obtained by set-up procedure S2 is 5.5；It is standby that filtrate is left after filtering；
S4：Added into step S3 0.05% diammonium hydrogen phosphate, 0.3% magnesium carbonate, 0.5% urea, 0.3% peptone,
6% high activity saccharomyces cerevisiae and 0.01% cellulase, adjustment temperature are 28 DEG C, and ferment 30h, you can obtains the biological second
Alcohol.
Conversion ratio obtains purity of alcohol as 70% up to 79% in above-mentioned course of reaction.
Embodiment 2
S1：60 parts of stalks and 20 parts of white wine grooves are subjected to rinsing and remove impurity, drying, it is 4mm to be then crushed to particle diameter；
S2：Resulting material in step S1 is added in 10% aqueous formic acid, solid-to-liquid ratio 1:10；Raise temperature extremely
140 DEG C of reaction 120min；
S3：Hydrolyzate pH value obtained by set-up procedure S2 is 6.5；It is standby that filtrate is left after filtering；
S4：Added into step S3 0.1% diammonium hydrogen phosphate, 0.6% magnesium carbonate, 1.0% urea, 0.7% peptone,
10% high activity saccharomyces cerevisiae and 0.03% cellulase, adjustment temperature are 38 DEG C, and ferment 60h, you can obtains the biological second
Alcohol.
Conversion ratio obtains purity of alcohol as 72% up to 82% in above-mentioned course of reaction.
Embodiment 3
S1：52 parts of stalks and 22 parts of white wine grooves are subjected to rinsing and remove impurity, drying, it is 1mm to be then crushed to particle diameter；
S2：Resulting material in step S1 is added in 10% aqueous formic acid, solid-to-liquid ratio 1:12；Raise temperature extremely
130 DEG C of reaction 50min；
S3：Hydrolyzate pH value obtained by set-up procedure S2 is 6；It is standby that filtrate is left after filtering；
S4：Added into step S3 0.08% diammonium hydrogen phosphate, 0.4% magnesium carbonate, 0.7% urea, 0.4% peptone,
8% high activity saccharomyces cerevisiae and 0.02% cellulase, adjustment temperature are 30 DEG C, and ferment 40h, you can obtains the biological second
Alcohol.
Conversion ratio obtains purity of alcohol as 75% up to 83% in above-mentioned course of reaction.
Embodiment 4
S1：58 parts of stalks and 26 parts of white wine grooves are subjected to rinsing and remove impurity, drying, it is 2mm to be then crushed to particle diameter；
S2：Resulting material in step S1 is added in 10% aqueous formic acid, solid-to-liquid ratio 1:18；Raise temperature extremely
135 DEG C of reaction 80min；
S3：Hydrolyzate pH value obtained by set-up procedure S2 is 6.2；It is standby that filtrate is left after filtering；
S4：Added into step S3 0.1% diammonium hydrogen phosphate, 0.5% magnesium carbonate, 0.9% urea, 0.6% peptone,
10% high activity saccharomyces cerevisiae and 0.015% cellulase, adjustment temperature are 35 DEG C, and ferment 50h, you can obtains the biology
Ethanol.
Conversion ratio obtains purity of alcohol as 78% up to 85% in above-mentioned course of reaction.
Embodiment 5
S1：55 parts of stalks and 25 parts of white wine grooves are subjected to rinsing and remove impurity, drying, it is 3mm to be then crushed to particle diameter；
S2：Resulting material in step S1 is added in 10% aqueous formic acid, solid-to-liquid ratio 1:15；Raise temperature extremely
125 DEG C of reaction 80min；
S3：Hydrolyzate pH value obtained by set-up procedure S2 is 6.2；It is standby that filtrate is left after filtering；
S4：Added into step S3 0.07% diammonium hydrogen phosphate, 0.5% magnesium carbonate, 0.8% urea, 0.5% peptone,
8% high activity saccharomyces cerevisiae and 0.02% cellulase, adjustment temperature are 35 DEG C, and ferment 45h；Then to reaction solution in temperature
Distilled to obtain high-purity bio-ethanol at 90-100 DEG C.
Conversion ratio obtains purity of alcohol as 99.9% up to 88% in above-mentioned course of reaction.
The invention is not restricted to embodiment here, those skilled in the art do not depart from the present invention according to the announcement of the present invention
The improvement and modification that category is made all should be within protection scope of the present invention.
Claims (7)
- A kind of 1. method that bio-ethanol is prepared using agriculture and forestry organic waste material as raw material, it is characterised in that comprise the following steps：S1：50-60 parts stalk and 20-30 part white wine grooves are subjected to rinsing and remove impurity, drying, it is 0.5- to be then crushed to particle diameter 4mm；S2：Resulting material in step S1 is added in 10% aqueous formic acid, solid-to-liquid ratio 1:10-20；Raise temperature extremely 120-140 DEG C of reaction 30-120min；S3：Hydrolyzate pH value obtained by set-up procedure S2 is 5.5-6.5；It is standby that filtrate is left after filtering；S4：0.05-0.1% diammonium hydrogen phosphates, 0.3-0.6% magnesium carbonate, 0.5-1.0% urea, 0.3- are added into step S3 0.7% peptone, 6-10% high activities saccharomyces cerevisiae and 0.01-0.03% cellulases, adjustment temperature are 28-38 DEG C, fermentation 30-60h, you can obtain the bio-ethanol.
- A kind of 2. method that bio-ethanol is prepared using agriculture and forestry organic waste material as raw material according to claim 1, it is characterised in that Also include in step S4 after fermentation ends, reaction solution is distilled to obtain high-purity bio-ethanol.
- A kind of 3. method that bio-ethanol is prepared using agriculture and forestry organic waste material as raw material according to claim 1, it is characterised in that 55 parts of stalk described in step S1,25 parts of white wine groove；It is 1-3mm to be crushed to particle diameter.
- A kind of 4. method that bio-ethanol is prepared using agriculture and forestry organic waste material as raw material according to claim 1, it is characterised in that Solid-to-liquid ratio described in step S2 is 1:15；Temperature is raised to 125 DEG C of reaction 80min.
- A kind of 5. method that bio-ethanol is prepared using agriculture and forestry organic waste material as raw material according to claim 1, it is characterised in that Hydrolyzate pH value described in step S3 is 6.2.
- A kind of 6. method that bio-ethanol is prepared using agriculture and forestry organic waste material as raw material according to claim 1, it is characterised in that 0.07% diammonium hydrogen phosphate, 0.5% magnesium carbonate, 0.8% urea, 0.5% peptone, the wine brewing of 8% high activity are added in step S4 Yeast and 0.02% cellulase；It is 35 DEG C to adjust temperature, and ferment 45h.
- A kind of 7. method that bio-ethanol is prepared using agriculture and forestry organic waste material as raw material according to claim 2, it is characterised in that The temperature of the distillation is 90-100 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711389975.8A CN107868802A (en) | 2017-12-21 | 2017-12-21 | A kind of method that bio-ethanol is prepared using agriculture and forestry organic waste material as raw material |
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| CN201711389975.8A CN107868802A (en) | 2017-12-21 | 2017-12-21 | A kind of method that bio-ethanol is prepared using agriculture and forestry organic waste material as raw material |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009155673A1 (en) * | 2008-06-23 | 2009-12-30 | Ctc - Centro De Tecnologia Canavieira | Fermentative process for lignocellulosic plant biomass |
| CN101701225A (en) * | 2009-11-17 | 2010-05-05 | 中国海洋大学 | Method for preparing bio-ethanol by taking seaweed processing waste as raw material |
| CN102399826A (en) * | 2011-10-20 | 2012-04-04 | 清华大学 | Comprehensive utilizing method of sweet sorghum stalks |
| CN105567745A (en) * | 2016-01-21 | 2016-05-11 | 天津大学 | Method for improving utilization of xylose in lignocellulose hydrolysate |
-
2017
- 2017-12-21 CN CN201711389975.8A patent/CN107868802A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009155673A1 (en) * | 2008-06-23 | 2009-12-30 | Ctc - Centro De Tecnologia Canavieira | Fermentative process for lignocellulosic plant biomass |
| CN101701225A (en) * | 2009-11-17 | 2010-05-05 | 中国海洋大学 | Method for preparing bio-ethanol by taking seaweed processing waste as raw material |
| CN102399826A (en) * | 2011-10-20 | 2012-04-04 | 清华大学 | Comprehensive utilizing method of sweet sorghum stalks |
| CN105567745A (en) * | 2016-01-21 | 2016-05-11 | 天津大学 | Method for improving utilization of xylose in lignocellulose hydrolysate |
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