CN102146419A

CN102146419A - Method for producing ethanol by using straw as raw material

Info

Publication number: CN102146419A
Application number: CN2010105765726A
Authority: CN
Inventors: 朱纪伍; 徐双发
Original assignee: Individual
Current assignee: Individual
Priority date: 2010-12-07
Filing date: 2010-12-07
Publication date: 2011-08-10

Abstract

The invention provides a method for producing ethanol by using straw as a raw material. The method comprises the following steps of: (1) crushing and pulping: grinding the plant straw into pulp; (2) saccharification: performing saccharification on the pulp of the step 1 by using xylanase at the temperature of between 35 and 38 DEG C; (3) inoculating 12 to 13 percent of pulp into a fermentation medium to perform fermentation, and fermenting the saccharification solution with initial sugar concentration of 8 to 9 percent at the temperature of between 28 and 29 DEG C for 80 to 82 hours; and (4) distillation: performing tower type or column type distillation in industrialized production. The straw is prepared into the straw pulp so that the transport is convenient and the subsequent saccharification and fermentation steps are favorable, and the method has the following advantages besides the advantages of a common method for preparing the ethanol by using the straw. 4 to 10 kilograms of ethanol can be produced by using corn straw; and the saccharified residue has good palatability and can be used for animal feed.

Description

A kind of is raw material production alcoholic acid method with the stalk

Technical field

The present invention relates to a kind of production method of liquid fuel, specifically relating to a kind of is raw material production alcoholic acid method with the stalk.

Background technology

Nearly 600,000,000 tons of the wastes straw of the annual all kinds of farm crop of China, aboundresources, cheap.The cellulosic biological fermentation is produced ethanol can not carry out suitability for industrialized production, and this name of the game is that Financial cost is difficult to reach a standard, and does not have high efficiency processing condition.It is reported that the maximum problem that alcohol industry faces is raw material, cost and three problems of pollution.

Solving the fermentation alcohol raw material is to utilize fibrous material to replace grain; Alcohol Production combines with fodder production; Utilize the gene engineering seed selection can direct fermentation the bacterial strain of Mierocrystalline cellulose, hemicellulose, and thermotolerant yeast, anti-assorted bacterium zymamsis bacterium etc.At present, fermentation cellulosic biomass is converted into alcoholic acid research and still has certain gap apart from practical application, causes the reason of this problem that many aspects are arranged, and mainly shows the pretreated energy consumption height of cellulose raw material; The production cost height of cellulase, enzyme is lived low; Cellulase hydrolysis and yeast-leavened optimum temperuture are inharmonious, and zymotechnique is also not overripened; Production concentration is low, causes product recovery cost height.

Have serious crisis such as coal, petroleum-based energy etc. and causing under the situation of serious environmental pollution, alcohol fuel is renewable with it, non-environmental-pollution, use technology advantage such as become better and approaching perfection day by day enjoys people to pay attention in motor vehicle.Yet traditional production of fuel ethanol adopts that mainly starchy material such as wheat, corn, potato are done etc., perhaps saccharine material such as cane molasses, beet sirup etc., and production cost is higher.The vegetable fibre resource is very abundant, mainly is countryside kitchen work usefulness, and part is the field also, makes energy usefulness by gasification, curing on a small quantity, utilizes level low, serious waste of resources, and the environmental pollution that causes is serious.

Adopt stalk to make raw material production ethanol and will all will produce good benefit resource and society.It is raw material production alcoholic acid method that the prior art report has with the vegetable fibre, and its processing step is as follows: with the agricultural crop straw pulverizing, to lignin degradation processing, saccharification, filtration, sterilization, fermentation and distillation.Though above-mentioned technology can be utilized preparing alcohol from stress or stalk of agricultural crops, because the pretreatment process of raw material exists drawback, influence the alcoholic acid output capacity, to such an extent as to make production alcoholic acid cost too high, stalk can not be fully used.

Summary of the invention

The purpose of this invention is to provide a kind of pretreatment process and improve, the technology of the preparing fuel ethanol by using straw fiber materials that improve the ethanol output capacity, reduces cost stalk fibrid raw material.

Provided by the invention is raw material production alcoholic acid method with the stalk, and said method may further comprise the steps:

(1) handle early stage: straw is milled to slurry and pH value is transferred to 5.5-7.0, was sterilized 21-30 minute down at 100-115 ℃;

(2) saccharification: with zytase the slurry of step 1 is carried out saccharification in 35-38 ℃, remove by filter the insoluble solid matter in the saccharification liquid;

(3) insert fermention medium by the inoculum size of 12-13% and ferment, to initial sugared concentration be 8-9% saccharification liquid at 28-29 ℃ of condition bottom fermentation, the cycle is 80-82 hour;

(4) distillation: originally operate in and adopt tower in the suitability for industrialized production or the pillar distillation.

Provided by the invention is in raw material production alcoholic acid first preferred method with the stalk, it is characterized in that described:

(1) in the processing in early stage: pH value is transferred to 6.0, was sterilized 21-30 minute down at 115 ℃;

(2) in the saccharification step: under 37 ℃, the slurry of step 1 is carried out saccharification, remove by filter the insoluble solid matter in the saccharification liquid with zytase;

(3) insert fermention medium by 12% inoculum size and ferment, to initial sugared concentration be 9% saccharification liquid 29 ℃ of fermentations, the cycle is 80 hours.

In described production alcoholic acid second preferred method provided by the invention, said substratum is: 3, production alcoholic acid method according to claim 3, it is characterized in that: said substratum is, by g/100ml: MgSO ₄7H ₂O:0.05; CaCl ₂: H ₂O:0.01; KH ₂PO ₄: 0.1; (NH ₄) ₂SO ₄: 0.5; NaCl:0.01; Agar: 2.0; And PH:4.5-4.8.

In the technical scheme provided by the invention, solid straws is ground pulping, adopt the liquid crude enzyme liquid degradation of rice straw of biological method, utilize the complete of microbial destruction lignocellulose structure and make the part xylogen be decomposed into carbonic acid gas and water fully, thereby lignocellulose is easy to the treatment stage of saccharification by the cellulase effect.

Pretreated concrete steps

With the stalk slurry, carry out saccharification in 37 ℃, PH 5 and solid-to-liquid ratio 13 times with zytase, reducing sugar.Under 30 ℃, is the saccharification liquid fermentation 72 hour of 7-8% to sugar concentration with pachysolen tannophilus (Pachysolen tannophilus), distill ethanol.

Compared with the prior art, the present invention has following excellent effect:

Stalk is made the stalk slurry except that being convenient to carry, also help follow-up saccharification and fermentation step, to be the present invention prepare outstanding advantage the following beneficial effect that ethanol has except that having the general using stalk for this:

As biomass liquid fuel---the rich raw material sources of alcohol is cheap renewable energy source resource, has widened the approach of waste utilization.

Adopt the maize straw preparing alcohol, not only saved grain, simultaneously maize straw is fully utilized, can receive higher benefit.Corn can be received 500 kilograms in grain for every mu, and maize straw is per ton, can produce ethanol 4-10 kilogram; The slag of saccharification, good palatability can be made the cattle and sheep feed.

Embodiment

A kind of stalk fibrid raw material is produced alcoholic acid technology, comprise: (1) preparation stalk slurries: fresh straw is added amount of water make straw pulp, (2) saccharification: is under 12 conditions stalk slurries to be carried out saccharification with zytase at 38 ℃, PH5.0, liquid-solid ratio; (3) to remove behind the insolubles saccharification liquid under 120 ℃ of conditions, sterilize 20 minutes (4) with minimum medium pachysolen tannophilus (Pachysolen tannophilus) is cultivated, the inoculum size by 10% inserts fermentation culture and fermented 72 hours based on 30 ℃; Said minimum medium is that said substratum is: by g/100ml: MgSO47H ₂O:0.05; CaCl ₂: H ₂O:0.01; KH ₂PO ₄: 0.1; (NH ₄) ₂SO ₄: 0.5; NaCl:0.01; Agar 2.0; PH:4.5-4.8.

Claims

1. one kind is raw material production alcoholic acid method with the stalk, and said method may further comprise the steps:

(3) insert fermention medium by the inoculum size of 12-13% and ferment, to initial sugared concentration be 8-9% saccharification liquid 29 ℃ of fermentations, the cycle is 80-82 hour;

2. be raw material production alcoholic acid method with the stalk according to a kind of of claim 1, it is characterized in that described:

3. production alcoholic acid method according to claim 3 is characterized in that: said substratum is, by g/100ml:

MgSO ₄·7H ₂O：0.05；

CaCl ₂：·H ₂O：0.01；

KH ₂PO ₄：0.1；

(NH ₄) ₂SO ₄：0.5；

NaCl：0.01；

Agar: 2.0; With

PH：4.5-4.8。