CN105925622A - Method for producing ethanol by utilizing bagasse hemicellulose - Google Patents

Method for producing ethanol by utilizing bagasse hemicellulose Download PDF

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CN105925622A
CN105925622A CN201610519108.0A CN201610519108A CN105925622A CN 105925622 A CN105925622 A CN 105925622A CN 201610519108 A CN201610519108 A CN 201610519108A CN 105925622 A CN105925622 A CN 105925622A
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bagasse
hemicellulose
ethanol
bagasse hemicellulose
fermentation
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张聪聪
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

The invention relates to a method for producing ethanol by utilizing bagasse hemicellulose. The method comprises the following steps of: pretreating bagasse, carrying out detoxification treatment on bagasse hemicellulose extracting solution, hydrolyzing the bagasse hemicellulose extracting solution and fermenting bagasse hemicellulose hydrolysate. Double enzyme saccharified bagasse hemicellulose and double yeast mixed strain are adopted for fermenting the bagasse hemicellulose diluted acid hydrolysate, the defect that the traditional saccharomyces cerevisiae can produce ethanol by utilizing glucose only while xylose fermentation can not be utilized is overcome, utilization rate of lignocellulosic materials is greatly improved, and the advantages of high ethanol yield, high conversion rate and the like are shown, so that the method provided by the invention is significant on production of bioethanol, comprehensive utilization of bagasse resource is realized, comprehensive economic benefit of a saccharose enterprise can be obviously improved, and double win in environmental benefit and economic benefit is realized.

Description

Bagasse hemicellulose produces the method for ethanol
Technical field
The invention belongs to biochemical industry, fermentation engineering and biomass energy source domain, particularly relate to a kind of bagasse half fiber Element produces the method for ethanol.
Background technology
Bagasse is the maximum amount of by-product of cane sugar factory, and its productivity is the 24%-27% of processing Caulis Sacchari sinensis amount.China is the world Three cane planting big countries, the yield at southern area bagasse reaches 7000 kt/a.Bagasse is a kind of abundant renewable biomass Resource, it is mainly composed of the natural high molecular substances such as cellulose, hemicellulose and lignin.Wherein hemicellulose is mainly The xylan being polymerized with xylose, arabinose, cellulose is then the polymer of glucose.These bagasses are except a small amount of at present Outside papermaking, the overwhelming majority is burned off or discarded, not only causes the wasting of resources, goes back serious environment pollution.Therefore, if can be from The hemicellulose and the cellulose that extract high-quality in bagasse are applied to commercial production and will produce huge economic benefit and life State is imitated.Bagasse can be used as in addition to papermaking except part with bagasse, mostly as garbage or as boiler oil, utilization rate is the highest. If bagasse sufficiently can be comprehensively utilized by biomass trans-utilization engineering, will significantly improve its economic worth and The value of environmental protection.Ethanol is the most promising liquid fuel from biomass resource.Bagasse is considered as a kind of source Abundant Renewable resource, is one of important source material as cellulose fuel ethanol.But at present, bagasse produces ethanol Conversion ratio is the lowest.One of them major reason is that the utilization rate of Bagasse Hemicellulose is the lowest.In ethanol production process, Mainly make use of the cellulose in sugarcane fiber raw material, and most hemicellulose and lignin can be dissolved in when pretreatment In treatment fluid, can not get effectively utilizing.Therefore, how to improve in the utilization rate of Bagasse Hemicellulose, remove lignin as far as possible Become an important and urgent problem of bagasse comprehensive utilization.
Summary of the invention
It is an object of the invention to provide a kind of production cost low, the bagasse hemicellulose that alcohol yied is high, conversion ratio is fast is raw The method of producing and ethanol, it is achieved the comprehensive utilization of bagasse resource, it is possible to significantly improve the overall economic efficiency of cane suger enterprise, it is achieved ring Border benefit and economic benefit doulbe-sides' victory.
The present invention is achieved in that
A kind of bagasse hemicellulose produces the method for ethanol, it is characterised in that: comprise the following steps:
(1) bagasse pretreatment: described bagasse pretreatment is to smash by after bagasse and the mixing of Caulis Sacchari sinensis end pin with pulverizer, cross 40- 80 eye mesh screens, it is to process in 0.1%-1% sulfuric acid solution that the mixture after then smashing immerses mass fraction, and solid-to-liquid ratio is 1: 10-20, steaming and decocting 2-10 minute at 170 DEG C-200 DEG C, filter, prepare bagasse hemicellulose extracting solution；
(2) bagasse hemicellulose extracting solution detoxification treatment: add calcium oxide regulation hydrolyzed solution pH in bagasse hemicellulose extracting solution To 9-11, then heating hydrolyzed solution is to 50 DEG C-60 DEG C, is incubated 20-30 minute, filters, and hydrolyzed solution is through activated carbon adsorption, then uses sulfur Acid readjustment pH to 5.5-6.0；
(3) bagasse hemicellulose extracting solution hydrolysis: be placed in retort by the bagasse hemicellulose extracting solution after detoxification treatment, adjusts Joint pH=4.8-5.0, and add cellulase and xylanase mixed vaccine and seal and be placed in shaking table reaction, at 30 DEG C-45 DEG C At a temperature of hydrolysis and saccharification 50-80 hour, i.e. complete Enzymatic hydrolysis process；
(4) bagasse hemicellulose hydrolyzed solution fermentation: fixing for absorption material is fixed in fermentation tank, then Caulis Sacchari sinensis fiber element is hydrolyzed Liquid proceeds in fermentation tank, adds appropriate basic nutrient solution, after the fixing abundant imbibition of material of absorption, and inoculation yeast bacterial strain, then control Fermentation temperature processed is 35-38 DEG C, pH=6, and ferment 20-30h, and the post processing fermentation liquid that fermented obtains ethanol.
Further, the weight ratio of described bagasse and Caulis Sacchari sinensis end pin is 6-7:2-3.
Further, the consumption of described cellulase and xylanase mixed vaccine is 1.5-2.0g/L.
Further, the weight ratio of described cellulase and xylanase is 6-7:3-4.
Further, the preparation method of described cellulase is: is 25-35 DEG C in temperature, under conditions of pH=4.0-5.0, uses Trichoderma reesei is as host cell secretes cellulase, and wherein, using tobacco waste as carbon source, soybean cake powder is nitrogen source, cultivates Time is 6-10 hour, ventilation 0.4-1.0vvm, and mixing speed is 200-500 rev/min.
Further, the preparation method of described xylanase is: produces in bacterial strain with xylanase and picks out aspergillus niger mutation Strain, as producing bacterial strain, carries out solid fermentation in koji plate fermentation culture medium, it is thus achieved that after leaven material buffer leaven material Liquid extracts, it is thus achieved that xylanase liquid；Consisting of of described koji plate fermentation culture medium: koji tray dress 100g base material, (NH4)2SO4 0.5-1.5g, peptone 0.5-1g, KH2PO4 0.3-0.4g, CaCl2 0.2-0.3g, ZnCl2 0.1-0.2g, MgSO40.1- 0.2g, Fe2(SO4)3 0.02-0.08g, polysorbate40 0.4-0.8 g, tap water 120-140mL, natural pH, the wherein group of base material Become wheat bran 50-80 g, corn cob 10-40g, soybean cake powder 10-20g.
Further, in described step (4), yeast strain is pichia stipitis(Pichia stipitis)False with Xiu Hata Silk yeast(Candida shehatae)Hybrid bacterial strain, inoculum concentration is 2.g/L, pichia stipitis and shehatae candida Weight ratio is 1-2:1.Described pichia stipitis and shehatae candida, be to purchase from Organism Depositary or market Buy, cultivate through domestication so that it is adapt to bagasse hemicellulose hydrolyzed solution.
Further, each composition proportion of basic nutrient solution in described step (4) is: addition g/L calculates: (NH4)2SO4 2g/L, MnSO40.1g/L, yeast powder 2g/L, Semen Maydis pulp 20g/L, K2HPO4 0.2g/L, CaCO3 0.2g/L。
Further, described absorption fixes material for rich in cancellated Plant fiber, rich in cancellated artificial fibre Dimension or metal mesh material.
The substantive distinguishing features that the present invention highlights with significantly progress is:
1, the present invention uses pichia stipitis and shehatae candida hybrid bacterial strain to produce ethanol, and production cost is low, ethanol Yield is high, and ethanol production is up to 22.85 g/L, and the volume fraction of ethanol of fermenting-ripening mash is 13.5%, alcohol getting rate 93.5%, Realize the comprehensive utilization of bagasse resource, it is possible to significantly improve the overall economic efficiency of cane suger enterprise, it is achieved environmental benefit and economy Benefit doulbe-sides' victory.
2, the present invention uses pichia stipitis and shehatae candida hybrid bacterial strain fermentation hemicellulose dilute acid hydrolysis Liquid not only solves traditional saccharomyces cerevisiae can not utilize the defect of xylose fermentation for producing ethanol only with glucose, significantly carries The high utilization rate of lignocellulose raw material, and show the advantages such as alcohol yied is high, conversion ratio is fast, the life to bio-ethanol Produce significant.
3 present invention add a certain amount of Caulis Sacchari sinensis end pin on Bagasse Material, by increasing capacitance it is possible to increase the content of hemicellulose in raw material And sugar content, improve enzymatic hydrolyzation, reduce production cost.
4 use cellulase and xylanase that bagasse hemicellulose is carried out double enzymes saccharification, it is possible to reduce product greatly Inhibitory action and the further degraded of product, not only increase enzymolysis yield so that the enzymolysis efficiency of bagasse obtains bigger Improve, decrease the consumption of enzyme, reduce production cost.
5 present invention use sulphuric acid hot-water pretreatment method to extract the hemicellulose in plant fiber material to have low cost, right Environmental nonpollution, energy consumption are low, the percent hydrolysis of hemicellulose and response rate advantages of higher, have good development prospect.
6, the present invention uses absorption fixing material to be fixed up by yeast strain to carry out alcohol fermentation, be possible not only to increase yeast Strain density, and can be repeatedly circulated, reduce the sugar that Yeast proliferation consumes, improve ferment strength and products collection efficiency.
Detailed description of the invention
Embodiment 1
Bagasse hemicellulose described in the present embodiment produces the method for ethanol, mainly comprises the steps that
(1) bagasse pretreatment: described bagasse pretreatment is to smash by after bagasse and the mixing of Caulis Sacchari sinensis end pin with pulverizer, cross 40- 80 eye mesh screens, the weight ratio of bagasse and Caulis Sacchari sinensis end pin is 6:2, and it is 0.1% sulfur that the mixture after then smashing immerses mass fraction Processing in acid solution, solid-to-liquid ratio is 1:10, steaming and decocting 2-10 minute at 170 DEG C, filters, and prepares bagasse hemicellulose extracting solution；
(2) bagasse hemicellulose extracting solution detoxification treatment: add calcium oxide regulation hydrolyzed solution pH in bagasse hemicellulose extracting solution To 9, then heating hydrolyzed solution is to 50 DEG C, is incubated 20-30 minute, filters, and hydrolyzed solution is through activated carbon adsorption, then adjusts back pH with sulphuric acid To 5.5-6.0；
(3) bagasse hemicellulose extracting solution hydrolysis: be placed in retort by the bagasse hemicellulose extracting solution after detoxification treatment, adjusts Joint pH=4.8-5.0, and add cellulase and xylanase mixed vaccine and seal and be placed in shaking table reaction, i.e. complete enzyme hydrolysis Saccharifying；The consumption of cellulase and xylanase mixed vaccine is 1.5g/L, described cellulase and the weight of xylanase Ratio is 6:3, hydrolysis and saccharification 50-80 hour at a temperature of 30 DEG C,
(4) bagasse hemicellulose hydrolyzed solution fermentation: will be enriched in cancellated Plant fiber and be fixed in fermentation tank, then by Caulis Sacchari sinensis Cellulosic hydrolysate proceeds in fermentation tank, adds appropriate basic nutrient solution, after the abundant imbibition of cancellated Plant fiber, Inoculation yeast bacterial strain, yeast strain is pichia stipitis and shehatae candida hybrid bacterial strain, and inoculum concentration is 2g/L, trunk The weight ratio of Pichia sp. and shehatae candida is 1:1.Then control fermentation temperature and be 35-38 DEG C, pH=6, ferment 20h, The post processing fermentation liquid that fermented obtains ethanol.
Embodiment 2
Bagasse hemicellulose described in the present embodiment produces the method for ethanol, mainly comprises the steps that
(1) bagasse pretreatment: described bagasse pretreatment is to smash by after bagasse and the mixing of Caulis Sacchari sinensis end pin with pulverizer, cross 40- 80 eye mesh screens, the weight ratio of bagasse and Caulis Sacchari sinensis end pin is 7:2, and it is 0.5 sulfur that the mixture after then smashing immerses mass fraction Acid solution processes, solid-to-liquid ratio be 1:1418 DEG C at steaming and decocting 2-10 minute, filter, prepare bagasse hemicellulose extracting solution；
(2) bagasse hemicellulose extracting solution detoxification treatment: add calcium oxide regulation hydrolyzed solution pH in bagasse hemicellulose extracting solution To 10, then heating hydrolyzed solution is to 55 DEG C, is incubated 20-30 minute, filters, and hydrolyzed solution is through activated carbon adsorption, then adjusts back with sulphuric acid PH to 5.5-6.0；
(3) bagasse hemicellulose extracting solution hydrolysis: be placed in retort by the bagasse hemicellulose extracting solution after detoxification treatment, adjusts Joint pH=4.8-5.0, and add cellulase and xylanase mixed vaccine and seal and be placed in shaking table reaction, i.e. complete enzyme hydrolysis Saccharifying；The consumption of cellulase and xylanase mixed vaccine is 1.6g/L, described cellulase and the weight of xylanase Ratio is 7:3, hydrolysis and saccharification 50-80 hour at a temperature of 35 DEG C,
(4) bagasse hemicellulose hydrolyzed solution fermentation: will be enriched in cancellated Plant fiber and be fixed in fermentation tank, then by Caulis Sacchari sinensis Cellulosic hydrolysate proceeds in fermentation tank, adds appropriate basic nutrient solution, after the abundant imbibition of cancellated Plant fiber, Inoculation yeast bacterial strain, yeast strain is pichia stipitis and shehatae candida hybrid bacterial strain, and inoculum concentration is 2g/L, trunk The weight ratio of Pichia sp. and shehatae candida is 2:1.Then control fermentation temperature and be 35-38 DEG C, pH=6, ferment 25h, The post processing fermentation liquid that fermented obtains ethanol.
Embodiment 3
Bagasse hemicellulose described in the present embodiment produces the method for ethanol, mainly comprises the steps that
(1) bagasse pretreatment: described bagasse pretreatment is to smash by after bagasse and the mixing of Caulis Sacchari sinensis end pin with pulverizer, cross 40- 80 eye mesh screens, the weight ratio of bagasse and Caulis Sacchari sinensis end pin is 6:3, and it is 0.8% sulfur that the mixture after then smashing immerses mass fraction Processing in acid solution, solid-to-liquid ratio is 1:16 steaming and decocting 2-10 minute at 19 DEG C, filters, and prepares bagasse hemicellulose extracting solution；
(2) bagasse hemicellulose extracting solution detoxification treatment: add calcium oxide regulation hydrolyzed solution pH in bagasse hemicellulose extracting solution To 11, then heating hydrolyzed solution is to 58 DEG C, is incubated 20-30 minute, filters, and hydrolyzed solution is through activated carbon adsorption, then adjusts back with sulphuric acid PH to 5.5-6.0；
(3) bagasse hemicellulose extracting solution hydrolysis: be placed in retort by the bagasse hemicellulose extracting solution after detoxification treatment, adjusts Joint pH=4.8-5.0, and add cellulase and xylanase mixed vaccine and seal and be placed in shaking table reaction, i.e. complete enzyme hydrolysis Saccharifying；The consumption of cellulase and xylanase mixed vaccine is 1.8g/L, described cellulase and the weight of xylanase Ratio is 6:4, hydrolysis and saccharification 50-80 hour at a temperature of 40 DEG C,
(4) bagasse hemicellulose hydrolyzed solution fermentation: will be enriched in cancellated staple fibre and be fixed in fermentation tank, then by Caulis Sacchari sinensis Cellulosic hydrolysate proceeds in fermentation tank, adds appropriate basic nutrient solution, after the abundant imbibition of cancellated staple fibre, Inoculation yeast bacterial strain, yeast strain is pichia stipitis and shehatae candida hybrid bacterial strain, and inoculum concentration is 2g/L, trunk The weight ratio of Pichia sp. and shehatae candida is 1:1.Then control fermentation temperature and be 35-38 DEG C, pH=6, ferment 28h, The post processing fermentation liquid that fermented obtains ethanol.
Embodiment 4
Bagasse hemicellulose described in the present embodiment produces the method for ethanol, mainly comprises the steps that
(1) bagasse pretreatment: described bagasse pretreatment is to smash by after bagasse and the mixing of Caulis Sacchari sinensis end pin with pulverizer, cross 40- 80 eye mesh screens, the weight ratio of bagasse and Caulis Sacchari sinensis end pin is 7:3, and it is 1% sulphuric acid that the mixture after then smashing immerses mass fraction Processing in solution, solid-to-liquid ratio is 1:20, steaming and decocting 2-10 minute at 200 DEG C, filters, and prepares bagasse hemicellulose extracting solution；
(2) bagasse hemicellulose extracting solution detoxification treatment: add calcium oxide regulation hydrolyzed solution pH in bagasse hemicellulose extracting solution To 11, then heating hydrolyzed solution is to 60 DEG C, is incubated 20-30 minute, filters, and hydrolyzed solution is through activated carbon adsorption, then adjusts back with sulphuric acid PH to 5.5-6.0；
(3) bagasse hemicellulose extracting solution hydrolysis: be placed in retort by the bagasse hemicellulose extracting solution after detoxification treatment, adjusts Joint pH=4.8-5.0, and add cellulase and xylanase mixed vaccine and seal and be placed in shaking table reaction, i.e. complete enzyme hydrolysis Saccharifying；The consumption of cellulase and xylanase mixed vaccine is 2.0g/L, described cellulase and the weight of xylanase Ratio is 7:4, hydrolysis and saccharification 50-80 hour at a temperature of 45 DEG C,
(4) bagasse hemicellulose hydrolyzed solution fermentation: metal mesh material is fixed in fermentation tank, then Caulis Sacchari sinensis fiber element is hydrolyzed Liquid proceeds in fermentation tank, adds appropriate basic nutrient solution, after the abundant imbibition of metal mesh material, and inoculation yeast bacterial strain, yeast Strain is pichia stipitis and shehatae candida hybrid bacterial strain, and inoculum concentration is 2g/L, and pichia stipitis and Xiu Hata are false The weight ratio of silk yeast is 2:1.Then controlling fermentation temperature and be 35-38 DEG C, pH=6, ferment 30h, and post processing of having fermented is fermented Liquid obtains ethanol.

Claims (9)

1. the method that a bagasse hemicellulose produces ethanol, it is characterised in that: comprise the following steps:
(1) bagasse pretreatment: described bagasse pretreatment is to smash by after bagasse and the mixing of Caulis Sacchari sinensis end pin with pulverizer, cross 40- 80 eye mesh screens, it is to process in 0.1%-1% sulfuric acid solution that the mixture after then smashing immerses mass fraction, and solid-to-liquid ratio is 1: 10-20, steaming and decocting 2-10 minute at 170 DEG C-200 DEG C, filter, prepare bagasse hemicellulose extracting solution；
(2) bagasse hemicellulose extracting solution detoxification treatment: add calcium oxide regulation hydrolyzed solution pH in bagasse hemicellulose extracting solution To 9-11, then heating hydrolyzed solution is to 50 DEG C-60 DEG C, is incubated 20-30 minute, filters, and hydrolyzed solution is through activated carbon adsorption, then uses sulfur Acid readjustment pH to 5.5-6.0；
(3) bagasse hemicellulose extracting solution hydrolysis: be placed in retort by the bagasse hemicellulose extracting solution after detoxification treatment, adjusts Joint pH=4.8-5.0, and add cellulase and xylanase mixed vaccine and seal and be placed in shaking table reaction, at 30 DEG C-45 DEG C At a temperature of hydrolysis and saccharification 50-80 hour, i.e. complete Enzymatic hydrolysis process；
(4) bagasse hemicellulose hydrolyzed solution fermentation: fixing for absorption material is fixed in fermentation tank, then Caulis Sacchari sinensis fiber element is hydrolyzed Liquid proceeds in fermentation tank, adds appropriate basic nutrient solution, after the fixing abundant imbibition of material of absorption, and inoculation yeast bacterial strain, then control Fermentation temperature processed is 35-38 DEG C, pH=6, and ferment 20-30h, and the post processing fermentation liquid that fermented obtains ethanol.
Bagasse hemicellulose the most according to claim 1 produces the method for ethanol, it is characterised in that: described bagasse and Caulis Sacchari sinensis The weight ratio of end pin is 6-7:2-3.
Bagasse hemicellulose the most according to claim 1 produce ethanol method, it is characterised in that: described cellulase and The consumption of xylanase mixed vaccine is 1.5-2.0g/L.
Bagasse hemicellulose the most according to claim 1 produce ethanol method, it is characterised in that: described cellulase and The weight ratio of xylanase is 6-7:3-4.
Bagasse hemicellulose the most according to claim 1 produces the method for ethanol, it is characterised in that: described cellulase Preparation method is: temperature be 25-35 DEG C, under conditions of pH=4.0-5.0, with trichoderma reesei as host cell secretes fiber Element enzyme, wherein, using tobacco waste as carbon source, soybean cake powder is nitrogen source, and incubation time is 6-10 hour, ventilation 0.4- 1.0vvm, mixing speed is 200-500 rev/min.
Bagasse hemicellulose the most according to claim 1 produces the method for ethanol, it is characterised in that: described xylanase Preparation method is: produces in bacterial strain using xylanase and picks out aspergillus niger mutagenic fungi as producing bacterial strain, cultivates at koji plate fermentation Solid fermentation is carried out, it is thus achieved that after leaven material, leaven material buffer is extracted, it is thus achieved that xylanase liquid on base；Described Consisting of of koji plate fermentation culture medium: koji tray dress 100g base material, (NH4)2SO4 0.5-1.5g, peptone 0.5-1g, KH2PO4 0.3-0.4g, CaCl2 0.2-0.3g, ZnCl2 0.1-0.2g, MgSO40.1-0.2g, Fe2(SO4)3 0.02-0.08g, polysorbate40 0.4-0.8 g, tap water 120-140mL, natural pH, wherein base material consist of wheat bran 50-80 g, corn cob 10-40g, bean Cake powder 10-20g.
Bagasse hemicellulose the most according to claim 1 produces the method for ethanol, it is characterised in that: in described step (4) Yeast strain is pichia stipitis and shehatae candida hybrid bacterial strain, and inoculum concentration is 2g/L, pichia stipitis and stop The weight ratio breathing out tower candida mycoderma is 1-2:1.
Bagasse hemicellulose the most according to claim 1 produces the method for ethanol, it is characterised in that: in described step (4) The each composition proportion of basic nutrient solution be: addition g/L calculate: (NH4)2SO4 2g/L, MnSO40.1g/L, yeast powder 2g/L, Semen Maydis pulp 20g/L, K2HPO4 0.2g/L, CaCO3 0.2g/L。
Bagasse hemicellulose the most according to claim 1 produces the method for ethanol, it is characterised in that: material is fixed in described absorption Material is for rich in cancellated Plant fiber, rich in cancellated staple fibre or metal mesh material.
CN201610519108.0A 2016-07-05 2016-07-05 Method for producing ethanol by utilizing bagasse hemicellulose Pending CN105925622A (en)

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Cited By (2)

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CN106811553A (en) * 2017-02-09 2017-06-09 合肥原素坊食品有限公司 A kind of preparation method of prebiotics brown sugar
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CN109280679A (en) * 2018-10-30 2019-01-29 华南农业大学 A method of Heating Explosion Sugarcane Bagasse efficiency is improved by the metal salt pretreatment of reinforced by additive

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