CN102250974A - Preparation method of microbial oil - Google Patents
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- CN102250974A CN102250974A CN201010176591XA CN201010176591A CN102250974A CN 102250974 A CN102250974 A CN 102250974A CN 201010176591X A CN201010176591X A CN 201010176591XA CN 201010176591 A CN201010176591 A CN 201010176591A CN 102250974 A CN102250974 A CN 102250974A
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Abstract
The invention discloses a method for preparing microbial oil. The method comprises the following steps of: performing aerated culturing on a carbon source which contains glucose and xylose and serves as a raw material to obtain a leavening; and extracting the microbial oil from the leavening. According to the method, glucose and xylose in a culture medium can be transformed synchronously, so that the inhibition function of the glucose on the utilization of other substrates during the utilization of mixed sugar is relieved, the phenomenon of secondary growth in the process is avoided, the substrate transformation rate is high, and the production cost can be lowered remarkably. The method has a great application prospect in the aspect of the production of microbial oil with a lignocellulose material.
Description
Technical field
The invention belongs to the production of microbial oil, particularly a kind of oleaginous microorganism that utilizes transforms the method that the carbon source that contains glucose and wood sugar prepares microbial oil.
Background technology
Microbial oil claims Unicell Oils and Fats again, is by microorganisms such as yeast, mould, bacterium and algae under certain conditions, and carbohydrate is converted into oil and fat accumulation in cell.Every microorganism that can surpass dry cell weight 20wt% at the intracellular accumulation grease is called oleaginous microorganism.It is reported that part microbial accumulation grease reaches (Li Yonghong, the Liu Bo more than 70% of dry cell weight, Zhao Zongbao, Bai Fengwu. red winter spore saccharomycetes to make fermentation produce oil fat substratum of circle and Optimizing Conditions of Fermentation research. biotechnology journal, 2006,22 (4): 650-656).Compare with oilseed plant production, the microbial transformation carbohydrate is produced grease, is not subjected to the influence of place, season and climate change, can realize grease mass-producing continuous production.The biofuel that biofuel that microbial oil makes through transesterificationization and animal-plant oil make is formed similar, so the microbial oil technology can be the bioenergy industry development new raw material is provided, and has the important application prospect.
Ligno-cellulosic materials is with low cost, wide material sources, total resources abundant, and its Chemical Composition mainly is Mierocrystalline cellulose, hemicellulose and xylogen.The cellulose hydrolysis primary product is a glucose; Hydrolysis of hemicellulose obtains mixtures such as glucose, wood sugar, semi-lactosi, pectinose, seminose.If can be that carbon source is cultivated oleaginous microorganism, will significantly reduce the microbial oil production cost with the ligno-cellulose hydrolysate using.
When microorganism utilizes the mixture of hexose and pentose, most microorganisms preferentially utilize glucose, treat that glucose just begins to utilize other carbon source when exhausting or being in very lower concentration, this phenomenon is called the carbon source metabolism and checks, or title glucose effect (Goerke B, Stulke J.Carbon catabolite repressionin bacteria:many ways to make the most out of nutrients.Nat Rev Microbiol, 2008,6 (8): 613-624; Nichols N N, Dien B S, Bothast R J.Use of cataboliterepression mutants for fermentation of sugar mixtures to ethanol.ApplMicrobiol Biotechnol, 2001,56 (1-2): 120-125).According to the literature, this Da Shi saccharomyces oleaginosus of oleaginous microorganism Lipomyces starkeyi (Kong Xiangli, Liu Bo, Zhao Zongbao, Feng Bin. this Da Shi saccharomyces oleaginosus utilizes mixing sugar fermentation produce oil fat. biological processing, 2007,5 (2): 36-41), fermentable trichosporon Trichosporon fermentans (Huang C, Zong M, Wu H, Liu Q.Microbialoil production from rice straw hydrolysate by Trichosporon fermentans.Bioresour Technol, 2009,100 (19): 4535-4538) wait when utilizing the mixture of glucose and wood sugar, preferentially utilize glucose, then just begin to utilize wood sugar.This can make the generative process of microorganism growth and product lag phase occur, i.e. diauxie, and this phenomenon causes that fermentation period prolongs, efficient reduces.Therefore searching can be synchronously and is efficiently utilized the microorganism of hexose and pentose significant for improving fermentation efficiency.
Remove the existing bibliographical information of correlative study that the carbon source metabolism is checked.In the process of continuously fermenting, glucose concn is maintained a lower level, and under a more suitable dilution rate, realized synchronous utilization (the Kastner J of glucose and wood sugar, Jones W, Roberts R.Simultaneousutilization of glucose and D-xylose by Candida shehatae in a chemostat.J IndMicrobiol Biot, 1998,20 (6): 339-343).Secondly, produce the needed enzyme of xylose metabolism by inducing early stage, also can test synchronous utilization (the Kastner J of mixing sugar, Roberts R.Simultaneousfermentation of D-xylose and glucose by Candida shehatae.Biotechnol Lett, 1990,12 (1): 57-60).In addition, by screening glucose phosphotransferase mutant strain, also can remove the glucose restraining effect, realize that glucose and wood sugar utilize synchronously, eliminate diauxie (Dien B, Nichols N, Bothast R.Fermentation of sugar mixtures using Escherichia colicatabolite repression mutants engineered for production of L-lactic acid.J IndMicrobiol Biot, 2002,29 (5): 221-227).
Summary of the invention
In carbon source, contain glucose and wood sugar, most of oleaginous microorganism preferentially utilizes glucose, have only when glucose completely consumed or concentration drop to and just begin to utilize wood sugar after very low, be called glucose and suppress, its direct result is exactly diauxie to occur in microorganism growth and the product cumulative process.The diauxie of oil fermentation process causes fermentation period prolongation, efficient reduction, production cost to improve.The contriver thinks that the diauxie of removing produce oil can significantly promote cheap carbon source effectively to utilize, and improves the Technological Economy of microbial oil.Therefore, the contriver utilizes a large amount of oleaginous microorganisms to carry out scientific experiment, finds that part oleaginous microorganism bacterial strain has the physiological property of synchronous consumption glucose and wood sugar.
The object of the present invention is to provide the carbon source that a kind of synchronous utilization contains glucose and wood sugar to cultivate oleaginous microorganism, produce the method for the microbial oil that contains one or more lipid acid and derivative thereof.The carbon source that technology of the present invention is easy, easy to implement the method, could utilize and transform the composition complexity more efficiently as the lignocellulosic material hydrolysate, is improved the Technological Economy that microbial oil is produced.
The present invention is achieved by following technical proposals:
1, preparation produce oil substratum.Realize by a kind of of following method or their necessary combined method:
A. glucose and wood sugar are mixed, make total sugar concentration and be 2%～10% the aqueous solution, add other necessary nutrition, pH 4.0～8.0, and the sterilization back is standby;
B. the reference literature method is with the lignocellulosic material hydrolysis, and product is made the aqueous solution that contains glucose and wood sugar, adds other necessary nutrition, and pH 4.0～8.0, and the sterilization back is standby; Or,
C. the organism-based raw material of reference literature method hydrolysis carbohydrate containing adds glucose or wood sugar and other necessary nutrition in the product, and pH 4.0～8.0, and the sterilization back is standby.
Other necessary nutrition is: conventional be used for the material that oleaginous microorganism is cultivated, as yeast powder etc.
2) oleaginous microorganism is cultivated.To the described inoculation of medium oleaginous microorganism of step (1) seed liquor, inoculum size 2%～20% (v/v), at 25 ℃～37 ℃ following aerated culture, residual sugar concentration is lower than 1% to the mash.
3) microorganism collection and microbial oil extract.After cultivate finishing, adopts centrifugal, filter or other necessary method concentrates collection oleaginous microorganism cell.Reference literature (Li Zhifeng, Zhang Ling, Shen Xiaojing, etc. the comparative studies of four kinds of fungal oil extracting method. the microbiology circular, 2001,28 (6), 72-75) use acid heat-organic solvent method extracting to obtain grease, calculate the thalline fat content.
Described microbial oil can further be processed into diesel-fuel or mix the mixture of use with diesel-fuel.
The oleaginous microorganism that the present invention uses can surpass dry cell weight 20% (w/w) for thalline fat content after fermentation culture, and fungi, bacterium or little algae with physiological property of synchronous consumption glucose and wood sugar.They comprise skin shape trichosporon Trichosporon cutaneum, crooked Cryptococcus Cryptococcus curvatus and red winter spore Rhodosporidium toruloides.These bacterial strains can be directly from comprising that Chinese common micro-organisms culture presevation administrative center (CGMCC) and American type culture collection culture presevation mechanisms such as (ATCC) buy or from the occurring in nature separation, also can using the artificial or spontaneous mutation bacterial strain different with original bacterial strain proterties.
The present invention adopts glucose and wood sugar blended method, hydrolysis is contained the method for glucose unit or wood sugar unit organism-based raw material or the combined method of necessity, and preparation contains the carbon source of glucose and wood sugar.Organism-based raw material hydrolysis reference literature method (Cara C, Ruiz E, Oliva J M, Saez F, Castro E.Conversion of olive tree biomass into fermentable sugars by diluteacid pretreatment and enzymatic saccharification.Bioresour Technol, 2008,99 (6): 1869-1876) carry out.Lignocellulosic material is the material that contains Mierocrystalline cellulose, hemicellulose and xylogen, comprises plant materials, agricultural crop straw and forestry processing waste, for example maize straw, straw, straw, pine, dragon spruce etc.
The invention has the beneficial effects as follows: 1) compare with traditional method by preparing biological diesel oil by animal plant lipid, the technology of the present invention is simply effective, and the cycle is short, saves and ploughs; 2) microorganism utilizes glucose and wood sugar synchronously, has overcome glucose to the inhibition that other monose utilizes, and has avoided diauxie in the process, has improved the efficient of fermenting process; 3) microorganism utilizes glucose and wood sugar accumulation microbial oil, provides convenience for efficient conversion lignocellulosic material prepares microbial oil.
Description of drawings
Base consumption and biomass formation curve among Fig. 1 embodiment 1, wherein, ■ glucose; ◆ wood sugar; ▲ biomass.
Embodiment
Be the specific embodiment for preparing microbial oil by mixing sugar below, can recognize the influence that different technology conditions generates microbial oil by embodiment.
Embodiment 1
1) glucose and wood sugar are mixed with mixing sugar solution, glucose 47g/L, wood sugar 23g/L, add the 1.0g/L yeast powder, 0.1g/L ammonium chloride, 1.0g/L magnesium chloride, 0.1g/L sodium sulfate, 11.8g/L potassium primary phosphate, 3.7g/L dipotassium hydrogen phosphate, trace element solution 1% (v/v), surplus is a water, pH 6.0, and is standby behind 121 ℃ of sterilizations of gained substratum 15min;
2) oleaginous yeast Trichosporon cutaneum AS 2.571 (available from Chinese common micro-organisms culture presevation administrative center) is in liquid seed culture medium, in 30 ℃, and 200 rev/mins of shaking culture 20 hours;
3) insert the oleaginous microorganism seed liquor that step (2) prepares in the described substratum of step (1), inoculum size 10% (v/v) is at 30 ℃ of following aerated culture 120h;
4) stop fermentation, remaining glucose and xylose concentration are respectively 0.1g/L, 1.9g/L in the fermented liquid at this moment; Solid-liquid separation is collected thalline and is washed twice with physiological saline, final dry mycelium 23.8g/L, the fat content 49.7% of getting.
Embodiment 2
1) glucose and wood sugar are mixed with mixing sugar solution, glucose 10g/L, wood sugar 10g/L adds the 2g/L yeast powder, 0.5g/L ammonium chloride, 0.2g/L magnesium chloride, 0.1g/L sodium sulfate, 0.4g/L potassium primary phosphate, 0.1g/L dipotassium hydrogen phosphate, trace element solution 1% (v/v), pH 5.0, and is standby behind 121 ℃ of sterilizations of gained substratum 15min;
2) oleaginous yeast Rhodosporidium toruloides AS 2.1389 (available from Chinese common micro-organisms culture presevation administrative center) is in liquid seed culture medium, in 30 ℃, and 200 rev/mins of shaking culture 18 hours;
3) insert the oleaginous microorganism seed liquor that step (2) prepares in the described substratum of step (1), inoculum size 2% (v/v) is at 25 ℃ of following aerated culture 60h;
4) stop fermentation, remaining glucose and xylose concentration are respectively 0.1g/L, 1.2g/L in the fermented liquid at this moment; Solid-liquid separation is collected thalline and with physiological saline washing twice, dry dry mycelium 5.1g/L; Obtain fat content 18% through organic solvent extraction.
Embodiment 3
1) glucose and wood sugar are mixed with mixing sugar solution, glucose 10g/L, wood sugar 50g/L adds the 0.7g/L yeast powder, 0.2g/L ammonium chloride, 1.1g/L magnesium chloride, 0.5g/L sodium sulfate, 8g/L potassium primary phosphate, the 2g/L dipotassium hydrogen phosphate, trace element solution 1% (v/v), pH 6.5, and is standby behind 121 ℃ of sterilizations of gained substratum 15min;
2) oleaginous yeast Cryptococcus curvatus ATCC 20509 (available from American type culture collection) is in liquid seed culture medium, in 30 ℃, and 200 rev/mins of shaking culture 24 hours;
3) insert the oleaginous microorganism seed liquor that step (2) prepares in the described substratum of step (1), inoculum size 5% (v/v) is at 28 ℃ of following aerated culture 144h;
4) stop fermentation, remaining glucose and xylose concentration are respectively 0g/L, 6.6g/L in the fermented liquid at this moment; Method according to embodiment 1 is carried out aftertreatment, final dry mycelium 15.7g/L, the fat content 43.2% of getting.
Embodiment 4
1) glucose and wood sugar are mixed with mixing sugar solution, glucose 30g/L, wood sugar 60g/L adds the 0.3g/L yeast powder, 0.5g/L ammonium chloride, 1.2g/L magnesium chloride, 0.3g/L sodium sulfate, 12g/L potassium primary phosphate, the 4g/L dipotassium hydrogen phosphate, trace element solution 1% (v/v), pH 7.0, and is standby behind 121 ℃ of sterilizations of gained substratum 15min;
2) oleaginous yeast Cryptococcus curvatus ATCC 20509 (available from American type culture collection) is in liquid seed culture medium, in 30 ℃, and 200 rev/mins of shaking culture 22 hours;
3) insert the oleaginous microorganism seed liquor that step (2) prepares in the described substratum of step (1), inoculum size 15% (v/v) is at 32 ℃ of following aerated culture 144h;
4) stop fermentation, remaining glucose and xylose concentration are respectively 0.2g/L, 2.5g/L in the fermented liquid at this moment; Method according to embodiment 1 is carried out aftertreatment, final dry mycelium 26.9g/L, the fat content 48.6% of getting.
Embodiment 5
1) glucose and wood sugar are mixed with mixing sugar solution, glucose 95g/L, wood sugar 5g/L adds the 1.2g/L yeast powder, 1.0g/L ammonium chloride, 0.6g/L magnesium chloride, 0.4g/L sodium sulfate, 10g/L potassium primary phosphate, the 6g/L dipotassium hydrogen phosphate, trace element solution 1% (v/v), pH 5.5, and is standby behind 121 ℃ of sterilizations of gained substratum 15min;
2) oleaginous yeast Cryptococcus curvatus ATCC 20509 (available from American type culture collection) is in liquid seed culture medium, in 30 ℃, and 200 rev/mins of shaking culture 28 hours;
3) insert the oleaginous microorganism seed liquor that step (2) prepares in the described substratum of step (1), inoculum size 20% (v/v) is at 34 ℃ of following aerated culture 168h;
4) stop fermentation, remaining glucose and xylose concentration are respectively 7.2g/L, 0g/L in the fermented liquid at this moment; Method according to embodiment 1 is carried out aftertreatment, final dry mycelium 24.3g/L, the fat content 40.1% of getting.
Embodiment 6
1) glucose and wood sugar are mixed with mixing sugar solution, glucose 20g/L, wood sugar 10g/L adds the 0.2g/L yeast powder, 0.3g/L ammonium chloride, 0.8g/L magnesium chloride, 0.1g/L sodium sulfate, 6g/L potassium primary phosphate, the 1g/L dipotassium hydrogen phosphate, trace element solution 1% (v/v), pH 6.2, and is standby behind 121 ℃ of sterilizations of gained substratum 15min;
2) oleaginous yeast Trichosporon cutaneum AS 2.571 (available from Chinese common micro-organisms culture presevation administrative center) is in liquid seed culture medium, in 30 ℃, and 200 rev/mins of shaking culture 26 hours;
3) insert the oleaginous microorganism seed liquor that step (2) prepares in the described substratum of step (1), inoculum size 12% (v/v) is at 37 ℃ of following aerated culture 72h;
4) stop fermentation, remaining glucose and xylose concentration are respectively 0.5g/L, 2.1g/L in the fermented liquid at this moment; Method according to embodiment 1 is carried out aftertreatment, final dry mycelium 6.6g/L, the fat content 23.5% of getting.
Embodiment 7
Reference literature method (Kootstra A, Beeffink H, Scott E, Sanders J.Comparison ofdilute mineral and organic acid pretreatment for enzymatic hydrolysis of wheatstraw.Biochem Eng J, 2009,46 (2): 126-131), be raw material with the maize straw, dry, pulverized 60 mesh sieves and 50mM maleic acid solution and mix, solid-liquid ratio is 10% (w/w), soak 24h, 170 ℃ of digestion 30min, being diluted to solid-liquid ratio is 5% (w/w), transfers pH 4.8, the cellulase add-on is the 50FPU/g solid, 60 ℃, 150rpm reacts 72h, filters, and adjusting total reducing sugars concentration is 25g/L, glucose 17g/L wherein, wood sugar 8g/L.In this liquid glucose, add glucose 50g/L, the 1.0g/L yeast powder, 0.6g/L ammonium chloride, the 0.8g/L magnesium chloride, 0.1g/L sodium sulfate, the 5g/L potassium primary phosphate, the 2g/L dipotassium hydrogen phosphate, trace element solution 1% (v/v) is transferred pH 5.0.Behind 121 ℃ of sterilization 15min, insert Trichosporon cutaneum AS 2.571 (available from Chinese common micro-organisms culture presevation administrative center) seed liquor, in 35 ℃ of aerated culture 144 hours with 10% (v/v) inoculum size.Stop fermentation, remaining glucose and xylose concentration are respectively 2.1g/L and 0.2g/L in the fermented liquid at this moment.Method according to embodiment 1 is carried out aftertreatment, final dry mycelium 22.4g/L, the fat content 38.5% of getting.
Embodiment 8
Reference literature (Sassner P, Martensson C G, Galbe M, Zacchi G.Steampretreatment of H2SO4-impregnated Salix for the production of bioethanol.Bioresour Technol, 2008,99 (1): 137-145) method, with the willow is raw material, drying was pulverized 60 mesh sieves, soaked 90min in 0.5% excessive sulfuric acid; Filter, obtaining the feed liquid solids content is 40%, 200 ℃ of stop 8min, carries out steam explosion then; Filtering, is 2% with the solid matter thin up to solids content, adds cellulase 65FPU/g and glucuroide 393IU/g, 40 ℃ of reaction 96h; Filter, adjusting total reducing sugars concentration is 60g/L, glucose 45g/L wherein, wood sugar 15g/L.In this liquid glucose, add the 1.5g/L yeast powder, 1.0g/L ammonium chloride, the 0.5g/L magnesium chloride, 0.1g/L sodium sulfate, the 7.5g/L potassium primary phosphate, the 2.5g/L dipotassium hydrogen phosphate, trace element solution 1% (v/v) is transferred pH 6.0.Behind 121 ℃ of sterilization 15min, insert Trichosporoncutaneum AS 2.571 seed liquor, in 32 ℃ of aerated culture 100 hours with 15% (v/v) inoculum size.Stop fermentation, remaining glucose and xylose concentration are respectively 0.8g/L and 0.1g/L in the fermented liquid at this moment.Method according to embodiment 1 is carried out aftertreatment, final dry mycelium 19.6g/L, the fat content 33.5% of getting.
Embodiment 9
Reference literature (Sun Y, Cheng J J.Dilute acid pretreatment of rye straw andbermudagrass for ethanol production.Bioresour Technol, 2005,96 (14): 1599-1606) method is a raw material with the rye straw, drying, pulverized 60 mesh sieves, mix with 1.5% (w/w) dilute sulphuric acid, solid-liquid ratio is 10% (w/w), 121 ℃ of digestion 90min; Filter, add cellulase (25FPU/g solid) and glucuroide (75IU/g solid) in the solid matter, 50 ℃, 100rpm react 48h; Filter, adjusting total reducing sugars concentration is 90g/L, glucose 56g/L wherein, wood sugar 34g/L.In this liquid glucose, add the 1.2g/L yeast powder, 0.3g/L ammonium chloride, the 0.9g/L magnesium chloride, 0.1g/L sodium sulfate, the 6g/L potassium primary phosphate, the 3g/L dipotassium hydrogen phosphate, trace element solution 1% (v/v) is transferred pH 5.5.Behind 121 ℃ of sterilization 15min, insert Cryptococcuscurvatus ATCC 20509 (available from American type culture collection) seed liquor, in 28 ℃ of aerated culture 132 hours with 20% (v/v) inoculum size.Stop fermentation, remaining glucose and xylose concentration are respectively 3.1g/L and 1.6g/L in the fermented liquid at this moment.Method according to embodiment 1 is carried out aftertreatment, final dry mycelium 23.8g/L, the fat content 39.4% of getting.
Compare with the fermenting process that preferentially utilizes glucose of routine, the present invention relates to the synchronous utilization of glucose and wood sugar, can eliminate the diauxie in the fermenting process, improve substrate conversion speed, shorten fermentation period, thereby reduce production costs; Compare with the method for other feedstock production microbial oils of bio-transformation, the raw material that the present invention uses is the lignocellulose biomass that contains glucose and wood sugar, as trees, stalk etc., have aboundresources, wide material sources, cheap advantage, and fermentation obtain greasy easy to implement the method, cost is low, is a kind of new way of utilizing renewable resources to produce microbial oil.
Claims (5)
1. the preparation method of a microbial oil is characterized in that: adopt the carbon source that contains glucose and wood sugar to cultivate oleaginous microorganism, obtain to contain the grease of one or more lipid acid and derivative thereof, glucose and wood sugar are by the oleaginous microorganism synchronous consumption;
Glucose content is 5%～95% in the described carbon source;
Described oleaginous microorganism is one or more among skin shape trichosporon Trichosporon cutaneum, crooked Cryptococcus Cryptococcus curvatus, the red winter spore Rhodosporidium toruloides.
2. according to the preparation method of the described microbial oil of claim 1, it is characterized in that:
Described oleaginous microorganism is cultivated and carry out 20 ℃～37 ℃ of culture temperature, pH4.0～8.0 under aerobic conditions.
3. according to the preparation method of the described microbial oil of claim 1, it is characterized in that: the described carbon source that contains glucose and wood sugar is that perhaps lignocellulosic material to be hydrolyzed to handle make by glucose and wood sugar is mixed.
4. according to the preparation method of the described microbial oil of claim 3, it is characterized in that: described lignocellulosic material is the biomaterial that contains Mierocrystalline cellulose, hemicellulose and xylogen.
5. according to the preparation method of the described microbial oil of claim 3, it is characterized in that: described lignocellulosic material is agricultural crop straw or forestry processing waste.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103525537A (en) * | 2013-10-22 | 2014-01-22 | 嘉必优生物工程（武汉）有限公司 | Method of extracting microbial oil |
| CN105087688A (en) * | 2014-05-07 | 2015-11-25 | 中国科学院大连化学物理研究所 | Production method of microbial oil |
| CN105693043A (en) * | 2016-04-22 | 2016-06-22 | 东北电力大学 | Soybean oil wastewater treatment and resource utilization method |
| CN106318985A (en) * | 2016-08-24 | 2017-01-11 | 嘉必优生物技术（武汉）股份有限公司 | Microbial lipid |
| CN106636234A (en) * | 2015-10-29 | 2017-05-10 | 清华大学 | Method for producing microbial oil through combination of bacterial flora and oleaginous microorganisms |
| CN107075537A (en) * | 2014-08-01 | 2017-08-18 | 国立研究开发法人科学技术振兴机构 | Produce the yeast and grease manufacture method of grease |
| CN107201385A (en) * | 2016-03-17 | 2017-09-26 | 金丹凤 | A kind of utilization rice straw produces the new method of biodiesel |
| CN107557400A (en) * | 2017-10-12 | 2018-01-09 | 东北电力大学 | A kind of method for improving corn stalk hydrolysis culture saccharomyces oleaginosus oil production |
| CN109666708A (en) * | 2018-12-24 | 2019-04-23 | 合肥工业大学 | It is a kind of that linoleic method being produced by wood-sugar fermentation using basket bacterium |
| CN117660189A (en) * | 2024-02-01 | 2024-03-08 | 杭州楠大环保科技有限公司 | Method for producing grease by microalgae mixed culture |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101343644A (en) * | 2007-07-13 | 2009-01-14 | 中国科学院大连化学物理研究所 | Method for preparing microorganism oil and fat with corn stalk as raw material |
-
2010
- 2010-05-19 CN CN201010176591XA patent/CN102250974A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101343644A (en) * | 2007-07-13 | 2009-01-14 | 中国科学院大连化学物理研究所 | Method for preparing microorganism oil and fat with corn stalk as raw material |
Non-Patent Citations (3)
| Title |
|---|
| 张杰等: "多纤维素酶协同降解玉米秸秆及水解液微生物油脂发酵研究", 《现代化工》 * |
| 张继泉 等: "玉米秸秆稀硫酸预处理条件的初步研究", 《纤维素科学与技术》 * |
| 李永红 等: "广谱碳源产油酵母菌的筛选", 《中国生物工程杂志》 * |
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| CN103525537A (en) * | 2013-10-22 | 2014-01-22 | 嘉必优生物工程（武汉）有限公司 | Method of extracting microbial oil |
| CN105087688A (en) * | 2014-05-07 | 2015-11-25 | 中国科学院大连化学物理研究所 | Production method of microbial oil |
| CN107075537A (en) * | 2014-08-01 | 2017-08-18 | 国立研究开发法人科学技术振兴机构 | Produce the yeast and grease manufacture method of grease |
| CN106636234B (en) * | 2015-10-29 | 2021-11-16 | 清华大学 | Method for producing microbial oil by combining flora and oil-producing microorganisms |
| CN106636234A (en) * | 2015-10-29 | 2017-05-10 | 清华大学 | Method for producing microbial oil through combination of bacterial flora and oleaginous microorganisms |
| CN107201385A (en) * | 2016-03-17 | 2017-09-26 | 金丹凤 | A kind of utilization rice straw produces the new method of biodiesel |
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| CN106318985A (en) * | 2016-08-24 | 2017-01-11 | 嘉必优生物技术（武汉）股份有限公司 | Microbial lipid |
| CN107557400A (en) * | 2017-10-12 | 2018-01-09 | 东北电力大学 | A kind of method for improving corn stalk hydrolysis culture saccharomyces oleaginosus oil production |
| CN109666708A (en) * | 2018-12-24 | 2019-04-23 | 合肥工业大学 | It is a kind of that linoleic method being produced by wood-sugar fermentation using basket bacterium |
| CN117660189A (en) * | 2024-02-01 | 2024-03-08 | 杭州楠大环保科技有限公司 | Method for producing grease by microalgae mixed culture |
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