CN107201385A - A kind of utilization rice straw produces the new method of biodiesel - Google Patents
A kind of utilization rice straw produces the new method of biodiesel Download PDFInfo
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- CN107201385A CN107201385A CN201610150563.8A CN201610150563A CN107201385A CN 107201385 A CN107201385 A CN 107201385A CN 201610150563 A CN201610150563 A CN 201610150563A CN 107201385 A CN107201385 A CN 107201385A
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- rice straw
- biodiesel
- hydrolyzate
- fermentation
- new method
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses the new method that a kind of utilization rice straw produces biodiesel, comprise the following steps：Trichoderma, aspergillus oryzae and Phanerochaete chrysosporium carry out solid state fermentation with 90: 8: 2 ratio mixing, 28 30 DEG C, the static fermentation of wet seal enclosed 4 days is not controlled, rice straw is converted into various small molecule carbohydrates；Then it is that fermenting raw materials produce polyunsaturated fatty acid in the above-mentioned stalk hydrolyzate of 25 28 DEG C of utilizations by Trichosporon fermentans；Finally using the Escherichia coli homologous recombination bacterium with fatty-acid ethyl ester (i.e. biodiesel) synthesis capability built, it is that liquid glucose and unrighted acid are raw material progress biodiesel preparation to use the rice straw hydrolyzate of above-mentioned preparation.Gas chromatographic analysis shows that the biodiesel main component that fermentation is obtained is ethyl oleate, ethyl myristate, ethyl palmitate and hexadecene acetoacetic ester.The biodiesel indices that production is obtained meet international bio diesel oil standard.
Description
Technical field
The new side of biodiesel is produced the present invention relates to new energy field, more particularly to a kind of utilization rice straw
Method.
Background technology
The energy is the basis that all life are present, and current we live the consumed energy mainly fossil energy,
But the exploitation of fossil energy is utilized and is just a makeshift arrangement.The energy such as China's oil, coal, power supply shortage
Source problem is increasingly shown especially, and energy shortage has become the bottleneck of restriction China economic development.Therefore, develop
The future development of economically feasible, environment-friendly novel energy to the mankind is extremely urgent.
Preparing biodiesel using more esterification process at present not only needs large-scale plantation oil crops, also
Oil crops are carried out in various physical chemistry processing, course of reaction using excessive methanol and free fatty
Biodiesel production rate and quality can be had a strong impact on, salkali waste, acid solution discharge easily cause secondary pollution to environment.
Moreover China be not oil crops plantation big country, and have a large population in a small area, anxiety of plough, it is impossible to large area plantation
Oil crops.
The content of the invention
The present invention proposes the new method that a kind of utilization rice straw produces biodiesel, and the present invention utilizes the modern times
Discarded rice straw is converted into biodiesel by biotechnology, acts not only as new resources, new energy, together
When the problem of can also alleviate crop genetic resource environmental pollution.
A kind of utilization rice straw proposed by the present invention produces the new method of biodiesel, comprises the following steps：
S1, trichoderma, aspergillus oryzae and Phanerochaete chrysosporium are mixed with 90: 8: 2 ratio, with rice straw
For substrate, wet, closed static fermentation 4 days is not controlled, rice straw hydrolyzate is obtained by 28-30 DEG C；
S2, using rice straw hydrolyzate as substrate, utilize Trichosporon fermentans carry out liquid state fermentation, 25-28 DEG C
Fermentation 8 days, pH is 6.5, obtains the microbial grease using polyunsaturated fatty acid as composition.
S3, using the Escherichia coli homologous recombination bacterium with fatty-acid ethyl ester synthesis capability of structure as fermentation strain,
It is that fatty acid source enters for the obtained microbial grease of liquid glucose and S2 to use rice straw hydrolyzate prepared by S1
It is prepared by row biodiesel.Material composition is rice straw hydrolyzate (sugar amount) 4%, microbial grease 1%, egg
White peptone 1%, yeast extract 0.5%, sodium chloride 1%, pH is 7.0.After being cultivated 5 hours at 37 DEG C, plus
Enter 0.1mM IPTG, stuffiness, mixing speed is 100r/min, fermented and cultured 48 hours.
Preferably, in S1, trichoderma, aspergillus oryzae and Phanerochaete chrysosporium are mixed with 90: 8: 2 ratio,
Using rice straw as substrate, rice straw hydrolyzate is obtained.
Preferably, in S2, using rice straw hydrolyzate as substrate, liquid hair is carried out using Trichosporon fermentans
Ferment, obtains microbial grease.
Preferably, in S3, the microbial grease obtained using rice straw hydrolyzate as liquid glucose and fermentation is fat
Acid source carries out biodiesel preparation.
In the present invention, biodiesel is produced by raw material of the agriculture waste stalk of China's abundant, cost of material is big
Big reduction；Zymolysis Equipment is less demanding, it is not necessary to which catalyst, production cost is low；It is not required in process of production
Vegetable fat is directly fed, cultivated land resource is not take up；Without using methanol and some chemical catalysts, no
Environment can be polluted, will not also cause potential hazard to producers.Biodiesel indices meet
International bio diesel oil standard.
Embodiment
Below, technical scheme is described in detail by specific embodiment.
Embodiment 1
A kind of utilization rice straw produces the new method of biodiesel, comprises the following steps：
S1, trichoderma, aspergillus oryzae and Phanerochaete chrysosporium are mixed with 90: 8: 2 ratio, with rice straw
For substrate, wet, closed static fermentation 4 days is not controlled, rice straw hydrolyzate is obtained by 28 DEG C；
S2, using rice straw hydrolyzate as substrate, utilize Trichosporon fermentans carry out liquid state fermentation, 25 DEG C hair
Ferment 8 days, pH is 6.5, obtains the microbial grease using polyunsaturated fatty acid as composition.
S3, using the Escherichia coli homologous recombination bacterium with fatty-acid ethyl ester synthesis capability of structure as fermentation strain,
It is that fatty acid source enters for the obtained microbial grease of liquid glucose and S2 to use rice straw hydrolyzate prepared by S1
It is prepared by row biodiesel.Material composition is rice straw hydrolyzate (sugar amount) 4%, microbial grease 1%, egg
White peptone 1%, yeast extract 0.5%, sodium chloride 1%, pH is 7.0.After being cultivated 5 hours at 37 DEG C, plus
Enter 0.1mM IPTG, stuffiness, mixing speed is 100r/min, fermented and cultured 48 hours.
Embodiment 2
A kind of utilization rice straw produces the new method of biodiesel, comprises the following steps：
S1, trichoderma, aspergillus oryzae and Phanerochaete chrysosporium are mixed with 90: 8: 2 ratio, with rice straw
For substrate, wet, closed static fermentation 4 days is not controlled, rice straw hydrolyzate is obtained by 30 DEG C；
S2, using rice straw hydrolyzate as substrate, utilize Trichosporon fermentans carry out liquid state fermentation, 26 DEG C hair
Ferment 8 days, pH is 6.5, obtains the microbial grease using polyunsaturated fatty acid as composition.
S3, using the Escherichia coli homologous recombination bacterium with fatty-acid ethyl ester synthesis capability of structure as fermentation strain,
It is that fatty acid source enters for the obtained microbial grease of liquid glucose and S2 to use rice straw hydrolyzate prepared by S1
It is prepared by row biodiesel.Material composition is rice straw hydrolyzate (sugar amount) 4%, microbial grease 1%, egg
White peptone 1%, yeast extract 0.5%, sodium chloride 1%, pH is 7.0.After being cultivated 5 hours at 37 DEG C, plus
Enter 0.1mM IPTG, stuffiness, mixing speed is 100r/min, fermented and cultured 48 hours.
Embodiment 3
A kind of utilization rice straw produces the new method of biodiesel, comprises the following steps：
S1, trichoderma, aspergillus oryzae and Phanerochaete chrysosporium are mixed with 90: 8: 2 ratio, with rice straw
For substrate, wet, closed static fermentation 4 days is not controlled, rice straw hydrolyzate is obtained by 29 DEG C；
S2, using rice straw hydrolyzate as substrate, utilize Trichosporon fermentans carry out liquid state fermentation, 28 DEG C hair
Ferment 8 days, pH is 6.5, obtains the microbial grease using polyunsaturated fatty acid as composition.
S3, using the Escherichia coli homologous recombination bacterium with fatty-acid ethyl ester synthesis capability of structure as fermentation strain,
It is that fatty acid source enters for the obtained microbial grease of liquid glucose and S2 to use rice straw hydrolyzate prepared by S1
It is prepared by row biodiesel.Material composition is rice straw hydrolyzate (sugar amount) 4%, microbial grease 1%, egg
White peptone 1%, yeast extract 0.5%, sodium chloride 1%, pH is 7.0.After being cultivated 5 hours at 37 DEG C, plus
Enter 0.1mM IPTG, stuffiness, mixing speed is 100r/min, fermented and cultured 48 hours.
Treatment effect：The small molecular sugar conversion ratio of mixed culture solid state fermentation rice straw production can reach 85%；
Trichosporon fermentans carry out liquid deep layer fermenting, maximum fat content (many insatiable hungers using rice straw hydrolyzate
65% or so is accounted for fatty acid component) 44.8% is can reach, grease yield is up to 12.8g/L, carbon source
Conversion ratio is 68.4%；The yield of homologous recombination bacterium production biodiesel is 2.81g/L, and grease conversion is made a living
The conversion ratio of thing diesel oil is 28.1%, and carbon source conversion ratio reaches 75%.The biodiesel main component of production is oil
Acetoacetic ester, ethyl myristate, ethyl palmitate and hexadecene acetoacetic ester, indices meet international life
Thing diesel oil standard.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention not office
Be limited to this, any one skilled in the art the invention discloses technical scope in, according to this
The technical scheme of invention and its inventive concept are subject to equivalent substitution or change, should all cover the protection in the present invention
Within the scope of.
Claims (4)
1. a kind of utilization rice straw produces the new method of biodiesel, it is characterised in that comprise the following steps：
S1, trichoderma, aspergillus oryzae and Phanerochaete chrysosporium are mixed with 90: 8: 2 ratio, with rice straw
For substrate, wet, closed static fermentation 4 days is not controlled, rice straw hydrolyzate is obtained by 28-30 DEG C；
S2, using rice straw hydrolyzate as substrate, utilize Trichosporon fermentans carry out liquid state fermentation, 25-28 DEG C
Fermentation 8 days, pH is 6.5, obtains the microbial grease using polyunsaturated fatty acid as composition.
S3, using the Escherichia coli homologous recombination bacterium with fatty-acid ethyl ester synthesis capability of structure as fermentation strain,
It is that fatty acid source enters for the obtained microbial grease of liquid glucose and S2 to use rice straw hydrolyzate prepared by S1
It is prepared by row biodiesel.Material composition is rice straw hydrolyzate (sugar amount) 4%, microbial grease 1%, egg
White peptone 1%, yeast extract 0.5%, sodium chloride 1%, pH is 7.0.After being cultivated 5 hours at 37 DEG C, plus
Enter 0.1mM IPTG, stuffiness, mixing speed is 100r/min, fermented and cultured 48 hours.
2. rice straw according to claim 1 produces the new method of biodiesel, it is characterised in that
In S1, trichoderma, aspergillus oryzae and Phanerochaete chrysosporium are mixed with 90: 8: 2 ratio, with rice straw
For substrate, rice straw hydrolyzate is obtained.
3. rice straw according to claim 1 produces the new method of biodiesel, it is characterised in that
In S2, using rice straw hydrolyzate as substrate, liquid state fermentation is carried out using Trichosporon fermentans, micro- life is obtained
Thing grease.
4. rice straw according to claim 1 produces the new method of biodiesel, it is characterised in that
In S3, it is aliphatic acid for the obtained microbial grease of liquid glucose and S2 to use rice straw hydrolyzate prepared by S1
Source carries out biodiesel preparation.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610150563.8A CN107201385A (en) | 2016-03-17 | 2016-03-17 | A kind of utilization rice straw produces the new method of biodiesel |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610150563.8A CN107201385A (en) | 2016-03-17 | 2016-03-17 | A kind of utilization rice straw produces the new method of biodiesel |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108277195A (en) * | 2018-04-28 | 2018-07-13 | 河南师范大学 | The method for generating chlamydospore using Phanerochaete chrysosporium stationary solid fermentation agricultural wastes and accumulating grease |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250974A (en) * | 2010-05-19 | 2011-11-23 | 中国科学院大连化学物理研究所 | Preparation method of microbial oil |
| CN102286572A (en) * | 2011-06-23 | 2011-12-21 | 浙江大学 | Method for preparing fermentable sugar solution from straws |
-
2016
- 2016-03-17 CN CN201610150563.8A patent/CN107201385A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250974A (en) * | 2010-05-19 | 2011-11-23 | 中国科学院大连化学物理研究所 | Preparation method of microbial oil |
| CN102286572A (en) * | 2011-06-23 | 2011-12-21 | 浙江大学 | Method for preparing fermentable sugar solution from straws |
Non-Patent Citations (1)
| Title |
|---|
| 王兵: "基因工程菌E.coli pET28a(+)-PAW的构建及其转化废弃物产生物柴油的研究", 《万方数据》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108277195A (en) * | 2018-04-28 | 2018-07-13 | 河南师范大学 | The method for generating chlamydospore using Phanerochaete chrysosporium stationary solid fermentation agricultural wastes and accumulating grease |
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Application publication date: 20170926 |