CN106929543A - A kind of method of utilization multiple bacteria compound fermentation fuel ethanol produced by straw - Google Patents
A kind of method of utilization multiple bacteria compound fermentation fuel ethanol produced by straw Download PDFInfo
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- CN106929543A CN106929543A CN201511021364.9A CN201511021364A CN106929543A CN 106929543 A CN106929543 A CN 106929543A CN 201511021364 A CN201511021364 A CN 201511021364A CN 106929543 A CN106929543 A CN 106929543A
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- biomass
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- bacterium
- seed liquor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
A kind of method for producing alcohol fuel, comprises the following steps：1) using mixed liquor or biomass (such as maize straw, the wheat stalk or rice straw) hydrolysate of glucose and xylose as carbon source, dusty yeast and trace element are added, obtains fermentation medium；2) by the seed liquor of the seed liquor of glucose fermentation bacterium and wood-sugar fermentation bacterium according to 1:1~1:The ratio of 3 (v/v), while being inoculated in fermentation medium, ferments 36~60 hours at a temperature of 25~38 DEG C.Glucose Efficient Conversion can be ethanol by the method, and xylose is farthest changed into ethanol simultaneously, and the yield of by-product glycerin is significantly reduced in zymotic fluid, significantly improves extraction and purification step, and the production cost of cellulosic ethanol is substantially reduced.
Description
Technical field
The invention belongs to Fermentation Engineering and biomass energy source domain, relate to the use of multiple bacteria compound fermentation
The method of fuel ethanol produced by straw, the method can be by the fiber such as stalk or the stalk of pretreatment
The glucose and xylose of matter feed degradation is converted into ethanol, realizes the thorough of glucose and xylose in stalk
Bottom utilizes.
Background technology
It is the focus of current energy field research with lignocellulosic as raw material production alcohol fuel.Wood
Matter cellulose substances are mainly made up of cellulose, hemicellulose and lignin.Lignocellulose raw material
Middle cellulose accounts for the 35~45% of dry weight, and hemicellulose accounts for 20~40%.Cellulosic hydrolysates
It is glucose；And hydrolysis of hemicellulose product is more complicated, mainly based on xylose and glucose
Monose.In all these hydrolysates, glucose and xylose content accounts for the overwhelming majority.Therefore, Portugal
The efficient abundant conversion of grape sugar and xylose is the key issue that cellulosic ethanol production must be solved.
Research shows, makes full use of the wood-sugar fermentation in lignocellulose raw material to generate ethanol, can make wood
The yield of matter fibrous raw material alcohol fermentation increases by 25% on the original basis.Therefore, the ethanol of xylose
Whether fermentation is considered as the economically viable pass of lignocellulose raw material bioconversion production ethanol by people always
Key link.Nature only has a small number of bacteriums, saccharomycete and fungi can xylose fermentation ethanol, utilization
The metabolic process of microbial fermentation xylose synthesizing alcohol as shown in figure 1, there is by-product in these bacterial strains mostly
Thing (glycerine, xylitol etc.) many, ethanol yield is low, it is sensitive to low concentration mortifier in hydrolysate,
The low problem of alcohol resistance.Be traditionally used for alcohol fermentation production microorganism (saccharomyces cerevisiae and
Zymomonas mobilis) glucose, and alcohol fermentation efficiency high can be well utilized, ethanol is resistance to
It is strong by ability, but it is unable to fermenting xylose.Therefore, many researchers of recent domestic are devoted to
Structure can efficiently be metabolized the gene recombination bacterium of pentose (mainly xylose) and hexose producing and ethanol,
If the A of Chinese patent application CN 101942435 in saccharomyces cerevisiae by importing Candida boidinii
The fragment of the encoding gene of fermenting xylose relevant enzyme, enables saccharomyces cerevisiae secreting, expressing xylose isomerase
Enzyme, can effectively utilize xylose production ethanol.But the mistake of alcohol fermentation is carried out using gene recombination bacterium
Cheng Zhong, the problems such as there is also spawn degeneration.
Based on above present Research, the present invention propose it is a kind of based on multi-cultur es mixed culture (including
Glucose fermentation strain and xylose-fermenting strain) fermented stalk hydrolysate (predominantly glucose and
Xylose) the efficient method for producing alcohol fuel.The method substantially the glucose in stalk and
Xylose is ethanol, and significantly reduces the synthesis of by-product glycerin, is conducive to the high yield of ethanol
Produce and efficiently separate, the production cost of cellulosic ethanol is greatly lowered, to realize fibrous raw material
The industrialization for producing alcohol fuel provides technical foundation.
The content of the invention
It is an object of the invention to provide one kind based on multi-cultur es mixed culture (including glucose fermentation bacterium
Plant and Microorganisms of Fermenting Xylose) fermented stalk hydrolysate (predominantly glucose and xylose) efficiently life
The method for producing alcohol fuel, to make full use of stalk hydrolysate glucose and xylose to produce ethanol,
And reduce the synthesis of by-product glycerin, realize biomass it is efficient using and cellulosic ethanol production into
This effective reduction.
The first aspect of the present invention is related to a kind of method for producing alcohol fuel, comprises the following steps：
1) with mixed liquor or biomass (such as maize straw, Wheat Straw of glucose and xylose
Stalk or rice straw) used as carbon source, addition dusty yeast and trace element are fermented hydrolysate
Culture medium；
2) by the seed liquor of the seed liquor of glucose fermentation bacterium and wood-sugar fermentation bacterium according to 1:1~1:3
(v/v) ratio (such as 1:2), while being inoculated in fermentation medium, at 25~38 DEG C (for example
27 DEG C, 30 DEG C, 32 DEG C, 33 DEG C, 35 DEG C, 36 DEG C, 37 DEG C or 38 DEG C) at a temperature of ferment 36~60
Hour (such as 40 hours, 15 hours, 48 hours, 50 hours, 55 hours, 56 hours or
58 hours)；
Or,
The seed liquor of glucose fermentation bacterium is first inoculated in fermentation medium, at 25~38 DEG C (for example
25 DEG C, 28 DEG C, 35 DEG C or 38 DEG C) at a temperature of fermentation 24~36 hours (such as 28 hours, 30
Hour, 32 hours or 36 hours), the seed liquor of wood-sugar fermentation bacterium is inoculated, in 25~42 DEG C of (examples
Such as 25 DEG C, 28 DEG C, 38 DEG C or 42 DEG C) continue to ferment 12~36 hours (such as 20 hours, 25
Hour, 30 hours, 32 hours or 36 hours), the wherein seed liquor and wood of glucose fermentation bacterium
The zymogenic seed liquor of sugar presses 1:1~3:The ratio (such as 1 of 1 (v/v):2) it is inoculated with.
In a preferred embodiment, the production alcohol fuel described in first aspect present invention
Method, it is characterised in that concentration of glucose is 24g/L~180g/L in the mixed liquor of glucose and xylose,
Xylose concentration is 6g/L~45g/L.
In another preferred embodiment, the production alcohol fuel described in first aspect present invention
Method, it is characterised in that the preparation method of biomass hydrolysate is comprised the following steps：
The preparation method of described biomass hydrolysate is comprised the following steps：
With acid solution or aqueous slkali soaking biomass (such as maize straw, wheat stalk or paddy rice straw
Stalk) pre-processed, or by biomass (such as maize straw, wheat stalk or rice straw)
Access whiterot fungi (such as Phanerochaete chrysosporium (such as Phanerochaete chrysosporium),
Worm intends wax bacterium (Ceriporiopsis subvermispora) etc.) pre-processed, after pretreatment
Biomass material to adjust to pH be 6.0~7.0, drying adds water and is configured to concentration for 30~90g/L
The solution of (such as 30g/L, 40g/L, 50g/L, 60g/L, 70g/L, 80g/L), addition
Amount is respectively 0.1~15U/g biomass (such as 8U/g biomass, 10U/g biomass, 12U/g
Biomass, 15U/g biomass), hydrolysis temperature and enzymolysis pH value are adjusted, be hydrolyzed reaction,
Obtain biomass hydrolysate；
Or, the preparation method of the biomass hydrolysate is comprised the following steps：
Matter is added water be configured to concentration for 30~90g/L (such as 30g/L, 40g/L, 50g/L,
60g/L, 70g/L, 80g/L) solution, addition cellulase (such as Jie Neng sections produce Spezyme CP)
And/or zytase (such as Jie Neng sections produce Accellerase XC), addition is respectively 0.1~15U/g
(for example 8U/g biomass, 10U/g biomass, 12U/g biomass, 15U/g are biological for biomass
Matter), hydrolysis temperature and enzymolysis pH value are adjusted, be hydrolyzed reaction, obtains biomass by hydrolyzation product
Thing,
Or, the preparation method of described biomass hydrolysate is comprised the following steps：
With acid solution or aqueous slkali soaking biomass (such as maize straw, wheat stalk or paddy rice straw
Stalk) pre-processed, or by biomass (such as maize straw, wheat stalk or rice straw)
Access whiterot fungi (such as Phanerochaete chrysosporium (such as Phanerochaete chrysosporium),
Worm intends wax bacterium (Ceriporiopsis subvermispora) etc.) pre-processed, after pretreatment
Biomass material to adjust to pH be 6.0~7.0, drying adds water and is configured to concentration for 30~90g/L
The solution of (such as 30g/L, 40g/L, 50g/L, 60g/L, 70g/L, 80g/L), addition
(such as Jie Neng sections produce for cellulase (such as Jie Neng sections produce Spezyme CP) and/or zytase
Accellerase XC), addition be respectively 0.1~15U/g biomass (such as 8U/g biomass,
10U/g biomass, 12U/g biomass, 15U/g biomass), adjust hydrolysis temperature and enzymolysis
PH value, be hydrolyzed reaction, obtains biomass hydrolysate.
In another preferred embodiment, the production alcohol fuel described in first aspect present invention
Method, it is characterised in that described trace element be potassium dihydrogen phosphate and/or magnesium sulfate,
Preferably, the addition of potassium dihydrogen phosphate be 0.5~3g/L (such as 0.1g/L, 2g/L,
3g/L), the addition of magnesium sulfate be 0.1~1g/L (such as 0.2g/L, 0.5g/L, 0.6g/L,
1g/L)。
In another preferred embodiment, the method for production alcohol fuel of the present invention,
Characterized in that, the addition of dusty yeast be 0.3~3g/L (such as 0.5g/L, 0.8g/L, 1g/L,
2g/L)。
In another preferred embodiment, the production alcohol fuel described in first aspect present invention
Method, it is characterised in that it is following i) to viii) in one or more：
I) pre-process before, by biomass straw be crushed to particle diameter for 5~30mm (such as 10mm,
15mm、20mm、30mm)；
Ii) acid solution for 0.1~0.5mol/L (such as 0.2mol/L, 0.3mol/L) sulfuric acid or
Hydrochloric acid solution；
Iii) aqueous slkali is the NaOH solution or ammoniacal liquor of 0.5~1mol/L (such as 0.8mol/L)
Solution；
Iv it is) 12~24h with the time of acid solution or aqueous slkali soaking biomass；
V) after inoculation whiterot fungi, pretreatment time is 10~20 days；
Vi) hydrolysis temperature is 45~55 DEG C (such as 50 DEG C)；
Vii) enzymolysis pH value is 3.0~11.0 (such as 5.0,5.5,6.0,6.5,7.0,7.5,8.0
Or 8.5)；
Viii) enzymolysis time is 40~55 hours (such as 48 hours or 50 hours).
The second aspect of the present invention is related to the method for another production alcohol fuel, including following step
Suddenly：
1) acid solution or aqueous slkali soaking biomass (such as maize straw, wheat stalk or water are used
Rice straw) pre-processed, or by biomass material (such as maize straw, wheat stalk or
Rice straw) access whiterot fungi (such as Phanerochaete chrysosporium (such as Phanerochaete
Chrysosporium), worm intends wax bacterium (Ceriporiopsis subvermispora etc.) carries out pre- place
Reason, it is 6.0~7.0 that pretreated biomass material is adjusted to pH, and drying adds water and is configured to
Concentration is the solution of 30~90g/L, adds dusty yeast and trace element, obtains fermentation medium；
2) by the seed liquor of the seed liquor of glucose fermentation bacterium and wood-sugar fermentation bacterium according to 1:1~1:3
(v/v) ratio (such as 1:2), while being inoculated in fermentation medium, and cellulase is added (such as
Jie Neng sections produce Spezyme CP) and/or zytase (such as Jie Neng sections produce Accellerase XC),
Addition is respectively 0.1~15U/g biomass (such as 8U/g biomass, 10U/g biomass, 12
U/g biomass, 15U/g biomass), set 25~38 DEG C of fermentation temperature (such as 30 DEG C, 32 DEG C,
35 DEG C), 36~60 hours (such as 56 hours) of fermenting；
Or,
The seed liquor of glucose fermentation bacterium is first inoculated in fermentation medium, and adds cellulase
(such as Jie Neng sections produce Spezyme CP) and/or zytase (such as Jie Neng sections produce Accellerase XC),
Addition is respectively 0.1~15U/g biomass (such as 8U/g biomass, 10U/g biomass, 12
U/g biomass, 15U/g biomass), at 25~38 DEG C (such as 30 DEG C, 32 DEG C, 35 DEG C)
At a temperature of ferment 24~36 hours (such as 30 hours), inoculate the seed liquor of wood-sugar fermentation bacterium,
Continue 12~50 hours (examples of fermenting at 25~42 DEG C (such as 25 DEG C, 28 DEG C, 38 DEG C or 42 DEG C)
Such as 40 hours or 48 hours), the wherein kind of the seed liquor of glucose fermentation bacterium and wood-sugar fermentation bacterium
Sub- liquid presses 1:1~3:1 ratio is inoculated with.
In a preferred embodiment, the production alcohol fuel described in second aspect present invention
Method, it is characterised in that following i) to vii) in one or more：
I) pre-process before, by biomass straw be crushed to particle diameter for 5~30mm (such as 10mm,
15mm、20mm、30mm)；
Ii) acid solution for 0.1~0.5mol/L (such as 0.2mol/L, 0.3mol/L) sulfuric acid or
Hydrochloric acid solution；
Iii) aqueous slkali is the NaOH solution or ammoniacal liquor of 0.5~1mol/L (such as 0.8mol/L)
Solution；
Iv it is) 12~24h with the time of acid solution or aqueous slkali soaking biomass；
V) after inoculation whiterot fungi, pretreatment time is 10~20 days；
Vi) trace element is potassium dihydrogen phosphate and/or magnesium sulfate；
Preferably, the addition of potassium dihydrogen phosphate be 0.5~3g/L (such as 0.1g/L, 2g/L,
3g/L), the addition of magnesium sulfate be 0.1~1g/L (such as 0.2g/L, 0.5g/L, 0.6g/L,
1g/L)；
Vii) addition of dusty yeast is 0.3~3g/L (such as 0.5g/L, 0.8g/L, 1g/L, 2g/L).
In the present invention, described glucose fermentation bacterium is selected from：Zymomonas mobilis (Zymomonas
Mobilis), saccharomyces cerevisiae (Saccharomyces cerevisiae) and kluyveromyces
(Kluyveromyces marxianus) etc..
In the present invention, described wood-sugar fermentation bacterium is selected from：Neuraspora crassa (Neurospora crassa),
Fusarium oxysporum (Fusarium oxysporum), Pichia pastoris (Pichia pastoris), thermophilic tan pipe capsule
Yeast (Pachysolen tannophilus), shehatae candida (Candida shehatae) is thermophilic
Anaerobic bacteria (Thermoanerobacterethanolicus), bacillus (Bacillus maceran),
Escherichia coli (Escherichia coli), the large intestine bar that can simultaneously utilize glucose and xylose of structure
Bacterium (E.coli), and zymomonas mobilis (Z.mobilis) genetic engineering bacterium.
In a specific embodiment, the method for production alcohol fuel of the present invention, its
Process chart is as shown in Fig. 2 comprise the following steps：
(1) preparation of glucose/xylose mixed liquor
Commercial glucose and xylose sample are weighed respectively, and add water glucose and xylose mixed liquor (the two
Mass ratio is 4:1), wherein, glucose concentration range is：24g/L~180g/L, xylose concentration
Scope is：6g/L~45g/L.
(2) preparation of stalk hydrolysate：
Stalk (maize straw, wheat stalk, rice straw or other biological matter raw material) is crushed
To 5~30mm particle diameters, the diluted acid (sulfuric acid or hydrochloric acid) or 0.5~1mol/L of 0.1~0.5mol/L are added
Aqueous slkali (NaOH or ammoniacal liquor etc.) etc. processes 12~24h, or accesses whiterot fungi (yellow archespore hair
Flat lead fungi (Phanerochaete chrysosporium), worm intend wax bacterium (Ceriporiopsis
Subvermispora) etc.) process 10~20 days, it is through being washed to pH by pretreated stalk
6.0~7.0, drying, the stalk for being pre-processed.The stalk of pretreatment is configured into concentration is
30~90g/L, adds cellulase (such as Jie Neng sections produce Spezyme CP) and/or zytase is (such as
Jie Neng sections produce Accellerase XC), addition is respectively 0.1~10U/g stalks, in its most suitable water
The stalk that pretreatment is hydrolyzed under the conditions of solution temperature and pH prepares the mixed liquor of glucose and xylose.
(3) preparation of glucose fermentation bacterium and glucose fermentation/xylose bacterium seed liquor
Selection glucose fermentation bacterium (such as zymomonas mobilis (Zymomonas mobilis),
Saccharomyces cerevisiae (Saccharomyces cerevisiae) and kluyveromyces (Kluyveromyces
Marxianus at least 1 plant strain in) etc.), is inoculated in its seed culture medium after activation, through training
After supporting 12~36h, the seed liquor of the bacterium is obtained.
Choose the strain (Neuraspora crassa (Neurospora of glucose fermentation/xylose production ethanol
Crassa, Fusarium oxysporum (Fusarium oxysporum), Pichia pastoris (Pichia pastoris),
Pachysolen tannophilus (Pachysolen tannophilus), shehatae candida (Candida
Shehatae), Paecilomyces thermophila (Thermoanaerobacter ethanolicus), gemma bar
Bacterium (Bacillus subtilis) and Escherichia coli (Escherichia coli), and build can be with
Simultaneously using the Escherichia coli of glucose/xylose and zymomonas mobilis genetic engineering bacterium etc.) in
At least 1 plant strain, after activation, according to the strain growth condition, is inoculated in the kind of suitable its growth
Sub- culture medium, after cultivating 12~48h, obtains the seed liquor of the bacterium.
(4) Mixed Microbes substep diastatic fermentation prepares stalk hydrolysate production ethanol
Prepare fermentation medium：Take commercial glucose/xylose mixed liquor or stalk hydrolysate conduct
Carbon source, is configured to concentration for 24g/L~180g/L glucose and concentration are 6g/L~45g/L xyloses
Mixed liquor, adds dusty yeast and other micro- (additions of other trace elements of 0.3~3g/L
Measure and be：0.5~3g/L potassium dihydrogen phosphates, 0.1~1g/L magnesium sulfate), sterilize standby.
At the same time during inoculation fermentation production ethanol, glucose fermentation bacterium and Microorganisms of Fermenting Xylose are taken respectively
Sub- liquid, according to 1:1~1:The ratio of 3 (v/v), while being inoculated in fermentation medium, sets fermentation temperature
25~38 DEG C of degree, after 36~60h of time.Concentration of residual glucose is about at the end for the treatment of process, in zymotic fluid
It is 0.0~1.5g/L, xylose concentration is about 0.0~2.5g/L, and concentration of alcohol is about 12.0~55.0g/L,
Glycerol concentration is about 0.1~2.0g/L.
In step inoculation fermentative production of ethanol, glucose fermentation bacterium seed liquor is first taken, fermentation is set
25~38 DEG C of temperature, after 24~36h of fermentation, by glucose fermentation bacterium seed liquor/Microorganisms of Fermenting Xylose
Liquid 1:1~3:1 (v/v) ratio inoculation the sub- liquid of Microorganisms of Fermenting Xylose, 25~42 DEG C (such as 25 DEG C,
28 DEG C, 38 DEG C or 42 DEG C) continue the 12~36h that ferments.At the end for the treatment of process, Portugal is remained in zymotic fluid
Grape sugar concentration is about 0.0~1.5g/L, and xylose concentration is about 0.0~2.5g/L, and concentration of alcohol is about
12.0~50.0g/L, glycerol concentration is about 0.1~3.0g/L.
(5) Mixed Microbes simultaneous saccharification and fermentation prepares stalk hydrolysate production ethanol
The stalk of pretreatment is configured to concentration for 30~90g/L, add 0.3~3g/L dusty yeast and
Other trace elements, including (0.5~3g/L potassium dihydrogen phosphates, 0.1~1g/L magnesium sulfate), go out
Bacterium obtains fermentation medium, standby.
At the same time during inoculation fermentation production ethanol, glucose fermentation bacterium and Microorganisms of Fermenting Xylose are taken respectively
Sub- liquid, according to 1:1~1:The ratio of 3 (v/v), while being inoculated in fermentation medium, and adds commercially available
Cellulase (such as Jie Neng sections produce Spezyme CP) and/or zytase (such as Jie Neng sections produce
Accellerase XC), addition is respectively 0.1~10U/g stalks；25~38 DEG C of fermentation temperature is set,
After 36~60h of time.At the end for the treatment of process, concentration of residual glucose is about 0.0~1.5g/L in zymotic fluid,
Xylose concentration is about 0.0~2.5g/L, and concentration of alcohol is about 12.0~50.0g/L, and glycerol concentration is about
0.1~2.0g/L.
In step inoculation fermentative production of ethanol, glucose fermentation bacterium seed liquor is first taken, be inoculated in hair
Ferment culture medium, and add commercially available cellulase (such as Jie Neng sections produce Spezyme CP) and/or wood is poly-
Carbohydrase (such as Jie Neng sections produce Accellerase XC), addition is 0.1~10U/g stalks；Hair is set
25~38 DEG C of ferment temperature, after 24~36h of fermentation, by glucose fermentation bacterium seed liquor/Microorganisms of Fermenting Xylose
Sub- liquid 1:1~3:1 (v/v) ratio inoculation the sub- liquid of Microorganisms of Fermenting Xylose, 25~42 DEG C (such as 25 DEG C,
28 DEG C, 38 DEG C or 42 DEG C) continue the 12~48h that ferments.At the end for the treatment of process, Portugal is remained in zymotic fluid
Grape sugar concentration is about 0.0~1.5g/L, and xylose concentration is about 0.0~2.5g/L, and concentration of alcohol is about
12.0~45.0g/L, glycerol concentration is about 0.1~3.0g/L.
The present invention has the advantage that relative to prior art：
Glucose Efficient Conversion can be ethanol by the method for the production alcohol fuel that the present invention is provided,
And xylose farthest changed into ethanol simultaneously, the yield of by-product glycerin is notable in zymotic fluid
Reduce, significantly improve extraction and purification step, the production cost of cellulosic ethanol is substantially reduced.
Brief description of the drawings
Fig. 1 is microbial fermentation xylose synthesizing alcohol and other accessory substances (glycerine and xylitol etc.)
Metabolic map；
Fig. 2 is process chart of the present invention.
Specific embodiment
Technical scheme, but protection scope of the present invention are illustrated with specific embodiment below
Not limited to this.
Embodiment 1
Commercial glucose and xylose sample are weighed respectively, are added water glucose and xylose mixed liquor (Portugal
The mass ratio of grape sugar and xylose is 4:1), wherein, concentration of glucose is：100g/L, xylose is dense
Spend and be：25g/L, the dusty yeast of addition 3g/L and other trace elements be (other trace elements
Addition is：1g/L potassium dihydrogen phosphates, 0.5g/L magnesium sulfate), fermentation medium is obtained, go out
Bacterium is standby.
Choose zymomonas mobilis, it is activated after be inoculated into seed culture medium (seed culture medium matched somebody with somebody
Fang Wei：Glucose 50g/L, KH2PO42g/L, dusty yeast 10g/L, pH7.0), cultivate 12h
Afterwards, the seed liquor of the bacterium is obtained.
Choose pachysolen tannophilus, it is activated after be inoculated into the seed culture medium (formula of seed culture medium
For：Glucose 20g/L, tryptone 3.0g/L, yeast extract 5.0g/L), after culture 48h, obtain
To the seed liquor of the bacterium.
By the seed liquor of zymomonas mobilis seed liquor and pachysolen tannophilus according to 1:1 (v/v's)
Ratio is inoculated in fermentation medium simultaneously, 27 DEG C of fermentation temperature is set, after fermentation 48h, in zymotic fluid
Concentration of residual glucose is about 1.5g/L, and xylose concentration is about 0.5g/L, and concentration of alcohol is about 50.0g/L,
Glycerol concentration is about 0.9g/L.
Embodiment 2
Corn stalk powder is broken to 20mm particle diameters, stalk is configured to concentration for 60g/L, add city
The cellulase sold such as Jie Neng sections produce Spezyme CP and xylanase preparation such as Jie Neng sections and produce
Accellerase XC, addition is respectively 15U/g stalks, water under the conditions of being 7.0,50 DEG C in pH
Solution 48h, prepares the mixed liquor of glucose and xylose, adds the dusty yeast of 2g/L and other are micro-
(other micro- additions are secondary element：3g/L potassium dihydrogen phosphates, 1g/L magnesium sulfate),
Fermentation medium is obtained, is sterilized standby.
Choose saccharomyces cerevisiae, it is activated after be inoculated into seed culture medium (formula of seed culture medium be：
Glucose 40g/L, KH2PO43g/L, dusty yeast 5g/L, pH7.0), after cultivating 18h, obtain
To the seed liquor of the bacterium.
Choose shehatae candida, it is activated after be inoculated into seed culture medium (seed culture medium matched somebody with somebody
Fang Wei：Glucose 30g/L, tryptone 3g/L, yeast extract 5g/L, KH2PO43g/L),
After cultivating 36h, the seed liquor of the bacterium is obtained.
By saccharomyces cerevisiae seed liquor and shehatae candida seed liquor according to 1:The ratio of 2 (v/v) is same
When be inoculated in fermentation medium, 27 DEG C of fermentation temperature is set, after fermentation 48h, Portugal is remained in zymotic fluid
Grape sugar concentration is about 0.5g/L, and xylose concentration is about 0.1g/L, and concentration of alcohol is about 55.0g/L, glycerine
Concentration is about 1.9g/L.
Embodiment 3
Wheat stalk is crushed to 30mm particle diameters, 0.2mol/L H are added2SO4Solution processes 24h,
By pretreated stalk through being washed to pH for 6.0~7.0, drying.The stalk of pretreatment is configured to
Concentration is 70g/L, adds commercially available cellulase such as Jie Neng sections and produces Spezyme CP or zytase
Preparation for example Jie Neng sections produce Accellerase XC, addition be 10U/g stalks, pH6.0,50 DEG C
Hydrolysis 48h, prepares the mixed liquor of glucose and xylose, add 3g/L dusty yeast and other
(other micro- additions are trace element：1g/L potassium dihydrogen phosphates, 0.5g/L sulphur
Sour magnesium), fermentation medium is obtained, sterilize standby.
Choose kluyveromyces, it is activated after be inoculated into seed culture medium (formula of seed culture medium be：
Glucose 50g/L, KH2PO45g/L, dusty yeast 10g/L, pH 7.0), after cultivating 24h,
Obtain the seed liquor of the bacterium.
Choose Fusarium oxysporum, it is activated after be inoculated with its seed culture medium (formula of seed culture medium be：
Glucose 20g/L, tryptone 3g/L, yeast extract 3g/L), after cultivating 30h, it is somebody's turn to do
The seed liquor of bacterium.
Kluyveromyces seed liquor is first taken, fermentation medium is inoculated in, after the 30h that fermented at 35 DEG C,
By 1:1 (v/v) is inoculated with Fusarium oxysporum seed liquor, and adjustment fermentation temperature is 28 DEG C, continues the 36h that ferments.
At the end for the treatment of process, concentration of residual glucose is about 0.1g/L in zymotic fluid, and xylose concentration is 2.0g/L,
Concentration of alcohol is 40.0g/L, and glycerol concentration is 1.7g/L.
Embodiment 4
Corn stalk powder is broken to 20mm particle diameters, worm is accessed and is intended wax bacterium treatment 20 days, will pre-processed
Stalk afterwards is 6.0~7.0 through being washed to pH, drying.The stalk of pretreatment is configured into concentration is
60g/L, (other micro- additions are to add the dusty yeast and other trace elements of 2g/L：
1g/L potassium dihydrogen phosphates, 0.6g/L magnesium sulfate), fermentation medium is obtained, sterilize standby.
Choose zymomonas mobilis, it is activated after be inoculated into seed culture medium (seed culture medium matched somebody with somebody
Fang Wei：Glucose 50g/L, KH2PO42g/L, dusty yeast 10g/L, pH7.0), through cultivating 12h
Afterwards, the seed liquor of the bacterium is obtained.
Choose Pichia pastoris, it is activated after be inoculated into seed culture medium (formula of seed culture medium be：
Glucose 30g/L, tryptone 3g/L, yeast extract 5g/L, KH2PO43g/L), through culture
After 24h, the seed liquor of the bacterium is obtained.
Zymomonas mobilis seed liquor and Pichia pastoris seed liquor are taken respectively, according to 1:1 (v/v's)
Ratio is inoculated in fermentation medium simultaneously, and adds commercially available cellulase such as Jie Neng sections product
Spezyme CP and xylanase preparation such as Jie Neng sections produce Accellerase XC, and addition is respectively
12U/g stalks, set 38 DEG C of fermentation temperature, after fermentation 56h, concentration of residual glucose in zymotic fluid
About 0.0g/L, xylose concentration is about 0.3g/L, and concentration of alcohol is about 50.0g/L, and glycerol concentration is about
0.9g/L。
Embodiment 5
Rice straw is crushed to 15mm particle diameters, 0.5mol/LNaOH solution treatment 24h is added,
By pretreated stalk through being washed to pH for 6.0~7.0, drying.The stalk of pretreatment is configured to
Concentration is 30g/L, and (other trace elements add to add the dusty yeast and other trace elements of 1g/L
Dosage is：2g/L potassium dihydrogen phosphates, 1g/L magnesium sulfate), fermentation medium is obtained, sterilize
It is standby.
Choose saccharomyces cerevisiae, it is activated after be inoculated into seed culture medium (formula of seed culture medium be：
Glucose 40g/L, KH2PO43g/L, dusty yeast 5g/L, pH7.0), after cultivating 18h, obtain
To the seed liquor of the bacterium.
Choose thermophilc anaerobe, it is activated after be inoculated with its seed culture medium (formula of seed culture medium
For：Glucose 20g/L, NH4C 10.90g/L, NaCl 0.90g/L, MgCl20.40g/L,
KH2PO40.75g/L, K2HPO41.50g/L, Trypticase 2.00g/L, Yeast extract
1.00g/L, FeCl2 2.50×10-3G/L, Cysteine-HCl 0.75g/L), after cultivating 24h,
Obtain the seed liquor of the bacterium.
Saccharomyces cerevisiae seed liquor is first taken, fermentation medium is inoculated in, and add commercially available cellulase
Accellerase XC are produced as Jie Neng sections produce Spezyme CP and xylanase preparation such as Jie Neng sections, is added
Dosage is respectively 8U/g stalks, 38 DEG C of fermentation temperature is set, after fermentation 36h, by 1:1(v/v)
Inoculation thermophilc anaerobe seed liquor, adjustment fermentation temperature is 42 DEG C, continues the 48h that ferments.Treat process
At the end of, concentration of residual glucose is about 0.5g/L in zymotic fluid, and xylose concentration is 1.0g/L, second
Determining alcohol is 27.0g/L, and glycerol concentration is 0.7g/L.
Claims (10)
1. a kind of method for producing alcohol fuel, comprises the following steps：
1) with mixed liquor or biomass (such as maize straw, Wheat Straw of glucose and xylose
Stalk or rice straw) used as carbon source, addition dusty yeast and trace element are fermented hydrolysate
Culture medium；
2) by the seed liquor of the seed liquor of glucose fermentation bacterium and wood-sugar fermentation bacterium according to 1:1~1:3
(v/v) ratio, while fermentation medium is inoculated in, the fermentation 36~60 at a temperature of 25~38 DEG C
Hour；
Or,
The seed liquor of glucose fermentation bacterium is first inoculated in fermentation medium, in 25~38 DEG C of temperature
Lower fermentation 24~36 hours, inoculates the seed liquor of wood-sugar fermentation bacterium, continues to ferment at 25~42 DEG C
12~36 hours, the seed liquor of the wherein seed liquor of glucose fermentation bacterium and wood-sugar fermentation bacterium was pressed
1:1~3:1 ratio is inoculated with.
2. described in claim 1 production alcohol fuel method, it is characterised in that glucose and
Concentration of glucose is 24g/L~180g/L in the mixed liquor of xylose, and xylose concentration is 6g/L~45g/L.
3. described in claim 1 production alcohol fuel method, it is characterised in that described life
The preparation method of Substance P hydrolysis product is comprised the following steps：
With acid solution or aqueous slkali soaking biomass (such as maize straw, wheat stalk or paddy rice straw
Stalk) pre-processed, or by biomass (such as maize straw, wheat stalk or rice straw)
Access whiterot fungi (such as Phanerochaete chrysosporium (such as Phanerochaete chrysosporium),
Worm intends wax bacterium (Ceriporiopsis subvermispora) etc.) pre-processed, after pretreatment
Biomass material to adjust to pH be 6.0~7.0, drying adds water and is configured to concentration for 30~90g/L
Solution, addition is respectively 0.1~15U/g biomass, regulation hydrolysis temperature and enzymolysis pH value,
Be hydrolyzed reaction, obtains biomass hydrolysate；
Or, the preparation method of the biomass hydrolysate is comprised the following steps：
Matter is added water the solution for being configured to that concentration is 30~90g/L, and addition cellulase is (such as outstanding energy
Section produces Spezyme CP) and/or zytase (such as Jie Neng sections produce Accellerase XC), addition
Amount is respectively 0.1~15U/g biomass, regulation hydrolysis temperature and enzymolysis pH value, is hydrolyzed anti-
Should, biomass hydrolysate is obtained,
Or, the preparation method of described biomass hydrolysate is comprised the following steps：
With acid solution or aqueous slkali soaking biomass (such as maize straw, wheat stalk or paddy rice straw
Stalk) pre-processed, or by biomass (such as maize straw, wheat stalk or rice straw)
Access whiterot fungi (such as Phanerochaete chrysosporium (such as Phanerochaete chrysosporium),
Worm intends wax bacterium (Ceriporiopsis subvermispora) etc.) pre-processed, after pretreatment
Biomass material to adjust to pH be 6.0~7.0, drying adds water and is configured to concentration for 30~90g/L
Solution, addition cellulase (such as Jie Neng sections produce Spezyme CP) and/or zytase is (such as
Jie Neng sections produce Accellerase XC), addition is respectively 0.1~15U/g biomass, adjusts enzyme
Solution temperature and enzymolysis pH value, be hydrolyzed reaction, obtains biomass hydrolysate.
4. described in any one of claim 1-3 production alcohol fuel method, it is characterised in that
Described trace element be potassium dihydrogen phosphate and/or magnesium sulfate,
Preferably, the addition of potassium dihydrogen phosphate is 0.5~3g/L, and the addition of magnesium sulfate is 0.1~1
g/L。
5. described in any one of claim 1-3 production alcohol fuel method, it is characterised in that
The addition of dusty yeast is 0.3~3g/L.
6. described in claim 3 production alcohol fuel method, it is characterised in that it is following i)
To viii) in one or more：
I) before pre-processing, biomass straw is crushed to particle diameter for 5~30mm；
Ii) acid solution is the sulfuric acid or hydrochloric acid solution of 0.1~0.5mol/L；
Iii) aqueous slkali is the NaOH solution or ammonia spirit of 0.5~1mol/L；
Iv it is) 12~24h with the time of acid solution or aqueous slkali soaking biomass；
V) after inoculation whiterot fungi, pretreatment time is 10~20 days；
Vi) hydrolysis temperature is 45~55 DEG C；
Vii) enzymolysis pH value is 3.0~11.0；
Viii) enzymolysis time is 40~55 hours.
7. a kind of method for producing alcohol fuel, comprises the following steps：
1) acid solution or aqueous slkali soaking biomass (such as maize straw, wheat stalk or water are used
Rice straw) pre-processed, or by biomass material (such as maize straw, wheat stalk or
Rice straw) access whiterot fungi (such as Phanerochaete chrysosporium (such as Phanerochaete
Chrysosporium), worm intends wax bacterium (Ceriporiopsis subvermispora etc.) carries out pre- place
Reason, it is 6.0~7.0 that pretreated biomass material is adjusted to pH, and drying adds water and is configured to
Concentration is the solution of 30~90g/L, adds dusty yeast and trace element, obtains fermentation medium；
2) by the seed liquor of the seed liquor of glucose fermentation bacterium and wood-sugar fermentation bacterium according to 1:1~1:3
(v/v) ratio, while fermentation medium is inoculated in, and (such as Jie Neng sections produce to add cellulase
Spezyme CP) and/or zytase (such as Jie Neng sections produce Accellerase XC), addition point
Wei not 0.1~15U/g biomass, 25~38 DEG C of fermentation temperature of setting, 36~60h of fermentation；
Or,
The seed liquor of glucose fermentation bacterium is first inoculated in fermentation medium, and adds cellulase
(such as Jie Neng sections produce Spezyme CP) and/or zytase (such as Jie Neng sections produce Accellerase XC),
Addition is respectively 0.1~15U/g biomass, is fermented 24~36 hours at a temperature of 25~38 DEG C,
The seed liquor of wood-sugar fermentation bacterium is inoculated, continues to ferment 12~50 hours at 25~42 DEG C, wherein Portugal
The seed liquor of the zymogenic seed liquor of grape sugar and wood-sugar fermentation bacterium presses 1:1~3:1 ratio is inoculated with.
8. the method for the production alcohol fuel described in claim 7, it is characterised in that it is following i) extremely
Vii one or more in)：
I) before pre-processing, biomass straw is crushed to particle diameter for 5~30mm；
Ii) acid solution is the sulfuric acid or hydrochloric acid solution of 0.1~0.5mol/L；
Iii) aqueous slkali is the NaOH solution or ammonia spirit of 0.5~1mol/L；
Iv it is) 12~24h with the time of acid solution or aqueous slkali soaking biomass；
V) after inoculation whiterot fungi, pretreatment time is 10~20 days；
Vi) trace element is potassium dihydrogen phosphate and/or magnesium sulfate；
Preferably, the addition of potassium dihydrogen phosphate is 0.5~3g/L, and the addition of magnesium sulfate is 0.1~1
g/L；
Vii) addition of dusty yeast is 0.3~3g/L.
9. described in any one of claim 1 to 8 production alcohol fuel method, it is characterised in that
Described glucose fermentation bacterium is selected from：Zymomonas mobilis (Zymomonas mobilis), wine brewing
Yeast (Saccharomyces cerevisiae) and kluyveromyces (Kluyveromyces marxianus)
Deng.
10. described in any one of claim 1 to 8 production alcohol fuel method, it is characterised in that
Described wood-sugar fermentation bacterium is selected from：Neuraspora crassa (Neurospora crassa), Fusarium oxysporum
(Fusarium oxysporum), Pichia pastoris (Pichia pastoris), pachysolen tannophilus
(Pachysolen tannophilus), shehatae candida (Candida shehatae), thermophilic anaerobic
Bacterium (Thermoanerobacterethanolicus), bacillus (Bacillus maceran), large intestine
Bacillus (Escherichia coli), the Escherichia coli (E. that can simultaneously utilize glucose and xylose of structure
), and zymomonas mobilis (Z.mobilis) genetic engineering bacterium coli.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107142297A (en) * | 2017-07-18 | 2017-09-08 | 天津科技大学 | A kind of synchronous saccharification common fermentation method |
| CN108642118A (en) * | 2018-07-06 | 2018-10-12 | 佛山皖阳生物科技有限公司 | A kind of biomass mixture of producing and ethanol and the method for producing and ethanol |
| CN109402180A (en) * | 2018-12-04 | 2019-03-01 | 浙江华康药业股份有限公司 | A method of alcohol fuel is prepared using containing sugared material solution |
| CN111500338A (en) * | 2020-05-28 | 2020-08-07 | 肖虎来 | Biomass fuel with high burning speed and manufacturing method thereof |
| CN115181681A (en) * | 2022-09-06 | 2022-10-14 | 山东蓬勃生物科技有限公司 | Microbial agent for preparing bioethanol, preparation method and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1880416A (en) * | 2005-06-14 | 2006-12-20 | 河南农业大学 | Process for preparing fuel ethanol by using straw fiber materials |
| US20080138872A1 (en) * | 2005-03-17 | 2008-06-12 | Novozymes North America, Inc. | Processes for Producing Fermentation Products |
| CN101781634A (en) * | 2009-12-04 | 2010-07-21 | 天津大学 | Recombinant zymomonas mobilis capable of producing ethanol by using xylose and fermentation method thereof |
| CN101914578A (en) * | 2010-07-21 | 2010-12-15 | 浙江大学宁波理工学院 | Method for producing ethanol by using citrus peel residue as raw material |
| CN103805673A (en) * | 2014-03-01 | 2014-05-21 | 青岛科技大学 | Method of producing stalk ethanol by means of mixed fermentation of transgenetic yeast |
| CN104805133A (en) * | 2014-01-27 | 2015-07-29 | 中粮营养健康研究院有限公司 | Method for producing ethanol through co-fermenting C5 and C6 by using microbes |
-
2015
- 2015-12-31 CN CN201511021364.9A patent/CN106929543A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080138872A1 (en) * | 2005-03-17 | 2008-06-12 | Novozymes North America, Inc. | Processes for Producing Fermentation Products |
| CN1880416A (en) * | 2005-06-14 | 2006-12-20 | 河南农业大学 | Process for preparing fuel ethanol by using straw fiber materials |
| CN101781634A (en) * | 2009-12-04 | 2010-07-21 | 天津大学 | Recombinant zymomonas mobilis capable of producing ethanol by using xylose and fermentation method thereof |
| CN101914578A (en) * | 2010-07-21 | 2010-12-15 | 浙江大学宁波理工学院 | Method for producing ethanol by using citrus peel residue as raw material |
| CN104805133A (en) * | 2014-01-27 | 2015-07-29 | 中粮营养健康研究院有限公司 | Method for producing ethanol through co-fermenting C5 and C6 by using microbes |
| CN103805673A (en) * | 2014-03-01 | 2014-05-21 | 青岛科技大学 | Method of producing stalk ethanol by means of mixed fermentation of transgenetic yeast |
Non-Patent Citations (7)
| Title |
|---|
| DEVENDRA PRASAD MAURYA ET AL.: "An overview of key pretreatment processes for biological conversion of lignocellulosic biomass to bioethanol", 《3 BIOTECH》 * |
| RASMUS LUND ANDERSEN ET AL.: "Continuous Ethanol Fermentation of Pretreated Lignocellulosic Biomasses，Waste Biomasses，Molasses and Syrup Using the Anaerobic，Thermophilic Bacterium Thermoanaerobacter italicus Pentocrobe 411", 《PLOS ONE》 * |
| 刘红梅: "多菌种混合共发酵甘蔗渣纤维素和半纤维素制备燃料乙醇的研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 孙传伯: "《生物质能源工程》", 30 September 2015, 合肥工业大学出版社 * |
| 张继泉等: "利用木质纤维素生产燃料酒精的研究进展", 《酿酒科技》 * |
| 李玉英: "《发酵工程》", 31 May 2009, 中国农业大学出版社 * |
| 温辉梁: "《生物化工产品生产技术》", 31 December 2004, 江西科学技术出版社 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107142297A (en) * | 2017-07-18 | 2017-09-08 | 天津科技大学 | A kind of synchronous saccharification common fermentation method |
| CN108642118A (en) * | 2018-07-06 | 2018-10-12 | 佛山皖阳生物科技有限公司 | A kind of biomass mixture of producing and ethanol and the method for producing and ethanol |
| CN109402180A (en) * | 2018-12-04 | 2019-03-01 | 浙江华康药业股份有限公司 | A method of alcohol fuel is prepared using containing sugared material solution |
| CN111500338A (en) * | 2020-05-28 | 2020-08-07 | 肖虎来 | Biomass fuel with high burning speed and manufacturing method thereof |
| CN111500338B (en) * | 2020-05-28 | 2021-09-07 | 广东洁冠科技有限公司 | Biomass fuel manufacturing equipment with high burning speed |
| CN115181681A (en) * | 2022-09-06 | 2022-10-14 | 山东蓬勃生物科技有限公司 | Microbial agent for preparing bioethanol, preparation method and application thereof |
| CN115181681B (en) * | 2022-09-06 | 2023-01-31 | 山东蓬勃生物科技有限公司 | Microbial agent for preparing bioethanol, preparation method and application thereof |
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