CN103060217A - Recombinant yeast strain capable of efficiently metabolizing xylose and application thereof - Google Patents
Recombinant yeast strain capable of efficiently metabolizing xylose and application thereof Download PDFInfo
- Publication number
- CN103060217A CN103060217A CN201210507117XA CN201210507117A CN103060217A CN 103060217 A CN103060217 A CN 103060217A CN 201210507117X A CN201210507117X A CN 201210507117XA CN 201210507117 A CN201210507117 A CN 201210507117A CN 103060217 A CN103060217 A CN 103060217A
- Authority
- CN
- China
- Prior art keywords
- xylose
- ethanol
- recombinant yeast
- yeast strain
- l2612pr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention discloses a recombinant yeast strain (Saccharomyces cerevisiae)) capable of efficiently metabolizing xylose, which is named SyBE005. The recombinant yeast strain has been collected in Common Microorganism Center of China Committee for Culture Collection of Microorganisms, the collection number is CGMCC No.6634, and the recombinant yeast strain has the capacity of efficiently metabolizing xylose to produce ethanol. The recombinant yeast strain SyBE005 can effectively ferment xylose to generate ethanol. The ethanol yield reaches 64% of the theoretical yield, and the yield of the byproduct xylitol is lower than 12%; and thus, the recombinant yeast strain can efficiently convert xylose into ethanol, and is an excellent strain utilizing cellulose hydrolysate.
Description
Technical field
The invention belongs to technical field of bioengineering, be specifically related to the restructuring yeast strains that a strain can xylose fermentation ethanol.
Background technology
Utilize lignocellulosic materials for fuel ethanol, not only can reduce the production cost of alcohol fuel, and all play a very important role at aspects such as improving environment and refuse processing.China is as a large agricultural country, and the agricultural waste material resources such as stalk are quite abundant, and this provides advantageous condition for the Fuel Alcohol Development industry.General bacterial strain as industrial production ethanol is yeast saccharomyces cerevisiae, although natural yeast saccharomyces cerevisiae can be good at utilizing the hexose in the lignocellulose hydrolyzate to produce ethanol, but lacks the pentose to rich content, for example the metabolic capacity of wood sugar.Therefore make up the engineering strain that can efficiently utilize wood sugar the production cost that reduces alcohol fuel is had crucial meaning.
At present China is making some progress aspect the saccharomyces cerevisiae engineered yeast strain that utilizes the metabolic engineering method to make up can to utilize wood sugar to produce ethanol, but still can not satisfy the requirement of production, be mainly manifested in xylose utilization slowly, the accumulation of by product Xylitol too much and the aspect such as alcohol getting rate is low.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, the restructuring yeast strains of plant height effect fermenting xylose is provided.
Second purpose of the present invention provides the purposes of the restructuring yeast strains of above-mentioned plant height effect fermenting xylose.
Technical scheme of the present invention is summarized as follows:
The restructuring yeast strains of one plant height effect fermenting xylose, Classification And Nomenclature: yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) called after SyBE005, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, deposit number is CGMCC No.6634, and it has the ability of efficient fermenting xylose producing and ethanol.
The purposes of above-mentioned bacterial strains fermenting xylose producing and ethanol.
Advantage of the present invention:
Restructuring yeast strains SyBE005 of the present invention can effectively utilize wood-sugar fermentation to produce ethanol.Alcohol yied reaches 64% of theoretical yield, and the productive rate of by product Xylitol is below 12%, and can transform efficiently wood sugar is ethanol, is the good bacterial strain that utilizes cellulosic hydrolysate.
The restructuring yeast strains of efficient fermenting xylose of the present invention (Saccharomyces cerevisiae) called after SyBE005, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on September 27th, 2012, be called for short CGMCC, deposit number is CGMCC No.6634.The address is in the Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
Description of drawings
Fig. 1 is the plasmid map that contains Xylose reductase gene encoder block, xylose dehydrogenase gene encoder block and xylulokinase gene encoder block.
Fig. 2 is the plasmid pRS305-XDH collection of illustrative plates that contains the xylose dehydrogenase gene encoder block.
Fig. 3 is the plasmid pRS-RKI1-TKL1 collection of illustrative plates that contains ribose phosphoric acid isomerase genes encoding frame, tkt gene encoder block.
Fig. 4 is the plasmid pAUR-RPE1-TAL1 collection of illustrative plates that contains pentose phosphate epimerase genes encoding frame, transaldolase genes encoding frame.
Fig. 5 is bacterial strain L2612PR, L2612PR-DPPP, SyBE005 xylose utilization situation when fermenting in the fermention medium that contains the 20g/L wood sugar.
Fig. 6 is bacterial strain L2612PR, L2612PR-DPPP, SyBE005 ethanol production when fermenting in the fermention medium that contains the 20g/L wood sugar.
Fig. 7 is bacterial strain L2612PR, L2612PR-DPPP, SyBE005 Xylitol production when fermenting in the fermention medium that contains the 20g/L wood sugar.
Embodiment
The present invention is further illustrated below in conjunction with specific embodiment, and embodiments of the invention are in order to enable those skilled in the art to understand better the present invention, but the present invention are not done any restriction.
The structure of restructuring yeast strains SyBE005 and screening
Starting strain: starting strain L2612 is that Thomas professor Jeffries of University of Wisconsin at Madison grants, and genotype is MAT alpha, leu2, ura3, trp1.Leucine, uridylic and tryptophane defective.
Substratum
Screening culture medium A: synthetic nitrogenous source YNB 6.7g/L, kilnitamin powder 2g/L(specifically fills a prescription with reference to yeast genetics method test guides such as [U.S.] D.C. Ambergs), leucine 100mg/L, tryptophane 100mg/L, glucose 20g/L.Screening culture medium B: synthetic nitrogenous source YNB 6.7g/L, kilnitamin powder 2g/L, tryptophane 100mg/L, glucose 20g/L.Screening culture medium C: synthetic nitrogenous source YNB 6.7g/L, kilnitamin powder 2g/L, glucose 20g/L.Screening culture medium D: synthetic nitrogenous source YNB 6.7g/L, kilnitamin powder 2g/L, short stalk mycin A 0.5mg/L, glucose 20g/L.Domestication substratum: synthetic nitrogenous source YNB 6.7g/L, kilnitamin powder 2g/L, wood sugar 50g/L.In the solid medium, add the agar of 20g/L.
The structure of recombinant bacterial strain L2612PR-DPPP sets out
Take bacterial strain L2612 genome as template, clone promotor TDH1p, TDH3p, PGK1p, xylulokinase gene XKS1(contains terminate self), pentose phosphate epimerase gene RPE1, ribose phosphoric acid isomerase gene RKI1, tkt gene TKL1, transaldolase gene TAL1, terminator sequence PGK1t.
By merging round pcr synthetic Xylose reductase gene XR, xylose dehydrogenase gene XDH are connected with promotor PGK1, terminator PGK1t respectively, promotor PGK1 is connected with xylulokinase gene XKS1, then three gene expression frames are connected to carrier YIplac211(in turn and are purchased from ATCC) on, form expression plasmid YIplac211-XRXDHXK(Fig. 1).
Xylose reductase gene XR sequence is with shown in the SEQ ID NO:1.
Xylose dehydrogenase gene XDH sequence is with shown in the SEQ ID NO:2.
The xylose dehydrogenase gene expression cassette of above structure is cut out from the upper enzyme of plasmid YIplac211-XRXDHXK, again is cloned into carrier pRS305(and is purchased from ATCC) on, plasmid pRS305-XDH (Fig. 2) formed.
Utilize and merge round pcr structure pentose phosphate epimerase expression casette TDH1p-RPE1-PGK1t, transaldolase expression casette PGK1p-TAL1-PGK1t, ribose phosphoric acid isomerase expression casette PGK1p-RKI1-PGK1t, tkt gene expression cassette TDH3p-TKL1-PGK1t.Expression casette PGK1p-RKI1-PGK1t and TDH3p-TKL1-PGK1t are connected to carrier pRS304(are purchased from ATCC) on, carrier pRS-RKI1-TKL1 (Fig. 3) formed.Expression casette TDH1p-RPE1-PGK1t and expression casette PGK1p-TAL1-PGK1t are connected to the purchase of the precious biotech firm in carrier pAUR101(Dalian successively), carrier construction pAUR-RPE1-TAL1 (Fig. 4).
With restriction enzyme A pa I linearizing YIplac211-XRXDHXK, transform bacterial strain L2612 by the Lithium Acetate method, obtain recombinant bacterial strain L2612PR in screening culture medium A screening.And on the basis of this recombinant bacterial strain, transforming plasmid pRS305-XDH with the Lithium Acetate method, B obtains recombinant bacterial strain in screening culture medium.With restriction enzyme EcoRI linearization plasmid pRS-RKI1-TKL1, transform recombinant bacterial strain obtained in the previous step with the Lithium Acetate method, express obtaining recombinant bacterial strain at screening culture medium C.And on the basis of this bacterial strain, further transform with StuI linearizing plasmid pAUR-RPE1-TAL1, at the screening culture medium D recombinant bacterial strain L2612PR-DPPP that obtains setting out.
The structure of mutant strain SyBE005 and screening
With bacterial strain L2612PR-DPPP with initial OD
600=0.2 receives among the 250mL that contains 50mL domestication substratum, and 30 ℃, 200 rev/mins aerobics were cultivated 3 days.Measure the OD of culture
600, and with initial OD
600=0.2 is transferred in the fresh domestication substratum, repeats to cultivate the OD of bacterium liquid after 10 times
600Remain unchanged, continue to repeat to cultivate 10 times.The bacterium liquid that takes a morsel, the solid plate coating at the domestication substratum after the dilution is cultivated, and obtains single bacterium colony bacterial strain.From wherein choosing 20 bacterium colonies of bacterium colony size maximum, be inoculated into respectively in the 15mL test tube that contains 2mL liquid screening culture medium C, under 30 ℃, 200 rev/mins condition, cultivated 2 days.Get 100 μ L bacterium liquid and be transferred among the fresh fermention medium A of 3mL, anaerobic is cultivated under 30 ℃, 200 rev/mins condition.Use the rubber stopper seal test tube mouth with exhaust needle.Anaerobic was cultivated after 24 hours, measured the OD of bacterium liquid
600, remaining Xylose Content and meta-bolites ethanol, glycerine, Determination of Xylitol.From the single bacterium colony of 20 strains, obtained the minimum bacterial strain SyBE005 of the remaining wood sugar of a strain.
Analytical procedure:
With the thalline light absorption value (OD of 722 type spectrophotometers in 600nm place mensuration
600) the sign cell concentration.The concentration of wood sugar, ethanol, Xylitol, glycerine is measured with high performance liquid chromatography (Waters1515).Chromatographic column is AminexHPX-87H, column temperature: 65 ℃; Detector: Waters differential detector 2421, detector temperature are 40 ℃.Moving phase is the 5mM sulphuric acid soln, and flow velocity 0.6mL/min, sample size are 10 μ L.
The wood-sugar fermentation of embodiment 2 bacterial strain L2612PR, L2612PR-DPPP and SyBE005 relatively
1. test materials: bacterial strain L2612PR, L2612PR-DPPP, SyBE003
2. test method:
Seed culture medium 1: synthetic nitrogenous source YNB 6.7g/L, kilnitamin powder 2g/L, glucose 20g/L, leucine 100mg/L, tryptophane 100mg/L.
Seed culture medium 2: synthetic nitrogenous source YNB 6.7g/L, kilnitamin powder 2g/L, glucose 20g/L.
Fermention medium: yeast soaks powder 10g/L, peptone 20g/L, wood sugar 20g/L.
Connect a ring L2612PR bacterium colony to 50mL seed culture medium 1 from fresh inclined-plane.Similarly, meet bacterium colony L2612PR-DPPP and SyBE005 from fresh inclined-plane to 50mL seed culture medium 2.Under 30 ℃, 200 rev/mins condition, cultivated 24 hours.With initial cell concentration OD
600=1.0 are inoculated in the 100mL fermention medium, cultivate under 30 ℃, 150 rev/mins conditions, ferment 60 hours.Detect the concentration of cell concentration, xylose concentration and product ethanol, Xylitol, glycerine.
3. analytical procedure:
With embodiment 1.
4. test-results:
Shown in Figure 5, bacterial strain SyBE005 has utilized wood sugar fully in 48 hours, and bacterial strain L2612PR, L2612PR-DPPP have only utilized approximately 60% wood sugar, and the maximum wood sugar specific consumption rate of SyBE005 reaches 0.301g/g dry weight/h; After the fermentation ends, the ethanol production of L2612PR, L2612PR-DPPP, SyBE005 has reached respectively 2.02g/L, 2.97g/L, 6.85g/L.The alcohol getting rate that L2612PR, L2612PR-DPPP are corresponding is respectively 0.15g/g wood sugar, 0.20g/g wood sugar, the fermenting alcohol yield of SyBE005 reaches the 0.33g/g wood sugar, improved respectively 120% and 65% with respect to L2612PR, L2612PR-DPPP, as shown in Figure 6; After the fermentation ends, the Determination of Xylitol of L2612PR, L2612PR-DPPP, SyBE005 has reached respectively 6.24g/L, 6.29g/L, 2.43g/L.The xylitol yield of L2612PR, L2612PR-DPPP reaches 0.48g/g wood sugar, 0.42g/g wood sugar, and the xylitol yield of SyBE005 is reduced to the 0.12g/g wood sugar, has reduced by 3,2.5 times with respect to L2612PR, L2612PR-DPPP, as shown in Figure 7.
Claims (2)
1. a plant height is imitated restructuring yeast strains (Saccharomyces cerevisiae) the called after SyBE005 of fermenting xylose, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, deposit number is CGMCC No.6634, and it has the ability of efficient fermenting xylose producing and ethanol.
2. the purposes of the described bacterial strain fermenting xylose of claim 1 producing and ethanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210507117.XA CN103060217B (en) | 2012-11-29 | 2012-11-29 | Recombinant yeast strain capable of efficiently metabolizing xylose and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210507117.XA CN103060217B (en) | 2012-11-29 | 2012-11-29 | Recombinant yeast strain capable of efficiently metabolizing xylose and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103060217A true CN103060217A (en) | 2013-04-24 |
| CN103060217B CN103060217B (en) | 2014-12-17 |
Family
ID=48103117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210507117.XA Active CN103060217B (en) | 2012-11-29 | 2012-11-29 | Recombinant yeast strain capable of efficiently metabolizing xylose and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103060217B (en) |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104073534A (en) * | 2014-07-04 | 2014-10-01 | 天津大学 | Method for reducing dosage of cellulose hydrolase in simultaneous saccharification and cofermentation |
| CN104073525A (en) * | 2014-07-04 | 2014-10-01 | 天津大学 | Method for producing ethanol by virtue of simultaneous saccharification and co-fermentation |
| CN104419701A (en) * | 2013-08-29 | 2015-03-18 | 天津大学 | Rapid assembling method of multi-fragment DNA yeast |
| CN104561132A (en) * | 2015-02-15 | 2015-04-29 | 天津大学 | Method for producing ethanol from mixed bacteria with inhibitor |
| CN104611379A (en) * | 2015-03-04 | 2015-05-13 | 天津大学 | Microbial ethanol fermentation method |
| CN104789605A (en) * | 2015-02-10 | 2015-07-22 | 天津大学 | Method for fermentation production of ethanol in presence of inhibitor |
| CN105368731A (en) * | 2015-11-10 | 2016-03-02 | 四川大学 | Saccharomyces cerevisiae strain for expressing xylose isomerase and construction method |
| CN105368730A (en) * | 2015-11-10 | 2016-03-02 | 四川大学 | Saccharomyces cerevisiae strain for producing ethanol by quick fermentation of xylose and construction method |
| CN105483033A (en) * | 2015-12-25 | 2016-04-13 | 天津大学 | Strain as well as preparation method, application and fermentation method thereof |
| CN105567744A (en) * | 2016-01-21 | 2016-05-11 | 天津大学 | Method for improving utilization rate of xylose in lignocellulose hydrolysate |
| CN105567745A (en) * | 2016-01-21 | 2016-05-11 | 天津大学 | Method for improving utilization of xylose in lignocellulose hydrolysate |
| CN104419716B (en) * | 2013-08-27 | 2017-12-29 | 天津大学 | A kind of standardization, high-precision, general functional module construction method |
| CN107916235A (en) * | 2017-11-15 | 2018-04-17 | 天津大学 | A kind of method that restructuring yeast strains and microorganism mix bacterium electricity production |
| CN108823113A (en) * | 2018-06-15 | 2018-11-16 | 首都师范大学 | The industrial strain and method of efficient xylose metabolism producing and ethanol |
| US10759727B2 (en) | 2016-02-19 | 2020-09-01 | Intercontinental Great Brands Llc | Processes to create multiple value streams from biomass sources |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1141057A (en) * | 1993-11-08 | 1997-01-22 | 普渡研究基金会 | Recombinant yeasts for effective fermentation of glucose and xylose |
| CN1225125A (en) * | 1996-05-06 | 1999-08-04 | 普渡研究基金会 | Stable recombinant yeasts for fermenting xylose to ethanol |
| CN101792753A (en) * | 2009-12-16 | 2010-08-04 | 安徽丰原发酵技术工程研究有限公司 | Construction method for production of recombinant yeast of ethanol by fermented xylose |
| CN102127512A (en) * | 2010-12-20 | 2011-07-20 | 浙江大学 | Saccharomyces cerevisiae engineering bacterium capable of fermenting xylose |
-
2012
- 2012-11-29 CN CN201210507117.XA patent/CN103060217B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1141057A (en) * | 1993-11-08 | 1997-01-22 | 普渡研究基金会 | Recombinant yeasts for effective fermentation of glucose and xylose |
| CN1225125A (en) * | 1996-05-06 | 1999-08-04 | 普渡研究基金会 | Stable recombinant yeasts for fermenting xylose to ethanol |
| CN101792753A (en) * | 2009-12-16 | 2010-08-04 | 安徽丰原发酵技术工程研究有限公司 | Construction method for production of recombinant yeast of ethanol by fermented xylose |
| CN102127512A (en) * | 2010-12-20 | 2011-07-20 | 浙江大学 | Saccharomyces cerevisiae engineering bacterium capable of fermenting xylose |
Non-Patent Citations (2)
| Title |
|---|
| BYRON C.H.CHU等: "Genetic improvement of Saccharomyces cerevisiae for xylose fermentation", 《BIOTECHNOLOGY ADVANCES》, vol. 25, 24 April 2007 (2007-04-24), pages 425 - 441 * |
| KAISA KARHUMAA等: "Investigation of limiting metabolic steps in the utilization of xylose by recombinant Saccharomyces cerevisiae using metabolic engineering", 《YEAST》, vol. 22, 31 December 2005 (2005-12-31) * |
Cited By (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104419716B (en) * | 2013-08-27 | 2017-12-29 | 天津大学 | A kind of standardization, high-precision, general functional module construction method |
| CN104419701A (en) * | 2013-08-29 | 2015-03-18 | 天津大学 | Rapid assembling method of multi-fragment DNA yeast |
| CN104419701B (en) * | 2013-08-29 | 2019-01-11 | 天津大学 | The quick assemble method of the yeast of multiple clips DNA |
| CN104073525A (en) * | 2014-07-04 | 2014-10-01 | 天津大学 | Method for producing ethanol by virtue of simultaneous saccharification and co-fermentation |
| CN104073534A (en) * | 2014-07-04 | 2014-10-01 | 天津大学 | Method for reducing dosage of cellulose hydrolase in simultaneous saccharification and cofermentation |
| CN104789605A (en) * | 2015-02-10 | 2015-07-22 | 天津大学 | Method for fermentation production of ethanol in presence of inhibitor |
| CN104561132B (en) * | 2015-02-15 | 2017-10-27 | 天津大学 | The method that bacterium produces ethanol is mixed in the presence of a kind of inhibitor |
| CN104561132A (en) * | 2015-02-15 | 2015-04-29 | 天津大学 | Method for producing ethanol from mixed bacteria with inhibitor |
| CN104611379A (en) * | 2015-03-04 | 2015-05-13 | 天津大学 | Microbial ethanol fermentation method |
| CN104611379B (en) * | 2015-03-04 | 2017-12-29 | 天津大学 | A kind of method of microbial fermentation ethanol |
| CN105368731A (en) * | 2015-11-10 | 2016-03-02 | 四川大学 | Saccharomyces cerevisiae strain for expressing xylose isomerase and construction method |
| CN105368731B (en) * | 2015-11-10 | 2020-08-04 | 四川大学 | Saccharomyces cerevisiae strain for expressing xylose isomerase and construction method thereof |
| CN105368730B (en) * | 2015-11-10 | 2019-01-04 | 四川大学 | The Wine brewing yeast strain and construction method of one plant of Rapid Fermentation xylose producing and ethanol |
| CN105368730A (en) * | 2015-11-10 | 2016-03-02 | 四川大学 | Saccharomyces cerevisiae strain for producing ethanol by quick fermentation of xylose and construction method |
| CN105483033B (en) * | 2015-12-25 | 2019-12-31 | 天津大学 | Bacterial strain, preparation method and application thereof, and fermentation method of bacterial strain |
| CN105483033A (en) * | 2015-12-25 | 2016-04-13 | 天津大学 | Strain as well as preparation method, application and fermentation method thereof |
| CN105567744A (en) * | 2016-01-21 | 2016-05-11 | 天津大学 | Method for improving utilization rate of xylose in lignocellulose hydrolysate |
| CN105567745A (en) * | 2016-01-21 | 2016-05-11 | 天津大学 | Method for improving utilization of xylose in lignocellulose hydrolysate |
| US10759727B2 (en) | 2016-02-19 | 2020-09-01 | Intercontinental Great Brands Llc | Processes to create multiple value streams from biomass sources |
| US11840500B2 (en) | 2016-02-19 | 2023-12-12 | Intercontinental Great Brands Llc | Processes to create multiple value streams from biomass sources |
| CN107916235A (en) * | 2017-11-15 | 2018-04-17 | 天津大学 | A kind of method that restructuring yeast strains and microorganism mix bacterium electricity production |
| CN107916235B (en) * | 2017-11-15 | 2020-12-18 | 天津大学 | Recombinant yeast strain and microorganism mixed strain electricity generation method |
| CN108823113A (en) * | 2018-06-15 | 2018-11-16 | 首都师范大学 | The industrial strain and method of efficient xylose metabolism producing and ethanol |
| CN108823113B (en) * | 2018-06-15 | 2021-12-07 | 首都师范大学 | Industrial bacterial strain and method for producing ethanol by efficient xylose metabolism |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103060217B (en) | 2014-12-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103060217B (en) | Recombinant yeast strain capable of efficiently metabolizing xylose and application thereof | |
| Hu et al. | Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing | |
| Karagöz et al. | Ethanol production from wheat straw by Saccharomyces cerevisiae and Scheffersomyces stipitis co-culture in batch and continuous system | |
| da Cunha-Pereira et al. | Conversion of sugars present in rice hull hydrolysates into ethanol by Spathaspora arborariae, Saccharomyces cerevisiae, and their co-fermentations | |
| Katahira et al. | Ethanol fermentation from lignocellulosic hydrolysate by a recombinant xylose-and cellooligosaccharide-assimilating yeast strain | |
| Koppram et al. | Simultaneous saccharification and co-fermentation for bioethanol production using corncobs at lab, PDU and demo scales | |
| Mishima et al. | Ethanol production from candidate energy crops: water hyacinth (Eichhornia crassipes) and water lettuce (Pistia stratiotes L.) | |
| Zhao et al. | Ethanol production from corn stover hemicellulosic hydrolysate using immobilized recombinant yeast cells | |
| Watanabe et al. | A UV-induced mutant of Pichia stipitis with increased ethanol production from xylose and selection of a spontaneous mutant with increased ethanol tolerance | |
| Yu et al. | Efficient utilization of hemicellulose and cellulose in alkali liquor-pretreated corncob for bioethanol production at high solid loading by Spathaspora passalidarum U1-58 | |
| Li et al. | Bioethanol production from rice straw by a sequential use of Saccharomyces cerevisiae and Pichia stipitis with heat inactivation of Saccharomyces cerevisiae cells prior to xylose fermentation | |
| Hickert et al. | Simultaneous saccharification and co-fermentation of un-detoxified rice hull hydrolysate by Saccharomyces cerevisiae ICV D254 and Spathaspora arborariae NRRL Y-48658 for the production of ethanol and xylitol | |
| Crespo et al. | Ethanol production by continuous fermentation of d-(+)-cellobiose, d-(+)-xylose and sugarcane bagasse hydrolysate using the thermoanaerobe Caloramator boliviensis | |
| CN1966694B (en) | Process for producing alcohol by co-fermentation of glucose and xylose | |
| Bellido et al. | Influence of aeration on bioethanol production from ozonized wheat straw hydrolysates using Pichia stipitis | |
| Zhao et al. | Bioconversion of corn stover hydrolysate to ethanol by a recombinant yeast strain | |
| HRP20161752T1 (en) | Method for preparing an industrial yeast, industrial yeast and use in the production of ethanol from at least one pentose | |
| Tomás-Pejó et al. | Effect of different cellulase dosages on cell viability and ethanol production by Kluyveromyces marxianus in SSF processes | |
| AU2011239830B2 (en) | Industrial yeast capable of producing ethanol from at least one pentose | |
| CN102286538B (en) | Method for producing hydrogen utilizing cellulose | |
| WO2010072093A1 (en) | Method for producing cellulosic ethanol | |
| CN102965291B (en) | Saccharomyces cerevisiae strain and application of strain in production of ethanol by co-fermentation of glucose and xylose | |
| CN102533612B (en) | Clostridium beijerinckii strain and screening method and use thereof | |
| Ren et al. | Anaerobic and sequential aerobic production of high-titer ethanol and single cell protein from NaOH-pretreated corn stover by a genome shuffling-modified Saccharomyces cerevisiae strain | |
| Gao et al. | Efficient ethanol production from inulin by two-stage aerate strategy |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |