CN105555947A - 高表达发酵单胞菌启动子 - Google Patents

高表达发酵单胞菌启动子 Download PDF

Info

Publication number
CN105555947A
CN105555947A CN201480051856.XA CN201480051856A CN105555947A CN 105555947 A CN105555947 A CN 105555947A CN 201480051856 A CN201480051856 A CN 201480051856A CN 105555947 A CN105555947 A CN 105555947A
Authority
CN
China
Prior art keywords
nucleic acid
promoter
acid molecule
zymomonas
glyceraldehyde
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201480051856.XA
Other languages
English (en)
Chinese (zh)
Inventor
J.杨
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of CN105555947A publication Critical patent/CN105555947A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201480051856.XA 2013-09-26 2014-09-25 高表达发酵单胞菌启动子 Pending CN105555947A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14/037,666 US9139818B2 (en) 2013-09-26 2013-09-26 High expression Zymomonas promoters
US14/037666 2013-09-26
PCT/US2014/057386 WO2015048243A1 (en) 2013-09-26 2014-09-25 High expression zymomonas promoters

Publications (1)

Publication Number Publication Date
CN105555947A true CN105555947A (zh) 2016-05-04

Family

ID=51830591

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480051856.XA Pending CN105555947A (zh) 2013-09-26 2014-09-25 高表达发酵单胞菌启动子

Country Status (7)

Country Link
US (1) US9139818B2 (https=)
EP (1) EP3049513A1 (https=)
JP (1) JP2016531555A (https=)
CN (1) CN105555947A (https=)
AU (1) AU2014326594A1 (https=)
BR (1) BR112016006560A2 (https=)
WO (1) WO2015048243A1 (https=)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109852631A (zh) * 2019-02-14 2019-06-07 湖北大学 一种不同强度核糖体结合位点和启动子筛选方法及元件
CN109913487A (zh) * 2019-02-14 2019-06-21 湖北大学 一种基于双荧光报告基因系统鉴定生物元件的方法
WO2020164195A1 (zh) * 2019-02-14 2020-08-20 湖北大学 一种基于双荧光报告基因系统鉴定生物元件的方法及基于该方法构建的生物元件库
CN118652307A (zh) * 2024-06-05 2024-09-17 武汉睿嘉康生物科技有限公司 用于运动发酵单胞菌表面展示的蛋白

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6152127B2 (ja) * 2015-02-16 2017-06-21 日東電工株式会社 偏光子、偏光板および画像表示装置

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101861385A (zh) * 2006-09-28 2010-10-13 纳幕尔杜邦公司 用于乙醇生产的可利用木糖的发酵单胞菌的木糖醇合成突变体
CN101981184A (zh) * 2008-03-27 2011-02-23 纳幕尔杜邦公司 高表达发酵单胞菌属启动子
CN102985540A (zh) * 2010-06-29 2013-03-20 纳幕尔杜邦公司 在具有提高的核糖-5-磷酸活性的重组发酵单胞菌属中的改善的木糖利用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843760A (en) 1994-04-15 1998-12-01 Midwest Research Institute Single zymomonas mobilis strain for xylose and arabinose fermentation
US5726053A (en) 1994-04-15 1998-03-10 Midwest Research Institute Recombinant Zymomonas for pentose fermentation
US5514583A (en) 1994-04-15 1996-05-07 Midwest Research Institute Recombinant zymomonas for pentose fermentation
US5712133A (en) 1994-04-15 1998-01-27 Midwest Research Institute Pentose fermentation by recombinant zymomonas
US7223575B2 (en) 2000-05-01 2007-05-29 Midwest Research Institute Zymomonas pentose-sugar fermenting strains and uses thereof
US6566107B1 (en) 2000-11-01 2003-05-20 Midwest Research Institute Recombinant Zymomonas mobilis with improved xylose utilization
JP2011515107A (ja) 2008-03-27 2011-05-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 改善されたキシロース資化能を有するザイモモナス（Ｚｙｍｏｍｏｎａｓ）
US8623623B2 (en) 2010-06-29 2014-01-07 E I Du Pont De Nemours And Company Xylose utilization in recombinant Zymomonas
US8911983B2 (en) 2011-12-20 2014-12-16 E I Du Pont De Nemours And Company Pnp gene modification for improved xylose utilization in Zymomonas

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101861385A (zh) * 2006-09-28 2010-10-13 纳幕尔杜邦公司 用于乙醇生产的可利用木糖的发酵单胞菌的木糖醇合成突变体
CN101981184A (zh) * 2008-03-27 2011-02-23 纳幕尔杜邦公司 高表达发酵单胞菌属启动子
CN102985540A (zh) * 2010-06-29 2013-03-20 纳幕尔杜邦公司 在具有提高的核糖-5-磷酸活性的重组发酵单胞菌属中的改善的木糖利用

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109852631A (zh) * 2019-02-14 2019-06-07 湖北大学 一种不同强度核糖体结合位点和启动子筛选方法及元件
CN109913487A (zh) * 2019-02-14 2019-06-21 湖北大学 一种基于双荧光报告基因系统鉴定生物元件的方法
WO2020164195A1 (zh) * 2019-02-14 2020-08-20 湖北大学 一种基于双荧光报告基因系统鉴定生物元件的方法及基于该方法构建的生物元件库
US11248258B2 (en) 2019-02-14 2022-02-15 Hubei University Method for characterizing biological part based on dual-fluorescent reporter gene system and biological part library constructed thereon
CN118652307A (zh) * 2024-06-05 2024-09-17 武汉睿嘉康生物科技有限公司 用于运动发酵单胞菌表面展示的蛋白

Also Published As

Publication number Publication date
EP3049513A1 (en) 2016-08-03
US20150087070A1 (en) 2015-03-26
WO2015048243A1 (en) 2015-04-02
AU2014326594A1 (en) 2016-03-10
JP2016531555A (ja) 2016-10-13
US9139818B2 (en) 2015-09-22
BR112016006560A2 (pt) 2017-09-12

Similar Documents

Publication Publication Date Title
JP5444323B2 (ja) 高発現ザイモモナス（Ｚｙｍｏｍｏｎａｓ）プロモーター
CN102037120B (zh) 具有改善的木糖利用率的发酵单胞菌属
ES2385857T3 (es) Zymomonas con producción aumentada de etanol en medio que contiene azúcares concentrados y acetato
BRPI0913547A2 (pt) célula bacteriana ácido-lática recombinante, método para a produção de 2-butanol e método para a produção de 2-butanona
CN110283803A (zh) 一种新型磷脂酶d及其制备磷脂酸、磷脂酰丝氨酸的方法
CN105555947A (zh) 高表达发酵单胞菌启动子
CA2424890C (en) Ethanol production in gram-positive bacteria with a stabilized mutation in lactate dehydrogenase
CN102459570A (zh) 具有改善的阿拉伯糖利用率的发酵单胞菌属细菌
BRPI0807237A2 (pt) Dna que codifica xilitol desidrogenase, construção de ácido nucléico, vetores, microorganismos e métodos para produzir xilitol desigrogenase e etanol
BRPI0709357A2 (pt) ampliação da produção de etanol microbiano
CN103374542B (zh) 一种提高拜氏梭菌木糖消耗率的方法
CN118685338A (zh) 一种重组食气梭菌及其构建方法和应用
CN117625564A (zh) 一种赤藓糖还原酶突变体及其应用
KR101745333B1 (ko) 수크로스에서 중등도 호열성 바실루스의 발효
WO2003016536A2 (en) Plasmids from anaerocellum thermophilum and uses thereof
CN119899250B (zh) 一种淀粉酶高产菌株的构建方法
JP2000350585A (ja) 耐熱耐酸素性ヒドロゲナーゼ遺伝子
WO2017078156A1 (ja) キシロースからエタノールを生産する酵母
CN106459891B (zh) 增强发酵单胞菌细胞中d-木糖和l-阿拉伯糖的利用
HK1143834A (en) Ethanol production
JP2014195442A (ja) 酵母

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20160504

WD01 Invention patent application deemed withdrawn after publication