CN105535116A - Natural medicinal material extract and preparation method thereof - Google Patents
Natural medicinal material extract and preparation method thereof Download PDFInfo
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- A61K2236/30—Extraction of the material
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention discloses a natural medicinal material extract and a preparation method thereof. The extract is prepared from the following components in parts by weight: 42-62 parts of celery seed extract, 15-25 parts of oxygraphis glacialis extract, 6-18 parts of fructus forsythiae extract and 6-18 parts of maca extract. According to the preparation method, celery seed extract, oxygraphis glacialis extract and maca extract which have relatively high purities are obtained by a special process, and are mixed with fructus forsythiae extract in an optimized ratio. The natural medicinal material extract can be used for effectively treating osteoarthritis, and cannot produce drug resistance.
Description
Technical field
The present invention relates to a kind of Chinese medicine extraction mixture, be specifically related to a kind of natural drug extract and preparation method thereof.
Background technology
Knee joint osseous arthritis is also called degenerative osteoarthritis of the knee, be be more common in old people with articular cartilage degenerative change for feature, aseptic, chronic, Progressive symmetric erythrokeratodermia invade sclerotin and to comprise the chronic inflammatory joint comprehensive, at many levels, in various degree of synovial membrane, joint capsule and other structure of joint sick.In the Epidemiological study display crowd of 55 ~ 64 years old, sickness rate reaches 40%, and along with the increase of world aging populations, its sickness rate also rises year by year.
The definite cause of disease of osteoarthritis and pathogeny are illustrated so far not yet completely, and current Therapeutic Method also mainly with alleviating pain, improves function, improve for the purpose of patients ' life quality, not yet have the medicine effecting a radical cure or delay osteoarthritic condition to come out.Western medical treatment is main based on non-steroidal anti-inflammatory drugs at present, but Western medicine price is more expensive, and long-term taking gastrointestinal reaction is large, and gerontal patient's compliance is poor.Be that the TCM Therapy instructed has unique dialectical theory with theory of Chinese medical science, treat this disease with strong points, giving consideration to both the incidental and fundamental, evident in efficacy, and long-term effect is good, can the development of symptom management on the whole, TCM Therapy safety is good, be applicable to long-term treatment, therefore more and more cause the concern of medical circle.
Summary of the invention
For solving the problem, the invention provides a kind of natural drug extract and preparation method thereof, extract obtained osteoarthritis has good therapeutical effect, does not develop immunity to drugs.
For achieving the above object, the technical scheme that the present invention takes is:
A kind of natural drug extract, described extract is prepared from by the component of following weight portion:
Apium graveolens Linnaeus extract 42 ~ 62 parts, Herba oxygraphis glacialis extract 15 ~ 25 parts, Fructus Forsythiae extract 6 ~ 18 parts, Maca extract 6 ~ 18 parts.
Present invention also offers a kind of preparation method of natural drug extract, comprise the steps:
The preparation of S1, Apium graveolens Linnaeus extract extract:
S11, celery seed is pulverized after aqueous solution first carry out membrance separation by composite polrvinyl chloride ultrafilter membrane, collect permeate, then with the NF membrane of molecular cut off 300, extracting solution be concentrated into 10% of original volume, collection concentrated solution;
S12, the concentrated solution obtained is passed through macroporous resin column with the flow velocity of 2 ~ 6BV/h, after dynamic adsorption is saturated, colourless to effluent with the above-mentioned macroporous resin column of flow velocity drip washing of 5 ~ 10BV/h with deionized water, by volume fraction be again 40% ~ 100% organic solvent carry out eluting with the flow velocity of 8 ~ 12BV/h, collect organic solvent eluent, finally will collect gained eluent concentrating under reduced pressure, obtain extractum;
S13, the flow velocity with 3 ~ 5BV/h after extractum water dissolution is passed through polyamide column, after dynamic adsorption is saturated, with 3 ~ 7BV deionized water with the above-mentioned polyamide column of flow velocity drip washing of 2 ~ 10BV/h, by volume fraction be again 20% ~ 100% aqueous solutions of organic solvent carry out eluting with the flow velocity of 5 ~ 10BV/h, collect organic solvent eluent, spraying dry, obtains Apium graveolens Linnaeus extract;
The preparation of S2, Herba oxygraphis glacialis extract:
By the Herba oxygraphis glacialis extract flash extracter process of drying, gained extracting solution first heats with high heat, and with slow fire heating 1 ~ 1.5h after boiling, hollow fiber ultrafiltration membrane is filtered; Medicinal residues add 15 ~ 25 times of soak by water twice, and hollow fiber ultrafiltration membrane is filtered, and merges three filtrates, is concentrated into 1.5g/ml; Slowly add 90 ~ 100% organic solvents, until organic solvent concentration is 55 ~ 65%, cold preservation 8 ~ 9 hours, supernatant is removed after the centrifugal 15 ~ 25min of 2000 ~ 4000r/min, precipitate with deionized water is dissolved to original volume, with organic solvent deposit 2 times, organic solvent concentration is respectively 65 ~ 75%, 75 ~ 85%; Precipitate with deionized water is dissolved to original volume, adds the solution of trichloroacetic acid of 1/6 ~ 1/5 volume 10 ~ 12%, fully leaves standstill 4 ~ 6h after mixing, go to precipitate to obtain supernatant after the centrifugal 15 ~ 25min of 2000 ~ 4000r/min, by gained supernatant sucking filtration, dry, obtain Herba oxygraphis glacialis extract;
The preparation of S3, Maca extract:
S31, be placed in steam-explosion jar by the Lepidinm meyenii Walp of drying, first passing into pressure in nitrogen to steam-explosion jar is 0.7 ~ 1.3MPa, explosion treatment 8 ~ 25min; Then passing into rapidly pressure in steam to steam-explosion jar is 1.3 ~ 1.7MPa, and after Steam explosion treatment 0.5 ~ 2.5min, room temperature is concentrated into relative density 1.10 ~ 1.20, and spraying dry concentrated solution, obtains powder body;
S32, powder body is carried out CO
2supercritical extraction, fluid extraction pressure is 20 ~ 40MPa, extraction temperature 35 ~ 40 DEG C, CO
2fluid flow is 700 ~ 800L/h, and extraction time is 1 ~ 5h, obtains Maca extract;
The preparation of S4, Chinese medicine extraction mixture:
Take Fructus Forsythiae extract 6 ~ 18 parts, the Apium graveolens Linnaeus extract of step S13 gained 42 ~ 62 parts, the Herba oxygraphis glacialis extract of step S2 gained 15 ~ 25 parts, the Maca extract 6 ~ 18 parts of step S32 gained is placed in mixing and blending machine and mixes; By the mixture of gained by the blended pelletize of double screw extruder, obtain finished product.
Preferably, the molecular cut off of the hollow fiber ultrafiltration membrane in described step S2 is 1000.
Preferably, described organic solvent is the turpentine derivatives of natural origin.
Preferably, described turpentine derivatives is isomery, the disproportionation products of pinene.
Preferably, in described step S4, screw extruder rotating speed is 600 ~ 900rpm, and pressure is 2 ~ 3MPa.
The present invention has following beneficial effect:
By special technique, obtain the higher Apium graveolens Linnaeus extract of purity, Herba oxygraphis glacialis extract and Maca extract, and they and Fructus Forsythiae extract are combined by the proportioning of optimum, effectively can treat osteoarthritis, and not develop immunity to drugs.
Detailed description of the invention
In order to make objects and advantages of the present invention clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
In following examples, the molecular cut off of the hollow fiber ultrafiltration membrane used is 1000; Organic solvent is the turpentine derivatives of natural origin; Turpentine derivatives is isomery, the disproportionation products of pinene.
Embodiment 1
The preparation of S1, Apium graveolens Linnaeus extract extract:
S11, celery seed is pulverized after aqueous solution first carry out membrance separation by composite polrvinyl chloride ultrafilter membrane, collect permeate, then with the NF membrane of molecular cut off 300, extracting solution be concentrated into 10% of original volume, collection concentrated solution;
S12, the concentrated solution obtained is passed through macroporous resin column with the flow velocity of 2BV/h, after dynamic adsorption is saturated, colourless to effluent with the above-mentioned macroporous resin column of flow velocity drip washing of 5BV/h with deionized water, by volume fraction be again 40% organic solvent carry out eluting with the flow velocity of 8BV/h, collect organic solvent eluent, finally will collect gained eluent concentrating under reduced pressure, obtain extractum;
S13, the flow velocity with 3BV/h after extractum water dissolution is passed through polyamide column, after dynamic adsorption is saturated, with 3BV deionized water with the above-mentioned polyamide column of flow velocity drip washing of 2BV/h, by volume fraction be again 20% aqueous solutions of organic solvent carry out eluting with the flow velocity of 5BV/h, collect organic solvent eluent, spraying dry, obtains Apium graveolens Linnaeus extract;
The preparation of S2, Herba oxygraphis glacialis extract:
By the Herba oxygraphis glacialis extract flash extracter process of drying, gained extracting solution first heats with high heat, heats 1h after boiling with slow fire, and hollow fiber ultrafiltration membrane is filtered; Medicinal residues add 15 times of soak by water twice, and hollow fiber ultrafiltration membrane is filtered, and merges three filtrates, is concentrated into 1.5g/ml; Slowly add 90% organic solvent, until organic solvent concentration is 55%, cold preservation 8 hours, remove supernatant after the centrifugal 15min of 2000r/min, precipitate with deionized water is dissolved to original volume, and with organic solvent deposit 2 times, organic solvent concentration is respectively 65,75%; Precipitate with deionized water is dissolved to original volume, adds the solution of trichloroacetic acid of 1/6 volume 10%, goes to precipitate to obtain supernatant after fully leaving standstill the centrifugal 15min of 4h, 2000r/min after mixing, by gained supernatant sucking filtration, dries, obtains Herba oxygraphis glacialis extract;
The preparation of S3, Maca extract:
S31, be placed in steam-explosion jar by the Lepidinm meyenii Walp of drying, first passing into pressure in nitrogen to steam-explosion jar is 0.7MPa, explosion treatment 25min; Then passing into rapidly pressure in steam to steam-explosion jar is 1.3MPa, and after Steam explosion treatment 2.5min, room temperature is concentrated into relative density 1.10, and spraying dry concentrated solution, obtains powder body;
S32, powder body is carried out CO
2supercritical extraction, fluid extraction pressure is 20MPa, extraction temperature 35 DEG C, CO
2fluid flow is 700L/h, and extraction time is 1h, obtains Maca extract;
The preparation of S4, Chinese medicine extraction mixture:
Take Fructus Forsythiae extract 6 parts, the Apium graveolens Linnaeus extract of step S13 gained 42 parts, the Herba oxygraphis glacialis extract of step S2 gained 15 parts, the Maca extract 6 parts of step S32 gained is placed in mixing and blending machine and mixes; By the mixture of gained by the blended pelletize of double screw extruder, obtain finished product.
Embodiment 2
The preparation of S1, Apium graveolens Linnaeus extract extract:
S11, celery seed is pulverized after aqueous solution first carry out membrance separation by composite polrvinyl chloride ultrafilter membrane, collect permeate, then with the NF membrane of molecular cut off 300, extracting solution be concentrated into 10% of original volume, collection concentrated solution;
S12, the concentrated solution obtained is passed through macroporous resin column with the flow velocity of 6BV/h, after dynamic adsorption is saturated, colourless to effluent with the above-mentioned macroporous resin column of flow velocity drip washing of 10BV/h with deionized water, by volume fraction be again 100% organic solvent carry out eluting with the flow velocity of 12BV/h, collect organic solvent eluent, finally will collect gained eluent concentrating under reduced pressure, obtain extractum;
S13, the flow velocity with 5BV/h after extractum water dissolution is passed through polyamide column, after dynamic adsorption is saturated, with 7BV deionized water with the above-mentioned polyamide column of flow velocity drip washing of 10BV/h, by volume fraction be again 100% aqueous solutions of organic solvent carry out eluting with the flow velocity of 10BV/h, collect organic solvent eluent, spraying dry, obtains Apium graveolens Linnaeus extract;
The preparation of S2, Herba oxygraphis glacialis extract:
By the Herba oxygraphis glacialis extract flash extracter process of drying, gained extracting solution first heats with high heat, heats 1.5h after boiling with slow fire, and hollow fiber ultrafiltration membrane is filtered; Medicinal residues add 25 times of soak by water twice, and hollow fiber ultrafiltration membrane is filtered, and merges three filtrates, is concentrated into 1.5g/ml; Slowly add 100% organic solvent, until organic solvent concentration is 65%, cold preservation 9 hours, supernatant is removed after the centrifugal 25min of 4000r/min, precipitate with deionized water is dissolved to original volume, and with organic solvent deposit 2 times, organic solvent concentration is respectively 75%, 85%; Precipitate with deionized water is dissolved to original volume, adds the solution of trichloroacetic acid of 1/5 volume 12%, goes to precipitate to obtain supernatant after fully leaving standstill the centrifugal 25min of 6h, 4000r/min after mixing, by gained supernatant sucking filtration, dries, obtains Herba oxygraphis glacialis extract;
The preparation of S3, Maca extract:
S31, be placed in steam-explosion jar by the Lepidinm meyenii Walp of drying, first passing into pressure in nitrogen to steam-explosion jar is 1.3MPa, explosion treatment 8min; Then passing into rapidly pressure in steam to steam-explosion jar is 1.7MPa, and after Steam explosion treatment 0.5min, room temperature is concentrated into relative density 1.20, and spraying dry concentrated solution, obtains powder body;
S32, powder body is carried out CO
2supercritical extraction, fluid extraction pressure is 40MPa, extraction temperature 40 DEG C, CO
2fluid flow is 800L/h, and extraction time is 5h, obtains Maca extract;
The preparation of S4, Chinese medicine extraction mixture:
Take Fructus Forsythiae extract 18 parts, the Apium graveolens Linnaeus extract of step S13 gained 62 parts, the Herba oxygraphis glacialis extract of step S2 gained 25 parts, the Maca extract 18 parts of step S32 gained is placed in mixing and blending machine and mixes; By the mixture of gained by the blended pelletize of double screw extruder, obtain finished product.
Embodiment 3
The preparation of S1, Apium graveolens Linnaeus extract extract:
S11, celery seed is pulverized after aqueous solution first carry out membrance separation by composite polrvinyl chloride ultrafilter membrane, collect permeate, then with the NF membrane of molecular cut off 300, extracting solution be concentrated into 10% of original volume, collection concentrated solution;
S12, the concentrated solution obtained is passed through macroporous resin column with the flow velocity of 4BV/h, after dynamic adsorption is saturated, colourless to effluent with the above-mentioned macroporous resin column of flow velocity drip washing of 7.5BV/h with deionized water, by volume fraction be again 70% organic solvent carry out eluting with the flow velocity of 10BV/h, collect organic solvent eluent, finally will collect gained eluent concentrating under reduced pressure, obtain extractum;
S13, the flow velocity with 4BV/h after extractum water dissolution is passed through polyamide column, after dynamic adsorption is saturated, with 5BV deionized water with the above-mentioned polyamide column of flow velocity drip washing of 6BV/h, by volume fraction be again 60% aqueous solutions of organic solvent carry out eluting with the flow velocity of 7.5BV/h, collect organic solvent eluent, spraying dry, obtains Apium graveolens Linnaeus extract;
The preparation of S2, Herba oxygraphis glacialis extract:
By the Herba oxygraphis glacialis extract flash extracter process of drying, gained extracting solution first heats with high heat, heats 1.25h after boiling with slow fire, and hollow fiber ultrafiltration membrane is filtered; Medicinal residues add 20 times of soak by water twice, and hollow fiber ultrafiltration membrane is filtered, and merges three filtrates, is concentrated into 1.5g/ml; Slowly add 90 ~ 100% organic solvents, until organic solvent concentration is 60%, cold preservation 8.5 hours, supernatant is removed after the centrifugal 20min of 3000r/min, precipitate with deionized water is dissolved to original volume, and with organic solvent deposit 2 times, organic solvent concentration is respectively 65 ~ 75%, 75 ~ 85%; Precipitate with deionized water is dissolved to original volume, adds the solution of trichloroacetic acid of 11/60 volume 11%, goes to precipitate to obtain supernatant after fully leaving standstill the centrifugal 20min of 5h, 3000r/min after mixing, by gained supernatant sucking filtration, dries, obtains Herba oxygraphis glacialis extract;
The preparation of S3, Maca extract:
S31, be placed in steam-explosion jar by the Lepidinm meyenii Walp of drying, first passing into pressure in nitrogen to steam-explosion jar is 1MPa, explosion treatment 16.5min; Then passing into rapidly pressure in steam to steam-explosion jar is 1.5MPa, and after Steam explosion treatment 1.5min, room temperature is concentrated into relative density 1.15, and spraying dry concentrated solution, obtains powder body;
S32, powder body is carried out CO
2supercritical extraction, fluid extraction pressure is 30MPa, extraction temperature 37.5 DEG C, CO
2fluid flow is 750L/h, and extraction time is 3h, obtains Maca extract;
The preparation of S4, Chinese medicine extraction mixture:
Take Fructus Forsythiae extract 12 parts, the Apium graveolens Linnaeus extract of step S13 gained 52 parts, the Herba oxygraphis glacialis extract of step S2 gained 20 parts, the Maca extract 12 parts of step S32 gained is placed in mixing and blending machine and mixes; By the mixture of gained by the blended pelletize of double screw extruder, obtain finished product.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (6)
1. a natural drug extract, is characterized in that, described extract is prepared from by the component of following weight portion:
Apium graveolens Linnaeus extract 42 ~ 62 parts, Herba oxygraphis glacialis extract 15 ~ 25 parts, Fructus Forsythiae extract 6 ~ 18 parts, Maca extract 6 ~ 18 parts.
2. a preparation method for natural drug extract, is characterized in that, comprises the steps:
The preparation of S1, Apium graveolens Linnaeus extract extract:
S11, celery seed is pulverized after aqueous solution first carry out membrance separation by composite polrvinyl chloride ultrafilter membrane, collect permeate, then with the NF membrane of molecular cut off 300, extracting solution be concentrated into 10% of original volume, collection concentrated solution;
S12, the concentrated solution obtained is passed through macroporous resin column with the flow velocity of 2 ~ 6BV/h, after dynamic adsorption is saturated, colourless to effluent with the above-mentioned macroporous resin column of flow velocity drip washing of 5 ~ 10BV/h with deionized water, by volume fraction be again 40% ~ 100% organic solvent carry out eluting with the flow velocity of 8 ~ 12BV/h, collect organic solvent eluent, finally will collect gained eluent concentrating under reduced pressure, obtain extractum;
S13, the flow velocity with 3 ~ 5BV/h after extractum water dissolution is passed through polyamide column, after dynamic adsorption is saturated, with 3 ~ 7BV deionized water with the above-mentioned polyamide column of flow velocity drip washing of 2 ~ 10BV/h, by volume fraction be again 20% ~ 100% aqueous solutions of organic solvent carry out eluting with the flow velocity of 5 ~ 10BV/h, collect organic solvent eluent, spraying dry, obtains Apium graveolens Linnaeus extract;
The preparation of S2, Herba oxygraphis glacialis extract:
By the Herba oxygraphis glacialis extract flash extracter process of drying, gained extracting solution first heats with high heat, and with slow fire heating 1 ~ 1.5h after boiling, hollow fiber ultrafiltration membrane is filtered; Medicinal residues add 15 ~ 25 times of soak by water twice, and hollow fiber ultrafiltration membrane is filtered, and merges three filtrates, is concentrated into 1.5g/ml; Slowly add 90 ~ 100% organic solvents, until organic solvent concentration is 55 ~ 65%, cold preservation 8 ~ 9 hours, supernatant is removed after the centrifugal 15 ~ 25min of 2000 ~ 4000r/min, precipitate with deionized water is dissolved to original volume, with organic solvent deposit 2 times, organic solvent concentration is respectively 65 ~ 75%, 75 ~ 85%; Precipitate with deionized water is dissolved to original volume, adds the solution of trichloroacetic acid of 1/6 ~ 1/5 volume 10 ~ 12%, fully leaves standstill 4 ~ 6h after mixing, go to precipitate to obtain supernatant after the centrifugal 15 ~ 25min of 2000 ~ 4000r/min, by gained supernatant sucking filtration, dry, obtain Herba oxygraphis glacialis extract;
The preparation of S3, Maca extract:
S31, be placed in steam-explosion jar by the Lepidinm meyenii Walp of drying, first passing into pressure in nitrogen to steam-explosion jar is 0.7 ~ 1.3MPa, explosion treatment 8 ~ 25min; Then passing into rapidly pressure in steam to steam-explosion jar is 1.3 ~ 1.7MPa, and after Steam explosion treatment 0.5 ~ 2.5min, room temperature is concentrated into relative density 1.10 ~ 1.20, and spraying dry concentrated solution, obtains powder body;
S32, powder body is carried out CO
2supercritical extraction, fluid extraction pressure is 20 ~ 40MPa, extraction temperature 35 ~ 40 DEG C, CO
2fluid flow is 700 ~ 800L/h, and extraction time is 1 ~ 5h, obtains Maca extract;
The preparation of S4, Chinese medicine extraction mixture:
Take Fructus Forsythiae extract 6 ~ 18 parts, the Apium graveolens Linnaeus extract of step S13 gained 42 ~ 62 parts, the Herba oxygraphis glacialis extract of step S2 gained 15 ~ 25 parts, the Maca extract 6 ~ 18 parts of step S32 gained is placed in mixing and blending machine and mixes; By the mixture of gained by the blended pelletize of double screw extruder, obtain finished product.
3. the preparation method of a kind of natural drug extract according to claim 2, is characterized in that, the molecular cut off of the hollow fiber ultrafiltration membrane in described step S2 is 1000.
4. the preparation method of a kind of natural drug extract according to claim 2, is characterized in that, described organic solvent is the turpentine derivatives of natural origin.
5. the preparation method of a kind of natural drug extract according to claim 4, is characterized in that, described turpentine derivatives is isomery, the disproportionation products of pinene.
6. the preparation method of a kind of natural drug extract according to claim 2, is characterized in that, in described step S4, screw extruder rotating speed is 600 ~ 900rpm, and pressure is 2 ~ 3MPa.
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Cited By (6)
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| CN105796892A (en) * | 2016-05-27 | 2016-07-27 | 陕西国际商贸学院 | Traditional Chinese medicine extractive composition and preparation method thereof |
| CN105833006A (en) * | 2016-05-20 | 2016-08-10 | 宁夏医科大学总医院 | Traditional Chinese medicine substituting tea brewing drink for treating acute and chronic faucitis |
| CN106135315A (en) * | 2016-06-29 | 2016-11-23 | 辽宁科技学院 | A kind of natural botanical insecticide |
| CN106334044A (en) * | 2016-08-16 | 2017-01-18 | 西安市第医院 | Medicament capable of improving central nervous system inflammation |
| CN108671067A (en) * | 2018-07-27 | 2018-10-19 | 吉林大学珠海学院 | A kind of oxidation resistant compound Chinese medicine extraction isolate and preparation method thereof |
| CN115720954A (en) * | 2022-12-02 | 2023-03-03 | 刘晓军 | Chewing gum containing salt and preparation method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105833006A (en) * | 2016-05-20 | 2016-08-10 | 宁夏医科大学总医院 | Traditional Chinese medicine substituting tea brewing drink for treating acute and chronic faucitis |
| CN105796892A (en) * | 2016-05-27 | 2016-07-27 | 陕西国际商贸学院 | Traditional Chinese medicine extractive composition and preparation method thereof |
| CN106135315A (en) * | 2016-06-29 | 2016-11-23 | 辽宁科技学院 | A kind of natural botanical insecticide |
| CN106334044A (en) * | 2016-08-16 | 2017-01-18 | 西安市第医院 | Medicament capable of improving central nervous system inflammation |
| CN108671067A (en) * | 2018-07-27 | 2018-10-19 | 吉林大学珠海学院 | A kind of oxidation resistant compound Chinese medicine extraction isolate and preparation method thereof |
| CN115720954A (en) * | 2022-12-02 | 2023-03-03 | 刘晓军 | Chewing gum containing salt and preparation method thereof |
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