CN105532534A - Cyst removal method for controlling fish cryptocaryon irritans - Google Patents

Cyst removal method for controlling fish cryptocaryon irritans Download PDF

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Publication number
CN105532534A
CN105532534A CN201511007343.1A CN201511007343A CN105532534A CN 105532534 A CN105532534 A CN 105532534A CN 201511007343 A CN201511007343 A CN 201511007343A CN 105532534 A CN105532534 A CN 105532534A
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CN
China
Prior art keywords
fish
shield
pond
culturing pool
cryptocaryon irritans
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CN201511007343.1A
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Chinese (zh)
Inventor
李安兴
江飚
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Sun Yat Sen University
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Sun Yat Sen University
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Priority to CN201511007343.1A priority Critical patent/CN105532534A/en
Publication of CN105532534A publication Critical patent/CN105532534A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention discloses a cyst removal method for controlling fish cryptocaryon irritans. A cloth liner with the strong attaching effect on cryptocaryon irritans brown cysts is screened out mainly and laid on a water bottom of fish infected with the cryptocaryon irritans, the cryptocaryon irritans brown is nourished, falls down and is attached to the cloth liner, the cryptocaryon irritans brown cysts are removed by removing the cloth liner, the life history of the cryptocaryon irritans brown is cut off, and deaths caused when fish is repeatedly infected with the cryptocaryon irritans brown are reduced. By screening the materials, the cloth liner which is resistant to corrosion and free of toxicity and has the strong attaching effect on the cryptocaryon irritans brown cysts is obtained, the method for removing the cryptocaryon irritans brown cysts is used for treating plectorhinchus cinctus infected with cryptocaryon irritans, and the relative protection rate can reach 98%.

Description

A kind of packing removing method controlling fish stimulation cryptocaryoniosis
Technical field
The invention belongs to fish disease prevention and control field, be specifically related to a kind of packing removing method controlling fish stimulation cryptocaryoniosis.
Background technology
The present invention has very strong adhesiveness according to stimulation cryptonucleus insect packing, packing causes take three day time less from being formed into larva effusion, shield is laid bottom the fishpond of infecting stimulation cryptonucleus insect, periodic replacement, cuts off its history of life, to reach the object controlling to stimulate cryptocaryoniosis.
Cryptonucleus insect is stimulated to be a kind of infusorian extensively parasitizing the torrid zone, subtropics Marine teleost, due to the infected body surface of fish body, fin ray, the gill there will be macroscopic white point, therefore named " ichthyophthirius ".Stimulate cryptonucleus insect there is no intermediate host, be divided into host's parasitic stage and free living phase its history of life, experience 4 polypides abnormal stage, be respectively: trophozoite, packing precursor, cyst and larva.Trophozoite vegetative period is 3 ~ 7d, host is departed from after maturation, form freely movable packing early stage, adhere on water-bed wall after water-bed " creeping " 2 ~ 8h, in 8 ~ 12h, form hard packing, packing experiences the asymmetric binary fission of 3 ~ 28d usually, finally form larva to hatch out from packing, a packing can hatch about 200 larvas, and larva effusion is travelling fast in water to be found and infection host, thus enters next life cycle.
In recent years, along with seawater fishery intensive culture degree improves gradually, cultivation density increases, and breeding environment runs down, and stimulate the harm of cryptocaryoniosis day by day serious, but effectively control is short of still very much.At present to the theoretical research aspect stimulating the research of cryptonucleus insect mainly to concentrate on etiology, ecology, immunology etc., Hirazawa etc. find significantly reduce quantity sparid body stimulating cryptonucleus insect trophozoite ocean temperature 17 DEG C of medium chain fatty acids of throwing something and feeding (sad), but water temperature reaches 24 DEG C just loses effect.There is expert to study the Chinese herbal medicines such as capsicum, ginger, gall nut, betel nut to the preventive and therapeutic effect stimulating cryptonucleus insect, get certain achievement, but production and application cost is high, be not suitable for using during large-scale farming is produced.Production also adopts some physical control methods comprise freshwater soaking, thermal treatment, drying, rotation cultivation, use ultraviolet and ozone treatment breeding water body etc., these methods serve certain preventive and therapeutic effect, but have shortcoming in experimental implementation.Therefore the method that prevention and therapy stimulates cryptocaryoniosis conventional is at present still frequently use the chemicalses such as formaldehyde, CuSO4, copper iron mixture, although chemicals effectively controls to stimulate cryptocaryoniosis, but there is huge hidden danger in practical application, as the toxicity to fish, medicament residue, contaminated environment etc., therefore a kind of methods for the treatment of of environmental protection is extremely urgent.
Summary of the invention
The present invention includes a kind of packing removing method controlling fish stimulation cryptocaryoniosis.
The present invention is by screening packing adhesiveness, corrosion-resistant and break resistance the cloth of 12 kinds of unlike materials, comprise: composite polyethylene colour bar cloth, PVC plastic-coated waterproof cloth, organic silicon cloth, PE plastic-coated waterproof cloth, oxford, pure cotton canvas, linen canvas, polypropylene spunbond non-woven fabrics, silk ribbon fall canopy cloth, the transparent tablecloth of PVC waterproof soft glass, soft grenadine cloth, finishing screen is selected silk ribbon and to be fallen canopy cloth (see Fig. 1), this cloth surface has can allow the groove of packing firm attachment, anticorrosion, to fish nonhazardous effect.By laying shield bottom the breeding barrel infecting stimulation cryptonucleus insect or culturing pool, within every 3 days, changing once, changing 4 times altogether, verify that its control stimulates the effect of cryptocaryoniosis.
Concrete technical scheme comprises the steps: 1, the preparation of culturing pool and shield
Culturing pool is the indoor water mud sump of batch production marine fish culture, and length, width and height are 8m × 4m × 2m, smooth at the bottom of pond, fishpond; The canopy cloth that shield selects 450D × 450D100% dacron thread woven, radial fragmentation intensity is greater than 1500N, and broadwise is greater than 1000N, thickness 0.5mm; Shield is made size and form at the bottom of pond, fishpond, length and width are 8m × 4m, each steel pipe fixing a diameter 4cm, long 4m in shield two ends, and connect two diameter 0.4cm, long 12m nylon rope on the both sides of two ends steel pipe.
2, fish disease qualification and sick fish enter pond
Fish disease is identified: in stimulation cryptocaryoniosis suffering season May to October, when water temperature is greater than 24 DEG C, observe that fish are no has following symptom: fish presents abnormal travelling, poor appetite, often and at the bottom of pond, pool wall rubs, percussion, and belly and fin have scar, expiratory dyspnea, the mucus of body surface and the gill increases, and has " new line " to have phenomenon in fishpond, and white point appears in fish body body surface.After finding above symptom, the obvious sick fish of selective body vindication point, eugenol is utilized to anaesthetize, concentration is 40-60mg/L, anesthesia level makes fish inertia be good, gather the gill of fish of dying of illness, fin or scraping epidermal mucus to be placed on slide, if or oval agglomerate circular in black can be observed under the microscope and the trophozoite rotated, can make a definite diagnosis.
Sick fish enters pond: be diagnosed as stimulate cryptocaryoniosis fishpond water level lower, utilize eugenol to anaesthetize, concentration is 20-40mg/L, and anesthesia level makes fish disequilibrium be good; After anesthesia, rapidly sick fish is transferred in the culturing pool for the treatment of.
3, packing is removed
After sick fish moves into the culturing pool for the treatment of, at the bottom of culturing pool pond, lay shield, one end shield being fixed steel pipe is put in culturing pool, utilizes rope slowly to drag steel pipe, makes shield be paved with at the bottom of pond, and shield surrounding diameter 4cm, long 4m steel pipe are fixed; Within every 3 days, change a shield, slowly drag rope during replacing and shield is shifted out in culturing pool, change new shield; Until fish body body surface white point disappears, travelling, appetite normal, observe without polypide through microscopy, fish can be retracted former pond and normally cultivate.
Embodiment
Embodiment one: by removing the effect stimulating cryptonucleus insect encapsulation process to treat this disease
This experiment Plectorhinchus cinctus (27.79 ± 3.07g/ tail) is purchased from Ningde City San Du Ao bight.Before experiment in the round plastic bucket (volume is 1 ton) handling two weeks, the raising of miniflow water, particulate material of throwing something and feeding, seawater adopts natural sea-water, salinity 29 ~ 32 ‰, dissolved oxygen >=5.0mg/l, pH7.9 ~ 8.3, nitrite < 0.1mgN/l.
Plectorhinchus cinctus is divided into three groups at random: control group, infected group, shield group, often organizes three repeating groups, every repeating groups 30 tail fish.Wherein infected group, shield group infect 30 tail Plectorhinchus cinctus with 3000 larvas/fish, and seawater consumption is 5L/ fish, open flowing water after infecting 2h.Control group, infected group normally cultivate, shield group adopts and is tested by the woven canopy cloth made of terylen, first paulin is made round plastic bucket Bottom Shape, at the bottom of fish infects and stimulates cryptonucleus insect within second day, to be layed in barrel, within every 3 days, replace with new paulin, totally 4 times, it is for subsequent use that the paulin removed carries out natural seasoning process.Add up the lethality of each group in two weeks, result shows, the lethality of control group, infected group, shield group is respectively: 0%, 100%, 2%, stimulates the method for cryptonucleus insect packing can effectively control to stimulate cryptocaryoniosis by a kind of removing.
Embodiment two:
1, the preparation of culturing pool and shield
Culturing pool is the indoor water mud sump of batch production marine fish culture, and length, width and height are 8m × 4m × 2m, smooth at the bottom of pond, fishpond; The canopy cloth that shield selects 450D × 450D100% dacron thread woven, radial fragmentation intensity is greater than 1500N, and broadwise is greater than 1000N, thickness 0.5mm; Shield is made size and form at the bottom of pond, fishpond, length and width are 8m × 4m, each steel pipe fixing a diameter 4cm, long 4m in shield two ends, and connect two diameter 0.4cm, long 12m nylon rope on the both sides of two ends steel pipe.
2, fish disease qualification and sick fish enter pond
Fish disease is identified: in stimulation cryptocaryoniosis suffering season May to October, when water temperature is greater than 24 DEG C, observe that fish are no has following symptom: fish presents abnormal travelling, poor appetite, often and at the bottom of pond, pool wall rubs, percussion, and belly and fin have scar, expiratory dyspnea, the mucus of body surface and the gill increases, and has " new line " to have phenomenon in fishpond, and white point appears in fish body body surface.After finding above symptom, the obvious sick fish of selective body vindication point, eugenol is utilized to anaesthetize, concentration is 40-60mg/L, anesthesia level makes fish inertia be good, gather the gill of fish of dying of illness, fin or scraping epidermal mucus to be placed on slide, if or oval agglomerate circular in black can be observed under the microscope and the trophozoite rotated, can make a definite diagnosis.
Sick fish enters pond: be diagnosed as stimulate cryptocaryoniosis fishpond water level lower, utilize eugenol to anaesthetize, concentration is 20-40mg/L, and anesthesia level makes fish disequilibrium be good; After anesthesia, rapidly sick fish is transferred in the culturing pool for the treatment of.
3, packing is removed
After sick fish moves into the culturing pool for the treatment of, at the bottom of culturing pool pond, lay shield, one end shield being fixed steel pipe is put in culturing pool, utilizes rope slowly to drag steel pipe, makes shield be paved with at the bottom of pond, and shield surrounding diameter 4cm, long 4m steel pipe are fixed; Within every 3 days, change a shield, slowly drag rope during replacing and shield is shifted out in culturing pool, change new shield; Until fish body body surface white point disappears, travelling, appetite normal, observe without polypide through microscopy, fish can be retracted former pond and normally cultivate.
Accompanying drawing explanation
Fig. 1 is attached to the stimulation cryptonucleus insect packing of shield.

Claims (1)

1. control the packing removing method that fish stimulate cryptocaryoniosis, the method comprises the steps:
(1) preparation of culturing pool and shield
Culturing pool is the indoor water mud sump of batch production marine fish culture, and length, width and height are 8m × 4m × 2m, smooth at the bottom of pond, fishpond; The canopy cloth that shield selects 450D × 450D100% dacron thread woven, radial fragmentation intensity is greater than 1500N, and broadwise is greater than 1000N, thickness 0.5mm; Shield is made size and form at the bottom of pond, fishpond, length and width are 8m × 4m, each steel pipe fixing a diameter 4cm, long 4m in shield two ends, and connect two diameter 0.4cm, long 12m nylon rope on the both sides of two ends steel pipe.
(2) fish disease qualification and sick fish enter pond
Fish disease is identified: in stimulation cryptocaryoniosis suffering season May to October, when water temperature is greater than 24 DEG C, observe that fish are no has following symptom: fish presents abnormal travelling, poor appetite, often and at the bottom of pond, pool wall rubs, percussion, and belly and fin have scar, expiratory dyspnea, the mucus of body surface and the gill increases, and has " new line " to have phenomenon in fishpond, and white point appears in fish body body surface.After finding above symptom, the obvious sick fish of selective body vindication point, eugenol is utilized to anaesthetize, concentration is 40-60mg/L, anesthesia level makes fish inertia be good, gather the gill of fish, fin or scraping epidermal mucus to be placed on slide, if or oval agglomerate circular in black can be observed under the microscope and the trophozoite rotated, can make a definite diagnosis.
Sick fish enters pond: be diagnosed as stimulate cryptocaryoniosis fishpond water level lower, utilize eugenol to anaesthetize, concentration is 20-40mg/L, and anesthesia level makes fish disequilibrium be good; After anesthesia, rapidly sick fish is transferred in the culturing pool for the treatment of.
(3) packing is removed
After sick fish moves into the culturing pool for the treatment of, at the bottom of culturing pool pond, lay shield, one end shield being fixed steel pipe is put in culturing pool, utilizes rope slowly to drag steel pipe, makes shield be paved with at the bottom of pond, and shield surrounding diameter 4cm, long 4m steel pipe are fixed; Within every 3 days, change a shield, slowly drag rope during replacing and shield is shifted out in culturing pool, change new shield; Until fish body body surface white point disappears, travelling, appetite normal, observe without polypide through microscopy, fish can be retracted former pond and normally cultivate.
CN201511007343.1A 2015-12-18 2015-12-18 Cyst removal method for controlling fish cryptocaryon irritans Pending CN105532534A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111742870A (en) * 2020-07-06 2020-10-09 宁德市富发水产有限公司 Novel multifunctional indoor large yellow croaker culture pond and use method thereof
CN112889717A (en) * 2021-02-24 2021-06-04 中山大学 Method for biologically preventing and controlling cryptocaryon irritans infection by using tilapia

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4677118A (en) * 1985-10-01 1987-06-30 Elena Avram Composition and method for treatment of copper deficiency
CN102670649A (en) * 2011-12-22 2012-09-19 河南科技大学 Pesticide for preventing and treating ichthyophthiriasis of pet fish
CN102726327A (en) * 2012-06-20 2012-10-17 中国水产科学研究院东海水产研究所 Method for removing residual cryptocryon irritans from culture pond in pollution-free way
CN103623068A (en) * 2013-11-13 2014-03-12 江西省水产科学研究所 Drug for treating ichthyophthiriasis in fish
CN204032119U (en) * 2014-08-27 2014-12-24 中国水产科学研究院东海水产研究所 A kind of removal device cultivating fish body stimulation cryptonucleus insect

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4677118A (en) * 1985-10-01 1987-06-30 Elena Avram Composition and method for treatment of copper deficiency
CN102670649A (en) * 2011-12-22 2012-09-19 河南科技大学 Pesticide for preventing and treating ichthyophthiriasis of pet fish
CN102726327A (en) * 2012-06-20 2012-10-17 中国水产科学研究院东海水产研究所 Method for removing residual cryptocryon irritans from culture pond in pollution-free way
CN103623068A (en) * 2013-11-13 2014-03-12 江西省水产科学研究所 Drug for treating ichthyophthiriasis in fish
CN204032119U (en) * 2014-08-27 2014-12-24 中国水产科学研究院东海水产研究所 A kind of removal device cultivating fish body stimulation cryptonucleus insect

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
于天飞等: "鲆鲽类等鱼类刺激隐核虫病的防治", 《科学养鱼》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111742870A (en) * 2020-07-06 2020-10-09 宁德市富发水产有限公司 Novel multifunctional indoor large yellow croaker culture pond and use method thereof
CN111742870B (en) * 2020-07-06 2022-08-12 宁德市富发水产有限公司 Novel multifunctional indoor large yellow croaker culture pond and use method thereof
CN112889717A (en) * 2021-02-24 2021-06-04 中山大学 Method for biologically preventing and controlling cryptocaryon irritans infection by using tilapia

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