CN105521020B

CN105521020B - Plant extract composition with human body dermatosis controlling activity

Info

Publication number: CN105521020B
Application number: CN201510796884.0A
Authority: CN
Inventors: 刘小锦
Original assignee: Anhui Normal University
Current assignee: Shanghai Tianwangyeng Biotechnology Co ltd
Priority date: 2015-11-17
Filing date: 2015-11-17
Publication date: 2021-06-18
Anticipated expiration: 2035-11-17
Also published as: CN105521020A

Abstract

The invention relates to a plant extract composition with human body skin disease control activity, which comprises the following raw materials in percentage by weight: 3-50% of vegetable oil fatty acid or 1-65% of vegetable essential oil, 0.1-0.5% of antioxidant and 10-30% of surfactant. The invention uses one or more vegetable oils of litsea cubeba oil, peppermint oil, citral, eucalyptus oil, citronella oil, thyme oil, clove oil, wintergreen oil, rosemary oil, rape oil and coconut oil, and has strong inhibitory activity on fungi such as malassezia furfur, trichophyton rubrum, candida albicans and the like which cause human body dermatosis.

Description

Plant extract composition with human body dermatosis controlling activity

Technical Field

The invention relates to the field of human body skin disease prevention and treatment, in particular to a plant extract composition with human body skin disease control activity.

Background

Malassezia (Pityrosporum), also known as Pityrosporum, is mycologically classified as a hyphomycete, belonging to the order Basidiomycota-Basidiomycetes. The malassezia furfur contains lipase, which exists in a cell wall and/or a cell membrane system rich in glucan in a mosaic mode, decomposes lipid into fatty acid to provide necessary nutrient sources through self metabolism, so that the malassezia furfur is mainly parasitic on abundant parts of sebaceous glands of a human body, such as chest, back, head, face, neck and the like, and is a conditional pathogen. The high risk group comprises newborn with cardiopulmonary disease, severe gastrointestinal disease patients, children with low immunity and adults.

Malassezia spp is a conditionally pathogenic fungus on the skin surface of human bodies and other warm-blooded animals, and diseases related to the malassezia spp comprise pityriasis versicolor, Malassezia spp folliculitis, seborrheic dermatitis, atopic dermatitis and the like, and the treatment of the malassezia spp is also increasingly emphasized due to the fact that the malassezia spp relates to a large number of diseases.

In recent years, infections caused by dermatophytes and other fungi have increased dramatically. More than 40 species of dermatophytes are currently found, of which more than 30 species are pathogenic. The dermatophytes which often cause infection mainly comprise trichophyton rubrum, trichophyton mentagrophytes, microsporum canis and the like. Trichophyton rubrum (Trichophyton rubrum) belongs to mitosporic fungi, is one of the most common parent pathogenic dermatophytes, and is also one of the main pathogenic bacteria of superficial mycosis. It is distributed worldwide and is also the most common dermatophyte in China. Trichophyton rubrum can cause skin and hair infections; and often cause chronic refractory infections of the foot and nails. Clinically, more than half of tinea corporis and more than 90% of tinea cruris and tinea manus and pedis are caused by trichophyton rubrum. The clinical positive rate of the isolation culture of the strain can reach 67 percent, and the peak of infection attack is in spring and summer.

Candida albicans is a unicellular fungus. Round or oval, positive gram staining. The germinated seeds are called blastospores. The spore extends into a germ tube and does not separate from the parent body, and long pseudohypha is formed. Candida albicans conditioned pathogenic bacteria usually exist on the body surface and in the cavity of a human body, when normal flora is disordered or the resistance is reduced, the Candida albicans can invade a plurality of parts of the human body, such as skin, mucosa, lung, intestine, kidney and brain, and cause skin mucosa infection (common thrush, vulvitis and vaginitis), visceral infection, central nervous system infection and the like, and the mucosal infection is the most frequently thrush.

At present, aiming at the three common skin pathogenic bacteria, the three common skin pathogenic bacteria are mostly treated by pure western medicines, the types of the medicines are limited, and the medicines are easy to repeat, so that the development of a safe and effective pure plant preparation becomes a research hotspot in the aspect of treatment.

Disclosure of Invention

The present invention aims at overcoming the defects of the prior art and providing a plant extract composition with human body skin disease control activity.

The invention provides a plant extract composition with human body skin disease control activity, which comprises the following raw materials in percentage by weight: 3-50% of vegetable oil fatty acid or 1-65% of vegetable essential oil, 0.1-0.5% of antioxidant and 10-30% of surfactant.

Wherein the vegetable oil is one or more of fructus Litseae oil, oleum Menthae Dementholatum, citral, oleum Eucalypti, citronella oil, thyme oil, oleum Caryophylli, wintergreen oil, Rosemary oil, rape oil, and oleum Cocois.

Wherein the antioxidant is one or more of tert-butyl hydroquinone (TBHQ), Butyl Hydroxy Anisol (BHA) and 2, 6-di-tert-butyl-p-cresol (BHT).

Wherein, the surfactant is one or two of Dofax111 and 111.

Compared with the existing preparation, the invention uses one or more vegetable oils of litsea cubeba oil, peppermint oil, citral, eucalyptus oil, citronella oil, thyme oil, clove oil, wintergreen oil, rosemary oil, rape oil and coconut oil, and has strong inhibitory activity on fungi such as malassezia furfur, trichophyton rubrum, candida albicans and the like which cause human body skin diseases; the surfactant can make the vegetable oil uniformly disperse in water to form stable microemulsion, and the solvent can dilute. The solvent used in the invention is a low VOC solvent, and is harmless to the ecological environment.

Detailed Description

The following examples further illustrate specific embodiments of the present invention. The embodiment is used to more clearly illustrate the technical solution of the present invention, and the protection scope of the present invention is not limited thereby.

The composition of the invention comprises the following preparation combinations: (in weight percent)

Tests were carried out with the above preparations to investigate their control of malassezia furfur, trichophyton rubrum, candida albicans:

materials: the experimental pityrosporum malayanum, trichophyton rubrum and candida albicans are activated and grow for 2 days. The concentration gradient of the litsea cubeba oil, peppermint oil, citral, eucalyptus oil, citronella oil, thyme oil, clove oil, wintergreen oil, rosemary oil, rape oil, coconut oil and cinnamaldehyde preparations is designed, the MIC value of the preparations is measured by a 96-well plate observation method, and three preparations are arranged in parallel in each group.

Results and analysis:

MIC of plant extract preparation against three dermatophytes.

The experiment prepares twelve plant extract preparations which have good sterilization effects on common pathogenic bacteria of human bodies, namely malarial ringworm fungus, trichophyton rubrum and candida albicans.

Through HPLC-GC mass analysis, the main components of the litsea cubeba oil are geranial (71.7 percent) and cyclohexanol (15.1 percent), and a small amount of geraniol, heteroxobicycloheptane, 2-hexenal, caryophyllene and the like;

the main components of eucalyptus oil are eucalyptol (78.55%) and cyclohexanol (9.67%), and small amounts of alpha-pinene, o-methylisoprene and 1-methyl-1, 4-cyclohexadiene;

the main components of the clove oil are 3-allyl-6-methoxybenzene (79.25%), caryophyllene (15.92%) and a small amount of alpha-caryophyllene;

the main components of citral are geranial (96.0%) and a small amount of caryophyllene;

the main components of citronella oil are citronellal (34.1%), geraniol (19.53%), citronellol (13.2%) and small amounts of cyclohexanol, nerol E-acetate, 3, 7-dimethyl-6-octenol propionate, cyclohexylmethanol, etc.;

the main components of the wintergreen oil are benzoic acid (93.6 percent), a small amount of rosin oil, beta-pinene and the like;

the main components of rosemary oil are cycloheptyl ether (76.2%) and cyclohexanol (11.4), and a small amount of benzoin oil, cyclohexene and the like;

the main components of the peppermint oil are levomenthol (85.2 percent) and a small amount of levomenthol, isomenthone, pulegone, decyl acetate and the like;

the main components of thyme oil include thymol (68.5%), carvacrol (14.2%), etc.

In the research of predecessors, the effective components have certain bactericidal effect, but the traditional reagent makes the effective components difficult to be applied to human health protection, and the novel microemulsion is used in the patent, thereby being beneficial to human health.

Essential oil is a volatile aromatic substance extracted from flowers, leaves, stems, roots or fruits of plants by steam distillation, extrusion, cold immersion or solvent extraction, can control pathogenic microorganisms, and has recently attracted much attention at home and abroad. The research focuses on the comprehensive utilization of plant extract preparations, plant extracts are prepared into microemulsions, and the killing and inhibiting effects of the microemulsions on human skin pathogenic bacteria are detected.

The vegetable oil used in the invention is one or more of coconut oil, rape oil, wintergreen oil, thyme oil, peppermint oil, citronella oil, cinnamaldehyde, litsea cubeba oil, eucalyptus oil, lemon oil, rosemary oil and clove oil, has the function of killing pathogenic bacteria, has low environmental toxicity, and is clearly noted to avoid registration in the United states EPA 40CFR 152: 25B. The surfactant can make the vegetable oil uniformly disperse in water to form stable microemulsion, and the solvent can dilute. And the solvent used by the invention is a low VOC solvent, and is harmless to the ecological environment. The medicine is non-toxic to human body and has no pollution to environment, and belongs to an environment-friendly preparation.

The foregoing is only a preferred embodiment of this invention and it should be noted that those skilled in the art, having the benefit of the teachings of this invention, may effect numerous modifications thereto and changes may be made without departing from the scope of the invention as defined by the claims.

Claims

1. The application of a plant extract composition in preparing a medicament for controlling the activity of pathogenic bacteria related to skin diseases is characterized by comprising the following raw materials in percentage by weight: 10% of coconut oil, 40% of lactic acid, 0.1% of antioxidant and 100% of surfactant; the pathogenic bacteria are malassezia furfur, trichophyton rubrum and candida albicans.

2. Use according to claim 1, characterized in that: the antioxidant is one or more of tert-butyl hydroquinone (TBHQ), Butyl Hydroxy Anisol (BHA) and 2, 6-di-tert-butyl-p-cresol (BHT).