CN105505841A - Microorganism bacterium agent for treating industrial wastewater pollution - Google Patents
Microorganism bacterium agent for treating industrial wastewater pollution Download PDFInfo
- Publication number
- CN105505841A CN105505841A CN201610100190.3A CN201610100190A CN105505841A CN 105505841 A CN105505841 A CN 105505841A CN 201610100190 A CN201610100190 A CN 201610100190A CN 105505841 A CN105505841 A CN 105505841A
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- CN
- China
- Prior art keywords
- microbial inoculum
- industrial wastewater
- pseudomonas putida
- nitrococcus
- candidiasis
- Prior art date
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- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 19
- 239000010842 industrial wastewater Substances 0.000 title claims abstract description 12
- 241000894006 Bacteria Species 0.000 title claims abstract description 8
- 244000005700 microbiome Species 0.000 title abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 22
- 241000589597 Paracoccus denitrificans Species 0.000 claims abstract description 16
- 241000589776 Pseudomonas putida Species 0.000 claims abstract description 16
- 238000011282 treatment Methods 0.000 claims abstract description 15
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 13
- 241001147790 [Clostridium] papyrosolvens Species 0.000 claims abstract description 13
- 238000004065 wastewater treatment Methods 0.000 claims abstract description 7
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 4
- 241000605121 Nitrosomonas europaea Species 0.000 claims abstract description 4
- 241000222122 Candida albicans Species 0.000 claims description 12
- 206010007134 Candida infections Diseases 0.000 claims description 12
- 241001495402 Nitrococcus Species 0.000 claims description 12
- 201000003984 candidiasis Diseases 0.000 claims description 12
- 239000002068 microbial inoculum Substances 0.000 claims description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 9
- 239000002689 soil Substances 0.000 claims description 8
- 239000004615 ingredient Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 9
- 230000015556 catabolic process Effects 0.000 abstract description 5
- 238000006731 degradation reaction Methods 0.000 abstract description 5
- 230000008485 antagonism Effects 0.000 abstract description 3
- 150000001875 compounds Chemical class 0.000 abstract description 3
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 abstract description 3
- 150000001448 anilines Chemical class 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 20
- 239000000126 substance Substances 0.000 description 11
- 238000002360 preparation method Methods 0.000 description 10
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 8
- 230000008569 process Effects 0.000 description 8
- 241000233866 Fungi Species 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000002131 composite material Substances 0.000 description 5
- 239000004927 clay Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000002351 wastewater Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 229960000892 attapulgite Drugs 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 229910052625 palygorskite Inorganic materials 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 238000010170 biological method Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- SNRUBQQJIBEYMU-UHFFFAOYSA-N dodecane Chemical compound CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 239000011344 liquid material Substances 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- -1 olefines Chemical class 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- ISPYQTSUDJAMAB-UHFFFAOYSA-N 2-chlorophenol Chemical compound OC1=CC=CC=C1Cl ISPYQTSUDJAMAB-UHFFFAOYSA-N 0.000 description 1
- IQUPABOKLQSFBK-UHFFFAOYSA-N 2-nitrophenol Chemical compound OC1=CC=CC=C1[N+]([O-])=O IQUPABOKLQSFBK-UHFFFAOYSA-N 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 241000195940 Bryophyta Species 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- HZVVJJIYJKGMFL-UHFFFAOYSA-N almasilate Chemical compound O.[Mg+2].[Al+3].[Al+3].O[Si](O)=O.O[Si](O)=O HZVVJJIYJKGMFL-UHFFFAOYSA-N 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 150000004982 aromatic amines Chemical class 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 231100000704 bioconcentration Toxicity 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 238000007233 catalytic pyrolysis Methods 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 238000009388 chemical precipitation Methods 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 239000002734 clay mineral Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 150000001896 cresols Chemical class 0.000 description 1
- DIOQZVSQGTUSAI-NJFSPNSNSA-N decane Chemical group CCCCCCCCC[14CH3] DIOQZVSQGTUSAI-NJFSPNSNSA-N 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000000909 electrodialysis Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 239000008235 industrial water Substances 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004630 mental health Effects 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910052901 montmorillonite Inorganic materials 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- DIOQZVSQGTUSAI-UHFFFAOYSA-N n-butylhexane Natural products CCCCCCCCCC DIOQZVSQGTUSAI-UHFFFAOYSA-N 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- JMANVNJQNLATNU-UHFFFAOYSA-N oxalonitrile Chemical class N#CC#N JMANVNJQNLATNU-UHFFFAOYSA-N 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920000151 polyglycol Polymers 0.000 description 1
- 239000010695 polyglycol Substances 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000001223 reverse osmosis Methods 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 235000012222 talc Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Abstract
The invention relates to a microorganism bacterium agent for treating industrial wastewater pollution. The microorganism bacterium agent is prepared from pseudomonas putida, candida utilis, bacillus amyloliquefaciens, clostridium papyrosolvens, nitrosomonas europaea, paracoccus denitrificans and auxiliary materials. The microorganism bacterium agent has the advantages that various bacteria in mutualistic symbiosis are compatible reasonably without antagonism and are effective in degradation of phenyl amines degradation-resistant compounds when being thrown into a wastewater treatment system, thereby being suitable for industrial wastewater treatment and being capable of increasing treatment water quantity, improving treatment water quality, reducing operational cost and promoting up-to-standard discharge.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of microbiobacterial agent for the treatment of industrial wastewater pollution.
Background technology
Trade effluent complicated component, particularly a large amount of artificial-synthetic compound's entered environments, this kind of material mainly alkanes, olefines, alicyclic hydrocarbon type, arene, the organism of synthetic, especially macromole, difficult degradation, hazardous and noxious substances is as phenol, chlorophenol, cresols, nitrophenol etc., arene, cyanogen class, amine and ammonia nitrogen etc. are along with the waste water of plant emissions or solid waste entered environment, due to complicacy and the biological strange property of these these body structures of material, utilization can not be decomposed by the microorganisms at short notice, the microorganism of traditional method of wastewater treatment activated sludge culture can not be removed these pollutents effectively, these materials accumulate for a long time in the environment, the ecotope of depending on for existence to us causes very large pollution, very large harm is brought to the physical and mental health of the mankind.Quite a few industrial pollution enterprise of China would rather be punished also to be unwilling to invest and administer waste water, even if there have waste disposal plant to run to be also abnormal.Therefore, develop that a kind of construction investment is few, running cost is low, the sewage disposal technology of good treatment efficiency is extremely urgent.
Aniline is the most representative material of aromatic amine, it is a kind of colourless oil liquid with aromatic odour, be widely used in national defence, printing and dyeing, plastics, paint, agricultural chemicals and medicine industry etc., the objectionable impurities being simultaneously also serious environment pollution and being detrimental to health is that three of a kind of " carcinogenic, teratogenesis, mutagenesis " cause material.There is due to aniline the features such as extended residual, bioconcentration, carinogenicity, be classified as 129 kinds of pollutents of priority acccess control by U.S. EPA, be also put in " Chinese environmental priority pollutant Black List ", in industrial water drainage, require strict control.The traditional method of process aniline waste water mainly contains the methods such as physics, chemistry, biology.
Current process for treating industrial waste water mainly contains physical method reverse osmosis, distillation, soil irrigation; Chemical method has ion exchange method, ammonia aeration, chemical precipitation method, breakpoint chlorination, electrodialysis, electrochemical treatment, catalytic pyrolysis; Biological method has nitrated and algal culture, but physical method treatment effect is not good, than chemical method, biological method process waste water has the following advantages: 1) often kind of chemical article is all the product that specific aim is very strong, just likely lost efficacy when running into other chemical substances, and the removal of biotechnological formulation to pollutent has spectrum; 2) chemical products can temporarily eliminate some objectionable impurities and mask malodors, but can not stop the generation of objectionable impurities; 3), after using chemical products, have residual in water body, may secondary pollution be caused.Natural microbial contained by biotechnological formulation, not containing pathogenic bacterium and pathogenic agent, these microorganisms are under the katalysis of enzyme, with the organic nutrient substance in sewage for food, after sewage is purified, these microbes reduce gradually with the reduction of pollutent, until wither away; 4) nontoxic, non-corrosiveness, easy to use, substantially do not need interpolation equipment or engineering, save fund input.
Bioremediation administers the effective ways of industrial pollution water body.It is the effect utilizing microorganism, and compound is decomposed.Because bioremediation is more much lower than physics, chemical process cost, non-secondary pollution again, and microorganism has again stronger variability and adaptability, therefore it has become the Perfected process of process industrial pollution water body.
Summary of the invention
The object of this invention is to provide a kind of microbiobacterial agent for the treatment of industrial wastewater pollution.
Described trade effluent contains aniline.
Biotechnological formulation of the present invention, reasonable compatibility between each bacterial classification, symbiosis is coordinated, mutually not antagonism, and its preparation method is easy, easy to implement the method, and it is easy and simple to handle, is beneficial to production.
The present invention adopts following technical scheme to realize:
A kind of microbiobacterial agent for the treatment of industrial wastewater pollution, this microbial inoculum comprises following raw material: major ingredient and auxiliary material, and described major ingredient is prepared by pseudomonas putida, candidiasis, bacillus amyloliquefaciens, Clostridium papyrosolvens, nitrococcus, Paracoccus denitrificans, nitrifier.
Described auxiliary material can be solid adjuvant material and/or Auxiliary Liquid Material.Described solid adjuvant material is mineral material, biomaterial and/or macromolecular compound; Described mineral material can be at least one in clay, talcum, kaolin, montmorillonite, white carbon, zeolite, silica, turfy soil and diatomite; Described biomaterial is at least one in the ight soil of the stalk of all kinds of crop, loose shell, straw, Pericarppium arachidis hypogaeae, Semen Maydis powder, bean powder, starch, the peat composed of rotten mosses and animal; Described macromolecular compound is polyvinyl alcohol and/or polyglycol.Described Auxiliary Liquid Material can be organic solvent, vegetables oil, mineral oil or water; Described organic solvent is decane and/or dodecane.Described clay specifically can be attapulgite clay.The clay mineral of described attapulgite clay to be a kind of with attapulgite be main component, main chemical compositions is hydrous magnesium aluminosilicate, containing elements such as K, Na, Ca, Fe, Al, Mg, Mn, Ti, has unique dispersive ability and adsorptive power.
Through checking, final auxiliary material of selecting is made up of following raw material: diatomite, turfy soil.
Preferably, described auxiliary material is mixed according to weight ratio 4:1 by diatomite, turfy soil;
Described composite fungus agent and auxiliary material are mixed with biological restoration preparation according to the weight ratio of 2:5;
The preparation method of described composite fungus agent is as follows: pseudomonas putida, candidiasis, bacillus amyloliquefaciens, Clostridium papyrosolvens, nitrococcus, Paracoccus denitrificans, nitrifier being cultured to respectively concentration is 1 × 10
8the bacterium liquid of individual/ml, then mixes according to the volume ratio of 6:2:4:2:3:1:2, to obtain final product;
Preferably:
Described pseudomonas putida be pseudomonas putida (
pseudomonasputida) CCTCC203021(CN1513980 openly uses);
Described candidiasis be candidiasis (
candidautilis) ATCCNo.22023;
Described bacillus amyloliquefaciens be (
bacillusamyloliquefaciens) ATCC23843;
Described Clostridium papyrosolvens be (
clostridiumpapyrosolvens) ATCC700395;
Described nitrococcus be nitrococcus (
nitrosomonaseuropaea) CCTCCNo:M2010002(CN101955885);
Described Paracoccus denitrificans specifically can be Paracoccus denitrificans (Paracoccusdenitrificans) ATCC13543;
Described nitrifier is (Nitrobactersp.) CCTCC2010001 (CN101955885)
Bacterial classification of the present invention all can be bought and obtain from China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) and American Type Culture collection warehousing (ATCC), Chinese agriculture Microbiological Culture Collection administrative center (ACCC).
Those skilled in the art can select suitable substratum and enlarged culturing method according to general knowledge, make viable count reach 10
8individual/gram, and conveniently prepare the method preparation of microbial solid preparation.
Composite fungus agent of the present invention is by the various bacterial classification that can form dominant microflora, be mixed with high-efficiency microorganism preparation, be added in Waste Water Treatment by a certain amount of, accelerate the degraded of microbe, to improve the biological treatment efficiency of system, ensure system stable operation.It contains multiple microorganism Recalcitrant chemicals being had to excellent degradation capability, reasonable compatibility between each bacterial classification, and symbiosis is coordinated, mutual not antagonism, active high, biomass is large, and breeding is fast, add in Waste Water Treatment, there is good degradation effect to amino benzenes polluted water, be suitable for Industrial Waste Water Treatments, the process water yield and water quality treatment can be improved, reduce working cost, promote qualified discharge.
Embodiment
Embodiment 1
A kind of microbiobacterial agent for the treatment of industrial wastewater pollution, this microbial inoculum comprises following raw material: major ingredient and auxiliary material, and described major ingredient is prepared by pseudomonas putida, candidiasis, bacillus amyloliquefaciens, Clostridium papyrosolvens, nitrococcus, Paracoccus denitrificans, nitrifier.
Described auxiliary material is mixed according to weight ratio 4:1 by diatomite, turfy soil;
Described composite fungus agent and auxiliary material are mixed with biological restoration preparation according to the weight ratio of 2:5;
The preparation method of described composite fungus agent is as follows: pseudomonas putida, candidiasis, bacillus amyloliquefaciens, Clostridium papyrosolvens, nitrococcus, Paracoccus denitrificans, nitrifier being cultured to respectively concentration is 1 × 10
8the bacterium liquid of individual/ml, then mixes according to the volume ratio of 6:2:4:2:3:1:2, to obtain final product;
Preferably:
Described pseudomonas putida be pseudomonas putida (
pseudomonasputida) CCTCC203021(CN1513980 openly uses);
Described candidiasis be candidiasis (
candidautilis) ATCCNo.22023;
Described bacillus amyloliquefaciens be (
bacillusamyloliquefaciens) ATCC23843;
Described Clostridium papyrosolvens be (
clostridiumpapyrosolvens) ATCC700395;
Described nitrococcus be nitrococcus (
nitrosomonaseuropaea) CCTCCNo:M2010002(CN101955885);
Described Paracoccus denitrificans specifically can be Paracoccus denitrificans (Paracoccusdenitrificans) ATCC13543;
Described nitrifier is (Nitrobactersp.) CCTCC2010001 (CN101955885)
Bacterial classification of the present invention all can be bought and obtain from China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) and American Type Culture collection warehousing (ATCC), Chinese agriculture Microbiological Culture Collection administrative center (ACCC).
Those skilled in the art can select suitable substratum and enlarged culturing method according to general knowledge, make viable count reach 10
8individual/gram, and conveniently prepare the method preparation of microbial solid preparation.
Embodiment 2
Adopt microbiobacterial agent process chemical plant trade effluent prepared by embodiment 1, treatment process is the filter of A/O+ precipitation+aeration and biological, A/O pond adds the obtained microbiobacterial agent 20 grams of embodiment 1 by every cubic metre of biochemistry pool volume at every turn, adds 1 every day, adds 10 days continuously; BAF adds the obtained microbiobacterial agent 20 grams of embodiment 1 by every cubic meter filler, adds 1 every day, adds 10 days continuously; After one month, treatment effect is as following table 1:
Table 1
Although above done detailed explanation with general explanation and embodiment to this case, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, amendment done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.
Claims (8)
1. for the treatment of the microbiobacterial agent of industrial wastewater pollution, it comprises following raw material: major ingredient and auxiliary material.
2. microbial inoculum according to claim 1, is characterized in that, described major ingredient and auxiliary material mix according to the weight ratio of 2:5.
3. the microbial inoculum according to claim 1-2, is characterized in that, described major ingredient is prepared by pseudomonas putida, candidiasis, bacillus amyloliquefaciens, Clostridium papyrosolvens, nitrococcus, Paracoccus denitrificans, nitrifier and obtained.
4. the microbial inoculum according to claim 1-3, is characterized in that, it is 1 × 10 that described microbial inoculum is cultured to concentration respectively by pseudomonas putida, candidiasis, bacillus amyloliquefaciens, Clostridium papyrosolvens, nitrococcus, Paracoccus denitrificans, nitrifier
8the bacterium liquid of individual/ml, is then mixed with according to the volume ratio of 6:2:4:2:3:1:2 and obtains.
5. the microbial inoculum according to claim 1-4, is characterized in that, described auxiliary material is diatomite and turfy soil.
6. the microbial inoculum according to claim 1-5, is characterized in that, described auxiliary material is mixed according to weight ratio 4:1 by diatomite and turfy soil.
7. the microbial inoculum according to claim 3-6, is characterized in that,
Described pseudomonas putida be pseudomonas putida (
pseudomonasputida) CCTCCNo.203021;
Described candidiasis be candidiasis (
candidautilis) ATCC22023;
Described bacillus amyloliquefaciens be (
bacillusamyloliquefaciens) ATCC23843;
Described Clostridium papyrosolvens be (
clostridiumpapyrosolvens) ATCC700395;
Described nitrococcus be nitrococcus (
nitrosomonaseuropaea) CCTCCNo:M2010002;
Described Paracoccus denitrificans is Paracoccus denitrificans (Paracoccusdenitrificans) ATCC13543;
Described nitrifier be (
nitrobactersp.) CCTCCNo.2010001.
8. microbial inoculum described in claim 1-7 is used for the application of Industrial Wastewater Treatment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610100190.3A CN105505841B (en) | 2016-02-24 | 2016-02-24 | For handling the microbial bacterial agent of industrial wastewater pollution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610100190.3A CN105505841B (en) | 2016-02-24 | 2016-02-24 | For handling the microbial bacterial agent of industrial wastewater pollution |
Publications (2)
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| CN105800883A (en) * | 2016-05-18 | 2016-07-27 | 杭州富阳高博信息技术服务有限公司 | Microbial preparation for urban green land sewage treatment and using method thereof |
| CN106011038A (en) * | 2016-08-04 | 2016-10-12 | 广东紫方环保技术有限公司 | Compound microbial agent for treating polluted water and preparation method thereof |
| CN106082549A (en) * | 2016-08-03 | 2016-11-09 | 杭州富阳佳畅机械有限公司 | The biophysics of electroplating wastewater processes technique |
| CN106082452A (en) * | 2016-08-03 | 2016-11-09 | 杭州富阳佳畅机械有限公司 | A kind of preparation of advanced treatment of electroplating waste |
| CN106116042A (en) * | 2016-08-03 | 2016-11-16 | 杭州富阳佳畅机械有限公司 | A kind of method synchronizing to remove the advanced treating electroplating sewerage of cyanogen, chromium |
| CN106348558A (en) * | 2016-09-30 | 2017-01-25 | 洛阳茂生生物技术有限公司 | Method for treating sludge of sewage plant by adopting microbial agent |
| CN106517535A (en) * | 2016-09-30 | 2017-03-22 | 洛阳茂生生物技术有限公司 | Method for treating sewage from sewage plant |
| CN105923900B (en) * | 2016-05-18 | 2018-08-24 | 江西福乐园林有限责任公司 | Sewage water treatment method in a kind of civic landscape planning |
| CN108641985A (en) * | 2018-05-21 | 2018-10-12 | 万创青绿（北京）环境科技有限公司 | A kind of complex microorganism cleanser and preparation method thereof for river sewage purification |
| CN109266590A (en) * | 2018-11-27 | 2019-01-25 | 合肥市东方美捷分子材料技术有限公司 | A kind of denitrification compound bacteria agent and its preparation method and application |
| CN110819560A (en) * | 2019-11-07 | 2020-02-21 | 北京师范大学 | Preparation and application method of composite microbial inoculum for efficiently treating chemical wastewater |
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| CN105753272A (en) * | 2016-05-14 | 2016-07-13 | 王浩丞 | Sewage treatment method for garden planning |
| CN105753272B (en) * | 2016-05-14 | 2018-11-16 | 王浩丞 | Sewage water treatment method in a kind of Garden Planning |
| CN105800883B (en) * | 2016-05-18 | 2018-07-17 | 佛山市碧沃丰生物科技股份有限公司 | A kind of microorganism formulation and its application method for urban green space sewage disposal |
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| CN106082452B (en) * | 2016-08-03 | 2019-05-03 | 杭州富阳佳畅机械有限公司 | A kind of preparation of advanced treatment of electroplating waste |
| CN106082549A (en) * | 2016-08-03 | 2016-11-09 | 杭州富阳佳畅机械有限公司 | The biophysics of electroplating wastewater processes technique |
| CN106116042B (en) * | 2016-08-03 | 2019-04-30 | 杭州富阳佳畅机械有限公司 | It is a kind of it is synchronous removal cyanogen, chromium advanced treating electroplating sewerage method |
| CN106116042A (en) * | 2016-08-03 | 2016-11-16 | 杭州富阳佳畅机械有限公司 | A kind of method synchronizing to remove the advanced treating electroplating sewerage of cyanogen, chromium |
| CN106082452A (en) * | 2016-08-03 | 2016-11-09 | 杭州富阳佳畅机械有限公司 | A kind of preparation of advanced treatment of electroplating waste |
| CN106082549B (en) * | 2016-08-03 | 2019-04-19 | 杭州富阳佳畅机械有限公司 | The biophysics treatment process of electroplating wastewater |
| CN106011038A (en) * | 2016-08-04 | 2016-10-12 | 广东紫方环保技术有限公司 | Compound microbial agent for treating polluted water and preparation method thereof |
| CN106517535A (en) * | 2016-09-30 | 2017-03-22 | 洛阳茂生生物技术有限公司 | Method for treating sewage from sewage plant |
| CN106348558A (en) * | 2016-09-30 | 2017-01-25 | 洛阳茂生生物技术有限公司 | Method for treating sludge of sewage plant by adopting microbial agent |
| CN108641985A (en) * | 2018-05-21 | 2018-10-12 | 万创青绿（北京）环境科技有限公司 | A kind of complex microorganism cleanser and preparation method thereof for river sewage purification |
| CN108641985B (en) * | 2018-05-21 | 2021-04-09 | 万创青绿（北京）环境科技有限公司 | Composite microbial purifying agent for purifying river sewage and preparation method thereof |
| CN109266590A (en) * | 2018-11-27 | 2019-01-25 | 合肥市东方美捷分子材料技术有限公司 | A kind of denitrification compound bacteria agent and its preparation method and application |
| CN110819560A (en) * | 2019-11-07 | 2020-02-21 | 北京师范大学 | Preparation and application method of composite microbial inoculum for efficiently treating chemical wastewater |
| CN112725242A (en) * | 2021-02-10 | 2021-04-30 | 杭州楠大环保科技有限公司 | Special microbial inoculum for concentrated sewage treatment of reinforced activated sludge and preparation method thereof |
| CN112725242B (en) * | 2021-02-10 | 2023-12-05 | 杭州楠大环保科技有限公司 | Special microbial inoculum for strengthening sewage centralized treatment of activated sludge and preparation method thereof |
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