CN112725242B - Special microbial inoculum for strengthening sewage centralized treatment of activated sludge and preparation method thereof - Google Patents
Special microbial inoculum for strengthening sewage centralized treatment of activated sludge and preparation method thereof Download PDFInfo
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- CN112725242B CN112725242B CN202110183154.9A CN202110183154A CN112725242B CN 112725242 B CN112725242 B CN 112725242B CN 202110183154 A CN202110183154 A CN 202110183154A CN 112725242 B CN112725242 B CN 112725242B
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- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 80
- 239000010865 sewage Substances 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title claims abstract description 32
- 238000005728 strengthening Methods 0.000 title claims abstract description 27
- 239000000463 material Substances 0.000 claims abstract description 62
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- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 claims abstract description 25
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/06—Sludge reduction, e.g. by lysis
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Water Supply & Treatment (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention discloses a special microbial agent for strengthening sewage centralized treatment of activated sludge and a preparation method thereof, and relates to the technical field of microbial agents. The microbial inoculum comprises: the main material is composite bacteria including paracoccus denitrificans, clostridium paperlyticum, trichoderma harzianum, nitrifying bacteria, candida and bacillus amyloliquefaciens; auxiliary materials including potassium salt of full allyl oxo cucurbit [6] uril sulfate, nano-level calcium aluminum garnet powder and peptone; the weight ratio of the main materials to the auxiliary materials is 1:2 to 4. The microbial inoculum prepared by the invention has excellent strengthening performance, can obviously reduce the contents of substances such as polysaccharide, protein and the like in a sludge system, and can degrade and utilize organic matters such as EPS and the like to a greater extent; and effectively enhance the sludge reduction effect and improve the wastewater treatment effect.
Description
Technical Field
The invention belongs to the technical field of microbial agents, and particularly relates to a special microbial agent for strengthening sewage centralized treatment of activated sludge and a preparation method thereof.
Background
The technology for treating sewage and wastewater by the activated sludge process has the characteristics of good sewage treatment effect, mature technology, multiple process types and the like, and is widely applied to countries of the world. When the activated sludge process is used for treating wastewater, if the activated sludge is directly put into a reactor for operation, microorganisms cannot adapt to water environment, so that the microorganisms die, and a large amount of activated sludge is inactivated. Thus, prior to formal operation, domestication is required so that microorganisms therein grow. However, the time taken for domestication is long, and the running cost is increased. The addition of effective microorganisms greatly improves the microbial load and activity of the system, thereby shortening the domestication and running time of the system and improving the treatment efficiency. At present, the bio-enhancement technology is widely used, and a great deal of research on the enhancement technology is studied and confirmed, but the bio-enhancement technology cannot be widely used for various reasons. The key point of the technology is the extraction and culture of effective microorganisms.
And the activated sludge process for treating sewage has a major disadvantage of producing a large amount of surplus sludge. The residual sludge contains heavy metals, pathogenic bacteria and other toxic and harmful substances, and if the residual sludge is improperly treated, secondary pollution is caused to the environment, and the residual sludge threatens the health of human beings. In addition, the treatment cost of the sludge is high, and the treatment cost of the sludge accounts for about 30-50% of the total investment of the sewage treatment plant according to statistics, and the running cost accounts for 50-60% of the total running cost, so that the sludge outlet of the sewage treatment plant is a very troublesome problem. There are demands for reduction, harmlessness and recycling of excess sludge treatment at home and abroad, and a great number of sludge treatment and treatment methods, such as sludge concentration, dehydration, sludge stabilization and other treatment methods, and incineration, landfill, composting, comprehensive utilization of resources and other treatment methods, have been studied. Although there are many treatment methods for sludge, there are various advantages and disadvantages of each method in view of the treatment effect, the economical efficiency and the safety of the treatment method, and no satisfactory method has been found so far.
Disclosure of Invention
The invention aims to provide a special microbial inoculum for strengthening the centralized sewage treatment of activated sludge and a preparation method thereof, wherein the microbial inoculum has excellent strengthening performance, reduces the contents of substances such as polysaccharide, protein and the like in a sludge system, enhances the sludge reduction effect and improves the wastewater treatment effect.
The technical scheme adopted by the invention for achieving the purpose is as follows:
the invention discloses an application of full allyl oxo cucurbit [6] uril potassium sulfate and nano-scale calcium aluminum garnet powder in improving the action of microbial inoculum to strengthen sludge. The synergistic effect of the full allyl oxo-cucurbit [6] uril potassium sulfate and the nano-level calcium aluminum garnet powder can be used for conditioning the concentrated sludge, and the reason is probably that the flocculation and dispersion of the particle molecules are in an equilibrium state by penetrating sludge flocs and dispersing the particle molecules, so that the action range is enlarged, the digestion level is effectively improved, various biochemical indexes of a sludge system are enhanced, the polysaccharide, protein content and EPS total amount are reduced, and organic matters such as EPS and the like can be degraded to a greater extent and utilized.
A microbial agent comprising:
the main material is composite bacteria including paracoccus denitrificans, clostridium paperlyticum, trichoderma harzianum, nitrifying bacteria, candida and bacillus amyloliquefaciens;
auxiliary materials including potassium salt of full allyl oxo cucurbit [6] uril sulfate, nano-level calcium aluminum garnet powder and peptone;
the weight ratio of the main materials to the auxiliary materials is 1:2 to 4. The microorganisms in the composite bacteria are mutually coordinated to achieve the optimal decontamination effect, the treatment method is simple and convenient, the peculiar smell brought by sewage is greatly reduced in the treatment process, flies and mosquitoes are avoided, and the environmental pollution is reduced. The synergistic effect of the full allyl oxo-cucurbit [6] uril potassium sulfate and the nano-level calcium aluminum garnet powder in the auxiliary materials is used for conditioning the concentrated sludge, so that the aggregation and dispersion among the particle molecules are in an equilibrium state by penetrating sludge flocs and dispersing the particle molecules, the action range is enlarged, the digestion level is effectively improved, various biochemical indexes of a sludge system are enhanced, the polysaccharide, protein content and EPS total amount are reduced, and organic matters such as EPS and the like can be degraded to a greater extent and utilized. After the microbial inoculum is added, the action effect of the reinforced activated sludge can be obviously improved, and the effect of removing COD, nitrogen and phosphorus in sewage can be enhanced; in addition, the sludge reduction effect can be remarkably enhanced.
Preferably, the composite bacteria comprise 9-12 parts by weight of paracoccus denitrificans, 8-12 parts by weight of clostridium paperlyticum, 6-9 parts by weight of trichoderma harzianum, 5-8 parts by weight of nitrifying bacteria, 4-6 parts by weight of candida and 3-6 parts by weight of bacillus amyloliquefaciens.
Preferably, the mass ratio of the full allyl oxo cucurbit [6] uril potassium sulfate, the nanoscale calcium aluminum garnet powder and the peptone in the auxiliary materials is 1:0.9 to 1.2:0.8 to 1.
Preferably, the auxiliary material component is sclareol or sclareol and sclareol. The sclareol or the liquid auxiliary material consisting of the sclareol and the sclareol is adopted to replace the solid auxiliary material, the prepared microbial inoculum still has excellent strengthening effect, can effectively strengthen various biochemical indexes of a sludge system, reduces the content of polysaccharide, protein and the total amount of EPS, and particularly can obviously strengthen the degradation of protein due to the existence of sclareol. And the effect of removing COD and nitrogen in the sewage can be effectively enhanced, and the sludge reduction effect is enhanced.
It is a further object of the present invention to provide the use of the microbial inoculum described above for enhancing activated sludge or sewage treatment.
The preparation method of the microbial inoculum comprises the following steps:
preparing a composite microbial bacterial liquid, namely respectively carrying out primary slant culture and secondary liquid culture on paracoccus denitrificans, clostridium paperlyticum, trichoderma harzianum, nitrifying bacteria, candida and bacillus amyloliquefaciens to obtain a microbial fermentation liquid, and then mixing to obtain the composite microbial bacterial liquid;
the auxiliary material is prepared by mixing full allyl oxo cucurbit [6] uril potassium sulfate, nano-level calcium aluminum garnet powder and peptone;
and (3) preparing a microbial agent, namely uniformly mixing the composite microbial liquid with auxiliary materials to obtain the microbial agent.
Preferably, the compound microbial liquid prepared by the preparation of the compound microbial liquid is added with the tanguticin with the mass concentration of 0.08-0.1%. Adding the honey orange flavin into the obtained compound microorganism bacterial liquid, and then compounding with auxiliary materials to prepare the bacterial agent, wherein the honey orange flavin can act on bacterial strains, promote the secretion of enzymes such as protease, amylase and the like, promote the enzyme activity, and further enhance the action effect of the bacterial agent reinforced activated sludge.
Preferably, in the preparation of the composite microbial liquid, each of the bacteria is cultured until the number of the bacteria is 2 to 5×10 8 And each mL.
Preferably, a person skilled in the art can select an appropriate medium and an enlarged culture method according to common knowledge.
The invention also discloses the application of the microbial inoculum prepared by the preparation method in strengthening the sewage centralized treatment of activated sludge.
Preferably, the use amount of the microbial inoculum is 1-5 wt%o of the total amount of the treated sewage.
Compared with the prior art, the invention has the following beneficial effects:
the microorganisms in the composite bacteria of the main material of the microbial inoculum prepared by the invention are mutually coordinated to achieve the optimal decontamination effect; the auxiliary materials are fully allyl oxo-cucurbit [6] uril potassium sulfate and nano calcium aluminum garnet powder cooperate to condition the concentrated sludge, strengthen various biochemical indexes of a sludge system, reduce the content of polysaccharide, protein and the total amount of EPS, and degrade and utilize organic matters such as EPS to a greater extent. And the honey orange is added into the obtained compound microbial bacterial liquid, so that secretion of bacterial enzymes can be effectively promoted, enzyme activity can be improved, and further the action effect of the microbial inoculum reinforced activated sludge can be enhanced; after the microbial inoculum is added, the action effect of the reinforced activated sludge can be obviously improved, and the effect of removing COD, nitrogen and phosphorus in sewage can be enhanced; and effectively enhances the sludge reduction effect. Meanwhile, the liquid auxiliary materials composed of the sclareol and the sclareol are adopted to replace the solid auxiliary materials, the prepared microbial inoculum still has excellent strengthening effect, and compared with the prior art, the strengthening effect of the microbial inoculum is improved to a certain extent, and especially the degradation of proteins can be obviously enhanced due to the existence of the sclareol.
Therefore, the invention provides the special microbial inoculum for strengthening the centralized sewage treatment of the activated sludge and the preparation method thereof, and the microbial inoculum has excellent strengthening performance, reduces the contents of substances such as polysaccharide, protein and the like in a sludge system, enhances the sludge reduction effect and improves the wastewater treatment effect.
Drawings
FIG. 1 shows the results of the enzyme content test in test example 1 of the present invention.
Detailed Description
The technical scheme of the invention is further described in detail below with reference to the specific embodiments and the attached drawings:
the paracoccus denitrificans used in the examples of the present invention was (Paracoccus denitrificans) ATCC 13543; clostridium paperlyticum (Clostridium papyrosolvens) ATCC 700395; trichoderma harzianum (Trichoderma harzianum) CGMCC No.5547; the nitrifying bacteria is (Nitrobacter sp.) CCTCC No.2010001; the Candida is (Candida utilis) ATCC 22023; the Bacillus amyloliquefaciens used was (Bacillus amyloliquefaciens) ATCC23843.
Example 1:
a special microbial inoculum for strengthening sewage centralized treatment of activated sludge comprises the following components:
the main materials comprise 11 parts of paracoccus denitrificans, 10 parts of clostridium paperlyticum, 8 parts of trichoderma harzianum, 7 parts of nitrifying bacteria, 5 parts of candida and 5 parts of bacillus amyloliquefaciens;
auxiliary materials including full allyl oxo cucurbit [6] uril potassium sulfate, nano-level calcium aluminum garnet powder and peptone, wherein the mass ratio is 1:1:1.
the weight ratio of the main materials to the auxiliary materials is 1:3.6.
the preparation method of the microbial inoculum comprises the following steps:
preparing compound microorganism bacterial liquid, respectively performing primary slant culture and secondary liquid culture on Paracoccus denitrificans, clostridium paperlyticum, trichoderma harzianum, nitrifying bacteria, candida and Bacillus amyloliquefaciens, and culturing until the bacterial count is 4×10 8 individual/mL;
the auxiliary material is prepared by mixing full allyl oxo cucurbit [6] uril potassium sulfate, nano-level calcium aluminum garnet powder and peptone;
and (3) preparing a microbial agent, namely uniformly mixing the composite microbial liquid with auxiliary materials to obtain the microbial agent.
Wherein, in the preparation process of the compound microorganism bacterial liquid, the culture medium for primary slant culture is PDA solid culture medium; in the secondary liquid culture process, the LB liquid culture medium for culturing the paracoccus denitrificans is subjected to shaking culture at 25-30 ℃; culturing Clostridium paperlyticum by shaking culture in DMEM liquid culture medium at 30-35 deg.C; liquid PDA culture medium (agar is removed) for Trichoderma harzianum culture, and shake culture is carried out at 23-25 ℃; LB liquid culture medium for nitrifying bacteria culture is subjected to shaking culture at 28-30 ℃; liquid PDA culture medium (agar is removed) for culturing candida, and shaking culture is carried out at 25-28 ℃; beef extract liquid culture medium for culturing bacillus amyloliquefaciens is subjected to shaking culture at 35-37 ℃.
Example 2:
a special microbial inoculum for strengthening sewage centralized treatment of activated sludge comprises the following components:
the main materials comprise 9 parts of paracoccus denitrificans, 10 parts of clostridium paperlyticum, 6 parts of trichoderma harzianum, 5 parts of nitrifying bacteria, 6 parts of candida and 4 parts of bacillus amyloliquefaciens;
auxiliary materials including full allyl oxo cucurbit [6] uril potassium sulfate, nano-level calcium aluminum garnet powder and peptone, wherein the mass ratio is 1:0.9:0.8.
the weight ratio of the main materials to the auxiliary materials is 1:2.
the preparation method of the microbial inoculum is different from that of the embodiment 1 in that: each strain is cultivated until the bacterial count is 3 multiplied by 10 8 And each mL.
Example 3:
a special microbial inoculum for strengthening sewage centralized treatment of activated sludge comprises the following components:
the main materials comprise 10 parts of paracoccus denitrificans, 10 parts of clostridium paperlyticum, 7 parts of trichoderma harzianum, 7 parts of nitrifying bacteria, 4 parts of candida and 5 parts of bacillus amyloliquefaciens;
auxiliary materials including full allyl oxo cucurbit [6] uril potassium sulfate, nano-level calcium aluminum garnet powder and peptone, wherein the mass ratio is 1:1.1:0.9.
the weight ratio of the main materials to the auxiliary materials is 1:2.8.
the preparation method of the microbial inoculum is different from that of the embodiment 1 in that: the volume ratio of the microbial fermentation liquid of the paracoccus denitrificans, the clostridium paperlyticum, the trichoderma harzianum, the nitrifying bacteria, the candida and the bacillus amyloliquefaciens is 4:3.2:2:1.5:1.5:0.8.
example 4:
a special microbial inoculum for strengthening sewage centralized treatment of activated sludge comprises the following components:
the main materials comprise 11 parts of paracoccus denitrificans, 9 parts of clostridium paperlyticum, 8 parts of trichoderma harzianum, 6 parts of nitrifying bacteria, 4 parts of candida and 3 parts of bacillus amyloliquefaciens;
auxiliary materials including full allyl oxo cucurbit [6] uril potassium sulfate, nano-level calcium aluminum garnet powder and peptone, wherein the mass ratio is 1:1.2:1.
the weight ratio of the main materials to the auxiliary materials is 1:3.
the preparation method of the microbial inoculum is different from that of the embodiment 1 in that: each strain is cultivated until the bacterial count is 3.6X10 8 And each mL.
Example 5:
a special microbial inoculum for strengthening sewage centralized treatment of activated sludge comprises the following components:
the main materials comprise 9 parts of paracoccus denitrificans, 12 parts of clostridium paperlyticum, 6 parts of trichoderma harzianum, 7 parts of nitrifying bacteria, 5 parts of candida and 4 parts of bacillus amyloliquefaciens;
auxiliary materials including full allyl oxo cucurbit [6] uril potassium sulfate, nano-level calcium aluminum garnet powder and peptone, wherein the mass ratio is 1:1:0.9.
the weight ratio of the main materials to the auxiliary materials is 1:4.
the preparation method of the microbial inoculum is different from that of the embodiment 1 in that: each strain is cultivated until the bacterial count is 2.8X10 8 And each mL.
Example 6:
a special microbial inoculum for strengthening sewage centralized treatment of activated sludge comprises the following components:
the main materials comprise 12 parts of paracoccus denitrificans, 10 parts of clostridium paperlyticum, 7 parts of trichoderma harzianum, 8 parts of nitrifying bacteria, 5 parts of candida and 3 parts of bacillus amyloliquefaciens;
auxiliary materials including full allyl oxo cucurbit [6] uril potassium sulfate, nano-level calcium aluminum garnet powder and peptone, wherein the mass ratio is 1:0.9:1.
the weight ratio of the main materials to the auxiliary materials is 1:3.5.
the preparation method of the microbial inoculum is different from that of the embodiment 1 in that: each strain is cultivated until the bacterial count is 4.5 multiplied by 10 8 And each mL.
Example 7:
the special microbial inoculum for the concentrated sewage treatment of the reinforced activated sludge is different from the microbial inoculum of the embodiment 1 in that:
the auxiliary material is sclareum oil.
The preparation method of the microbial inoculum is the same as that of the example 1.
Example 8:
the special microbial inoculum for the concentrated sewage treatment of the reinforced activated sludge is different from the microbial inoculum of the embodiment 1 in that:
the auxiliary materials are sclareol and sclareol oil, and the solid-liquid ratio of the sclareol to sclareol is 1g:10mL.
The preparation method of the microbial inoculum is the same as that of the example 1.
Example 9:
a special microbial inoculum for intensive sewage treatment of activated sludge was the same as in example 1.
The preparation method of the microbial inoculum is different from that of the embodiment 1 in that:
the prepared compound microbial liquid is added with the tanguticin with the mass concentration of 0.09 percent.
Example 10:
a special microbial inoculum for intensive sewage treatment of activated sludge was the same as in example 7.
The preparation method of the microbial inoculum is different from that of the example 7 in that:
the prepared compound microbial liquid is added with the tanguticin with the mass concentration of 0.09 percent.
Example 11:
a special microbial inoculum for intensive sewage treatment of activated sludge was the same as in example 8.
The preparation method of the microbial inoculum is different from that of the example 8 in that:
the prepared compound microbial liquid is added with the tanguticin with the mass concentration of 0.09 percent.
Comparative example 1:
the special microbial inoculum for the concentrated sewage treatment of the reinforced activated sludge is different from the microbial inoculum of the embodiment 1 in that:
the auxiliary material does not contain potassium salt of full allyl oxo-cucurbit [6] uril sulfate.
The preparation method of the microbial inoculum is the same as that of the example 1.
Comparative example 2:
the special microbial inoculum for the concentrated sewage treatment of the reinforced activated sludge is different from the microbial inoculum of the embodiment 1 in that:
the auxiliary materials do not contain nano-scale calcium aluminum garnet powder.
The preparation method of the microbial inoculum is the same as that of the example 1.
Comparative example 3:
the special microbial inoculum for the concentrated sewage treatment of the reinforced activated sludge is different from the microbial inoculum of the embodiment 1 in that:
beta-cyclodextrin is added into the auxiliary material to replace potassium salt of full allyl oxo-cucurbit [6] uril sulfate.
The preparation method of the microbial inoculum is the same as that of the example 1.
Comparative example 4:
the special microbial inoculum for the concentrated sewage treatment of the reinforced activated sludge is different from that of comparative example 3 in that:
the auxiliary materials do not contain nano-scale calcium aluminum garnet powder.
The preparation method of the microbial inoculum is the same as that of comparative example 3.
Test example 1:
enzyme assay
Preparation of the liquid: adding 0.09% of tangerines into the compound microorganism bacterial liquid, carrying out mixed culture for 12 hours, centrifuging, and taking supernatant for later use.
Protease assay: taking 1mL of the preparation solution, adding 1mL of casein (water bath at 60 ℃ for 5 min), adding 2mL of trichloroacetic acid, shaking uniformly, standing for 10min, filtering to reach a constant volume of 10mL, and measuring absorbance at 275nm wavelength by using a spectrophotometer.
Amylase assay: sterilizing 2mL of the preparation solution, adding starch, oscillating in water bath for 90min, centrifuging, developing the supernatant with DNS, cooling, fixing the volume, and measuring absorbance at 510 nm.
The above test was performed on the composite microorganism fermentation broths prepared in example 1 and example 9, and the results are shown in fig. 1. As can be seen from the figure, the protease and amylase contents of the composite microbial fermentation broth prepared in example 9 are 110.42 mug/mL.h and 86.49 mug/mL.h, which are obviously higher than those of example 1, and the addition of the exoenzyme of the strain can be promoted.
Test example 2:
diluting raw sludge with distilled water until the sludge concentration MLSS is about 3500mg/L, adding the microbial inoculum with the addition amount of 2 wt%o of the treated sewage, not adding the control group, carrying out shake culture on the sludge mixed solution of all the microbial inoculum and the control group at 28 ℃ for 7d at 80-120 r/min, and measuring the content of polysaccharide and protein in MLSS, MLVSS, EPS.
EPS assay
Preparing an appropriate amount of sludge mixed solution, centrifuging at 5000r/min for 5min, filtering with a 0.45 μm filter membrane, preserving, supplementing the stock solution with deionized water to original volume, mixing, heating at 60deg.C for 20min, centrifuging at 12000r/min for 10min, filtering with a 0.45 μm filter membrane, and mixing with the above step.
Determination of polysaccharide: taking 2mL of extracting solution, adding 4mL of anthrone reagent (for preparation at present), shaking uniformly, and measuring the absorbance value at 610 nm;
measurement of protein: the absorbance was measured at 595nm after shaking the extract with Coomassie brilliant blue reagent.
The bacterial agents prepared in comparative examples 1 to 4 and examples 1 to 11 were subjected to the above test, and the results are shown in Table 1.
Table 1 test results
From the analysis in Table 1, the polysaccharide content of the microbial inoculum prepared in the embodiment 1 is lower than that of the control group and the comparative examples 1-4, which shows that the degradation of polysaccharide substances is enhanced after the microbial inoculum is added, and the synergistic effect of the microbial inoculum and the microbial inoculum can effectively improve the enhancement effect due to the existence of the full allyl oxo cucurbit [6] uril potassium sulfate and the nano-scale calcium aluminum garnet powder; the protein content is obviously lower than that of a control group and slightly lower than that of comparative examples 1-4, which shows that the microbial inoculum is favorable for degrading protein after being added, and the existence of the full allyl oxo-cucurbit [6] uril potassium sulfate and the nano-level calcium aluminum garnet powder plays a synergistic effect, but has limited degradation; the total amount of EPS was significantly lower than that of the control and comparative examples 1 to 4; the MLVSS/MLSS value of example 1 is reduced by 15.2% compared with the control group, and is respectively reduced by 12.2%, 8.0%, 11.0% and 5.6% compared with comparative examples 1-4, which shows that the addition of the microbial inoculum can promote the decomposition of organic matters in the system, and the existence of the full allyl oxo-cucurbit [6] uril potassium sulfate and the nano-scale calcium aluminum garnet powder plays a synergistic effect. The effect of example 8 is equivalent to that of example 1 and slightly better than that of example 7, and shows that the sclareol has good strengthening effect by taking sclareol as a liquid auxiliary material, and the existence of sclareol has synergistic strengthening effect, and particularly has obvious effect of improving the degradation effect of protein. The effects of example 9 are better than those of example 1, example 10 and example 7 and example 11 than example 8, and the fact that the biological activity of the microbial inoculum can be effectively improved by adding the pummelo peel, so that the effect is enhanced.
Test example 3:
simulated sewage treatment test
Experimental device
Two reactors (one dosed as dosing group and the other without as control group), each reactor size: the effective volume of 0.5X0.5X0.9 m, 0.5X0.5X0.7=175L, wherein one surface of the reactor is respectively provided with holes at positions which are 30mm, 100mm, 300mm and 600mm away from the bottom of the tank for mud discharge and drainage, the aperture phi=25 mm, the reactor is made of a 7mm thick PVC plate, one surface of the reactor adopts a transparent plate and is marked with scales, and the scale marks are respectively 100mm, 200mm, 300mm, 400mm, 500mm, 600mm, 700mm and 800mm away from the bottom of the reactor, so that the water inlet and outlet height and the sludge layer height can be conveniently observed. 2 small fans, 1 reactor per reactor, model: ACQ-005, 220-240V, 50Hz, power 85W, air volume 60L/min.
Sludge inoculation
The inoculated sludge is taken from fresh surplus activated sludge discharged from a sewage treatment plant. In order to facilitate the comparison test, the sludge concentration after inoculation of the dosing group and the control group is as much as possible, and the addition amount of the microbial inoculum in the dosing group is 2 wt%o of the sewage treatment amount. And (5) inoculating the sludge, domesticating and culturing for 10 days, and then starting to add the microbial inoculum into the dosing group. Specific indexes of the sludge are shown in Table 2:
table 2 Main sludge index used in experiments
| Index (I) | pH | MLSS(mg/L) | MLVSS(mg/L) | SV 30 |
| Numerical value | 6.0～6.8 | 4000～6000 | 3600～4500 | 20～61 |
The main water quality indexes of the self-matching simulated sewage are shown in table 3:
TABLE 3 quality of incoming water
| Index (I) | PH | COD(mg/L) | NH 4 + -N(mg/L) | TN(mg/L) | TP(mg/L) |
| Numerical value | 6.8 | 490 | 35 | 46 | 5.8 |
The discharge water was discharged to 0.3m from the height of 0.7m in each cycle, and the inflow water was fed to 0.7m from 0.3m, i.e., the inflow and outflow water amount per cycle was 100L.
The device operates: morning 6: the 00 aerator stops aeration, enters a precipitation stage, and precipitates for 1h,7:00 drain for 1h, stand by for 30min,8:30, water starts to be treated in one period, water is fed for 30min,9: after 00 times of water inflow, starting an aerobic aeration reaction for 300min, and 14: stopping aeration by the 00 aerator, and precipitating for 1h,15:00 drain for 1h, stand by for 30min,16:30 starts to feed water for a new cycle and so on. The automatic control is carried out by adopting a Kede timer (model: TW-K11), and each switch is opened at regular time. 3 cycles per day, 8 hours per cycle. To operate for one month. During the operation of the experimental device, when the MLSS is more than 4000mg/L, the sludge is discharged from the reactor, and the sludge discharge amount is the standard that the sludge concentration in the reactor is reduced to about 4000 mg/L.
The conventional detection index and analysis method are shown in table 4:
table 4 test items and analysis method
| Detecting items | Analysis method |
| COD | Potassium dichromate process |
| NH 4 + -N | Ultraviolet spectrophotometry |
| TN | Oxidation of potassium persulfate |
| TP | Spectrophotometry of ammonium molybdate |
| MLSS | Drying weighing method |
| MLVSS | Muffle furnace firing method |
| SV 30 | Sedimentation method |
Sludge reduction evaluation method
Total sludge yield per reactor = sludge in each reactor at the end of the experiment + sludge discharge per reactor during the experiment-sludge in each reactor at the beginning of the experiment (start of dosing)
Drug addition group sludge reduction = control group total sludge production-drug addition group total sludge production
Drug-added sludge reduction rate = drug-added sludge reduction rate/control group total sludge yield x 100%
Dosing group sludge reduction rate = dosing group sludge reduction rate/control group total sludge discharge x 100%
The microbial inoculum prepared in comparative examples 1 to 4, example 1 and examples 7 to 11 was subjected to the above test, and the results of the effluent quality detection are shown in Table 5:
TABLE 5 detection results of various indicators of effluent quality
As can be seen from Table 5, after the microbial inoculum prepared in example 1 is added, the removal rate of each index is obviously higher than that of the control group and comparative examples 1-4, which shows that the removal effect on pollutants such as COD is enhanced after the microbial inoculum is added, and the synergistic effect of the total allyloxy cucurbituril [6] uril potassium sulfate and the nano-scale calcium aluminum garnet powder can effectively promote the strengthening effect. The effect of example 8 is equivalent to that of example 1 and slightly better than that of example 7, and shows that the sclareol has good strengthening effect by taking sclareol as a liquid auxiliary material, and the existence of sclareol has synergistic strengthening effect, but the TP removing effect is not obviously improved. The effects of example 9 are better than those of example 1, example 10 and example 7 and example 11 than example 8, and the fact that the biological activity of the microbial inoculum can be effectively improved by adding the pummelo peel, so that the effect is enhanced.
The sludge reduction evaluation results are shown in table 6:
TABLE 6 sludge reduction test results
| Sample of | Emission reduction sludge/% | Yield-reduced sludge/% |
| Comparative example 1 | 30.42 | 50.13 |
| Comparative example 2 | 32.43 | 51.23 |
| Comparative example 3 | 30.12 | 53.21 |
| Comparative example 4 | 36.41 | 55.47 |
| Example 1 | 42.41 | 60.17 |
| Example 7 | 39.13 | 55.53 |
| Example 8 | 41.23 | 58.12 |
| Example 9 | 49.13 | 64.39 |
| Example 10 | 43.25 | 59.42 |
| Example 11 | 45.43 | 62.18 |
As can be seen from Table 6, the microbial inoculum prepared in example 1 is significantly higher in emission reduction and yield reduction than those of comparative examples 1-4, indicating that the synergistic effect of the microbial inoculum and the potassium salt of full allyl oxo-cucurbit [6] uril sulfate and the nano-scale lime-aluminum garnet powder can effectively improve the sludge reduction effect. The effect of example 8 is equivalent to that of example 1 and slightly better than that of example 7, and shows that the sclareol has good strengthening effect by taking sclareol as a liquid auxiliary material and has synergistic strengthening effect by the sclareol. The effects of example 9 are better than those of example 1, example 10 and example 7 and example 11 than example 8, and the fact that the biological activity of the microbial inoculum can be effectively improved by adding the pummelo peel, so that the effect is enhanced.
The conventional technology in the above embodiments is known to those skilled in the art, and thus is not described in detail herein.
The foregoing is merely illustrative of the present invention, and the present invention is not limited thereto, and any person skilled in the art will readily recognize that variations or substitutions are within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (7)
1. The application of full allyl oxo-cucurbit [6] uril potassium sulfate and nano-level calcium aluminum garnet powder in enhancing the action of microbial inoculum for strengthening sludge.
2. A microbial agent comprising:
the main material is composite bacteria including paracoccus denitrificans, clostridium paperlyticum, trichoderma harzianum, nitrifying bacteria, candida and bacillus amyloliquefaciens;
auxiliary materials including potassium salt of full allyl oxo cucurbit [6] uril sulfate, nano-level calcium aluminum garnet powder and peptone; the weight ratio of the main materials to the auxiliary materials is 1: 2-4;
the composite bacteria comprise, by weight, 9-12 parts of paracoccus denitrificans, 8-12 parts of clostridium paperlyticum, 6-9 parts of trichoderma harzianum, 5-8 parts of nitrifying bacteria, 4-6 parts of candida and 3-6 parts of bacillus amyloliquefaciens;
the mass ratio of the full allyl oxo-cucurbit [6] uril potassium sulfate, the nanoscale calcium aluminum garnet powder and the peptone in the auxiliary materials is 1:0.9 to 1.2:0.8 to 1.
3. Use of the microbial inoculum of claim 2 for enhancing activated sludge or sewage treatment.
4. The method for preparing the microbial inoculum of claim 2, comprising:
preparing a composite microbial bacterial liquid, namely respectively carrying out primary slant culture and secondary liquid culture on paracoccus denitrificans, clostridium paperlyticum, trichoderma harzianum, nitrifying bacteria, candida and bacillus amyloliquefaciens to obtain a microbial fermentation liquid, and then mixing to obtain the composite microbial bacterial liquid;
the auxiliary material is prepared by mixing full allyl oxo cucurbit [6] uril potassium sulfate, nano-level calcium aluminum garnet powder and peptone;
and (3) preparing a microbial agent, namely uniformly mixing the composite microbial liquid with auxiliary materials to obtain the microbial agent.
5. The method for preparing the microbial inoculum according to claim 4, which is characterized in that: the preparation method comprises the steps of adding 0.08-0.1% of canthaxanthin into the composite microbial liquid prepared from the composite microbial liquid.
6. The method for preparing the microbial inoculum according to claim 4, which is characterized in that: in the preparation of the compound microorganism bacterial liquid, each bacterial is cultivated until the bacterial number is 2-5 multiplied by 10 8 And each mL.
7. The use of the microbial inoculum in the sewage concentration treatment of reinforced activated sludge, which is characterized in that the use amount of the microbial inoculum is 1-5wt% of the total amount of treated sewage.
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