CN105505739B - A kind of cell culture apparatus - Google Patents
A kind of cell culture apparatus Download PDFInfo
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- CN105505739B CN105505739B CN201410490108.3A CN201410490108A CN105505739B CN 105505739 B CN105505739 B CN 105505739B CN 201410490108 A CN201410490108 A CN 201410490108A CN 105505739 B CN105505739 B CN 105505739B
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- positioning support
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/16—Apparatus for enzymology or microbiology containing, or adapted to contain, solid media
- C12M1/18—Multiple fields or compartments
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
Abstract
The invention discloses a kind of cell culture apparatus, it is related to photo-biological technical field, is invented the problem of relatively low for the utilization ratio of light in solution cell culture.A kind of cell culture apparatus, including：Support component；Adjusting part, the adjusting part is arranged in the support component, and the adjusting part includes top positioning support and pin positions support；Positioning component, when the positioning component is in lockup state, the positioning component can be such that the pin positioning support is relatively fixed with the top positioning support；Culture assembly, the culture assembly includes culture medium, top-hung fitting and pin and hangs fitting, the top-hung fitting is connected with the top positioning support, the pin hangs fitting and is connected with pin positioning support, the relative movement of the pin positioning support and the top positioning support can drive the pin to hang fitting and be relatively moved with the top-hung fitting, so as to change the angle of culture medium surface and horizontal plane.A kind of cell culture apparatus of the present invention is used for both culturing microalgae.
Description
Technical field
The present invention relates to photo-biological technical field, more particularly to a kind of cell culture apparatus.
Background technology
Microalgae refers to that photosynthesis can be carried out and only have that the small algae monoid of its form could be differentiated under the microscope,
Microalgae is nutritious, high financial profit, available for all many-sides such as food, medicine, bioenergy, the depollution of environment, animal feed.
The core of microalgae industry is large-scale production to obtain micro algae biomass, and low cost, the exploitation of high-efficient culture mode are rule
The key of modelling production.
There is a kind of Inserting-plate type microalgae semi-dry solid adherent culture device in the prior art, the device is utilized perpendicular to ground simultaneously
A series of culture plate cultivating microalgaes set in a determining deviation, by liquid distributor, collecting tank, reservoir and circulating pump etc. to culture
The microalgae supplying nutrition liquid cultivated on plate.The arrangement achieves the scattered utilization to light (particularly natural light).
However, said apparatus is in actual use, because the spacing and its angle of horizontal plane between culture plate are
It is changeless, due in one day not in the same time, and in not middle sun altitude and the sun side on the same day of synchronization
Parallactic angle can change, thus occur that sunlight does not impinge upon on culture plate and directly impinges upon the space between each culture plate daily
In situation, cause a large amount of wastes of luminous energy；And on the culture plate cell of local location have because light is too strong and becomes
It is yellow, have because light is not enough and it is slow-growing (illumination and the actual demand mismatch of cell that i.e. cell is obtained), cause luminous energy
Effective rate of utilization it is low.
The content of the invention
Embodiments of the invention provide a kind of cell culture apparatus, can improve the wasting phenomenon of luminous energy, improve the effective of light
Utilization rate, so as to improve the yield of microalgae.
To reach above-mentioned purpose, embodiments of the invention are adopted the following technical scheme that：A kind of cell culture apparatus, including：
Support component；Adjusting part, the adjusting part is arranged in the support component, and the adjusting part includes top positioning support
Position and support with pin, the pin positioning support can be relatively moved with the top positioning support；Positioning component, when the positioning component
During in lockup state, the positioning component can be such that the pin positioning support is relatively fixed with the top positioning support；Culture group
Part, the culture assembly includes culture medium, top-hung fitting and pin and hangs fitting, the culture medium respectively with top-hung fitting and pin
Outstanding fitting connection, the top-hung fitting is connected with the top positioning support, and the pin hangs fitting and is connected with pin positioning support,
When pin positioning support is with the top positioning support relative movement, the phase of the pin positioning support and the top positioning support
The pin can be driven to hang fitting and the top-hung fitting to movement to relatively move, so as to change culture medium surface and horizontal plane
Angle.
Top-hung fitting in cell culture apparatus provided in an embodiment of the present invention, culture assembly is determined with the top in adjusting part
Pin in position support connection, culture assembly hangs fitting and is connected with the pin positioning support in adjusting part, and culture medium connects with top-hung
Part and pin hang fitting connection, when carrying out cell culture, can be according to sun altitude and azimuthal change adjustment pin positioning
The relative position of support and top positioning support, so that adjusting pin hangs the relative position of fitting and top-hung fitting, and then makes culture be situated between
Pin now, can be positioned support by positioning component and be relatively fixed with top positioning support by matter horizontal by certain angle, so that
Realize the adjustment of the angle of culture medium surface and horizontal plane.Thus, can be according to solar space position (elevation angle, azimuth
Deng) change adjust the angle on culture medium surface and horizontal plane, so as to improve the wasting phenomenon of luminous energy, improve light
Effective rate of utilization, and then improve the yield of microalgae.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the accompanying drawing used required in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the general structure schematic diagram of cell culture apparatus of the embodiment of the present invention；
Fig. 2 is the culture assembly and the placement graph of a relation of adjusting part in cell culture apparatus of the embodiment of the present invention；
Fig. 3 is structural representation when two top positioning supports are intersecting in cell culture apparatus of the embodiment of the present invention；
Fig. 4 is the structural representation of the first positioning component in cell culture apparatus of the embodiment of the present invention；
Fig. 5 is the schematic diagram that adjusting part is parallelogram sturcutre in cell culture apparatus of the embodiment of the present invention；
Fig. 6 is the schematic diagram that adjusting part is trapezium structure in cell culture apparatus of the embodiment of the present invention；
Fig. 7 is the structural representation of second of positioning component in cell culture apparatus of the embodiment of the present invention；
Fig. 8 is the structural representation of the third positioning component in cell culture apparatus of the embodiment of the present invention；
Fig. 9 is the structural representation that top in cell culture apparatus of the embodiment of the present invention/upper anchor point of pin positioning support is suspension ring
Figure；
Figure 10 is that the structure that top in cell culture apparatus of the embodiment of the present invention/upper anchor point of pin positioning support is U-type groove is shown
It is intended to；
Figure 11 is that the structure that top in cell culture apparatus of the embodiment of the present invention/upper anchor point of pin positioning support is V-groove is shown
It is intended to；
Figure 12 is that the structure that top in cell culture apparatus of the embodiment of the present invention/upper anchor point of pin positioning support is rectangular channel is shown
It is intended to；
Figure 13 is that the structure that top in cell culture apparatus of the embodiment of the present invention/upper anchor point of pin positioning support is rectangular opening is shown
It is intended to；
Figure 14 is that the structure that top in cell culture apparatus of the embodiment of the present invention/upper anchor point of pin positioning support is circular port is shown
It is intended to；
Figure 15 is that top in cell culture apparatus of the embodiment of the present invention/upper anchor point of pin positioning support is variable spacing parallel four
The structural representation in side shape hole；
Figure 16 is that top in cell culture apparatus of the embodiment of the present invention/upper anchor point of pin positioning support is constant spacing parallel four
The structural representation in side shape hole；
Figure 17 is that the rotary shaft of transmission wheel in cell culture apparatus of the embodiment of the present invention is set along the outstanding fitting parallel direction of top/pin
The structural representation of culture assembly and liquid feeding assembly when putting；
Figure 18 is that the rotary shaft of transmission wheel in cell culture apparatus of the embodiment of the present invention is set along the outstanding fitting vertical direction of top/pin
The structural representation of culture assembly when putting；
Figure 19 is the bridging arrangement schematic diagram of single culture medium in cell culture apparatus of the embodiment of the present invention；
Figure 20 is the knot that top positioning support is transmission gear group and transmission hinge in cell culture apparatus of the embodiment of the present invention
Structure schematic diagram；
Figure 21 is the structural representation of the first liquid distributor in cell culture apparatus of the embodiment of the present invention；
Figure 22 is the structural representation of second of liquid distributor in cell culture apparatus of the embodiment of the present invention；
Figure 23 is the structural representation of the third liquid distributor in cell culture apparatus of the embodiment of the present invention；
Figure 24 is the structural representation of the liquid feeding assembly with EGR in cell culture apparatus of the embodiment of the present invention
Figure；
Figure 25 is general structure when cell culture apparatus of the embodiment of the present invention selects two groups of inoculation components and collection assembly
Schematic diagram.
Wherein：1- support components, 2- adjusting parts, 3- positioning components, 4- culture assembly, 5- bottoms connector, 6- liquid is supplied
To component, 7- inoculation components, 8- collection assemblies, 9- conveying assemblies, 11- support columns, 12- support frames, the positioning support of 21- tops, 22-
Pin positioning support, 23- sides connector, 31- first angle adjustment holes, 32- second angle adjustment holes, 33- keepers, 34- first
Fixture, the regulating parts of 35- first, the fixtures of 36- second, the regulating parts of 37- second, 41- culture mediums, 42- top-hung fittings, 43-
Pin hangs fitting, 44- transmission wheels, 61- liquid traps, 62- woven hoses, 63- liquid distributors, 64- EGRs, 71- classification inoculation apparatus, 72-
Mobile device, 81- collection devices are inoculated with, 82- collects mobile device, 91- extraction elements, 92- conveying devices, the positioning of 211- tops
Point, 212- transmission gear groups, 213- transmission hinges, 214- is raised, 221- pin anchor points, the elongated slot of the fixtures of 341- first,
The elongated slot of the fixtures of 361- second.
Embodiment
A kind of cell culture apparatus of the embodiment of the present invention is described in detail below in conjunction with the accompanying drawings.
In the description of the invention, it is to be understood that term " " center ", " on ", " under ", "front", "rear", " left side ",
The orientation or position relationship of the instruction such as " right side ", " vertical ", " level ", " top ", " bottom ", " interior ", " outer " are based on shown in the drawings
Orientation or position relationship, are for only for ease of the description present invention and simplify description, rather than indicate or imply signified device or
Element must have specific orientation, with specific azimuth configuration and operation, therefore be not considered as limiting the invention.
Fig. 1 and Fig. 2 is a specific embodiment of cell culture apparatus of the embodiment of the present invention, with reference to Fig. 1 and Fig. 2, this hair
Bright embodiment cell culture apparatus, including：Support component 1；Adjusting part 2, adjusting part 2 is arranged in support component 1, regulation
Component 2 includes top positioning support 21 and pin positioning support 22, and pin positioning support 22 can be relatively moved with top positioning support 21；Positioning
Component 3, when positioning component 3 is in lockup state, positioning component 3 can make pin positioning support 22 relatively solid with top positioning support 21
It is fixed；Culture assembly 4, culture assembly 4 includes culture medium 41, top-hung fitting 42 and pin and hangs fitting 43, culture medium 41 respectively with
Top-hung fitting 42 and pin hang fitting 43 and connected, and top-hung fitting 42 is connected with top positioning support 21, and pin hangs fitting 43 and positions branch with pin
Support 22 is connected, when pin positioning support 22 is relatively moved with top positioning support 21, pin positioning support 22 and top positioning support 21
Relative movement can drive pin to hang fitting 43 and be relatively moved with top-hung fitting 42, so as to change the folder of culture medium surface and horizontal plane
Angle.
In top-hung fitting 42 and adjusting part 2 in cell culture apparatus provided in an embodiment of the present invention, culture assembly 4
Top positioning support 21 is connected, and the pin in culture assembly 4 hangs fitting 43 and is connected with the pin positioning support 22 in adjusting part 2, cultivates
Medium 41 hangs fitting 43 with top-hung fitting 42 and pin and is connected, can be according to sun altitude and orientation when carrying out cell culture
The relative position of the change adjustment pin positioning support 22 and top positioning support 21 at angle, so that adjusting pin hangs fitting 43 and top-hung fitting
42 relative position, and then make culture medium 41 horizontal by certain angle, now, pin can be positioned by positioning component 3
Support 22 is relatively fixed with top positioning support 21, it is achieved thereby that the adjustment of the angle of culture medium surface and horizontal plane.Thus,
The angle on culture medium surface and horizontal plane can be adjusted according to the change at solar space position (elevation angle, azimuth etc.), from
And the wasting phenomenon of luminous energy is improved, the effective rate of utilization of light is improved, and then improve the yield of microalgae.
Wherein, as shown in Fig. 2 adjusting part 2 preferably includes two groups, the two ends of top-hung fitting 42 respectively with two groups of regulation groups
Two top positioning supports 21 in part 2 are connected, and the two ends that pin hangs fitting 43 are positioned with two pin in two groups of adjusting parts 2 respectively
Support 22 is connected, now, by adjusting two top positioning supports 21 and two pin positioning supports 22 respectively, culture can be caused to be situated between
The angle of matter surface and horizontal plane changes, it is also possible that culture medium surface rotates to an angle around vertical rotary shaft.
For example, when two pin positioning support 22 is respectively relative to two top positioning supports 21 and produces identical displacement, culture medium table
The angle of face and horizontal plane changes；When a pin positioning support 22 is relative to the generation displacement of another pin positioning support 22
When, culture medium surface can be around the rotary shaft rotation or torsion certain angle being vertically arranged.
Specifically, the space arrangement position of single top positioning support 21 is any, can be parallel to the ground or at an angle, phase
The relative position of the top of neighbour two positioning support 21 is any, can parallel, intersecting or antarafacial.Reference picture 2, two preferably adjacent top positioning
Support 21 is located in the same horizontal plane and arranged in parallel, and two pin positioning supports 22 also are located in same level and mutual
It is arranged in parallel, this set form is conducive to while installing more Multiple Shape identical culture medium 41；Reference picture 3, two tops are fixed
Position support 21 may be located in same level and one end is directly connected to, and the other end can be connected by pushing up connecting rod, and now two
Individual top positioning support 21 and the top triangle structure of connecting rod, preferably comprise isosceles triangular structure, this set form has
Beneficial to the stability that device is overall, compared with above-mentioned be arranged in parallel, the front and rear effect of blocking between culture medium reduces, culture medium
Photoreceiving surface is stronger, while the culture medium gross area is increased, can preferably be divided by way of reducing culture assembly interval
Light, realizes abundant effective utilization to luminous energy.
Support component 1 is used for branch support positioning support 21, as shown in Fig. 2 support component 1 can only include support column 11,
Support column 11 is located at the lower section of top positioning support 21 and preferably connection fixed thereto；Support column 11 and support beam, branch can also be included
Dagger 11 with support beam or by support beam group into support frame 12 be preferably fixedly connected, top positioning support 21 be fixed on support beam or
By support beam group into support frame 12 on.Reference picture 4, support component 1 preferably includes support column 11 and is fixedly installed on support
Support frame 12 on post 11, top positioning support 21 is fixed on support frame 12 and supported by support frame 12, under this mode, branch
The support force more equalization stable of 1 pair of top positioning support 21 of support component, so as to ensure that the stability of device.
When carrying out the angular adjustment of culture medium 41 by changing the position of pin positioning support 22, pin positioning support 22 exists
Change is horizontally and vertically subjected to displacement on direction, therefore, reference picture 4, in an embodiment of the present invention, positioning component 3
The first angle adjustment hole 31 being opened on support column 11, the second angle regulation being opened in pin positioning support 22 can be included
Hole 32 and keeper 33, when pin positioning support 22 is moved to precalculated position relative to top positioning support 21, keeper 33 can be according to
Secondary pass through is fixed after first angle adjustment hole 31 and second angle adjustment hole 32, so that by pin positioning support 22 relative to support group
Part 1 is fixed.First angle adjustment hole 31 is used to meet pin positioning support 22 vertical displacements change demand, second angle adjustment hole 32
For meeting its horizontal displacement change demand, when bipod positioning support 22 is relative to the position produced by support column 11 adjacent thereto
Phase shift can realize the regulation of the angle of culture medium surface and horizontal plane simultaneously, when bipod positioning support 22 is relative to adjacent thereto
Support column 11 produced by displacement it is different when, culture medium surface around the rotary shaft rotation being vertically arranged or can reverse certain
Angle；Culture assembly 4 is adjusted to after certain position, positioning component 3 is sequentially passed through first in lockup state, i.e. keeper 33
Angle adjustment holes 31 and second angle adjustment hole 32 are set and fixed, so that pin positioning support 22 be consolidated relative to support column 11
It is fixed, and then ensure that culture assembly 4 is fixed on new position.
In addition, when carrying out the angular adjustment of culture medium 41 by changing the position of pin positioning support 22, pin positioning branch
Support 22 also can an in the vertical direction be subjected to displacement change (now, the preferred surface of pin keeper is straight), therefore reference picture 4,
In a kind of embodiment of the present invention, using the first angle adjustment hole 31 being opened on support column 11, and when culture medium and water
Pin positioning support 22 can be achieved in vertical side into the second angle adjustment hole 32 in that pin positioning support 22 at 90 degree in plane
To movement, that is, have adjusted top positioning support 21 and pin positioning support 22 between distance, now the position of top-hung fitting 42 is motionless, pin
Outstanding fitting 43 produces displacement in a vertical and horizontal direction.When bipod positioning support 22 in the vertical directions movement identical position
During shifting, the regulation of the angle of culture medium surface and horizontal plane can be achieved, when bipod positioning support 22 in the vertical directions movement
During different displacement, culture medium surface around the rotary shaft rotation being vertically arranged or can reverse certain angle, and culture assembly 4 be adjusted
It is whole to after certain position, making positioning component 3 sequentially pass through first angle adjustment hole 31 in lockup state, i.e. keeper 33 and work as
Culture medium is set and fixed with the horizontal that second angle adjustment hole 32 at 90 degree, so that pin positioning is supported into 22 phases
Fixed for support column 11, and then ensure that culture assembly 4 is fixed on new position.
Wherein, keeper 33 preferably includes screw bolt and nut, when positioning component 3 is in lockup state, and bolt is through the
One angle adjustment holes 31 and second angle adjustment hole 32 are set, and are fixed by nut.
As shown in figure 4, first angle, which adjusts 31 holes and second angle adjustment hole 32, can be set to multiple apertures or single
Elongated hole, when being multiple apertures, keeper 33 is relatively stablized when fixing culture assembly 4 through aperture；When being single elongated hole,
The optional position in fixed culture assembly 4 of keeper 33 is continuous, easily along length after convenient regulation, but the fixation of keeper 33
The length direction in hole loosens, and is not sufficiently stable；Thus, it is preferable to first angle adjustment hole 31 is set to vertically to set one
Individual elongated hole, multiple apertures in the horizontal direction are set to by second angle adjustment hole 32.First angle adjustment hole 31 is set to one
During individual elongated hole, it can make it that the change of 22 in the vertical direction positions of pin positioning support is continuous, second angle adjustment hole 32 is set
During for multiple apertures, the fixing-stable of pin positioning support 22 can be caused, thus, when keeper 33 is fixed, can both be made
Pin positioning support 22 is continuous in the optional position of vertical direction, convenient regulation, in turn ensure that keeper 33 fix after stability,
So as to ensure the stability of device.
In order that the overall structure of adjusting part 2 is more consolidated, side connector 23 is set preferably in adjusting part 2,
The one end of side connector 23 is hinged with top positioning support 21, and the other end is hinged with pin positioning support 22, for convenience pin positioning support
Top positioning support 21, can be fixedly connected, at this point it is possible to by pulling side connector 23 come real by 22 regulation with support component 1
Existing two side legs positioning support 22 positions the movement of support 21 relative to top, so as to reach the purpose of adjustment culture assembly 4.
Wherein, in order that adjusting part is more consolidated in the support of vertical direction, two are set in preferably one group adjusting part
Individual side connector, thus, top positioning support 21, pin positioning support 22 and two side connectors 23 are then constituted with unstable
Property quadrilateral structure, reference picture 5 and Fig. 6 for example can be trapezoidal or parallelogram, when needing adjustment culture assembly 4,
Top 21 positions of positioning support are constant, and pin positioning support 22 and the solid line position from figure of side connector 23 are moved to dotted line position, its
In, the trapezoidal structure type as between stable triangle and unstable parallelogram, its unstability is not so good as
Parallelogram, parallelogram adjustment angle is bigger than trapezoidal, and the adjustment of culture assembly 4 needs to cater to different solar angles
Degree, you can the scope of adjustment should try one's best greatly, it is therefore preferable that top positioning support 21, pin positioning support 22 and two side connectors
23 constitute parallelogram linkage.
Reference picture 7, in another embodiment of the invention, positioning component 3 can also be realized by following structure：It is fixed
Hyte part 3 includes the first fixture 34 and the first regulating part 35, and the first fixture 34 is fixed relative to support component 1, and first consolidates
It can be the object beyond the present apparatus to determine part 34, or fixture (such as support column 11, support beam, the liquid collecting of device in itself
Device 61 etc.), one end of the first regulating part 35 is fixedly connected with side connector 23 or pin positioning support 22, when pin positioning 22 phases of support
When being moved to precalculated position for top positioning support 21, the other end of the first regulating part 35 is fixed on the first fixture 34, so that
Pin positioning support 22 is maintained relative to top positioning support 21 and vertically opened up on precalculated position, preferably the first fixture 34
There is the elongated slot 341 of the first fixture, one end that the first regulating part 35 is fixed on the first fixture 34 is preferably provided with screw thread.Positioning
Component 3 can be one group, now, need to only pull the first regulating part 35 to certain position and positioning component 3 is in lockup state,
Set threaded one end to set through the elongated slot 341 of the first fixture, and be fixedly connected by nut, just can be realized
The regulation of the angle of culture medium surface and horizontal plane, and culture medium surface can be made around the rotary shaft rotation being vertically arranged
Or certain angle is reversed, but can not individually adjust the angle of culture medium surface and horizontal plane.Therefore, positioning component 3 is preferably
Two groups, now, the first regulating part 35 of one of which positioning component 3 is pulled, or pull the first of two groups of positioning components 3 to adjust simultaneously
Save part 35 but pull displacement different, and positioning component 3 is in lockup state, that is, set threaded one end through the first fixture
Elongated slot 341 set, and be fixedly connected by nut, just can realize the tune of the angle of culture medium surface and horizontal plane
Section, and make culture medium surface around the rotary shaft rotation being vertically arranged or reverse certain angle；And two groups of positioning groups are pulled simultaneously
First regulating part 35 of part 3 simultaneously makes pulling displacement identical, is pulled to after certain position, positioning component 3 is in lockup state, i.e.,
If threaded one end is set through the elongated slot 341 of the first fixture, and is fixedly connected by nut, list just can be realized
Solely adjust the angle of culture medium surface and horizontal plane.It should be noted that the first regulating part 35 can also use other structures, example
Such as rope structure, as long as it can be fixed on the first fixture 34.
In addition, the structure of above two positioning component 3 can also have simultaneously, i.e. the first fixture 34, the first regulating part
35th, first angle adjustment hole 31, second angle adjustment hole 32 and keeper 33 can be set simultaneously, now, when regulating training
After the angle for supporting medium, pin positioning support 22 can be positioned simultaneously by two groups of different positioning components, thus may be such that
Positioning is more consolidated, and is not likely to produce and is rocked.
As shown in figure 8, in another embodiment of the invention, cell culture apparatus can also include bottom connector 5, bottom
The two ends of connector 5 are connected with two pin positioning supports 22 respectively.In the present embodiment, positioning component 3 includes the second fixture
36 and second regulating part 37, the second fixture 36 is fixed relative to support component 1, and the second fixture 36 can be beyond the present apparatus
Object, or the fixture (such as liquid trap 61, support column 11, support beam) of device in itself, the second regulating part 37
One end is fixedly connected with bottom connector 5, when pin positioning support 22 is moved to precalculated position relative to top positioning support 21, and second
The other end of regulating part 37 is fixed on the second fixture 36 so that pin positioning support 22 relative to top positioning support 21 maintain it is pre-
Positioning is put, and the elongated slot 361 of the second fixture is vertically offered on preferably the second fixture 36, and the second regulating part 37 is fixed
One end on the second fixture 36 is preferably provided with screw thread, this mode, only need to pull the second regulating part 37 to certain position
And positioning component 3 is in lockup state, that is, set threaded one end and set through the elongated slot 361 of the second fixture, and pass through
Nut is fixed, and just can realize the regulation of the angle of culture medium surface and horizontal plane.It should be noted that the second regulating part 37
Other structures, such as rope structure, as long as it can be fixed on the second fixture 36 can be used.
In the above-described embodiments, the elongated slot 361 of the fixture of elongated slot 341 and second of above-mentioned first fixture can also be used
Multiple holes for being vertically opened on the first fixture or the second fixture or other structures are replaced, as long as can make
First regulating part or the second regulating part in the vertical direction can be fixed to multiple positions.
As shown in Fig. 2 for convenience of scale cell culture is carried out, top positioning is supported on 21 along its length provided with multiple
Push up and be provided with multiple pin anchor points 221 along its length in anchor point 211, pin positioning support 22, top-hung fitting 42 is fixed by top
Site 211 is detachably connected with top positioning support 21, and it is detachable by pin anchor point 221 and pin positioning support 22 that pin hangs fitting 43
Connection, at this point it is possible to hang fitting 43 in two top positioning branch by changing the top-hung fitting 42 being connected with culture medium 41 and pin
Position in support 21 and two pin positioning supports 22, realizes regulation and the culture medium of the angle of culture medium surface and horizontal plane
Spacing regulation between 41, can also make culture medium surface around the rotary shaft rotation being vertically arranged or reverse certain angle.When
When culture assembly 4 includes multigroup, the top-hung fitting 42 of multigroup culture assembly 4 is individually fixed in multiple top anchor points 211, multigroup
The pin of culture assembly 4 hangs fitting 43 and is individually fixed in multiple pin anchor points 221, now, can be by changing top-hung fitting simultaneously
42 and pin hang the position of the residing top anchor point 211 of fitting 43 and pin anchor point 221, to realize between multigroup culture assembly 4
Spacing regulation.Top anchor point 211 and pin anchor point 221 can be the structure of entity or the mark of similar scale,
For example, when groove of the top anchor point 211 using entity, pin anchor point 221 can be to be drawn in pin positioning to support some on 22
Top-hung fitting 42, now, need to only be fixed in groove by position mark, and it is right in pin positioning support 22 that the outstanding fitting 43 of pin is ridden upon
On the position mark answered.
Wherein, top anchor point 211 and pin anchor point 221 can use entity structure, can also push up anchor point 211 using real
Body structure, pin anchor point 221 uses position mark, or can also push up anchor point 211 and use position mark, and pin anchor point 221 is adopted
Use entity structure.When pushing up anchor point 211 using entity structure, top anchor point 211 can be to be arranged in top positioning support 21
Groove, positioning hole or suspension ring, top-hung fitting 42 are rod-like structure, and top-hung fitting 42 is equipped with the top positioning support 21
In groove, positioning hole or suspension ring, preferably top-hung fitting 42 with top positioning support 21 it is vertical and be equipped with groove, positioning hole or
In suspension ring；When pin anchor point 221 is using entity structure, pin anchor point 221 can be to be opened in pin positioning to support recessed on 22
Groove, positioning hole or suspension ring.It is rod-like structure that pin, which hangs fitting 43, and pin hangs fitting 43 and is equipped with the recessed of pin positioning support 22
In groove, positioning hole or suspension ring, preferably the outstanding fitting 43 of pin is vertical with pin positioning support 22 and is equipped with groove, positioning hole or hangs
In ring.The form and length that top positioning support 21 positions support 22 from pin can be different.When top/pin anchor point 211/221 is recessed
During groove, top/pin hangs more convenient when fitting 42/43 is put into top/pin positioning support 21/22 or pulled down；When top/pin anchor point 211/
221 when being positioning hole, and top/pin hangs fitting 42/43 can be with the positioning 21/22 more stable cooperation of support of top/pin；Reference picture 9, when
When top/pin anchor point 211/221 is suspension ring, top/pin anchor point 211/221 is located at top/lower section of pin positioning support 21/22, constitutes
The structure of top/pin anchor point 211/221 is fixed and can support 21/ along top/pin positioning through fastener (such as bolt, figure not shown in)
22 movements, facilitate the spacing of top/pin anchor point 211/221 to adjust, that is, facilitate the spacing between culture assembly 4 to adjust.Wherein, such as
Shown in Figure 10-12, groove can be U-type groove, V-groove or rectangular channel, and reference picture 13-16, positioning hole is rectangular opening, parallel four side
Shape hole or circular port, can be respectively suitable for top-hung fitting of different shapes or pin hangs fitting.
When top/pin anchor point 211/221 is the positioning hole being arranged in top/pin positioning support 21/22, such as Figure 13 and 14
Shown, top/pin positioning support 21/22 can include the upper and lower two parts being detachably connected, and a positioning hole part is located at top/pin
The upper part of positioning support 21/22, another part is located at the lower part of top/pin positioning support 21/22, now, top/pin positioning branch
Support 21/22 upper and lower two-part two ends can be fixed respectively in forms such as bolt (not shown)s as shown in figure 13, be put into or
When dismounting top/pin hangs fitting 42/43, bolt is opened, upper part is removed, after the outstanding fitting 42/43 of top/pin is installed, then will
Upper and bottom section bolt etc. is fixed；The upper part of top/pin positioning support 21/22 can also be such as Figure 14 institutes with lower part
Show, being set to one end and being hinged, the other end is fixed in forms such as bolt (not shown)s, fitting is hanged when being put into or dismantling top/pin
When 42/43, bolt is opened, part is a little rotated around it with being articulated and connected for lower part to the direction away from lower part, top/pin
After outstanding fitting 42/43 is installed, upper part is a little rotated around it with being articulated and connected for lower part to the direction close to lower part,
Until upper part is closed with lower part, then fixed with bolt.
It can also be parallelogram Tiebar structure that top positioning support 21 and/or pin positioning, which support 22, and reference picture 15, top is fixed
Site 211 and pin anchor point 221 can for the formation of parallelogram pull bar parallelogram hole, now, top-hung fitting 42 and/
Or pin hangs fitting 43 and is put into parallelogram hole, when pulling or compressing parallelogram pull bar, between parallelogram hole
Away from adjustable, i.e. the spacing of anchor point is adjustable, thus realize top-hung fitting 42 and/or pin hang fitting 43 position it is adjustable.Further
Ground, reference picture 16 can also be fixedly connected with two fix bars along its length in the both sides of parallelogram pull bar, and two fix
Bar is be arranged in parallel, now, and top-hung fitting 42 and/or the outstanding fitting 43 of pin are put into parallelogram hole, and other positions are changed in extraction
The regulation to top-hung fitting 42 and/or the outstanding position of fitting 43 of pin can be achieved.
As shown in FIG. 17 and 18, the culture medium 41 in culture assembly 4 can also be tubular structure, top-hung fitting 42 and pin
Transmission wheel 44 is respectively equipped with outstanding fitting 43, the culture medium 41 of tubular structure is sheathed on top-hung fitting 42 and pin hangs fitting 43
In transmission wheel 44, drive device, such as motor (not shown), in cell culture are connected with least one transmission wheel 44
During, motor can drive transmission wheel 44 to rotate, and transmission wheel 44 can drive culture medium 41 around the circulation rotating of transmission wheel 44
Motion, reference picture 17, when the rotary shaft of transmission wheel 44 hangs the parallel direction of fitting 42/43 along top/pin to be set, it is possible to achieve culture
Medium 41 along perpendicular to top/pin hang the direction of fitting 42/43 and bypass top/pin hang fitting 42/43 the circulation rotating of transmission wheel 44 transport
Dynamic, making top that light is too strong and side to light, the bottom and shady face not enough with light is exchanged with each other respectively；With reference to Figure 18, work as biography
When sending the rotary shaft of wheel 44 to hang fitting 42/43 vertical direction along top/pin and set, it is possible to achieve culture medium 41 along parallel to top/
Pin hangs the circulation rotating motion in the direction of fitting 42/43, and the shady face for making the too strong side to light of light not enough with light is exchanged with each other,
So as to avoid, the local light in culture medium surface is too strong or deficiency, therefore, above two form can solve cell culture
The problem of light of culture medium 41 is uneven in journey, is conducive to improving utilization ratio of the frustule to light.To avoid culture medium
41 rotated under the drive of transmission wheel 44 during there is the situation for deviateing transmission wheel 44, preferably edge on culture medium 41
One week of tubular structure is provided with spaced hole (not shown), and one week of transmission wheel 44 (does not show provided with projection in figure
Go out), male cooperation is arranged in hole.
As shown in figure 19, in culture assembly 4 it is multiple that top-hung fitting 42 and pin, which hangs fitting 43, and culture medium 41 may be used also
Think the form alternately overlapped between multiple top-hung fittings 42 and the outstanding fitting 43 of pin, such as culture medium 41 can be with one end and outermost
Top-hung fitting 42 connect, the other end alternately bypasses each pin and hanged after fitting 43 and top-hung fitting 42 successively, outstanding with outermost pin
Fitting 43 is connected；Culture medium 41 can also one end be connected with outermost top-hung fitting 42, the other end alternately bypasses each successively
Pin is hanged after fitting 43 and top-hung fitting 42, is connected with outermost top-hung fitting 42；Culture medium 41 can be with one end and outermost
The pin of side hangs fitting 43 and connected, and the other end alternately bypasses each pin and hanged after fitting 43 and top-hung fitting 42 successively, with outermost pin
Outstanding fitting 43 is connected.During using these connected modes, gap is not present between culture medium 41, is not in that sunlight is impinged upon in gap
Situation, it is to avoid luminous energy waste.In addition, the two ends of culture medium 41 can connect to forming closed loop, top-hung fitting 42 and/or pin are outstanding
Can be equipped with transmission device (such as motor, transmission wheel, figure not shown in), for example, can be in each He of top-hung fitting 42 on fitting 43
Pin hangs is equipped with transmission wheel on fitting 43, and drive device, such as motor are connected with least one described transmission wheel (in figure not
Show), motor can drive the transmission wheel to rotate, so as to drive the cycle rotation of culture medium 41.Can so culture be set to be situated between
The too strong top of the light of matter 41 and side to light are exchanged with each other with the bottom of light deficiency and shady face respectively, it is to avoid culture medium table
Light is too strong or deficiency for face part, can effectively solve the problem of light of culture medium 41 is uneven in incubation, be conducive to carrying
Utilization ratio of the high frustule to light.
In addition, culture assembly 4 can also be following structure：Culture assembly 4 can also include the multiple top-hung being arranged alternately
Fitting 42 and multiple pin hang fitting 43, and each top-hung fitting 42 two pin adjacent thereto are respectively connected with culture between hanging fitting 43
Medium 41.Thus, no matter how sun altitude and solar azimuth change, and culture medium 41 is not impinged upon all without there is sunlight
The situation in the space between each culture medium is directly above impinged upon, a large amount of wastes of luminous energy can be avoided.
As shown in figure 20, in another embodiment of the invention, top positioning support 21 can also be following form：Including passing
Gear train 212 and the transmission hinge 213 being connected with transmission gear group 212 are sent, top-hung fitting 42 is arranged at transmission hinge 213
On, transmission hinge 213 is played a supporting role to top-hung fitting 42, with the rotation of transmission gear group 212, and transmission hinge 213 can
Drive top-hung fitting 42 to move in parallel, culture assembly 4 is transported to the both sides of device, is easy to " be inoculated with " and " collection " process.
To avoid being interval with multiple raised 214 on the play on transmission hinge 213 of culture assembly 4, transmission hinge 213,
Culture assembly 4 can be set to multigroup, at most provided with one group of culture assembly 4 between two neighboring raised 214, so as to by multigroup culture
Component 4 is separated from each other, and prevents it from contacting with each other, and influences cell growth.In addition, the bottom of hinge 213 contacted with top-hung fitting 42
Auxiliary support, such as support beam (not shown) are preferably added, preferably to bear the weight of culture assembly 4, dress is improved
The stability put.
As shown in figure 1, cell culture apparatus also include liquid feeding assembly 6, liquid feeding assembly 6 can to apposition growth in
Cell supply moisture, nutrients or bactericidal agent on culture medium 41, with reference to Fig. 8, liquid feeding assembly 6 preferably includes to connect successively
Logical liquid trap 61, woven hose 62 and liquid distributor 63, liquid distributor 63 is provided with fluid hole (not shown), fluid hole and transfusion
Pipe 62 is connected, and fluid hole can spray liquid to the culture medium.Further preferred fluid hole is located at the top of culture medium 41,
And the front or the back side or middle of culture medium can be located at, when carrying out cell culture, enter liquid distributor 63 from woven hose 62
Moisture, nutrients or bactericidal agent flowed out from fluid hole, and then make liquid distribution in culture medium surface；In addition, every can be real
The component for now making cell on culture medium 41 obtain moisture, nutrients or bactericidal agent by the mode such as spraying, spilling, drip, soak can also be made
For liquid feeding assembly 6.
Wherein, as shown in figure 21, liquid distributor 63 preferably unites two into one with the top-hung fitting 42 positioned at the top of culture medium 41,
The cavity being as opened in top-hung fitting 42 and the fluid hole being opened on the side wall of top-hung fitting 42, fluid hole connect with cavity
It is logical, now, space is not only saved, and also top-hung fitting 42 can realize the function of cloth liquid and suspension culture medium 41 simultaneously.
Reference picture 22 and 23, liquid distributor 63 can also be to set foraminate tubule, and aperture is connected with the inner chamber of tubule, aperture
For fluid hole, now, liquid distributor 63 can be located at the lower section of top-hung fitting 42, the top of culture medium 41 as shown in figure 22, and edge is located at
The top-hung fitting 42 on the top of culture medium 41 is arranged；The top of culture medium 41 can also be arranged in as shown in figure 23, and top-hung connects
Part 42 is the deformation of cylindricality, is placed in the both sides of culture medium 41.Moisture, nutrients or bactericidal agent in tubule can slowly flow through aperture
To culture medium surface, the growth of the nutrition of uniform and delicate, more conducively cell is provided for cell.
Reference picture 24, liquid feeding assembly 6 also include EGR 64, EGR 64 to realize liquid conveying and
The lifting of potential energy, such as all types of water pumps, EGR 64 is serially connected between liquid trap 61 and woven hose 62, and EGR 64 can
Liquid in liquid trap 61 is delivered in liquid distributor 63 by woven hose 62, liquid trap 61 is located at the lower section of culture medium 41,
It can collect from liquid distributor 63 but the liquid that is not utilized by the cells, and by not being utilized by the cells of flowing out of each culture medium 41
Liquid can be come together in same liquid trap 61；Also each independent liquid trap 61 can be respectively equipped with by culture medium 41 (in figure not
Show), each liquid trap 61 is directly or indirectly connected with EGR 64, i.e., the liquid in each liquid trap 61 flows directly into circulation dress
EGR 64 is flowed into after putting 64 or collecting again；If the amount of liquid that liquid distributor 63 is provided to culture medium 41 is smaller or just full
Sertoli cell demand, i.e., when flowing out culture medium 41 without the liquid not being utilized by the cells, then can omit liquid trap 61, EGR 64
Entrance communicated with external source fluid Supplying apparatus.
It is preferred that liquid feeding assembly 6 also include filter (such as micropore liquid filter, figure not shown in), filtering dress
Install and be placed between liquid trap 61 and EGR 64, to remove the germ that will enter in the liquid in EGR 64, training
Foster cell and insect pest.
In the embodiment shown in Figure 17, when the culture medium 41 in culture assembly 4 is tubular structure, its corresponding liquid
Body feeding assembly 6 can be for the structure shown in Figure 17, i.e. liquid feeding assembly 6 in liquid collecting slot form, collecting tank provided with moisture,
Collecting tank, can be arranged at pin and hang below fitting by nutrients or bactericidal agent, and culture medium and pin are hanged into fitting is connected one
End is stretched into collecting tank.Thus, as transmission wheel 44 drives the rotation of tubular culture medium 41, culture medium is recyclable to enter collection
Moisture, nutrients or bactericidal agent are absorbed in liquid bath.This is simple in construction, occupies little space and low manufacture cost.
As shown in figure 1, cell culture apparatus also includes inoculation component 7, inoculation component 7 preferably includes classification inoculation apparatus 71 and connect
Mobile device 72 is planted, inoculation mobile device 72 (such as motor, electric hoist, but not limited to this) can drive classification inoculation apparatus 71 relative
Moved in culture medium 41, classification inoculation apparatus 71 can be by spraying, spilling, drip, soak, smear and/or the mode such as suction filtration is by memory block or confession
The cell supplied to mouth is attached to the surface or inside of culture medium 41, specifically, and classification inoculation apparatus 71 has cell memory block
With cell supply mouth, it can contact or not contact with culture medium 41, the cell in cell memory block adheres to via cell supply mouth
In culture medium surface or inside, it can be realized through but not limited to following form：If cell supply mouth is in roller shape, roller surface
Alternately contacted with culture medium surface and cell memory block, so that the cell in memory block is attached to culture culture medium table
Face/and inside；For another example cell supply mouth is in brush, and memory block is communicated with supply mouth, and the cell in memory block can flow into brush confession
To mouth, supply mouth is contacted with culture medium 41 makes the cell in memory block be attached to culture medium surface/and inside；For another example cell
Supply mouth is that the cell in spout, memory block can flow into spout, and (i.e. spout need not be with training to the spraying cell of culture medium 41 for spout
Medium 41 is supported to contact), the cell in memory block is attached to culture medium surface/and inside.In addition, inoculation component 7 can be containing only
Classification inoculation apparatus 71, can now realize attachment of the cell to culture medium 41 by the movement of culture medium 41.
Cell culture apparatus also includes collection assembly 8, and collection assembly 8 includes collection device 81 and collects mobile device 82,
Collect mobile device 82 collection device 81 can be driven to be moved relative to culture medium 41, collection device 81 can by scraping, squeezing, blow,
The mode such as spray and/or punching makes cell detachment culture medium 41, and specifically, collector can be realized through but not limited to following form
Make to be attached to all or part of cell on culture medium 41 with culture medium 41 to depart from：If collector is laminar hard material
Material, contacts and relatively moves with culture medium surface, cell is peeled off with culture medium 41；If collector is that filament shape is soft
Property material, contacts and relatively moves with culture medium surface, cell is peeled off with culture medium 41；For another example collector is can
Spout, the preferred spout for blowing out high wind/and a small amount of drop are in slit-shaped, and spout can will be carefully without contact with culture medium 41
Born of the same parents blow off from culture medium 41.Mobile device is with mobile device in above-mentioned inoculation group part 7.In addition, collection assembly 8 can be containing only receipts
Storage, can now realize the disengaging of cell by the movement of culture medium 41.It is preferred that collection assembly 8 in also contain access
Device, with hold with culture medium 41 depart from cell.
Above-mentioned inoculation component 7 and collection assembly 8 can use a variety of arrangement forms as needed, for example, working as culture medium
Relatively thin, it is only necessary to when being inoculated with to the side surface of culture medium one, inoculation component 7 and collection assembly 8 can select one group respectively,
And it can will be inoculated with component 7 and collection assembly 8 is respectively arranged at the left and right sides (as shown in Figure 1) of support component 1 or set
In the same side of support component 1；When culture medium is thicker, it is necessary to when the both side surface to culture medium is inoculated with, be inoculated with
Component 7 and collection assembly 8 can select two groups respectively, and two groups of inoculation components 7 are respectively arranged at the left and right two of support component
Side, two groups of collection assemblies 8 are respectively arranged at the left and right sides (as shown in figure 25) of support component 1, now, can pass through support component
The inoculation component 7 of 1 left and right sides is inoculated with to two surfaces of culture medium, and can pass through the left and right sides of support component 1
Collection assembly 8 cell on two surfaces of culture medium is collected.
Cell culture apparatus also includes conveying assembly 9, and conveying assembly 9 includes extraction element 91 and conveying device 92, extracts
Culture assembly 4 can be lifted or put down by device 91, and conveying device 92 can drive extraction element 91 to be moved relative to adjusting part 2,
Extraction element 91 can be realized through but not limited to following form：If extraction element 91 can be chain thing, one end of chain in pincers, hook,
Claw-like, the other end is collapsible (such as being realized by electric hoist, pulley, but not limited to this) so as to " lifting " or " putting down " culture group
Part 4 (also includes the liquid distributor 63 in liquid feeding assembly 6) sometimes in culture assembly 4, and by being connected with mobile device with reality
Movement between each anchor point, inoculation component 7 and the collection assembly 8 of present top/pin positioning support, to complete cell inoculation, cell
The process such as collection and the adjustment of the spacing of culture medium 41.In addition, conveying assembly 9 can now pass through culture containing only extraction element 91
The movement of medium 41 collect realizing cell inoculation, cell and the spacing of culture medium 41 adjustment etc..
In the description of this specification, specific features, structure, material or feature can be in any one or more realities
Apply and combined in an appropriate manner in example or example.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (25)
1. a kind of cell culture apparatus, it is characterised in that including：
Support component；
Adjusting part, the adjusting part is arranged in the support component, and the adjusting part includes top positioning support and pin
Positioning support, the pin positioning support can be relatively moved with the top positioning support；It is square along its length in the top positioning support
To provided with multiple top anchor points, the pin positioning is supported is provided with multiple pin anchor points along its length；
Positioning component, when the positioning component is in lockup state, the positioning component can make the pin positioning support and institute
Top positioning support is stated to be relatively fixed；
Culture assembly, the culture assembly includes culture medium, top-hung fitting and pin and hangs fitting, the culture medium respectively with top
Outstanding fitting and pin hang fitting connection, and the top-hung fitting is detachably connected by the top anchor point with top positioning support, described
Pin hangs fitting and is detachably connected by the pin anchor point and pin positioning support, and branch is positioned with the top when pin positioning is supported
During support relative movement, the relative movement of pin positioning support and the top positioning support can drive the outstanding fitting of the pin with it is described
Top-hung fitting is relatively moved, so as to change the angle of culture medium surface and horizontal plane.
2. cell culture apparatus according to claim 1, it is characterised in that the adjusting part includes two groups, the top
The two ends of outstanding fitting be connecteds with two top positioning supports in two groups of adjusting parts respectively, the two ends of the outstanding fitting of the pin respectively with
Two pin positioning support connection in two groups of adjusting parts.
3. cell culture apparatus according to claim 2, it is characterised in that two top positioning supports are located at same water
In plane and arranged in parallel, two pin positioning supports are located in the same horizontal plane and arranged in parallel.
4. cell culture apparatus according to claim 2, it is characterised in that two top positioning supports are located at same water
Plane is interior and one end is directly connected to, and the other end is connected by pushing up connecting rod, two top positioning supports and the top connecting rod
Triangle structure.
5. cell culture apparatus according to claim 1, it is characterised in that the top positions support phase for the support
Component is fixed.
6. cell culture apparatus according to claim 5, it is characterised in that at least include one in adjusting part described in one group
Individual side connector, described side connector one end is hinged with the top positioning support, and the other end is hinged with pin positioning support.
7. cell culture apparatus according to claim 6, it is characterised in that adjusting part described in one group includes two sides
Connector, the top positioning support, pin positioning support and two side connectors constitute parallelogram linkage.
8. cell culture apparatus according to claim 5, it is characterised in that the positioning component includes being opened in the branch
The first angle adjustment hole of support component, the second angle adjustment hole and keeper being opened in the pin positioning support, work as institute
State pin positioning support phase for it is described top positioning support be moved to precalculated position when, the keeper can sequentially pass through first angle
It is fixed after adjustment hole and second angle adjustment hole, so that pin positioning support phase be fixed for the support component.
9. the cell culture apparatus according to claim 6 or 7, it is characterised in that the positioning component, which includes first, to be fixed
Part and the first regulating part, first fixture are fixed relative to the support component, one end and side of first regulating part
Connector or pin positioning support are fixedly connected, and precalculated position is moved to for the top positioning support when the pin positions support phase
When, the other end of first regulating part is fixed on first fixture, so that the pin positions support phase for the top
Positioning support maintains precalculated position.
10. the cell culture apparatus according to any one of claim 2~8, it is characterised in that also including bottom connector,
The two ends of the bottom connector are connected with two pin positioning support in two groups of adjusting parts respectively.
11. cell culture apparatus according to claim 10, it is characterised in that the positioning component includes the second fixture
With the second regulating part, second fixture is fixed relative to the support component, one end of second regulating part with it is described
Bottom connector is fixedly connected, when the pin, which positions support phase, is moved to precalculated position for the top positioning support, and described the
The other end of two regulating parts is fixed on second fixture, so that the pin positions support phase for the top positioning support dimension
Hold in precalculated position.
12. cell culture apparatus according to claim 1, it is characterised in that the top anchor point is to be arranged at the top
Groove, positioning hole or suspension ring in positioning support, the top-hung fitting is rod-like structure, and the top-hung fitting is equipped with institute
In groove, positioning hole or the suspension ring for stating top positioning support；
And/or the pin anchor point is groove, positioning hole or the suspension ring being opened in the pin positioning support, the pin hangs fitting
For rod-like structure, the pin hangs fitting and is equipped with groove, positioning hole or the suspension ring for positioning support in the pin.
13. according to cell culture apparatus according to any one of claims 1 to 8, it is characterised in that the culture medium is cylinder
Shape structure, the top-hung fitting and pin, which hang, is respectively equipped with transmission wheel on fitting, the culture medium two ends difference of the tubular structure
It is sheathed on top-hung fitting and pin is hanged in the transmission wheel of fitting, the transmission wheel can drives the culture medium rotary motion.
14. cell culture apparatus according to claim 13, it is characterised in that along tubular structure on the culture medium
Spaced hole is provided with one week, one week of the transmission wheel is provided with projection, and the male cooperation is arranged in the hole.
15. cell culture apparatus according to claim 1, it is characterised in that the culture assembly includes what is be arranged alternately
Multiple top-hung fittings and multiple pin hang fitting, and each top-hung fitting two pin adjacent thereto are respectively connected between hanging fitting
Culture medium.
16. cell culture apparatus according to claim 1, it is characterised in that the culture assembly connects including multiple top-hung
Part and pin hang fitting, and described culture medium one end is connected with outermost top-hung fitting, and the other end alternately bypasses each pin and hanged successively
After fitting and top-hung fitting, hang fitting with outermost pin and be connected；
Or the culture assembly includes multiple top-hung fittings and pin hangs fitting, described culture medium one end connects with outermost top-hung
Part is connected, and the other end alternately bypasses each pin and hanged after fitting and top-hung fitting successively, is connected with outermost top-hung fitting；
Or the culture assembly includes multiple top-hung fittings and pin hangs fitting, described culture medium one end hangs with outermost pin to be connect
Part is connected, and the other end alternately bypasses each pin and hanged after fitting and top-hung fitting successively, and hanging fitting with outermost pin is connected.
17. cell culture apparatus according to claim 16, it is characterised in that the two ends of the culture medium are connected to form
Closed loop, each the top-hung fitting and pin hang is equipped with transmission wheel on fitting, and driving dress is connected with least one described transmission wheel
Put, the drive device can drive the transmission wheel to rotate, so as to drive the culture medium cycle rotation.
18. according to cell culture apparatus according to any one of claims 1 to 8, it is characterised in that the top positioning support bag
Transmission gear group and the transmission hinge being connected with transmission gear group are included, the top-hung fitting is arranged at the transmission hinge
On, with the rotation of the transmission gear group, the transmission hinge can drive the top-hung fitting to move.
19. cell culture apparatus according to claim 18, it is characterised in that be interval with the transmission hinge multiple
Projection, the culture assembly is multigroup, and culture assembly described in one group is at most provided between the two neighboring projection.
20. cell culture apparatus according to claim 13, it is characterised in that also including liquid feeding assembly, the liquid
Body feeding assembly includes collecting tank, and setting in the collecting tank is stretched into one end of the culture medium.
21. cell culture apparatus according to claim 1, it is characterised in that also including liquid feeding assembly, the liquid
Feeding assembly includes liquid trap, woven hose and the liquid distributor being sequentially communicated, and the liquid distributor is provided with fluid hole, the fluid hole
Connected with the woven hose, the fluid hole can spray liquid on the culture medium.
22. cell culture apparatus according to claim 21, it is characterised in that the liquid feeding assembly also includes circulation
Device, the EGR is serially connected between the liquid trap and woven hose, and the EGR can be by the liquid in liquid trap
It is delivered to by woven hose in liquid distributor, the liquid trap is located at the lower section of the culture medium.
23. cell culture apparatus according to claim 1, it is characterised in that also including inoculation component, the inoculation component
Including classification inoculation apparatus and inoculation mobile device, the inoculation mobile device can drive the classification inoculation apparatus to be situated between relative to the culture
Matter is moved, the classification inoculation apparatus can spray, spill, drip, soak, smear and/or suction filtration by way of cell is attached to culture medium
On.
24. cell culture apparatus according to claim 1, it is characterised in that also including collection assembly, the collection assembly
Including collection device and collection mobile device, the collection mobile device can drive the collection device to be situated between relative to the culture
Matter is moved, and the collection device can make cell detachment culture medium by way of scraping, squeezing, blow, spray and/or rush.
25. cell culture apparatus according to claim 1, it is characterised in that also including conveying assembly, the conveying assembly
Including extraction element and conveying device, culture assembly can be lifted or put down by the extraction element, and the conveying device can drive
Extraction element is moved relative to the adjusting part.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410490108.3A CN105505739B (en) | 2014-09-23 | 2014-09-23 | A kind of cell culture apparatus |
| PCT/CN2015/089078 WO2016045497A1 (en) | 2014-09-23 | 2015-09-07 | Cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410490108.3A CN105505739B (en) | 2014-09-23 | 2014-09-23 | A kind of cell culture apparatus |
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| Publication Number | Publication Date |
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| CN105505739A CN105505739A (en) | 2016-04-20 |
| CN105505739B true CN105505739B (en) | 2017-10-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410490108.3A Active CN105505739B (en) | 2014-09-23 | 2014-09-23 | A kind of cell culture apparatus |
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| WO (1) | WO2016045497A1 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108485968B (en) * | 2018-04-28 | 2021-11-19 | 刘姣姣 | Novel cell stable culture equipment convenient to observe |
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|---|---|---|---|---|
| CN101405385A (en) * | 2006-02-21 | 2009-04-08 | 亚利桑那州立大学董事会，代表亚利桑那州立大学法人团体利益 | Photobioreactor and uses therefor |
| CN102373156A (en) * | 2010-08-10 | 2012-03-14 | 中国科学院青岛生物能源与过程研究所 | Half-dry solid state cultivation method used for industrial production of microalgae |
| CN102465088A (en) * | 2011-09-07 | 2012-05-23 | 浙江齐成碳能科技有限公司 | Reaction zone structure of light-energy living being culture system and method for culturing light-energy living beings |
| CN103289888A (en) * | 2012-03-01 | 2013-09-11 | 中国科学院青岛生物能源与过程研究所 | Inserting-plate type microalgae semi-dry solid adherent culture device |
| CN103289887A (en) * | 2012-03-01 | 2013-09-11 | 中国科学院青岛生物能源与过程研究所 | Half-dry solid-state adherent culture device for microalgae industrial production |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2007217821B2 (en) * | 2006-02-21 | 2012-06-14 | The Arizona Board Of Regents, A Body Corporate Acting On Behalf Of Arizona State University | Photobioreactor and uses therefor |
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2014
- 2014-09-23 CN CN201410490108.3A patent/CN105505739B/en active Active
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2015
- 2015-09-07 WO PCT/CN2015/089078 patent/WO2016045497A1/en active Application Filing
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101405385A (en) * | 2006-02-21 | 2009-04-08 | 亚利桑那州立大学董事会，代表亚利桑那州立大学法人团体利益 | Photobioreactor and uses therefor |
| CN102373156A (en) * | 2010-08-10 | 2012-03-14 | 中国科学院青岛生物能源与过程研究所 | Half-dry solid state cultivation method used for industrial production of microalgae |
| CN102465088A (en) * | 2011-09-07 | 2012-05-23 | 浙江齐成碳能科技有限公司 | Reaction zone structure of light-energy living being culture system and method for culturing light-energy living beings |
| CN103289888A (en) * | 2012-03-01 | 2013-09-11 | 中国科学院青岛生物能源与过程研究所 | Inserting-plate type microalgae semi-dry solid adherent culture device |
| CN103289887A (en) * | 2012-03-01 | 2013-09-11 | 中国科学院青岛生物能源与过程研究所 | Half-dry solid-state adherent culture device for microalgae industrial production |
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| Publication number | Publication date |
|---|---|
| CN105505739A (en) | 2016-04-20 |
| WO2016045497A1 (en) | 2016-03-31 |
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