CN105474826B - A kind of method for promoting intelligence development seed to sprout - Google Patents
A kind of method for promoting intelligence development seed to sprout Download PDFInfo
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- CN105474826B CN105474826B CN201510875949.0A CN201510875949A CN105474826B CN 105474826 B CN105474826 B CN 105474826B CN 201510875949 A CN201510875949 A CN 201510875949A CN 105474826 B CN105474826 B CN 105474826B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention discloses a kind of method for promoting intelligence development seed to sprout.5 selection of the method for the present invention including the collecting period, seed processing early stage, vernalization, type of seeding and seedling management steps：Intelligent promoting fruit is plucked in early June to the middle ten days, after-ripening 35 days, removes pulp and flat kind, with the 48h of vernalization 12 at 0.005 0.030g/ml 28 30 DEG C of NaOH solution, after clear water rinses 35 times, is mixed with clean fine sand and intelligence development seed, ratio 1:1, the nursery in sand bed under suitable humiture.Such a method enables to intelligence development seed germination rate to reach more than 80%, and germination rate improves 6.25%, sprouts cycle time 10 days.The inventive method has simple to operate, and cost is low, easy to spread, has established good basis for the propagation in scale of intelligence development seedling, has had good economic benefit and social benefit.
Description
Technical field
The invention belongs to field of plant growing technology, and in particular to a kind of method for promoting intelligence development seed to sprout.
Background technology
Intelligence development (Alpinia oxyphyla Miq.) be zingiberaceous plant intelligence development edge dry mature fruit, alias intelligence development son,
Intelligence benevolence, to go through version《Pharmacopoeia of People's Republic of China》Recorded.With warm kidney astringent sperm contracting urine, warm spleen stops the effect of saliva is taken the photograph in gulf.With
In treatment enuresis with renal asthenia, frequent urination, seminal emission is white only, and malaria lets out gulf, the disease such as crymodynia, the more salivas of mouth in abdomen；Main product is wide in China
, also there is cultivation in east and Hainan Province on Fujian, Guangxi, Yunnan and other places.
Because intelligence development has the characteristic of extensive pharmacological action and its integration of drinking and medicinal herbs, medicine, the health care food of intelligence development in recent years
Product and food such as sugar-reducing comfort pill, cerebral tonic pill, Suoquan Wan, dog kidney intelligence development fruit capsule and your abundant strong intelligence development fruit piece etc. have tens of kinds
Product is come out one after another, and fructus alpiniae oxyphyllae consumption figure promotes greatly the area of artificial cultivation intelligence development to expand rapidly.Kind is bred using intelligence development cane
Seedling influences the yield of next year fructus alpiniae oxyphyllae, and the big survival rate of intelligence development cane demand is low, can not meet the market demand；Intelligence development
Grain weight is big, and every fruit there are about seed 20-25 grains, is especially suitable for for breeding seedling, but intelligence development seed has maturity
Factor, these factors such as not enough, germination rate is low, it is long to sprout the cycle, emergence is irregular seriously restrict scale and carry out intelligence development seed
Breed seedling.
The content of the invention
For problem above, inventor combines intelligence development seed biological features, has carried out a large amount of promotion intelligence development seeds and has sprouted
Experimental work, formulate the technical parameter of intelligence development picking fruit, soak seed using NaOH solution, the method can promote intelligence development
Seed is sprouted, and shortens seed and sprouts the cycle, and synergy is notable, easy to operate.
In consideration of it, it is an object of the invention to provide a kind of method for promoting intelligence development seed to sprout, for the kind of intelligence development seed
Seedling breeds offer technical support.
A kind of method for promoting intelligence development seed to sprout provided by the invention, comprises the steps：
(1) the intelligence development fruit of harvesting ripe：Reach the intelligence development fruit of standard of plucking, cut with scissors together with fruit ear；
(2) seed processing early stage：The fruit ear of step (1) harvesting is stacked in the cool so that Fruit anatomy 5-7 days；Will
By the intelligence development fruit of Aging storage, pulp, pericarp, decontamination, flat kind being removed, takes full seed, cleaned, shady place is dried,
It is standby；
(3) vernalization：The intelligence development seed dried is immersed in 12- in the NaOH solution that concentration is 0.005-0.030g/ml
48h, soaking temperature are 28-30 DEG C；
(4) sow：The seed soaked through NaOH solution is rinsed with clear water, is mixed with fine sand and intelligence development seed；By fine sand and
The mixture of seed is uniformly sprinkling upon on smooth casting bed, is then sprinkling decomposed coconut palm chaff；Made with the uniform spray water of sprayer
Obtain casting bed and keep moistening；Control humiture sprouts seed：30-33 DEG C of daytime, 24-26 DEG C of evening temperature, humidity 80%-
85%.
In the step (1), the standard for reaching the ripe intelligence development fruit of harvesting is：Intelligence development fruit surface is yellow (yellow by blue-stain
Green), acid foot；Single fringe fruit number reaches more than 42；Single fringe fruit fresh weight reaches more than 80g；Simple grain fruit weight
Reach more than 1.8g；Dry/fresh weight ratio of fruit is more than 0.34, and thousand grain weigth reaches more than 11g；TTC methods determine seed vitality
Index reaches 85%；In the standard detecting method, the intelligent promoting fruit randomly choosed in fruit planting area to be plucked is implemented as detecting
Material.
In the step (1), reach the selection of intelligence development fruit maturation collecting period：Plucking time is 10 days to 6 annual June
The moon 20.
Meanwhile, it is necessary to select the intelligence development fruit that fruit ear is long, fruit grain is big in the selection of intelligence development fruit.
In the step (3), preferably, NaOH solution concentration is 0.010-0.025g/ml, 0.015- is more elected as
0.020g/ml, optimal is 0.015g/ml；
Preferably, the soak time in NaOH solution is 12-36h, more preferably 24-36h, optimal is 36h.
In the above method, in the step (4), the part by weight of clean fine sand and the mixing of intelligence development seed is 1:1.
In the step (4), the purpose that clear water rinses is to rinse the NaOH solution of the surface of the seed well, typically rinses 3-
5 times.
In the step (4), fine sand used, granularity is the fine sand by 10 mesh sieves.Fine sand need to be eluriated totally with water, and
Be exposed to the sun 24h under the sun, makes it without pathogenic bacteria and the harmful chemical component for hindering seed to sprout.
In the above method, in the step (2), pulp, pericarp, decontamination, flat kind are removed, takes the method for full seed can
Think：Intelligence development fruit is pushed away and put together, spreading a small amount of water, to macerate pericarp rotten；Then trampled repeatedly with pin and macerate rotten intelligence development fruit,
Eluriated with water, drift go pulp, pericarp, decontamination, flat kind, take sink to the bottom seed both full seed.
The present invention has advantages below：
1. significantly improve intelligence development seed germination rate：The present invention handles intelligence development kind by optimizing NaOH concentration and its soak time
Son, intelligence development seed germination rate can be significantly improved, save intelligence development seed resource, improved economic benefit.
2. shortening intelligence development seed sprouts the cycle：By means of the invention it is possible to which shortening intelligence development seed sprouts the cycle, there is operation letter
Single, cost is low, easy to spread, and good basis has been established for the propagation in scale of intelligence development seedling, have good economic benefit and
Social benefit.
Embodiment
In order to be better understood from the present invention, with reference to specific embodiment, the present invention is described further.
Material used, reagent, unless otherwise specified, are commercially obtained in following embodiments.
Embodiment 1, the determination of intelligence development seed collection phase
Intelligence development Demonstration Base in main producing region Hainan Province Qiong Zhongxian Hong Mao towns of intelligence development, respectively May 15, May 30,
June 15, four-stage collection on June 25 intelligence development seed, each 4 parts of phase acquisition (every part of 1000 fruits), take back experiment
Room observation intelligence development fruit fruit colour change, measure fruit dry weight and fresh weight of plant seedlings ratio, thousand grain weigth, Seed Integrated vigor Index (TTC methods).
Fruit dry weight and fresh weight of plant seedlings is than measure：100 3 parts of intelligence development fruits are taken at random, is equably layered in sample box, is put into baking oven,
18h is dried at 103 ± 2 DEG C, is immediately placed in drying basin after taking-up, is weighed after being cooled to room temperature.Fruit dry weight and fresh weight of plant seedlings ratio=drying
Sample weight before sample weight/baking afterwards.
Mass of 1000 kernel determines：1000 seeds are taken at random, determine its quality, 3 repetitions.
TTC is dyed：By GB/T3543.7-1995《Crop seeds inspection procedure-sundry item is examined》Defined biochemistry
(tetrazolium) method (TTC methods) determines seed vigor, is 1 repetition with 100, totally 12 repetitions, average.By 1200 benefits
Intelligence seed is put into triangular flask, is contained into distilled water, liquid level was not had seed, is soaked 24h at room temperature.TTC pH7.8 phosphorus
Acid buffer is configured to 1.0% solution, and lucifuge is stored in brown bottle.Seed is cut into two along axis with blade during experiment
Half, it is put into 25ml beaker, adds a certain amount of 1.0%TTC solution, be completely soaked embryo.Water-bath keeps 30 DEG C of constant temperature, dye
Color 3h observes staining conditions, and is not dyed more than 1/2, completely by normal dyeing, part dyeing but radicle top or cotyledon top
Do not dye and count respectively.
The intelligence development fruit and seed biological features of 1. different collecting periods of table compares
The intelligence development seed vigor of 2. different collecting periods of table compares
Note：Normal dyeing：Seed can be grown to normal seedling after sprouting after representing；
Part dyeing but radicle top or cotyledon top are not dyed to be represented more than 1/2：Abnormally seedling；Do not dye completely：
Represent blind seed.
Shown by intelligence development fruit and seed the biological property measure of different collecting periods：June 10 and June 20 pluck
Fruit dry weight and fresh weight of plant seedlings ratio, thousand grain weigth and the seed vitality of intelligence development seed reach maximum, and tend towards stability (specifically see
Tables 1 and 2).Show intelligence development later on June 10 fruit ability full maturity, the nursery of high quality can just be obtained by now plucking fruit
Seed.
Accordingly, it is determined that the standard for reaching the ripe intelligence development fruit of harvesting is：Intelligence development fruit surface is yellow (yellow green) by blue-stain,
Acid foot；Single fringe fruit number reaches more than 42；Single fringe fruit fresh weight reaches more than 80g；Simple grain fruit weight reaches
More than 1.8g；Dry/fresh weight ratio of fruit is more than 0.34, and thousand grain weigth reaches more than 11g；TTC methods determine Seed Integrated vigor Index
Reach 85%.
Embodiment 2:Differing maturity seed germination rate determines
Intelligence development Demonstration Base in main producing region Hainan Province Qiong Zhongxian Hong Mao towns of the intelligence development described in embodiment 1, respectively takes four
The intelligence development fruit of collecting period (May 20, May 30, June 10 and June 20).Show according to the method detection of embodiment 1,
The fruit of June 10 and harvesting on June 20 reaches ripeness standard：Intelligence development fruit surface is yellow (yellow green) by blue-stain, acid foot；
Single fringe fruit number reaches more than 42；Single fringe fruit fresh weight reaches more than 80g；Simple grain fruit weight reaches more than 1.8g；Fruit
Real dry/fresh weight ratio is more than 0.34, and thousand grain weigth reaches more than 11g；TTC methods measure Seed Integrated vigor Index reaches 85%.
May 20, the fruit of harvesting on May 30 are not up to above-mentioned standard.
Cut, stacked in the cool together with fruit ear with scissors during the picking fruit for carrying out following sprouting experiments so that
Fruit anatomy 5-7 days；Intelligence development fruit is stacked upon, spreading a small amount of water, to macerate pericarp rotten, then trampled repeatedly with pin macerate it is rotten
Intelligence development fruit, is eluriated with water, and pulp, pericarp are removed in drift, is removed impurity, is taken and sink to the bottom seed, is cleaned, shady place is dried.
The seed in four periods by above-mentioned processing takes 1000 respectively, respectively by the fine sand and kind of the clean mesh sieve of mistake 10
The mixture of son, which is uniformly sprinkling upon, (vinyl disc bear, to guarantee unnecessary water the vinyl disc (sand bed) for being covered with fine sand is inner
Point ooze out), casting bed thickness 8cm, then surface spread the thick decomposed coconut palm chaffs of one layer of about 1cm, watering until bottom there is water to ooze out for
Only, vinyl disc is put into incubator, make it that casting bed keeps moistening with the uniform spray water of sprayer, 32 DEG C of design temperature daytime,
24 DEG C of evening temperature, humidity 80%, each processing set 4 repetitions, and culture calculates germination rate after 40 days.Germination rate=(sprout
The total seed number of seed number ÷ samples) × 100%.
The intelligence development percentage of seedgermination of 3. different collecting periods of table compares
| Pluck the date | May 20 | May 30 | June 10 | June 20 |
| Germination rate (%) | 9.21±1.22 | 28.13±2.71 | 75.56±2.18 | 77.14±2.29 |
As a result as shown in table 3, relatively shown by the intelligence development percentage of seedgermination of different collecting periods：With prolonging for collecting period
Late, intelligence development seed germination rate gradually steps up.The seed germination rate plucked after June 10 reaches without significant difference
More than 75.56%.It is indicated above that the maturity of collecting period and seed is closely related, after June 10 intelligence development fruit just completely into
Ripe, this result is consistent with the result of example 1.
Embodiment 3:Influence measure of the NaOH solution to seed germination rate and germinating energy
The seed that will be obtained in the intelligence development fruit of collection on June 20, removes flat kind, takes the seed sunk to the bottom, then dry standby
With.In order to optimize the optium concentration and optimal soak time of NaOH solution concentration immersion intelligence development seed, 5 concentration of Setup Experiments
(0.005,0.010,0.015,0.020,0.030g/ml), 4 time gradients are set under each concentration, and (soak time is respectively
12、24、36、48h).The operating procedure of sprouting each handles 3 repetitions, compareed as distilled water immersion 48h with embodiment 2.Point
The germination rate of seed is counted not at 20 days, 25 days, 30 days, 35 days and 40 days.
The influence of table 4.NaOH solution concentrations and soak time to intelligence development seed germination rate
Shown by the data of table 4, under intelligence development seed nature sprouting situation (control treatment), the sprouting cycle length of intelligence development seed,
Sprout to germination rate to tend towards stability since seed and need 20 day time so that growing way is uneven after seed is sprouted, this feature
Very big inconvenience is caused in being produced to actual nursery.
Experiment is found by the NaOH solution and different soak times of various concentrations to intelligence development seed treatment：Low concentration
NaOH solution immersion intelligence development seed can promote seed quick-speed germination, shorten and sprout the cycle, high concentration NaOH solution can suppress to plant
The germination rate of son；When handling 20 days, with the raising of NaOH solution concentration, the germination rate of intelligence development seed shows first to raise to drop afterwards
Low phenomenon, i.e. NaOH solution concentration are in 0.015g/ml, the germination rate highest of intelligence development seed；When handling 30 days, use
0.015g/ml NaOH solution immersion 36h sample seeds germination rate has reached maximum 80.16%, and at control now
It is only 64.35% to manage seed germination rate, and the germination rate of control reaches maximum 75.61% and also needs to 10 day time.Show NaOH
Solution optium concentration is 0.015g/ml, optimal soak time 36h.36h intelligence development is soaked by 0.015g/mlNaOH solution
Seed germination rate improves about 6.25% than control, and it is 10 days to sprout the cycle, though it is shorter 10 days than compareing.Promote seed complete in short time
Sprout in portion so that the seedling growth of sprouting is uniform, is easy to manage.
Claims (4)
1. a kind of method for promoting intelligence development seed to sprout, comprises the steps：
(1) the intelligence development fruit of harvesting ripe：Reach the intelligence development fruit of standard of plucking, cut with scissors together with fruit ear；
(2) seed processing early stage：The fruit ear of step (1) harvesting is stacked in the cool so that Fruit anatomy 5-7 days；It will pass through
Intelligence development fruit after Aging storage, pulp, pericarp, impurity, flat kind are removed, takes full seed, cleaned, shady place is dried, standby；
(3) vernalization：The intelligence development seed dried is immersed in 12-48h in the NaOH solution that concentration is 0.010-0.025g/ml, leaching
It is 28-30 DEG C to steep temperature；
(4) sow：The seed soaked through NaOH solution is rinsed with clear water, is mixed with fine sand and intelligence development seed；By fine sand and seed
Mixture be uniformly sprinkling upon on smooth casting bed, casting bed thickness 5-10cm, then sprinkle the thick decomposed coconut palm chaffs of about 1cm again；With
The uniform spray water of sprayer causes casting bed to keep moistening；Control humiture sprouts seed：30-33 DEG C of daytime, evening temperature
24-26 DEG C, humidity 80%-85%；
In the step (1), the standard for reaching the ripe intelligence development fruit of harvesting is：Single fringe fruit number reaches more than 42；It is single
Fringe fruit fresh weight reaches more than 80g；Simple grain fruit weight reaches more than 1.8g；Dry/fresh weight ratio of fruit is more than 0.34, seed thousand
Grain weighs to more than 11g；TTC methods measure Seed Integrated vigor Index reaches 85%；
In the step (1), reach the selection of intelligence development fruit maturation collecting period：Plucking time is in annual early June to 6 months
Ten days.
2. according to the method for claim 1, it is characterised in that：In the step (3), NaOH solution concentration is 0.015-
0.020g/ml, the soak time in NaOH solution are 12-36h.
3. according to the method for claim 2, it is characterised in that：In the step (3), NaOH solution concentration is 0.015g/
Ml, the soak time in NaOH solution is 36h.
4. according to the method for claim 1, it is characterised in that：In the step (4), clean fine sand and intelligence development seed mix
The part by weight of conjunction is 1:1.
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| CN102640595B (en) * | 2012-04-26 | 2014-03-26 | 辽宁大学 | A method for improving seed germination rate of Zoysia japonica |
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Inventor after: Cheng Hanting Inventor after: Li Guangyi Inventor after: Wang Jinchuang Inventor after: Li Qinfen Inventor after: Hou Xianwen Inventor after: Zou Yukun Inventor before: Cheng Hanting Inventor before: Wang Jinchuang Inventor before: Li Qinfen Inventor before: Hou Xianwen Inventor before: Zou Yukun Inventor before: Li Guangyi |
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