CN105454012A - Method for reducing cadmium content of rice seedling leaves, and rice seedling nutrient solution - Google Patents
Method for reducing cadmium content of rice seedling leaves, and rice seedling nutrient solution Download PDFInfo
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- CN105454012A CN105454012A CN201510916479.8A CN201510916479A CN105454012A CN 105454012 A CN105454012 A CN 105454012A CN 201510916479 A CN201510916479 A CN 201510916479A CN 105454012 A CN105454012 A CN 105454012A
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- rice
- nutrient solution
- rice seedling
- root
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
Abstract
The invention relates to a method for reducing cadmium content of rice seedling leaves, and a rice seedling nutrient solution recipe. The method comprises: (1) rice variety being Yangdao 6; (2) sowing seeds whose surfaces are disinfected to plugs which use vermiculite as medium, putting the plugs in a phytotron to culture for 1-2 weeks; (3) selecting rice seedlings to transplant in cultivating pots, the nutrient solution used for water planting being international rice nutrient solution and being added with microelements. Through discussing influence of external Fe and Mn on formation of rice seedling root iron plaque and on Cd absorption and transport and on rice seedling antioxidant enzyme activities, the method provides new thoughts for rice root iron plaque research and rice safety production on Cd polluted fields.
Description
Technical field
The invention belongs to heavy metal pollution and repair field, relate to a kind of method and the rice seedling culture fluid that reduce Rice Seedling Leaves cadmium content.
Background technology
Large-scale developing and utilizing of mankind's heavy metal mineral resources and fossil fuel, and cause the contents of heavy metal elements such as agricultural land soil Cd to increase fast containing a large amount of uses of heavy metal agricultural chemicals, chemical fertilizer.In soil, Cd has stronger animal migration, and accumulates in human organ easily through food chain, finally works the mischief to health.The whole world about has 3,000,000,000 populations to take paddy rice as staple food, wherein China is paddy rice main product state, also be one of major consumers state of rice, the whole nation is that staple food population ratio reaches more than 60% with rice, and rice field heavy metal Cd pollution amelioration and rice Cd content control to become the large study hotspot of recent domestic one.
Iron film is that water plants conforms and coerces one of important mechanisms.Under flooding condition, Fe a large amount of in rhizosphere soil
2+, Mn
2+to be formed one deck by the dioxygen oxidation secreted from rice root aerenchyma Deng reducing metal element and cover ferric and manganese hydroxide cutan on root surface of rice and root apoplast, i.e. iron film.Ferroelectric oxide film has typical ampholytoid feature, can affect the chemical behavior of metal ion in soil and bio-toxicity by absorption and the physics chemical action such as co-precipitation, thus root border cells to receive with the influence research transporting the heavy metals such as Cd plant absorption and pays close attention to widely.Active oxygen (ROS) level that heavy metal element produces primarily of it injury of plant tissue determines, and Antioxidant Enzyme Systems can remove a large amount of free radical and peroxide that produce in plant corpus under heavy metal stress effectively, in regulation activity oxygen content, play key effect.But add external source Fe and Mn to root border cells formed and to plant growth and physiological effect correlative study few.
Summary of the invention
The object of the invention is to the weak point overcoming existing staple food crop paddy rice heavy metal Cd accumulation control technique, a kind of method and the paddy rice culture fluid formula that reduce paddy rice overground part Cd content are provided, add finite concentration external source Fe and Mn and significantly can increase the formation of root surface of rice iron film; Add external source Fe by significantly reducing Rice Seedling Leaves Cd and ROS content, Cd to be reduced and transport to overground part from root, alleviate rice seedling Cd and poison.
The technical scheme that the present invention realizes object is:
Reduce a method for Rice Seedling Leaves cadmium content,
(1) rice varieties is " raising rice No. 6 ", and the rice paddy seed the selecting full seed NaClO of 3% sterilizes 15min, rear washed with de-ionized water 5 ~ 6 times;
By planting seed after surface sterilization to using vermiculite as the cave of matrix dish in, be placed in phytotron cultivate 1-2 week;
(3) choose uniform four leaf phase rice seedlings to transplant to the black PVC culture tank filling nutrient solution, adaptability carries out test process after cultivating 1-2 week, 2 strain rice seedlings planted by every basin, change weekly nutrient solution once, and water planting nutrient solution trace element formula is as shown in the table:
(4) condition of culture is: day light irradiation time be 14h, night duration 10h, period in daytime temperature is 26 ± 2 DEG C, and period at night temperature is 20 ± 2 DEG C, and humidity is 60%-70%.
And described nutrient solution Fe final concentration is 4mgL
-1; Mn final concentration is 1mgL
-1.
Advantage of the present invention and good effect are:
The present invention adopts interpolation external source Fe that rice seedling root border cells Fe content is significantly increased (114.9%), and Mn and Cd content significantly reduces by 55.2% and 42.4% (p<0.05); Adding external source Mn makes Mn content in iron film significantly increase (180.1%), and meanwhile Cd content significantly reduces by 18.0%.
Adding external source Fe makes rice seedling root Cd content change without significance, and overground part Cd content significantly reduces; Adding external source Mn makes root Cd content significantly increase, and overground part Cd content changes without significance.External source Fe and Mn process make Cd transport coefficient from iron film to root system significantly to raise, and add external source Fe make root system to overground part Cd transport coefficient significantly reduce, simultaneously add Mn make iron film to overground part Cd transport coefficient significantly raise (p<0.05).
Add external source Fe and can significantly reduce ROS content in Rice Seedling Leaves, alleviation Cd coerces generation ROS and poisons rice seedling; And add external source Mn ROS content in blade is significantly increased, GST is active simultaneously significantly increases.
Remarkable increase rice seedling root and the upperground part biomass.
Add finite concentration external source Fe and Mn and significantly can increase the formation of root surface of rice iron film; Add external source Fe by significantly reducing Rice Seedling Leaves Cd and ROS content, Cd to be reduced and transport to overground part from root, alleviate rice seedling Cd and poison.
The present invention is formed rice seedling root border cells by inquiring into external source Fe and Mn and affects Cd absorption and transport and rice seedling activities of antioxidant enzymes, for the safety in production of the research of root surface of rice iron film and Cd polluted agricultural land paddy rice provides new approaches.
Accompanying drawing explanation
Fig. 1 is that different disposal rice seedling root and the upperground part biomass change, and in figure, different lowercase represents significant difference (p<0.05) between reason everywhere;
Fig. 2 is different disposal rice seedling root and overground part Cd changes of contents, and in figure, different lowercase represents significant difference (p<0.05) between reason everywhere.
Embodiment
The inventive method is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention only limited by claims.To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change carry out the material component in these embodiments and consumption or change also belong to protection scope of the present invention.
The present invention adopts water culture experiment, and external source Fe (FeSO is added in research
47H
2and Mn (MnSO O)
4h
2o) rice seedling root border cells is formed, the impact of activities of antioxidant enzymes and Cd absorption and transport.After phytotron cultivates 3 weeks, results sample measures Fe, Mn and Cd content and root and overground part Cd content in root surface of rice iron film respectively, adopts ELISA to measure Rice Seedling Leaves active oxygen (ROS) content and activities of antioxidant enzymes.
Add Fe, Mn trace element and promote that paddy growth suppresses a method for Cd absorption and transport, concrete steps are as follows:
(1) the present invention be " raising rice No. 6 " for examination rice varieties, the rice paddy seed selecting full seed with 3% NaClO sterilization 15min, rear washed with de-ionized water 5 ~ 6 times,
By planting seed after surface sterilization to using vermiculite as the cave of matrix dish in, be placed in phytotron cultivate 2 weeks.
(3) choose uniform four leaf phases rice seedlings transplanting and carry out test process after cultivating 1 week to black PVC culture tank (high 14cm × base diameter 11cm) adaptability filling 1L nutrient solution (1/2 intensity), 2 strain rice seedlings planted by every basin, change weekly nutrient solution once.Test FeSO
47H
2o and MnSO
4h
2the reagent such as O are purchased from Chemical Reagent Co., Ltd., Sinopharm Group.Self-control black PVC culture tank is adopted to cultivate for examination rice seedling.Water planting nutrient solution trace element formula is as shown in the table:
Paddy rice basic culture solution micronutrient levels table
One, test process
Under water planting condition,
(1) add external source Fe (FeSO
47H
2o) water planting liquid Fe final concentration is made to be 4mgL
-1;
(2) add external source Mn (MnSO
4h
2o) water planting liquid Mn final concentration is made to be 1mgL
-1;
(3) without interpolation control group.5 repetitions are established in each process.Above-mentioned process is placed in after phytotron cultivates 3 weeks and receives sample.Condition of culture is: day light irradiation time be 14h, night duration 10h, period in daytime temperature is 26 ± 2 DEG C, and period at night temperature is 20 ± 2 DEG C, and humidity is 60%-70%.
Two, project and method is measured
1, paddy rice ground and underground part Cd assay
By cultivating the rice seedling of 3 weeks under phytotron water planting condition, getting the fresh sample of about 0.2g blade, being transferred to-80 DEG C of refrigerator Cord blood after liquid nitrogen flash freezer stand-by.Along basal part of stem by rice seedling overground part and root system separately, with deionized water rinsing overground part 3 times, root system is soaked into 5mmolL
-115min in EDTA solution, to remove root table attachment Cd, then deionized water rinsing 5 times, respectively overground part and underground part paddy rice sample are loaded paper bag, 75 DEG C complete, and 65 DEG C of oven dry are stand-by to constant weight.Then above-mentioned rice seedling root and overground part cauline leaf are ground respectively, take the sample after about 0.25g mixing, add nitric acid 15ml, shake up standing 6 ~ 8h, then counteract appearance (DigiBlockED54) 80 DEG C of 1.5h of LabTech are positioned over, 120 DEG C of 1.5h, 150 DEG C of 3h, removing 175 DEG C, digestion tube lid catches up with acid to < 1ml, digestion solution high purity water is settled to 25mL, filter rear ICP-MS (iCAP-Qc, GermanThermoFisher) and measure Cd concentration
[11].Blank and standard substance GBW07605 (State center for standard matter) and sample synchronously process, to guarantee the accuracy of mensuration.
2, rice leaf antioxidase measures
Take about 0.2g blade, liquid nitrogen grinding, 5ml Extraction buffer (50mM kaliumphosphate buffer (pH=7) 1mMEDTA, 1%PVPP-30,1mMASC (ascorbic acid) (only adding when APX measures), homogenate 4 DEG C, the centrifugal 10min of 10000r, goes supernatant to be measured.Adopt enzyme-linked immunoassay kit
[12]application double antibody sandwich method measures the SOD (SuperoxideDismutase) in plant, ROS (Reactiveoxygenspecies), MDA (Malondialdehyde), GST (GlutathioneS-transferase), APX (Ascorbateperoxidase).With the phytoenzyme antibody bag of purifying by microwell plate, add enzyme sample, the enzyme antibody marked with HRP (Horseradishperoxidase) is again combined, form antibody-antigene-hrp-antibody complex, substrate TMB (Tetramethylbenzidine) colour developing is added after thorough washing, blueness is changed under the catalysis of HRP enzyme, change into final yellow under the action of an acid, with microplate reader (iMark, BIO-RAD) light absorption value is measured under 450nm wavelength, and by calibration curve calculation sample enzyme concentration.
3, root border cells Fe, Mn and Cd constituent content measures
Root border cells Fe, Mn and Cd content analysis adopts DCB (Dithionite-Citrate-Bicarbonate) extraction method to analyze in conjunction with ICP-MS.After gathering in the crops, rice seedling root system deionized water rinsing 2 times, cuts root from basal part of stem after draining away the water, is placed in and fills 60mLDCB extract (0.03molL
-1trisodium citrate (Na
3c
6h
5o
72H
2and 0.125molL O)
-1sodium bicarbonate (NaHCO
3)) in the beaker of 100mL, take a policy powder (Na simultaneously
2s
2o
4) 1.0g, fully mix, left at room temperature extracts 1h, and then leaching liquor is crossed the pin water system filter membrane of 0.45 μ, 4 DEG C of preservations are to be measured.
4, data analysis
Root surface of rice iron film, root system represent to the ability of overground part transfer Cd with transhipment coefficient (TF).In formula: C
x-Cdrepresent paddy rice DCB, root Cd concentration; C
shoot-Cdrice seedling overground part Cd concentration.Use SPSS16.0 to carry out statistical analysis (SPSS, Inc., Chicago, IL) to data, different disposal data significance take p<0.05 as test stone.
Three, testing result and analysis
1, add external source Fe, Mn to affect rice seedling root border cells metal element content
Compared with control group (CK), add external source Fe and rice seedling root border cells Fe content is significantly increased (114.9%), and Mn and Cd content significantly reduces by 55.2% and 42.4% (p<0.05); Adding external source Mn makes Mn content in iron film significantly increase (180.1%), and meanwhile Cd content significantly reduces by 18.0%, and Fe content is without marked change (table 1).
Tenor in table 1 different disposal iron film
Note: often in row, different lowercase represents significant difference between process (p<0.05).
2, add external source Fe, Mn to affect rice seedling root and the upperground part biomass and Cd content
Compared with control group (CK), interpolation external source Fe, Mn process make rice biological amount significantly increase, root biomass significantly increases 96.5%, 83.0% respectively, and the upperground part biomass significantly increases 43.10%, 29.14% (p<0.05) (Fig. 1) respectively.
Compared with control group (CK), add external source Fe and rice seedling root Cd content is changed without significance, and overground part Cd content significantly reduces by 16.8% (p<0.05); Adding external source Mn makes root Cd content significantly increase 38.4% (p<0.05) and overground part Cd content without significance change (Fig. 2).Cd shows to overground part transhipment result of study at root surface of rice iron film, root, add external source Fe and Mn process to make Cd shift destiny from iron film to root system significantly to raise, and add external source Fe make root system to overground part Cd transport coefficient significantly reduce, simultaneously add Mn make iron film to overground part Cd transport coefficient significantly raise (p<0.05) (table 2).
The coefficient that table 2Cd is transported to root to overground part and iron film by root surface of rice iron film, root
Note: often in row, different lowercase represents significant difference between process (p<0.05)
3, external source Fe, Mn is added on Rice Seedling Leaves ROS and activities of antioxidant enzymes impact
Compared with control group (CK), antioxidase SOD, APX change of interpolation external source Fe, Mn process is little, but ROS is changed significantly, add external source Fe process and significantly reduce 85% in ROS pole compared with the control, but interpolation external source Mn process ROS adds 32% (p<0.05).Add external source Fe, GST is consistent with ROS variation tendency for Mn process antioxidase, add external source Fe process and reduce 12.2% compared with the control, but interpolation external source Mn process significantly increases 8% (p<0.05).
Table 3 different disposal seedling leaves activities of antioxidant enzymes
Note: often in row, different lowercase represents significant difference between process (p<0.05)
Four, result explanation
Compared with control group, add external source Fe and Mn and rice seedling root border cells Fe and Mn constituent content are significantly increased, and Cd content significantly reduces (table 1).External source Fe and Mn by bivalent metal ion adsorption site position in competition root system surface and iron film, can reduce Cd and accumulates in iron film.
Adding external source Fe makes rice seedling overground part Cd content significantly reduce, and adds external source Mn and significantly increase rice seedling root Cd content (Fig. 2).Add external source Fe and Mn process to make Cd shift destiny from iron film to root system significantly to raise, to cause in interpolation external source Mn process more Cd to accumulate in rice seedling root system tissue; And add external source Fe and make root system transport coefficient to overground part Cd significantly to reduce, thus reduce Cd from root toward the transhipment of overground part, contribute to the murder by poisoning reducing Cd top histoorgan over the ground.
Claims (4)
1. reduce a method for Rice Seedling Leaves cadmium content, it is characterized in that:
(1) rice varieties is " raising rice No. 6 ", and the rice paddy seed the selecting full seed NaClO of 3% sterilizes 15min, rear washed with de-ionized water 5 ~ 6 times;
By planting seed after surface sterilization to using vermiculite as the cave of matrix dish in, be placed in phytotron cultivate 1-2 week;
(3) choose uniform four leaf phase rice seedlings to transplant to the black PVC culture tank filling nutrient solution, adaptability carries out test process after cultivating 1-2 week, 2 strain rice seedlings planted by every basin, change weekly nutrient solution once, and water planting nutrient solution trace element formula is as shown in the table:
(4) condition of culture is: day light irradiation time be 14h, night duration 10h, period in daytime temperature is 26 ± 2 DEG C, and period at night temperature is 20 ± 2 DEG C, and humidity is 60%-70%.
2. the method for reduction rice seedling overground part cadmium content according to claim 1, is characterized in that: described nutrient solution Fe final concentration is 4mgL
-1; Mn final concentration is 1mgL
-1.
3. a rice seedling culture fluid, is characterized in that: composition is as follows
Water planting nutrient solution International Rice culture fluid, trace element formula is as shown in the table:
4. rice seedling culture fluid according to claim 3, is characterized in that: described nutrient solution Fe final concentration is 4mgL
-1; Mn final concentration is 1mgL
-1.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107889705A (en) * | 2017-11-23 | 2018-04-10 | 中国科学院南京土壤研究所 | Rice base applies passivator drop living imposed with tillering stage and combining for iron fertilizer root control drops cadmium method |
| CN108157152A (en) * | 2017-12-26 | 2018-06-15 | 云南省农业科学院生物技术与种质资源研究所 | A kind of water-culturing rice method and its application |
| CN108476956A (en) * | 2018-03-30 | 2018-09-04 | 陕西科技大学 | It is a kind of to alleviate application of the cadmium to the ciltivating process and external source molysite of tomato toxic action using external source molysite |
| CN109232083A (en) * | 2018-10-15 | 2019-01-18 | 上海市农业科学院 | A kind of culture solution and its method reducing rice seedling overground part cadmium content |
| CN109287459A (en) * | 2018-09-04 | 2019-02-01 | 东华大学 | A method of inhibit rice absorbing to transport metal nanoparticle |
| CN109566143A (en) * | 2018-12-10 | 2019-04-05 | 佛山科学技术学院 | The water management method of root surface of rice iron film |
| CN110100674A (en) * | 2019-06-24 | 2019-08-09 | 浙江大学 | External source glutamic acid is alleviating rice Cd stress and is reducing the application in rice plant cadmium content |
| CN110771464A (en) * | 2019-12-02 | 2020-02-11 | 湖南省农业环境生态研究所 | Culture solution for reducing rice cadmium accumulation in cadmium-polluted soil and rice planting method |
| CN112317530A (en) * | 2020-09-29 | 2021-02-05 | 海南大学 | Effective method for remedying rice polluted by heavy metal cadmium through fungus organisms |
| CN113105293A (en) * | 2021-04-28 | 2021-07-13 | 中国水稻研究所 | Exogenous salicylic acid-added rice seedling culture solution and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10243746A (en) * | 1997-03-03 | 1998-09-14 | Natl Fedelation Of Agricult Coop Assoc | Production of paddy rice seedling |
| CN101750281A (en) * | 2008-12-05 | 2010-06-23 | 中国科学院沈阳应用生态研究所 | Rice Fe2+ stress spectrum quantification identification method |
| CN102109462A (en) * | 2009-12-23 | 2011-06-29 | 中国科学院沈阳应用生态研究所 | Method for extracting characteristic spectrum information of Cd polluted rice leaf |
| KR20110114353A (en) * | 2010-04-13 | 2011-10-19 | 대한민국(관리부서:농촌진흥청장) | Cultivation method of green-colored tatary buckwehat sprout with high polyphenolic compounds |
| CN102523977A (en) * | 2011-12-09 | 2012-07-04 | 河南科技大学 | Culture method for rice seedlings |
-
2015
- 2015-12-10 CN CN201510916479.8A patent/CN105454012A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10243746A (en) * | 1997-03-03 | 1998-09-14 | Natl Fedelation Of Agricult Coop Assoc | Production of paddy rice seedling |
| CN101750281A (en) * | 2008-12-05 | 2010-06-23 | 中国科学院沈阳应用生态研究所 | Rice Fe2+ stress spectrum quantification identification method |
| CN102109462A (en) * | 2009-12-23 | 2011-06-29 | 中国科学院沈阳应用生态研究所 | Method for extracting characteristic spectrum information of Cd polluted rice leaf |
| KR20110114353A (en) * | 2010-04-13 | 2011-10-19 | 대한민국(관리부서:농촌진흥청장) | Cultivation method of green-colored tatary buckwehat sprout with high polyphenolic compounds |
| CN102523977A (en) * | 2011-12-09 | 2012-07-04 | 河南科技大学 | Culture method for rice seedlings |
Non-Patent Citations (1)
| Title |
|---|
| 候秀等: "铁锰氧化矿物添加对土壤镉有效态及生物效应的影响", 《农业环境科学学报》 * |
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| CN107889705B (en) * | 2017-11-23 | 2020-11-03 | 中国科学院南京土壤研究所 | Combined cadmium reduction method for rice basal application passivant viability reduction and additional iron fertilizer root control in tillering stage |
| CN107889705A (en) * | 2017-11-23 | 2018-04-10 | 中国科学院南京土壤研究所 | Rice base applies passivator drop living imposed with tillering stage and combining for iron fertilizer root control drops cadmium method |
| CN108157152A (en) * | 2017-12-26 | 2018-06-15 | 云南省农业科学院生物技术与种质资源研究所 | A kind of water-culturing rice method and its application |
| CN108157152B (en) * | 2017-12-26 | 2020-11-27 | 云南省农业科学院生物技术与种质资源研究所 | Water culture method for rice and application thereof |
| CN108476956A (en) * | 2018-03-30 | 2018-09-04 | 陕西科技大学 | It is a kind of to alleviate application of the cadmium to the ciltivating process and external source molysite of tomato toxic action using external source molysite |
| CN109287459A (en) * | 2018-09-04 | 2019-02-01 | 东华大学 | A method of inhibit rice absorbing to transport metal nanoparticle |
| CN109232083A (en) * | 2018-10-15 | 2019-01-18 | 上海市农业科学院 | A kind of culture solution and its method reducing rice seedling overground part cadmium content |
| CN109566143A (en) * | 2018-12-10 | 2019-04-05 | 佛山科学技术学院 | The water management method of root surface of rice iron film |
| CN110100674A (en) * | 2019-06-24 | 2019-08-09 | 浙江大学 | External source glutamic acid is alleviating rice Cd stress and is reducing the application in rice plant cadmium content |
| CN110771464A (en) * | 2019-12-02 | 2020-02-11 | 湖南省农业环境生态研究所 | Culture solution for reducing rice cadmium accumulation in cadmium-polluted soil and rice planting method |
| CN112317530A (en) * | 2020-09-29 | 2021-02-05 | 海南大学 | Effective method for remedying rice polluted by heavy metal cadmium through fungus organisms |
| CN113105293A (en) * | 2021-04-28 | 2021-07-13 | 中国水稻研究所 | Exogenous salicylic acid-added rice seedling culture solution and application thereof |
| CN113105293B (en) * | 2021-04-28 | 2022-01-14 | 中国水稻研究所 | Exogenous salicylic acid-added rice seedling culture solution and application thereof |
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