CN109287459A - A method of inhibit rice absorbing to transport metal nanoparticle - Google Patents
A method of inhibit rice absorbing to transport metal nanoparticle Download PDFInfo
- Publication number
- CN109287459A CN109287459A CN201811024461.7A CN201811024461A CN109287459A CN 109287459 A CN109287459 A CN 109287459A CN 201811024461 A CN201811024461 A CN 201811024461A CN 109287459 A CN109287459 A CN 109287459A
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- China
- Prior art keywords
- rice
- metal nanoparticle
- iron film
- transhipment
- rice seedling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides a kind of methods of inhibition rice absorbing transhipment metal nanoparticle, comprising the following steps: step 1: culture rice seedling；Step 2: rice seedling being put into distilled water and is cultivated 12~24 hours；Step 3: preparing iron film and induce liquid, iron film induction liquid pH value is adjusted to 4.5~5.5；Step 4: the rice seedling after step 2 culture being transferred in the iron film induction liquid that step 3 is prepared and is cultivated 24~36 hours；Step 5: iron film is induced after being formed, and rice seedling root distilled water flushing is clean, places into normal nutrition liquid and cultivates 2~3 days；Step 6: by rice seedling transplant to applied or soil that the later period will apply metal nanoparticle correlation agricultural chemicals in.Method provided by the invention can be in the case where ensuring rice normal growth, easy, quickly and efficiently absorption and transport of the reduction rice to metal nanoparticle.
Description
Technical field
The present invention relates to a kind of methods of inhibition rice absorbing transhipment metal nanoparticle, belong to farmland safety production technique
Field.
Background technique
Currently, metal nanoparticle causes people to it in industry, agricultural, medical and commercial field extensive use
The concern of safety problem.Since metal nanoparticle has special physicochemical property, compared with ordinary metallic material, metal nano
Particle can induce higher physiological-toxicity and genetoxic.In fact, a large amount of metal nanoparticle is in its entire Life Cycle
It is interim to be released into environment, potential threat is constituted to human and animal's health.Especially metal nanoparticle can pass through
The application of biosolids or the implementation of environment remediation nanotechnology enter farmland.It is killed moreover, metal nanoparticle is widely used in
In microbial inoculum, Insecticides (tech) & Herbicides (tech) and fertilizer.Therefore, agricultural soil is increasingly becoming the deposition library of metal nanoparticle in the environment.
Element of the plant as the ecosystem, environmental contaminants return become and transport in play crucial work
With.The metal nanoparticle of high concentration can seriously hinder the growth and development of plant, and significantly reduce crop-producing power and nutrition
Quality.Existing research finds that cerium oxide nanoparticles in soil and titania nanoparticles can be respectively by rice, big
The root absorption of beans and cucumber, and it is transferred to aboveground vegetation part, it eventually enters into fruit.However, as people receive metal
The demand of rice grain product is gradually increased with use, can further increase the ambient concentration of metal nanoparticle in soil, finally
Influence agricultural product security.Therefore, need develop a kind of method that can hinder plant that metal nanoparticle is absorbed with transport.
Rice has been used as studying ozone deplation about metal nanoparticle as most staple foods is consumed in the world
Model Plants.Redox Condition and ferrikinetics and Arid Agricultural Tanaka other than plant structure, in The Rhizosphere of Rice waterflooding environment
Wheat be very different.A large amount of specific aerating tissues are formd in rice root and aerial part, which can will be big
Oxygen in gas is transported to root for breathing from leaf.It can from root to the oxygen and other oxidisability exudates of rhizosphere release
With by Fe in flooded soils2+It is oxidized to Fe3+, form root border cells.Root border cells will affect trace metal element and nutrient
Mobility and biological effectiveness.Existing research shows that root border cells turn the absorption of heavy metal ion different in different plants
Fortune influences different.However, influence of the root border cells to plant absorption transport metal nano particle is not reported.
It is a kind of inhibition applicants have found that generating root border cells in metal nanoparticle addition pre-induction rice seedling
The effective ways of rice absorbing transhipment metal nanoparticle.This makes guarantee metal nanoparticle product in the safety of agriculture field
With having a very important significance.
Summary of the invention
The technical problem to be solved by the present invention is how to reduce metal nanoparticle to the negative shadow of plant growth and development
Ring, and reduce metal nanoparticle it is agricultural when be absorbed by plants and be transported in fruit, cause the potential of agricultural product security problem
Risk.
The plant is the wetland plants such as rice.
In order to solve the above-mentioned technical problem, the technical solution of the present invention is to provide a kind of inhibition rice absorbing transhipment metals to receive
The method of rice grain, which comprises the following steps:
Step 1: culture rice seedling；
Step 2: rice seedling being put into distilled water and is cultivated 12~24 hours；
Step 3: preparing iron film and induce liquid, iron film induction liquid pH value is adjusted to 4.5~5.5；
Step 4: by the rice seedling after step 2 culture be transferred to training 24 in the iron film induction liquid that step 3 prepares~
36 hours；
Step 5: iron film is induced after being formed, and rice seedling root distilled water flushing is clean, places into positive nutrient solution
Middle culture 2~3 days；
Step 6: rice seedling is transplanted to having applied or the later period will apply the related agricultural product of metal nanoparticle
Soil in.
Preferably, in the step 1, the somatomedin of rice seedling includes nutrient solution and soil.
Preferably, in the step 1, the somatomedin of rice seedling is rice nutrition liquid.
Preferably, in the step 2, it is small that the rice seedling of 20~22 days seedling ages is put into culture 12~24 in distilled water
When, to reduce the interference that other elements form iron film.
Preferably, in the step 3, Fe2+The common soluble ferrite in plant culture.
It is highly preferred that the soluble ferrite is ferrous sulfate FeSO4·7H2O。
Preferably, in the step 3, it is 10~80mg/LFe that iron film, which induces liquid concentration,2+。
Preferably, in the step 3, it is 40mg/LFe that iron film, which induces liquid concentration,2+。
Preferably, in the step 3, iron film is adjusted with 0.1mol/L NaOH or HCl and induces liquid pH value.
Preferably, in the step 4, rice root needs are protected from light culture；It is protected without being passed through nitrogen into iron film induction liquid
Hold anaerobic environment.
Preferably, in the step 5, with distilled water flushing rice root to remove the remaining Fe of root surface2+；Again by rice
Seedling, which is put into normal nutrition liquid, cultivates 2~3 days to restore rice plant growth's vigor.
Compared with prior art, the invention has the following beneficial effects:
1, the method for the present invention quickly, effectively, can only complete the relevant operation of root border cells induction, and energy within 5 days
Significantly reduce absorption and transhipment of the rice plants to metal nanoparticle；
2, simple, convenient, without customizing dedicated unit；
3, economical and practical, without being passed through nitrogen into medium in root border cells Induction Process；
4, safe and reliable, substantially any negative effect will not be generated to plant growth.
Detailed description of the invention
Fig. 1 is that root border cells induce the shadow for coercing various concentration copper oxide nanometer particle Cu cumulant in lower rice seedling
Ring schematic diagram；(A) influence of rice seedling under ground portion Cu cumulant；(B) influence of rice seedling aerial part Cu cumulant；
Fig. 2 is that root border cells quantity illustrates the 100mg/LCuO NPs influence for coercing Cu cumulant in lower rice seedling
Figure；(A) various concentration Fe2+The root border cells quantity of solution induction；(B) in rice seedling Cu cumulant influence.
Specific embodiment
Present invention will be further explained below with reference to specific examples.
Embodiment 1
The rice seedling for growing consistent 20 days seedling ages is chosen, transfers them in distilled water and cultivates 12 hours.Weigh one
Quantitative FeSO4·7H2O is dissolved in deionized water, prepares 10,20,30,40,80mg/LFe2+Solution.It will be through distilling water process
Rice seedling afterwards is transferred in iron film induction liquid and cultivates 24 hours.Iron film is induced into liquid pH with 0.1mmol/L NaOH or HCl
Value is adjusted to 5.5.Iron film is induced after being formed, and plant deionized water is rinsed 3 times, is placed into aqueous solution 6 hours, to remove
The remaining Fe of root surface2+.It is then placed in normal nutrition liquid and cultivates 24 hours, then rice plant is exposed to containing a series of concentration
Copper oxide nanometer particle (CuO NPs, 0,5,10,50,100mg/L) nutrient solution in grow.Made with unrooted table iron film water rice same
Sample processing compares.
Plant after harvest processing 14 days, thoroughly cleans rice root with distilled water, the iron film on the fresh root system of rice is used
The extraction of dithionite-citrate-bicarbonate (DCB) method.DCB leaching liquor is made of following three kinds of compounds:
0.03M Na3C6H5O7·2H2O and 0.125M NaHCO3Mixed liquor, Na2S2O4Solid.It is soaked using atomic absorption measuring DCB
Fe concentration in extract, quantifies root border cells.Rice after extraction measures Cu cumulant in plant after clearing up.
Result of study shows in no root border cells group, with the increase of CuO NPs concentration, rice seedling root and ground
Cu content in part dramatically increases.However, the Cu content in plant sharply declines by inducing paddy rice root border cells,
Rice root and the Cu content of aerial part reduce 89% and 77% respectively in 100mg/L CuO NPs processing group, such as Fig. 1 institute
Show.It absorbs and transports in rice the result shows that the induction of root border cells significantly reduces CuO NPs.
By induce different number root border cells discovery, foot end Cu content with root border cells quantity increasing
Add and reduce, as shown in Fig. 2, illustrate the increase of root border cells quantity advantageously reduce plant to the absorption of metal nanoparticle with
Transport.Especially work as Fe2+When at concentrations up to 40mg/L, Cu cumulant is minimized in rice root and aerial part.Therefore, iron film lures
Drain concentration is 40mg/LFe2+When, it is best to the inhibitory effect of plant absorption transport metal nano particle.
Embodiment 2
The rice seedling for growing consistent 21 days seedling ages is chosen, transfers them in distilled water and cultivates 18 hours.Weigh one
Quantitative FeSO4·7H2O is dissolved in deionized water, prepares 20mg/LFe2+Solution.It will be through distilled water treated rice seedling
It is transferred in iron film induction liquid and cultivates 30 hours.Iron film induction liquid pH value is adjusted to 5.0 with 0.1mmol/L NaOH or HCl.Iron
Film is induced after being formed, and plant deionized water is rinsed 3 times, is placed into aqueous solution 6 hours, remaining to remove root surface
Fe2+.It is then placed in normal nutrition liquid and cultivates 30 hours, then rice plant is exposed to the NPs nutrient solution of CuO containing 100mg/L
Middle growth.Make same processing with unrooted table iron film water rice to compare.
Plant after harvest processing 14 days, thoroughly cleans rice root with distilled water, the iron film on the fresh root system of rice is used
The extraction of DCB method.DCB leaching liquor is made of following three kinds of compounds: 0.03M Na3C6H5O7·2H2O and 0.125M NaHCO3
Mixed liquor, Na2S2O4Solid.Rice after extraction is after clearing up with Cu cumulant in atomic absorption measuring plant.
After result of study shows that 100mg/L CuO NPs is handled 14 days, in no root border cells group, rice seedling underground part
Divide and the Cu content of aerial part is respectively 9252.17mg/kg and 343.07mg/kg；Have in root border cells group, rice seedling
The Cu content of lower part and aerial part is reduced respectively to 959.15mg/kg and 134.39mg/kg.
Embodiment 3
The rice seedling for growing consistent 22 days seedling ages is chosen, transfers them in distilled water and cultivates 24 hours.Weigh one
Quantitative FeSO4·7H2O is dissolved in deionized water, prepares 20mg/LFe2+Solution.It will be through distilled water treated rice seedling
It is transferred in iron film induction liquid and cultivates 24 hours.Iron film induction liquid pH value is adjusted to 4.5 with 0.1mmol/L NaOH or HCl.Iron
Film is induced after being formed, and plant deionized water is rinsed 3 times, is placed into aqueous solution 6 hours, remaining to remove root surface
Fe2+.It is then placed in normal nutrition liquid and cultivates 36 hours, then rice plant is exposed to the NPs nutrient solution of CuO containing 100mg/L
Middle growth.Make same processing with unrooted table iron film water rice to compare.
Plant after harvest processing 14 days, thoroughly cleans rice root with distilled water, the iron film on the fresh root system of rice is used
The extraction of DCB method.DCB leaching liquor is made of following three kinds of compounds: 0.03M Na3C6H5O7·2H2O and 0.125M NaHCO3
Mixed liquor, Na2S2O4Solid.Rice after extraction is after clearing up with Cu cumulant in atomic absorption measuring plant.
After result of study shows that 100mg/L CuO NPs is handled 14 days, in no root border cells group, rice seedling underground part
Divide and the Cu content of aerial part is respectively 8963.48mg/kg and 321.18mg/kg；Have in root border cells group, rice seedling
The Cu content of lower part and aerial part is reduced respectively to 884.38mg/kg and 108.74mg/kg.
The above, only presently preferred embodiments of the present invention, not to the present invention in any form with substantial limitation,
It should be pointed out that under the premise of not departing from the method for the present invention, can also be made for those skilled in the art
Several improvement and supplement, these are improved and supplement also should be regarded as protection scope of the present invention.All those skilled in the art,
Without departing from the spirit and scope of the present invention, when made using disclosed above technology contents it is a little more
Dynamic, modification and the equivalent variations developed, are equivalent embodiment of the invention；Meanwhile all substantial technologicals pair according to the present invention
The variation, modification and evolution of any equivalent variations made by above-described embodiment, still fall within the range of technical solution of the present invention
It is interior.
Claims (9)
1. a kind of method for inhibiting rice absorbing transhipment metal nanoparticle, which comprises the following steps:
Step 1: culture rice seedling；
Step 2: rice seedling being put into distilled water and is cultivated 12~24 hours；
Step 3: preparing iron film and induce liquid, iron film induction liquid pH value is adjusted to 4.5~5.5；
Step 4: the rice seedling after step 2 culture being transferred in the iron film induction liquid that step 3 is prepared and cultivates 24~36
Hour；
Step 5: iron film is induced after being formed, and rice seedling root distilled water flushing is clean, places into normal nutrition liquid
Culture 2~3 days；
Step 6: rice seedling is transplanted to having applied or the later period will apply metal nanoparticle correlation agricultural chemicals
In soil.
2. a kind of method for inhibiting rice absorbing transhipment metal nanoparticle as described in claim 1, it is characterised in that: described
In step 1, the somatomedin of rice seedling includes nutrient solution and soil.
3. a kind of method for inhibiting rice absorbing transhipment metal nanoparticle as described in claim 1, it is characterised in that: described
In step 2, the rice seedling of 20~22 days seedling ages is put into distilled water and is cultivated 12~24 hours,
To reduce the interference that other elements form iron film.
4. a kind of method for inhibiting rice absorbing transhipment metal nanoparticle as described in claim 1, it is characterised in that: described
In step 3, Fe2+From soluble ferrite.
5. a kind of method for inhibiting rice absorbing transhipment metal nanoparticle as claimed in claim 5, it is characterised in that: described
Soluble ferrite is ferrous sulfate FeSO4·7H2O。
6. a kind of method for inhibiting rice absorbing transhipment metal nanoparticle as described in claim 1, it is characterised in that: described
In step 3, it is 10~80mg/LFe that iron film, which induces liquid concentration,2+。
7. a kind of method for inhibiting rice absorbing transhipment metal nanoparticle as described in claim 1, it is characterised in that: described
In step 3, it is 40mg/LFe that iron film, which induces liquid concentration,2+。
8. a kind of method for inhibiting rice absorbing transhipment metal nanoparticle as described in claim 1, it is characterised in that: described
In step 3, iron film is adjusted with 0.1mol/L NaOH or HCl and induces liquid pH value.
9. a kind of method for inhibiting rice absorbing transhipment metal nanoparticle as described in claim 1, it is characterised in that: described
In step 4, rice root needs are protected from light culture；Anaerobic environment is kept without being passed through nitrogen into iron film induction liquid.
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| CN201811024461.7A CN109287459A (en) | 2018-09-04 | 2018-09-04 | A method of inhibit rice absorbing to transport metal nanoparticle |
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| CN201811024461.7A CN109287459A (en) | 2018-09-04 | 2018-09-04 | A method of inhibit rice absorbing to transport metal nanoparticle |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013035106A1 (en) * | 2011-09-07 | 2013-03-14 | Indo Gulf Fertilizers | A process for manufacturing a composite fertilizer |
| CN103609405A (en) * | 2013-11-21 | 2014-03-05 | 华南农业大学 | Method for covering iron plaques on root surfaces of water planting root system of wetland plants |
| CN104160931A (en) * | 2014-07-08 | 2014-11-26 | 华南农业大学 | Quick induction and identification method for reddish brown iron films on plant root surfaces |
| CN105454012A (en) * | 2015-12-10 | 2016-04-06 | 农业部环境保护科研监测所 | Method for reducing cadmium content of rice seedling leaves, and rice seedling nutrient solution |
-
2018
- 2018-09-04 CN CN201811024461.7A patent/CN109287459A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013035106A1 (en) * | 2011-09-07 | 2013-03-14 | Indo Gulf Fertilizers | A process for manufacturing a composite fertilizer |
| CN103609405A (en) * | 2013-11-21 | 2014-03-05 | 华南农业大学 | Method for covering iron plaques on root surfaces of water planting root system of wetland plants |
| CN104160931A (en) * | 2014-07-08 | 2014-11-26 | 华南农业大学 | Quick induction and identification method for reddish brown iron films on plant root surfaces |
| CN105454012A (en) * | 2015-12-10 | 2016-04-06 | 农业部环境保护科研监测所 | Method for reducing cadmium content of rice seedling leaves, and rice seedling nutrient solution |
Non-Patent Citations (2)
| Title |
|---|
| 刘侯俊等: ""镉处理根表铁膜对水稻吸收镉、锰、铜、锌的影响", 《植物营养与肥料学报》 * |
| 郑芸芸等: "铁膜对水稻根表面电化学性质和氮磷钾短期吸收的影响", 《土壤学报》 * |
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Application publication date: 20190201 |