CN108476956A - It is a kind of to alleviate application of the cadmium to the ciltivating process and external source molysite of tomato toxic action using external source molysite - Google Patents
It is a kind of to alleviate application of the cadmium to the ciltivating process and external source molysite of tomato toxic action using external source molysite Download PDFInfo
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- CN108476956A CN108476956A CN201810276472.8A CN201810276472A CN108476956A CN 108476956 A CN108476956 A CN 108476956A CN 201810276472 A CN201810276472 A CN 201810276472A CN 108476956 A CN108476956 A CN 108476956A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
Alleviating application of the cadmium to the ciltivating process and external source molysite of tomato toxic action using external source molysite the present invention provides a kind of comprising following steps：It after tomato seeds are sterilized with liquor natrii hypochloritis, is uniformly positioned in the pallet equipped with vermiculite, waits for that two leaves wholeheartedly period, a concentration of 200 300 μm of olL are added in its length in Hydroponic Solution‑1Fe solution carry out three weeks by a definite date water planting processing.The present invention effectively reduces the accumulation 46% 62.1% of the Cd in tomato under Cd stress, improves the quality of tomato.
Description
Technical field
The invention belongs to growing vegetables technical fields, and in particular to a kind of application external source molysite, which is alleviated cadmium and poisoned to tomato, to be made
The application of ciltivating process and external source molysite.
Background technology
Cadmium (Cd) is the heavy metal and toxic pollutant of generally existing in environment.Since sewage and waste water is irrigated, chemical fertilizer application
And fast industrialization, cadmium pollution agriculture district area increases and soil cadmium concentration is more and more obvious.
Cadmium has toxicity, mutagenicity and carcinogenicity to most of organisms.Its accumulation in the soil can cause pair
Plant, animal and microorganism toxic action, and human health is influenced by food chain.Cadmium can significantly inhibit soil phosphorus loss, take off
The activity of hydrogen enzyme and urase influences the fauna of edaphon especially bacterium.Soil fertility is caused to decline.Into plant
Cadmium can destroy plant cell membrane permeability, inhibit photosynthesis of plant, respiration, nucleic acid metabolism and enzymatic activity, reduce plant
Yield and quality.
Cd content distributions show the situation gradually risen from northeast to southwest, from northwest to southeastern direction, and Cd points are super
Mark rate is 7.0%, and accounting is maximum in contaminated soil, wherein light contamination, slight pollution, intermediate pollution, serious pollution ratio
Example is respectively 5.2%, 0.8%, 0.5%, 0.5%.The Cd contents of contaminated soil are 2.5~23.0mg/kg, seriously polluted area surface layer
It is even higher that the Cd contents of soil are higher by tens times of subsoil.
Cd is largely enriched in root first after being absorbed by plants in soil, and then a part of Cd by transporting way in vivo
Diameter is migrated to overground part, and in general, distribution trends of the Cd in plant is root ＞ stem ＞ leaf ＞ seeds, accumulation of the root to Cd
The 70%~80% of total amount is accounted for, seed only accounts for 1%~10%, but tobacco and certain vegetables such as carrot blade Cd contents are higher than
Root.
By the Cd of xylem transhipment up to blade murder by poisoning, performance can be generated to blade Determination of Chlorophyll content and photosynthetical system
For chlorophyll content increases with Cd concentration and declines, with symptoms such as apparent chlorisis, yellows.Many studies have shown that heavy metal
The photosynthesis of Stress on Plant has obvious inhibiting effect, and depression effect is positively correlated with processing time and concentration.
Cd mainly enters human body by diet, by blood-transmitted to each organ-tissue of whole body, main accumulation in liver and
In kidney, also there is different degrees of accumulation in organs such as thyroid gland, spleen and pancreases.A large amount of Cd cause acute poisoning symptom after entering human body,
Intense stimulus gastric mucosa can cause vomiting, nausea, diarrhea, abdominal pain.After a large amount of sucking cadmium oxide smog, throat is stimulated, cough occurs
It coughs, is uncomfortable in chest, feeling suffocated, or even showing as having difficulty in breathing, less serious case can cause pulmonary edema, pneumonia, and it is lethal that severe one can suffer from pulmonary heart disease.Low dose
Amount long-term exposure such as long-term drinking Cd
The rice of contaminant water and the edible Cd containing higher concentration, can cause Cd in human body accumulation and induce famous " pain pain
Disease ".In addition, Cd is proven to have carcinogenic, teratogenesis and mutagenic three-induced effect.
Tomato is the vegetable crop planted extensively in world wide, to heavy metal stress rdativery sensitive.The serious shadow of Cd stress
Ring the quality and yield of tomato plant.Ru Shuhua etc. is the study found that tomato is resistant to higher concentration heavy metal, and accumulation ability
It is smaller.Under identical heavy metal pollution degree, accumulated in heavy metal tomato overground part and fruit less.Zhao etc. the study found that
The Cd of low concentration there is facilitation, tomato seedling peroxidase (POD) enzymatic activity to increase Growth of Tomato Seedling, however high
Under concentration C d stress, Growth of Tomato Seedling is suppressed, and POD enzymatic activitys reduce.
Metal ion (Fe (II)) is a kind of well-known nutrient with Cd interactions.Studies have shown that Fe (II)
External source supply increase plant such as arabidopsis and rice can be caused to reduce the uptake of Cd.External source applies Fe alleviation Cd inductions
Arabidopsis leaf chlorosis, growth inhibition and oxidative damage are reduced simultaneously also by the absorption competition of Fe and Cd in arabidopsis
Cd contents.
Have a large amount of report to control by the approach such as liming regulation and control soil pH and regulation and control redox of soil state
Plant Cd accumulation, the essence of these approach are to reduce effective cadmium content of soil.However control Cd accumulation by these approach
While also produce a large amount of inevitable side effects, for example liming improves soil pH, is reducing soil available cadmium
The bio-available Zn concentration of a variety of mineral micronutrient elements such as iron, manganese, copper, zinc etc., unfavorable plant life are also reduced while content
It is long.And fail good solving practical problems in the theoretical supposition stage mostly.
Currently, the research of the heavy metal stress (especially Cd stress) for solving tomato is also relatively fewer, reason is
The mechanism of the heavy metal of influence for to(for) tomato not yet thoroughly research at present, corresponding treating method is still in groping.
Invention content
The technical problem to be solved by the present invention is to：In view of the deficienciess of the prior art, providing a kind of application external source iron
Salt alleviates application of the cadmium to the ciltivating process and external source molysite of tomato toxic action, and the present invention effectively reduces and coerces lower kind in Cd
The accumulation 46%-62.1% of Cd, improves the quality of tomato in eggplant.
To achieve the above object, the present invention uses following technical scheme：
It is a kind of to alleviate cadmium to the ciltivating process of tomato toxic action using external source molysite, include the following steps：
It after tomato seeds are sterilized with liquor natrii hypochloritis, is uniformly positioned in the pallet equipped with vermiculite, waits for its length to two
In leaf wholeheartedly period, a concentration of 200-300 μm of olL is added in Hydroponic Solution-1Fe solution carry out at three weeks water plantings by a definite date
Reason.
The Fe solution is that have by FeSO4And EDTA-Na2By 1：1 (v/v) is formulated, FeSO4A concentration of 0.0278g/
L, EDTA-Na2A concentration of 0.0372g/L.
Preferably, Fe solution is 250 μm of olL-1。
Preferably, the mass concentration of liquor natrii hypochloritis is 3%.
Preferably, water planting condition be illumination in 16 hours, 8 hours dark, 40%-60% relative humidity, 26 ± 2 DEG C of daytime,
20 ± 2 DEG C of night.
A kind of application of external source molysite in the case where promoting Cd stress in tomato growth.
Preferably, using a concentration of 200-300 μm of olL-1Fe solution three weeks by a definite date water plantings are carried out to tomato seedling
Processing.
Preferably, it is after its length to two leaf wholeheartedly period to carry out water planting processing to tomato seedling using Fe solution.
The Fe solution is that have by FeSO4And EDTA-Na2By 1：1 (v/v) is formulated.FeSO4A concentration of 0.0278g/
L, EDTA-Na2A concentration of 0.0372g/L.
Tomato used is Liger (resistance to cadmium) and Tabd (cadmium is sensitive).
Beneficial effects of the present invention：
The present invention is grown in tomato seeds to two leaves wholeheartedly period, and a concentration of 200-300 μm of ol is added in Hydroponic Solution
L-1Fe solution carry out water planting processing in three weeks by a definite date, this method reduce tomato SOD, POD activity and MDA contents, alleviate Cd
The oxidative stress of induction.The present invention improves the lower Contents of Photosynthetic Pigments of Cd stress and photosynthetic rate.Effective reduce is coerced in Cd
The accumulation 46%-62.1% of Cd, improves the quality of tomato in lower tomato；Inhibition of the Cd stress to tomato growth is alleviated, it can
Improve biomass 11.1%-56.5%.
External source molysite is applied in the case where promoting Cd stress in tomato growth, significantly alleviates cadmium to tomato toxic action.It reduces
Tomato SOD, POD activity and MDA contents, alleviate the oxidative stress of Cd inductions, improve the lower Contents of Photosynthetic Pigments of Cd stress and light
Close rate.
Specific implementation mode
The present invention is specifically described below by embodiment, it is necessary to which indicated herein is that following embodiment is only used
It is further detailed in the present invention, should not be understood as limiting the scope of the invention, which is skilled in technique
Personnel still fall within protection scope of the present invention according to some nonessential modifications and adaptations that foregoing invention content is made.
Embodiment 1
Select in the same size, (from the Chinese Academy of Agricultural Sciences, kind is that (cadmium is resistance to by Liger for purchase for the tomato seeds of full seed
By type) and Tabd (cadmium responsive type)), with 3% liquor natrii hypochloritis disinfection 15min, distillation bath flushing 5 times is then used, uniformly
It is positioned in the pallet equipped with vermiculite, keeps adequate water, germinate in 20 DEG C of artificial incubators.To two leaves wholeheartedly period, point
It is 3 groups, 1,2 group of tomato seedling is transferred in the water culture basin containing 1/2Hoagland nutrient solutions, 3 groups of tomato seedlings is shifted
Contain 250 μm of olL to being added-1In the water culture basin of the 1/2Hoagland nutrient solutions of Fe solution, and it is positioned over and automatically controls growth
(illumination 16h, dark 8h, relative humidity 40%-60%, temperature are 26 ± 2 DEG C of (daytime)/20 ± 2 DEG C (night) for culture in room.Culture 3
Zhou Hou selects the consistent strong sprout of growing way and is transplanted, and is cultivated in the 1st group of transplanting to the pancebrin for not adding Cd, as a contrast
(CK), (Cd) is cultivated in second group of transplanting to the pancebrin of addition Cd, a concentration of 5mgL of Cd-1(with CdSO4It is added), third
(Fe) is cultivated in group transplanting to the pancebrin of addition Cd, a concentration of 5mgL of Cd-1(with CdSO4It is added), every group sets 6 weights
It is multiple.
The Tomato Ripening phase samples, and divides root, stem and leaf portion to receive sample, plant sample to be measured crushes respectively, weighing is placed on resolution
Guan Zhong, with 10ml HNO3It impregnates 12 hours, is subsequently placed in digestion furnace at 80 DEG C and clears up 1.5h, clear up 1.5h at 120 DEG C,
Cleared up 3 hours at 150 DEG C, after at 175 DEG C acid adding dissolve.Liquid is transferred in 50ml volumetric flasks, 1%HNO is then used3It is dilute
It releases to graduation mark, with 0.45 μm of membrane filtration liquid, filtrate is spare, and Cd contents are measured with ICP-MS.
Biomass：
It after being cleaned the whole strain of plant sample with tap water, then is rinsed with deionized water, is wiped dry repeatedly.By tomato be divided into root,
Stem and leaf portion weigh fresh weight with electronic balance.It is then dispensed, with baking oven water-removing, drying to constant weight, weighs dry weight.
Activities of antioxidant enzymes：
Superoxide dismutase (SOD), glutathione reductase (GR), the method that catalase (CAT) uses Arora
It measures；Peroxidase (POD), proline (Pro) useMethod measure；Malonaldehyde (MDA) uses
The method of Banerjee measures.
Photosynthetic parameters：
Photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr) 6400 infrared gas analyser (LI- of LI-COR
COR 6400；LI-COR Inc, Lincoln, NE, USA) it measures；Water application efficiency (WUE) is calculated by Pn divided by Tr.It surveys
Amount is in the morning 9:30 and afternoon 4:30 (Beijing times) carried out.
Contents of Photosynthetic Pigments (including chlorophyll a, chlorophyll b, carotenoid)：
Fresh leaf sample is acquired from the tomato plant of three week old, and 0.2g samples is taken to be ground in 80% acetone solns of 5ml,
Homogenate passes through the No.2 filterings of Whatman filter paper.Then use UV/Vis spectrophotometers (Model UV-2102C, UNICO,
USA the absorbance of filtrate) is measured at 470nm, 646nm and 663nm.As a contrast with 80% acetone.
Data processing：
All data are arranged using Origin softwares, statistical analysis using one-way analysis of variance (SPSS Inc,
Chicago, IL, USA), the significant difference between each processing is less than 5%.
External source Fe coerces Cd the influence of Cd accumulation amount in lower tomato
From table 1,2 as can be seen that Cd stress under, the Cd cumulants of two kinds of genotype tomatoes significantly improve, Cd compared with CK,
The Cd accumulations of the type of resistance to cadmium tomato Liger root, stem and leafs have increased separately 185%, 217%, 809%, cadmium responsive type tomato Tabd roots
The Cd accumulations of cauline leaf have increased separately 191%, 153%, 743%, it can be seen that the accumulation of tomato underground part is more compared with overground part
It is more.After Fe salt pre-processes (Fe) compared with unprocessed (Cd), the Cd contents of the rhizome leaf portion of tomato significantly reduce, resistance to cadmium
The Cd accumulations of type tomato Liger root, stem and leafs have dropped 62.0%, 62.1%, 53.7% respectively, cadmium responsive type tomato Tabd roots
The Cd accumulations of cauline leaf have dropped 61.2%, 46.0%, 61.3% respectively, it can be seen that Cd stress under, using external source Fe salt into
After row pretreatment, the cumulant of tomato Cd can be significantly reduced.
Influences of the 1 external source Fe of table to the type of resistance to cadmium tomato (Liger) Cd contents
Influences of the 2 external source Fe of table to cadmium responsive type tomato (Tabd) Cd contents
External source Fe coerces Cd the influence of cadmium in lower two kinds of genotype tomatoes
From table 3,4 as can be seen that under Cd stress, the biomass of two kinds of genotype tomatoes significantly reduces, and Cd is resistance to compared with CK
The Cd accumulations of cadmium type tomato Liger root, stem and leafs reduce 219%, 162%, 125% respectively, the life of cadmium responsive type tomato Tabd
Object amount reduces 282%, 286%, 163% respectively.After Fe salt pre-processes (Fe) compared with unprocessed (Cd), the type of resistance to cadmium
The root of tomato Liger and the biomass of leaf portion dramatically increase, and have increased separately 11.4%, 11.1%, cadmium responsive type tomato
The biomass of Tabd rhizome leaf portions dramatically increases, and has increased separately 56.5%, 28.5%, 37.7%, it can be seen that Cd is coerced
Under, after being pre-processed using external source Fe salt, the biomass of tomato can be dramatically increased, two kinds of genotype tomatoes compare, cadmium
The biomass increase of responsive type tomato Tabd is more notable.
Influences of the 3 external source Fe of table to the type of resistance to cadmium tomato (Liger) biomass
Influences of the 4 external source Fe of table to cadmium responsive type tomato (Tabd) biomass
External source Fe coerces Cd the influence of activities of antioxidant enzymes in lower tomato
From table 5,6 as can be seen that under Cd stress, Cd is compared with CK, and the MDA of the type of resistance to cadmium tomato Liger, CAT, POD are notable
Increase, has increased separately 172%, 175%, 189%, the SOD, MDA, GR of cadmium responsive type tomato Tabd, CAT, POD significantly increase
Add, has increased separately 145%, 87%, 89%, 155%, 100%.Show that the defense reaction of plant is induced to generate.Through Fe salt
(Fe) is pre-processed afterwards compared with unprocessed (Cd), for the activities of antioxidant enzymes of the type of resistance to cadmium tomato Liger without significant change, cadmium is quick
The SOD, GR, CAT of sense type tomato Tabd, POD are significantly reduced, and reduce 61.3%, 82.4%, 74.0%, 70.0% respectively, can
To find out, under Cd stress, murders by poisoning of the Cd to cadmium responsive type tomato Tabd can be alleviated using external source Fe salt.
Influences of the 5 external source Fe of table to the type of resistance to cadmium tomato (Liger) activities of antioxidant enzymes
Influences of the 6 external source Fe of table to cadmium responsive type tomato (Tabd) activities of antioxidant enzymes
From table 7,8 as can be seen that under Cd stress, Cd is compared with CK, the chlorophyll a of the type of resistance to cadmium tomato Liger, chlorophyll b,
Carotenoid content significantly reduces, and reduces 67%, 76%, 27%, but the chlorophyll content of cadmium responsive type tomato Tabd respectively
Significant change is had no, it is smaller to show that Cd stress influences its chlorophyll content.Through Fe salt pre-process (Fe) after with it is unprocessed
(Cd) compare, the chlorophyll a of the type of resistance to cadmium tomato Liger, carotenoid significantly increases, and increases 60.9% respectively,
40.7%, the chlorophyll b of cadmium responsive type tomato Tabd significantly increases, and increases 27.7%, it can be seen that under Cd stress, application
External source Fe salt can alleviate Cd and inhibit to the photosynthesis of the type of resistance to cadmium tomato Liger.
Influences of the 7 external source Fe of table to the type of resistance to cadmium tomato (Liger) photosynthetic pigments
Influences of the 8 external source Fe of table to cadmium responsive type tomato (Tabd) activities of antioxidant enzymes
From table 9,10 as can be seen that under Cd stress, the Net Photosynthetic Rate of the type of resistance to cadmium tomato Liger, stomatal conductance, rising speed
Rate and water use efficiency significantly reduce, and reduce 450%, 600%, 336%, 19% respectively, cadmium responsive type tomato Tabd's
Net Photosynthetic Rate and water use efficiency reduce 46%, 85% respectively.Show in the case where Cd is coerced, the photosynthesis of tomato by
Damage.After Fe salt pre-processes (Fe) compared with unprocessed (Cd), Net Photosynthetic Rate and the moisture profit of the type of resistance to cadmium tomato Liger
171%, 300% has been increased separately with efficiency, has shown that the application of external source Fe salt can mitigate photosynthetic inhibition, cadmium responsive type
Tomato Tabd moisture removal utilization ratios increase 88.2%, and other parameters are all relatively low.
9 external source Fe of table is on the type of resistance to cadmium tomato (Liger) photosynthetic influence
10 external source Fe of table is on cadmium responsive type tomato (Tabd) photosynthetic influence
This experimental studies results show Cd coerce under cause Growth of Tomato Seedling to be restricted, biomass reduce, influence
Photosynthesis etc..External source, which applies Fe, can improve the cadmium toxicity of cadmium patience and cadmium sensitivity tomato dna type, increase the biology of tomato
Amount increases Contents of Photosynthetic Pigments, alleviates photosynthesis performance, reduces Cadmium accumulation and improves the anti-oxidative defense energy of tomato
Power.
Embodiment 2
Select in the same size, (from the Chinese Academy of Agricultural Sciences, kind is that (cadmium is resistance to by Liger for purchase for the tomato seeds of full seed
By type) and Tabd (cadmium responsive type)), with 3% liquor natrii hypochloritis disinfection 15min, distillation bath flushing 5 times is then used, uniformly
It is positioned in the pallet equipped with vermiculite, keeps adequate water, germinate in 20 DEG C of artificial incubators.To two leaves wholeheartedly period, point
It is 3 groups, 1,2 group of tomato seedling is transferred in the water culture basin containing 1/2Hoagland nutrient solutions, 3 groups of tomato seedlings is shifted
Contain 220 μm of olL to being added-1In the water culture basin of the 1/2Hoagland nutrient solutions of Fe solution, and it is positioned over and automatically controls growth
(illumination 16h, dark 8h, relative humidity 40%-60%, temperature are 26 ± 2 DEG C of (daytime)/20 ± 2 DEG C (night) for culture in room.Culture 3
Zhou Hou selects the consistent strong sprout of growing way and is transplanted, and is cultivated in the 1st group of transplanting to the pancebrin for not adding Cd, as a contrast
(CK), (Cd) is cultivated in second group of transplanting to the pancebrin of addition Cd, a concentration of 5mgL of Cd-1(with CdSO4It is added), third
(Fe) is cultivated in group transplanting to the pancebrin of addition Cd, a concentration of 5mgL of Cd-1(with CdSO4It is added), every group sets 6 weights
It is multiple.
The Tomato Ripening phase samples, and divides root, stem and leaf portion to receive sample, plant sample to be measured crushes respectively, weighing is placed on resolution
Guan Zhong, with 10ml HNO3It impregnates 12 hours, is subsequently placed in digestion furnace at 80 DEG C and clears up 1.5h, clear up 1.5h at 120 DEG C,
Cleared up 3 hours at 150 DEG C, after at 175 DEG C acid adding dissolve.Liquid is transferred in 50ml volumetric flasks, 1%HNO is then used3It is dilute
It releases to graduation mark, with 0.45 μm of membrane filtration liquid, filtrate is spare, and Cd contents are measured with ICP-MS.
It finds after testing, under Cd stress, external source, which applies Fe, can significantly reduce the Cd accumulations of tomato, increase tomato
Biomass improves the quality of tomato.
Embodiment 3
Select in the same size, (from the Chinese Academy of Agricultural Sciences, kind is that (cadmium is resistance to by Liger for purchase for the tomato seeds of full seed
By type) and Tabd (cadmium responsive type)), with 3% liquor natrii hypochloritis disinfection 15min, distillation bath flushing 5 times is then used, uniformly
It is positioned in the pallet equipped with vermiculite, keeps adequate water, germinate in 20 DEG C of artificial incubators.To two leaves wholeheartedly period, point
It is 3 groups, 1,2 group of tomato seedling is transferred in the water culture basin containing 1/2Hoagland nutrient solutions, 3 groups of tomato seedlings is shifted
Contain 280 μm of olL to being added-1In the water culture basin of the 1/2Hoagland nutrient solutions of Fe solution, and it is positioned over and automatically controls growth
(illumination 16h, dark 8h, relative humidity 40%-60%, temperature are 26 ± 2 DEG C of (daytime)/20 ± 2 DEG C (night) for culture in room.Culture 3
Zhou Hou selects the consistent strong sprout of growing way and is transplanted, and is cultivated in the 1st group of transplanting to the pancebrin for not adding Cd, as a contrast
(CK), (Cd) is cultivated in second group of transplanting to the pancebrin of addition Cd, a concentration of 5mgL of Cd-1(with CdSO4It is added), third
(Fe) is cultivated in group transplanting to the pancebrin of addition Cd, a concentration of 5mgL of Cd-1(with CdSO4It is added), every group sets 6 weights
It is multiple.
The Tomato Ripening phase samples, and divides root, stem and leaf portion to receive sample, plant sample to be measured crushes respectively, weighing is placed on resolution
Guan Zhong, with 10ml HNO3It impregnates 12 hours, is subsequently placed in digestion furnace at 80 DEG C and clears up 1.5h, clear up 1.5h at 120 DEG C,
Cleared up 3 hours at 150 DEG C, after at 175 DEG C acid adding dissolve.Liquid is transferred in 50ml volumetric flasks, 1%HNO is then used3It is dilute
It releases to graduation mark, with 0.45 μm of membrane filtration liquid, filtrate is spare, and Cd contents are measured with ICP-MS.
It finds after testing, under Cd stress, external source, which applies Fe, can significantly reduce the Cd accumulations of tomato, increase tomato
Biomass improves the quality of tomato, alleviates the oxidative stress of photosynthesis performance and Cd inductions.
Embodiment 4
In addition to Fe solution concentrations are become 300 μm of olL-1, remaining is consistent with embodiment 1.
It finds after testing, under Cd stress, external source, which applies Fe, can significantly reduce the Cd accumulations of tomato, increase tomato
Biomass alleviates photosynthesis performance, improves the quality of tomato.
Embodiment 5
In addition to Fe solution concentrations are become 200 μm of olL-1, remaining is consistent with embodiment 1.
It finds after testing, under Cd stress, external source, which applies Fe, can significantly reduce the Cd accumulations of tomato, improve tomato
Biomass increases Contents of Photosynthetic Pigments, alleviates the oxidative stress of photosynthesis performance and Cd inductions.
Present disclosure is not limited to cited by specific embodiment, and those skilled in the art are right by reading this specification
Any equivalent transformation that technical solution of the present invention is taken is that claim of the invention is covered.
Claims (9)
1. a kind of alleviating ciltivating process of the cadmium to tomato toxic action using external source molysite, which is characterized in that include the following steps：
It after tomato seeds are sterilized with liquor natrii hypochloritis, is uniformly positioned in pallet, waits for its length to two leaves wholeheartedly period, in water
A concentration of 200-300 μm of olL is added in training solution-1Fe solution carry out three weeks by a definite date water planting processing.
2. according to claim 1 alleviate ciltivating process of the cadmium to tomato toxic action using external source molysite, feature exists
In the Fe solution is that have by FeSO4And EDTA-Na2By volume 1：1 is formulated, FeSO4A concentration of 0.0278g/L,
EDTA-Na2A concentration of 0.0372g/L.
3. according to claim 1 alleviate ciltivating process of the cadmium to tomato toxic action using external source molysite, feature exists
In Fe solution is 250 μm of olL-1。
4. according to claim 1 alleviate ciltivating process of the cadmium to tomato toxic action using external source molysite, feature exists
In the mass concentration of liquor natrii hypochloritis is 3%.
5. according to claim 1 alleviate ciltivating process of the cadmium to tomato toxic action using external source molysite, feature exists
In water planting condition is illumination in 16 hours, 8 hours dark, 40%-60% relative humidity, 26 ± 2 DEG C of daytime, 20 ± 2 DEG C of night.
6. a kind of application of external source molysite in the case where promoting Cd stress in tomato growth.
7. application of the external source molysite according to claim 6 in the case where promoting Cd stress in tomato growth, which is characterized in that
Using a concentration of 200-300 μm of olL-1Fe solution to tomato seedling carry out three weeks by a definite date water planting handle.
8. application of the external source molysite according to claim 7 in the case where promoting Cd stress in tomato growth, which is characterized in that adopt
It is after its length to two leaf wholeheartedly period to carry out water planting processing to tomato seedling with Fe solution.
9. application of the external source molysite according to claim 7 in the case where promoting Cd stress in tomato growth, which is characterized in that institute
Stating Fe solution is had by FeSO4And EDTA-Na2By 1：1 volume ratio is formulated, FeSO4A concentration of 0.0278g/L, EDTA-Na2
A concentration of 0.0372g/L.
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| CN112840973A (en) * | 2021-01-15 | 2021-05-28 | 山西农业大学 | Planting method for improving growth and development of tomatoes and fruit quality |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101133710A (en) * | 2007-08-31 | 2008-03-05 | 中国水稻研究所 | Method for controlling the accumulation of the heavy metal cadmium in the rice corn |
| CN105191984A (en) * | 2015-08-17 | 2015-12-30 | 齐鲁工业大学 | Crop protective agent for relieving Cd damage, preparing method thereof and application thereof |
| CN105454012A (en) * | 2015-12-10 | 2016-04-06 | 农业部环境保护科研监测所 | Method for reducing cadmium content of rice seedling leaves, and rice seedling nutrient solution |
| CN105794469A (en) * | 2016-03-31 | 2016-07-27 | 太仓市东泾农场专业合作社 | Method for planting tomatoes rich in microelements |
-
2018
- 2018-03-30 CN CN201810276472.8A patent/CN108476956B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101133710A (en) * | 2007-08-31 | 2008-03-05 | 中国水稻研究所 | Method for controlling the accumulation of the heavy metal cadmium in the rice corn |
| CN105191984A (en) * | 2015-08-17 | 2015-12-30 | 齐鲁工业大学 | Crop protective agent for relieving Cd damage, preparing method thereof and application thereof |
| CN105454012A (en) * | 2015-12-10 | 2016-04-06 | 农业部环境保护科研监测所 | Method for reducing cadmium content of rice seedling leaves, and rice seedling nutrient solution |
| CN105794469A (en) * | 2016-03-31 | 2016-07-27 | 太仓市东泾农场专业合作社 | Method for planting tomatoes rich in microelements |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112840973A (en) * | 2021-01-15 | 2021-05-28 | 山西农业大学 | Planting method for improving growth and development of tomatoes and fruit quality |
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