CN105445485A - Sample suction device and flow fluorescence detector - Google Patents
Sample suction device and flow fluorescence detector Download PDFInfo
- Publication number
- CN105445485A CN105445485A CN201510986751.XA CN201510986751A CN105445485A CN 105445485 A CN105445485 A CN 105445485A CN 201510986751 A CN201510986751 A CN 201510986751A CN 105445485 A CN105445485 A CN 105445485A
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- China
- Prior art keywords
- draw point
- absorbing device
- base plate
- swab
- block
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
Abstract
The invention relates to the technical field of medical equipment, in particular to a sample suction device and a flow fluorescence detector. The sample suction device is used for sucking a sample in a reaction cup and is characterized in that the sample suction device comprises a bottom plate, a stepping motor, a steel needle and a swab, wherein the swab is located right below the lower end of the steel needle, a needle washing passage is arranged in the vertical direction of the swab, the lower end of the steel needle stretches into the needle washing passage, the steel needle is slidingly connected with the bottom plate by a sliding mechanism, the stepping motor drives the steel needle to move up and down, and the steel needle is connected with the sliding mechanism by a switching block. The invention provides the sample suction device which is simple and stable in structure and full-automatic in sample adding and the flow fluorescence detector.
Description
Technical field
The present invention relates to technical field of medical equipment, specifically a kind of sample absorbing device and streaming fluorescence detector.
Background technology
Streaming fluorescence detector take fluorescence-encoded micro-beads as core, the multiple technologies such as collecting type principle, laser analysis, high-speed digital video camera in the multi objective parallel parsing technology platform of one, can once simultaneously accurate quantitative analysis detect 100 kinds of different biomolecule; There is high flux, high sensitivity, the features such as parallel detection; Can be used for the research that immunoassay, nucleic acids research, enzymatic analysis, acceptor, part discriminance analysis etc. are many-sided, multi-field.
The know-why of streaming fluorescence is as follows: unicellular (or particle) suspension after fluorescence labeling is entered suction tube, and then enters flow chamber with sheath fluid.Pipeline before entering flow chamber attenuates, and forces sheath fluid to enter flow chamber from surrounding, sample at center, outside (or from top to bottom) streamlined flow from bottom to top under stressed effect.Sheath fluid is full of flow chamber and is swept along by sample, and when the two is flowed out by flow chamber nozzle, the drop that pressure forces sheath fluid to wrap up comprises single cell or particle perpendicular through detection zone.The position vertical with drop in detection zone arranges laser, arranges detector (lens etc.) in the position with laser vertical, and liquid stream, laser, detector are orthogonal and focus on any and realize Hydrodynamic focus.Fluorescently-labeled cell or particle send the transmitted wave of scattered light and fluorescence under laser excitation, scattered light and utilizing emitted light are obtained by detecting device, remove interference through a series of optical filter, grating process and light signal is inputted computing machine through opto-electronic conversion with after amplifying again, and by software analysis process.Cell sorting is then load plus or minus electric charge respectively to fluorescently-labeled molecules of interest, when its effect being subject to plus high-voltage field in the process of drips deflects and falls into receiving vessel, thus obtains object cell mass.
Existing streaming fluorescence detector is not owing to structure being very stable in the market, cause optical detection unstable, and then testing result is accurate not; In addition, the existing mechanism for inhaling sample is perfect not, there are the factors of instability, inhales the bad control of amount of sample, and needs hand fit's application of sample, therefore, have certain limitation.
Summary of the invention
The invention provides sample absorbing device and the streaming fluorescence detector of simple, stable, the full-automatic application of sample of a kind of structure.
The present invention is achieved through the following technical solutions:
A kind of sample absorbing device, for drawing the sample in reaction cup, comprise base plate, stepper motor, draw point and swab, described swab is positioned at immediately below described draw point lower end, described swab in the vertical direction is provided with washes needle track, the lower end of described draw point stretch into described in wash in needle track, described draw point is slidably connected by slide mechanism and described base plate, described stepper motor drives draw point to move up and down, and described draw point is connected with described slide mechanism by transfer block.
Described slide mechanism comprises guide rail and slide block, described guide rail is vertically located on described base plate, described slide block matches with described guide rail, described transfer block is connected with described slide block, described stepper motor output terminal is provided with driving wheel, described base plate is also provided with engaged wheel, and described engaged wheel is connected with described driving wheel by Timing Belt, and described transfer block is connected with described Timing Belt.
Described sample absorbing device also comprises anticollision mechanism, described anticollision mechanism comprises draw point fixed block, draw point joint, guide pillar, spring and the first optocoupler sensor, described transfer block is provided with a level fluting, described draw point fixed block is located in described level fluting, described draw point upper end is fixed on described draw point fixed block by draw point joint, described guide pillar upper end is fixed in described transfer block, described draw point fixed block is run through in lower end, described draw point fixed block is coaxially provided with shoulder hole with guide pillar, described spring housing is contained on described guide pillar, the lower end of described spring is positioned at described shoulder hole, upper end and described transfer block inconsistent, described draw point fixed block is provided with the first catch, opposite position place in transfer block is located at by described first optocoupler sensor.
One of them side of described swab is provided with washes with described the inlet opening and fluid hole that needle track is connected, and described inlet opening place is provided with liquid inlet joint, and described fluid hole place is provided with fluid joint.
Described transfer block is provided with the second catch, and the opposite position place of described base plate is provided with the second optocoupler sensor, and described second catch passes between described Timing Belt.
Described swab is fixed on described base plate by support plate, and described support plate is L-shaped, and described support plate one end is fixed on described base plate by screw, and the other end is fixed on described swab by screw.
Described engaged wheel is fixed on described base plate by driven wheel carrier.
The upper surface of described transfer block is provided with pressing plate, and described pressing plate is positioned at the top of described draw point joint.
A kind of streaming fluorescence detector, comprises above-mentioned sample absorbing device.
The beneficial effect that the present invention brings is:
In the present invention, described sample absorbing device is full-automatic sample absorbing device, only reaction cup or microwell plate that the sample needing detection is housed need be placed on the below of sample absorbing device, the operation of more than placing can make artificial placement, also can be through certain transmitting device transfers to below sample absorbing device by reaction cup or microwell plate, therefore fully achieve and automatically inhale sample, the stability stablizing and then ensure that optical detection of structure; Slide construction on Xu's idol sample absorbing device can ensure that draw point operates steadily up and down, and described anticollision mechanism ensure that draw point is not easily damaged.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is described in further detail.
Fig. 1 is the structural representation of streaming fluorescence detector of the present invention.
Fig. 2 is the vertical view of streaming fluorescence detector of the present invention.
Fig. 3 is the structural representation of sample absorbing device of the present invention.
Fig. 4 is the sectional view of sample absorbing device of the present invention.
Fig. 5 is the structural representation of the anticollision mechanism of sample absorbing device of the present invention.
Fig. 6 is the partial enlarged drawing at A place in Fig. 5.
Fig. 7 is the structural representation of draw point fixed block of the present invention.
The label that in figure, component names is corresponding is as follows:
1, support body; 2, optical table; 3, the first laser instrument; 4, second laser; 5, flow chamber; 6, flow cell; 7, sample absorbing device; 71, base plate; 72, stepper motor; 73, draw point; 74, swab; 75, needle track is washed; 76, transfer block; 77, guide rail; 78, slide block; 79, driving wheel; 710, engaged wheel; 711, Timing Belt; 712, draw point fixed block; 713, draw point joint; 714, guide pillar; 715, spring; 716, the first optocoupler sensor; 717, level fluting; 718, shoulder hole; 719, the first catch; 720, inlet opening; 721, fluid hole; 722, liquid inlet joint; 723, fluid joint; 724, the second catch; 725, the second optocoupler sensor; 726, support plate; 727, driven wheel carrier; 728, pressing plate; 8, the first optical receiver; 9, the second optical receiver; 10, the 3rd optical receiver; 11, the 4th optical receiver; 12, the first post mirror; 13, the first dichroic mirror; 14, the second post mirror; 15, collimating mirror; 16, the first side mirror; 17, the second side mirror; 18, the second dichroic mirror; 19, damping; 20, beam expanding lens.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in further detail:
As the embodiment of streaming fluorescence detector of the present invention, as depicted in figs. 1 and 2, detect for the sample drawn in reaction cup (for illustrating in figure), described streaming fluorescence detector comprises support body 1, optical table 2, first laser instrument 3, second laser 4, flow chamber 5, flow cell 6, four optical receivers and sample absorbing device 7, described first laser instrument 3, second laser 4, flow chamber 5, flow cell 6 and four optical receivers are all located on optical table 2, described flow chamber 5 is positioned at below flow cell 6, the light that described first laser instrument 3 and second laser 4 produce is received by four optical receivers through flow cell 6, described sample absorbing device 7 is vertically located on support body 1, and be positioned at above reaction cup.
In the present embodiment, described streaming fluorescence detector comprises four optical receivers, be respectively the first optical receiver 8, second optical receiver 9, the 3rd optical receiver 10 and the 4th optical receiver 11, the first post mirror 12, beam expanding lens 20 and the first dichroic mirror 13 is provided with between described first laser instrument 3 and flow cell 6, described first post mirror 12 is located between beam expanding lens 20 and the first dichroic mirror 13, described beam expanding lens 20 is arranged near the first laser instrument 3, is provided with the second post mirror 14 between described first dichroic mirror 13 and second laser 4; Described first optical receiver 8 is positioned at described flow cell 6 rear, between the first optical receiver 8 and flow cell 6, be provided with collimating mirror 15; The left side of described flow cell 6 and right side are respectively equipped with the first side mirror 16 and the second side mirror 17, described second optical receiver 9 is positioned at the rear of the first side mirror 16, described 3rd optical receiver 10 is positioned at the rear of the second side mirror 17, be provided with the second dichroic mirror 18 between described 3rd optical receiver 10 and the second side mirror 17, described 4th optical receiver 11 is positioned at the second dichroic mirror 18 and the described 3rd orthogonal side of optical receiver 10.
As shown in Fig. 3, Fig. 4, Fig. 5, Fig. 6 and Fig. 7, in the present embodiment, described sample absorbing device 7 comprises base plate 71, stepper motor 72, draw point 73 and swab 74, described swab 74 is positioned at immediately below described draw point 73 lower end, described swab 74 in the vertical direction is provided with washes needle track 75, the lower end of described draw point 73 is washed in needle track 75 described in stretching into, described draw point 73 is slidably connected by slide mechanism and described base plate 71, described stepper motor 72 drives draw point 73 to move up and down, and described draw point 73 is connected with described slide mechanism by transfer block 76.
In the present embodiment, described slide mechanism comprises guide rail 77 and slide block 78, described guide rail 77 is vertically located on described base plate 71, described slide block 78 matches with described guide rail 77, described transfer block 76 is connected with described slide block 78, and described stepper motor 72 output terminal is provided with driving wheel 79, and described base plate 71 is also provided with engaged wheel 710, described engaged wheel 710 is connected with described driving wheel 79 by Timing Belt 711, and described transfer block 76 is connected with described Timing Belt 711.
In the present embodiment, described sample absorbing device 7 also comprises anticollision mechanism, described anticollision mechanism comprises draw point fixed block 712, draw point joint 713, guide pillar 714, spring 715 and the first optocoupler sensor 716, described transfer block 76 is provided with a level fluting 717, described draw point fixed block 712 is located in described level fluting 717, described draw point 73 upper end is fixed on described draw point fixed block 712 by draw point joint 713, described guide pillar 714 upper end is fixed in described transfer block 76, described draw point fixed block 712 is run through in lower end, described draw point fixed block 712 is coaxially provided with shoulder hole 718 with guide pillar 714, described spring 715 is sleeved on described guide pillar 714, the lower end of described spring 715 is positioned at described shoulder hole 718, upper end and described transfer block 76 inconsistent, described draw point fixed block 712 is provided with the first catch 719, opposite position place in transfer block 76 is located at by described first optocoupler sensor 716.
In the present embodiment, one of them side of described swab 74 is provided with washes with described the inlet opening 720 and fluid hole 721 that needle track 75 is connected, and described inlet opening 720 place is provided with liquid inlet joint 722, and described fluid hole 721 place is provided with fluid joint 723.
In the present embodiment, described transfer block 76 is provided with the second catch 724, and the opposite position place of described base plate 71 is provided with the second optocoupler sensor 725, and described second catch 724 passes between described Timing Belt 711.
In the present embodiment, described swab 74 is fixed on described base plate 71 by support plate 726, described support plate 726 is L-shaped, and described support plate 726 one end is fixed on described base plate 71 by screw (not shown), and the other end is fixed on described swab 74 by screw (not shown).
In the present embodiment, described engaged wheel 710 is fixed on described base plate 71 by driven wheel carrier 727.
In the present embodiment, the upper surface of described transfer block 76 is provided with pressing plate 728, and described pressing plate 728 is positioned at the top of described draw point joint 713.
Certainly, described streaming fluorescence detector also can comprise two or three or five or six optical receivers.So, also all within scope, repeat no longer one by one here.
Claims (9)
1. a sample absorbing device, for drawing the sample in reaction cup, it is characterized in that comprising base plate, stepper motor, draw point and swab, described swab is positioned at immediately below described draw point lower end, described swab in the vertical direction is provided with washes needle track, the lower end of described draw point stretch into described in wash in needle track, described draw point is slidably connected by slide mechanism and described base plate, described stepper motor drives draw point to move up and down, and described draw point is connected with described slide mechanism by transfer block.
2. sample absorbing device as claimed in claim 1, it is characterized in that described slide mechanism comprises guide rail and slide block, described guide rail is vertically located on described base plate, described slide block matches with described guide rail, described transfer block is connected with described slide block, and described stepper motor output terminal is provided with driving wheel, and described base plate is also provided with engaged wheel, described engaged wheel is connected with described driving wheel by Timing Belt, and described transfer block is connected with described Timing Belt.
3. sample absorbing device as claimed in claim 1, it is characterized in that described sample absorbing device also comprises anticollision mechanism, described anticollision mechanism comprises draw point fixed block, draw point joint, guide pillar, spring and the first optocoupler sensor, described transfer block is provided with a level fluting, described draw point fixed block is located in described level fluting, described draw point upper end is fixed on described draw point fixed block by draw point joint, described guide pillar upper end is fixed in described transfer block, described draw point fixed block is run through in lower end, described draw point fixed block is coaxially provided with shoulder hole with guide pillar, described spring housing is contained on described guide pillar, the lower end of described spring is positioned at described shoulder hole, upper end and described transfer block inconsistent, described draw point fixed block is provided with the first catch, opposite position place in transfer block is located at by described first optocoupler sensor.
4. sample absorbing device as claimed in claim 1, it is characterized in that one of them side of described swab is provided with and wash with described the inlet opening and fluid hole that needle track is connected, described inlet opening place is provided with liquid inlet joint, and described fluid hole place is provided with fluid joint.
5. sample absorbing device as claimed in claim 2, it is characterized in that described transfer block is provided with the second catch, the opposite position place of described base plate is provided with the second optocoupler sensor, and described second catch passes between described Timing Belt.
6. sample absorbing device as claimed in claim 1, it is characterized in that described swab is fixed on described base plate by support plate, described support plate is L-shaped, and described support plate one end is fixed on described base plate by screw, and the other end is fixed on described swab by screw.
7. sample absorbing device as claimed in claim 2, is characterized in that described engaged wheel is fixed on described base plate by driven wheel carrier.
8. sample absorbing device as claimed in claim 3, it is characterized in that the upper surface of described transfer block is provided with pressing plate, described pressing plate is positioned at the top of described draw point joint.
9. a streaming fluorescence detector, is characterized in that comprising the sample absorbing device as described in any one of claim 1-8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510986751.XA CN105445485B (en) | 2015-12-26 | 2015-12-26 | A kind of sample absorbing device and streaming fluorescence detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510986751.XA CN105445485B (en) | 2015-12-26 | 2015-12-26 | A kind of sample absorbing device and streaming fluorescence detector |
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| Publication Number | Publication Date |
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| CN105445485A true CN105445485A (en) | 2016-03-30 |
| CN105445485B CN105445485B (en) | 2017-08-29 |
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| CN201510986751.XA Active CN105445485B (en) | 2015-12-26 | 2015-12-26 | A kind of sample absorbing device and streaming fluorescence detector |
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| CN106770847A (en) * | 2016-12-31 | 2017-05-31 | 浙江福立分析仪器股份有限公司 | Automatic sampler and sample injection method |
| CN108000544A (en) * | 2017-11-06 | 2018-05-08 | 泰州泽成生物技术有限公司 | One kind takes test tube gripper equipment |
| CN108177154A (en) * | 2018-01-26 | 2018-06-19 | 深圳迎凯生物科技有限公司 | Grab cup device |
| CN109030846A (en) * | 2017-06-09 | 2018-12-18 | 深圳迈瑞生物医疗电子股份有限公司 | Sample analyser and its suction needle motion control method |
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| CN201697853U (en) * | 2010-06-09 | 2011-01-05 | 重庆南方数控设备有限责任公司 | Sample injection needle impact protection device for blood viscosity tester |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106770847A (en) * | 2016-12-31 | 2017-05-31 | 浙江福立分析仪器股份有限公司 | Automatic sampler and sample injection method |
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| CN108177154A (en) * | 2018-01-26 | 2018-06-19 | 深圳迎凯生物科技有限公司 | Grab cup device |
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| CN105445485B (en) | 2017-08-29 |
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Denomination of invention: Sample suction device and flow fluorescence detector Effective date of registration: 20200710 Granted publication date: 20170829 Pledgee: Agricultural Bank of China Limited by Share Ltd. Jiaxing science and Technology Branch Pledgor: JIAXING QUEST BIOTECHNOLOGY Co.,Ltd. Registration number: Y2020330000469 |
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Address after: 314000 floor 3, building 1, floor 2-3, building 2, No. 3535, linggongtang Road, Nanhu District, Jiaxing City, Zhejiang Province Patentee after: Jiaxing Kaishi Biotechnology Co.,Ltd. Address before: 314000 east 3 floor 6, 3341 linggong Road, Nanhu District, Jiaxing, Zhejiang. Patentee before: JIAXING QUEST BIOTECHNOLOGY Co.,Ltd. |
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Date of cancellation: 20211126 Granted publication date: 20170829 Pledgee: Agricultural Bank of China Limited by Share Ltd. Jiaxing science and Technology Branch Pledgor: JIAXING QUEST BIOTECHNOLOGY Co.,Ltd. Registration number: Y2020330000469 |