CN205209962U - STREAMING fluorescence detector's liquid way system - Google Patents
STREAMING fluorescence detector's liquid way system Download PDFInfo
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- CN205209962U CN205209962U CN201521094472.4U CN201521094472U CN205209962U CN 205209962 U CN205209962 U CN 205209962U CN 201521094472 U CN201521094472 U CN 201521094472U CN 205209962 U CN205209962 U CN 205209962U
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- solenoid valve
- flow cell
- sheath fluid
- liquid
- fluorescence detector
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Abstract
The utility model belongs to the technical field of the medical equipment technique and specifically relates to a STREAMING fluorescence detector's liquid way system. This STREAMING fluorescence detector's liquid way system includes sample, sheath fluid, swab, sample syringe pump, sheath fluid syringe pump, peristaltic pump and flow cell, be equipped with first solenoid valve and second solenoid valve between flow cell and the sample syringe pump, first solenoid valve is close to the flow cell sets up, the swab up -and -down motion, the lower extreme stretches into appearance is inhaled to the sample, and the upper end is linked together with first solenoid valve, be equipped with the third solenoid valve between sample syringe pump and the sheath fluid, the one end of sheath fluid syringe pump is equipped with the fourth solenoid valve, and the other end is equipped with the 5th solenoid valve, the fourth solenoid valve with the sheath fluid is linked together, the 5th solenoid valve with the flow cell is linked together, the flow cell is linked together through the 6th solenoid valve and useless export, the swab through the 7th solenoid valve with the peristaltic pump is linked together, the peristaltic pump is located between fourth solenoid valve and the 5th solenoid valve.
Description
Technical field
The utility model relates to technical field of medical equipment, specifically a kind of liquid-way system of streaming fluorescence detector.
Background technology
Streaming fluorescence detector take fluorescence-encoded micro-beads as core, the multiple technologies such as collecting type principle, laser analysis, high-speed digital video camera in the multi objective parallel parsing technology platform of one, can once simultaneously accurate quantitative analysis detect 100 kinds of different biomolecule; There is high flux, high sensitivity, the features such as parallel detection; Can be used for the research that immunoassay, nucleic acids research, enzymatic analysis, acceptor, part discriminance analysis etc. are many-sided, multi-field.
The know-why of streaming fluorescence is as follows: unicellular (or particle) suspension after fluorescence labeling is entered suction tube, and then enters flow chamber with sheath fluid.Pipeline before entering flow chamber attenuates, and forces sheath fluid to enter flow chamber from surrounding, sample at center, outside (or from top to bottom) streamlined flow from bottom to top under stressed effect.Sheath fluid is full of flow chamber and is swept along by sample, and when the two is flowed out by flow chamber nozzle, the drop that pressure forces sheath fluid to wrap up comprises single cell or particle perpendicular through detection zone.The position vertical with drop in detection zone arranges laser, arranges detector (lens etc.) in the position with laser vertical, and liquid stream, laser, detector are orthogonal and focus on any and realize Hydrodynamic focus.Fluorescently-labeled cell or particle send the transmitted wave of scattered light and fluorescence under laser excitation, scattered light and utilizing emitted light are obtained by detecting device, remove interference through a series of optical filter, grating process and light signal is inputted computing machine through opto-electronic conversion with after amplifying again, and by software analysis process.Cell sorting is then load plus or minus electric charge respectively to fluorescently-labeled molecules of interest, when its effect being subject to plus high-voltage field in the process of drips deflects and falls into receiving vessel, thus obtains object cell mass.
The road system at night of existing streaming fluorescence detector is all generally arrange peristaltic pump to realize the circulation of various liquid between various parts in the market, peristaltic pump is just as the flexible pipe being full of fluid with finger pinching one, along with finger forward slip tube fluid moves forward, extrude by hocketing to the elasticity delivery hose of pump and discharge pumping fluid, therefore, fluid in pipe can produce a stream of pulses when the running of peristaltic pump, the existence of stream of pulses can make sheath fluid and sample steady not through flow cell, and then affect the effect and accuracy that detect, therefore, there is certain limitation.
Utility model content
The utility model provides the liquid-way system of the stable streaming fluorescence detector of a kind of system stability, feed flow.
The utility model is achieved through the following technical solutions:
A kind of liquid-way system of streaming fluorescence detector, comprise sample, sheath fluid, swab, sample injections pump, sheath fluid syringe pump, peristaltic pump and flow cell, the first solenoid valve and the second solenoid valve is provided with between described flow cell and sample injections pump, described first solenoid valve is arranged near described flow cell, described swab moves up and down, lower end is stretched into described sample and is inhaled sample, upper end is connected with the first solenoid valve, the 3rd solenoid valve is provided with between described sample injections pump and sheath fluid, one end of described sheath fluid syringe pump is provided with the 4th solenoid valve, the other end is provided with the 5th solenoid valve, described 4th solenoid valve is connected with described sheath fluid, described 5th solenoid valve is connected with described flow cell, described flow cell is connected with useless outlet by the 6th solenoid valve, described swab is connected with described peristaltic pump by the 7th solenoid valve, described peristaltic pump is located between described 4th solenoid valve and the 5th solenoid valve.
Vacuum pump is connected with described swab.
Be provided with sheath liquid filter between described flow cell and the 5th solenoid valve, described sheath liquid filter is arranged near described 5th solenoid valve.
Be provided with current limiting tube between described flow cell and the 5th solenoid valve, described current limiting tube is arranged near described flow cell.
Also be provided with pressure transducer between described flow cell and the 5th solenoid valve, described pressure transducer is between described sheath liquid filter and current limiting tube.
Described first solenoid valve, the 4th solenoid valve and the 5th solenoid valve are three-way solenoid valve.
Described second solenoid valve, the 3rd solenoid valve, the 6th solenoid valve and the 7th solenoid valve are two-way electromagnetic valve.
The diameter of described current limiting tube is less than the diameter at its two ends.
Described sample is located in reaction cup or in microwell plate.
Described sheath fluid is located in sheath fluid bottle.
The beneficial effect that the utility model brings is:
In the utility model, the liquid-way system of described streaming fluorescence detector comprises sample, sheath fluid, swab, sample injections pump, sheath fluid syringe pump, peristaltic pump and flow cell, syringe pump is by stepper motor and driver thereof, the formation such as screw mandrel and support, there is the screw mandrel moved back and forth, nut, therefore also referred to as screw mandrel pump, nut is connected with the piston of syringe, liquid is held in syringe, realize high precision, steady pulseless fluid transport, absorption sample and sheath fluid adopt sample injections pump and sheath fluid syringe pump respectively, and then solve the problem that peristaltic pump exists stream of pulses, ensure that sample and the sheath fluid stability when entering flow cell, and then ensure that the accuracy of detection.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the utility model is described in further detail.
Fig. 1 is the structural representation of the liquid-way system of streaming fluorescence detector described in the utility model.
The label that in figure, component names is corresponding is as follows:
1, swab; 2, sample injections pump; 3, sheath fluid syringe pump; 4, peristaltic pump; 5, flow cell; 6, the first solenoid valve; 7, the second solenoid valve; 8, the 3rd solenoid valve; 9, the 4th solenoid valve; 10, the 5th solenoid valve; 11, the 6th solenoid valve; 12, the 7th solenoid valve; 13, vacuum pump; 14, sheath liquid filter; 15, current limiting tube; 16, pressure transducer; 17, reaction cup; 18, sheath fluid bottle.
Embodiment
Below in conjunction with drawings and Examples, the utility model is described in further detail:
As the embodiment of the liquid-way system of streaming fluorescence detector described in the utility model, as shown in Figure 1, comprise sample, sheath fluid, swab 1, sample injections pump 2, sheath fluid syringe pump 3, peristaltic pump 4 and flow cell 5, the first solenoid valve 6 and the second solenoid valve 7 is provided with between described flow cell 5 and sample injections pump 2, described first solenoid valve 6 is arranged near described flow cell 5, described swab 1 moves up and down, lower end is stretched into described sample and is inhaled sample, upper end is connected with the first solenoid valve 6, the 3rd solenoid valve 8 is provided with between described sample injections pump 2 and sheath fluid, one end of described sheath fluid syringe pump 3 is provided with the 4th solenoid valve 9, the other end is provided with the 5th solenoid valve 10, described 4th solenoid valve 9 is connected with described sheath fluid, described 5th solenoid valve 10 is connected with described flow cell 5, described flow cell 5 is connected with useless outlet by the 6th solenoid valve 11, described swab 1 is connected with described peristaltic pump 4 by the 7th solenoid valve 12, described peristaltic pump 4 is located between described 4th solenoid valve 9 and the 5th solenoid valve 10.Absorption sample and sheath fluid adopt sample injections pump 2 and sheath fluid syringe pump 3 respectively, and then solve the problem that original peristaltic pump exists stream of pulses, ensure that sample and the sheath fluid stability when entering flow cell 5, and then ensure that the accuracy of detection.
In the present embodiment, be connected with vacuum pump 13 with described swab 1.Described vacuum pump 13 is for getting rid of the waste liquid in swab 1.
In the present embodiment, be provided with sheath liquid filter 14 between described flow cell 5 and the 5th solenoid valve 10, described sheath liquid filter 14 is arranged near described 5th solenoid valve 10.Described sheath liquid filter 14 has the effect of filtering sheath fluid.
In the present embodiment, be provided with current limiting tube 15 between described flow cell 5 and the 5th solenoid valve 10, described current limiting tube 15 is arranged near described flow cell 5.Described current limiting tube 15 has the effect of current limliting current stabilization, makes the sheath fluid entering flow cell more stable.
In the present embodiment, be also provided with pressure transducer 16 between described flow cell 5 and the 5th solenoid valve 10, described pressure transducer 16 is between described sheath liquid filter 14 and current limiting tube 15.Described pressure transducer 16 is for the pressure in real-time induction tube.
In the present embodiment, described first solenoid valve 6, the 4th solenoid valve 9 and the 5th solenoid valve 10 are three-way solenoid valve.
In the present embodiment, described second solenoid valve 7, the 3rd solenoid valve 8, the 6th solenoid valve 11 and the 7th solenoid valve 12 are two-way electromagnetic valve.
In the present embodiment, the diameter of described current limiting tube 15 is less than the diameter at its two ends.
In the present embodiment, described sample is located in reaction cup 17.
In the present embodiment, described sheath fluid is located in sheath fluid bottle 18.
Certainly, described sample also can be located in microwell plate.So, also all within the utility model protection domain, repeat no longer one by one here.
Claims (10)
1. the liquid-way system of a streaming fluorescence detector, it is characterized in that comprising sample, sheath fluid, swab, sample injections pump, sheath fluid syringe pump, peristaltic pump and flow cell, the first solenoid valve and the second solenoid valve is provided with between described flow cell and sample injections pump, described first solenoid valve is arranged near described flow cell, described swab moves up and down, lower end is stretched into described sample and is inhaled sample, upper end is connected with the first solenoid valve, the 3rd solenoid valve is provided with between described sample injections pump and sheath fluid, one end of described sheath fluid syringe pump is provided with the 4th solenoid valve, the other end is provided with the 5th solenoid valve, described 4th solenoid valve is connected with described sheath fluid, described 5th solenoid valve is connected with described flow cell, described flow cell is connected with useless outlet by the 6th solenoid valve, described swab is connected with described peristaltic pump by the 7th solenoid valve, described peristaltic pump is located between described 4th solenoid valve and the 5th solenoid valve.
2. the liquid-way system of streaming fluorescence detector as claimed in claim 1, is characterized in that being connected with vacuum pump with described swab.
3. the liquid-way system of streaming fluorescence detector as claimed in claim 1, is characterized in that being provided with sheath liquid filter between described flow cell and the 5th solenoid valve, and described sheath liquid filter is arranged near described 5th solenoid valve.
4. the liquid-way system of streaming fluorescence detector as claimed in claim 3, it is characterized in that being provided with current limiting tube between described flow cell and the 5th solenoid valve, described current limiting tube is arranged near described flow cell.
5. the liquid-way system of streaming fluorescence detector as claimed in claim 4, it is characterized in that also being provided with pressure transducer between described flow cell and the 5th solenoid valve, described pressure transducer is between described sheath liquid filter and current limiting tube.
6. the liquid-way system of streaming fluorescence detector as claimed in claim 1, is characterized in that described first solenoid valve, the 4th solenoid valve and the 5th solenoid valve are three-way solenoid valve.
7. the liquid-way system of streaming fluorescence detector as claimed in claim 1, is characterized in that described second solenoid valve, the 3rd solenoid valve, the 6th solenoid valve and the 7th solenoid valve are two-way electromagnetic valve.
8. the liquid-way system of streaming fluorescence detector as claimed in claim 4, is characterized in that the diameter of described current limiting tube is less than the diameter at its two ends.
9. the liquid-way system of streaming fluorescence detector as claimed in claim 1, is characterized in that described sample is located in reaction cup or in microwell plate.
10. the liquid-way system of streaming fluorescence detector as claimed in claim 1, is characterized in that described sheath fluid is located in sheath fluid bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521094472.4U CN205209962U (en) | 2015-12-26 | 2015-12-26 | STREAMING fluorescence detector's liquid way system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521094472.4U CN205209962U (en) | 2015-12-26 | 2015-12-26 | STREAMING fluorescence detector's liquid way system |
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| Publication Number | Publication Date |
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| CN205209962U true CN205209962U (en) | 2016-05-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201521094472.4U Active CN205209962U (en) | 2015-12-26 | 2015-12-26 | STREAMING fluorescence detector's liquid way system |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107012079A (en) * | 2017-04-10 | 2017-08-04 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Digital polymerase chain reaction detection chip, method and its liquid-way system |
| CN107991486A (en) * | 2017-11-17 | 2018-05-04 | 深圳大学 | A kind of liquid-phase chip detection system and method based on quantum dot |
| CN110031386A (en) * | 2019-05-16 | 2019-07-19 | 重庆博奥新景医学科技有限公司 | A kind of flow cytometer liquid channel system and its detection method |
| CN112505013A (en) * | 2020-12-25 | 2021-03-16 | 核工业北京化工冶金研究院 | Aquatic uranium on-line analyzer based on fluorescence method |
| CN112858661A (en) * | 2019-11-28 | 2021-05-28 | 深圳市帝迈生物技术有限公司 | Sample detection system and sample analyzer |
-
2015
- 2015-12-26 CN CN201521094472.4U patent/CN205209962U/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107012079A (en) * | 2017-04-10 | 2017-08-04 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Digital polymerase chain reaction detection chip, method and its liquid-way system |
| CN107991486A (en) * | 2017-11-17 | 2018-05-04 | 深圳大学 | A kind of liquid-phase chip detection system and method based on quantum dot |
| CN110031386A (en) * | 2019-05-16 | 2019-07-19 | 重庆博奥新景医学科技有限公司 | A kind of flow cytometer liquid channel system and its detection method |
| CN110031386B (en) * | 2019-05-16 | 2023-12-26 | 重庆博奥新景医学科技有限公司 | Flow cytometer liquid path system and detection method thereof |
| CN112858661A (en) * | 2019-11-28 | 2021-05-28 | 深圳市帝迈生物技术有限公司 | Sample detection system and sample analyzer |
| CN112505013A (en) * | 2020-12-25 | 2021-03-16 | 核工业北京化工冶金研究院 | Aquatic uranium on-line analyzer based on fluorescence method |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 314000 floor 3, building 1, floor 2-3, building 2, No. 3535, linggongtang Road, Nanhu District, Jiaxing City, Zhejiang Province Patentee after: Jiaxing Kaishi Biotechnology Co.,Ltd. Address before: 314000 east 3 floor 6, 3341 linggong Road, Nanhu District, Jiaxing, Zhejiang. Patentee before: JIAXING QUEST BIOTECHNOLOGY Co.,Ltd. |
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| CP03 | Change of name, title or address |