CN205229051U - STREAMING fluorescence detector - Google Patents
STREAMING fluorescence detector Download PDFInfo
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- CN205229051U CN205229051U CN201521094475.8U CN201521094475U CN205229051U CN 205229051 U CN205229051 U CN 205229051U CN 201521094475 U CN201521094475 U CN 201521094475U CN 205229051 U CN205229051 U CN 205229051U
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- cylindrical lens
- mirror
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- draw point
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Abstract
The utility model belongs to the technical field of the medical equipment technique and specifically relates to a STREAMING fluorescence detector. This STREAMING fluorescence detector is used for absorbing a sample in the reaction cup and goes forward side by side and conduct the survey, and its characterized in that includes support body, optic platform, first laser instrument, second laser instrument, flow chamber, flow cell, a plurality of optical receiver and inhale a kind device, first laser instrument, second laser instrument, flow chamber, flow cell and a plurality of optical receiver are all located on the optic platform, the flow chamber is located the flow cell below, the light that first laser instrument and second laser instrument produced is received by a plurality of optical receivers through the flow cell, inhale that kind device is vertical to be located on the support body to be located reaction cup top. The utility model provides a simple structure, stability, the STREAMING fluorescence detector that detectivity is high, the detection range is wide, optical detection is stable.
Description
Technical field
The utility model relates to technical field of medical equipment, specifically a kind of streaming fluorescence detector.
Background technology
Streaming fluorescence detector take fluorescence-encoded micro-beads as core, the multiple technologies such as collecting type principle, laser analysis, high-speed digital video camera in the multi objective parallel parsing technology platform of one, can once simultaneously accurate quantitative analysis detect 100 kinds of different biomolecule; There is high flux, high sensitivity, the features such as parallel detection; Can be used for the research that immunoassay, nucleic acids research, enzymatic analysis, acceptor, part discriminance analysis etc. are many-sided, multi-field.
The know-why of streaming fluorescence is as follows: unicellular (or particle) suspension after fluorescence labeling is entered suction tube, and then enters flow chamber with sheath fluid.Pipeline before entering flow chamber attenuates, and forces sheath fluid to enter flow chamber from surrounding, sample at center, outside (or from top to bottom) streamlined flow from bottom to top under stressed effect.Sheath fluid is full of flow chamber and is swept along by sample, and when the two is flowed out by flow chamber nozzle, the drop that pressure forces sheath fluid to wrap up comprises single cell or particle perpendicular through detection zone.The position vertical with drop in detection zone arranges laser, arranges detector (lens etc.) in the position with laser vertical, and liquid stream, laser, detector are orthogonal and focus on any and realize Hydrodynamic focus.Fluorescently-labeled cell or particle send the transmitted wave of scattered light and fluorescence under laser excitation, scattered light and utilizing emitted light are obtained by detecting device, remove interference through a series of optical filter, grating process and light signal is inputted computing machine through opto-electronic conversion with after amplifying again, and by software analysis process.Cell sorting is then load plus or minus electric charge respectively to fluorescently-labeled molecules of interest, when its effect being subject to plus high-voltage field in the process of drips deflects and falls into receiving vessel, thus obtains object cell mass.
Existing streaming fluorescence detector is not owing to structure being very stable in the market, cause optical detection unstable, and then testing result is accurate not, therefore, has certain limitation.
Utility model content
The utility model provides the streaming fluorescence detector that a kind of structure is simple, stable, detection sensitivity is high, sensing range is wide, optical detection is stable.
The utility model is achieved through the following technical solutions:
A kind of streaming fluorescence detector, detect for the sample drawn in reaction cup, comprise support body, optical table, the first laser instrument, second laser, flow chamber, flow cell, some optical receivers and sample absorbing device, described first laser instrument, second laser, flow chamber, flow cell and some optical receivers are all located on optical table, described flow chamber is positioned at below flow cell, the light that described first laser instrument and second laser produce is received by some optical receivers through flow cell, described sample absorbing device is vertically located on support body, and is positioned at above reaction cup.
Described sample absorbing device comprises base plate, stepper motor, draw point and swab, described swab is positioned at immediately below described draw point lower end, described swab in the vertical direction is provided with washes needle track, the lower end of described draw point is washed in needle track described in stretching into, described draw point is slidably connected by slide mechanism and described base plate, described stepper motor drives draw point to move up and down, and described draw point is connected with described slide mechanism by transfer block.
Described slide mechanism comprises guide rail and slide block, described guide rail is vertically located on described base plate, described slide block matches with described guide rail, described transfer block is connected with described slide block, described stepper motor output terminal is provided with driving wheel, described base plate is also provided with engaged wheel, and described engaged wheel is connected with described driving wheel by Timing Belt, and described transfer block is connected with described Timing Belt.
Described sample absorbing device also comprises anticollision mechanism, described anticollision mechanism comprises draw point fixed block, draw point joint, guide pillar, spring and the first optocoupler sensor, described transfer block is provided with a level fluting, described draw point fixed block is located in described level fluting, described draw point upper end is fixed on described draw point fixed block by draw point joint, described guide pillar upper end is fixed in described transfer block, described draw point fixed block is run through in lower end, described draw point fixed block is coaxially provided with shoulder hole with guide pillar, described spring housing is contained on described guide pillar, the lower end of described spring is positioned at described shoulder hole, upper end and described transfer block inconsistent, described draw point fixed block is provided with the first catch, opposite position place in transfer block is located at by described first optocoupler sensor.
One of them side of described swab is provided with washes with described the inlet opening and fluid hole that needle track is connected, and described inlet opening place is provided with liquid inlet joint, and described fluid hole place is provided with fluid joint.
Described streaming fluorescence detector comprises four optical receivers, be respectively the first optical receiver, the second optical receiver, the 3rd optical receiver and the 4th optical receiver, the first post mirror and the first dichroic mirror is provided with between described first laser instrument and flow cell, described first post mirror is arranged near the first laser instrument, is provided with the second post mirror between described first dichroic mirror and second laser; Described first optical receiver is positioned at described flow cell rear, between the first optical receiver and flow cell, be provided with collimating mirror; The left side of described flow cell and right side are respectively equipped with the first side mirror and the second side mirror, described second optical receiver is positioned at the rear of the first side mirror, described 3rd optical receiver is positioned at the rear of the second side mirror, be provided with the second dichroic mirror between described 3rd optical receiver and the second side mirror, described 4th optical receiver is positioned at the second dichroic mirror and the described 3rd orthogonal side of optical receiver.
Described first post mirror is identical with the structure of the second post mirror, first post mirror comprises post mirror case, first cylindrical lens, second cylindrical lens and horizontal adjustment platform, the side that described post mirror case two is mutually corresponding is respectively equipped with the first mounting groove and the second mounting groove, be connected by intercommunicating pore between described first mounting groove and the second mounting groove, described first cylindrical lens and the second cylindrical lens are absorbed in described first mounting groove and the second mounting groove respectively by the first post mirror holder and the second post mirror holder, and fixed by the first gland and the second gland, described first cylindrical lens horizontally set, second cylindrical lens is longitudinally arranged, described first gland and the second gland are respectively equipped with the first through hole and the second through hole with described first cylindrical lens and the second cylindrical lens opposite position place, described horizontal adjustment platform is fixed on below post mirror case by card extender, described horizontal adjustment platform is fixed on optical table.
Described first cylindrical lens and be equipped with left and right micro-adjusting mechanism, inside and outside micro-adjusting mechanism and vertical fine adjustment mechanism between the second cylindrical lens and described post mirror case; The left and right micro-adjusting mechanism of described first cylindrical lens comprises Compress Spring and the first holding screw, the left surface of described post mirror case is provided with spring eye, described Compress Spring inserts in described spring eye, the left side of inner and described first cylindrical lens is inconsistent, outer end is conflicted fixing by compressing tablet, described first holding screw compresses described first post mirror holder left from the right flank of post mirror case; The inside and outside micro-adjusting mechanism of described first cylindrical lens comprises two the first bulb plungers, and the both sides being positioned at described intercommunicating pore in described first mounting groove are respectively provided with first mounting hole, and described first bulb plunger is absorbed in described first mounting hole; The vertical fine adjustment mechanism of described first cylindrical lens comprises the second holding screw and two the second bulb plungers, the upper side of described post mirror case is provided with two the second mounting holes, two the second bulb plungers are absorbed in two the second mounting holes respectively, and described second holding screw compresses described first post mirror holder downwards from the upper side of post mirror case; The structure of the left and right micro-adjusting mechanism of the second cylindrical lens is identical with the structure of the vertical fine adjustment mechanism of the first cylindrical lens, the structure of the vertical fine adjustment mechanism of the second cylindrical lens is identical with the structure of the left and right micro-adjusting mechanism of the first cylindrical lens, and the structure of the inside and outside micro-adjusting mechanism of the second cylindrical lens is identical with the structure of the inside and outside micro-adjusting mechanism of the first cylindrical lens.
Described first side mirror is identical with the structure of the second side mirror, described first side mirror comprises side mirror sheet, side mirror frame and three-dimensional adjustment platform, described three-dimensional adjustment platform is fixed on optical table, the top of described three-dimensional adjustment platform is fixed in described side mirror frame one end, described side mirror sheet is located at the other end of described side mirror frame and is positioned at the left side of described flow cell, described side mirror frame is positioned at described side mirror sheet rear and is provided with a light-path, the other end of described light-path is the second optical receiver.
Described 3rd optical receiver is identical with the structure of the 4th optical receiver, described 3rd optical receiver comprises optical filtering, avalanche photodide, contiguous block, screen holder and pcb board, described contiguous block and screen holder are provided with the coaxial optical channel arranged, described optical filtering is located in the optical channel of described screen holder, described avalanche photodide is located in the optical channel of described contiguous block, the rear of described contiguous block is provided with heat-conducting block, described heat-conducting block is provided with refrigerator, radiating block is provided with above described refrigerator, the two ends of described radiating block are fixed on described heat-conducting block respectively by clamping piece, described avalanche photodide is connected with described pcb board, described pcb board is located at the rear of described heat-conducting block, described heat-conducting block is provided with temperature sensor, described temperature sensor is electrically connected with pcb board.
Described optical table is fixed on described support body by some dampings, described damping comprises beam, lower beam, sleeve and vibration-damping screw nail, described optical table is provided with slotted eye, the upper surface of described optical table is provided with a circle depression around described slotted eye, described upper beam is located in described depression, between the lower surface that described optical table is located at by described lower beam and the upper surface of support body, described sleeve is successively through described upper beam, slotted eye and lower beam, the height of described sleeve is less than or equal to lower beam height and optical table height sum, described vibration-damping screw nail is from top to down through described sleeve, and run through described support body, fixed by set nut again.
The beneficial effect that the utility model brings is:
In the utility model, described streaming fluorescence detector has following features:
1. the detection system of high sensitivity, the wide range of linearity, compared with the enzyme linked immunosorbent assay (ELISA) of routine, sensitivity improves 10-100 times, and the range of linearity is high more than 10 times, can reach 3-5 the order of magnitude, and detectable concentration scope is pg-μ g level.
2. can detect 100 kinds of physiopathological index, be qualitative leap at most simultaneously compared with the detection one by one of classic method.
3. the opening of technology and extensibility strong, application relates to clinical and every field that is scientific research.
Described sample absorbing device is full-automatic sample absorbing device, only reaction cup or microwell plate that the sample needing detection is housed need be placed on the below of sample absorbing device, the operation of more than placing can make artificial placement, also can be through certain transmitting device transfers to below sample absorbing device by reaction cup or microwell plate, therefore fully achieve and automatically inhale sample, the stability stablizing and then ensure that optical detection of structure; Slide construction on Xu's idol sample absorbing device can ensure that draw point operates steadily up and down, and described anticollision mechanism ensure that draw point is not easily damaged.
Described side mirror draws together side mirror sheet, side mirror frame and three-dimensional adjustment platform, and described side mirror frame is used for fixation side eyeglass, and described three-dimensional adjustment platform for adjusting the position on the horizontal both direction of side mirror sheet and vertical direction, and then receives better, refracted optical signal.
Described post mirror comprises post mirror case, the first cylindrical lens, the second cylindrical lens and horizontal adjustment platform, according to the feature of cylindrical mirror, a cylinder eyeglass can only regulate the position of single direction, then described first cylindrical lens is arranged with the second cylindrical lens is mutually vertical, the adjustment of two dimensions can be realized, described post mirror case is as the mounting part part of the first cylindrical lens and the second cylindrical lens, described horizontal adjustment platform can adjust the facula position of laser on horizontal level, and then makes optical detection performance more stable, reliable.
Described shock attenuation device comprises beam, lower beam, sleeve and vibration-damping screw nail, described upper beam and lower beam play the effect of damping, described sleeve is on the one hand for limiting upper beam and the upper and lower thickness of lower beam, between described sleeve and vibration-damping screw nail, there is certain gap on the other hand, when there being vibrations, this gap can play certain buffer action.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the utility model is described in further detail.
Fig. 1 is the structural representation of streaming fluorescence detector described in the utility model.
Fig. 2 is the vertical view of streaming fluorescence detector described in the utility model.
Fig. 3 is the structural representation of sample absorbing device described in the utility model.
Fig. 4 is the sectional view of sample absorbing device described in the utility model.
Fig. 5 is the structural representation of the anticollision mechanism of sample absorbing device described in the utility model.
Fig. 6 is the partial enlarged drawing at A place in Fig. 5.
Fig. 7 is the structural representation of draw point fixed block described in the utility model.
Fig. 8 is the structural representation of the first post mirror described in the utility model.
Fig. 9 is the explosive view of the first post mirror described in the utility model.
Figure 10 is the sectional view of the first post mirror described in the utility model.
Figure 11 is the structural representation of the first post mirror holder described in the utility model.
Figure 12 is the structural representation of the first side mirror described in the utility model.
Figure 13 is the sectional view of the first side mirror described in the utility model.
Figure 14 is the structural representation of three-dimensional adjustment platform described in the utility model.
Figure 15 is the structural representation of the 3rd optical receiver described in the utility model.
Figure 16 is the sectional view of the 3rd optical receiver described in the utility model.
Figure 17 is the side view of streaming fluorescence detector described in the utility model.
Figure 18 is the partial enlarged drawing at B place in Figure 17.
Figure 19 is the structural representation of damping optical table described in the utility model.
Figure 20 is the partial enlarged drawing at C place in Figure 19.
The label that in figure, component names is corresponding is as follows:
1, support body; 2, optical table; 3, the first laser instrument; 4, second laser; 5, flow chamber; 6, flow cell; 7, sample absorbing device; 71, base plate; 72, stepper motor; 73, draw point; 74, swab; 75, needle track is washed; 76, transfer block; 77, guide rail; 78, slide block; 79, driving wheel; 710, engaged wheel; 711, Timing Belt; 712, draw point fixed block; 713, draw point joint; 714, guide pillar; 715, spring; 716, the first optocoupler sensor; 717, level fluting; 718, shoulder hole; 719, the first catch; 720, inlet opening; 721, fluid hole; 722, liquid inlet joint; 723, fluid joint; 724, the second catch; 725, the second optocoupler sensor; 726, support plate; 727, driven wheel carrier; 728, pressing plate; 8, the first optical receiver; 9, the second optical receiver; 10, the 3rd optical receiver; 101, optical filtering; 102, avalanche photodide; 103, contiguous block; 104, screen holder; 105, pcb board; 106, optical channel; 107, heat-conducting block; 108, refrigerator; 109, radiating block; 1010, clamping piece; 1011, temperature sensor; 11, the 4th optical receiver; 12, the first post mirror; 121, post mirror case; 122, the first cylindrical lens; 123, the second cylindrical lens; 124, horizontal adjustment platform; 1241, slide; 1242, sliding shoe; 1243, set screw; 125, the first mounting groove; 127, intercommunicating pore; 128, the first post mirror holder; 129, the second post mirror holder; 1210, the first gland; 1211, the second gland; 1212, the first through hole; 1213, the second through hole; 1214, card extender; 1215, Compress Spring; 1216, the first holding screw; 1217, spring eye; 1218, compressing tablet; 1219, the first bulb plunger; 1220, the first mounting hole; 1221, the second holding screw; 1222, the second bulb plunger; 1223, the second mounting hole; 1224, the first light hole; 1225, the first cylindrical lens mounting groove; 1226, arc groove; 1227, linear pattern groove; 1228, chimb; 13, the first dichroic mirror; 14, the second post mirror; 15, collimating mirror; 16, the first side mirror; 161, side mirror sheet; 162, side mirror frame; 163, three-dimensional adjustment platform; 1631, fixed block; 1632, X is to horizontal adjustment block; 1633, Y-direction horizontal adjustment block; 1634, the vertical adjustment block of Z-direction; 1635, X is to adjustment screw; 1636, Y-direction adjustment screw; 164, the first light-path; 165, the second light-path; 166, side mirror sheet mounting groove; 167, arc is awkward-sounding; 17, the second side mirror; 18, the second dichroic mirror; 19, damping; 191, upper beam; 192, lower beam; 193, sleeve; 194, vibration-damping screw nail; 195, slotted eye; 196, cave in; 197, set nut; 198, plain cushion; 20, beam expanding lens.
Embodiment
Below in conjunction with drawings and Examples, the utility model is described in further detail:
As the embodiment of streaming fluorescence detector described in the utility model, as depicted in figs. 1 and 2, detect for the sample drawn in reaction cup (for illustrating in figure), described streaming fluorescence detector comprises support body 1, optical table 2, first laser instrument 3, second laser 4, flow chamber 5, flow cell 6, four optical receivers and sample absorbing device 7, described first laser instrument 3, second laser 4, flow chamber 5, flow cell 6 and four optical receivers are all located on optical table 2, described flow chamber 5 is positioned at below flow cell 6, the light that described first laser instrument 3 and second laser 4 produce is received by four optical receivers through flow cell 6, described sample absorbing device 7 is vertically located on support body 1, and be positioned at above reaction cup.
As shown in Fig. 3, Fig. 4, Fig. 5, Fig. 6 and Fig. 7, in the present embodiment, described sample absorbing device 7 comprises base plate 71, stepper motor 72, draw point 73 and swab 74, described swab 74 is positioned at immediately below described draw point 73 lower end, described swab 74 in the vertical direction is provided with washes needle track 75, the lower end of described draw point 73 is washed in needle track 75 described in stretching into, described draw point 73 is slidably connected by slide mechanism and described base plate 71, described stepper motor 72 drives draw point 73 to move up and down, and described draw point 73 is connected with described slide mechanism by transfer block 76.
In the present embodiment, described slide mechanism comprises guide rail 77 and slide block 78, described guide rail 77 is vertically located on described base plate 71, described slide block 78 matches with described guide rail 77, described transfer block 76 is connected with described slide block 78, and described stepper motor 72 output terminal is provided with driving wheel 79, and described base plate 71 is also provided with engaged wheel 710, described engaged wheel 710 is connected with described driving wheel 79 by Timing Belt 711, and described transfer block 76 is connected with described Timing Belt 711.
In the present embodiment, described sample absorbing device 7 also comprises anticollision mechanism, described anticollision mechanism comprises draw point fixed block 712, draw point joint 713, guide pillar 714, spring 715 and the first optocoupler sensor 716, described transfer block 76 is provided with a level fluting 717, described draw point fixed block 712 is located in described level fluting 717, described draw point 73 upper end is fixed on described draw point fixed block 712 by draw point joint 713, described guide pillar 714 upper end is fixed in described transfer block 76, described draw point fixed block 712 is run through in lower end, described draw point fixed block 712 is coaxially provided with shoulder hole 718 with guide pillar 714, described spring 715 is sleeved on described guide pillar 714, the lower end of described spring 715 is positioned at described shoulder hole 718, upper end and described transfer block 76 inconsistent, described draw point fixed block 712 is provided with the first catch 719, opposite position place in transfer block 76 is located at by described first optocoupler sensor 716.
In the present embodiment, one of them side of described swab 74 is provided with washes with described the inlet opening 720 and fluid hole 721 that needle track 75 is connected, and described inlet opening 720 place is provided with liquid inlet joint 722, and described fluid hole 721 place is provided with fluid joint 723.
In the present embodiment, described transfer block 76 is provided with the second catch 724, and the opposite position place of described base plate 71 is provided with the second optocoupler sensor 725, and described second catch 724 passes between described Timing Belt 711.
In the present embodiment, described swab 74 is fixed on described base plate 71 by support plate 726, described support plate 726 is L-shaped, and described support plate 726 one end is fixed on described base plate 71 by screw (not shown), and the other end is fixed on described swab 74 by screw (not shown).
In the present embodiment, described engaged wheel 710 is fixed on described base plate 71 by driven wheel carrier 727.
In the present embodiment, the upper surface of described transfer block 76 is provided with pressing plate 728, and described pressing plate 728 is positioned at the top of described draw point joint 713.
In the present embodiment, described streaming fluorescence detector comprises four optical receivers, be respectively the first optical receiver 8, second optical receiver 9, the 3rd optical receiver 10 and the 4th optical receiver 11, the first post mirror 12, beam expanding lens 20 and the first dichroic mirror 13 is provided with between described first laser instrument 3 and flow cell 6, described first post mirror 12 is located between beam expanding lens 20 and the first dichroic mirror 13, described beam expanding lens 20 is arranged near the first laser instrument 3, is provided with the second post mirror 14 between described first dichroic mirror 13 and second laser 4; Described first optical receiver 8 is positioned at described flow cell 6 rear, between the first optical receiver 8 and flow cell 6, be provided with collimating mirror 15; The left side of described flow cell 6 and right side are respectively equipped with the first side mirror 16 and the second side mirror 17, described second optical receiver 9 is positioned at the rear of the first side mirror 16, described 3rd optical receiver 10 is positioned at the rear of the second side mirror 17, be provided with the second dichroic mirror 18 between described 3rd optical receiver 10 and the second side mirror 17, described 4th optical receiver 11 is positioned at the second dichroic mirror 18 and the described 3rd orthogonal side of optical receiver 10.
As Fig. 8, Fig. 9, shown in Figure 10 and Figure 11, in the present embodiment, described first post mirror 12 is identical with the structure of the second post mirror 14, first post mirror 12 comprises post mirror case 121, first cylindrical lens 122, second cylindrical lens 123 and horizontal adjustment platform 124, the side that described post mirror case 121 two is mutually corresponding is respectively equipped with the first mounting groove 125 and the second mounting groove (not shown), be connected by intercommunicating pore 127 between described first mounting groove 125 and the second mounting groove, described first cylindrical lens 122 and the second cylindrical lens 123 are absorbed in described first mounting groove 125 and the second mounting groove respectively by the first post mirror holder 128 and the second post mirror holder 129, and fixed by the first gland 1210 and the second gland 1211, described first cylindrical lens 122 horizontally set, second cylindrical lens 123 is longitudinally arranged, described first gland 1210 and the second gland 1211 are respectively equipped with the first through hole 1212 and the second through hole 1213 with described first cylindrical lens 122 and the second cylindrical lens 123 opposite position place, described horizontal adjustment platform 124 is fixed on below post mirror case 121 by card extender 1214, described horizontal adjustment platform 124 is fixed on optical table 2.
In the present embodiment, described first cylindrical lens 122 and be equipped with left and right micro-adjusting mechanism, inside and outside micro-adjusting mechanism and vertical fine adjustment mechanism between the second cylindrical lens 123 and described post mirror case 121; The left and right micro-adjusting mechanism of described first cylindrical lens 122 comprises Compress Spring 1215 and the first holding screw 1216, the left surface of described post mirror case 121 is provided with spring eye 1217, described Compress Spring 1215 inserts in described spring eye 1217, the left side of inner and described first cylindrical lens 122 is inconsistent, outer end is conflicted fixing by compressing tablet 1218, described first holding screw 1216 compresses described first post mirror holder 128 left from the right flank of post mirror case 121; The inside and outside micro-adjusting mechanism of described first cylindrical lens 122 comprises two the first bulb plungers 1219, the both sides being positioned at described intercommunicating pore 127 in described first mounting groove 125 are respectively provided with first mounting hole 1220, and described first bulb plunger 1219 is absorbed in described first mounting hole 1220; The vertical fine adjustment mechanism of described first cylindrical lens 122 comprises the second holding screw 1221 and two the second bulb plungers 1222, the upper side of described post mirror case 121 is provided with two the second mounting holes 1223, two the second bulb plungers 1222 are absorbed in two the second mounting holes 1223 respectively, and described second holding screw 1221 compresses described first post mirror holder 128 downwards from the upper side of post mirror case 121; The structure of the left and right micro-adjusting mechanism of the second cylindrical lens 123 is identical with the structure of the vertical fine adjustment mechanism of the first cylindrical lens 122, the structure of the vertical fine adjustment mechanism of the second cylindrical lens 123 is identical with the structure of the left and right micro-adjusting mechanism of the first cylindrical lens 122, and the structure of the inside and outside micro-adjusting mechanism of the second cylindrical lens 123 is identical with the structure of the inside and outside micro-adjusting mechanism of the first cylindrical lens 122.
In the present embodiment, described horizontal adjustment platform 124 comprises slide 1241 and sliding shoe 1242, described card extender 1214 is located at above described sliding shoe 1242, it is inner that described sliding shoe 1242 is located at described slide 1241 by the form of dovetail groove, and by the position of set screw 1243 adjustable column mirror in the horizontal direction.
In the present embodiment, described first post mirror holder 128 is identical with the structure of the second post mirror holder 129, described first post mirror holder 128 center is provided with the first light hole 1224, be provided with a first cylindrical lens mounting groove 1225 around described first light hole 1224, described first cylindrical lens 122 is absorbed in described first cylindrical lens mounting groove 1225.
In the present embodiment, the sidewall of described first cylindrical lens mounting groove 1225 is provided with two arc grooves 1226, and the degree of depth of described arc groove 1226 is identical with the degree of depth of the first cylindrical lens mounting groove 1225.
In the present embodiment, the leading flank of described first post mirror holder 128 and trailing flank are equipped with straight line type groove 1227, the both sides of described linear pattern groove 1227 are respectively provided with a chimb 1228, and described chimb 1228 is inconsistent with the medial surface of described first gland 1210.
As Figure 12, shown in Figure 13 and Figure 14, in the present embodiment, described first side mirror 16 is identical with the structure of the second side mirror 17, described first side mirror 16 comprises side mirror sheet 161, side mirror frame 162 and three-dimensional adjustment platform 163, described side mirror frame 162 is L-shaped, described three-dimensional adjustment platform 163 is fixed on optical table 2, the top of described three-dimensional adjustment platform 163 is fixed in described side mirror frame 162 one end, described side mirror sheet 161 is located at the other end of described side mirror frame 162 and is positioned at the left side of described flow cell 6, described side mirror frame 162 is positioned at described side mirror sheet 161 rear and is provided with a light-path, the other end of described light-path is the second optical receiver 9.
In the present embodiment, described three-dimensional adjustment platform 163 comprises fixed block 1631, X to horizontal adjustment block 1632, Y-direction horizontal adjustment block 1633 and the vertical adjustment block 1634 of Z-direction, described Y-direction horizontal adjustment block 1633 is located at described X between horizontal adjustment block 1632 and the vertical adjustment block 1634 of Z-direction, and described fixed block 1631 is located at described X below horizontal adjustment block 1632.
In the present embodiment, described light-path is made up of the first light-path of two sections of different-diameters and 164 second light-pathes 165, and the diameter of described first light-path 164 is less than the diameter of the second light-path 165.
In the present embodiment, one end and the described light-path of described side mirror frame 162 fixation side eyeglass 161 are coaxially arranged with a side mirror sheet mounting groove 166, described side mirror sheet 161 is absorbed in described side mirror sheet mounting groove 166, the diameter of described side mirror sheet mounting groove 166 is greater than the diameter of side mirror sheet 161, and the diameter of described side mirror sheet 161 is greater than the diameter of the first light-path 164.
In the present embodiment, the lateral edges place of described side mirror sheet mounting groove 166 is provided with an arc awkward-sounding 167.
In the present embodiment, described X slides in the horizontal direction to the form by dovetail groove between horizontal adjustment block 1632 and fixed block 1631, and described Y-direction adjustment block 1633 and X are slided in the horizontal direction by the form of dovetail groove between horizontal adjustment block 1632.
In the present embodiment, described X is slided to adjustment screw 1635 to horizontal adjustment block 1632 in the horizontal direction by X, and Y-direction horizontal adjustment block 1633 is slided in the horizontal direction by Y-direction adjustment screw 1636.
As shown in Figure 15 and Figure 16, in the present embodiment, described 3rd optical receiver 10 is identical with the structure of the 4th optical receiver 11, described 3rd optical receiver 10 comprises optical filtering 101, avalanche photodide 102, contiguous block 103, screen holder 104 and pcb board 105, described contiguous block 103 and screen holder 104 are provided with the coaxial optical channel 106 arranged, described optical filtering 101 is located in the optical channel 106 of described screen holder 104, described avalanche photodide 102 is located in the optical channel 106 of described contiguous block 103, the rear of described contiguous block 103 is provided with heat-conducting block 107, described heat-conducting block 107 is provided with refrigerator 108, radiating block 109 is provided with above described refrigerator 108, the two ends of described radiating block 109 are fixed on described heat-conducting block 107 respectively by clamping piece 1010, described avalanche photodide 102 is connected with described pcb board 105, described pcb board 105 is located at the rear of described heat-conducting block 107, described heat-conducting block 107 is provided with temperature sensor 1011, described temperature sensor 1011 is electrically connected with pcb board 105.
In the present embodiment, described optical filtering 101 is fixed in the optical channel 106 on described screen holder 104 by rotary gland 1012.
In the present embodiment, the light signal receiving-member in described first optical receiver 8 is photodiode, and the light signal receiving-member in the second optical receiver 9 is photomultiplier.
As Figure 17, Figure 18, shown in Figure 19 and Figure 20, in the present embodiment, described optical table 2 is fixed on described support body 1 by four dampings 19, described damping 19 comprises beam 191, lower beam 192, sleeve 193 and vibration-damping screw nail 194, described optical table 2 is provided with slotted eye 195, the upper surface of described optical table 2 is provided with a circle depression 196 around described slotted eye 195, described upper beam 191 is located in described depression 196, between the lower surface that described optical table 2 is located at by described lower beam 192 and the upper surface of support body 1, described sleeve 193 is successively through described upper beam 191, slotted eye 195 and lower beam 192, the height of described sleeve 193 equals lower beam 192 height and optical table 2 height sum, described vibration-damping screw nail 194 is from top to down through described sleeve 193, and run through described support body 1, fixed by set nut 197 again, the lower surface of described sleeve 193 and the upper surface of described support body 1 inconsistent.
In the present embodiment, the diameter of described sleeve 193 is D1, and the external diameter of described slotted eye 195 is D2, and the diameter of described upper beam 191 is D3, and the diameter of described depression 196 is D4, then, and and D1 ﹤ D2 ﹤ D3 ﹤ D4.
In the present embodiment, the top of described upper beam 191 is provided with plain cushion 198, and described vibration-damping screw nail 194 is through described plain cushion 198.
Certainly, described streaming fluorescence detector also can comprise two or three or five or six optical receivers; Described optical table also can be fixed on described support body by three or five or six dampings.So, also all within the utility model protection domain, repeat no longer one by one here.
Claims (11)
1. a streaming fluorescence detector, detect for the sample drawn in reaction cup, it is characterized in that comprising support body, optical table, first laser instrument, second laser, flow chamber, flow cell, some optical receivers and sample absorbing device, described first laser instrument, second laser, flow chamber, flow cell and some optical receivers are all located on optical table, described flow chamber is positioned at below flow cell, the light that described first laser instrument and second laser produce is received by some optical receivers through flow cell, described sample absorbing device is vertically located on support body, and be positioned at above reaction cup.
2. streaming fluorescence detector as claimed in claim 1, it is characterized in that described sample absorbing device comprises base plate, stepper motor, draw point and swab, described swab is positioned at immediately below described draw point lower end, described swab in the vertical direction is provided with washes needle track, the lower end of described draw point is washed in needle track described in stretching into, described draw point is slidably connected by slide mechanism and described base plate, and described stepper motor drives draw point to move up and down, and described draw point is connected with described slide mechanism by transfer block.
3. streaming fluorescence detector as claimed in claim 2, it is characterized in that described slide mechanism comprises guide rail and slide block, described guide rail is vertically located on described base plate, described slide block matches with described guide rail, described transfer block is connected with described slide block, and described stepper motor output terminal is provided with driving wheel, and described base plate is also provided with engaged wheel, described engaged wheel is connected with described driving wheel by Timing Belt, and described transfer block is connected with described Timing Belt.
4. streaming fluorescence detector as claimed in claim 2, it is characterized in that described sample absorbing device also comprises anticollision mechanism, described anticollision mechanism comprises draw point fixed block, draw point joint, guide pillar, spring and the first optocoupler sensor, described transfer block is provided with a level fluting, described draw point fixed block is located in described level fluting, described draw point upper end is fixed on described draw point fixed block by draw point joint, described guide pillar upper end is fixed in described transfer block, described draw point fixed block is run through in lower end, described draw point fixed block is coaxially provided with shoulder hole with guide pillar, described spring housing is contained on described guide pillar, the lower end of described spring is positioned at described shoulder hole, upper end and described transfer block inconsistent, described draw point fixed block is provided with the first catch, opposite position place in transfer block is located at by described first optocoupler sensor.
5. streaming fluorescence detector as claimed in claim 2, it is characterized in that one of them side of described swab is provided with and wash with described the inlet opening and fluid hole that needle track is connected, described inlet opening place is provided with liquid inlet joint, and described fluid hole place is provided with fluid joint.
6. streaming fluorescence detector as claimed in claim 1, it is characterized in that described streaming fluorescence detector comprises four optical receivers, be respectively the first optical receiver, the second optical receiver, the 3rd optical receiver and the 4th optical receiver, the first post mirror and the first dichroic mirror is provided with between described first laser instrument and flow cell, described first post mirror is arranged near the first laser instrument, is provided with the second post mirror between described first dichroic mirror and second laser; Described first optical receiver is positioned at described flow cell rear, between the first optical receiver and flow cell, be provided with collimating mirror; The left side of described flow cell and right side are respectively equipped with the first side mirror and the second side mirror, described second optical receiver is positioned at the rear of the first side mirror, described 3rd optical receiver is positioned at the rear of the second side mirror, be provided with the second dichroic mirror between described 3rd optical receiver and the second side mirror, described 4th optical receiver is positioned at the second dichroic mirror and the described 3rd orthogonal side of optical receiver.
7. streaming fluorescence detector as claimed in claim 6, it is characterized in that described first post mirror is identical with the structure of the second post mirror, first post mirror comprises post mirror case, first cylindrical lens, second cylindrical lens and horizontal adjustment platform, the side that described post mirror case two is mutually corresponding is respectively equipped with the first mounting groove and the second mounting groove, be connected by intercommunicating pore between described first mounting groove and the second mounting groove, described first cylindrical lens and the second cylindrical lens are absorbed in described first mounting groove and the second mounting groove respectively by the first post mirror holder and the second post mirror holder, and fixed by the first gland and the second gland, described first cylindrical lens horizontally set, second cylindrical lens is longitudinally arranged, described first gland and the second gland are respectively equipped with the first through hole and the second through hole with described first cylindrical lens and the second cylindrical lens opposite position place, described horizontal adjustment platform is fixed on below post mirror case by card extender, described horizontal adjustment platform is fixed on optical table.
8. streaming fluorescence detector as claimed in claim 7, is characterized in that described first cylindrical lens and is equipped with left and right micro-adjusting mechanism, inside and outside micro-adjusting mechanism and vertical fine adjustment mechanism between the second cylindrical lens and described post mirror case; The left and right micro-adjusting mechanism of described first cylindrical lens comprises Compress Spring and the first holding screw, the left surface of described post mirror case is provided with spring eye, described Compress Spring inserts in described spring eye, the left side of inner and described first cylindrical lens is inconsistent, outer end is conflicted fixing by compressing tablet, described first holding screw compresses described first post mirror holder left from the right flank of post mirror case; The inside and outside micro-adjusting mechanism of described first cylindrical lens comprises two the first bulb plungers, and the both sides being positioned at described intercommunicating pore in described first mounting groove are respectively provided with first mounting hole, and described first bulb plunger is absorbed in described first mounting hole; The vertical fine adjustment mechanism of described first cylindrical lens comprises the second holding screw and two the second bulb plungers, the upper side of described post mirror case is provided with two the second mounting holes, two the second bulb plungers are absorbed in two the second mounting holes respectively, and described second holding screw compresses described first post mirror holder downwards from the upper side of post mirror case; The structure of the left and right micro-adjusting mechanism of the second cylindrical lens is identical with the structure of the vertical fine adjustment mechanism of the first cylindrical lens, the structure of the vertical fine adjustment mechanism of the second cylindrical lens is identical with the structure of the left and right micro-adjusting mechanism of the first cylindrical lens, and the structure of the inside and outside micro-adjusting mechanism of the second cylindrical lens is identical with the structure of the inside and outside micro-adjusting mechanism of the first cylindrical lens.
9. streaming fluorescence detector as claimed in claim 6, it is characterized in that described first side mirror is identical with the structure of the second side mirror, described first side mirror comprises side mirror sheet, side mirror frame and three-dimensional adjustment platform, described three-dimensional adjustment platform is fixed on optical table, the top of described three-dimensional adjustment platform is fixed in described side mirror frame one end, described side mirror sheet is located at the other end of described side mirror frame and is positioned at the left side of described flow cell, described side mirror frame is positioned at described side mirror sheet rear and is provided with a light-path, the other end of described light-path is the second optical receiver.
10. streaming fluorescence detector as claimed in claim 6, it is characterized in that described 3rd optical receiver is identical with the structure of the 4th optical receiver, described 3rd optical receiver comprises optical filtering, avalanche photodide, contiguous block, screen holder and pcb board, described contiguous block and screen holder are provided with the coaxial optical channel arranged, described optical filtering is located in the optical channel of described screen holder, described avalanche photodide is located in the optical channel of described contiguous block, the rear of described contiguous block is provided with heat-conducting block, described heat-conducting block is provided with refrigerator, radiating block is provided with above described refrigerator, the two ends of described radiating block are fixed on described heat-conducting block respectively by clamping piece, described avalanche photodide is connected with described pcb board, described pcb board is located at the rear of described heat-conducting block, described heat-conducting block is provided with temperature sensor, described temperature sensor is electrically connected with pcb board.
11. streaming fluorescence detectors as claimed in claim 1, it is characterized in that described optical table is fixed on described support body by some dampings, described damping comprises beam, lower beam, sleeve and vibration-damping screw nail, described optical table is provided with slotted eye, the upper surface of described optical table is provided with a circle depression around described slotted eye, described upper beam is located in described depression, between the lower surface that described optical table is located at by described lower beam and the upper surface of support body, described sleeve is successively through described upper beam, slotted eye and lower beam, the height of described sleeve is less than or equal to lower beam height and optical table height sum, described vibration-damping screw nail is from top to down through described sleeve, and run through described support body, fixed by set nut again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521094475.8U CN205229051U (en) | 2015-12-26 | 2015-12-26 | STREAMING fluorescence detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521094475.8U CN205229051U (en) | 2015-12-26 | 2015-12-26 | STREAMING fluorescence detector |
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| Publication Number | Publication Date |
|---|---|
| CN205229051U true CN205229051U (en) | 2016-05-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201521094475.8U Withdrawn - After Issue CN205229051U (en) | 2015-12-26 | 2015-12-26 | STREAMING fluorescence detector |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105388139A (en) * | 2015-12-26 | 2016-03-09 | 嘉兴凯实生物科技有限公司 | Flow-type fluorescence detector |
-
2015
- 2015-12-26 CN CN201521094475.8U patent/CN205229051U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105388139A (en) * | 2015-12-26 | 2016-03-09 | 嘉兴凯实生物科技有限公司 | Flow-type fluorescence detector |
| CN105388139B (en) * | 2015-12-26 | 2018-03-27 | 嘉兴凯实生物科技有限公司 | A kind of streaming fluorescence detector |
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Granted publication date: 20160511 Effective date of abandoning: 20180327 |