CN105441426A - 一种外周血游离dna靶向富集的方法及试剂盒 - Google Patents
一种外周血游离dna靶向富集的方法及试剂盒 Download PDFInfo
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- CN105441426A CN105441426A CN201511027421.4A CN201511027421A CN105441426A CN 105441426 A CN105441426 A CN 105441426A CN 201511027421 A CN201511027421 A CN 201511027421A CN 105441426 A CN105441426 A CN 105441426A
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- primer
- hybridized
- dna
- peripheral blood
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- 238000012360 testing method Methods 0.000 claims abstract description 10
- 238000001514 detection method Methods 0.000 claims abstract description 9
- 238000011144 upstream manufacturing Methods 0.000 claims description 25
- 108090000623 proteins and genes Proteins 0.000 claims description 15
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- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 6
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- 108020004414 DNA Proteins 0.000 description 51
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- 238000003745 diagnosis Methods 0.000 description 6
- 101001027207 Homo sapiens Kelch-like protein 40 Proteins 0.000 description 4
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- 101150059736 SRY gene Proteins 0.000 description 4
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- 108091061744 Cell-free fetal DNA Proteins 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010052779 Transplant rejections Diseases 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 210000002593 Y chromosome Anatomy 0.000 description 1
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- 231100000693 bioaccumulation Toxicity 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
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- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
Description
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201511027421.4A CN105441426B (zh) | 2015-12-31 | 2015-12-31 | 一种外周血游离dna靶向富集的方法及试剂盒 |
Applications Claiming Priority (1)
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CN201511027421.4A CN105441426B (zh) | 2015-12-31 | 2015-12-31 | 一种外周血游离dna靶向富集的方法及试剂盒 |
Publications (2)
Publication Number | Publication Date |
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CN105441426A true CN105441426A (zh) | 2016-03-30 |
CN105441426B CN105441426B (zh) | 2018-07-03 |
Family
ID=55552059
Family Applications (1)
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CN201511027421.4A Active CN105441426B (zh) | 2015-12-31 | 2015-12-31 | 一种外周血游离dna靶向富集的方法及试剂盒 |
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CN (1) | CN105441426B (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108504653A (zh) * | 2018-04-12 | 2018-09-07 | 东莞博奥木华基因科技有限公司 | 一种母体外周血血浆快速分离方法及其应用 |
CN110846382A (zh) * | 2019-11-29 | 2020-02-28 | 北京科迅生物技术有限公司 | 胎儿游离dna的富集方法 |
CN112899357A (zh) * | 2021-01-05 | 2021-06-04 | 南京普济生物有限公司 | 一种运用数字pcr进行胎儿分数检测的方法及其试剂盒 |
-
2015
- 2015-12-31 CN CN201511027421.4A patent/CN105441426B/zh active Active
Non-Patent Citations (4)
Title |
---|
CHUNMING DING等: "MS analysis of single-nucleotide differences in circulating nucleic acids: Application to noninvasive prenatal diagnosis", 《PNAS》 * |
汪钦: "乙型肝炎治疗耐药基因突变的焦磷酸测序诊断技术研究", 《中国优秀硕士学位论文全文数据库(医药卫生科技辑)》 * |
胡子有等: "焦磷酸测序法在败血症常见病原体检测中的应用", 《南方医科大学学报》 * |
赵之恭等: "免疫磁珠富集母体外周血胎儿DNA方法的研究", 《中华血液学杂志》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108504653A (zh) * | 2018-04-12 | 2018-09-07 | 东莞博奥木华基因科技有限公司 | 一种母体外周血血浆快速分离方法及其应用 |
CN110846382A (zh) * | 2019-11-29 | 2020-02-28 | 北京科迅生物技术有限公司 | 胎儿游离dna的富集方法 |
CN110846382B (zh) * | 2019-11-29 | 2023-02-28 | 北京科迅生物技术有限公司 | 胎儿游离dna的富集方法 |
CN112899357A (zh) * | 2021-01-05 | 2021-06-04 | 南京普济生物有限公司 | 一种运用数字pcr进行胎儿分数检测的方法及其试剂盒 |
Also Published As
Publication number | Publication date |
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CN105441426B (zh) | 2018-07-03 |
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Effective date of registration: 20181213 Address after: 311100 Second Floor, Building 9, 355 Xingzhong Road, Yuhang Economic and Technological Development Zone, Hangzhou City, Zhejiang Province Patentee after: ZHEJIANG DIPU DIAGNOSIS TECHNOLOGY Co.,Ltd. Address before: 310030 33 buildings, North Xihu District business park, Xihu District, Hangzhou, Zhejiang Patentee before: HANGZHOU D. A. GENETIC ENGINEERING Co.,Ltd. |
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Denomination of invention: Method and kit for targeted enrichment of free DNA of peripheral blood Effective date of registration: 20190505 Granted publication date: 20180703 Pledgee: Hangzhou joint rural commercial bank Limited by Share Ltd. three pier sub branch Pledgor: ZHEJIANG DIPU DIAGNOSIS TECHNOLOGY Co.,Ltd. Registration number: 2019330000118 |
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Date of cancellation: 20210916 Granted publication date: 20180703 Pledgee: Hangzhou joint rural commercial bank Limited by Share Ltd. three pier sub branch Pledgor: ZHEJIANG DIPU DIAGNOSIS TECHNOLOGY Co.,Ltd. Registration number: 2019330000118 |
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PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
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Denomination of invention: A method and kit for targeted enrichment of free DNA in peripheral blood Effective date of registration: 20211020 Granted publication date: 20180703 Pledgee: Hangzhou joint rural commercial bank Limited by Share Ltd. three pier sub branch Pledgor: ZHEJIANG DIPU DIAGNOSIS TECHNOLOGY Co.,Ltd. Registration number: Y2021330001971 |
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Date of cancellation: 20220829 Granted publication date: 20180703 Pledgee: Hangzhou joint rural commercial bank Limited by Share Ltd. three pier sub branch Pledgor: ZHEJIANG DIPU DIAGNOSIS TECHNOLOGY Co.,Ltd. Registration number: Y2021330001971 |
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