CN105441327A - Hydrolyzed fish soluble microbial culture medium - Google Patents
Hydrolyzed fish soluble microbial culture medium Download PDFInfo
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- CN105441327A CN105441327A CN201510987414.2A CN201510987414A CN105441327A CN 105441327 A CN105441327 A CN 105441327A CN 201510987414 A CN201510987414 A CN 201510987414A CN 105441327 A CN105441327 A CN 105441327A
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- Prior art keywords
- culture medium
- microbial culture
- soup
- microbial
- fish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
Abstract
The invention discloses a hydrolyzed fish soluble microbial culture medium. The microbial culture medium is applied to a microbial culture medium in large-scale production. The microbial culture medium is prepared from molasses, hydrolyzed fish soluble, potassium dihydrogen phosphate and magnesium sulfate, manganese sulfate, ammonium sulfate or calcium carbonate. The microbial culture medium is applicable to microbial fermentation industry; and when the microbial culture medium is used, the microbial culture medium can be applied to microbial incremental culture after being uniformly mixed with sterile water according to a corresponding proportion. The hydrolyzed fish soluble microbial culture medium is convenient to use and low in cost, can be used for well solving the problem of carbon source and nitrogen source in the culture medium, and can achieve the aim of environment protection while changing waste into valuables since industrial leftovers are applied as raw materials. The microbial culture medium is prepared from raw materials having lower price and wider source than those of the conventional bacterium culture which uses beef extract, yeast extract and peptone, and has comprehensive amino acid, vitamins and trace element nutrition.
Description
Technical field
Disclosed by the invention is a kind of hydrolysis fish slurry microbiological culture media, belongs to biological technical field.
Background technology
At present; at the microorganism increment of fermentable industrial application; what take is the method increased step by step; the substratum adopted is all use by the culture medium prescription Extemporaneous in laboratory; raw material conventional in laboratory culture based formulas is extractum carnis, yeast extract paste, peptone and agar etc.; these cost of material are higher; when laboratory condition uses on a small quantity; cost burden is not very heavy; but when applying as large-scale production; need a large amount of substratum, at this moment culture medium cost inherently becomes the heavy burden of enterprise.Therefore, seek a kind of hydrolysis fish slurry substratum of low cost, for reduction large-scale production microorganism culturing cost, just seem very necessary.But, also do not find that the report of being correlated with and document disclose in this respect.As everyone knows, the spawn culture Main Nutrients of microbiological culture media is carbon source, nitrogenous source, then coordinates other elements.As long as filter out suitable Carbon and nitrogen sources, substantially can prepare qualified microbiological culture media, follow this thinking and just hydrolysis fish slurry microbiological culture media can be prepared.
Summary of the invention
Object of the present invention is just to provide a kind of hydrolysis fish slurry microbiological culture media; microbiological culture media is prepared with new Carbon and nitrogen sources surrogate; when overcoming increment microorganism in traditional large-scale production; just the use existing for raw material Extemporaneous of reference laboratories substratum is inconvenient, the deficiency that cost is high.
Foregoing invention object, is realized by following concrete technical scheme,
a kind ofhydrolysis fish slurry
microbiological culture media, prepare primarily of following weight parts raw material:
Molasses 14-56 part, hydrolysis fish slurry 40-85 part, potassium primary phosphate 0.5-5 part, salt 0.3-6.5 part;
Described salt refers to two or three of magnesium sulfate, manganous sulfate, ammonium sulfate or calcium carbonate;
By said components preparation, mix, be warming up to 121 degree of autoclave sterilizations 30 minutes, be cooled to normal temperature and pack to obtain finished product;
Described sugar is close refer to sugarcane squeeze sugar after byproduct, be commonly called as tangerine water;
Described hydrolysis fish slurry refers to the soup compound that the soup after with raw fish and the processing of aquatic products shellfish meat is prepared for raw material, and its preparation method is:
(1) after boiling of being heated by raw fish is ripe, carry out squeezing slurry, obtain fish slurry for subsequent use;
(2) soup after water intaking product source mill processing shellfish meat, concentrates and removes 80 ﹪ moisture content, obtain soup thickened pulp;
(3) fish slurry and soup thickened pulp are mixed in 1:3 ratio, and regulate pH value to be 5-6, the papoid of enzyme activity 90-120 ten thousand U/g is added in the ratio of 1.5-2 ‰, Keep agitation, 55-60 DEG C of hydrolysis after 12 hours, be warming up to more than the 90 DEG C enzymes 30 minutes of going out again, obtain hydrolyzed solution for subsequent use;
(4) hydrolyzed solution being concentrated into protein content is 35-40 ﹪, namely obtains hydrolysis fish slurry.
Take the present invention of above-mentioned measure; be applicable to fermentable industry; in use by corresponding proportioning add sterilized water mix namely can be applicable to microorganism increment cultivate; easy to use; adult is cheap; solve the problem of carbon source and nitrogenous source in substratum well, because applicable industry tankage are as raw material, while turning waste into wealth, reach the object of environment protection.Carbon source molasses of the present invention, cheaply should to obtain, nitrogenous source derives from the soup after raw fish and the processing of shellfish meat, soup after raw fish and the processing of shellfish meat is rich in amino acid and the trace element of albumen egg, VITAMIN and needed by human, it is the extremely valuable protein source of culturing micro-organisms, soup microbe conversion after being processed with shellfish meat by raw fish slurry becomes fish protein hy concentrated nutrition to starch i.e. hydrolysis fish slurry, as the nitrogenous source of spawn culture, comprehensive utilization of resources is that the one of spawn culture protein source substitutes innovation.The conventional cultivation of raw material ratio of the present invention uses extractum carnis, yeast extract paste and peptone price low, and source is wide, and amino acid, VITAMIN and micronutrient are comprehensively abundant.
Embodiment
Embodiment 1
The hydrolysis fish slurry medium preparing of yeast and mould
(1) preparation of fish slurry is hydrolyzed:
(1) get 100 kilograms of fresh raw fishs heat boiling ripe after, carry out squeezing slurry, obtain 100 kilograms of fishes slurry for subsequent use;
(2) 1500 kilograms, soup after water intaking product source mill processing shellfish meat, concentrates and removes 80 ﹪ moisture content, obtain 300 kilograms of soup thickened pulp;
(3) by fish slurry and the mixing of soup thickened pulp, adjustment pH value is 5-6, adds the papoid of enzyme activity 90-120 ten thousand U/g, Keep agitation, is hydrolyzed after 12 hours at 55-60 DEG C, then is warming up to more than the 90 DEG C enzymes 30 minutes of going out, and obtains hydrolyzed solution for subsequent use;
(4) hydrolyzed solution being concentrated into protein content is 35-40 ﹪, namely obtains hydrolysis fish slurry;
(2) fill a prescription
56 kilograms, molasses, hydrolysis fish starch 42.7 kilograms, potassium primary phosphate 0.7 kilogram, 0.35 kilogram, ammonium sulfate, 0.25 kilogram, magnesium sulfate;
(3) fish slurry medium preparation method is hydrolyzed
By said components preparation, mix, be warming up to 121 degree of autoclave sterilizations 30 minutes, be cooled to normal temperature and pack to obtain finished product;
Embodiment 2
The hydrolysis fish slurry medium preparing of genus bacillus
One, the preparation of fish slurry is hydrolyzed identical with the step () of embodiment 1;
Two, fill a prescription
Hydrolysis fish starch 85 kilograms, 14 kilograms, molasses, potassium primary phosphate 0.7 kilogram, 0.25 kilogram, magnesium sulfate, manganous sulfate 0.05 kilogram;
(3) fish slurry medium preparation method is hydrolyzed identical with the step (three) of embodiment 1.
Embodiment 3
The hydrolysis fish slurry medium preparing of milk-acid bacteria
(1) preparation of fish slurry is hydrolyzed identical with the step () of embodiment 1;
(2) fill a prescription:
46 kilograms, molasses, hydrolysis fish starch 46 kilograms, potassium primary phosphate 5 kilograms, 2 kilograms, magnesium sulfate, manganous sulfate 1 kilogram;
(3) fish slurry medium preparation method is hydrolyzed identical with the step (three) of embodiment 1.
Embodiment 4
Actinomycetic hydrolysis fish slurry medium preparing
(1) preparation of fish slurry is hydrolyzed identical with the step () of embodiment 1;
(2) fill a prescription
47 kilograms, molasses, hydrolysis fish starch 46.5 kilograms, 4 kilograms, ammonium sulfate, 1.5 kilograms, calcium carbonate, dipotassium hydrogen phosphate 0.5 kilogram, 0.5 kilogram, magnesium sulfate.
(3) fish slurry medium preparation method is hydrolyzed identical with the step (three) of embodiment 1.
Use embodiment 5
Above-described embodiment product using method: when cultivating bacterial classification, get corresponding finished product substratum, 8-10 kilogram of preparation 1 ton of bacteria culture fluid.Implementation method is by the conventional production operation guidelines of respective factory.
Claims (1)
1. be hydrolyzed a fish slurry microbiological culture media, it is characterized in that described substratum is prepared primarily of following weight parts raw material:
Molasses 14-56 part, hydrolysis fish slurry 40-85 part, potassium primary phosphate 0.5-5 part, salt 0.3-6.5 part;
Described salt refers to two or three of magnesium sulfate, manganous sulfate, ammonium sulfate or calcium carbonate;
By said components preparation, mix, be warming up to 121 degree of autoclave sterilizations 30 minutes, be cooled to normal temperature and pack to obtain finished product;
Described hydrolysis fish slurry refers to the soup compound that the soup after with raw fish and the processing of aquatic products shellfish meat is prepared for raw material, and its preparation method is:
(1) after boiling of being heated by raw fish is ripe, carry out squeezing slurry, obtain fish slurry for subsequent use;
(2) soup after water intaking product source mill processing shellfish meat, concentrates and removes 80 ﹪ moisture content, obtain soup thickened pulp;
(3) fish slurry and soup thickened pulp are mixed in 1:3 ratio, and regulate pH value to be 5-6, the papoid of enzyme activity 90-120 ten thousand U/g is added in the ratio of 1.5-2 ‰, Keep agitation, 55-60 DEG C of hydrolysis after 12 hours, be warming up to more than the 90 DEG C enzymes 30 minutes of going out again, obtain hydrolyzed solution for subsequent use;
(4) hydrolyzed solution being concentrated into protein content is 35-40 ﹪, namely obtains hydrolysis fish slurry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510987414.2A CN105441327A (en) | 2015-12-27 | 2015-12-27 | Hydrolyzed fish soluble microbial culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510987414.2A CN105441327A (en) | 2015-12-27 | 2015-12-27 | Hydrolyzed fish soluble microbial culture medium |
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| Publication Number | Publication Date |
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| CN105441327A true CN105441327A (en) | 2016-03-30 |
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| CN201510987414.2A Pending CN105441327A (en) | 2015-12-27 | 2015-12-27 | Hydrolyzed fish soluble microbial culture medium |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102067940A (en) * | 2010-11-12 | 2011-05-25 | 薛晓宁 | Fish soluble feed protein and processing method thereof |
| CN102746999A (en) * | 2011-04-19 | 2012-10-24 | 张金松 | LH microbes and their preparation method and use |
| CN103667407A (en) * | 2012-09-07 | 2014-03-26 | 天津拓飞生物科技有限公司 | Polypeptide-zinc metal complex as well as preparation method and application thereof |
-
2015
- 2015-12-27 CN CN201510987414.2A patent/CN105441327A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102067940A (en) * | 2010-11-12 | 2011-05-25 | 薛晓宁 | Fish soluble feed protein and processing method thereof |
| CN102746999A (en) * | 2011-04-19 | 2012-10-24 | 张金松 | LH microbes and their preparation method and use |
| CN103667407A (en) * | 2012-09-07 | 2014-03-26 | 天津拓飞生物科技有限公司 | Polypeptide-zinc metal complex as well as preparation method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 万菡等: "草鱼内脏水解蛋白功能性质及对微生物的培养效果研究", 《武汉工业学院学报》 * |
| 梁志宏等: "《食品微生物学实验》", 31 December 2013, 中国林业出版社 * |
| 王伟东等: "《农业生物技术教程》", 31 August 2015, 中国农业大学出版社 * |
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Application publication date: 20160330 |